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Alberto A. Guglielmone - One of the best experts on this subject based on the ideXlab platform.
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Amblyomma ticks found on cattle in the northwest of Argentina
'EDP Sciences', 2017Co-Authors: Alberto A. Guglielmone, A. HadaniAbstract:The phytogeographical and seasonal distributions of three species of Amblyomma ticks found on cattle are presented. The material studied was collected during a four-year survey in the Northwest of Argentina. Amblyomma neumanni shows predilection for the Chaqueno Serrano district although it was also found in ecotones (phytogeographical transition zones) of the adjacent Western Chaqueno district and the province of Las Yungas. Amblyomma Cajennense was found in the Western Chaqueno and Chaqueno Serrano districts as well as in the province of Las Yungas. Amblyomma parvum was found in all the Western Chaqueno district and in the northern driest part of the Chaqueno Serrano district. Amblyomma neumanni seems to be inactive in summer while A. parvum adults predominate in summer and disappear in winter. The scarcity of the preimaginal stages of A. parvum on cattle suggests that these stages prefer non-bovine hosts
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divergent environmental preferences and areas of sympatry of tick species in the Amblyomma Cajennense complex ixodidae
International Journal for Parasitology, 2014Co-Authors: Agustin Estradapena, Alberto A. Guglielmone, Atilio J. Mangold, Evelina Luisa Tarragona, Umberto Vesco, Daniele De Meneghi, Mariano Mastropaolo, Santiago NavaAbstract:Four species of Neotropical ticks, Amblyomma mixtum, Amblyomma Cajennense, Amblyomma tonelliae and Amblyomma sculptum (formerly included in the catch-all name A. Cajennense), have an allopatric distribution in much of their range, with areas of parapatry for at least two of them. We inferred the abiotic niches of these organisms using coefficients of a harmonic regression of the temperature and the Normalized Difference Vegetation Index (NDVI, reflecting plant stress) from remotely sensed data from MODIS satellites with 0.05° spatial resolution. Combinations of coefficients describing the phenology of these two variables pointed to divergent niche preferences, compatible with previous events of vicariance among the species. Amblyomma Cajennense has been recorded in areas with small variations in temperature and NDVI. The remaining species were recorded in areas with large variations. The maximum environmental niche overlap was ∼73.6% between A. mixtum and A. Cajennense and 73.5% between A. tonelliae and A. sculptum. Projecting these inferences on the geographical space revealed probable areas of sympatry or parapatry between A. mixtum and A. Cajennense or between A. tonelliae and A. sculptum, the latter of which was confirmed with field collections. The A. sculptum distribution overlaps with that of A. tonelliae in northern Argentina and Paraguay; parapatry occurs at one extreme of the conditions occupied by both species. Compared with areas of allopatry, sites with both species had consistently lower temperatures, except for 10-12weeks during the summer, and higher NDVI values throughout the year. We hypothesise that the overlap between A. tonelliae and A. sculptum resulted from secondary contact between populations, with A. sculptum adapting to sites with high water availability to balance high summer temperatures. Additional surveys of the areas of spatial overlap among these species are necessary to elucidate the forces driving their evolution and their adaptation to the environment.
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reassessment of the taxonomic status of Amblyomma Cajennense fabricius 1787 with the description of three new species Amblyomma tonelliae n sp Amblyomma interandinum n sp and Amblyomma patinoi n sp and reinstatement of Amblyomma mixtum koch 1844 and
Ticks and Tick-borne Diseases, 2014Co-Authors: Santiago Nava, Lorenza Beati, Atilio J. Mangold, Abraham G Caceres, Alberto A. GuglielmoneAbstract:A reassessment of the taxonomic status of Amblyomma Cajennense based on the morphological analyses of ticks from the whole distribution area of the species resulted in the redescription of A. Cajennense, the validation of 2 species which had been reduced to synonymy in the past, Amblyomma mixtum and Amblyomma sculptum, and the description and definition of 3 new species, Amblyomma tonelliae n. sp., Amblyomma interandinum n. sp., and Amblyomma patinoi n. sp. This study provides descriptions and redescriptions, scanning electron microscopic and stereomicroscopic images, updated synonymies, information on geographical distributions, and host associations for each of the 6 species. Amblyomma Cajennense s.s. is found in the Amazonian region of South America, A. interandinum is reported from the northern part of the Inter-Andean valley of Peru, A. mixtum is present from Texas (U.S.A.) to western Ecuador, A. patinoi occurs in the Eastern Cordillera of Colombia, A. tonelliae is associated with the dry areas of the Chaco region which spans from central-northern Argentina to Bolivia and Paraguay, whereas A. sculptum is distributed from the humid areas of northern Argentina, to the contiguous regions of Bolivia and Paraguay and the coastal and central-western states of Brazil.
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Amblyomma Cajennense fabricius 1787 acari ixodidae the cayenne tick phylogeography and evidence for allopatric speciation
BMC Evolutionary Biology, 2013Co-Authors: Lorenza Beati, Darci Moraes Barrosbattesti, Santiago Nava, Alberto A. Guglielmone, Abraham G Caceres, Erica Burkman, Marcelo B Labruna, Carmen Guzmancornejo, Renato LeonAbstract:Background Amblyomma Cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. Cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics.
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Amblyomma Cajennense fabricius 1787 acari ixodidae the cayenne tick phylogeography and evidence for allopatric speciation
BMC Evolutionary Biology, 2013Co-Authors: Lorenza Beati, Darci Moraes Barrosbattesti, Santiago Nava, Alberto A. Guglielmone, Abraham G Caceres, Erica Burkman, Marcelo B Labruna, Carmen Guzmancornejo, Renato LeonAbstract:Amblyomma Cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. Cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics. Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. Cajennense. The phylogenetic analyses consistently found A. Cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene. Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. Cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. Cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics.
Marcelo B Labruna - One of the best experts on this subject based on the ideXlab platform.
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Rickettsia Amblyommatis infecting ticks and exposure of domestic dogs to Rickettsia spp. in an Amazon-Cerrado transition region of northeastern Brazil
2017Co-Authors: Francisco B Costa, Thiago F Martins, Jonas Moraes-filho, Herbert S. Soares, Andréa P. Da Costa, Diego G. Ramirez, Ricardo A. Dias, Marcelo B LabrunaAbstract:This study was performed in Maranhão state, a transition area two Brazilian biomes, Amazon and Cerrado. During 2011–2013, 1,560 domestic dogs were sampled for collection of serum blood samples and ticks in eight counties (3 within the Amazon and 5 within the Cerrado). A total of 959 ticks were collected on 150 dogs (9.6%). Rhipicephalus sanguineus sensu lato (s.l.) was the most abundant tick (68% of all collected specimens), followed by Amblyomma Cajennense sensu lato (s.l.) (12.9%), Amblyomma parvum (9.2%), and Amblyomma ovale (5.2%). Other less abundant species (
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Amblyomma Cajennense fabricius 1787 acari ixodidae the cayenne tick phylogeography and evidence for allopatric speciation
BMC Evolutionary Biology, 2013Co-Authors: Lorenza Beati, Darci Moraes Barrosbattesti, Santiago Nava, Alberto A. Guglielmone, Abraham G Caceres, Erica Burkman, Marcelo B Labruna, Carmen Guzmancornejo, Renato LeonAbstract:Background Amblyomma Cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. Cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics.
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Amblyomma Cajennense fabricius 1787 acari ixodidae the cayenne tick phylogeography and evidence for allopatric speciation
BMC Evolutionary Biology, 2013Co-Authors: Lorenza Beati, Darci Moraes Barrosbattesti, Santiago Nava, Alberto A. Guglielmone, Abraham G Caceres, Erica Burkman, Marcelo B Labruna, Carmen Guzmancornejo, Renato LeonAbstract:Amblyomma Cajennense F. is one of the best known and studied ticks in the New World because of its very wide distribution, its economical importance as pest of domestic ungulates, and its association with a variety of animal and human pathogens. Recent observations, however, have challenged the taxonomic status of this tick and indicated that intraspecific cryptic speciation might be occurring. In the present study, we investigate the evolutionary and demographic history of this tick and examine its genetic structure based on the analyses of three mitochondrial (12SrDNA, d-loop, and COII) and one nuclear (ITS2) genes. Because A. Cajennense is characterized by a typical trans-Amazonian distribution, lineage divergence dating is also performed to establish whether genetic diversity can be linked to dated vicariant events which shaped the topology of the Neotropics. Total evidence analyses of the concatenated mtDNA and nuclear + mtDNA datasets resulted in well-resolved and fully congruent reconstructions of the relationships within A. Cajennense. The phylogenetic analyses consistently found A. Cajennense to be monophyletic and to be separated into six genetic units defined by mutually exclusive haplotype compositions and habitat associations. Also, genetic divergence values showed that these lineages are as distinct from each other as recognized separate species of the same genus. The six clades are deeply split and node dating indicates that they started diverging in the middle-late Miocene. Behavioral differences and the results of laboratory cross-breeding experiments had already indicated that A. Cajennense might be a complex of distinct taxonomic units. The combined and congruent mitochondrial and nuclear genetic evidence from this study reveals that A. Cajennense is an assembly of six distinct species which have evolved separately from each other since at least 13.2 million years ago (Mya) in the earliest and 3.3 Mya in the latest lineages. The temporal and spatial diversification modes of the six lineages overlap the phylogeographical history of other organisms with similar extant trans-Amazonian distributions and are consistent with the present prevailing hypothesis that Neotropical diversity often finds its origins in the Miocene, after the Andean uplift changed the topology and consequently the climate and ecology of the Neotropics.
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Cross-mating experiments with geographically different populations of Amblyomma Cajennense (Acari: Ixodidae)
Experimental and Applied Acarology, 2011Co-Authors: Marcelo B Labruna, Thiago F Martins, Joao F Soares, Herbert S. Soares, Ricardo R. CabreraAbstract:The present study evaluated the reproductive compatibility of the crosses between adult ticks of the following three geographically different populations of Amblyomma Cajennense : State of São Paulo (SP), southeastern Brazil; State of Rondônia (RO), northern Brazil; and Colombia (CO). In addition, crosses between A. Cajennense ticks from Argentina (AR) and SP ticks were also performed. The Argentinean population (AR) was compatible with SP because their crosses resulted in high % egg hatching (mean values ranging from 71.5 to 93.5%), similarly to all homologous (intrapopulational) crosses. In contrast, the tick populations SP, RO, and CO were shown to be incompatible with each other, since their heterologous (interpopulational) crosses always resulted in very low % egg hatching (range: 0–5%). The F_1 larval offspring derived from some of these females that yielded 5% egg hatching were reared until the F_1 adult stage. In all cases, only adult females molted from engorged nymphs. These F_1 females were likely to be a product of thelytokous parthenogenesis of the SP, RO, and CO females that were used in the heterologous crosses. Reproductive incompatibility is not expected to occur between different populations of a single species. Thus, our results suggest that the taxon A. Cajennense might be represented by a complex of different species, whereas SP and AR ticks might represent a single species. Further populational genetic studies, coupled with extensive morphological analyses, are needed to clarify and determine a possible complex of valid species that might have been classified under the taxon A. Cajennense.
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experimental infection of capybaras hydrochoerus hydrochaeris by rickettsia rickettsii and evaluation of the transmission of the infection to ticks Amblyomma Cajennense
Veterinary Parasitology, 2009Co-Authors: Celso Eduardo De Souza, Mauricio Claudio Horta, Jonas Moraesfilho, Maria Ogrzewalska, Franscisco C Uchoa, Savina Silvana Aparecida Lacerra De Souza, Renata C M Borba, Marcelo B LabrunaAbstract:The present study evaluated the infection of capybaras (Hydrochoerus hydrochaeris) by Rickettsia rickettsii and their role as amplifier hosts for horizontal transmission of R. rickettsii to Amblyomma Cajennense ticks. Two groups of two capybaras each were evaluated: on day 0, group 1 (G1) was infested by R. rickettsii-infected ticks, and group 2 (G2) was inoculated intraperitoneally with R. rickettsii. Two additional groups were control groups, not exposed to R. rickettsii, being CG1 group the control of G1, and CG2 group the control of G2. Capybara rectal temperature was measured daily. Blood samples were collected every 3 days during 30 days, and used to (i) inoculate guinea pigs intraperitoneally; (ii) DNA extraction followed by real-time PCR targeting the rickettsial gene gltA; (iii) hematology; (iv) detection of R. rickettsii-reactive antibodies by indirect immunofluorescence assay (IFA). Blood was also collected from G1 capybaras every ≈10–30 days till the 146th day, to be tested by serology. Capybaras were infested by uninfected A. Cajennense nymphs from the 3rd to the 18th day. Engorged nymphs were collected, allowed to molt to adults in an incubator. Thereafter, the subsequent flat ticks were tested by PCR. All G1 and G2 capybaras became infected by R. rickettsii, as demonstrated by guinea pig inoculation and seroconversion, but they showed no fever. Rickettsemia was continually detected from the 6th (G2 capybaras) or 9th (G1 capybaras) to the 18th day post inoculation or infestation with R. rickettsii-infected ticks. A total of 20–25% and 30–35% of the flat ticks previously fed on G1 and G2 capybaras, respectively, became infected by R. rickettsii. The study demonstrated that R. rickettsii was capable to infect capybaras without causing clinical illness, inducing rickettsemia capable to cause infection in guinea pigs and ticks. Our results indicate that capybaras act as amplifier host of R. rickettsii for A. Cajennense ticks in Brazil.
Thiago F Martins - One of the best experts on this subject based on the ideXlab platform.
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Rickettsia Amblyommatis infecting ticks and exposure of domestic dogs to Rickettsia spp. in an Amazon-Cerrado transition region of northeastern Brazil
2017Co-Authors: Francisco B Costa, Thiago F Martins, Jonas Moraes-filho, Herbert S. Soares, Andréa P. Da Costa, Diego G. Ramirez, Ricardo A. Dias, Marcelo B LabrunaAbstract:This study was performed in Maranhão state, a transition area two Brazilian biomes, Amazon and Cerrado. During 2011–2013, 1,560 domestic dogs were sampled for collection of serum blood samples and ticks in eight counties (3 within the Amazon and 5 within the Cerrado). A total of 959 ticks were collected on 150 dogs (9.6%). Rhipicephalus sanguineus sensu lato (s.l.) was the most abundant tick (68% of all collected specimens), followed by Amblyomma Cajennense sensu lato (s.l.) (12.9%), Amblyomma parvum (9.2%), and Amblyomma ovale (5.2%). Other less abundant species (
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geographical distribution of Amblyomma Cajennense sensu lato ticks parasitiformes ixodidae in brazil with description of the nymph of a Cajennense sensu stricto
Parasites & Vectors, 2016Co-Authors: Thiago F Martins, Richard C. Pacheco, Francisco B Costa, Amalia R M Barbieri, Flavio A Terassini, Luis Marcelo Aranha Camargo, Cassio Peterka, Ricardo Augusto Dias, Pablo Henrique Nunes, Arlei MarciliAbstract:Until recently, Amblyomma Cajennense (Fabricius, 1787) was considered to represent a single tick species in the New World. Recent studies have split this taxon into six species. While the A. Cajennense species complex or A. Cajennense (sensu lato) (s.l.) is currently represented by two species in Brazil, A. Cajennense (sensu stricto) (s.s.) and Amblyomma sculptum Berlese, 1888, their geographical distribution is poorly known. The distribution of the A. Cajennense (s.l.) in Brazil was determined by morphological examination of all lots of A. Cajennense (s.l.) in two large tick collections of Brazil, and by collecting new material during three field expeditions in the possible transition areas between the distribution ranges of A. Cajennense (s.s.) and A. sculptum. Phylogenetic analysis inferred from the ITS2 rRNA gene was used to validate morphological results. Morphological description of the nymphal stage of A. Cajennense (s.s.) is provided based on laboratory-reared specimens. From the tick collections, a total 12,512 adult ticks were examined and identified as 312 A. Cajennense (s.s.), 6,252 A. sculptum and 5,948 A. Cajennense (s.l.). A total of 1,746 ticks from 77 localities were collected during field expeditions, and were identified as 249 A. Cajennense (s.s.), 443 A. sculptum, and 1,054 A. Cajennense (s.l.) [these A. Cajennense (s.l.) ticks were considered to be males of either A. Cajennense (s.s.) or A. sculptum]. At least 23 localities contained the presence of both A. Cajennense (s.s.) and A. sculptum in sympatry. DNA sequences of the ITS2 gene of 50 ticks from 30 localities confirmed the results of the morphological analyses. The nymph of A. Cajennense (s.s.) is morphologically very similar to A. sculptum. Our results confirmed that A. Cajennense (s.l.) is currently represented in Brazil by only two species, A. Cajennense (s.s.) and A. sculptum. While these species have distinct distribution areas in the country, they are found in sympatry in some transition areas. The current distribution of A. Cajennense (s.l.) has important implications to public health, since in Brazil A. sculptum is the most important vector of the bacterium Rickettsia rickettsii, the etiological agent of Brazilian spotted fever.
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geographical distribution of Amblyomma Cajennense sensu lato ticks parasitiformes ixodidae in brazil with description of the nymph of a Cajennense sensu stricto
Parasites & Vectors, 2016Co-Authors: Thiago F Martins, Richard C. Pacheco, Francisco B Costa, Amalia R M Barbieri, Flavio A Terassini, Luis Marcelo Aranha Camargo, Cassio Peterka, Ricardo Augusto Dias, Pablo Henrique Nunes, Arlei MarciliAbstract:Background Until recently, Amblyomma Cajennense (Fabricius, 1787) was considered to represent a single tick species in the New World. Recent studies have split this taxon into six species. While the A. Cajennense species complex or A. Cajennense (sensu lato) (s.l.) is currently represented by two species in Brazil, A. Cajennense (sensu stricto) (s.s.) and Amblyomma sculptum Berlese, 1888, their geographical distribution is poorly known.
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Ticks (Acari: Ixodidae) associated with small terrestrial mammals in the state of Minas Gerais, southeastern Brazil
Experimental and Applied Acarology, 2012Co-Authors: Danilo Goncalves Saraiva, Thiago F Martins, Valeria C Onofrio, Darci Moraes Barros-battesti, Gislene F S R Fournier, Karla P G Leal, Flavia N Vieira, Edeltrudes M V C Câmara, Claudia Guimaraes Costa, Alberto A. GuglielmoneAbstract:From June 2005 to November 2010, 43 small mammals encompassing 6 species of Didelphimorphia, 8 species of Rodentia, and 1 species of Lagomorpha were found parasitized by ticks in the state of Minas Gerais, southeastern Brazil. Nine tick species, in total 186 specimens, were identified as follows: Amblyomma Cajennense (larvae and nymphs) on opossums and rodents; Amblyomma ovale (nymphs) on rodents; Amblyomma parvum (nymphs) on rodents; Amblyomma coelebs (nymphs) on opossums; Amblyomma dubitatum (nymph) on opossums; Ixodes amarali (females, nymphs, and larvae) on opossums and rodents; Ixodes loricatus (male, females, nymph) on opossums; Ixodes schulzei (female) on rodents; and Haemaphysalis leporispalustris (female) on rabbits. Most of the tick-host associations found in the present study have never been recorded in the literature; those include three new host records for I. amarali , four for A. Cajennense , one for A. dubitatum , two for A. ovale , and one for A. coelebs . In addition, we provide the first record of A. coelebs in the state of Minas Gerais.
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ticks acari ixodidae associated with small terrestrial mammals in the state of minas gerais southeastern brazil
Experimental and Applied Acarology, 2012Co-Authors: Danilo Goncalves Saraiva, Thiago F Martins, Valeria C Onofrio, Darci Moraes Barrosbattesti, Gislene Fatima Da Silva Rocha Fournier, Karla P G Leal, Flavia N Vieira, Edeltrudes M V C Câmara, Claudia Guimaraes Costa, Alberto A. GuglielmoneAbstract:From June 2005 to November 2010, 43 small mammals encompassing 6 species of Didelphimorphia, 8 species of Rodentia, and 1 species of Lagomorpha were found parasitized by ticks in the state of Minas Gerais, southeastern Brazil. Nine tick species, in total 186 specimens, were identified as follows: Amblyomma Cajennense (larvae and nymphs) on opossums and rodents; Amblyomma ovale (nymphs) on rodents; Ambly- omma parvum (nymphs) on rodents; Amblyomma coelebs (nymphs) on opossums; Amblyomma dubitatum (nymph) on opossums; Ixodes amarali (females, nymphs, and larvae) on opossums and rodents; Ixodes loricatus (male, females, nymph) on opossums; Ixodes schulzei (female) on rodents; and Haemaphysalis leporispalustris (female) on rabbits. Most of the tick-host associations found in the present study have never been
Arlei Marcili - One of the best experts on this subject based on the ideXlab platform.
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geographical distribution of Amblyomma Cajennense sensu lato ticks parasitiformes ixodidae in brazil with description of the nymph of a Cajennense sensu stricto
Parasites & Vectors, 2016Co-Authors: Thiago F Martins, Richard C. Pacheco, Francisco B Costa, Amalia R M Barbieri, Flavio A Terassini, Luis Marcelo Aranha Camargo, Cassio Peterka, Ricardo Augusto Dias, Pablo Henrique Nunes, Arlei MarciliAbstract:Background Until recently, Amblyomma Cajennense (Fabricius, 1787) was considered to represent a single tick species in the New World. Recent studies have split this taxon into six species. While the A. Cajennense species complex or A. Cajennense (sensu lato) (s.l.) is currently represented by two species in Brazil, A. Cajennense (sensu stricto) (s.s.) and Amblyomma sculptum Berlese, 1888, their geographical distribution is poorly known.
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geographical distribution of Amblyomma Cajennense sensu lato ticks parasitiformes ixodidae in brazil with description of the nymph of a Cajennense sensu stricto
Parasites & Vectors, 2016Co-Authors: Thiago F Martins, Richard C. Pacheco, Francisco B Costa, Amalia R M Barbieri, Flavio A Terassini, Luis Marcelo Aranha Camargo, Cassio Peterka, Ricardo Augusto Dias, Pablo Henrique Nunes, Arlei MarciliAbstract:Until recently, Amblyomma Cajennense (Fabricius, 1787) was considered to represent a single tick species in the New World. Recent studies have split this taxon into six species. While the A. Cajennense species complex or A. Cajennense (sensu lato) (s.l.) is currently represented by two species in Brazil, A. Cajennense (sensu stricto) (s.s.) and Amblyomma sculptum Berlese, 1888, their geographical distribution is poorly known. The distribution of the A. Cajennense (s.l.) in Brazil was determined by morphological examination of all lots of A. Cajennense (s.l.) in two large tick collections of Brazil, and by collecting new material during three field expeditions in the possible transition areas between the distribution ranges of A. Cajennense (s.s.) and A. sculptum. Phylogenetic analysis inferred from the ITS2 rRNA gene was used to validate morphological results. Morphological description of the nymphal stage of A. Cajennense (s.s.) is provided based on laboratory-reared specimens. From the tick collections, a total 12,512 adult ticks were examined and identified as 312 A. Cajennense (s.s.), 6,252 A. sculptum and 5,948 A. Cajennense (s.l.). A total of 1,746 ticks from 77 localities were collected during field expeditions, and were identified as 249 A. Cajennense (s.s.), 443 A. sculptum, and 1,054 A. Cajennense (s.l.) [these A. Cajennense (s.l.) ticks were considered to be males of either A. Cajennense (s.s.) or A. sculptum]. At least 23 localities contained the presence of both A. Cajennense (s.s.) and A. sculptum in sympatry. DNA sequences of the ITS2 gene of 50 ticks from 30 localities confirmed the results of the morphological analyses. The nymph of A. Cajennense (s.s.) is morphologically very similar to A. sculptum. Our results confirmed that A. Cajennense (s.l.) is currently represented in Brazil by only two species, A. Cajennense (s.s.) and A. sculptum. While these species have distinct distribution areas in the country, they are found in sympatry in some transition areas. The current distribution of A. Cajennense (s.l.) has important implications to public health, since in Brazil A. sculptum is the most important vector of the bacterium Rickettsia rickettsii, the etiological agent of Brazilian spotted fever.
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Additional file 1: Table S1. of Geographical distribution of Amblyomma Cajennense (sensu lato) ticks (Parasitiformes: Ixodidae) in Brazil, with description of the nymph of A. Cajennense (sensu stricto)
2016Co-Authors: Thiago Martins, Flavio A Terassini, Cassio Peterka, Amália Barbieri, Francisco Costa, Luís Camargo, Richard De C. Pacheco, Ricardo Dias, Pablo Nunes, Arlei MarciliAbstract:Records of Amblyomma Cajennense sensu stricto (s.s.), Amblyomma sculptum, and Amblyomma Cajennense sensu lato (s.l.) that were examined in the tick collections “Acari Colletion of the Instituto Butantan” (IBSP), São Paulo, Brazil, and “Coleção Nacional de Carrapatos” (CNC) of the University of São Paulo, São Paulo, Brazil. (PDF 1091 kb
Celso Eduardo De Souza - One of the best experts on this subject based on the ideXlab platform.
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Rickettsiae detection in Amblyomma ticks (Acari: Ixodidae) collected in the urban area of Campinas city, SP
'FapUNIFESP (SciELO)', 2015Co-Authors: Estrada, Dora Amparo, Celso Eduardo De Souza, Schumaker, Teresinha Tizu Sato, Rodrigues Neto, Elias José, Linhares, Arício XavierAbstract:The city of Campinas is located in an endemic area for brazilian spotted fever in São Paulo State, where several cases have recently occurred. Capybaras have been associated with the cycle of this disease, for they present positive serology and serve as host for ticks of the genus Amblyomma, the main vectors of brazilian spotted fever. Ticks were colleted both from Capybaras and from the vegetation in the city park Lago do Café, located in the urban area of Campinas city, SP, a site associated with suspected human cases of brazilian spotted fever. The ticks collected were examinaded for the presence of rickettsiae using polymerase chain reaction and the haemolymph test. Through analysis of the gene gltA nucleotide sequence, adults of Amblyomma Cajennense and Amblyomma cooperi were found to be infected with the non pathogenic Rickettsia bellii. However, no rickettsiae of the spotted fever group were detected. These results indicate that the role of capybaras as reservoirs of rickettsiae of the Spotted Fever group is still uncertain and further studies are required.O Município de Campinas situa-se em região endêmica para febre maculosa brasileira do Estado de São Paulo, onde vários casos desta doença vem ocorrendo. Capivaras têm sido associadas ao ciclo dessa riquetsiose por apresentarem sorologia positiva e serem hospedeiras de carrapatos Amblyomma spp principais vetores da doença. Carrapatos foram coletados no parque urbano do Lago do Café, Campinas, SP, local associado a casos humanos suspeitos de febre maculosa brasileira, sobre a vegetação e das capivaras ali presentes, e pesquisados quanto à presença de riquétsias pela reação em cadeia da polimerase e pelo teste de hemolinfa. Adultos de Amblyomma Cajennense e Amblyomma cooperi albergavam Rickettsia bellii, não patogênica, identificada pela análise das seqüências de nucleotídeos do gene gltA, porém, não foram constatadas riquétsias do Grupo da Febre Maculosa. Estes resultados associados à ausência de um isolado de riquétsias do Grupo da Febre Maculosa de capivaras indicam que seu papel, enquanto reservatório, necessita de maior investigação.687
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Seasonal dynamics of ticks (Acari:Ixodidae) in an endemic area for spotted fever in the Campinas region, state of São Paulo, Brazil
Universidade Federal de Santa Maria, 2015Co-Authors: Savina Silvana Aparecida Lacerra De Souza, Celso Eduardo De Souza, Rodrigues Neto, Elias José, Angelo Pires Do PradoAbstract:Spotted fever is recognized worldwide as a reemerging public health problem. In Campinas region, state of São Paulo, the transmission area has expanded and the number of positive cases has increased in the lastest years. In these region most cases are usually related to the increased number of capybaras, which are one of the main primary hosts for the tick Amblyomma Cajennense. The main objective of this study was to determine the seasonal dynamic of larvaes, nymphs and adults of Amblyomma species in a gallery forest. From November 2000 to October 2002 free-living ticks were collected using carbon dioxid traps. Larvae of Amblyomma spp were abundant during almost all months. Nymphs of Amblyomma spp occurred during the whole year and were more abundants from July to December. Adults of A.Cajennense were more abundants during the spring-summer months. Adults of A. dubitatum showed higher populational peaks from August to February.A febre maculosa é mundialmente reconhecida como um problema reemergente de saúde pública. Na região de Campinas-SP, observam-se uma ampliação da área de transmissão do agente da doença e a ocorrência de um maior número de casos confirmados nos últimos anos. Nesta região, a maioria dos casos desta doença está quase sempre relacionada com o aumento populacional de capivaras, que são um dos principais hospedeiros primários do estádio adulto do carrapato Amblyomma Cajennense. O principal objetivo deste estudo foi determinar o comportamento populacional de larvas, ninfas e adultos de Amblyomma spp no habitat de mata ciliar de uma área endêmica. De novembro de 2000 a outubro de 2002, carrapatos de vida livre foram coletados com armadilhas de CO2. Picos populacionais de larvas do gênero Amblyomma se estenderam por praticamente todos os meses do ano. Ninfas do gênero Amblyomma ocorreram o ano todo na mata ciliar, sendo mais abundantes de julho a dezembro. Adultos de A. Cajennense foram mais abundantes na estação de primavera e verão. Adultos de A. dubitatum (=Amblyomma cooperi) apresentaram um padrão sazonal diferenciado, com os maiores picos populacionais ocorrendo de agosto a fevereiro
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experimental infection of capybaras hydrochoerus hydrochaeris by rickettsia rickettsii and evaluation of the transmission of the infection to ticks Amblyomma Cajennense
Veterinary Parasitology, 2009Co-Authors: Celso Eduardo De Souza, Mauricio Claudio Horta, Jonas Moraesfilho, Maria Ogrzewalska, Franscisco C Uchoa, Savina Silvana Aparecida Lacerra De Souza, Renata C M Borba, Marcelo B LabrunaAbstract:The present study evaluated the infection of capybaras (Hydrochoerus hydrochaeris) by Rickettsia rickettsii and their role as amplifier hosts for horizontal transmission of R. rickettsii to Amblyomma Cajennense ticks. Two groups of two capybaras each were evaluated: on day 0, group 1 (G1) was infested by R. rickettsii-infected ticks, and group 2 (G2) was inoculated intraperitoneally with R. rickettsii. Two additional groups were control groups, not exposed to R. rickettsii, being CG1 group the control of G1, and CG2 group the control of G2. Capybara rectal temperature was measured daily. Blood samples were collected every 3 days during 30 days, and used to (i) inoculate guinea pigs intraperitoneally; (ii) DNA extraction followed by real-time PCR targeting the rickettsial gene gltA; (iii) hematology; (iv) detection of R. rickettsii-reactive antibodies by indirect immunofluorescence assay (IFA). Blood was also collected from G1 capybaras every ≈10–30 days till the 146th day, to be tested by serology. Capybaras were infested by uninfected A. Cajennense nymphs from the 3rd to the 18th day. Engorged nymphs were collected, allowed to molt to adults in an incubator. Thereafter, the subsequent flat ticks were tested by PCR. All G1 and G2 capybaras became infected by R. rickettsii, as demonstrated by guinea pig inoculation and seroconversion, but they showed no fever. Rickettsemia was continually detected from the 6th (G2 capybaras) or 9th (G1 capybaras) to the 18th day post inoculation or infestation with R. rickettsii-infected ticks. A total of 20–25% and 30–35% of the flat ticks previously fed on G1 and G2 capybaras, respectively, became infected by R. rickettsii. The study demonstrated that R. rickettsii was capable to infect capybaras without causing clinical illness, inducing rickettsemia capable to cause infection in guinea pigs and ticks. Our results indicate that capybaras act as amplifier host of R. rickettsii for A. Cajennense ticks in Brazil.
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experimental infection of capybaras hydrochoerus hydrochaeris by rickettsia rickettsii and evaluation of the transmission of the infection to ticks Amblyomma Cajennense
Veterinary Parasitology, 2009Co-Authors: Celso Eduardo De Souza, Mauricio Claudio Horta, Jonas Moraesfilho, Maria Ogrzewalska, Franscisco C Uchoa, Savina Silvana Aparecida Lacerra De Souza, Renata C M Borba, Marcelo B LabrunaAbstract:The present study evaluated the infection of capybaras (Hydrochoerus hydrochaeris) by Rickettsia rickettsii and their role as amplifier hosts for horizontal transmission of R. rickettsii to Amblyomma Cajennense ticks. Two groups of two capybaras each were evaluated: on day 0, group 1 (G1) was infested by R. rickettsii-infected ticks, and group 2 (G2) was inoculated intraperitoneally with R. rickettsii. Two additional groups were control groups, not exposed to R. rickettsii, being CG1 group the control of G1, and CG2 group the control of G2. Capybara rectal temperature was measured daily. Blood samples were collected every 3 days during 30 days, and used to (i) inoculate guinea pigs intraperitoneally; (ii) DNA extraction followed by real-time PCR targeting the rickettsial gene gltA; (iii) hematology; (iv) detection of R. rickettsii-reactive antibodies by indirect immunofluorescence assay (IFA). Blood was also collected from G1 capybaras every approximately 10-30 days till the 146th day, to be tested by serology. Capybaras were infested by uninfected A. Cajennense nymphs from the 3rd to the 18th day. Engorged nymphs were collected, allowed to molt to adults in an incubator. Thereafter, the subsequent flat ticks were tested by PCR. All G1 and G2 capybaras became infected by R. rickettsii, as demonstrated by guinea pig inoculation and seroconversion, but they showed no fever. Rickettsemia was continually detected from the 6th (G2 capybaras) or 9th (G1 capybaras) to the 18th day post inoculation or infestation with R. rickettsii-infected ticks. A total of 20-25% and 30-35% of the flat ticks previously fed on G1 and G2 capybaras, respectively, became infected by R. rickettsii. The study demonstrated that R. rickettsii was capable to infect capybaras without causing clinical illness, inducing rickettsemia capable to cause infection in guinea pigs and ticks. Our results indicate that capybaras act as amplifier host of R. rickettsii for A. Cajennense ticks in Brazil.