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Kalaivani Thiagarajan - One of the best experts on this subject based on the ideXlab platform.

  • Neuroprotective effect of Tagara, an Ayurvedic Drug against methyl mercury induced oxidative stress using rat brain mitochondrial fractions
    BMC Complementary and Alternative Medicine, 2015
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Background Methyl mercury (MeHg), an important environmental toxicant is implicated in neurological disorders such as Hunter-Russell syndrome and Autism. Therefore, the present work is in search of new Drugs that can alleviate MeHg toxicity. In this connection, Tagara, an Ayurvedic Drug is used for assessing its neuro protective effect against MeHg toxicity. Methods In the present study, we assessed the phytochemical contents of Tagara by colorimetric and HPLC analyses. The neuroprotective effect of Tagara on MeHg induced neurotoxicity was measured in terms of viability by MTT assay and oxidative stress in terms of catalase activity, glutathione and thiobarbituric acid reactive substance levels. Further, the chelating effect of Tagara towards MeHg was performed to identify the molecular mechanism. Statistical analysis was done by statistical package for social sciences (SPSS) version 16.0. Results The results demonstrated that Tagara contains significant amounts of phenols and flavonoids. Also, HPLC analysis of Tagara revealed the presence of essential oils such as hydroxyvalerenic and valerenic acids. Our results demonstrated that exposure of rat brain mitochondrial fractions to MeHg resulted in a dose dependent death in MTT assay and IC_50 value was found to be 10 μM. However, a 250 μg dose of Tagara effectively prevented MeHg induced mitochondrial damage. The oxidative stress caused by MeHg results in elevated levels of reactive oxygen species as evidenced by elevated TBARS (Thiobarbituric acid-reactive substances) levels and diminished catalase enzyme activity and glutathione content. However, Tagara at 250 μg concentration offsets these alterations caused by MeHg. Further, Tagara also diminished GSH oxidation caused by MeHg, confirming its chelating effect, one of the molecular mechanisms that triggers protection against oxidative damage. Conclusion Our results revealed that MeHg induced toxicity is predominantly mediated through oxidative stress mechanism and the propensity of Tagara to abolish such reactions. Hence, we propose that Tagara with a source of potential neuroprotectants may be a useful approach to alleviate MeHg associated neurotoxicity.

  • Neuroprotective effect of Tagara, an Ayurvedic Drug against methyl mercury induced oxidative stress using rat brain mitochondrial fractions
    BMC complementary and alternative medicine, 2015
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Methyl mercury (MeHg), an important environmental toxicant is implicated in neurological disorders such as Hunter-Russell syndrome and Autism. Therefore, the present work is in search of new Drugs that can alleviate MeHg toxicity. In this connection, Tagara, an Ayurvedic Drug is used for assessing its neuro protective effect against MeHg toxicity. In the present study, we assessed the phytochemical contents of Tagara by colorimetric and HPLC analyses. The neuroprotective effect of Tagara on MeHg induced neurotoxicity was measured in terms of viability by MTT assay and oxidative stress in terms of catalase activity, glutathione and thiobarbituric acid reactive substance levels. Further, the chelating effect of Tagara towards MeHg was performed to identify the molecular mechanism. Statistical analysis was done by statistical package for social sciences (SPSS) version 16.0. The results demonstrated that Tagara contains significant amounts of phenols and flavonoids. Also, HPLC analysis of Tagara revealed the presence of essential oils such as hydroxyvalerenic and valerenic acids. Our results demonstrated that exposure of rat brain mitochondrial fractions to MeHg resulted in a dose dependent death in MTT assay and IC50 value was found to be 10 μM. However, a 250 μg dose of Tagara effectively prevented MeHg induced mitochondrial damage. The oxidative stress caused by MeHg results in elevated levels of reactive oxygen species as evidenced by elevated TBARS (Thiobarbituric acid-reactive substances) levels and diminished catalase enzyme activity and glutathione content. However, Tagara at 250 μg concentration offsets these alterations caused by MeHg. Further, Tagara also diminished GSH oxidation caused by MeHg, confirming its chelating effect, one of the molecular mechanisms that triggers protection against oxidative damage. Our results revealed that MeHg induced toxicity is predominantly mediated through oxidative stress mechanism and the propensity of Tagara to abolish such reactions. Hence, we propose that Tagara with a source of potential neuroprotectants may be a useful approach to alleviate MeHg associated neurotoxicity.

  • Natural Product Research: Formerly Natural Product Letters
    2014
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran & KalaivaniThiagarajan (2014): Neuroprotective effect of Brahmi, an Ayurvedic Drug against oxidative stressinduced by methyl mercury toxicity in rat brain mitochondrial-enriched fractions, Natural ProductResearch: Formerly Natural Product Letters, DOI: 10.1080/14786419.2014.968153

Dhanoop Manikoth Ayyathan - One of the best experts on this subject based on the ideXlab platform.

  • Neuroprotective effect of Tagara, an Ayurvedic Drug against methyl mercury induced oxidative stress using rat brain mitochondrial fractions
    BMC Complementary and Alternative Medicine, 2015
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Background Methyl mercury (MeHg), an important environmental toxicant is implicated in neurological disorders such as Hunter-Russell syndrome and Autism. Therefore, the present work is in search of new Drugs that can alleviate MeHg toxicity. In this connection, Tagara, an Ayurvedic Drug is used for assessing its neuro protective effect against MeHg toxicity. Methods In the present study, we assessed the phytochemical contents of Tagara by colorimetric and HPLC analyses. The neuroprotective effect of Tagara on MeHg induced neurotoxicity was measured in terms of viability by MTT assay and oxidative stress in terms of catalase activity, glutathione and thiobarbituric acid reactive substance levels. Further, the chelating effect of Tagara towards MeHg was performed to identify the molecular mechanism. Statistical analysis was done by statistical package for social sciences (SPSS) version 16.0. Results The results demonstrated that Tagara contains significant amounts of phenols and flavonoids. Also, HPLC analysis of Tagara revealed the presence of essential oils such as hydroxyvalerenic and valerenic acids. Our results demonstrated that exposure of rat brain mitochondrial fractions to MeHg resulted in a dose dependent death in MTT assay and IC_50 value was found to be 10 μM. However, a 250 μg dose of Tagara effectively prevented MeHg induced mitochondrial damage. The oxidative stress caused by MeHg results in elevated levels of reactive oxygen species as evidenced by elevated TBARS (Thiobarbituric acid-reactive substances) levels and diminished catalase enzyme activity and glutathione content. However, Tagara at 250 μg concentration offsets these alterations caused by MeHg. Further, Tagara also diminished GSH oxidation caused by MeHg, confirming its chelating effect, one of the molecular mechanisms that triggers protection against oxidative damage. Conclusion Our results revealed that MeHg induced toxicity is predominantly mediated through oxidative stress mechanism and the propensity of Tagara to abolish such reactions. Hence, we propose that Tagara with a source of potential neuroprotectants may be a useful approach to alleviate MeHg associated neurotoxicity.

  • Neuroprotective effect of Tagara, an Ayurvedic Drug against methyl mercury induced oxidative stress using rat brain mitochondrial fractions
    BMC complementary and alternative medicine, 2015
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Methyl mercury (MeHg), an important environmental toxicant is implicated in neurological disorders such as Hunter-Russell syndrome and Autism. Therefore, the present work is in search of new Drugs that can alleviate MeHg toxicity. In this connection, Tagara, an Ayurvedic Drug is used for assessing its neuro protective effect against MeHg toxicity. In the present study, we assessed the phytochemical contents of Tagara by colorimetric and HPLC analyses. The neuroprotective effect of Tagara on MeHg induced neurotoxicity was measured in terms of viability by MTT assay and oxidative stress in terms of catalase activity, glutathione and thiobarbituric acid reactive substance levels. Further, the chelating effect of Tagara towards MeHg was performed to identify the molecular mechanism. Statistical analysis was done by statistical package for social sciences (SPSS) version 16.0. The results demonstrated that Tagara contains significant amounts of phenols and flavonoids. Also, HPLC analysis of Tagara revealed the presence of essential oils such as hydroxyvalerenic and valerenic acids. Our results demonstrated that exposure of rat brain mitochondrial fractions to MeHg resulted in a dose dependent death in MTT assay and IC50 value was found to be 10 μM. However, a 250 μg dose of Tagara effectively prevented MeHg induced mitochondrial damage. The oxidative stress caused by MeHg results in elevated levels of reactive oxygen species as evidenced by elevated TBARS (Thiobarbituric acid-reactive substances) levels and diminished catalase enzyme activity and glutathione content. However, Tagara at 250 μg concentration offsets these alterations caused by MeHg. Further, Tagara also diminished GSH oxidation caused by MeHg, confirming its chelating effect, one of the molecular mechanisms that triggers protection against oxidative damage. Our results revealed that MeHg induced toxicity is predominantly mediated through oxidative stress mechanism and the propensity of Tagara to abolish such reactions. Hence, we propose that Tagara with a source of potential neuroprotectants may be a useful approach to alleviate MeHg associated neurotoxicity.

  • Natural Product Research: Formerly Natural Product Letters
    2014
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran & KalaivaniThiagarajan (2014): Neuroprotective effect of Brahmi, an Ayurvedic Drug against oxidative stressinduced by methyl mercury toxicity in rat brain mitochondrial-enriched fractions, Natural ProductResearch: Formerly Natural Product Letters, DOI: 10.1080/14786419.2014.968153

Rajesh Dabur - One of the best experts on this subject based on the ideXlab platform.

  • Intervention of Ayurvedic Drug Tinospora cordifolia attenuates the metabolic alterations in hypertriglyceridemia: a pilot clinical trial
    Journal of Diabetes & Metabolic Disorders, 2020
    Co-Authors: Amey Shirolkar, Aarti Yadav, T. K. Mandal, Rajesh Dabur
    Abstract:

    Purpose Hypertriglyceridemia (HG) is an independent risk factor with more prevalence than hypercholesterolemia and its attributes to cardiovascular disease (CVD) and pancreatitis. Hence, it becomes imperative to search for new triglyceride (TG) lowering agents. Tinospora cordifolia (TC) is a well-known Ayurvedic Drug and a rich source of protoberberine alkaloids hence can contribute to TG lowering without side effects. Hence, to explore the therapeutic efficacy of T. cordifolia and its effects on biochemistry and metabolome in the patients of hyper-triglyceridemia, clinical trials were conducted. Methods Patients (n = 24) with hypertriglyceridemia were randomized into two groups to receive T. cordifolia extract (TCE) (3.0 g/per day) and metformin (850 mg/day) for 14 days having >300 mg/dl triglyceride level and cholesterol in the range of 130–230 mg/dl. Lipid profiles of blood samples were analyzed. Urine samples were subjected to HPLC-QTOF-MS to quantify oxidative damage and abnormal metabolic regulation. Results Intervention with TCE reduced the triglyceride, LDL, and VLDL levels to 380.45 ± 17.44, 133.25 ± 3.18, and 31.85 ± 5.88 mg/dL and increased the HDL to 47.50 ± 9.05 mg/dL significantly (p 

  • intervention of Ayurvedic Drug tinospora cordifolia attenuates the metabolic alterations in hypertriglyceridemia a pilot clinical trial
    Journal of diabetes and metabolic disorders, 2020
    Co-Authors: Amey Shirolkar, Aarti Yadav, T. K. Mandal, Rajesh Dabur
    Abstract:

    Purpose Hypertriglyceridemia (HG) is an independent risk factor with more prevalence than hypercholesterolemia and its attributes to cardiovascular disease (CVD) and pancreatitis. Hence, it becomes imperative to search for new triglyceride (TG) lowering agents. Tinospora cordifolia (TC) is a well-known Ayurvedic Drug and a rich source of protoberberine alkaloids hence can contribute to TG lowering without side effects. Hence, to explore the therapeutic efficacy of T. cordifolia and its effects on biochemistry and metabolome in the patients of hyper-triglyceridemia, clinical trials were conducted. Methods Patients (n = 24) with hypertriglyceridemia were randomized into two groups to receive T. cordifolia extract (TCE) (3.0 g/per day) and metformin (850 mg/day) for 14 days having >300 mg/dl triglyceride level and cholesterol in the range of 130-230 mg/dl. Lipid profiles of blood samples were analyzed. Urine samples were subjected to HPLC-QTOF-MS to quantify oxidative damage and abnormal metabolic regulation. Results Intervention with TCE reduced the triglyceride, LDL, and VLDL levels to 380.45 ± 17.44, 133.25 ± 3.18, and 31.85 ± 5.88 mg/dL and increased the HDL to 47.50 ± 9.05 mg/dL significantly (p < 0.05) in the HG patients after 14 days treatment. TCE dosage potently suppressed the inflammatory and oxidative stress marker's i.e. levels of isoprostanes significantly (p < 0.01). Qualitative metabolomics approach i.e. PCA and PLS-DA showed significant alterations (p < 0.05) in the levels of 40 metabolites in the urine samples from different groups. Conclusion TCE administration depleted the levels of markers of HG i.e. VLDL, TG, and LDL significantly. Metabolomics studies established that the anti-HG activity of TCE was due to its antioxidative potential and modulation of the biopterin, butanoate, amino acid, and vitamin metabolism. Clinical trials registry India (CTRI) registration no. CTRI- 2016-08-007187.

Rajasekaran Chandrasekaran - One of the best experts on this subject based on the ideXlab platform.

  • Neuroprotective effect of Tagara, an Ayurvedic Drug against methyl mercury induced oxidative stress using rat brain mitochondrial fractions
    BMC Complementary and Alternative Medicine, 2015
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Background Methyl mercury (MeHg), an important environmental toxicant is implicated in neurological disorders such as Hunter-Russell syndrome and Autism. Therefore, the present work is in search of new Drugs that can alleviate MeHg toxicity. In this connection, Tagara, an Ayurvedic Drug is used for assessing its neuro protective effect against MeHg toxicity. Methods In the present study, we assessed the phytochemical contents of Tagara by colorimetric and HPLC analyses. The neuroprotective effect of Tagara on MeHg induced neurotoxicity was measured in terms of viability by MTT assay and oxidative stress in terms of catalase activity, glutathione and thiobarbituric acid reactive substance levels. Further, the chelating effect of Tagara towards MeHg was performed to identify the molecular mechanism. Statistical analysis was done by statistical package for social sciences (SPSS) version 16.0. Results The results demonstrated that Tagara contains significant amounts of phenols and flavonoids. Also, HPLC analysis of Tagara revealed the presence of essential oils such as hydroxyvalerenic and valerenic acids. Our results demonstrated that exposure of rat brain mitochondrial fractions to MeHg resulted in a dose dependent death in MTT assay and IC_50 value was found to be 10 μM. However, a 250 μg dose of Tagara effectively prevented MeHg induced mitochondrial damage. The oxidative stress caused by MeHg results in elevated levels of reactive oxygen species as evidenced by elevated TBARS (Thiobarbituric acid-reactive substances) levels and diminished catalase enzyme activity and glutathione content. However, Tagara at 250 μg concentration offsets these alterations caused by MeHg. Further, Tagara also diminished GSH oxidation caused by MeHg, confirming its chelating effect, one of the molecular mechanisms that triggers protection against oxidative damage. Conclusion Our results revealed that MeHg induced toxicity is predominantly mediated through oxidative stress mechanism and the propensity of Tagara to abolish such reactions. Hence, we propose that Tagara with a source of potential neuroprotectants may be a useful approach to alleviate MeHg associated neurotoxicity.

  • Neuroprotective effect of Tagara, an Ayurvedic Drug against methyl mercury induced oxidative stress using rat brain mitochondrial fractions
    BMC complementary and alternative medicine, 2015
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Methyl mercury (MeHg), an important environmental toxicant is implicated in neurological disorders such as Hunter-Russell syndrome and Autism. Therefore, the present work is in search of new Drugs that can alleviate MeHg toxicity. In this connection, Tagara, an Ayurvedic Drug is used for assessing its neuro protective effect against MeHg toxicity. In the present study, we assessed the phytochemical contents of Tagara by colorimetric and HPLC analyses. The neuroprotective effect of Tagara on MeHg induced neurotoxicity was measured in terms of viability by MTT assay and oxidative stress in terms of catalase activity, glutathione and thiobarbituric acid reactive substance levels. Further, the chelating effect of Tagara towards MeHg was performed to identify the molecular mechanism. Statistical analysis was done by statistical package for social sciences (SPSS) version 16.0. The results demonstrated that Tagara contains significant amounts of phenols and flavonoids. Also, HPLC analysis of Tagara revealed the presence of essential oils such as hydroxyvalerenic and valerenic acids. Our results demonstrated that exposure of rat brain mitochondrial fractions to MeHg resulted in a dose dependent death in MTT assay and IC50 value was found to be 10 μM. However, a 250 μg dose of Tagara effectively prevented MeHg induced mitochondrial damage. The oxidative stress caused by MeHg results in elevated levels of reactive oxygen species as evidenced by elevated TBARS (Thiobarbituric acid-reactive substances) levels and diminished catalase enzyme activity and glutathione content. However, Tagara at 250 μg concentration offsets these alterations caused by MeHg. Further, Tagara also diminished GSH oxidation caused by MeHg, confirming its chelating effect, one of the molecular mechanisms that triggers protection against oxidative damage. Our results revealed that MeHg induced toxicity is predominantly mediated through oxidative stress mechanism and the propensity of Tagara to abolish such reactions. Hence, we propose that Tagara with a source of potential neuroprotectants may be a useful approach to alleviate MeHg associated neurotoxicity.

  • Natural Product Research: Formerly Natural Product Letters
    2014
    Co-Authors: Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran, Kalaivani Thiagarajan
    Abstract:

    Dhanoop Manikoth Ayyathan, Rajasekaran Chandrasekaran & KalaivaniThiagarajan (2014): Neuroprotective effect of Brahmi, an Ayurvedic Drug against oxidative stressinduced by methyl mercury toxicity in rat brain mitochondrial-enriched fractions, Natural ProductResearch: Formerly Natural Product Letters, DOI: 10.1080/14786419.2014.968153

Amol Kadu - One of the best experts on this subject based on the ideXlab platform.

  • Comparative study of efficacy of Gunja Beeja lepa and Shunthi Churna lepa in Inflammatory Conditions of Arthritis - A Randomized Controlled Single Blinded Clinical Study
    International Journal of Ayurvedic Medicine, 2020
    Co-Authors: Nilima Wadnerwar, Prasad K S R, Meena Shamrao Deogade, Amol Kadu
    Abstract:

    Background: Life style disorders are an emerging problem in India where various types of arthritis are hampering the routine activities of people due to severe pain and inflammation. In such circumstances, everybody wants quick relief with the symptoms. Visha dravya used in Ayurveda are known for their quick action. Gunja (Abrus precatorius Linn) is a visha indicated in various diseases for internal and external use. Objectives: A randomized clinical study was conducted to assess the efficacy of Gunja beeja lepa to manage the local inflammatory conditions of Arthritis in comparison with a standard anti-inflammatory Ayurvedic Drug Shunthi (Zingiber officinalis Linn) to provide a potent anti-inflammatory Drug for the purpose of clinical practice. Material and methods: Patients suffering from transient mono or bi-arthropathies of knee were applied Gunja beeja lepa in comparison with local application of standard anti-inflammatory Ayurvedic Drug Shunthi. Assessment was done with the help of Disease Activity Score (DAS-28-3) including three variables viz; tender joint score (Range 0-28), Swollen Joint Count (Range 0-28) and Erythrocyte sedimentation rate. Statistical comparisons were performed by both paired, unpaired student’s t test by using Sigma stat software (version 3.1) for both the experimental the groups at p