The Experts below are selected from a list of 13617 Experts worldwide ranked by ideXlab platform
Shulin Chen - One of the best experts on this subject based on the ideXlab platform.
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bicarbonate based integrated carbon capture and Algae production system with alkalihalophilic cyanobacterium
Bioresource Technology, 2013Co-Authors: Farah C Elloy, Yubin Zheng, Yucai Hu, Shulin ChenAbstract:An extremely alkalihalophilic cyanobacteria Euhalothece ZM001 was tested in the Bicarbonate-based Integrated Carbon Capture and Algae Production System (BICCAPS), which utilize bicarbonate as carbon source for Algae Culture and use the regenerated carbonate to absorb CO2. Culture conditions including temperature, inoculation rate, medium composition, pH, and light intensity were investigated. A final biomass concentration of 4.79 g/L was reached in tissue flask Culture with 1.0 M NaHCO3/Na2CO3. The biomass productivity of 1.21 g/L/day was achieved under optimal conditions. When pH increased from 9.55 to 10.51, 0.256 M of inorganic carbon was consumed during the Culture process. This indicated sufficient carbon can be supplied as bicarbonate to the Culture. This study proved that a high biomass production rate can be achieved in a BICCAPS. This strategy can also lead to new design of photobioreactors that provides an alternative supply of CO2 to sparging.
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two stage heterotrophic and phototrophic Culture strategy for algal biomass and lipid production
Bioresource Technology, 2012Co-Authors: Yubin Zheng, Zhanyou Chi, Ben F Lucker, Shulin ChenAbstract:A two-stage heterotrophic and phototrophic Culture strategy for algal biomass and lipid production was studied, wherein high density heterotrophic Cultures of Chlorella sorokiniana serve as seed for subsequent phototrophic growth. The data showed growth rate, cell density and productivity of heterotrophic C. sorokiniana were 3.0, 3.3 and 7.4 times higher than phototrophic counterpart, respectively. Hetero- and phototrophic algal seeds had similar biomass/lipid production and fatty acid profile when inoculated into phototrophic Culture system. To expand the application, food waste and wastewater were tested as feedstock for heterotrophic growth, and supported cell growth successfully. These results demonstrated the advantages of using heterotrophic Algae cells as seeds for open Algae Culture system. Additionally, high inoculation rate of heterotrophic algal seed can be utilized as an effective method for contamination control. This two-stage heterotrophic phototrophic process is promising to provide a more efficient way for large scale production of algal biomass and biofuels.
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bicarbonate produced from carbon capture for Algae Culture
Trends in Biotechnology, 2011Co-Authors: Zhanyou Chi, James V Ofallon, Shulin ChenAbstract:Using captured CO 2 to grow microAlgae is limited by the high cost of CO 2 capture and transportation, as well as significant CO 2 loss during Algae Culture. Moreover, Algae grow poorly at night, but CO 2 cannot be temporarily stored until sunrise. To address these challenges, we discuss a process where CO 2 is captured as bicarbonate and used as feedstock for Algae Culture, and the carbonate regenerated by the Culture process is used as an absorbent to capture more CO 2 . This process would significantly reduce carbon capture costs because it does not require additional energy for carbonate regeneration. Furthermore, not only would transport of the aqueous bicarbonate solution cost less than for that of compressed CO 2 , but using bicarbonate would also provide a superior alternative for CO 2 delivery to an Algae Culture system.
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lipid production by culturing oleaginous yeast and Algae with food waste and municipal wastewater in an integrated process
Applied Biochemistry and Biotechnology, 2011Co-Authors: Zhanyou Chi, Yubin Zheng, Anping Jiang, Shulin ChenAbstract:Food waste and municipal wastewater are promising feedstocks for microbial lipid biofuel production, and corresponding production process is to be developed. In this study, different oleaginous yeast strains were tested to grow in hydrolyzed food waste, and growths of Cryptococcus curvatus, Yarrowia lipolytica, and Rhodotorula glutinis in this condition were at same level as in glucose Culture as control. These strains were further tested to grow in municipal primary wastewater. C. curvatus and R. glutinis had higher production than Y. lipolytica in media made from primary wastewater, both with and without glucose supplemented. Finally, a process was tested to grow C. curvatus and R. glutinis in media made from food waste and municipal wastewater, and the effluents from these processes were further treated with yeast Culture and phototrophic Algae Culture; 1.1 g/L C. curvatus and 1.5 g/L R. glutinis biomass were further produced in second-step yeast Cultures, as well as 1.53 and 0.58 g/L Chlorella sorokiniana biomass in phototrophic Cultures. The residual nitrogen concentrations in final effluents were 33 mg/L and 34 mg/L, respectively, and the residual phosphorus concentrations were 1.5 and 0.6 mg/L, respectively. The lipid contents in the produced biomass were from 18.7% to 28.6%.
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production of ω 3 polyunsaturated fatty acids from cull potato using an Algae Culture process
Applied Biochemistry and Biotechnology, 2007Co-Authors: Zhanyou Chi, Yan Liu, Craig Frear, Zhiyou Wen, Shulin ChenAbstract:Algal cultivation for converting cull potato to docosahexaenoic acid (DHA) was studied. Schizochytrium limacinum SR21 was selected as the better producing strain, compared with Thraustochytrium aureum because of higher cell density and DHA content. Used as both carbon and nitrogen source, an optimal ratio of hydrolyzed potato broth in the Culture medium was determined as 50%, with which the highest production of 21.7 g/L dry Algae biomass and 5.35 g/L DHA was obtained, with extra glucose supplemented. Repeat Culture further improved the cell density but not fed batch Culture, suggesting limited growth was most likely caused by metabolites inhibition.
Dieter G Muller - One of the best experts on this subject based on the ideXlab platform.
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pathogens of brown Algae Culture studies of anisolpidium ectocarpii and a rosenvingei reveal that the anisolpidiales are uniflagellated oomycetes
European Journal of Phycology, 2017Co-Authors: Claire M M Gachon, Martina Strittmatter, Yacine Badis, Kyle I Fletcher, Pieter Van West, Dieter G MullerAbstract:ABSTRACTUsing laboratory Cultures, we have documented the life cycle of Anisolpidium ectocarpii, a pathogen of Ectocarpus and other filamentous brown Algae, and presented preliminary observations on Anisolpidium rosenvingei, a pathogen of Pylaiella littoralis. Consistent with earlier reports, the zoospores of both species have a single anterior flagellum, which justified the placement of Anisolpidium amongst the Hyphochytriales (Hyphochytridiomycota). We have also shown that A. ectocarpii can complete its infection cycle in a broad selection of species from various brown algal orders, whereas A. rosenvingei seemingly exhibits a strict specificity for unilocular sporangia of P. littoralis. Unexpectedly, nuclear (18S rRNA) and mitochondrial (cox1, cox2) markers regroup A. ectocarpii and A. rosenvingei, into a hitherto unrecognized monophyletic clade within the oomycetes (Oomycota), most closely related to the Olpidiopsidales. The Anisolpidium genus is therefore entirely distinct from the Hyphochytridiomycot...
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pathogens of brown Algae Culture studies of anisolpidium ectocarpii and a rosenvingei reveal that the anisolpidiales are uniflagellated oomycetes
European Journal of Phycology, 2017Co-Authors: Claire M M Gachon, Martina Strittmatter, Yacine Badis, Kyle I Fletcher, Pieter Van West, Dieter G MullerAbstract:Using laboratory Cultures, we have documented the life cycle of Anisolpidium ectocarpii, a pathogen of Ectocarpus and other filamentous brown Algae, and presented preliminary observations on Anisol...
Paul C Southgate - One of the best experts on this subject based on the ideXlab platform.
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successful large scale hatchery Culture of sandfish holothuria scabra using micro Algae concentrates as a larval food source
Aquaculture Reports, 2018Co-Authors: Thane A Militz, Esther Leini, Nguyen Dinh Quang Duy, Paul C SouthgateAbstract:Abstract This paper reports methodology for large-scale hatchery Culture of sandfish, Holothuria scabra, in the absence of live, Cultured micro-Algae. We demonstrate how commercially-available micro-Algae concentrates can be incorporated into hatchery protocols as the sole larval food source to completely replace live, Cultured micro-Algae. Micro-Algae concentrates supported comparable hatchery production of sandfish to that of live, Cultured micro-Algae traditionally used in large-scale hatchery Culture. The hatchery protocol presented allowed a single technician to achieve production of more than 18,800 juvenile sandfish at 40 days post-fertilisation in a low-resource hatchery in Papua New Guinea. Growth of auricularia larvae fed micro-Algae concentrates was represented by the equation length (μm) = 307.8 × ln( day ) + 209.2 (R 2 = 0.93) while survival over the entire 40 day hatchery cycle was described by the equation survival = 2 × day −1.06 (R 2 = 0.74). These results show that micro-Algae concentrates have great potential for simplifying hatchery Culture of sea cucumbers by reducing infrastructural and technical resources required for live micro-Algae Culture. The hatchery methodology described in this study is likely to have applicability to low-resource hatcheries throughout the Indo-Pacific and could support regional expansion of sandfish hatchery production.
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hatchery Culture of the winged pearl oyster pteria penguin without living micro Algae
Aquaculture, 2016Co-Authors: Paul C Southgate, Andrew C Beer, Poasi NgaluafeAbstract:Abstract This paper reports on successful hatchery production of the winged pearl oyster, Pteria penguin, without the use of live micro-Algae. Larval nutrition was provided by commercially available micro-Algae concentrates, Instant Algae® (“Isochrysis 1800®” and “Pavlova 1800®”, Reed MariCulture Inc., San Jose, CA, USA). Larvae were first transferred to settlement tanks on day 17 when their mean antero-posterior measurement (APM) was 240.2 ± 8.6 μm. Approximately 6.4% of larvae survived to day 17 and more than 700,000 eyed pediveligers were transferred to settlement tanks between day 17 and day 25. Approximately 33,000 spat were harvested from spat collectors on day 105, representing a survival rate of 4.7% from the eyed pediveliger stage. Growth and development of larvae in this study were superior to those reported in a prior study that used a ternary live micro-Algae diet to feed P. penguin larvae. Our results indicate that the products used in this study proved nutritious for P. penguin larvae and supported normal growth and development through settlement. The use of commercially available micro-Algae concentrates as a replacement for live micro-Algae in pearl oyster hatcheries supports development of simplified larval rearing protocols, without live micro-Algae Culture, that are more appropriate to Pacific island nations. Statement of relevance This paper reports for the first time on successful hatchery production of pearl oysters without the use of live micro-Algae. Successful replacement of live micro-Algae with commercially available micro-Algae concentrates as a larval food source supports development of simpler, cheaper hatchery facilities, and larval rearing protocols that are more appropriate to Pacific island nations.
Poasi Ngaluafe - One of the best experts on this subject based on the ideXlab platform.
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hatchery Culture of the winged pearl oyster pteria penguin without living micro Algae
Aquaculture, 2016Co-Authors: Paul C Southgate, Andrew C Beer, Poasi NgaluafeAbstract:Abstract This paper reports on successful hatchery production of the winged pearl oyster, Pteria penguin, without the use of live micro-Algae. Larval nutrition was provided by commercially available micro-Algae concentrates, Instant Algae® (“Isochrysis 1800®” and “Pavlova 1800®”, Reed MariCulture Inc., San Jose, CA, USA). Larvae were first transferred to settlement tanks on day 17 when their mean antero-posterior measurement (APM) was 240.2 ± 8.6 μm. Approximately 6.4% of larvae survived to day 17 and more than 700,000 eyed pediveligers were transferred to settlement tanks between day 17 and day 25. Approximately 33,000 spat were harvested from spat collectors on day 105, representing a survival rate of 4.7% from the eyed pediveliger stage. Growth and development of larvae in this study were superior to those reported in a prior study that used a ternary live micro-Algae diet to feed P. penguin larvae. Our results indicate that the products used in this study proved nutritious for P. penguin larvae and supported normal growth and development through settlement. The use of commercially available micro-Algae concentrates as a replacement for live micro-Algae in pearl oyster hatcheries supports development of simplified larval rearing protocols, without live micro-Algae Culture, that are more appropriate to Pacific island nations. Statement of relevance This paper reports for the first time on successful hatchery production of pearl oysters without the use of live micro-Algae. Successful replacement of live micro-Algae with commercially available micro-Algae concentrates as a larval food source supports development of simpler, cheaper hatchery facilities, and larval rearing protocols that are more appropriate to Pacific island nations.
Claire M M Gachon - One of the best experts on this subject based on the ideXlab platform.
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pathogens of brown Algae Culture studies of anisolpidium ectocarpii and a rosenvingei reveal that the anisolpidiales are uniflagellated oomycetes
European Journal of Phycology, 2017Co-Authors: Claire M M Gachon, Martina Strittmatter, Yacine Badis, Kyle I Fletcher, Pieter Van West, Dieter G MullerAbstract:ABSTRACTUsing laboratory Cultures, we have documented the life cycle of Anisolpidium ectocarpii, a pathogen of Ectocarpus and other filamentous brown Algae, and presented preliminary observations on Anisolpidium rosenvingei, a pathogen of Pylaiella littoralis. Consistent with earlier reports, the zoospores of both species have a single anterior flagellum, which justified the placement of Anisolpidium amongst the Hyphochytriales (Hyphochytridiomycota). We have also shown that A. ectocarpii can complete its infection cycle in a broad selection of species from various brown algal orders, whereas A. rosenvingei seemingly exhibits a strict specificity for unilocular sporangia of P. littoralis. Unexpectedly, nuclear (18S rRNA) and mitochondrial (cox1, cox2) markers regroup A. ectocarpii and A. rosenvingei, into a hitherto unrecognized monophyletic clade within the oomycetes (Oomycota), most closely related to the Olpidiopsidales. The Anisolpidium genus is therefore entirely distinct from the Hyphochytridiomycot...
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pathogens of brown Algae Culture studies of anisolpidium ectocarpii and a rosenvingei reveal that the anisolpidiales are uniflagellated oomycetes
European Journal of Phycology, 2017Co-Authors: Claire M M Gachon, Martina Strittmatter, Yacine Badis, Kyle I Fletcher, Pieter Van West, Dieter G MullerAbstract:Using laboratory Cultures, we have documented the life cycle of Anisolpidium ectocarpii, a pathogen of Ectocarpus and other filamentous brown Algae, and presented preliminary observations on Anisol...