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Christoph Lämmler - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of Arcanobacterium phocisimile strain dsm 26142 isolated from a harbor seal
Microbiology Resource Announcements, 2021Co-Authors: Maria Borowiak, Mazen Alssahen, Christoph Lämmler, Burkhard Malorny, Ursula Siebert, Madeleine Plotz, Amir AbdulmawjoodAbstract:Bacteria of the genus Arcanobacterium can be found in a variety of hosts. The species Arcanobacterium phocisimile was originally identified in a free-living harbor seal in the German North Sea in 2004. Here, we announce the complete genome sequence of Arcanobacterium phocisimile strain DSM 26142.
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Arcanobacterium bovis sp nov isolated from the milk of a cow with mastitis
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Osama Sammra, Mazen Alssahen, Christoph Lämmler, A. A. Hassan, Peter Kampfer, Stefanie P Glaeser, Hansjurgen Busse, Geoffrey Foster, Maria Borowiak, Burkhard MalornyAbstract:A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium designated strain C605018/01/1T isolated from a milk sample collected from the udder of a cow at post mortem. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strain of Arcanobacterium pluranimalium (99.76 %); sequence similarities to all other Arcanobacterium species were below 97 %. The wet-lab DNA-DNA hybridization values among strain C605018/01/1T and A. pluranimalium DSM 13483ᵀ were low, 16.9 % (reciprocal, 49.8 %). Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01T displayed a G+C content of 51.6 % mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and unidentified glycolipid and aminophospholipids. Based on these results it is proposed that strain C605018/01/1T should be classified as representing a novel species, Arcanbacterium bovis sp. nov. The type strain C605018/01/1T (CCUG 45425T=DSM 107286T=BCCM/LMG 30783T).
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Identification of Arcanobacterium phocae isolated from fur animals by phenotypic properties, by MALDI-TOF MS analysis and by detection of phocaelysin encoding gene phl as probable novel target.
Veterinary microbiology, 2018Co-Authors: Mazen Alssahen, Osama Sammra, Jörn-peter Wickhorst, Christoph Lämmler, Abdulwahed Ahmed Hassan, Ellen Prenger-berninghoff, Markus Timke, Mirja Raunio Saarnisto, André Becker, Amir AbdulmawjoodAbstract:Abstract In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl . The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002 T and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes . In addition, gene phl could be determined with a newly designed loop-mediated isothermal amplification (LAMP) assay. The detection of mass spectra by MALDI-TOF MS and the LAMP assay based on gene phl might help to reliably identify A. phocae in future and also elucidate the role this species plays in infections of fur animals.
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Arcanobacterium wilhelmae sp nov isolated from the genital tract of a rhinoceros rhinoceros unicornis
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Osama Sammra, Mazen Alssahen, Jörn-peter Wickhorst, Christoph Lämmler, A. A. Hassan, Jorg Rau, Peter Kampfer, Stefanie P Glaeser, Hansjurgen BusseAbstract:A taxonomic study using a polyphasic approach was performed on an unidentified Arcanobacterium -like Gram-stain-positive bacterium isolated from the genital tract of a rhinoceros. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium canis (98.8 % 16S rRNA gene sequence similarity), Arcanobacterium phocisimile (97.8 %), Arcanobacterium phocae (97.7 %), Arcanobacterium haemolyticum (97.4 %), Arcanobacterium hippocoleae (96.6 %), Arcanobacterium pinnipediorum (96.4 %) and Arcarnobacterium pluranimalium (95.4 %). DNA–DNA hybridization values between strain 647T and Arcanobacterium canis DSM 25104T were very low, 13.4 % (reciprocal 15.9 %). The genomic DNA G+C content of strain 647T was 58.7 mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium . The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine and an unidentified phosphoglycolipid. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium . Based on these tests, it is proposed that the unknown bacterium should be classified as a representative of a novel species of the genus Arcanobacterium named Arcanobacterium wilhelmae sp. nov. The type strain is 647T (=DSM 102162T=LMG 29418T).
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Short Communication
2016Co-Authors: H. Ülbegi-mohyla, M. Hijazin, J. Alber, Christoph Lämmler, Ellen Prenger-berninghoff, Amir Abdulmawjood, R. Weiß, A. A. Hassan, Michael ZschöckAbstract:Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic propertie
Amir Abdulmawjood - One of the best experts on this subject based on the ideXlab platform.
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complete genome sequence of Arcanobacterium phocisimile strain dsm 26142 isolated from a harbor seal
Microbiology Resource Announcements, 2021Co-Authors: Maria Borowiak, Mazen Alssahen, Christoph Lämmler, Burkhard Malorny, Ursula Siebert, Madeleine Plotz, Amir AbdulmawjoodAbstract:Bacteria of the genus Arcanobacterium can be found in a variety of hosts. The species Arcanobacterium phocisimile was originally identified in a free-living harbor seal in the German North Sea in 2004. Here, we announce the complete genome sequence of Arcanobacterium phocisimile strain DSM 26142.
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Identification of Arcanobacterium phocae isolated from fur animals by phenotypic properties, by MALDI-TOF MS analysis and by detection of phocaelysin encoding gene phl as probable novel target.
Veterinary microbiology, 2018Co-Authors: Mazen Alssahen, Osama Sammra, Jörn-peter Wickhorst, Christoph Lämmler, Abdulwahed Ahmed Hassan, Ellen Prenger-berninghoff, Markus Timke, Mirja Raunio Saarnisto, André Becker, Amir AbdulmawjoodAbstract:Abstract In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl . The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002 T and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes . In addition, gene phl could be determined with a newly designed loop-mediated isothermal amplification (LAMP) assay. The detection of mass spectra by MALDI-TOF MS and the LAMP assay based on gene phl might help to reliably identify A. phocae in future and also elucidate the role this species plays in infections of fur animals.
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Short Communication
2016Co-Authors: H. Ülbegi-mohyla, M. Hijazin, J. Alber, Christoph Lämmler, Ellen Prenger-berninghoff, Amir Abdulmawjood, R. Weiß, A. A. Hassan, Michael ZschöckAbstract:Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic propertie
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Application of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella pyogenes isolated from various origins
Molecular and cellular probes, 2016Co-Authors: Amir Abdulmawjood, Mazen Alssahen, Osama Sammra, Jörn-peter Wickhorst, Christoph Lämmler, Abdulwahed Ahmed Hassan, Ellen Prenger-berninghoff, O. Hashim, Guenter KleinAbstract:In the present study 28 Trueperella pyogenes strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene cpn60 encoding chaperonin. No cross reaction could be observed with control strains representing four species of genus Trueperella and seven species of closely related genus Arcanobacterium. The present cpn60 LAMP assay might allow a reliable and low cost identification of T. pyogenes also in laboratories with less specified equipment.
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development of a loop mediated isothermal amplification lamp assay for rapid and sensitive identification of Arcanobacterium pluranimalium
Molecular and Cellular Probes, 2015Co-Authors: Amir Abdulmawjood, Osama Sammra, Jörn-peter Wickhorst, Christoph Lämmler, Geoffrey Foster, Ellen Prengerberninghoff, P N Wragg, Guenter KleinAbstract:In the present study 28 Arcanobacterium pluranimalium strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene pla encoding pluranimaliumlysin. No comparable reaction could be observed with control strains representing five species of genus Arcanobacterium and five species of genus Trueperella. The presented pla LAMP assay might allow a reliable and low cost identification of this bacterial pathogen also in laboratories with less specified equipment.
Osama Sammra - One of the best experts on this subject based on the ideXlab platform.
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Arcanobacterium bovis sp nov isolated from the milk of a cow with mastitis
International Journal of Systematic and Evolutionary Microbiology, 2020Co-Authors: Osama Sammra, Mazen Alssahen, Christoph Lämmler, A. A. Hassan, Peter Kampfer, Stefanie P Glaeser, Hansjurgen Busse, Geoffrey Foster, Maria Borowiak, Burkhard MalornyAbstract:A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium designated strain C605018/01/1T isolated from a milk sample collected from the udder of a cow at post mortem. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strain of Arcanobacterium pluranimalium (99.76 %); sequence similarities to all other Arcanobacterium species were below 97 %. The wet-lab DNA-DNA hybridization values among strain C605018/01/1T and A. pluranimalium DSM 13483ᵀ were low, 16.9 % (reciprocal, 49.8 %). Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01T displayed a G+C content of 51.6 % mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and unidentified glycolipid and aminophospholipids. Based on these results it is proposed that strain C605018/01/1T should be classified as representing a novel species, Arcanbacterium bovis sp. nov. The type strain C605018/01/1T (CCUG 45425T=DSM 107286T=BCCM/LMG 30783T).
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Identification of Arcanobacterium phocae isolated from fur animals by phenotypic properties, by MALDI-TOF MS analysis and by detection of phocaelysin encoding gene phl as probable novel target.
Veterinary microbiology, 2018Co-Authors: Mazen Alssahen, Osama Sammra, Jörn-peter Wickhorst, Christoph Lämmler, Abdulwahed Ahmed Hassan, Ellen Prenger-berninghoff, Markus Timke, Mirja Raunio Saarnisto, André Becker, Amir AbdulmawjoodAbstract:Abstract In the present study 12 Arcanobacterium phocae strains isolated from fur animals in Finland, including foxes, minks and Finnraccoons, could successfully be identified phenotypically, by matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and phocaelysin (PHL) encoding gene phl . The PHL of all 12 A. phocae strains in the present study and reference strains A. phocae DSM 10002 T and A. phocae DSM 10003 displayed, as typical members of the cholesterol dependent cytolysin-group of toxins, the variant undecapeptide sequence EATGLAWDPWW which appeared to be most closely related to arcanolysin of Arcanobacterium haemolyticum and pyolysin of Trueperella pyogenes . In addition, gene phl could be determined with a newly designed loop-mediated isothermal amplification (LAMP) assay. The detection of mass spectra by MALDI-TOF MS and the LAMP assay based on gene phl might help to reliably identify A. phocae in future and also elucidate the role this species plays in infections of fur animals.
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Arcanobacterium wilhelmae sp nov isolated from the genital tract of a rhinoceros rhinoceros unicornis
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Osama Sammra, Mazen Alssahen, Jörn-peter Wickhorst, Christoph Lämmler, A. A. Hassan, Jorg Rau, Peter Kampfer, Stefanie P Glaeser, Hansjurgen BusseAbstract:A taxonomic study using a polyphasic approach was performed on an unidentified Arcanobacterium -like Gram-stain-positive bacterium isolated from the genital tract of a rhinoceros. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium canis (98.8 % 16S rRNA gene sequence similarity), Arcanobacterium phocisimile (97.8 %), Arcanobacterium phocae (97.7 %), Arcanobacterium haemolyticum (97.4 %), Arcanobacterium hippocoleae (96.6 %), Arcanobacterium pinnipediorum (96.4 %) and Arcarnobacterium pluranimalium (95.4 %). DNA–DNA hybridization values between strain 647T and Arcanobacterium canis DSM 25104T were very low, 13.4 % (reciprocal 15.9 %). The genomic DNA G+C content of strain 647T was 58.7 mol%. The presence of the major menaquinone MK-9(H4) supported the affiliation of this strain to the genus Arcanobacterium . The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine and an unidentified phosphoglycolipid. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium . Based on these tests, it is proposed that the unknown bacterium should be classified as a representative of a novel species of the genus Arcanobacterium named Arcanobacterium wilhelmae sp. nov. The type strain is 647T (=DSM 102162T=LMG 29418T).
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Application of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella pyogenes isolated from various origins
Molecular and cellular probes, 2016Co-Authors: Amir Abdulmawjood, Mazen Alssahen, Osama Sammra, Jörn-peter Wickhorst, Christoph Lämmler, Abdulwahed Ahmed Hassan, Ellen Prenger-berninghoff, O. Hashim, Guenter KleinAbstract:In the present study 28 Trueperella pyogenes strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene cpn60 encoding chaperonin. No cross reaction could be observed with control strains representing four species of genus Trueperella and seven species of closely related genus Arcanobacterium. The present cpn60 LAMP assay might allow a reliable and low cost identification of T. pyogenes also in laboratories with less specified equipment.
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Arcanobacterium pinnipediorum sp nov isolated from a harbour seal
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Osama Sammra, Christoph Lämmler, Samy Nagib, Peter Kampfer, Stefanie P Glaeser, Hansjurgen Busse, Anna Balbutskaya, H Ulbegimohyla, Jan Golke, Ellen PrengerberninghoffAbstract:A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like, Gram-stain-positive bacterium, strain 2710T, isolated from a harbour seal. Comparative 16S rRNA gene sequence analysis showed that this bacterial strain belonged to the genus Arcanobacterium and was related most closely to the type strains of Arcanobacterium phocae (98.4 % similarity) and Arcanobacterium phocisimile (97.5 %). 16S rRNA gene sequence similarities to the type strains of other Arcanobacterium species were between 95.3 and 96.9 %. DNA-DNA hybridization values between strain 2710T and A. phocae DSM 10002T and A. phocisimile LMG 27073T were 4.7 % (reciprocal 56 %) and 23 % (reciprocal 7.7 %), respectively. The presence of the major menaquinone MK-9(H4) and a polar lipid profile with the major compounds diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside supported the affiliation of strain 2710T to the genus Arcanobacterium. The major fatty acids were C16:0, C18:1ω9c, C18:0 and C18:2ω6,9c/anteiso-C18:0. The peptidoglycan structure was of cross-linkage type A5α (l-Lys-l-Lys-d-Glu). Physiological and biochemical tests clearly distinguished the isolate from other members of the genus Arcanobacterium. Based on these tests, it is proposed that this unknown bacterium should be classified as a novel species of the genus Arcanobacterium, with the name Arcanobacterium pinnipediorum sp. nov. The type strain is 2710T ( = DSM 28752T = LMG 28298T).
M. Hijazin - One of the best experts on this subject based on the ideXlab platform.
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Short Communication
2016Co-Authors: H. Ülbegi-mohyla, M. Hijazin, J. Alber, Christoph Lämmler, Ellen Prenger-berninghoff, Amir Abdulmawjood, R. Weiß, A. A. Hassan, Michael ZschöckAbstract:Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic propertie
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further studies on Arcanobacterium phocisimile a novel species of genus Arcanobacterium
Journal of veterinary medicine, 2014Co-Authors: Osama Sammra, M. Hijazin, J. Alber, Christoph Lämmler, Amir Abdulmawjood, Samy Nagib, Ellen Prengerberninghoff, Anna Balbutskaya, Markus Timke, Markus KostrzewaAbstract:Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.
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identification of Arcanobacterium pluranimalium by matrix assisted laser desorption ionization time of flight mass spectrometry and as novel target by sequencing pluranimaliumlysin encoding gene pla
Veterinary Microbiology, 2014Co-Authors: Anna Balbutskaya, Osama Sammra, M. Hijazin, J. Alber, Christoph Lämmler, Samy Nagib, M Erhard, Geoffrey Foster, P N Wragg, Amir AbdulmawjoodAbstract:In the present study 13 Arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and the pluranimaliumlysin encoding gene pla. The detection of mass spectra by MALDI-TOF MS and the novel genotypic approach using gene pla might help to identify A. pluranimalium in future and might elucidate the role this species plays in infections of animals.
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further characteristics of Arcanobacterium canis a novel species of genus Arcanobacterium
Veterinary Microbiology, 2013Co-Authors: Osama Sammra, M. Hijazin, Christoph Lämmler, Amir Abdulmawjood, Samy Nagib, Ellen Prengerberninghoff, Anna Balbutskaya, Markus Kostrzewa, Shuping Zhang, Markus TimkeAbstract:Comparable to previously conducted phenotypical and genotypical investigations characterizing Arcanobacterium canis, a newly described species with the type strain A. canis DSM 25104 isolated from an otitis externa of a dog, four additional A. canis strains isolated from infections of three dogs and one cat could reliably be identified by phenotypic properties, by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and by sequencing the genomic targets 16S rDNA, 16S-23S rDNA intergenic spacer region, 23S rDNA, and the genes rpoB and gap. All four A. canis investigated in the present study were isolated from the infected animals together with several other bacterial species indicating that the pathogenic importance of A. canis remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approaches might help to identify A. canis in future and might elucidate the role this species plays in infections of dogs and cats.
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Arcanobacterium phocisimile sp nov isolated from harbour seals
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: M. Hijazin, Osama Sammra, J. Alber, Christoph Lämmler, Samy Nagib, Peter Kampfer, Stefanie P Glaeser, Hansjurgen Busse, H Ulbegimohyla, Johannes KassmannhuberAbstract:A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium -like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum CIP 103370T (98.4 % 16S rRNA gene sequence similarity), A. canis P6775T (97.4 %), A. phocae DSM 10002T (97.4 %), A. pluranimalium M430/94/2T (95.7 %) and A. hippocoleae CCUG 44697T (95.5 %). The presence of the major menaquinone MK-9(H4) supported the affiliation of the isolates with the genus Arcanobacterium . The polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and two unidentified glycolipids. The major fatty acids were C16 : 0, C18 : 0, C18 : 1ω9c and summed feature 5 (comprising C18 : 2ω6,9c and/or anteiso-C18 : 0). Physiological and biochemical tests clearly distinguished the isolates from other members of the genus Arcanobacterium . Based on the common origin and various physiological properties comparable to those of A. phocae , it is proposed that the isolates are classified as members of a novel species with the name Arcanobacterium phocisimile sp. nov. The type strain is 2698T ( = LMG 27073T = CCM 8430T).
J. Alber - One of the best experts on this subject based on the ideXlab platform.
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Short Communication
2016Co-Authors: H. Ülbegi-mohyla, M. Hijazin, J. Alber, Christoph Lämmler, Ellen Prenger-berninghoff, Amir Abdulmawjood, R. Weiß, A. A. Hassan, Michael ZschöckAbstract:Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic propertie
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further studies on Arcanobacterium phocisimile a novel species of genus Arcanobacterium
Journal of veterinary medicine, 2014Co-Authors: Osama Sammra, M. Hijazin, J. Alber, Christoph Lämmler, Amir Abdulmawjood, Samy Nagib, Ellen Prengerberninghoff, Anna Balbutskaya, Markus Timke, Markus KostrzewaAbstract:Arcanobacterium phocisimile, a newly described species with the type strain A. phocisimile 2698(T) isolated from a vaginal swab of a harbour seal and four additional A. phocisimile strains also isolated from four harbour seals could reliably be identified by phenotypic properties, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and by sequencing the genomic targets 16S rDNA and 16S-23S rDNA intergenic spacer region and the genes rpoB and gap. The A. phocisimile strains investigated in the present study were isolated together with several other bacterial species indicating that the pathogenic importance of A. phocisimile remains unclear. However, the detection of peptidic spectra by MALDI-TOF MS and the presented phenotypic and genotypic approach might help to identify A. phocisimile in future.
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identification of Arcanobacterium pluranimalium by matrix assisted laser desorption ionization time of flight mass spectrometry and as novel target by sequencing pluranimaliumlysin encoding gene pla
Veterinary Microbiology, 2014Co-Authors: Anna Balbutskaya, Osama Sammra, M. Hijazin, J. Alber, Christoph Lämmler, Samy Nagib, M Erhard, Geoffrey Foster, P N Wragg, Amir AbdulmawjoodAbstract:In the present study 13 Arcanobacterium pluranimalium strains isolated from various animal origin could successfully be identified phenotypically by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and genotypically by sequencing 16S rDNA and the pluranimaliumlysin encoding gene pla. The detection of mass spectra by MALDI-TOF MS and the novel genotypic approach using gene pla might help to identify A. pluranimalium in future and might elucidate the role this species plays in infections of animals.
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Arcanobacterium phocisimile sp nov isolated from harbour seals
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: M. Hijazin, Osama Sammra, J. Alber, Christoph Lämmler, Samy Nagib, Peter Kampfer, Stefanie P Glaeser, Hansjurgen Busse, H Ulbegimohyla, Johannes KassmannhuberAbstract:A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium -like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related to Arcanobacterium haemolyticum CIP 103370T (98.4 % 16S rRNA gene sequence similarity), A. canis P6775T (97.4 %), A. phocae DSM 10002T (97.4 %), A. pluranimalium M430/94/2T (95.7 %) and A. hippocoleae CCUG 44697T (95.5 %). The presence of the major menaquinone MK-9(H4) supported the affiliation of the isolates with the genus Arcanobacterium . The polar lipid profile consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phospholipid and two unidentified glycolipids. The major fatty acids were C16 : 0, C18 : 0, C18 : 1ω9c and summed feature 5 (comprising C18 : 2ω6,9c and/or anteiso-C18 : 0). Physiological and biochemical tests clearly distinguished the isolates from other members of the genus Arcanobacterium . Based on the common origin and various physiological properties comparable to those of A. phocae , it is proposed that the isolates are classified as members of a novel species with the name Arcanobacterium phocisimile sp. nov. The type strain is 2698T ( = LMG 27073T = CCM 8430T).
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Arcanobacterium canis sp nov isolated from otitis externa of a dog and emended description of the genus Arcanobacterium collins et al 1983 emend yassin et al 2011
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: M. Hijazin, Osama Sammra, J. Alber, Christoph Lämmler, A. A. Hassan, Peter Kampfer, Stefanie P Glaeser, Hansjurgen Busse, Ellen Prengerberninghoff, Amir AbdulmawjoodAbstract:A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from otitis externa of a dog. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strains of Arcanobacterium haemolyticum (97.2 %), Arcanobacterium hippocoleae (96.5 %) and Arcanobacterium phocae (96.4 %). The presence of the major menaquinone MK-9(H(4)) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile contained the major lipids phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol mannoside and an unidentified phospholipid (PL2). Major fatty acids were C(14 : 0), C(16 : 0), C(18 : 0), C(18 : 1)ω9c and C(18 : 2)ω6,9c/anteiso-C(18 : 0) (detected as a summed feature). C(10 : 0) and C(12 : 0) were present in minor amounts. The results of physiological and biochemical testing clearly distinguished the unknown bacterium from other species of the genus Arcanobacterium. Based on these tests, it is proposed that the unknown bacterium should be classified in the novel species Arcanobacterium canis sp. nov. The type strain of Arcanobacterium canis is P6775(T) (= CCM 7958(T) = CCUG 61573(T) = CIP 110339(T)). An emended description of the genus Arcanobacterium is also provided.