The Experts below are selected from a list of 324 Experts worldwide ranked by ideXlab platform
Hironori Kaminaka - One of the best experts on this subject based on the ideXlab platform.
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The effects of gibberellin on the expression of symbiosis-related genes in Paris-type arbuscular mycorrhizal symbiosis in Eustoma grandiflorum.
Plant Signaling & Behavior, 2020Co-Authors: Takaya Tominaga, Masahide Yamato, Katsushi Yamaguchi, Shuji Shigenobu, Hironori KaminakaAbstract:Arbuscular mycorrhiza (AM) is a symbiotic interaction in terrestrial plants that is colonized by fungi in the Glomeromycotina. The morphological types of AM, including the Arum-type and Paris-type, are distinct, depending on the host plant species. A part of the regulatory pathways in Arum-type AM symbiosis has been revealed because most model plants form the Arum-type AM with a model AM fungus, Rhizophagus irregularis. Moreover, gibberellin (GA) is known to severely inhibit AM fungal colonization in Arum-type AM symbiosis. Recently, we showed that exogenous GA treatment significantly promoted AM fungal colonization in Paris-type AM symbiosis in Eustoma grandiflorum. In this study, we focused on the transcriptional changes in AM symbiosis-related genes in GA-treated E. grandiflorum. The expression levels of all examined E. grandiflorum genes were maintained or increased by GA treatment compared with those of the control treatment. Our new results suggest that signaling pathway(s) required for establishing AM symbiosis in E. grandiflorum may be distinct from the well-characterized pathway for that in model plants.
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Gibberellin Promotes Fungal Entry and Colonization during Paris-Type Arbuscular Mycorrhizal Symbiosis in Eustoma grandiflorum.
Plant & cell physiology, 2019Co-Authors: Takaya Tominaga, Chihiro Miura, Naoya Takeda, Yuri Kanno, Yoshihiro Takemura, Mitsunori Seo, Masahide Yamato, Hironori KaminakaAbstract:Arbuscular mycorrhizas (AMs) are divided into two types according to morphology: Arum- and Paris-type AMs. Gibberellins (GAs) mainly inhibit the establishment of Arum-type AM symbiosis in most model plants, whereas the effects of GAs on Paris-type AM symbiosis are unclear. To provide insight into the mechanism underlying this type of symbiosis, the roles of GAs were investigated in Eustoma grandiflorum when used as the host plant for Paris-type AM establishment. Eustoma grandiflorum seedlings were inoculated with the model AM fungus, Rhizophagus irregularis, and the effects of GA and the GA biosynthesis inhibitor uniconazole-P on the symbiosis were quantitatively evaluated. Exogenous GA significantly increased hyphopodium formation at the epidermis, thus leading to the promotion of fungal colonization and arbuscule formation in the root cortex. By contrast, the suppression of GA biosynthesis and signaling attenuated fungal entry to E. grandiflorum roots. Moreover, the exudates from GA-treated roots strongly induced the hyphal branching of R. irregularis. Our results show that GA has an contrasting effect on Paris-type AM symbiosis in E. grandiflorum compared with Arum-type AM symbiosis. This finding could be explained by the differential regulation of the early colonization stage, where fungal hyphae make contact with and penetrate the epidermis.
Di Zhou - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of 43 novel polymorphic est ssr markers for Arum lily zantedeschia aethiopica araceae
American Journal of Botany, 2012Co-Authors: Zunzheng Wei, Laibo Luo, Huali Zhang, Min Xiong, Xian Wang, Di ZhouAbstract:UNLABELLED PREMISE OF THE STUDY A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for Arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. • METHODS AND RESULTS Using 2175 unigenes derived from 4283 random ESTs in Arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. • CONCLUSIONS These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in Arum lily.
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Identification and characterization of 43 novel polymorphic EST‐SSR markers for Arum lily, Zantedeschia aethiopica (Araceae)
American journal of botany, 2012Co-Authors: Zunzheng Wei, Laibo Luo, Huali Zhang, Min Xiong, Xian Wang, Di ZhouAbstract:UNLABELLED PREMISE OF THE STUDY A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for Arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. • METHODS AND RESULTS Using 2175 unigenes derived from 4283 random ESTs in Arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. • CONCLUSIONS These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in Arum lily.
A. Lendel - One of the best experts on this subject based on the ideXlab platform.
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Comparative chromosome and molecular studies of some species of genus Arum from eastern Slavonia and Baranya region in Croatia
Acta Societatis Botanicorum Poloniae, 2011Co-Authors: M. Sabo, T. Bačić, A. Lendel, Marija Bedalov, Ljiljana Kristin, Tihana MarčekAbstract:Karyological and molecular studies were done in this paper on three species of genus Arum; Arum italicum Mill. and Arum maculatum L., with two varieties, and Arum alpinum Schott and Kotschy, also with two varieties. The main goal of this paper was to establish whether they were regularly determined exclusively on the principle of morphological parameters. Karyological studies showed that the number of chromosomes for Arum italicum Mill. amounted to 2n=84, for Arum maculatum L. 2n=56 and for Arum alpinum Schott and Kotschy 2n=28. This confirmed that these species are not only clean and separated, but also support the regularity of the morphological determination. Molecular studies, e.g. RAPD method showed that two genetically separated species groups correspond to the three mentioned species. Arum italicum Mill. is the least homogenous species closely related and the variability between populations is high. Arum maculatum L. is more homogenous within the species, two varieties could be differentiated whereas they are closely genetically related and the variability between the populations is too high. Arum alpinum is strongly homogenous and within these species two varieties could also be differentiated. That means that they are closely related and the variability between the populations is very high. These observations mostly coincided with previous morphological investigations.
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Comparative morphology of some species of genus Arum from Eastern Slavonia and Baranya region in Croatia
Acta Botanica Hungarica, 2007Co-Authors: A. Lendel, M. Sabo, T. Bačić, Ljiljana Krstin, Marija Bedalov, Tihana MarčekAbstract:Comparative variability in some morphological properties, for example, in leaves and flowers, among and within each of three species Arum italicum Mill., Arum maculatum L. var. maculatum and var. immaculatum Reichb. as well as Arum alpinum Schott et Kotschy var. pannonicum Terpo and var. intermedium Terpo from three localities in Eastern Slavonia and from two in Baranya region in Croatia were studied. The investigation included the variability among and within of the populations and habitats and among the species. The results obtained indicated significant differences chiefly in some morphological parameters, such as in length of petiole and peduncle, in length of spathe, in length and diameter of spathe-tube, in length and diameter of appendix as well as in length of pistillate and staminate flowers. Moreover, the taxonomic treatment of the species Arum alpinum Schott et Kotschy as a disagreement was also discussed. The most of the morphological features are in the concordance with observations made by P...
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Stomata in some species of genus Arum from the Eastern Slavonia and Baranya region
Biologia, 2007Co-Authors: M. Sabo, T. Bačić, Tihana Marček, Ljiljana Krstin, A. LendelAbstract:Types and the number of stomata in the following Arum species: Arum italicum Mill., Arum maculatum var. maculatum L. and Arum maculatum var. immaculatum L., Arum alpinum var. pannonicum Terpo. and Arum alpinum var. intermedium Schur. in three different locations in Zablace, Normanci and Bilje were investigated. The most prevalent stomata type at both upper and lower epidermis for each Arum species was mostly stomata type paracytic, followed by hexacytic, tetracytic or brachyparacytic as far as locality is concerned. Helicocytic type was more prevalent in Arum alpinum var. pannonicum Terpo. and Arum alpinum var. intermedium Schur. at Bilje. Other stomata types were very rare. A striking regularity in the occurrence of stomata types was not found within a single species. Some stomata types, however, were found either at adaxial or abaxial epidermis or were not present at all. The number of stomata per square mm varied from 25 to 651. A statistically significant difference in the number of stomata per square mm at upper and lower epidermis among Arum species was determined in locations Zablace and Normanci, whereas no statistically significant differences were found in location Bilje.
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Length and width of guard cells and variation in the appearance of stomata pores in some species of genus Arum from the Eastern Slavonia and Baranya Region
Acta Botanica Hungarica, 2004Co-Authors: M. Sabo, T. Lajdes, T. Bačić, L. Grgić, A. LendelAbstract:Length and width of guard cells and variation in the appearance of stomata pores in the following Arum species: Arum italicum Mill., Arum maculatum var. maculatum L. and Arum maculatum var. immaculatum L. at Zablace and Normanci location, and Arum alpinum var. pannonicum Terpo., Arum alpinum var. intermedium Schur. in Bilje at the eastern Slavonia and Baranya region were investigated. With regard to guard cells length and width and variation in the appearance of stomata pores, stomata of certain Arum species are considered to be of larger dimensions (≯38 µm). Arum species grown at Zablace had the longest and widest guard cells as well as the greatest variation in the appearance of stomata pores, followed by those at Normanci, whereas species at Bilje location had the lowest values. The average length and width of the guard cells and variation in the appearance of stomata pores were larger at the lower than at the upper epidermis among each examined Arum species at each location. A significant difference i...
Takaya Tominaga - One of the best experts on this subject based on the ideXlab platform.
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The effects of gibberellin on the expression of symbiosis-related genes in Paris-type arbuscular mycorrhizal symbiosis in Eustoma grandiflorum.
Plant Signaling & Behavior, 2020Co-Authors: Takaya Tominaga, Masahide Yamato, Katsushi Yamaguchi, Shuji Shigenobu, Hironori KaminakaAbstract:Arbuscular mycorrhiza (AM) is a symbiotic interaction in terrestrial plants that is colonized by fungi in the Glomeromycotina. The morphological types of AM, including the Arum-type and Paris-type, are distinct, depending on the host plant species. A part of the regulatory pathways in Arum-type AM symbiosis has been revealed because most model plants form the Arum-type AM with a model AM fungus, Rhizophagus irregularis. Moreover, gibberellin (GA) is known to severely inhibit AM fungal colonization in Arum-type AM symbiosis. Recently, we showed that exogenous GA treatment significantly promoted AM fungal colonization in Paris-type AM symbiosis in Eustoma grandiflorum. In this study, we focused on the transcriptional changes in AM symbiosis-related genes in GA-treated E. grandiflorum. The expression levels of all examined E. grandiflorum genes were maintained or increased by GA treatment compared with those of the control treatment. Our new results suggest that signaling pathway(s) required for establishing AM symbiosis in E. grandiflorum may be distinct from the well-characterized pathway for that in model plants.
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Gibberellin Promotes Fungal Entry and Colonization during Paris-Type Arbuscular Mycorrhizal Symbiosis in Eustoma grandiflorum.
Plant & cell physiology, 2019Co-Authors: Takaya Tominaga, Chihiro Miura, Naoya Takeda, Yuri Kanno, Yoshihiro Takemura, Mitsunori Seo, Masahide Yamato, Hironori KaminakaAbstract:Arbuscular mycorrhizas (AMs) are divided into two types according to morphology: Arum- and Paris-type AMs. Gibberellins (GAs) mainly inhibit the establishment of Arum-type AM symbiosis in most model plants, whereas the effects of GAs on Paris-type AM symbiosis are unclear. To provide insight into the mechanism underlying this type of symbiosis, the roles of GAs were investigated in Eustoma grandiflorum when used as the host plant for Paris-type AM establishment. Eustoma grandiflorum seedlings were inoculated with the model AM fungus, Rhizophagus irregularis, and the effects of GA and the GA biosynthesis inhibitor uniconazole-P on the symbiosis were quantitatively evaluated. Exogenous GA significantly increased hyphopodium formation at the epidermis, thus leading to the promotion of fungal colonization and arbuscule formation in the root cortex. By contrast, the suppression of GA biosynthesis and signaling attenuated fungal entry to E. grandiflorum roots. Moreover, the exudates from GA-treated roots strongly induced the hyphal branching of R. irregularis. Our results show that GA has an contrasting effect on Paris-type AM symbiosis in E. grandiflorum compared with Arum-type AM symbiosis. This finding could be explained by the differential regulation of the early colonization stage, where fungal hyphae make contact with and penetrate the epidermis.
Zunzheng Wei - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of 43 novel polymorphic est ssr markers for Arum lily zantedeschia aethiopica araceae
American Journal of Botany, 2012Co-Authors: Zunzheng Wei, Laibo Luo, Huali Zhang, Min Xiong, Xian Wang, Di ZhouAbstract:UNLABELLED PREMISE OF THE STUDY A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for Arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. • METHODS AND RESULTS Using 2175 unigenes derived from 4283 random ESTs in Arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. • CONCLUSIONS These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in Arum lily.
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Identification and characterization of 43 novel polymorphic EST‐SSR markers for Arum lily, Zantedeschia aethiopica (Araceae)
American journal of botany, 2012Co-Authors: Zunzheng Wei, Laibo Luo, Huali Zhang, Min Xiong, Xian Wang, Di ZhouAbstract:UNLABELLED PREMISE OF THE STUDY A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for Arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. • METHODS AND RESULTS Using 2175 unigenes derived from 4283 random ESTs in Arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. • CONCLUSIONS These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in Arum lily.
