The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
M S Agren - One of the best experts on this subject based on the ideXlab platform.
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a fibre free Alginate Dressing in the treatment of split thickness skin graft donor sites
Journal of The European Academy of Dermatology and Venereology, 1998Co-Authors: H H Steenfos, M S AgrenAbstract:Background Alginate Dressings are gaining acceptance in wound management although comparative published data with conventional treatment is inconclusive. Aims The aim of this randomised controlled study was to compare a fibre-free Alginate Dressing (Comfeel SeaSorb) with conventional treatment of standardised split-thickness skin graft donor sites in 17 patients regarding initial absorption of blood and healing. Results The Alginate Dressing absorbed 40% (P < 0.05) more blood, measured as total iron content of used Dressings, during the first 10 post-wounding minutes than fine mesh gauze, resulting in less subsequent bleeding. Light microscopic examination of punch biopsies obtained from 10 wounds on post-operative day 6 demonstrated that nine wounds treated with the Alginate Dressing compared with seven wounds treated conventionally with paraffin-impregnated gauze (Jelonet8) were completely epithelialised, a statistically non-significant difference (P = 0.46). Conclusions In conclusion, the fibre-free Alginate Dressing showed increased initial blood absorption resulting in quicker haemostasis but showed no greater beneficial effect on epithelialisation of split-thickness skin graft donor sites compared with conventional topical treatment. 0 1998 Elsevier Science B.V. All rights reserved
Steven L Percival - One of the best experts on this subject based on the ideXlab platform.
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the visualisation and speed of kill of wound isolates on a silver Alginate Dressing
International Wound Journal, 2012Co-Authors: Samuel James Hooper, Steven L Percival, Katja E Hill, D W P Thomas, Anthony Joseph Hayes, David Wynne WilliamsAbstract:In chronic wound management, Alginate Dressings are used to absorb exudate and reduce the microbial burden. Silver Alginate offers the added benefit of an additional antimicrobial pressure on contaminating microorganisms. This present study compares the antimicrobial activity of a RESTORE silver Alginate Dressing with a silver-free control Dressing using a combination of in vitro culture and imaging techniques. The wound pathogens examined included Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, β-haemolytic Streptococcus, and strictly anaerobic bacteria. The antimicrobial efficacy of the Dressings was assessed using log10 reduction and 13-day corrected zone of inhibition (CZOI) time-course assays. Confocal laser scanning microscopy (CLSM) was used to visualise the relative proportions of live/dead microorganisms sequestered into the Dressings over 24 hours and estimate the comparative speed of kill. The RESTORE silver Alginate Dressing showed significantly greater log10 reductions and CZOIs for all microorganisms compared with the control, indicating the antimicrobial effect of ionic silver. Antimicrobial activity was evident against all test organisms for up to 5 days and, in some cases, up to 12 days following an on-going microbial challenge. Imaging bacteria sequestered in the silver-free Dressing showed that each microbial species aggregated in the Dressing and remained viable for more than 20 hours. Growth was not observed inside of the Dressing, indicating a possible microbiostatic effect of the Alginate fibres. In comparison, organisms in the RESTORE silver Alginate Dressing were seen to lose viability at a considerably greater rate. After 16 hours of contact with the RESTORE silver Alginate Dressing, >90% of cells of all bacteria and yeast were no longer viable. In conclusion, collectively, the data highlights the rapid speed of kill and antimicrobial suitability of this RESTORE silver Alginate Dressing on wound isolates and highlights its overwhelming ability to manage a microbial wound bioburden in the management of infected wounds.
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the antimicrobial efficacy of a silver Alginate Dressing against a broad spectrum of clinically relevant wound isolates
International Wound Journal, 2011Co-Authors: Steven L Percival, W Slone, S Linton, T Okel, L Corum, J G ThomasAbstract:: Wound Dressings impregnated with silver have a role to play in aiding to reduce both the Dressing and wound microbial bioburden. It is therefore imperative that antimicrobial wound Dressings have efficacy on a broad range of clinical significant microorganisms. Accordingly, this study aimed to determine the antimicrobial efficacy of a silver Alginate Dressing against 115 wound isolates that had been isolated routinely from patients at West Virginia University Hospital. Standardised corrected zones of inhibition (CZOIs) were performed on all clinical isolates. It was found that the silver Alginate Dressing was able to inhibit the growth of all microorganisms tested. In particular, the silver Alginate Dressing inhibited the growth of Candida albicans and yeasts with CZOI of 3-11·5 mm. All meticillin-resistant Staphylococcus aureus (MRSA) strains were found to be sensitive to the silver Alginate Dressing with a CZOI range calculated at 3-7·8 mm. Sensitivity to the silver Alginate Dressing was also evident for S. aureus and vancomycin-resistant Enterococci. CZOIs of 4·25 mm were calculated for Enterococcus faecium and 9·8 mm for viridans streptococcus. The bacteria which demonstrated the highest tolerance to ionic silver included Enterobacter cloacae and Acinetobacter baumannii. Contrary to this the most responsive microorganisms to ionic silver included strains of staphylococci, viridans streptococcus and Candida albicans. No antibiotic-resistant isolates, as identified by Kirby Bauer Clinical Laboratory Standards Institute classification system, were found to be resistant to ionic silver. When a selected number of microorganisms were grown in the biofilm phenotypic state enhanced tolerance to silver was observed, compared to their non biofilm counterparts. Overall, this study has demonstrated the broad antimicrobial activity of a silver Alginate Dressing on wound isolates grown in the non biofilm and biofilm state. This finding is clinically relevant as both the non biofilm and biofilm phenotypic states of microorganisms are evident in wounds and therefore significant to delayed healing. Consequently, it is imperative that antimicrobial wound Dressings demonstrate antimicrobial activity against microorganisms in both phenotypic states.
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in vitro antimicrobial efficacy of a silver Alginate Dressing on burn wound isolates
Journal of Wound Care, 2011Co-Authors: J G Thomas, W Slone, S Linton, T Okel, L Corum, Steven L PercivalAbstract:Objective: To test the antimicrobial effectiveness of a silver Alginate Dressing on opportunistic pathogens, namely meticillin-sensitive Staphylococcus aureus (MSSA) and meticillin-resistant Staphylococcus aureus (MRSA), Klebsiella spp., Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Escherichia coli, Enterobacter sakazakii, Enterobacter cloacae, Serratia marcescens, Chryseobacterium indologenes, Proteus vulgaris and Acinetobacter baumannii. Method: In total, 40 microorganisms were isolated from patients attending three burn centres in the US and evaluated for their susceptibility to a silver Alginate wound Dressing, employing a corrected zone of inhibition assay, conducted on Mueller Hinton agar (MHA). Results: The sizes of the corrected zones of inhibition varied between and within genera. For example, all Acinetobacter baumannii strains were found to be sensitive to ionic silver at PH 7, with a mean of 2.8mm, compared with 3.5mm at PH 5.5. The silver Alginate Dressing also demonst...
J G Thomas - One of the best experts on this subject based on the ideXlab platform.
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In vitro antimicrobial efficacy of a silver Alginate Dressing on burn wound isolates.
Journal of wound care, 2020Co-Authors: J G Thomas, W Slone, S Linton, T Okel, L Corum, S L PercivalAbstract:To test the antimicrobial effectiveness of a silver Alginate Dressing on opportunistic pathogens, namely meticillin-sensitive Staphylococcus aureus (MSSA) and meticillin-resistant Staphylococcus aureus (MRSA), Klebsiella spp., Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Escherichia coli, Enterobacter sakazakii, Enterobacter cloacae, Serratia marcescens, Chryseobacterium indologenes, Proteus vulgaris and Acinetobacter baumannii. In total, 40 microorganisms were isolated from patients attending three burn centres in the US and evaluated for their susceptibility to a silver Alginate wound Dressing, employing a corrected zone of inhibition assay, conducted on Mueller Hinton agar (MHA). The sizes of the corrected zones of inhibition varied between and within genera. For example, all Acinetobacter baumannii strains were found to be sensitive to ionic silver at pH 7, with a mean of 2.8mm, compared with 3.5mm at pH 5.5. The silver Alginate Dressing also demonstrated activity on all strains of Enterobacter and Escherichia coli, with susceptibility to the silver Alginate Dressing enhanced at pH 5.5. For Enterococcus spp. the average corrected zone of inhibition at pH 7 was 3.6mm, versus 4.9mm at pH 5.5. All strains of Pseudomonas aeruginosa were found to be sensitive to the silver Alginate Dressing. The average corrected zone of inhibition was 6.9mm at pH 7, compared with 8mm at pH 5.5. For MRSA and Staphylococcus aureus, it ranged from 4.5mm to 7.5mm at pH 7. When the pH was decreased to 5.5, the corrected zone of inhibition increased. This study demonstrates the activity of a silver Alginate Dressing on a wide range of burn isolates, including antibiotic-resistant bacteria, isolated from three different burn centres in the US. It also highlights the possible importance of pH and its potential effects on antimicrobial performance and microbial susceptibility. However, more extensive testing is required to substantiate this. SLP is employed by Advanced Medical Solutions Ltd.
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the antimicrobial efficacy of a silver Alginate Dressing against a broad spectrum of clinically relevant wound isolates
International Wound Journal, 2011Co-Authors: Steven L Percival, W Slone, S Linton, T Okel, L Corum, J G ThomasAbstract:: Wound Dressings impregnated with silver have a role to play in aiding to reduce both the Dressing and wound microbial bioburden. It is therefore imperative that antimicrobial wound Dressings have efficacy on a broad range of clinical significant microorganisms. Accordingly, this study aimed to determine the antimicrobial efficacy of a silver Alginate Dressing against 115 wound isolates that had been isolated routinely from patients at West Virginia University Hospital. Standardised corrected zones of inhibition (CZOIs) were performed on all clinical isolates. It was found that the silver Alginate Dressing was able to inhibit the growth of all microorganisms tested. In particular, the silver Alginate Dressing inhibited the growth of Candida albicans and yeasts with CZOI of 3-11·5 mm. All meticillin-resistant Staphylococcus aureus (MRSA) strains were found to be sensitive to the silver Alginate Dressing with a CZOI range calculated at 3-7·8 mm. Sensitivity to the silver Alginate Dressing was also evident for S. aureus and vancomycin-resistant Enterococci. CZOIs of 4·25 mm were calculated for Enterococcus faecium and 9·8 mm for viridans streptococcus. The bacteria which demonstrated the highest tolerance to ionic silver included Enterobacter cloacae and Acinetobacter baumannii. Contrary to this the most responsive microorganisms to ionic silver included strains of staphylococci, viridans streptococcus and Candida albicans. No antibiotic-resistant isolates, as identified by Kirby Bauer Clinical Laboratory Standards Institute classification system, were found to be resistant to ionic silver. When a selected number of microorganisms were grown in the biofilm phenotypic state enhanced tolerance to silver was observed, compared to their non biofilm counterparts. Overall, this study has demonstrated the broad antimicrobial activity of a silver Alginate Dressing on wound isolates grown in the non biofilm and biofilm state. This finding is clinically relevant as both the non biofilm and biofilm phenotypic states of microorganisms are evident in wounds and therefore significant to delayed healing. Consequently, it is imperative that antimicrobial wound Dressings demonstrate antimicrobial activity against microorganisms in both phenotypic states.
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in vitro antimicrobial efficacy of a silver Alginate Dressing on burn wound isolates
Journal of Wound Care, 2011Co-Authors: J G Thomas, W Slone, S Linton, T Okel, L Corum, Steven L PercivalAbstract:Objective: To test the antimicrobial effectiveness of a silver Alginate Dressing on opportunistic pathogens, namely meticillin-sensitive Staphylococcus aureus (MSSA) and meticillin-resistant Staphylococcus aureus (MRSA), Klebsiella spp., Enterococcus faecalis, Enterococcus faecium, Pseudomonas aeruginosa, Escherichia coli, Enterobacter sakazakii, Enterobacter cloacae, Serratia marcescens, Chryseobacterium indologenes, Proteus vulgaris and Acinetobacter baumannii. Method: In total, 40 microorganisms were isolated from patients attending three burn centres in the US and evaluated for their susceptibility to a silver Alginate wound Dressing, employing a corrected zone of inhibition assay, conducted on Mueller Hinton agar (MHA). Results: The sizes of the corrected zones of inhibition varied between and within genera. For example, all Acinetobacter baumannii strains were found to be sensitive to ionic silver at PH 7, with a mean of 2.8mm, compared with 3.5mm at PH 5.5. The silver Alginate Dressing also demonst...
H H Steenfos - One of the best experts on this subject based on the ideXlab platform.
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a fibre free Alginate Dressing in the treatment of split thickness skin graft donor sites
Journal of The European Academy of Dermatology and Venereology, 1998Co-Authors: H H Steenfos, M S AgrenAbstract:Background Alginate Dressings are gaining acceptance in wound management although comparative published data with conventional treatment is inconclusive. Aims The aim of this randomised controlled study was to compare a fibre-free Alginate Dressing (Comfeel SeaSorb) with conventional treatment of standardised split-thickness skin graft donor sites in 17 patients regarding initial absorption of blood and healing. Results The Alginate Dressing absorbed 40% (P < 0.05) more blood, measured as total iron content of used Dressings, during the first 10 post-wounding minutes than fine mesh gauze, resulting in less subsequent bleeding. Light microscopic examination of punch biopsies obtained from 10 wounds on post-operative day 6 demonstrated that nine wounds treated with the Alginate Dressing compared with seven wounds treated conventionally with paraffin-impregnated gauze (Jelonet8) were completely epithelialised, a statistically non-significant difference (P = 0.46). Conclusions In conclusion, the fibre-free Alginate Dressing showed increased initial blood absorption resulting in quicker haemostasis but showed no greater beneficial effect on epithelialisation of split-thickness skin graft donor sites compared with conventional topical treatment. 0 1998 Elsevier Science B.V. All rights reserved
Yoshimi Kakimaru - One of the best experts on this subject based on the ideXlab platform.
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in vivo evaluation of a novel Alginate Dressing
Journal of Biomedical Materials Research, 1999Co-Authors: Yoshihisa Suzuki, Masao Tanihara, Yoshihiko Nishimura, Kyoko Suzuki, Yoshio Yamawaki, Hitoshi Kudo, Yoshimi Kakimaru, Yasuhiko ShimizuAbstract:Alginate Dressings are currently used in the management of epidermal and dermal wounds, and provide a moist environment that leads to rapid granulation and reepithelialization. However, a cytotoxic effect on proliferation of fibroblasts and residual material with inflammation in healing wounds have been reported recently. We have developed a new Alginate Dressing (AGA-100), which does not have an inhibitory effect on proliferation of fibroblasts. The purpose of this study was to evaluate the new Alginate Dressing with respect to wound healing in full- and partial-thickness pig wounds and with respect to biodegradation following implantation into rabbit muscle. Kaltostat and Sorbsan, both well-established commercial Dressings, were used as control. The closure rate of full-thickness wounds treated with AGA-100 was significantly higher on day 15 compared with that with Kaltostat and Sorbsan. Reepithelialization rate of partial-thickness wounds treated with Sorbsan was statistically significantly lower on day 3 than those with the other two Dressings. As to Dressing debris remained in the healing wound, a large amount of foreign debris was noted in all the full-thickness wounds treated with Kaltostat or Sorbsan, while only about one-third of wounds treated with AGA-100 showed a little Dressing debris. AGA-100 implanted into the muscle of rabbits was bioresorbed completely within 3 months. Therefore, Dressing residue in AGA-100-treated full-thickness wounds might be fully absorbed in a few months. In conclusion, it is shown that our newly developed AGA-100 possesses superior properties compared with typical Alginate Dressings. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res (Appl Biomater) 48: 522–527, 1999
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Development of Alginate gel Dressing
Journal of Artificial Organs, 1998Co-Authors: Yoshihisa Suzuki, Masao Tanihara, Yoshihiko Nishimura, Kyoko Suzuki, Yoshio Yamawaki, Yasuhiko Shimizu, Américo Kiyoshi Kitahara, Tatsuo Nakamura, Yoshimi KakimaruAbstract:Alginate-based wound Dressing materials have been widely used to promote wound healing and to reduce blood loss from wounds. However, recently a few drawbacks of well-established commercial Alginate Dressings have been reported. Therefore, we tried to develop a new Alginate Dressing to reduce the drawbacks. First, four new Dressings with different calcium content were prepared, and the cytotoxicity of these four materials, and Kaltostat and Sorbsan, was tested in vitro by culture of fibroblasts with their extracts. Second, full-thickness wounds in pigs were used for the evaluation of wound healing in vivo. Finally, a newly developed Alginate Dressing was used clinically for treatment of split-thickness skin graft donor sites. The extract medium from ALG3, ALG4, Kaltostat, and Sorbsan induced a significant inhibitory effect on proliferation of fibroblasts. As for wound closure rate, the ALG2-covered wounds had the smallest wound area on day 15. Histologically, foreign-body reaction was least in ALG2-treated wounds. In a clinical study, the main drawback of ALG2 was leakage of wound exudate due to dissolution of the Dressing material. However, the transparency of moistened ALG2 allowed easy evaluation of the wound, and after healing it was easy to remove ALG2 from the wound without injury to the reepithelialized skin because ALG2 was relatively nonadherent to the wound.
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evaluation of a novel Alginate gel Dressing cytotoxicity to fibroblasts in vitro and foreign body reaction in pig skin in vivo
Journal of Biomedical Materials Research, 1998Co-Authors: Yoshihisa Suzuki, Masao Tanihara, Yoshihiko Nishimura, Kyoko Suzuki, Yoshio Yamawaki, Yasuhiko Shimizu, Tatsuo Nakamura, Yoshimi KakimaruAbstract:Calcium Alginate Dressings have beneficial effects on wound healing by providing a moist wound environment. However, cytotoxicity and the nonbiodegradable nature of calcium Alginate Dressings induce unresolved chronic foreign-body reaction. In this study, a novel freeze-dried Alginate gel Dressing (AGA-100) low in calcium ions was evaluated for cytotoxicity to L929 cells in vitro and in full-thickness pig wounds in vivo. Cytotoxicity testing on L929 cells showed the cytocompatibility of AGA-100 extracts, while extracts from Kaltostat, a well-established Alginate Dressing, induced cytopathic effects. In an in vivo study using pigskin, AGA-100, Kaltostat, and gauze were applied on 1-in-diameter circular full-thickness wounds on the back of pigs and the time course of wound closure was evaluated. Kaltostat and gauze Dressings were used as controls. For histologic evaluation, wound tissue was harvested on day 18. AGA-100-treated wounds showed rapid wound closure compared to control wounds on day 15. Foreign-body reaction was marked in Kaltostat- and gauze-treated wounds, and differed significantly from AGA-100-treated wounds. Based on these data, AGA-100 could reduce the cytotoxicity to fibroblasts and foreign-body reaction that have been observed with currently available calcium Alginate Dressings; it was also found to be useful as an Alginate Dressing. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 317–322, 1998.
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Evaluation of a novel Alginate gel Dressing: Cytotoxicity to fibroblasts in vitro and foreign‐body reaction in pig skin in vivo
Journal of Biomedical Materials Research, 1998Co-Authors: Yoshihisa Suzuki, Masao Tanihara, Yoshihiko Nishimura, Kyoko Suzuki, Yoshio Yamawaki, Yasuhiko Shimizu, Tatsuo Nakamura, Yoshimi KakimaruAbstract:Calcium Alginate Dressings have beneficial effects on wound healing by providing a moist wound environment. However, cytotoxicity and the nonbiodegradable nature of calcium Alginate Dressings induce unresolved chronic foreign-body reaction. In this study, a novel freeze-dried Alginate gel Dressing (AGA-100) low in calcium ions was evaluated for cytotoxicity to L929 cells in vitro and in full-thickness pig wounds in vivo. Cytotoxicity testing on L929 cells showed the cytocompatibility of AGA-100 extracts, while extracts from Kaltostat, a well-established Alginate Dressing, induced cytopathic effects. In an in vivo study using pigskin, AGA-100, Kaltostat, and gauze were applied on 1-in-diameter circular full-thickness wounds on the back of pigs and the time course of wound closure was evaluated. Kaltostat and gauze Dressings were used as controls. For histologic evaluation, wound tissue was harvested on day 18. AGA-100-treated wounds showed rapid wound closure compared to control wounds on day 15. Foreign-body reaction was marked in Kaltostat- and gauze-treated wounds, and differed significantly from AGA-100-treated wounds. Based on these data, AGA-100 could reduce the cytotoxicity to fibroblasts and foreign-body reaction that have been observed with currently available calcium Alginate Dressings; it was also found to be useful as an Alginate Dressing. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 317–322, 1998.
