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Pierre-edouard Fournier - One of the best experts on this subject based on the ideXlab platform.
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Anaerococcus urinimassiliensis sp nov a new bacterium isolated from human urine
Scientific Reports, 2021Co-Authors: Aurelie Morand, E K Yimagou, Issa Isaac Ngom, Mamadou Lamine Tall, Anthony Levasseur, Jean Christophe Lagier, Didier Raoult, F Cornu, Michel Tsimaratos, Pierre-edouard FournierAbstract:To date there are thirteen species validly assigned to the genus Anaerococcus. Most of the species in this genus are anaerobic and of human origin. Anaerococcus urinimassiliensis sp. nov., strain Marseille-P2143T is member of family Peptoniphilaceae, which was isolated from the urine of a 17-year-old boy affected by autoimmune hepatitis and membranoproliferative glomerulonephritis using the culturomic approach. In the current study, a taxono-genomics method was employed to describe this new species. The strain Marseille-P2143T was gram positive cocci with translucent colonies on blood agar. Its genome was 2,189,509 bp long with a 33.5 mol% G + C content and exhibited 98.48% 16S rRNA similarity with Anaerococcus provencensis strain 9,402,080. When Anaerococcus urinomassiliensis strain Marseill-P2143T is compared with closely related species, the values ranged from 71.23% with A. hydrogenalis strain DSM 7454T (NZ_ABXA01000052.1) to 90.64% with A. provencensis strain 9402080T (NZ_HG003688.1). This strain has implemented the repertoire of known bacteria of the human urinary tract.
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Anaerococcus marasmi sp nov a new bacterium isolated from the human gut microbiota
new microbes and new infections, 2020Co-Authors: Mamadou Lamine Tall, Sara Bellali, Issa Isaac Ngom, Thiphuongthao Pham, Cheikh Ibrahima Lo, Pierre-edouard Fournier, Jeremy Delerce, Anthony LevasseurAbstract:Abstract Anaerococcus marasmi sp. nov. strain Marseille-P3557T is a new species isolated from a stool of a Nigerian child with marasmus. The genome of Marseille-P3557T was 2,130,060 bp-long (35.4% G+C content). The closest species based on 16S rRNA sequence was Anaerococcus prevotii strain 20548 with 97.6% sequence similarity. Considering phenotypic features and comparative genome studies, we proposed the strain Marseille-P3557T as the type strain of Anaerococcus marasmi sp. nov. a new species within the genus Anaerococcus.
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'Anaerococcus mediterraneensis' sp. nov., a new species isolated from human female genital tract.
New microbes and new infections, 2017Co-Authors: K. Diop, Pierre-edouard Fournier, Florence Bretelle, Florence FenollarAbstract:We report the principal characteristics of ‘Anaerococcus mediterraneensis’ strain Marseille P2765, a new member of the Anaerococcus genus. Strain Marseille P2765 was isolated in a vaginal sample of a 26-year-old patient with bacterial vaginosis.
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Anaerococcus urinomassiliensis sp nov isolated from a urine sample of a 17 year old boy affected by autoimmune hepatitis and membranoproliferative glomerulonephritis
new microbes and new infections, 2016Co-Authors: Pierre-edouard Fournier, Jean Christophe Lagier, Aurelie Morand, F Cornu, Michel Tsimaratos, Frederic CadoretAbstract:We report the main characteristics of 'Anaerococcus urinomassiliensis' strain FC4(T) (CSURP2143) that was isolated from a urine sample of a 17-year-old boy affected by autoimmune hepatitis and membranoproliferative glomerulonephritis.
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Anaerococcus rubiinfantis sp nov., isolated from the gut microbiota of a Senegalese infant with severe acute malnutrition
Anaerobe, 2016Co-Authors: Maryam Tidjani Alou, Pierre-edouard Fournier, Saber Khelaifia, Caroline Michelle, Claudia Andrieu, Nicholas Armstrong, Fadi Bittar, Cheikh Sokhna, Aldiouma Diallo, Didier RaoultAbstract:Anaerococcus rubiinfantis sp. nov. strain mt16(T) is a new species within the genus Anaerococcus, which was isolated by the culturomics approach from the gut microbiota of an infant suffering from kwashiorkor. A phenotypic, biochemical and proteomic description of this strain is hereby presented alongside a complete annotation of its genome. This strictly anaerobic species forms Gram-positive non-sporeforming cocci. The major fatty acid was hexadecanoic acid. The phylogenetic analysis of strain mt16(T) showed a 97.9% similarity level with Anaerococcus vaginalis, the closest validly published species. Its genome is 1,929,161 bp long with 29.5% G + C content and contains 1808 protein-coding genes and 56 RNA genes, among which are six rRNA genes. Genomic analysis identified 41/1864 coding genes as ORFans (2.2%) and at least 620/1808 (34.9%) orthologous proteins which are not shared with the closest phylogenetic species. We believe that the extension of the human anaerobic gut compendium by culturomics is one of the first steps that will improve the understanding of the links between the microbiome and health or disease. (C) 2016 Elsevier Ltd. All rights reserved.
Florence Fenollar - One of the best experts on this subject based on the ideXlab platform.
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megasphaera vaginalis sp nov and Anaerococcus vaginimassiliensis sp nov new bacteria isolated from vagina of french woman with bacterial vaginosis
new microbes and new infections, 2020Co-Authors: Alexia Bordigoni, E K Yimagou, Cheikh Ibrahima Lo, Khoudia Diop, Berangere Nicaise, Christelle Desnues, Florence FenollarAbstract:Abstract Using the culturomics method, two strains were isolated, identified and characterized following the taxonogenomics concept. Megasphaera vaginalis sp. nov. strain Marseille-P4512 (= CSURP4512) and Anaerococcus vaginimassiliensis sp. nov. strain Marseille-P4857 (= CSURP4857) were isolated from the vagina of a French woman. The phylogenic tree, phenotypic criteria and genomic analysis described here clearly show that these two bacteria are different from previously known bacterial species with standing in nomenclature and new members of Firmicutes phylum.
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'Anaerococcus mediterraneensis' sp. nov., a new species isolated from human female genital tract.
New microbes and new infections, 2017Co-Authors: K. Diop, Pierre-edouard Fournier, Florence Bretelle, Florence FenollarAbstract:We report the principal characteristics of ‘Anaerococcus mediterraneensis’ strain Marseille P2765, a new member of the Anaerococcus genus. Strain Marseille P2765 was isolated in a vaginal sample of a 26-year-old patient with bacterial vaginosis.
Alida Veloo - One of the best experts on this subject based on the ideXlab platform.
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Anaerococcus nagyae sp nov isolated from human clinical specimens
Anaerobe, 2016Co-Authors: Alida Veloo, E D De Vries, Helene Jeanpierre, Van Arie WinkelhoffAbstract:We describe a new Anaerococcus species isolated from human clinical specimens. Analyses of 16S rRNA gene sequences of three strains showed <98% similarity with its closest relative Anaerococcus octavius. Phylogenetically the isolated strains form a cluster and can be differentiated from other species of the genus Anaerococcus based on its phenotypic characteristics and its MALDI-TOF MS profile. We propose the name Anaerococcus nagyae, with A. nagyae DSM101193 (accession number KU043522) as the type strain.
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Anaerococcus nagyae sp. nov., isolated from human clinical specimens.
Anaerobe, 2015Co-Authors: Alida Veloo, E D De Vries, Hélène Jean-pierre, Van Arie WinkelhoffAbstract:We describe a new Anaerococcus species isolated from human clinical specimens. Analyses of 16S rRNA gene sequences of three strains showed
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Anaerococcus degenerii sp. nov., isolated from human clinical specimens
Anaerobe, 2015Co-Authors: Alida Veloo, P. E. Elgersma, Van Arie WinkelhoffAbstract:Four clinical isolates of gram-positive strict anaerobic cocci were isolated from four different human mixed anaerobic infections. The taxonomical status of the four strains could not be established using standard identification techniques. The biochemical features of the strains were established and their taxonomic position was determined using 16S rRNA sequencing. The four strains form a homogeneous phenotypical and genotypical cluster. A new Anaerococcus species is proposed for these isolates: Anaerococcus degenerii sp. nov. The type strain is UMCG-104(T) = DSM29674(T) (accession number AM176528). (C) 2015 Elsevier Ltd. All rights reserved.
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identification of gram positive anaerobic cocci by maldi tof mass spectrometry
Systematic and Applied Microbiology, 2011Co-Authors: Alida Veloo, Marcel Erhard, Martin Welker, G W Welling, John E DegenerAbstract:Gram-positive anaerobic cocci (GPAC) are part of the commensal microbiota of humans and are a phylogenetically heterogeneous group of organisms. To evaluate the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of GPAC, a database was constructed, using reference strains of commonly encountered GPAC and clinical isolates of which the sequence of the 16S rRNA gene was determined. Subsequently, the database was validated by identifying 107 clinical isolates of GPAC. Results were compared with the identifications obtained by 16S sequencing or fluorescent in situ hybridization (FISH). Strains belonging to the same species grouped together, in most cases, by MALDI-TOF MS analyses. Strains with sequence similarities less than 98% to their closest relatives, formed clusters distinct from recognized species in the MALDI-TOF MS dendrogram and, therefore could not be identified. These strains probably represent new species. Only three clinical isolates (2 strains of Finegoldia magna and 1 strain of Anaerococcus vaginalis) could not be identified. For all the other GPAC strains (96/107), reliable identifications were obtained. Therefore, we concluded that MALDI-TOF MS is an excellent tool for the identification of phylogenetically heterogeneous groups of micro-organisms such as GPAC.
Didier Raoult - One of the best experts on this subject based on the ideXlab platform.
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Anaerococcus urinimassiliensis sp nov a new bacterium isolated from human urine
Scientific Reports, 2021Co-Authors: Aurelie Morand, E K Yimagou, Issa Isaac Ngom, Mamadou Lamine Tall, Anthony Levasseur, Jean Christophe Lagier, Didier Raoult, F Cornu, Michel Tsimaratos, Pierre-edouard FournierAbstract:To date there are thirteen species validly assigned to the genus Anaerococcus. Most of the species in this genus are anaerobic and of human origin. Anaerococcus urinimassiliensis sp. nov., strain Marseille-P2143T is member of family Peptoniphilaceae, which was isolated from the urine of a 17-year-old boy affected by autoimmune hepatitis and membranoproliferative glomerulonephritis using the culturomic approach. In the current study, a taxono-genomics method was employed to describe this new species. The strain Marseille-P2143T was gram positive cocci with translucent colonies on blood agar. Its genome was 2,189,509 bp long with a 33.5 mol% G + C content and exhibited 98.48% 16S rRNA similarity with Anaerococcus provencensis strain 9,402,080. When Anaerococcus urinomassiliensis strain Marseill-P2143T is compared with closely related species, the values ranged from 71.23% with A. hydrogenalis strain DSM 7454T (NZ_ABXA01000052.1) to 90.64% with A. provencensis strain 9402080T (NZ_HG003688.1). This strain has implemented the repertoire of known bacteria of the human urinary tract.
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Standards in Genomic Sciences (2013) 8:548-560 DOI:10.4056/sigs.4177252 The Genomic Standards Consortium
2016Co-Authors: Isabelle Pagnier, Didier Raoult, Olivier Croce, Catherine Robert, Bernard La ScolaAbstract:Non-contiguous finished genome sequence and description of Anaerococcus pacaensis sp. nov., a new species of anaerobic bacteriu
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Anaerococcus rubiinfantis sp nov., isolated from the gut microbiota of a Senegalese infant with severe acute malnutrition
Anaerobe, 2016Co-Authors: Maryam Tidjani Alou, Pierre-edouard Fournier, Saber Khelaifia, Caroline Michelle, Claudia Andrieu, Nicholas Armstrong, Fadi Bittar, Cheikh Sokhna, Aldiouma Diallo, Didier RaoultAbstract:Anaerococcus rubiinfantis sp. nov. strain mt16(T) is a new species within the genus Anaerococcus, which was isolated by the culturomics approach from the gut microbiota of an infant suffering from kwashiorkor. A phenotypic, biochemical and proteomic description of this strain is hereby presented alongside a complete annotation of its genome. This strictly anaerobic species forms Gram-positive non-sporeforming cocci. The major fatty acid was hexadecanoic acid. The phylogenetic analysis of strain mt16(T) showed a 97.9% similarity level with Anaerococcus vaginalis, the closest validly published species. Its genome is 1,929,161 bp long with 29.5% G + C content and contains 1808 protein-coding genes and 56 RNA genes, among which are six rRNA genes. Genomic analysis identified 41/1864 coding genes as ORFans (2.2%) and at least 620/1808 (34.9%) orthologous proteins which are not shared with the closest phylogenetic species. We believe that the extension of the human anaerobic gut compendium by culturomics is one of the first steps that will improve the understanding of the links between the microbiome and health or disease. (C) 2016 Elsevier Ltd. All rights reserved.
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Standards in Genomic Sciences (2014) 9:1198-1210 DOI:10.4056/sigs.5501035 The Genomic Standards Consortium Non-contiguous finished genome sequence and description of
2015Co-Authors: Anaerococcus Provenciensis Sp. Nov, Didier Raoult, Isabelle Pagnier, Olivier Croce, Catherine Robert, Bernard La ScolaAbstract:nov., a new species within the genus Anaerococcus. This strain was isolated from a cervical abscess sample. A. provenciensis is a Gram-positive anaerobic cocci. Here, we describe the features of this organism, together with the complete genome sequence and annotation. The 2.26 Mbp long genome contains 2099 protein-coding and 57 RNA genes including 8 rRNA genes and exhibits a G+C content of 33.48%
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Non-contiguous finished genome sequence and description of Anaerococcus provenciensis sp. nov.
Standards in genomic sciences, 2014Co-Authors: Isabelle Pagnier, Didier Raoult, Olivier Croce, Catherine Robert, Bernard La ScolaAbstract:Anaerococcus provenciensis strain 9402080(T) sp. nov. is the type strain of A. provenciensis sp. nov., a new species within the genus Anaerococcus. This strain was isolated from a cervical abscess sample. A. provenciensis is a Gram-positive anaerobic cocci. Here, we describe the features of this organism, together with the complete genome sequence and annotation. The 2.26 Mbp long genome contains 2099 protein-coding and 57 RNA genes including 8 rRNA genes and exhibits a G+C content of 33.48%.
Anthony Levasseur - One of the best experts on this subject based on the ideXlab platform.
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Anaerococcus urinimassiliensis sp nov a new bacterium isolated from human urine
Scientific Reports, 2021Co-Authors: Aurelie Morand, E K Yimagou, Issa Isaac Ngom, Mamadou Lamine Tall, Anthony Levasseur, Jean Christophe Lagier, Didier Raoult, F Cornu, Michel Tsimaratos, Pierre-edouard FournierAbstract:To date there are thirteen species validly assigned to the genus Anaerococcus. Most of the species in this genus are anaerobic and of human origin. Anaerococcus urinimassiliensis sp. nov., strain Marseille-P2143T is member of family Peptoniphilaceae, which was isolated from the urine of a 17-year-old boy affected by autoimmune hepatitis and membranoproliferative glomerulonephritis using the culturomic approach. In the current study, a taxono-genomics method was employed to describe this new species. The strain Marseille-P2143T was gram positive cocci with translucent colonies on blood agar. Its genome was 2,189,509 bp long with a 33.5 mol% G + C content and exhibited 98.48% 16S rRNA similarity with Anaerococcus provencensis strain 9,402,080. When Anaerococcus urinomassiliensis strain Marseill-P2143T is compared with closely related species, the values ranged from 71.23% with A. hydrogenalis strain DSM 7454T (NZ_ABXA01000052.1) to 90.64% with A. provencensis strain 9402080T (NZ_HG003688.1). This strain has implemented the repertoire of known bacteria of the human urinary tract.
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Anaerococcus marasmi sp nov a new bacterium isolated from the human gut microbiota
new microbes and new infections, 2020Co-Authors: Mamadou Lamine Tall, Sara Bellali, Issa Isaac Ngom, Thiphuongthao Pham, Cheikh Ibrahima Lo, Pierre-edouard Fournier, Jeremy Delerce, Anthony LevasseurAbstract:Abstract Anaerococcus marasmi sp. nov. strain Marseille-P3557T is a new species isolated from a stool of a Nigerian child with marasmus. The genome of Marseille-P3557T was 2,130,060 bp-long (35.4% G+C content). The closest species based on 16S rRNA sequence was Anaerococcus prevotii strain 20548 with 97.6% sequence similarity. Considering phenotypic features and comparative genome studies, we proposed the strain Marseille-P3557T as the type strain of Anaerococcus marasmi sp. nov. a new species within the genus Anaerococcus.
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Anaerococcus marasmi sp nov a new bacterium isolated from human gut microbiota
new microbes and new infections, 2020Co-Authors: Mamadou Lamine Tall, Sara Bellali, Issa Isaac Ngom, Thiphuongthao Pham, Cheikh Ibrahima Lo, P E Fournier, Jeremy Delerce, Anthony LevasseurAbstract:Abstract Anaerococcus marasmi sp. nov. strain Marseille-P3557T is a new species isolated from a stool of a Nigerian child with marasmus. The genome of Marseille-P3557T was 2 130 060 bp long (35.4% G + C content). The closest species based on 16S ribosomal RNA sequence was Anaerococcus prevotii strain 20548, with 97.6% sequence similarity. Considering phenotypic features and comparative genome studies, we propose the strain Marseille-P3557T as the type strain of Anaerococcus marasmi sp. nov., a new species within the genus Anaerococcus.