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Víctor M. Jiménez – One of the best experts on this subject based on the ideXlab platform.

  • In vitro propagation of bamboo species through axillary shoot proliferation: a review
    Plant Cell Tissue and Organ Culture (PCTOC), 2017
    Co-Authors: Manpreet Sandhu, Shabir H. Wani, Víctor M. Jiménez

    Abstract:

    Bamboos, very relevant plants in many countries around the world, are propagated at large scale with extreme difficulties. Use of seeds is challenging because of plant’s sporadic flowering and long flowering cycles, together with seed recalcitrance and consumption by wild animals. Vegetative propagation of bamboo is mainly conducted by cuttings and by Air Layering. However, these methods are only useful at small-scale because they damage the mother plants, propagation material is bulky and difficult to be transported and is only available during few months of the year. Therefore, in vitro propagation offers the opportunity to obtain large progenies from elite genotypes. In most cases, when developing protocols for in vitro propagation of plants, specific conditions for individual species, genotypes and even development stages of the donor plants must be identified by trial-and-error experiments. Because of the size of and the large diversity observed in this plant family, it usually takes several months to define most adequate culture medium, combination of plant growth regulators and of other compounds for fostering the desired development in the explants. Therefore, in this detailed review, that also puts together results from hard-to-find literature, we list all identified cases, in which development of axillary shoots was used to propagate bamboo plants, by presenting successful ways for disinfection, in vitro bud sprouting, multiplication, rooting and acclimatization.

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Manpreet Sandhu – One of the best experts on this subject based on the ideXlab platform.

  • In vitro propagation of bamboo species through axillary shoot proliferation: a review
    Plant Cell Tissue and Organ Culture (PCTOC), 2017
    Co-Authors: Manpreet Sandhu, Shabir H. Wani, Víctor M. Jiménez

    Abstract:

    Bamboos, very relevant plants in many countries around the world, are propagated at large scale with extreme difficulties. Use of seeds is challenging because of plant’s sporadic flowering and long flowering cycles, together with seed recalcitrance and consumption by wild animals. Vegetative propagation of bamboo is mainly conducted by cuttings and by Air Layering. However, these methods are only useful at small-scale because they damage the mother plants, propagation material is bulky and difficult to be transported and is only available during few months of the year. Therefore, in vitro propagation offers the opportunity to obtain large progenies from elite genotypes. In most cases, when developing protocols for in vitro propagation of plants, specific conditions for individual species, genotypes and even development stages of the donor plants must be identified by trial-and-error experiments. Because of the size of and the large diversity observed in this plant family, it usually takes several months to define most adequate culture medium, combination of plant growth regulators and of other compounds for fostering the desired development in the explants. Therefore, in this detailed review, that also puts together results from hard-to-find literature, we list all identified cases, in which development of axillary shoots was used to propagate bamboo plants, by presenting successful ways for disinfection, in vitro bud sprouting, multiplication, rooting and acclimatization.

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Wagner Campos Otoni – One of the best experts on this subject based on the ideXlab platform.

  • resgate vegetativo por alporquia de genotipos adultos de urucum bixa orellana l
    Ciencia Florestal, 2010
    Co-Authors: Nilton Cesar Mantovani, Magali Ferrari Grando, Aloisio Xavier, Wagner Campos Otoni

    Abstract:

    This study aimed to evaluate the usefulness of an Air Layering technique for vegetative rescue of annatto ( Bixa orellana L.) genotypes and obtainment of propagules for vegetative propagation. Ten 12 year-old annatto stock plants, whose genotypes are derived from artificial crossing between “HAiry-green capsule” X “Smooth-red capsule” were used in this study. Air Layering adventitious roots were induced in branches (1-2 cm diameter) using as substrate a mixture of vermiculite and moss. The effects of (1) the type of girdling (total or partial), 1 cm in length, (2) IBA (indole-3-butyric acid at 0 and 4.92 mM) solution in paper filter and (3) the covering with either transparent or dark plastic films and tencel were evaluated. The technique of Air Layering provided rooted branches for the 10 genotypes evaluated, with efficiency ranging from 20 to 100%, and a genotype effect was observed on rooting frequency. Under greenhouse conditions, the survival of layers derived from totally girdled stems treated with IBA and protected with transparent plastic was 100%. Established layers provided vigorous branching from which shoot apexes or nodal segment explants were obtained for in vitro establishment and propagation of this species.

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  • resgate vegetativo por alporquia de genotipos adultos de urucum bixa orellana l vegetative rescue of adult genotypes of annatto bixa orellana l by Air Layering
    , 2010
    Co-Authors: Nilton Cesar Mantovani, Magali Ferrari Grando, Aloisio Xavier, Wagner Campos Otoni

    Abstract:

    This study aimed to evaluate the usefulness of an Air Layering technique for vegetative rescue of annatto (Bixa orellana L.) genotypes and obtainment of propagules for vegetative propagation. Ten 12 year-old annatto stock plants, whose genotypes are derived from artificial crossing between “HAiry-green capsule” X “Smooth-red capsule” were used in this study. Air Layering adventitious roots were induced in branches (1-2 cm diameter) using as substrate a mixture of vermiculite and moss. The effects of (1) the type of girdling (total or partial), 1 cm in length, (2) IBA (indole-3-butyric acid at 0 and 4.92 mM) solution in paper filter and (3) the covering with either transparent or dark plastic films and tencel were evaluated. The technique of Air Layering provided rooted branches for the 10 genotypes evaluated, with efficiency ranging from 20 to 100%, and a genotype effect was observed on rooting frequency. Under greenhouse conditions, the survival of layers derived from totally girdled stems treated with IBA and protected with transparent plastic was

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