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Mustafa Numan Bucak - One of the best experts on this subject based on the ideXlab platform.
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the effect of antioxidants on post thawed Angora Goat capra hircus ancryrensis sperm parameters lipid peroxidation and antioxidant activities
Small Ruminant Research, 2010Co-Authors: Mustafa Numan Bucak, Ahmet Atessahin, Pürhan Barbaros Tuncer, Serpil Sariozkan, Fatih Sakin, Recai Kulaksiz, Mesut CevikAbstract:Abstract The aim of this study was to determine the effects of the antioxidants curcumin, inositol and carnitine on microscopic seminal parameters, lipid peroxidation (LPO) and the antioxidant activities of sperm, following the freeze-thawing of Angora Goat semen. Ejaculates were collected via artificial vagina from three Angora Goats and microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including curcumin (2.5, 5 or 10 mM), inositol (2.5, 5 or 10 mM), carnitine (2.5, 5 or 10 mM) and no antioxidant (control). The diluted semen was slowly (at a rate of 0.2–0.3 °C/min) cooled to 5 °C and then cryopreserved in 0.25 mL French straws. Frozen straws were thawed individually at 37 °C for 20 s in a water bath, for microscopic sperm evaluation. The freezing extender supplemented with 2.5 mM curcumin led to higher percentage of computer-assisted semen analyzer (CASA) sperm motility (65 ± 3%), when compared to the control, inositol and the 10 mM carnitine ( P P
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effects of hypotaurine cysteamine and aminoacids solution on post thaw microscopic and oxidative stress parameters of Angora Goat semen
Research in Veterinary Science, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Kenan Coyan, Nuri Baspinar, Birol OzkalpAbstract:Abstract This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora Goat semen after the freeze–thawing process. Ejaculates collected from four Angora Goats were evaluated and pooled at 37 °C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 °C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 °C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant ( P P P P > 0.05). The antioxidants hypotaurine and cysteamine decreased SOD activity when compared to the BME group and controls ( P
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effect of antioxidants on microscopic semen parameters lipid peroxidation and antioxidant activities in Angora Goat semen following cryopreservation
Small Ruminant Research, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Halil Ibrahim AkcadagAbstract:Abstract The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze–thawing of Angora Goat semen. Ejaculates collected from three Angora Goat bucks, were evaluated and pooled at 37 °C. The semen samples which were diluted with a Tris-based extender containing additives including glutamine (2.5; 5 mM) and hyaluronan (500; 1000 μl/ml), and an extender containing no antioxidants (control) were cooled to 5 °C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were thawed individually (37 °C) for 20 s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5 mM glutamine led to higher sperm motility and hypo-osmotic swelling test (HOST) values, compared to the control ( P P P
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Effect of antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora Goat semen following cryopreservation
'Elsevier BV', 2009Co-Authors: Mustafa Numan Bucak, Ulutas, Pinar Alkim, Tuncer, Puerhan Barbaros, SariÖzkan Serpil, Halil Ibrahim AkcadagAbstract:The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze-thawing of Angora Goat semen. Ejaculates collected front three Angora Goat bucks, were evaluated and pooled at 37 degrees C. The semen samples which were diluted with a Tris-based extender containing additives including glutamine (2.5:5 mM) and hyaluronan (500; 1000 mu l/ml), and an extender containing no antioxidants (control) were cooled to 5 C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were thawed individually (37 degrees C) for 20s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5 mM glutamine led to higher sperm motility and hypo-osmotic swelling test (HOST) values, compared to the control (P
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Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora Goat semen
'Elsevier BV', 2009Co-Authors: Mustafa Numan Bucak, SariÖzkan Serpil, Pb Tuncer, Pa Ulutaş, Çoyan K, Başpınar N, Özkalp BAbstract:This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora Goat semen after the freeze-thawing process. Ejaculates collected from four Angora Goats were evaluated and pooled at 37 degrees C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 degrees C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 degrees C for 20 s in a water bath for evaluation
Pürhan Barbaros Tuncer - One of the best experts on this subject based on the ideXlab platform.
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the effect of antioxidants on post thawed Angora Goat capra hircus ancryrensis sperm parameters lipid peroxidation and antioxidant activities
Small Ruminant Research, 2010Co-Authors: Mustafa Numan Bucak, Ahmet Atessahin, Pürhan Barbaros Tuncer, Serpil Sariozkan, Fatih Sakin, Recai Kulaksiz, Mesut CevikAbstract:Abstract The aim of this study was to determine the effects of the antioxidants curcumin, inositol and carnitine on microscopic seminal parameters, lipid peroxidation (LPO) and the antioxidant activities of sperm, following the freeze-thawing of Angora Goat semen. Ejaculates were collected via artificial vagina from three Angora Goats and microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including curcumin (2.5, 5 or 10 mM), inositol (2.5, 5 or 10 mM), carnitine (2.5, 5 or 10 mM) and no antioxidant (control). The diluted semen was slowly (at a rate of 0.2–0.3 °C/min) cooled to 5 °C and then cryopreserved in 0.25 mL French straws. Frozen straws were thawed individually at 37 °C for 20 s in a water bath, for microscopic sperm evaluation. The freezing extender supplemented with 2.5 mM curcumin led to higher percentage of computer-assisted semen analyzer (CASA) sperm motility (65 ± 3%), when compared to the control, inositol and the 10 mM carnitine ( P P
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effects of hypotaurine cysteamine and aminoacids solution on post thaw microscopic and oxidative stress parameters of Angora Goat semen
Research in Veterinary Science, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Kenan Coyan, Nuri Baspinar, Birol OzkalpAbstract:Abstract This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora Goat semen after the freeze–thawing process. Ejaculates collected from four Angora Goats were evaluated and pooled at 37 °C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 °C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 °C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant ( P P P P > 0.05). The antioxidants hypotaurine and cysteamine decreased SOD activity when compared to the BME group and controls ( P
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effect of antioxidants on microscopic semen parameters lipid peroxidation and antioxidant activities in Angora Goat semen following cryopreservation
Small Ruminant Research, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Halil Ibrahim AkcadagAbstract:Abstract The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze–thawing of Angora Goat semen. Ejaculates collected from three Angora Goat bucks, were evaluated and pooled at 37 °C. The semen samples which were diluted with a Tris-based extender containing additives including glutamine (2.5; 5 mM) and hyaluronan (500; 1000 μl/ml), and an extender containing no antioxidants (control) were cooled to 5 °C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were thawed individually (37 °C) for 20 s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5 mM glutamine led to higher sperm motility and hypo-osmotic swelling test (HOST) values, compared to the control ( P P P
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effects of anti oxidant additives on microscopic and oxidative parameters of Angora Goat semen following the freeze thawing process
Small Ruminant Research, 2008Co-Authors: Ahmet Atessahin, Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Meltem KizilAbstract:Abstract The anti-oxidant system of reduced glutathione (GSH), glutathione peroxidase (GSH-PX), catalase (CAT), and superoxide dismutase (SOD) has been described as a defense functioning mechanism against lipid peroxidation (LPO) in semen, and is important in maintaining sperm motility and viability. This anti-oxidant capacity of sperm cells may be insufficient in preventing LPO during the freeze–thawing process. The aim of this study was thus to determine the influence of varying doses of anti-oxidant additives on standard semen parameters, lipid peroxidation and anti-oxidant activities after the freeze–thawing of Goat semen. Ejaculate samples (artificial vagina) obtained from 4 mature Angora Goats were evaluated and pooled at 37 °C. The semen samples diluted with a Tris-based extender, containing taurine (25, 50, 75 mM), trehalose (25, 50, 75 mM), and cysteine (5, 10, 15 mM), and an extender containing no anti-oxidant additives (control) were again evaluated. Diluted semen was cooled down to 5 °C and frozen in 0.25 ml French straws, prior to being stored in liquid nitrogen. Frozen straws were thawed in a water bath (37 °C) for 30 s for microscopic sperm evaluation. Upon evaluation of parameters for semen quality, the use of a Tris-based extender supplemented with anti-oxidant additives was found to cause no significant improvement in sperm mortality, when compared to the controls. Increasing doses of taurine and trehalose decreased ( P P P P P P
Serpil Sariozkan - One of the best experts on this subject based on the ideXlab platform.
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the effect of antioxidants on post thawed Angora Goat capra hircus ancryrensis sperm parameters lipid peroxidation and antioxidant activities
Small Ruminant Research, 2010Co-Authors: Mustafa Numan Bucak, Ahmet Atessahin, Pürhan Barbaros Tuncer, Serpil Sariozkan, Fatih Sakin, Recai Kulaksiz, Mesut CevikAbstract:Abstract The aim of this study was to determine the effects of the antioxidants curcumin, inositol and carnitine on microscopic seminal parameters, lipid peroxidation (LPO) and the antioxidant activities of sperm, following the freeze-thawing of Angora Goat semen. Ejaculates were collected via artificial vagina from three Angora Goats and microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including curcumin (2.5, 5 or 10 mM), inositol (2.5, 5 or 10 mM), carnitine (2.5, 5 or 10 mM) and no antioxidant (control). The diluted semen was slowly (at a rate of 0.2–0.3 °C/min) cooled to 5 °C and then cryopreserved in 0.25 mL French straws. Frozen straws were thawed individually at 37 °C for 20 s in a water bath, for microscopic sperm evaluation. The freezing extender supplemented with 2.5 mM curcumin led to higher percentage of computer-assisted semen analyzer (CASA) sperm motility (65 ± 3%), when compared to the control, inositol and the 10 mM carnitine ( P P
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effects of hypotaurine cysteamine and aminoacids solution on post thaw microscopic and oxidative stress parameters of Angora Goat semen
Research in Veterinary Science, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Kenan Coyan, Nuri Baspinar, Birol OzkalpAbstract:Abstract This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora Goat semen after the freeze–thawing process. Ejaculates collected from four Angora Goats were evaluated and pooled at 37 °C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 °C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 °C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant ( P P P P > 0.05). The antioxidants hypotaurine and cysteamine decreased SOD activity when compared to the BME group and controls ( P
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effect of antioxidants on microscopic semen parameters lipid peroxidation and antioxidant activities in Angora Goat semen following cryopreservation
Small Ruminant Research, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Halil Ibrahim AkcadagAbstract:Abstract The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze–thawing of Angora Goat semen. Ejaculates collected from three Angora Goat bucks, were evaluated and pooled at 37 °C. The semen samples which were diluted with a Tris-based extender containing additives including glutamine (2.5; 5 mM) and hyaluronan (500; 1000 μl/ml), and an extender containing no antioxidants (control) were cooled to 5 °C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were thawed individually (37 °C) for 20 s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5 mM glutamine led to higher sperm motility and hypo-osmotic swelling test (HOST) values, compared to the control ( P P P
Birol Ozkalp - One of the best experts on this subject based on the ideXlab platform.
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effects of hypotaurine cysteamine and aminoacids solution on post thaw microscopic and oxidative stress parameters of Angora Goat semen
Research in Veterinary Science, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Kenan Coyan, Nuri Baspinar, Birol OzkalpAbstract:Abstract This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora Goat semen after the freeze–thawing process. Ejaculates collected from four Angora Goats were evaluated and pooled at 37 °C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 °C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 °C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant ( P P P P > 0.05). The antioxidants hypotaurine and cysteamine decreased SOD activity when compared to the BME group and controls ( P
Pinar Alkim Ulutas - One of the best experts on this subject based on the ideXlab platform.
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effects of hypotaurine cysteamine and aminoacids solution on post thaw microscopic and oxidative stress parameters of Angora Goat semen
Research in Veterinary Science, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Kenan Coyan, Nuri Baspinar, Birol OzkalpAbstract:Abstract This study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora Goat semen after the freeze–thawing process. Ejaculates collected from four Angora Goats were evaluated and pooled at 37 °C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 °C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 °C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant ( P P P P > 0.05). The antioxidants hypotaurine and cysteamine decreased SOD activity when compared to the BME group and controls ( P
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effect of antioxidants on microscopic semen parameters lipid peroxidation and antioxidant activities in Angora Goat semen following cryopreservation
Small Ruminant Research, 2009Co-Authors: Mustafa Numan Bucak, Pürhan Barbaros Tuncer, Serpil Sariozkan, Pinar Alkim Ulutas, Halil Ibrahim AkcadagAbstract:Abstract The aim of this study was to determine the effects of the antioxidants glutamine and hyaluronan and the inclusion of different levels on microscopic semen parameters, lipid peroxidation and the antioxidant activities following the freeze–thawing of Angora Goat semen. Ejaculates collected from three Angora Goat bucks, were evaluated and pooled at 37 °C. The semen samples which were diluted with a Tris-based extender containing additives including glutamine (2.5; 5 mM) and hyaluronan (500; 1000 μl/ml), and an extender containing no antioxidants (control) were cooled to 5 °C and frozen in 0.25 ml French straws and stored in liquid nitrogen. Frozen straws were thawed individually (37 °C) for 20 s in a water bath for microscopic evaluation. Freezing extenders supplemented with 2.5 and 5 mM glutamine led to higher sperm motility and hypo-osmotic swelling test (HOST) values, compared to the control ( P P P