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Philip H S Jen - One of the best experts on this subject based on the ideXlab platform.
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focal electrical stimulation of dorsal nucleus of the lateral lemniscus modulates auditory response properties of inferior collicular neurons in the Albino Mouse
Hearing Research, 2019Co-Authors: Xin Wang, Dandan Yang, Yanling Cheng, Philip H S Jen, Cuihong Yang, Qicai ChenAbstract:Abstract The inferior colliculus (IC) receives and integrates excitatory and inhibitory inputs from many bilateral lower auditory nuclei, intrinsic projections within IC, contralateral IC through the commissure of IC and from the auditory cortex (AC). These excitatory and inhibitory inputs from both ascending and descending auditory pathways contribute significantly to auditory response properties and temporal signal processing in IC. The present study examines the contribution of gamma-aminobutyric acid-ergic (GABAergic) inhibition of dorsal nucleus of the lateral lemniscus (DNLL) in influencing the response properties and amplitude sensitivity of contralateral IC neurons using focal electrical stimulation of contralateral DNLL and by the application of bicuculline to the recording site of modulated IC neurons. Focal electrical stimulation of contralateral DNLL produces inhibition (78.1%), facilitation (7.1%) or no effect (14.8%) in the number of spikes, firing duration and the first-spike latency of modulated IC neurons. The degree of modulation is inversely correlated to the difference in best frequency (BF) between electrically stimulated DNLL neurons and modulated IC neurons (p
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the role of the dorsal nucleus of the lateral lemniscus in shaping the auditory response properties of the central nucleus of the inferior collicular neurons in the Albino Mouse
Neuroscience, 2018Co-Authors: Huimei Wang, Xin Wang, Philip H S Jen, Shuang Shen, Tihua Zheng, Ying YangAbstract:Abstract In the ascending auditory pathway, the central nucleus of the inferior colliculus (IC) receives and integrates excitatory and inhibitory inputs from many bilateral lower auditory nuclei, intrinsic projections within the IC, contralateral IC through the commissure of the IC and from the auditory cortex. All these presynaptic excitatory and inhibitory inputs dynamically shape and modulate the auditory response properties of individual IC neurons. For this reason, acoustic response properties vary among individual IC neurons due to different activity pattern of presynaptic inputs. The present study examines modulation of auditory response properties of IC neurons by combining sound stimulation with focal electrical stimulation of the contralateral dorsal nucleus of the lateral lemniscus (referred to as ESDNLL) in the Albino Mouse. Brief ESDNLL produces variation (increase or decrease) in the number of impulses, response latency and discharge duration of modulated IC neurons. Additionally, 30-minute short-term ESDNLL alone produces variation in the best frequency (BF) and minimum threshold (MT) of modulated IC neurons. These varied response parameters recover in different manner and time course among individual modulated IC neurons. Possible pathways and neural mechanisms underlying these findings are discussed.
Mohssen Morowati - One of the best experts on this subject based on the ideXlab platform.
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inhalation toxicity studies of thimet phorate in the male swiss Albino Mouse mus musculus ii lung histopathology pseudocholinesterase level and haematological studies
Environmental Pollution, 1998Co-Authors: Mohssen MorowatiAbstract:Abstract Sub-chronic exposure of the male swiss Albino Mouse, Mus musculus to the recommended field dose of Thimet (Phorate; 6728.5 mg m −3 ) in a whole-body inhalation chamber was studied on the 2nd, 4th, 6th, 8th, 10th and 12th week of exposure. A significant depression of pseudocholinesterase (Che) level was observed throughout the experimental period. A significant decrease in haemoglobin concentration (Hb conc.), red blood corpuscle count (RBC) count and packed cell volume percent (PCV%) was observed from the 6th week of exposure until the end of the experiment. Total leucocyte count (WBC count) showed a significant rise in the 2nd week of exposure (this rise was due to the rise in the monocyte and neutrophil counts), and later from the 6th week onwards showed a significant decrease until the end of the experiment (the decrease was mainly due to the fall in the lymphocyte count). The lung in the exposed animals showed various degrees of bronchopneumonia, emphysema and collapse throughout the experiment. After a 30 day recovery period almost all the blood parameters were back to the normal levels, except the total leucocyte count which showed a higher level than the normal count. However, the lung in the recovered animals showed emphysematous changes but no bronchopneumonia and inflammation. The results suggest that inhalation of the pesticide, Thimet could cause damages to Mus musculus , it also suggest that the improper handling and usage of this pesticide by human beings could affect their well being and efficiency at work.
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inhalation toxicity studies of thimet phorate in male swiss Albino Mouse mus musculus i hepatotoxicity
Environmental Pollution, 1997Co-Authors: Mohssen MorowatiAbstract:Biochemical and histopathological changes in serum and liver of the male Swiss Albino Mouse, Mus musculus, exposed subchronically to the recommended field dose of Thimet (6728.5 mg m−3) in a whole body inhalation chamber were studied in the second, fourth, sixth, eighth, tenth and twelfth week of exposure. A significant rise in Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) activities were observed throughout the experiment. Alkaline phosphatase (ALP) activity showed a significant rise from the fourth week of exposure until the end of the experiment. The rise in the activities of these enzymes suggested hepatocellular necroinflammatory disease. Total bilirubin level was increased significantly throughout the experiment (indicative of jaundice); however the direct bilirubin level was significantly high in the tenth and twelfth week of exposure and indirect bilirubin showed a significant rise from the fourth week of exposure until the end of the experiment. Changes in bilirubin levels (direct and indirect) suggested both prehepatic and hepatocellular hyperbilirubinaemia. The biochemical changes observed and fatty change in the liver were confirmed by histopathological studies. Liver lesions were present throughout the experimental period. These consisted of mild to severe multifocal cloudy, hydropic and fatty degenerations, with necrosis. After a 30-day recovery period most of the histopathological and biochemical changes except the AST level were almost back to normal. These results suggest the inhalation of Thimet (Phorate) at the recommended field dose could affect the liver of Mus musculus.
Xin Wang - One of the best experts on this subject based on the ideXlab platform.
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focal electrical stimulation of dorsal nucleus of the lateral lemniscus modulates auditory response properties of inferior collicular neurons in the Albino Mouse
Hearing Research, 2019Co-Authors: Xin Wang, Dandan Yang, Yanling Cheng, Philip H S Jen, Cuihong Yang, Qicai ChenAbstract:Abstract The inferior colliculus (IC) receives and integrates excitatory and inhibitory inputs from many bilateral lower auditory nuclei, intrinsic projections within IC, contralateral IC through the commissure of IC and from the auditory cortex (AC). These excitatory and inhibitory inputs from both ascending and descending auditory pathways contribute significantly to auditory response properties and temporal signal processing in IC. The present study examines the contribution of gamma-aminobutyric acid-ergic (GABAergic) inhibition of dorsal nucleus of the lateral lemniscus (DNLL) in influencing the response properties and amplitude sensitivity of contralateral IC neurons using focal electrical stimulation of contralateral DNLL and by the application of bicuculline to the recording site of modulated IC neurons. Focal electrical stimulation of contralateral DNLL produces inhibition (78.1%), facilitation (7.1%) or no effect (14.8%) in the number of spikes, firing duration and the first-spike latency of modulated IC neurons. The degree of modulation is inversely correlated to the difference in best frequency (BF) between electrically stimulated DNLL neurons and modulated IC neurons (p
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the role of the dorsal nucleus of the lateral lemniscus in shaping the auditory response properties of the central nucleus of the inferior collicular neurons in the Albino Mouse
Neuroscience, 2018Co-Authors: Huimei Wang, Xin Wang, Philip H S Jen, Shuang Shen, Tihua Zheng, Ying YangAbstract:Abstract In the ascending auditory pathway, the central nucleus of the inferior colliculus (IC) receives and integrates excitatory and inhibitory inputs from many bilateral lower auditory nuclei, intrinsic projections within the IC, contralateral IC through the commissure of the IC and from the auditory cortex. All these presynaptic excitatory and inhibitory inputs dynamically shape and modulate the auditory response properties of individual IC neurons. For this reason, acoustic response properties vary among individual IC neurons due to different activity pattern of presynaptic inputs. The present study examines modulation of auditory response properties of IC neurons by combining sound stimulation with focal electrical stimulation of the contralateral dorsal nucleus of the lateral lemniscus (referred to as ESDNLL) in the Albino Mouse. Brief ESDNLL produces variation (increase or decrease) in the number of impulses, response latency and discharge duration of modulated IC neurons. Additionally, 30-minute short-term ESDNLL alone produces variation in the best frequency (BF) and minimum threshold (MT) of modulated IC neurons. These varied response parameters recover in different manner and time course among individual modulated IC neurons. Possible pathways and neural mechanisms underlying these findings are discussed.
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response characteristics of neurons to tone in dorsal nucleus of the lateral lemniscus of the Mouse
Acta physiologica Sinica, 2016Co-Authors: Yanling Cheng, Dandan Yang, Xin WangAbstract:The dorsal nucleus of lateral lemniscus (DNLL) is a nucleus in the auditory ascending pathway, and casts inhibitory efferent projections to the inferior colliculus. Studies on the DNLL are less than studies on the auditory brain stem and inferior colliculus. To date, there is no information about response characteristics of neurons in DNLL of Albino Mouse. Under free field conditions, we used extracellular single unit recording to study the acoustic signal characteristics of DNLL neurons in Kunming mice (Mus musculus). Transient (36%) and ongoing (64%) firing patterns were found in 96 DNLL neurons. Neurons with different firing patterns have significant differences in characteristic frequency and minimal threshold. We recorded frequency tuning curves (FTCs) of 87 DNLL neurons. All of the FTCs exhibit an open "V" shape. There is no significant difference in FTCs between transient and ongoing neurons, but among the ongoing neurons, the FTCs of sustained neurons are sharper than those of onset plus sustained neurons and pauser neurons. Our results showed that the characteristic frequency of DNLL neurons of mice was not correlated with depth, supporting the view that the DNLL of Mouse has no frequency topological organization through dorsal-ventral plane, which is different from cats and some other animals. Furthermore, by using rate-intensity function (RIF) analysis the Mouse DNLL neurons can be classified as monotonic (60%), saturated (31%) and non-monotonic (8%) types. Each RIF type includes transient and ongoing firing patterns. Dynamic range of the transient firing pattern is smaller than that of ongoing firing ones (P < 0.01), suggesting that the inhibitory inputs may underlie the formation of transient firing pattern. Multiple firing patterns and intensity coding of DNLL neurons may derive from the projections from multiple auditory nuclei, and play different roles in auditory information processing.
Alfonso Barbarisi - One of the best experts on this subject based on the ideXlab platform.
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Research Article Vitronectin Absorbed on Nanoparticles Mediate Cell Viability/Proliferation and Uptake by 3T3 Swiss AlbinoMouse Fibroblasts: In Vitro Study
2016Co-Authors: Manlio Barbarisi, Alfonso BarbarisiAbstract:Copyright © 2013 F. Rosso et al.is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. �e study the interaction of 3T3 Swiss Albino Mouse �broblasts with polymeric nanoparticles (NPs) and investigate cellular behaviour in terms of viability/cytotoxicity, cell cycle, NPs uptake, MAP kinase (ERK1/2), and focal adhesion kinase (FAK) activation. Aer incubation of NPs with cell culture media, western blot analysis showed that Vitronectin is retained by NPs, while Fibronectin is not detected. From cytotoxicity studies (MTT and BrdU methods) an LD50 of about 1.5mg/mL results for NPs. However, NPs in the range 0.01–0.30mg/mL are able to trigger a statistically signi�cant increase in proliferation and cell cycle progression in dose and time depending manner. Also, biochemical evaluation of ERK1/2 and FAK clearly shows an increasing phosphorylation in a dose and time depending manner. Finally, we found by transmission electron microscopy that NPs are internalised by cells. Competitively blocking VN-integrin receptors with echistatin (
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Vitronectin Absorbed on Nanoparticles Mediate Cell Viability/Proliferation and Uptake by 3T3 Swiss Albino Mouse Fibroblasts: In Vitro Study
Hindawi Limited, 2013Co-Authors: F. Rosso, Gennaro Cafiero, Manlio Barbarisi, G. Marino, A. Grimaldi, E. Chiellini, F. Chiellini, Alfonso BarbarisiAbstract:We study the interaction of 3T3 Swiss Albino Mouse fibroblasts with polymeric nanoparticles (NPs) and investigate cellular behaviour in terms of viability/cytotoxicity, cell cycle, NPs uptake, MAP kinase (ERK1/2), and focal adhesion kinase (FAK) activation. After incubation of NPs with cell culture media, western blot analysis showed that Vitronectin is retained by NPs, while Fibronectin is not detected. From cytotoxicity studies (MTT and BrdU methods) an LD50 of about 1.5 mg/mL results for NPs. However, NPs in the range 0.01–0.30 mg/mL are able to trigger a statistically significant increase in proliferation and cell cycle progression in dose and time depending manner. Also, biochemical evaluation of ERK1/2 and FAK clearly shows an increasing phosphorylation in a dose and time depending manner. Finally, we found by transmission electron microscopy that NPs are internalised by cells. Competitively blocking VN-integrin receptors with echistatin (1 μg/mL) results in a decrease of viability/proliferation, cell cycle progression, cellular uptake, and FAK/ERK activation showing the involvement of Vitronectin receptors in signal transduction. In conclusion, our results show that cell surface NPs interactions are mediated by absorbed plasma proteins (i.e., Vitronectin) that represent an external stimuli, switched to the nucleus by FAK enzyme, which in turn modulate fibroblasts viability/proliferation
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cell surface protein detection with immunogold labelling in esem optimisation of the method and semi quantitative analysis
Journal of Cellular Physiology, 2008Co-Authors: Livio Muscariello, Gennaro Cafiero, Francesco Rosso, Manlio Barbarisi, Gerardo Marino, Alfonso BarbarisiAbstract:In this work we used a combination of immunogold labelling (IGL) and environmental scanning electron microscopy (ESEM) to detect the presence of a protein on the cell surface. To achieve this purpose we chose as experimental system 3T3 Swiss Albino Mouse Fibroblasts and galectin-3. This protein, whose sub-cellular distribution is still under discussion, is involved in a large number of cell physiological and pathological processes. IGL technique has been utilised by many authors in combination with SEM and TEM to obtain the identification/localisation of receptors and antigens, both in cells and tissues. ESEM represents an important tool in biomedical research, since it does not require any severe processing of the sample, lowering the risk of generating artefacts and interfere with IGL procedure. The absence of metal coating could yield further advantages for our purpose as the labelling detection is based on the atomic number difference between Nanogold spheres and the biological material. Using the gaseous secondary electron detector (GSED) compositional contrast is easily revealed by the backscattered electrons component of the signal. In spite of this fact, only few published papers present a combination of ESEM and IGL. Hereby we present our method, optimised to improve the intensity and the specificity of the labelling signal, in order to obtain a semi-quantitative evaluation of the labelling signal. J. Cell. Physiol. 214: 769–776, 2008. © 2007 Wiley-Liss, Inc.
Qicai Chen - One of the best experts on this subject based on the ideXlab platform.
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focal electrical stimulation of dorsal nucleus of the lateral lemniscus modulates auditory response properties of inferior collicular neurons in the Albino Mouse
Hearing Research, 2019Co-Authors: Xin Wang, Dandan Yang, Yanling Cheng, Philip H S Jen, Cuihong Yang, Qicai ChenAbstract:Abstract The inferior colliculus (IC) receives and integrates excitatory and inhibitory inputs from many bilateral lower auditory nuclei, intrinsic projections within IC, contralateral IC through the commissure of IC and from the auditory cortex (AC). These excitatory and inhibitory inputs from both ascending and descending auditory pathways contribute significantly to auditory response properties and temporal signal processing in IC. The present study examines the contribution of gamma-aminobutyric acid-ergic (GABAergic) inhibition of dorsal nucleus of the lateral lemniscus (DNLL) in influencing the response properties and amplitude sensitivity of contralateral IC neurons using focal electrical stimulation of contralateral DNLL and by the application of bicuculline to the recording site of modulated IC neurons. Focal electrical stimulation of contralateral DNLL produces inhibition (78.1%), facilitation (7.1%) or no effect (14.8%) in the number of spikes, firing duration and the first-spike latency of modulated IC neurons. The degree of modulation is inversely correlated to the difference in best frequency (BF) between electrically stimulated DNLL neurons and modulated IC neurons (p