The Experts below are selected from a list of 30249 Experts worldwide ranked by ideXlab platform
Paul Bastard - One of the best experts on this subject based on the ideXlab platform.
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auto antibodies to type i ifns can underlie adverse reactions to yellow fever live Attenuated Vaccine
Journal of Experimental Medicine, 2021Co-Authors: Paul Bastard, Eleftherios Michailidis, Hansheinrich Hoffmann, Marwa Chbihi, Tom Le Voyer, Jeremie RosainAbstract:Yellow fever virus (YFV) live Attenuated Vaccine can, in rare cases, cause life-threatening disease, typically in patients with no previous history of severe viral illness. Autosomal recessive (AR) complete IFNAR1 deficiency was reported in one 12-yr-old patient. Here, we studied seven other previously healthy patients aged 13 to 80 yr with unexplained life-threatening YFV Vaccine-associated disease. One 13-yr-old patient had AR complete IFNAR2 deficiency. Three other patients vaccinated at the ages of 47, 57, and 64 yr had high titers of circulating auto-Abs against at least 14 of the 17 individual type I IFNs. These antibodies were recently shown to underlie at least 10% of cases of life-threatening COVID-19 pneumonia. The auto-Abs were neutralizing in vitro, blocking the protective effect of IFN-α2 against YFV Vaccine strains. AR IFNAR1 or IFNAR2 deficiency and neutralizing auto-Abs against type I IFNs thus accounted for more than half the cases of life-threatening YFV Vaccine-associated disease studied here. Previously healthy subjects could be tested for both predispositions before anti-YFV vaccination.
Peng Zhao - One of the best experts on this subject based on the ideXlab platform.
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Chicken infectious anemia virus helps fowl adenovirus break the protection of maternal antibody and cause inclusion body hepatitis-hydropericardium syndrome in layers after using co-contaminated Newcastle disease virus-Attenuated Vaccine
Poultry science, 2019Co-Authors: Fanfeng Meng, Zhihui Zhang, Zhizhong Cui, Shuang Chang, Yankun Zhang, Peng ZhaoAbstract:Inclusion body hepatitis-hydropericardium syndrome (IBH-HPS) caused by fowl adenovirus type 4 (FAdV-4) has caused huge economic losses for China in the past five years. At present, this disease is controlled in many flocks with the inactivated FAdV Vaccine, but the offspring chicks of a layer breeding flock that were vaccinated with this Vaccine still became infected and developed IBH-HPS with a 20% mortality rate. Analysis revealed that the NDV-Attenuated Vaccine in use from the above-mentioned poultry farm was simultaneously contaminated with FAdV-4 and chicken infectious anemia virus (CIAV). The FAdV and CIAV isolated from the Vaccine were purified for the artificial preparation of an NDV-Attenuated Vaccine singly contaminated with FAdV or CIAV, or simultaneously contaminated with both of them. Seven-day-old layers with maternal FAdV antibody were inoculated with the artificially prepared, contaminated Vaccines and assessed for corresponding indices. The experiments showed that no obvious symptoms occurred after using the NDV-Attenuated Vaccine singly contaminated with FAdV or CIAV; however, common IBH and occasional HPS-related death was found in birds after administering the NDV-Attenuated Vaccine co-contaminated with FAdV and CIAV. In conclusion, this study illustrated that CIAV could assist FAdV in breaking maternal FAdV antibody protection, which then caused the IBH-HPS after vaccination with the co-contaminated NDV Vaccine.
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The intracorporal interaction of fowl adenovirus type 4 and LaSota strain significantly aggravates the pathogenicity of one another after using contaminated Newcastle disease virus-Attenuated Vaccine.
Poultry science, 2019Co-Authors: Xiaofeng Liu, Fanfeng Meng, Zhizhong Cui, Shuang Chang, Peng ZhaoAbstract:In the past five years, inclusion body hepatitis-hydropericardium syndrome caused by fowl adenovirus type 4 (FAdV-4) has been rampant in China and is still an epidemic at present. We had found the contamination of FAdV-4 in Newcastle disease virus (NDV)-Attenuated Vaccine from a large farm with aforementioned disease, and then conjectured that the use of this contaminated Vaccine might be an important reason for the outbreak of the FAdV-4 infection. To assess the pathogenicity of this contaminated Vaccine and the interaction between FAdV and LaSota, systemic animal experiments were launched with FAdV infection via the contaminated Vaccine, as well as FAdV direct infection. Results showed that no chicks died after FAdV direct infection, while a 16.7% mortality rate appeared after FAdV infection through the contaminated Vaccine; the synergistic reaction of FAdV and LaSota aggravated their inhibitory action for weight growth of SPF chickens, enhanced one another's capacity for immune system destruction, promoted their multiplication in vivo, and restrained antibody production. This study demonstrated the intracorporal interaction and enhanced pathogenicity of FAdV-4 and LaSota strain in contaminated NDV-Attenuated Vaccine, illustrating how the exogenous virus causes infection and induces severe diseases, which reminds us that the damage of Attenuated Vaccine contaminated with FAdV-4 even in extremely low dose is not insignificant.
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Newcastle disease virus-Attenuated Vaccine LaSota played a key role in the pathogenicity of contaminated exogenous virus
Veterinary research, 2018Co-Authors: Yawen Zhang, Zhihui Zhang, Fanfeng Meng, Zhizhong Cui, Shuang Chang, Peng ZhaoAbstract:Newcastle disease virus (NDV)-Attenuated Vaccine has been widely used since the 1950s and made great progress in preventing and controlling Newcastle disease. However, many reports mention exogenous virus contamination in Attenuated Vaccines, while co-contamination with fowl adenovirus (FAdV) and chicken infectious anaemia virus (CIAV) in the NDV-Attenuated Vaccine also emerged in China recently, which proved to be an important reason for the outbreaks of inclusion body hepatitis–hydropericardium syndrome in some flocks. It is amazing that exogenous virus contamination at extremely low doses still infected chickens and induced severe disease; thus, we speculated that there must be some interaction between the NDV-Attenuated Vaccine and the contaminated exogenous viruses within. Accordingly, simulation experiments were launched using FAdV and CIAV isolated from the abovementioned Vaccine. The results showed that the pathogenicity of FAdV and CIAV co-infection through the contaminated Vaccine was significantly higher than that of direct oral infection, while the synergistic reaction of these viruses and LaSota prompted their multiplication in vivo and disturbed the production of antibodies against each other. This study showed the interactions of FAdV, CIAV and LaSota after using contaminated NDV-Attenuated Vaccine, helping us to understand how the contaminated exogenous viruses cause infection and induce severe disease at a relatively low dose through the oral route.
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Newcastle disease virus-Attenuated Vaccine co-contaminated with fowl adenovirus and chicken infectious anemia virus results in inclusion body hepatitis-hydropericardium syndrome in poultry.
Veterinary microbiology, 2018Co-Authors: Fanfeng Meng, Zhizhong Cui, Shuang Chang, Peng ZhaoAbstract:Inclusion body hepatitis-hydropericardium syndrome (IBH-HPS) induced by fowl adenovirus type 4 (FAdV-4) has caused huge economic losses to the poultry industry of China, but the source of infection for different flocks, especially flocks with high biological safety conditions, has remained unclear. This study tested the pathogenicity of Newcastle disease virus (NDV)-Attenuated Vaccine from a large-scale poultry farm in China where IBH-HPS had appeared with high mortality. Analysis revealed that the NDV-Attenuated Vaccine in use from the abovementioned poultry farm was simultaneously contaminated with FAdV-4 and chicken infectious anemia virus (CIAV). The FAdV and CIAV isolated from the Vaccine were purified for the artificial preparation of an NDV-Attenuated Vaccine singly contaminated with FAdV or CIAV, or simultaneously contaminated with both of them. Seven-day-old specific pathogen-free chicks were inoculated with the artificially prepared contaminated Vaccines and tested for corresponding indices. The experiments showed that no hydropericardium syndrome (HPS) and corresponding death occurred after administering the NDV-Attenuated Vaccine singly contaminated with FAdV or CIAV, but a mortality of 75% with IBH-HPS was commonly found in birds after administering the NDV-Attenuated Vaccine co-contaminated with FAdV and CIAV. In conclusion, this study found the co-contamination of FAdV-4 and CIAV in the same Attenuated Vaccine and confirmed that such a contaminated Attenuated Vaccine was a significant source of infection for outbreaks of IBH-HPS in some flocks.
Yuanxing Zhang - One of the best experts on this subject based on the ideXlab platform.
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An invasive and low virulent Edwardsiella tarda esrB mutant promising as live Attenuated Vaccine in aquaculture
Applied microbiology and biotechnology, 2014Co-Authors: Weizheng Yang, Qiyao Wang, Lixia Wang, Lingzhi Zhang, Yuanxing ZhangAbstract:Edwardsiella tarda is a leading fish pathogen haunting worldwide aquaculture industry. In E. tarda, two-component system EsrA-EsrB positively regulates type III and VI secretion systems (T3SS and T6SS) and negatively regulates hemolysin EthA, which has been demonstrated to be essential for the invasion processes in fish. In order to develop a live Attenuated Vaccine (LAV) with high invasiveness to be practically and economically used as immersion-administered Vaccine in aquaculture, here, we generated a random mutation library of esrB sequences by error-prone PCR and introduced them into the E. tarda esrB deletion mutant. The mutant YWZ47 with significantly increased hemolytic activity and low T3SS and T6SS secretion was screened. Phenotypes including extracellular protein profiles, invasion in macrophages, lethality toward fish, and infection kinetics were investigated in the wild-type strain EIB202 and the mutants ΔesrB, ΔT3SS, ΔT6SS, ΔT3SS/ΔT6SS, and YWZ47. Compared to the documented LAV strain ΔesrB, YWZ47 showed higher invasive capability and low in vivo virulence toward fish. Significantly higher relative percent survival (RPS) could be generated in turbot (Scophthalmus maximus) against the challenge of the wild-type EIB202 when inoculated through immersion route, and the RPS was comparable with that of ΔesrB through intraperitoneal (i.p.) injection inoculation. Two mutated points, K167M and H197L, were found by sequence analysis of EsrBYWZ47 variant. These structural modifications underpin the variations in the regulatory functions of the mutant and wild-type EsrB. This study promoted understanding of virulence regulation by EsrB in E. tarda and presented a promising candidate of invasive Attenuated Vaccine used in aquaculture industries.
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edwardsiella tarda mutant disrupted in type iii secretion system and chorismic acid synthesis and cured of a plasmid as a live Attenuated Vaccine in turbot
Fish & Shellfish Immunology, 2013Co-Authors: Jingfan Xiao, Weizheng Yang, Qiyao Wang, Jiangbo Qu, Tao Chen, Yuanxing ZhangAbstract:Edwardsiella tarda is an intractable Gram-negative pathogen in many fish species to cause edwardsiellosis. Its infection leads to extensive losses in a diverse array of commercially important fish. The type III secretion system (T3SS) has been considered as one of the major virulence factors and plays important roles in its intracellular lifestyle. In this study, an E. tarda EIB202 mutant WED with deletions in the T3SS genes for EseB, EseC, EseD and EscA, along with the aroC gene for the biosynthesis of chorismic acid, as well as the curing of endogenous plasmid pEIB202 was constructed by allelic exchange strategy. Compared to the wild-type EIB202 which was highly virulent towards turbot (Scophthamus maximus) via intraperitoneal (i.p.), intramuscular (i.m.) injection or immersion and caused systemic infection in turbot as well as the unexpected red mouth symptom when immersion challenged, WED was highly Attenuated when inoculated into turbot via i.m., i.p. and immersion routes, and exhibited significantly impaired capacity to survive in fish tissues. WED showed 5700-fold higher 50% lethal dose (LD50) than that of the wild type when i.m. or i.p. challenged. Inoculation with WED by i.p. or immersion injection routes elicited significant protection against the challenge of the wild-type E. tarda after 5 weeks of vaccination. The vaccinated fish produced low while significant level of specific antibody and showed increased expression of immune-related factors including IL-1β, IFN-γ, MHC II, MHC-I and CD8, indicating that WED possesses significant immunoprotective potential. Furthermore, our data indicated that a single dose of i.p. and immersion vaccination with WED could produce significant protection as long as 12 and 6 months, respectively. These results demonstrated the feasibility of WED as a live Attenuated Vaccine in turbot against edwardsiellosis by immersion or i.p. injection routes.
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Search for live Attenuated Vaccine candidate against edwardsiellosis by mutating virulence‐related genes of fish pathogen Edwardsiella tarda
Letters in applied microbiology, 2011Co-Authors: Jingfan Xiao, Qiyao Wang, Tao Chen, Q. Liu, Xuedong Wang, Yuanxing ZhangAbstract:Aims: The aims of this study were to construct and evaluate the live Attenuated Vaccine against edwardsiellosis on zebra fish model. Methods and Results: In this study, the deletion mutant of aroC gene for the biosynthesis of chorismic acid in Edwardsiella tarda EIB202 was firstly constructed by allelic exchange strategy. According to the genome information, 19 double mutants and one multiple mutant were successively constructed by deleting virulence-associated genes based on the ΔaroC mutant. Zebra fish model was used to assay the virulence of the mutants by intramuscular (i.m.) injection. Fourteen mutants were significantly Attenuated with accumulated mortality ranged from 0 to 63% (P
Alan D.t. Barrett - One of the best experts on this subject based on the ideXlab platform.
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Yellow fever live Attenuated Vaccine: A very successful live Attenuated Vaccine but still we have problems controlling the disease
Vaccine, 2017Co-Authors: Alan D.t. BarrettAbstract:Yellow fever (YF) is regarded as the original hemorrhagic fever and has been a major public health problem for at least 250years. A very effective live Attenuated Vaccine, strain 17D, was developed in the 1930s and this has proved critical in the control of the disease. There is little doubt that without the Vaccine, YF virus would be considered a biosafety level 4 pathogen. Significantly, YF is currently the only disease where an international vaccination certificate is required under the International Health Regulations. Despite having a very successful Vaccine, there are occasional issues of supply and demand, such as that which occurred in Angola and Democratic Republic of Congo in 2016 when there was insufficient Vaccine available. For the first time fractional dosing of the Vaccine was approved on an emergency basis. Thus, continued vigilance and improvements in supply and demand are needed in the future.
Melkote S. Shaila - One of the best experts on this subject based on the ideXlab platform.
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Immunogenic and protective properties of haemagglutinin protein (H) of rinderpest virus expressed by a recombinant baculovirus.
Vaccine, 1997Co-Authors: Sangeeta Naik, Gourapura J. Renukaradhya, M. Rajasekhar, Melkote S. ShailaAbstract:The hemagglutinin (H) protein of Rinderpest virus expressed by a recombinant baculo-virus used as a Vaccine produced high titres of neutralizing antibody to Rinderpest virus in the vaccinated cattle, comparable to the levels produced by live Attenuated Vaccine. The immunized cattle were protected against a Vaccine-virus challenge, as demonstrated by the failure of development of antibodies to N protein of the Vaccine virus. The lack of replication of Vaccine virus in the immunized cattle indicated that they are capable of showing a protective response if challenged with a virulent virus.
