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Alan Giraldo - One of the best experts on this subject based on the ideXlab platform.
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Prevalencia de parásitos nematodos Strongylidos asociados al Caracol africano, Achatina fulica, en el Valle del Cauca, Colombia
2017Co-Authors: Diego Córdoba R, Angie Patiño Montoya, Alan GiraldoAbstract:Objectives . To establish the presence and prevalence of Strongylida nematode parasites in Achatina fulica in the Valle del Cauca, especially of nematodes that are potentially pathogenic for humans. Materials and methods . A. fulica individuals were collected in nine cities of the Valle del Cauca, Colombia. Direct visual examination was used to identify A. fulica parasites. Nematodes were separated from tissue or collected from mucus, washed in saline solution, and fixed in a hot AFA solution. Samples were mounted in glycerine and observed under the microscope. Results . The general nematode parasite prevalence was 35% in 2013. The city with highest prevalence during 2013 was Cartago (60%), following by Buenaventura (42.9%) and Cali (33%), while during 2014 were Cali (30%) and Buenaventura (30%). The Strongylida nematodes registered were classified in three genera: Angiostrongylus (14.7% prevalence), Aelurostrongylus (2.6%),and Strongyluris (2.6%). The city with highest positive records of Angiostrongylus was Cali during 2014 and Aelurostrongylus was Buenaventura during 2013. Strongyluris genus was recorded only in Cali during 2013, with a prevalence of 11%. Of the nine evaluated cities, five has presence of Angiostrongylus . Conclusions . Three genera of Strongylida nematode were recorded associated with A. fulica s specimens in the Valle del Cauca during 2013 and 2014. Therefore, the role that A. fulica and native mollusk species could be playing in the life cycle of these parasites at the local level should not underestimated.
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Prevalencia de parásitos nematodos Strongylidos asociados al Caracol africano, Achatina fulica, en el Valle del Cauca, Colombia
2017Co-Authors: Diego Córdoba R, Angie Patiño Montoya, Alan GiraldoAbstract:Objetivos. Establecer la presencia y prevalencia de parásitos nematodos Strongylida en Achatina fulica en el Valle del Cauca, especialmente los nematodos que son potencialmente patogénicos para los humanos. Materiales y métodos. Individuos de A. fulica fueron colectados en nueve ciudades del Valle del Cauca, Colombia. Los nematodos fueron identificados mediante inspección directa de los individuos, estos se separaron de los tejidos y el mucus, luego, los nematodos se lavaron en solución salina, y fijaron en solución AFA caliente. Se montaron placas en glicerina para observar al microscopio. Resultados. La prevalencia general de parásitos nematodos fue 35% en 2013. La ciudad con mayor prevalencia durante 2013 fue Cartago (60%), seguido de Buenaventura (42.9%) y Cali (33%), mientras que en 2014 fue Cali (30%) y Buenaventura (30%). Los nematodos Strongylida registrados fueron clasificados en tres géneros: Angiostrongylus (prevalencia 14.7%), Aelurostrongylus (2.6%), y Strongyluris (2.6%). La ciudad con la mayor cantidad de positivos para Angiostrongylus fue Cali durante 2014 y Buenaventura con Aelurostorngylus para el 2013. El género Strongyluris solo se reportó en Cali durante 2013, con una prevalencia del 11%. En cinco de las nueves ciudades evaluadas se registró la presencia de Angiostrongylus. Conclusiones. Tres géneros de nematodos Strongylida se reportaron en individuos de A. fulica en el Valle del Cauca durante 2013 y 2014. Por lo tanto, no debe subestimarse el papel de A. fulica y las especies de moluscos nativas en el ciclo de vida de estos parásitos.
Hoi-sen Yong - One of the best experts on this subject based on the ideXlab platform.
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molecular phylogeography of Angiostrongylus cantonensis nematoda angiostrongylidae and genetic relationships with congeners using cytochrome b gene marker
2015Co-Authors: Hoi-sen Yong, Sze-looi Song, Praphathip Eamsobhana, Anchana PrasartvitAbstract:Abstract Angiostrongylus cantonensis is an important emerging zoonotic parasite causing human eosinophilic meningitis (or meningoencephalitis) in many parts of the world. To-date there is only a single study using mitochondrial cytochrome b (CYTB) gene to determine its genetic structure in eight geographical localities in Thailand. The present study examined the molecular phylogeography of this rat lungworm and its phylogenetic relationship with congeners using CYTB gene marker. A total of 15 CYTB haplotypes was found in 37 sequences from 14 geographical localities (covering north, west, east, central and south regions) in Thailand. These CYTB haplotypes were distinct from those of A. cantonensis for China and Hawaii. In Thailand, some CYTB haplotypes appeared to be confined to specific geographical localities. The partial CYTB DNA nucleotide sequences separated unequivocally the A. cantonensis isolates of Thailand, China and Hawaii as well as the congeners Angiostrongylus malaysiensis , A. costaricensis and Angiostrongylus vasorum , with A. malaysiensis grouped with A. cantonensis and A. costaricensis grouped with A. vasorum . Likewise the congeners of Metastrongylus and Onchocerca genera could also be clearly differentiated. The present study added two new definitive hosts ( Bandicota savilei and Rattus losea ) and three new localities (Mae Hong Son in the north, Tak in the west, and Phang Nga in the south) for A. malaysiensis in Thailand, indicating its wide occurrence in the country. Three CYTB haplotypes were found in the Thailand samples of A. malaysiensis . In addition to differentiation of congeners, CYTB gene marker could be used for determining the genetic diversity of a given population/taxon.
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phylogenetics and systematics of Angiostrongylus lungworms and related taxa nematoda metastrongyloidea inferred from the nuclear small subunit ssu ribosomal dna sequences
2015Co-Authors: Praphathip Eamsobhana, Phaik-eem Lim, Hoi-sen YongAbstract:The Angiostrongylus lungworms are of public health and veterinary concern in many countries. At the family level, the Angiostrongylus lungworms have been included in the family Angiostrongylidae or the family Metastrongylidae. The present study was undertaken to determine the usefulness and suitability of the nuclear 18S (small subunit, SSU) rDNA sequences for differentiating various taxa of the genus Angiostrongylus, as well as to determine the systematics and phylogenetic relationship of Angiostrongylus species and other metastrongyloid taxa. This study revealed six 18S (SSU) haplotypes in A. cantonensis, indicating considerable genetic diversity. The uncorrected pairwise 'p' distances among A. cantonensis ranged from 0 to 0.86%. The 18S (SSU) rDNA sequences unequivocally distinguished the five Angiostrongylus species, confirmed the close relationship of A. cantonensis and A. malaysiensis and that of A. costaricensis and A. dujardini, and were consistent with the family status of Angiostrongylidae and Metastrongylidae. In all cases, the congeneric metastrongyloid species clustered together. There was no supporting evidence to include the genus Skrjabingylus as a member of Metastrongylidae. The genera Aelurostrongylus and Didelphostrongylus were not recovered with Angiostrongylus, indicating polyphyly of the Angiostrongylidae. Of the currently recognized families of Metastrongyloidea, only Crenosomatidae appeared to be monophyletic. In view of the unsettled questions regarding the phylogenetic relationships of various taxa of the metastrongyloid worms, further analyses using more markers and more taxa are warranted.
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Gene order of mitochondrial genome of Angiostrongylus costaricensis (Costa Rica taxon).
2015Co-Authors: Hoi-sen Yong, Sze-looi Song, Praphathip Eamsobhana, Share-yuan Goh, Phaik-eem Lim, Wan-loo Chow, Kok-gan Chan, Elizabeth Abrahams-sandiAbstract:Gene order of mitochondrial genome of Angiostrongylus costaricensis (Costa Rica taxon).
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Start and stop codons and size of mitochondrial protein-coding genes of Brazil (NC_013067) and Costa Rica taxa of Angiostrongylus costaricensis.
2015Co-Authors: Hoi-sen Yong, Sze-looi Song, Praphathip Eamsobhana, Share-yuan Goh, Phaik-eem Lim, Wan-loo Chow, Kok-gan Chan, Elizabeth Abrahams-sandiAbstract:Start and stop codons and size of mitochondrial protein-coding genes of Brazil (NC_013067) and Costa Rica taxa of Angiostrongylus costaricensis.
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molecular differentiation and phylogenetic relationships of three Angiostrongylus species and Angiostrongylus cantonensis geographical isolates based on a 66 kda protein gene of a cantonensis nematoda angiostrongylidae
2010Co-Authors: Praphathip Eamsobhana, Hongman Zhang, Hoi-sen YongAbstract:The phylogenetic relationships and molecular differentiation of three species of angiostrongylid nematodes (Angiostrongylus cantonensis, Angiostrongylus costaricensis and Angiostrongylus malaysiensis) were studied using the AC primers for a 66-kDa protein gene of A. cantonensis. The AC primers successfully amplified the genomic DNA of these angiostrongylid nematodes. No amplification was detected for the DNA of Ascaris lumbricoides, Ascaris suum, Anisakis simplex, Gnathostoma spinigerum, Toxocara canis, and Trichinella spiralis. The maximum-parsimony (MP) consensus tree and the maximum-likelihood (ML) tree both showed that the Angiostrongylus taxa could be divided into two major clades – Clade 1 (A. costaricensis) and Clade 2 (A. cantonensis and A. malaysiensis) with a full support bootstrap value. A. costaricensis is the most distant taxon. A. cantonensis is a sister group to A. malaysiensis; these two taxa (species) are clearly separated. There is no clear distinction between the A. cantonensis samples from four different geographical localities (Thailand, China, Japan and Hawaii); only some of the samples are grouped ranging from no support or low support to moderate support of bootstrap values. The published nucleotide sequences of A. cantonensis adult-specific native 66 kDa protein mRNA, clone L5–400 from Taiwan (U17585) appear to be very distant from the A. cantonensis samples from Thailand, China, Japan and Hawaii, with the uncorrected p-distance values ranging from 26.87% to 29.92%.
Jun-ichi Miyazaki - One of the best experts on this subject based on the ideXlab platform.
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defective b 1 cell development and impaired immunity against Angiostrongylus cantonensis in il 5rα deficient mice
1996Co-Authors: Toshimi Yoshida, Hiroko Sugaya, Kazushige Maki, Hiroko Kanazawa, Shinji Sunaga, Kentarou Yoshimura, Tatsuo Kinashi, M. Takagi, Koichi Ikuta, Jun-ichi MiyazakiAbstract:Abstract We generated interleukin-5 receptor α chain (IL-5Rα)–deficient (IL-5Rα −/− ) mice by gene targeting. The IL-5Rα −/− mice showed decreased numbers of B-1 cells concomitant with low serum concentrations of IgM and IgG3. They showed no IL-5-induced enhancement of B cell responses to T-independent antigens. The number of αβ T cell receptor–positive thymocytes tended to decrease in 3-week-old IL-5Rα −/− mice, returning to normal by 6 weeks of age. The IL-5Rα −/− mice produced basal levels of eosinophils, while their bone marrow cells failed to form eosinophilic colonies in response to IL-5. Impaired eosinophilopoiesis in IL-5Rα −/− mice enhanced the survival of Angiostrongylus cantonensis. These results indicate that IL-5-induced eosinophils serve as potent effector cells in the killing of Angiostrongylus cantonensis in mice.
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defective b 1 cell development and impaired immunity against Angiostrongylus cantonensis in il 5rα deficient mice
1996Co-Authors: Toshimi Yoshida, Hiroko Sugaya, Kazushige Maki, Hiroko Kanazawa, Shinji Sunaga, Kentarou Yoshimura, Tatsuo Kinashi, M. Takagi, Koichi Ikuta, Jun-ichi MiyazakiAbstract:We generated interleukin-5 receptor alpha chain (IL-5R alpha)-deficient (IL-5R alpha-/-) mice by gene targeting. The IL-5R alpha-/- mice showed decreased numbers of B-1 cells concomitant with low serum concentrations of IgM and IgG3. They showed no IL-5-induced enhancement of B cell responses to T-independent antigens. The number of alpha beta T cell receptor-positive thymocytes tended to decrease in 3-week-old IL-5R alpha-/- mice, returning to normal by 6 weeks of age. The IL-5R alpha-/- mice produced basal levels of eosinophils, while their bone marrow cells failed to form eosinophilic colonies in response to IL-5. Impaired eosinophilopoiesis in IL-5R alpha-/-mice enhanced the survival of Angiostrongylus cantonensis. These results indicate that IL-5-induced eosinophils serve as potent effector cells in the killing of Angiostrongylus cantonensis in mice.
Jenny Chaparro J. Gutiérrez - One of the best experts on this subject based on the ideXlab platform.
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Mono- and co-infections with Aelurostrongylus abstrusus, Angiostrongylus vasorum, Crenosoma vulpis and Troglostrongylus brevior, unknown Aelurostrongylus sp. and Angiostrongylus sp.
2019Co-Authors: Felipe Penagos-tabares, Carlos Hermosilla, Anja Taubert, Malin K. Lange, Juan Vélez, Jörg Hirzmann, Jesed Gutiérrez-arboleda, Jenny Chaparro J. GutiérrezAbstract:Mono- and co-infections with Aelurostrongylus abstrusus, Angiostrongylus vasorum, Crenosoma vulpis and Troglostrongylus brevior, unknown Aelurostrongylus sp. and Angiostrongylus sp.
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The invasive giant African snail Lissachatina fulica as natural intermediate host of Aelurostrongylus abstrusus, Angiostrongylus vasorum, Troglostrongylus brevior, and Crenosoma vulpis in Colombia
2019Co-Authors: Felipe Penagos-tabares, Carlos Hermosilla, Anja Taubert, Malin K. Lange, Juan Vélez, Jörg Hirzmann, Jesed Gutiérrez-arboleda, Jenny Chaparro J. GutiérrezAbstract:BackgroundSeveral metastrongyloid lungworms are unreported pathogens in Colombia. Angiostrongylus vasorum and Crenosoma vulpis target the cardiopulmonary system of domestic and wild canids. Aelurostrongylus abstrusus and Troglostrongylus brevior infect felids and considering that six wild felid species exist in Colombia, knowledge of feline lungworm infections is important for their conservation. The zoonotic metastrongyloids Angiostrongylus costaricensis and Angiostrongylus cantonensis can cause severe gastrointestinal and neurological diseases. Angiostrongylus costaricensis has been reported in Colombia, while Ang. cantonensis is present in neighbouring countries. Research on the epidemiology of metastrongyloids in Colombia and South America more broadly requires evaluating the role that gastropods play as intermediate hosts in their life cycles. This study assessed the prevalence of metastrongyloid larvae in populations of the invasive giant African snail, Lissachatina fulica, in Colombia.Methodology/Principal findingsA total of 609 Lissachantina fulica were collected from 6 Colombian municipalities. The snails were then cryo-euthanized, artificially digested and the sediments examined microscopically for the presence of metastrongyloid larvae. Based on morphological characteristics 53.3% (56/107) of the snails from Puerto Leguízamo (Department of Putumayo) were infected with Ael. abstrusus larvae, 8.4% (9/107) with Ang. vasorum larvae, 6.5% (7/107) with T. brevior larvae and 5.6% (6/107) with C. vulpis larvae, being the region with highest prevalences of the four species. Snails from Andes (Department of Antioquia) and Tulúa (Department of Valle del Cauca) were positive for Ang. vasorum larvae with a prevalence of 4.6 (11/238) and 6.3% (4/64), respectively. Species identifications were confirmed by PCR and sequencing.Conclusions/SignificanceThis epidemiological survey reports for first time the presence of Ael. abstrusus, T. brevior, C. vulpis and Ang. vasorum in L. fulica in a number of regions of Colombia.
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Larval burden categories for Aelurostrongylus abstrusus, Angiostrongylus vasorum, Crenosoma vulpis, Troglostrongylus brevior and unknown Aelurostrongylus sp. per infection.
2019Co-Authors: Felipe Penagos-tabares, Carlos Hermosilla, Anja Taubert, Malin K. Lange, Juan Vélez, Jörg Hirzmann, Jesed Gutiérrez-arboleda, Jenny Chaparro J. GutiérrezAbstract:In this graphic are referred 96 infections, 2 infections of unknown Angiostrongylus spp. were not included. Species identification was confirmed via PCR and corroborate by sequencing.
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Metastrongyloid larvae found in Lissachatina fulica from different regions of Colombia.
2019Co-Authors: Felipe Penagos-tabares, Carlos Hermosilla, Anja Taubert, Malin K. Lange, Juan Vélez, Jörg Hirzmann, Jesed Gutiérrez-arboleda, Jenny Chaparro J. GutiérrezAbstract:(A) Aelurostrongylus abstrusus L1, scale bar 20 μm. (B) Troglostrongylus brevior L1, scale bar 50 μm. (C) Crenosoma vulpis L1, scale bar 50 μm. (D) Angiostrongylus vasorum L1, scale bar 50 μm.
Diego Córdoba R - One of the best experts on this subject based on the ideXlab platform.
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Prevalencia de parásitos nematodos Strongylidos asociados al Caracol africano, Achatina fulica, en el Valle del Cauca, Colombia
2017Co-Authors: Diego Córdoba R, Angie Patiño Montoya, Alan GiraldoAbstract:Objectives . To establish the presence and prevalence of Strongylida nematode parasites in Achatina fulica in the Valle del Cauca, especially of nematodes that are potentially pathogenic for humans. Materials and methods . A. fulica individuals were collected in nine cities of the Valle del Cauca, Colombia. Direct visual examination was used to identify A. fulica parasites. Nematodes were separated from tissue or collected from mucus, washed in saline solution, and fixed in a hot AFA solution. Samples were mounted in glycerine and observed under the microscope. Results . The general nematode parasite prevalence was 35% in 2013. The city with highest prevalence during 2013 was Cartago (60%), following by Buenaventura (42.9%) and Cali (33%), while during 2014 were Cali (30%) and Buenaventura (30%). The Strongylida nematodes registered were classified in three genera: Angiostrongylus (14.7% prevalence), Aelurostrongylus (2.6%),and Strongyluris (2.6%). The city with highest positive records of Angiostrongylus was Cali during 2014 and Aelurostrongylus was Buenaventura during 2013. Strongyluris genus was recorded only in Cali during 2013, with a prevalence of 11%. Of the nine evaluated cities, five has presence of Angiostrongylus . Conclusions . Three genera of Strongylida nematode were recorded associated with A. fulica s specimens in the Valle del Cauca during 2013 and 2014. Therefore, the role that A. fulica and native mollusk species could be playing in the life cycle of these parasites at the local level should not underestimated.
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Prevalencia de parásitos nematodos Strongylidos asociados al Caracol africano, Achatina fulica, en el Valle del Cauca, Colombia
2017Co-Authors: Diego Córdoba R, Angie Patiño Montoya, Alan GiraldoAbstract:Objetivos. Establecer la presencia y prevalencia de parásitos nematodos Strongylida en Achatina fulica en el Valle del Cauca, especialmente los nematodos que son potencialmente patogénicos para los humanos. Materiales y métodos. Individuos de A. fulica fueron colectados en nueve ciudades del Valle del Cauca, Colombia. Los nematodos fueron identificados mediante inspección directa de los individuos, estos se separaron de los tejidos y el mucus, luego, los nematodos se lavaron en solución salina, y fijaron en solución AFA caliente. Se montaron placas en glicerina para observar al microscopio. Resultados. La prevalencia general de parásitos nematodos fue 35% en 2013. La ciudad con mayor prevalencia durante 2013 fue Cartago (60%), seguido de Buenaventura (42.9%) y Cali (33%), mientras que en 2014 fue Cali (30%) y Buenaventura (30%). Los nematodos Strongylida registrados fueron clasificados en tres géneros: Angiostrongylus (prevalencia 14.7%), Aelurostrongylus (2.6%), y Strongyluris (2.6%). La ciudad con la mayor cantidad de positivos para Angiostrongylus fue Cali durante 2014 y Buenaventura con Aelurostorngylus para el 2013. El género Strongyluris solo se reportó en Cali durante 2013, con una prevalencia del 11%. En cinco de las nueves ciudades evaluadas se registró la presencia de Angiostrongylus. Conclusiones. Tres géneros de nematodos Strongylida se reportaron en individuos de A. fulica en el Valle del Cauca durante 2013 y 2014. Por lo tanto, no debe subestimarse el papel de A. fulica y las especies de moluscos nativas en el ciclo de vida de estos parásitos.
