The Experts below are selected from a list of 6528 Experts worldwide ranked by ideXlab platform

Robert F. Massung - One of the best experts on this subject based on the ideXlab platform.

  • Diversity of Anaplasma phagocytophilum strains, USA.
    Emerging infectious diseases, 2009
    Co-Authors: Eric Morissette, Robert F. Massung, Janet E Foley, A. Rick Alleman, Patrick Foley, Anthony F. Barbet
    Abstract:

    We analyzed the structure of the expression site encoding the immunoprotective protein MSP2/P44 from multiple Anaplasma phagocytophilum strains in the United States. The sequence of p44ESup1 had diverged in Ap-variant 1 strains infecting ruminants. In contrast, no differences were detected between A. phagocytophilum strains infecting humans and domestic dogs.

  • Molecular evidence for Anaplasma phagocytophilum in Israel.
    Emerging infectious diseases, 2007
    Co-Authors: Avi Keysary, Robert F. Massung, Moshe Inbar, Arian D. Wallach, Uri Shanas, Kosta Y. Mumcuoglu, Trevor Waner
    Abstract:

    Sequences from the Anaplasma phagocytophilum 16S rRNA gene were detected in 5 ticks representing 3 species (Hyalomma marginatum, Rhipicephalus turanicus, and Boophilus kohlsi) collected from roe deer (Capreolus capreolus) in Mount Carmel, Israel. The sequences were all identical to those of Ap-variant 1 strain.

  • Reservoir competency of goats for Anaplasma phagocytophilum.
    Annals of the New York Academy of Sciences, 2006
    Co-Authors: Robert F. Massung, Michael L. Levin, Nathan J. Miller, Thomas N. Mather
    Abstract:

    The susceptibility of goats to infection by Anaplasma phagocytophilum (A. phagocytophilum) strains Ap-Variant 1 and Ap-ha was assessed by infestation of goats with field-collected Ixodes scapularis (I. scapularis) ticks. Both strains were infectious in the goat model. These results demonstrate that goats can be used in a laboratory setting to propagate A. phagocytophilum. Transmission from an infected goat to a naive goat by I. scapularis tick feeding was used to demonstrate that goats are reservoir-competent for the Ap-Variant 1 strain.

  • Reservoir competency of goats for the Ap-variant 1 strain of Anaplasma phagocytophilum.
    Infection and immunity, 2006
    Co-Authors: Robert F. Massung, Thomas N. Mather, Michael Levin
    Abstract:

    Field-collected ticks were used to infect goats with either Ap-ha, a strain associated with human disease, or a variant strain, Ap-Variant 1, of Anaplasma phagocytophilum. Goats were shown to be competent as a reservoir for Ap-Variant 1, and challenge and immunosuppression studies were used to further examine infection in the goat model.

  • Anaplasma phagocytophilum in White-tailed Deer
    Emerging infectious diseases, 2005
    Co-Authors: Robert F. Massung, Joshua W. Courtney, Shannon L. Hiratzka, Virginia E. Pitzer, Gary Smith, Richard L. Dryden
    Abstract:

    We examined the reservoir potential of white-tailed deer for Anaplasma phagocytophilum. Results suggest that white-tailed deer harbor a variant strain not associated with human infection, but contrary to published reports, whitetailed deer are not a reservoir for strains that cause human disease. These results will affect surveillance studies of vector and reservoir populations.

Hong Yang - One of the best experts on this subject based on the ideXlab platform.

  • Anaplasma phagocytophilum from rodents and sheep china
    Emerging Infectious Diseases, 2010
    Co-Authors: Lin Zhan, Stephen J Dumler, Jia-fu Jiang, Wu-chun Cao, Xiao Ai Zhang, Yun Xi Liu, Wen Yi Zhang, Pan He Zhang, Chang Ling Bian, Hong Yang
    Abstract:

    To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.

  • Anaplasma phagocytophilum in livestock and small rodents
    Veterinary Microbiology, 2010
    Co-Authors: Lin Zhan, Jia-fu Jiang, Wu-chun Cao, Wei Liu, Xiao Ai Zhang, Wen Yi Zhang, Shu-qing Zuo, Zhi-wei Cao, Hong Yang
    Abstract:

    To identify the prevalence of Anaplasma phagocytophilum in both wild rodents and domestic animals and to make clear the genetic characteristics of the agents from different animals in China, a total of 105 livestock and 159 small rodents were analyzed by real-time-PCR and sequence analysis. The prevalence rate was 6.7% (7/105) and 14.5% (23/159), respectively. The nucleotide sequences of 16S rRNA (rrs) from the positive livestock and rodents were identical to each other. The phylogenetic analysis based on partial A. phagocytophilum p44ESup1 gene revealed that A. phagocytophilum identified in this study was placed on a separate clade distinct from those in other continents. These findings indicated A. phagocytophilum in rodents might be able to infect livestock and intensified the threats of anaplasmosis to livestock in the area. Further studies on public health significance of the agent are worth investigation in future.

  • Anaplasma phagocytophilum and Borrelia burgdorferi in rabbits from southeastern China.
    Veterinary parasitology, 2009
    Co-Authors: Lin Zhan, J. Stephen Dumler, Hong Yang, Chen-yi Chu, Shu-qing Zuo, Na Jia, Bao-gui Jiang, Wu-chun Cao
    Abstract:

    A total of 54 wild rabbits captured from southeastern China were examined for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato by polymerase chain reaction (PCR) assays. One and three samples were positive for A. phagocytophilum and B. burgdorferi, respectively. Sequence analyses of PCR products identified a variant of A. phagocytophilum and a B. garinii genotype. This is the first detection of the two tick-borne agents in Chinese rabbits, the role of which in the maintenance of the agents deserve further investigations.

Wu-chun Cao - One of the best experts on this subject based on the ideXlab platform.

  • Dual infection with Anaplasma phagocytophilum and Babesia microti in a Rattus norvegicus, China
    Ticks and tick-borne diseases, 2013
    Co-Authors: Xiao-guang Zhao, Jia-fu Jiang, Yi Sun, Yao-yun Zhang, Wei Liu, Wu-chun Cao
    Abstract:

    Anaplasma phagocytophilum and Babesia microti were isolated from a Rattus norvegicus in the middle east of China through propagation in experimental mice. Their molecular biological characteristics were described. The public health and veterinary significance of the dual infection of the 2 tick-borne agents deserve further investigation.

  • Anaplasma phagocytophilum from rodents and sheep china
    Emerging Infectious Diseases, 2010
    Co-Authors: Lin Zhan, Stephen J Dumler, Jia-fu Jiang, Wu-chun Cao, Xiao Ai Zhang, Yun Xi Liu, Wen Yi Zhang, Pan He Zhang, Chang Ling Bian, Hong Yang
    Abstract:

    To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.

  • Anaplasma phagocytophilum in livestock and small rodents
    Veterinary Microbiology, 2010
    Co-Authors: Lin Zhan, Jia-fu Jiang, Wu-chun Cao, Wei Liu, Xiao Ai Zhang, Wen Yi Zhang, Shu-qing Zuo, Zhi-wei Cao, Hong Yang
    Abstract:

    To identify the prevalence of Anaplasma phagocytophilum in both wild rodents and domestic animals and to make clear the genetic characteristics of the agents from different animals in China, a total of 105 livestock and 159 small rodents were analyzed by real-time-PCR and sequence analysis. The prevalence rate was 6.7% (7/105) and 14.5% (23/159), respectively. The nucleotide sequences of 16S rRNA (rrs) from the positive livestock and rodents were identical to each other. The phylogenetic analysis based on partial A. phagocytophilum p44ESup1 gene revealed that A. phagocytophilum identified in this study was placed on a separate clade distinct from those in other continents. These findings indicated A. phagocytophilum in rodents might be able to infect livestock and intensified the threats of anaplasmosis to livestock in the area. Further studies on public health significance of the agent are worth investigation in future.

  • Anaplasma phagocytophilum and Borrelia burgdorferi in rabbits from southeastern China.
    Veterinary parasitology, 2009
    Co-Authors: Lin Zhan, J. Stephen Dumler, Hong Yang, Chen-yi Chu, Shu-qing Zuo, Na Jia, Bao-gui Jiang, Wu-chun Cao
    Abstract:

    A total of 54 wild rabbits captured from southeastern China were examined for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato by polymerase chain reaction (PCR) assays. One and three samples were positive for A. phagocytophilum and B. burgdorferi, respectively. Sequence analyses of PCR products identified a variant of A. phagocytophilum and a B. garinii genotype. This is the first detection of the two tick-borne agents in Chinese rabbits, the role of which in the maintenance of the agents deserve further investigations.

Lin Zhan - One of the best experts on this subject based on the ideXlab platform.

  • Anaplasma phagocytophilum from rodents and sheep china
    Emerging Infectious Diseases, 2010
    Co-Authors: Lin Zhan, Stephen J Dumler, Jia-fu Jiang, Wu-chun Cao, Xiao Ai Zhang, Yun Xi Liu, Wen Yi Zhang, Pan He Zhang, Chang Ling Bian, Hong Yang
    Abstract:

    To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.

  • Anaplasma phagocytophilum in livestock and small rodents
    Veterinary Microbiology, 2010
    Co-Authors: Lin Zhan, Jia-fu Jiang, Wu-chun Cao, Wei Liu, Xiao Ai Zhang, Wen Yi Zhang, Shu-qing Zuo, Zhi-wei Cao, Hong Yang
    Abstract:

    To identify the prevalence of Anaplasma phagocytophilum in both wild rodents and domestic animals and to make clear the genetic characteristics of the agents from different animals in China, a total of 105 livestock and 159 small rodents were analyzed by real-time-PCR and sequence analysis. The prevalence rate was 6.7% (7/105) and 14.5% (23/159), respectively. The nucleotide sequences of 16S rRNA (rrs) from the positive livestock and rodents were identical to each other. The phylogenetic analysis based on partial A. phagocytophilum p44ESup1 gene revealed that A. phagocytophilum identified in this study was placed on a separate clade distinct from those in other continents. These findings indicated A. phagocytophilum in rodents might be able to infect livestock and intensified the threats of anaplasmosis to livestock in the area. Further studies on public health significance of the agent are worth investigation in future.

  • Anaplasma phagocytophilum and Borrelia burgdorferi in rabbits from southeastern China.
    Veterinary parasitology, 2009
    Co-Authors: Lin Zhan, J. Stephen Dumler, Hong Yang, Chen-yi Chu, Shu-qing Zuo, Na Jia, Bao-gui Jiang, Wu-chun Cao
    Abstract:

    A total of 54 wild rabbits captured from southeastern China were examined for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato by polymerase chain reaction (PCR) assays. One and three samples were positive for A. phagocytophilum and B. burgdorferi, respectively. Sequence analyses of PCR products identified a variant of A. phagocytophilum and a B. garinii genotype. This is the first detection of the two tick-borne agents in Chinese rabbits, the role of which in the maintenance of the agents deserve further investigations.

Richard L. Dryden - One of the best experts on this subject based on the ideXlab platform.

  • Anaplasma phagocytophilum in White-tailed Deer
    Emerging infectious diseases, 2005
    Co-Authors: Robert F. Massung, Joshua W. Courtney, Shannon L. Hiratzka, Virginia E. Pitzer, Gary Smith, Richard L. Dryden
    Abstract:

    We examined the reservoir potential of white-tailed deer for Anaplasma phagocytophilum. Results suggest that white-tailed deer harbor a variant strain not associated with human infection, but contrary to published reports, whitetailed deer are not a reservoir for strains that cause human disease. These results will affect surveillance studies of vector and reservoir populations.