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Christophe Gadenne - One of the best experts on this subject based on the ideXlab platform.
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mating induced differential peptidomics of neuropeptides and protein hormones in Agrotis Ipsilon moths
Journal of Proteome Research, 2018Co-Authors: Max Diesner, Joachim Schachtner, Jorg Kahnt, Aurore Gallot, Hellena Binz, Cyril Gaertner, Simon Vitecek, Emmanuelle Jacquinjoly, Christophe GadenneAbstract:In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis Ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. Ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal l...
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Mating-induced differential peptidomics of neuropeptides and protein hormones in Agrotis Ipsilon moths
Journal of Proteome Research, 2018Co-Authors: Max Diesner, Joachim Schachtner, Jorg Kahnt, Aurore Gallot, Hellena Binz, Cyril Gaertner, Simon Vitecek, Emmanuelle Jacquin Joly, Christophe GadenneAbstract:In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis Ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. Ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal lobes, the gnathal ganglion, and the corpora cardiaca-corpora allata complex. Comparisons between virgin and mated males and females revealed tissue-specific differences in peptide composition between sexes and according to physiological state. Mated males showed postmating differences in neuropeptide occurrence, which could participate in the mating-induced olfactory plasticity.
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Mating-Induced Differential Peptidomics of Neuropeptides and Protein Hormones in Agrotis Ipsilon Moths
2018Co-Authors: Max Diesner, Joachim Schachtner, Jorg Kahnt, Aurore Gallot, Hellena Binz, Cyril Gaertner, Simon Vitecek, Emmanuelle Jacquin Joly, Christophe GadenneAbstract:In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis Ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. Ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal lobes, the gnathal ganglion, and the corpora cardiaca–corpora allata complex. Comparisons between virgin and mated males and females revealed tissue-specific differences in peptide composition between sexes and according to physiological state. Mated males showed postmating differences in neuropeptide occurrence, which could participate in the mating-induced olfactory plasticity
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three dimensional antennal lobe atlas of the male moth Agrotis Ipsilon a tool to study structure function correlation
The Journal of Comparative Neurology, 2004Co-Authors: Birgit Greiner, Christophe Gadenne, Sylvia AntonAbstract:The glomerular structure of the primary olfactory neuropil has long been thought to play an important role in odour coding. In insects, the number of glomeruli in the antennal lobe is limited in most species to fewer than 100 compared with more than 1,000 in vertebrates, making it possible to identify individual glomeruli. A complete three-dimensional atlas of the glomeruli within the antennal lobe of the male noctuid moth Agrotis Ipsilon was constructed. All 66 glomeruli were singly identifiable in both antennal lobes of the three brains investigated. Further, six antennal lobes containing intracellularly stained projection neurones were reconstructed. By using the atlas, the respective target glomerulus of each projection neurone could be identified. The importance of the glomerular atlas as a tool to study central olfactory processing and its plasticity is discussed.
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evolution of noctuid pheromone binding proteins identification of pbp in the black cutworm moth Agrotis Ipsilon
Insect Biochemistry and Molecular Biology, 2002Co-Authors: Jean-françois Picimbon, Christophe GadenneAbstract:Male black cutworm moths (Agrotis Ipsilon, Lepidoptera, Noctuoidea, Noctuidae), which are attracted by a three-component pheromone blend ((Z)-7-dodecenyl acetate, Z7-12:Ac; (Z)-9-tetradecenyl acetate, Z9-14:Ac; (Z)-11-hexadecenyl acetate, Z11-16:Ac), express diverse antennal pheromone binding proteins (PBPs). Two PBP isoforms (Aips-1 and Aips-2) that show 46% identity were cloned from antennal cDNA of male A. Ipsilon. The protein Aips-1 displays a high degree of identity (70-95%) with PBPs of other noctuiids, but shows only 42-65% identity with the PBPs of more phylogenetically distant species. The other protein, Aips-2, represents a distinct group of PBP that includes proteins from Sphingidae and Yponomeutidae. These differences observed suggest that each of the two PBPs may be tuned to a specific pheromone ligand.
Bryony C. Bonning - One of the best experts on this subject based on the ideXlab platform.
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toward the physiological basis for increased Agrotis Ipsilon multiple nucleopolyhedrovirus infection following feeding of Agrotis Ipsilon larvae on transgenic corn expressing cry1fa2
Journal of Invertebrate Pathology, 2009Co-Authors: Nina Schmidt, Jessica M Haywood, Bryony C. BonningAbstract:Larvae of the black cutworm, Agrotis Ipsilon Hufnagel, were more susceptible to infection by A. Ipsilon multiple nucleopolyhedrovirus (AgipMNPV: Baculoviridae) after feeding on Herculex I, a transgenic corn hybrid expressing the Bacillus thuringiensis (Bt)-derived toxin Cry1Fa2 compared to larvae fed on isoline corn. We investigated the physiological basis for increased susceptibility to virus infection following exposure to Herculex I by analyzing the midgut pH, gut protease activity and peritrophic matrix structure which are important factors for both Bt toxin action and baculovirus infection. No significant treatment differences were found in the pH of anterior midgut, central midgut or posterior midgut in larvae fed Herculex I or isoline diets. Analysis of soluble and membrane-associated gut proteinase activities from larvae fed Herculex I or isoline diets indicated that membrane-associated aminopeptidase activity and soluble chymotrypsin-like proteinase activity were significantly lower in Herculex I -fed larvae compared to isoline-fed larvae. The number and relative molecular masses of soluble chymotrypsin-like proteinases did not differ. Baculoviruses were not susceptible to in vitro degradation by bovine chymotrypsin, suggesting that chymotrypsin degradation of baculovirus occlusion-derived virus did not result in reduced infection of larvae fed on isoline diet. Scanning electron micrographs of the peritrophic matrices of Herculex I -fed larvae and isoline-fed larvae indicated that Herculex I did not result in damage to the peritrophic matrix that could facilitate subsequent baculovirus infection. Additional research is required to further delineate the physiological basis for enhanced baculovirus infection following exposure to sublethal doses of Bt toxins.
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microbial control of black cutworm lepidoptera noctuidae in turfgrass using Agrotis Ipsilon multiple nucleopolyhedrovirus
Journal of Economic Entomology, 2006Co-Authors: Callie A Prater, Carl T Redmond, Walter E Barney, Bryony C. BonningAbstract:Agrotis Ipsilon multiple nucleopolyhedrovirus (family Baculoviridae, genus Nucleopolyhedrovirus, AgipMNPV), a naturally occurring baculovirus, was found infecting black cutworm, Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae), on central Kentucky golf courses. Laboratory, greenhouse, and field studies investigated the potential of AgipMNPV for managing black cutworms in turfgrass. The virus was highly active against first instars (LC50 = 73 occlusion bodies [OBs] per microl with 2-microl dose; 95% confidence intervals, 55-98). First instars that ingested a high lethal dose stopped feeding and died in 3-6 d as early second instars, whereas lethally infected fourth instars continued to feed and grow for 4-9 d until death. Sublethal doses consumed by third or fifth instars had little or no effect on subsequent developmental rate or pupal weight. Horizontal transmission of AgipMNPV in turfgrass plots was shown. Sprayed suspensions of AgipMNPV (5 x 10(8) - 6 x 10(9) OBs/m2) resulted in 75 to > 93% lethal infection of third or fourth instars in field plots of fairway-height creeping bentgrass, Agrostis stolonifera (Huds.), and on a golf course putting green collar. Virus spray residues (7 x 10(9) OBs/m2) allowed to weather on mowed and irrigated creeping bentgrass field plots significantly increased lethal infection of implanted larvae for at least 4 wk. This study, the first to evaluate a virus against a pest in turfgrass, suggests that AgipMNPV has potential as a preventive bioinsecticide targeting early instar black cutworms. Establishing a virus reservoir in the thatch and soil could suppress successive generations of that key pest on golf courses and sport fields.
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potential of Agrotis Ipsilon nucleopolyhedrovirus for suppression of the black cutworm lepidoptera noctuidae and effect of an optical brightener on virus efficacy
Journal of Economic Entomology, 2001Co-Authors: Anthony J Boughton, Leslie C. Lewis, Bryony C. BonningAbstract:Abstract Studies were performed in the laboratory, greenhouse and field to assess the potential of Agrotis Ipsilon multicapsid nucleopolyhedrovirus (AgipMNPV) and a viral enhancing agent, M2R, for suppression of Agrotis Ipsilon (Hufnagel). In laboratory droplet feeding bioassays, AgipMNPV was shown to be highly active against third-instar A. Ipsilon. The optical brightener M2R significantly reduced LD50 estimates by ≈160-fold, but had no direct effect on survival time estimates. In greenhouse trials, spray and bait formulations of AgipMNPV significantly reduced feeding damage to corn seedlings caused by third-instar A. Ipsilon. In two sets of replicated field trials, bait formulations of AgipMNPV significantly reduced feeding damage to corn seedlings by third-instar A. Ipsilon. However, there were no beneficial effects attributable to the inclusion of M2R in AgipMNPV formulations under greenhouse or field conditions. It seems likely that in an appropriately designed pest management program AgipMNPV could ...
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Characterization of a nucleopolyhedrovirus from the black cutworm Agrotis Ipsilon (Lepidoptera: Noctuidae)
Journal of invertebrate pathology, 1999Co-Authors: Anthony J Boughton, Leslie C. Lewis, Robert L. Harrison, Bryony C. BonningAbstract:The black cutworm, Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae), is a serious localized pest of vegetable and field crops. We have characterized a newly discovered baculovirus, the Agrotis Ipsilon multicapsid nucleopolyhedrovirus (AgipMNPV), that was isolated from A. Ipsilon in Illinois. Restriction enzyme fragment profiles of AgipMNPV DNA were distinct from those of previously described nucleopolyhedroviruses. Electron microscopy of AgipMNPV-infected tissues indicated that nucleocapsids of this virus are multiply enveloped. A. Ipsilon was highly susceptible to infection by AgipMNPV and significantly more susceptible to infection by AgipMNPV than by Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV). Host range studies showed that Heliothis virescens and Helicoverpa zea were moderately susceptible to infection; Pseudaletia unipuncta and Spodoptera frugiperda were only partially susceptible, and Anticarsia gemmatalis, Spodoptera exigua, Trichoplusia ni, and Ostrinia nubilalis were not susceptible to infection by AgipMNPV. Because of its high virulence, AgipMNPV has potential as an alternative to chemical insecticides for control of A. Ipsilon. r 1999 Academic Press
Jean-françois Picimbon - One of the best experts on this subject based on the ideXlab platform.
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Interpopulational Variations of Odorant-Binding Protein Expression in the Black Cutworm Moth, Agrotis Ipsilon.
Insects, 2020Co-Authors: Jean-françois PicimbonAbstract:A long-range migrant species of moth (Agrotis Ipsilon) has served as a model to compare the expression profiles of antennal proteins between different continental populations. Our results showed that the American and French populations of the black cutworm moth, A. Ipsilon, expressed the same odorant-binding proteins (OBPs), but apparently in different levels. Electrophoretic analysis of antennal protein profiles and reverse transcription polymerase chain reaction using RNA as a template showed significant differences between the two populations in the expression of antennal binding protein-X (ABPX) and general odorant-binding protein-2 (GOBP2). However, the two A. Ipsilon populations showed no differences in RNA levels coding for pheromone binding proteins (PBPs), suggesting that the expression of generalist OBPs is population-specific and could be affected by specific odor and/or chemical changes in external environmental conditions. To support the role of ABPX and GOBP2 with expression, the role of ABPX and GOBP2 is discussed in regard to odor detection, memorization and/or degradation of toxic chemical insecticides.
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evolution of noctuid pheromone binding proteins identification of pbp in the black cutworm moth Agrotis Ipsilon
Insect Biochemistry and Molecular Biology, 2002Co-Authors: Jean-françois Picimbon, Christophe GadenneAbstract:Male black cutworm moths (Agrotis Ipsilon, Lepidoptera, Noctuoidea, Noctuidae), which are attracted by a three-component pheromone blend ((Z)-7-dodecenyl acetate, Z7-12:Ac; (Z)-9-tetradecenyl acetate, Z9-14:Ac; (Z)-11-hexadecenyl acetate, Z11-16:Ac), express diverse antennal pheromone binding proteins (PBPs). Two PBP isoforms (Aips-1 and Aips-2) that show 46% identity were cloned from antennal cDNA of male A. Ipsilon. The protein Aips-1 displays a high degree of identity (70-95%) with PBPs of other noctuiids, but shows only 42-65% identity with the PBPs of more phylogenetically distant species. The other protein, Aips-2, represents a distinct group of PBP that includes proteins from Sphingidae and Yponomeutidae. These differences observed suggest that each of the two PBPs may be tuned to a specific pheromone ligand.
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Development and pheromone communication systems in hybrids of Agrotis Ipsilon and Agrotis segetum (Lepidoptera : Noctuidae)
Journal of Chemical Ecology, 1997Co-Authors: Christophe Gadenne, Jean-françois Picimbon, Jean-marc Becard, B. Lalanne-cassou, Michel RenouAbstract:Hybrids were obtained by crossing males of the turnip moth Agrotis segetum, which has a period of arrested development in the larval stage, with females of the migrant and nondiapausing black cutworm, Agrotis Ipsilon. No progeny were obtained by crossing females of A. segetum with males of A. Ipsilon. Backcrosses were successful only by crossing hybrids with both sexes of A. Ipsilon. No larval oligopause occurred when hybrid larvae were reared under conditions inducing arrest in A. segetum (12L:12D, 21°C). Wind tunnel tests showed interattractiveness of F1 hybrids with A. Ipsilon but not with A. segetum. Single sensillum recordings of pheromone-sensitive hairs on the antennae of males of the parent species revealed differences in the distribution of sensilla. F1 and F2 hybrids exhibited the same receptor cell types and distribution as A. Ipsilon. Gas chromatographic analysis of female pheromone gland extracts revealed that F1 hybrids were also very close to A. Ipsilon.
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Juvenile hormone stimulates pheromonotropic brain factor release in the female Black cutworm, Agrotis Ipsilon
Journal of Insect Physiology, 1995Co-Authors: Jean-françois Picimbon, Leam Sreng, Jean-marc Becard, Jean-luc Clément, Christophe GadenneAbstract:Sex pheromone biosynthesis of various insects has been shown to be controlled by a brain neuropeptide (PBAN) and in some cases by juvenile hormone (JH) from the corpora allata (CA), However, the mode of action of JH in the control of sex pheromone biosynthesis is still unclear, Although pheromonotropic activity was detected in their brains, females of the black cutworm, Agrotis Ipsilon, lacking CA did not produce sex pheromone, Pheromone production could be restored in allatectomized female moths by injection of brain extracts or by injection of synthetic PBAN or JH-III, Injection of synthetic JH-III did not stimulate pheromone production in decapitated females. These results indicate that JH secreted by CA control sex pheromone biosynthesis of A, Ipsilon females by stimulating pheromonotropic brain factor (PBAN-like factor) release from the central nervous system and not by controlling PBAN-like production or by priming PBAN-like target glandular cells,
Ingrid Schimidt Kaiser - One of the best experts on this subject based on the ideXlab platform.
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Toxicity of Bacillus thuringiensis at different larval ages of Agrotis Ipsilon (Lepidoptera: Noctuidae).
Revista de Ciências Agroveterinárias, 2020Co-Authors: Ingrid Schimidt Kaiser, Dirceu Pratissoli, Victor Luiz De Souza Lima, Lorena Contarini Machado, Regiane Cristina Oliveira De Freitas BuenoAbstract:The black cutworm Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae) is a cosmopolitan and polyphagous pest that attacks diverse crops and weed. One of the alternatives to insecticides may be the use of bioinsecticides based on Bacillus thuringiensis Berliner (Bt). Thus, the objective of the present study were evaluating the toxicity of Agree® and Dipel® bioinsecticides based on Bt on different larval ages of A. Ipsilon. For the experiments, five larval ages were used (0-24, 48-72, 96-120, 144-168, and 192-216 h). The A. Ipsilon caterpillars were individualized in acrylic tubes containing an artificial diet and 50 μL of each biopesticide in the concentration 1 × 108 spores mL-1. Mortality was assessed for seven days. The two bioinsecticides evaluated promoted mortality at all larval ages of A. Ipsilon. The age of 0-24 h had mortality above 90%. The values of LC50 and LC90 were 9.8 × 105 and 7.4 × 106 spores mL-1 for Agree® and 1.3 × 106 e 1.4 × 107 spores mL-1 for Dipel®, respectively, without difference between LC50 and LC90 values of the bioinsecticides. The results indicate that younger caterpillars are more susceptible to Bt-based bioinsecticides.
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PERSISTÊNCIA DE Steinernema carpocapsae E S. feltiae (RHABDITIDA: STEINERNEMATIDAE) NO SOLO SOBRE Agrotis Ipsilon (LEPIDOPTERA: NOCTUIDAE)
2018Co-Authors: Ingrid Schimidt Kaiser, Dirceu Pratissoli, Victor Luiz De Souza Lima, Alixelhe Pacheco Damascena, Regiane Cristina Oliveira De Freitas BuenoAbstract:The black cutworm Agrotis Ipsilon (Lepidoptera: Noctuidae) is a polyphagous pest that attacks more than one hundred types of crops. The habit of being buried or underneath the cultural remains may help contact with entomopathogenic nematodes (EPNs), which are natural enemies of insects. The objective of this study was to evaluate the persistence of the pathogenicity of Steinernema carpocapsae and S. feltiae (Rhabditida: Steinernematidae) applied to the soil against A. Ipsilon larvae. The EPNs were applied to vases containing soil and larvae of A. Ipsilon were introduced into the pots in five periods (0, 2, 4, 6, and 8 days after treatment). The two species of EPNs caused pathogenicity in all evaluated periods. The mortality rate was over 92% in all treatments and periods.
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efeito de oleos essenciais citricos sobre Agrotis Ipsilon hufnagel lepidoptera noctuidae
Revista UniVap, 2017Co-Authors: Ingrid Schimidt Kaiser, Sabrina Buqueroni Alves, Breno Benvindo Dos Anjos, Débora Ferreira Melo Fragoso, Julielson Oliveira Ataide, Dirceu PratissoliAbstract:A utilizacao de plantas inseticidas e cada vez mais frequente no controle de pragas. As plantas podem produzir diferentes compostos com atividade inseticida. Os oleos essenciais destacam-se dentre estes compostos, possuindo diferentes modos de acao inseticida, podendo ser uma ferramenta util no manejo integrado de pragas. O objetivo deste trabalho e avaliar o efeito de oleos essenciais citricos e seu composto majoritario D-limonemo visando sua adocao como metodos alternativos de controle de Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae). Para a realizacao do experimento foram utilizadas 50 lagartas de 1o instar por tratamento. Foram aplicados os oleos essenciais com auxilio do aerografo, sendo utilizado na concentracao de 10% v v -1 para os oleos citricos e o D-limoneno e para testemunha foi pulverizado agua destilada. A avaliacao foi realizada apos 24, 48 e 72 horas verificando o numero de lagartas mortas. Nao foi observado efeito toxico dos oleos essenciais citricos e do D-limoneno sobre as lagartas de A. Ipsilon . Deste modo, os oleos essenciais testados nao sao eficazes no controle da praga.
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EFEITO DE ÓLEOS ESSENCIAIS CÍTRICOS SOBRE Agrotis Ipsilon (HUFNAGEL) (LEPIDOPTERA:NOCTUIDAE)
Universidade do Vale do Paraíba, 2017Co-Authors: Ingrid Schimidt Kaiser, Sabrina Buqueroni Alves, Breno Benvindo Dos Anjos, Débora Ferreira Melo Fragoso, Julielson Oliveira Ataide, Dirceu PratissoliAbstract:A utilização de plantas inseticidas é cada vez mais frequente no controle de pragas. As plantas podem produzir diferentes compostos com atividade inseticida. Os óleos essenciais destacam-se dentre estes compostos, possuindo diferentes modos de ação inseticida, podendo ser uma ferramenta útil no manejo integrado de pragas. O objetivo deste trabalho é avaliar o efeito de óleos essenciais cítricos e seu composto majoritário D-limonemo visando sua adoção como métodos alternativos de controle de Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae). Para a realização do experimento foram utilizadas 50 lagartas de 1º ínstar por tratamento. Foram aplicados os óleos essenciais com auxilio do aerógrafo, sendo utilizado na concentração de 10% v v-1 para os óleos cítricos e o D-limoneno e para testemunha foi pulverizado água destilada. A avaliação foi realizada após 24, 48 e 72 horas verificando o número de lagartas mortas. Não foi observado efeito tóxico dos óleos essenciais cítricos e do D-limoneno sobre as lagartas de A. Ipsilon. Deste modo, os óleos essenciais testados não são eficazes no controle da praga
Dirceu Pratissoli - One of the best experts on this subject based on the ideXlab platform.
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Toxicity of Bacillus thuringiensis at different larval ages of Agrotis Ipsilon (Lepidoptera: Noctuidae).
Revista de Ciências Agroveterinárias, 2020Co-Authors: Ingrid Schimidt Kaiser, Dirceu Pratissoli, Victor Luiz De Souza Lima, Lorena Contarini Machado, Regiane Cristina Oliveira De Freitas BuenoAbstract:The black cutworm Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae) is a cosmopolitan and polyphagous pest that attacks diverse crops and weed. One of the alternatives to insecticides may be the use of bioinsecticides based on Bacillus thuringiensis Berliner (Bt). Thus, the objective of the present study were evaluating the toxicity of Agree® and Dipel® bioinsecticides based on Bt on different larval ages of A. Ipsilon. For the experiments, five larval ages were used (0-24, 48-72, 96-120, 144-168, and 192-216 h). The A. Ipsilon caterpillars were individualized in acrylic tubes containing an artificial diet and 50 μL of each biopesticide in the concentration 1 × 108 spores mL-1. Mortality was assessed for seven days. The two bioinsecticides evaluated promoted mortality at all larval ages of A. Ipsilon. The age of 0-24 h had mortality above 90%. The values of LC50 and LC90 were 9.8 × 105 and 7.4 × 106 spores mL-1 for Agree® and 1.3 × 106 e 1.4 × 107 spores mL-1 for Dipel®, respectively, without difference between LC50 and LC90 values of the bioinsecticides. The results indicate that younger caterpillars are more susceptible to Bt-based bioinsecticides.
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PERSISTÊNCIA DE Steinernema carpocapsae E S. feltiae (RHABDITIDA: STEINERNEMATIDAE) NO SOLO SOBRE Agrotis Ipsilon (LEPIDOPTERA: NOCTUIDAE)
2018Co-Authors: Ingrid Schimidt Kaiser, Dirceu Pratissoli, Victor Luiz De Souza Lima, Alixelhe Pacheco Damascena, Regiane Cristina Oliveira De Freitas BuenoAbstract:The black cutworm Agrotis Ipsilon (Lepidoptera: Noctuidae) is a polyphagous pest that attacks more than one hundred types of crops. The habit of being buried or underneath the cultural remains may help contact with entomopathogenic nematodes (EPNs), which are natural enemies of insects. The objective of this study was to evaluate the persistence of the pathogenicity of Steinernema carpocapsae and S. feltiae (Rhabditida: Steinernematidae) applied to the soil against A. Ipsilon larvae. The EPNs were applied to vases containing soil and larvae of A. Ipsilon were introduced into the pots in five periods (0, 2, 4, 6, and 8 days after treatment). The two species of EPNs caused pathogenicity in all evaluated periods. The mortality rate was over 92% in all treatments and periods.
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efeito de oleos essenciais citricos sobre Agrotis Ipsilon hufnagel lepidoptera noctuidae
Revista UniVap, 2017Co-Authors: Ingrid Schimidt Kaiser, Sabrina Buqueroni Alves, Breno Benvindo Dos Anjos, Débora Ferreira Melo Fragoso, Julielson Oliveira Ataide, Dirceu PratissoliAbstract:A utilizacao de plantas inseticidas e cada vez mais frequente no controle de pragas. As plantas podem produzir diferentes compostos com atividade inseticida. Os oleos essenciais destacam-se dentre estes compostos, possuindo diferentes modos de acao inseticida, podendo ser uma ferramenta util no manejo integrado de pragas. O objetivo deste trabalho e avaliar o efeito de oleos essenciais citricos e seu composto majoritario D-limonemo visando sua adocao como metodos alternativos de controle de Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae). Para a realizacao do experimento foram utilizadas 50 lagartas de 1o instar por tratamento. Foram aplicados os oleos essenciais com auxilio do aerografo, sendo utilizado na concentracao de 10% v v -1 para os oleos citricos e o D-limoneno e para testemunha foi pulverizado agua destilada. A avaliacao foi realizada apos 24, 48 e 72 horas verificando o numero de lagartas mortas. Nao foi observado efeito toxico dos oleos essenciais citricos e do D-limoneno sobre as lagartas de A. Ipsilon . Deste modo, os oleos essenciais testados nao sao eficazes no controle da praga.
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EFEITO DE ÓLEOS ESSENCIAIS CÍTRICOS SOBRE Agrotis Ipsilon (HUFNAGEL) (LEPIDOPTERA:NOCTUIDAE)
Universidade do Vale do Paraíba, 2017Co-Authors: Ingrid Schimidt Kaiser, Sabrina Buqueroni Alves, Breno Benvindo Dos Anjos, Débora Ferreira Melo Fragoso, Julielson Oliveira Ataide, Dirceu PratissoliAbstract:A utilização de plantas inseticidas é cada vez mais frequente no controle de pragas. As plantas podem produzir diferentes compostos com atividade inseticida. Os óleos essenciais destacam-se dentre estes compostos, possuindo diferentes modos de ação inseticida, podendo ser uma ferramenta útil no manejo integrado de pragas. O objetivo deste trabalho é avaliar o efeito de óleos essenciais cítricos e seu composto majoritário D-limonemo visando sua adoção como métodos alternativos de controle de Agrotis Ipsilon (Hufnagel) (Lepidoptera: Noctuidae). Para a realização do experimento foram utilizadas 50 lagartas de 1º ínstar por tratamento. Foram aplicados os óleos essenciais com auxilio do aerógrafo, sendo utilizado na concentração de 10% v v-1 para os óleos cítricos e o D-limoneno e para testemunha foi pulverizado água destilada. A avaliação foi realizada após 24, 48 e 72 horas verificando o número de lagartas mortas. Não foi observado efeito tóxico dos óleos essenciais cítricos e do D-limoneno sobre as lagartas de A. Ipsilon. Deste modo, os óleos essenciais testados não são eficazes no controle da praga