Bacillus Amyloliquefaciens

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 6492 Experts worldwide ranked by ideXlab platform

Rainer Borriss - One of the best experts on this subject based on the ideXlab platform.

  • Malonylome of the plant growth promoting rhizobacterium with potent biocontrol activity, Bacillus Amyloliquefaciens FZB42
    Data in Brief, 2017
    Co-Authors: Ben Fan, Xiao-jun Peng, Rainer Borriss
    Abstract:

    The data presented in this article are related to the publication entitled "Malonylome analysis of rhizobacterium Bacillus Amyloliquefaciens FZB42 reveals involvement of lysine malonylation in polyketide synthesis and plant-bacteria interactions"(doi:10.1016/j.jprot.2016.11.022) (B. Fan, Y. Li, L. Li et al.) [1]. This article presented the raw information of all malonyllysine sites identified by LC-MS/MS in the Bacillus Amyloliquefaciens FZB42. Further, the functional features and conservation of the malonylated peptide/proteins were analyzed and made publicly available to enable critical or extended analyses.

  • drna seq reveals genomewide tsss and noncoding rnas of plant beneficial rhizobacterium Bacillus Amyloliquefaciens fzb42
    PLOS ONE, 2015
    Co-Authors: Ben Fan, Yanjie Chao, Konrad U Forstner, Jorg Vogel, Rainer Borriss
    Abstract:

    Bacillus Amyloliquefaciens subsp. plantarum FZB42 is a representative of Gram-positive plant-growth-promoting rhizobacteria (PGPR) that inhabit plant root environments. In order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain FZB42 grown under rhizosphere-mimicking conditions using differential RNA sequencing (dRNA-seq). Our analysis revealed 4,877 transcription start sites for protein-coding genes, identified genes differentially expressed under different growth conditions, and corrected many previously mis-annotated genes. We also identified a large number of riboswitches and cis-encoded antisense RNAs, as well as trans-encoded small noncoding RNAs that may play important roles in the gene regulation of Bacillus. Overall, our analyses provided a landscape of Bacillus primary transcriptome and improved the knowledge of rhizobacteria-host interactions.

  • difficidin and bacilysin from Bacillus Amyloliquefaciens fzb42 have antibacterial activity against xanthomonas oryzae rice pathogens
    Scientific Reports, 2015
    Co-Authors: Huijun Wu, Xinfang Yu, Lina Chen, Rainer Borriss
    Abstract:

    Bacterial blight and bacterial leaf streak are serious, economically damaging, diseases of rice caused by the bacteria Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola. Bacillus Amyloliquefaciens FZB42 was shown to possess biocontrol activity against these Xanthomonas strains by producing the antibiotic compounds difficidin and bacilysin. Analyses using fluorescence, scanning electron and transmission electron microscopy revealed difficidin and bacilysin caused changes in the cell wall and structure of Xanthomonas. Biological control experiments on rice plants demonstrated the ability of difficidin and bacilysin to suppress disease. Difficidin and bacilysin caused downregulated expression of genes involved in Xanthomonas virulence, cell division, and protein and cell wall synthesis. Taken together, our results highlight the potential of B. Amyloliquefaciens FZB42 as a biocontrol agent against bacterial diseases of rice, and the utility of difficidin and bacilysin as antimicrobial compounds.

  • biocontrol mechanism by root associated Bacillus Amyloliquefaciens fzb42 a review
    Frontiers in Microbiology, 2015
    Co-Authors: Soumitra Paul Chowdhury, Anton Hartmann, Xuewen Gao, Rainer Borriss
    Abstract:

    Bacillus Amyloliquefaciens subsp. plantarum FZB42 is a Gram-positive model bacterium for unraveling plant-microbe interactions in Bacilli. In addition, FZB42 is used commercially as biofertilizer and biocontrol agent in agriculture. Genome analysis of FZB42 revealed that nearly 10% of the FZB42 genome is devoted to synthesizing antimicrobial metabolites and their corresponding immunity genes. However, recent investigations in planta demonstrated that - except surfactin - the amount of such compounds found in vicinity of plant roots is relatively low, making doubtful a direct function in suppressing competing microflora including plant pathogens. These metabolites have been also suspected to induce changes within the rhizosphere microbial community, which might affect environment and plant health. However, sequence analysis of rhizosphere samples revealed only marginal changes in the root microbiome, suggesting that secondary metabolites are not the key factor in protecting plants from pathogenic microorganisms. On the other hand, adding FZB42 to plants compensate, at least in part, changes in the community structure caused by the pathogen, indicating an interesting mechanism of plant protection by beneficial Bacilli. Sub-lethal concentrations of cyclic lipopeptides and volatiles produced by plant-associated Bacilli trigger pathways of induced systemic resistance (ISR), which protect plants against attacks of pathogenic microbes, viruses, and nematodes. Stimulation of ISR by bacterial metabolites is likely the main mechanism responsible for biocontrol action of FZB42.

  • transcriptomic profiling of Bacillus Amyloliquefaciens fzb42 in response to maize root exudates
    BMC Microbiology, 2012
    Co-Authors: Ben Fan, Lilia C Carvalhais, Anke Becker, Dmitri Fedoseyenko, Nicolaus Von Wiren, Rainer Borriss
    Abstract:

    Background: Plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (PGPR) and their host plants. Most investigations were performed on Gram-negative rhizobacteria, while much less is known about Gram-positive rhizobacteria. To elucidate early responses of PGPR to root exudates, we investigated changes in the transcriptome of a Gram-positive PGPR to plant root exudates. Results: Bacillus Amyloliquefaciens FZB42 is a well-studied Gram-positive PGPR. To obtain a comprehensive overview of FZB42 gene expression in response to maize root exudates, microarray experiments were performed. A total of 302 genes representing 8.2% of the FZB42 transcriptome showed significantly altered expression levels in the presence of root exudates. The majority of the genes (261) was up-regulated after incubation of FZB42 with root exudates, whereas only 41 genes were down-regulated. Several groups of the genes which were strongly induced by the root exudates are involved in metabolic pathways relating to nutrient utilization, bacterial chemotaxis and motility, and non-ribosomal synthesis of antimicrobial peptides and polyketides. Conclusions: Here we present a transcriptome analysis of the root-colonizing bacterium Bacillus Amyloliquefaciens FZB42 in response to maize root exudates. The 302 genes identified as being differentially transcribed are proposed to be involved in interactions of Gram-positive bacteria with plants.

Qirong Shen - One of the best experts on this subject based on the ideXlab platform.

  • organic acids from root exudates of banana help root colonization of pgpr strain Bacillus Amyloliquefaciens njn 6
    Scientific Reports, 2015
    Co-Authors: Jun Yuan, Qiwei Huang, Jorge M Vivanco, Rong Li, Waseem Raza, Nan Zhang, Qirong Shen
    Abstract:

    Organic acids from root exudates of banana help root colonization of PGPR strain Bacillus Amyloliquefaciens NJN-6

  • enhanced control of cucumber wilt disease by Bacillus Amyloliquefaciens sqr9 by altering the regulation of its degu phosphorylation
    Applied and Environmental Microbiology, 2014
    Co-Authors: Zhihui Xu, Ruifu Zhang, Haichao Feng, Dandan Wang, Nan Zhang, Qirong Shen
    Abstract:

    Bacillus Amyloliquefaciens strain SQR9, isolated from the cucumber rhizosphere, suppresses the growth of Fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion through efficient root colonization. In the Gram-positive bacterium Bacillus, the response regulator DegU regulates genetic competence, swarming motility, biofilm formation, complex colony architecture, and protease production. In this study, we report that stepwise phosphorylation of DegU in B. Amyloliquefaciens SQR9 can influence biocontrol activity by coordinating multicellular behavior and regulating the synthesis of antibiotics. Results from in vitro and in situ experiments and quantitative PCR (qPCR) studies demonstrate the following: (i) that the lowest level of phosphorylated DegU (DegU∼P) (the degQ mutation) impairs complex colony architecture, biofilm formation, colonization activities, and biocontrol efficiency of Fusarium wilt disease but increases the production of macrolactin and bacillaene, and (ii) that increasing the level of DegU∼P by degQ and degSU overexpression significantly improves complex colony architecture, biofilm formation, colonization activities, production of the antibiotics bacillomycin D and difficidin, and efficiency of biocontrol of Fusarium wilt disease. The results offer a new strategy to enhance the biocontrol efficacy of Bacillus Amyloliquefaciens SQR9.

  • enhanced root colonization and biocontrol activity of Bacillus Amyloliquefaciens sqr9 by abrb gene disruption
    Applied Microbiology and Biotechnology, 2013
    Co-Authors: Jun Weng, Qirong Shen, Yang Wang, Juan Li, Ruifu Zhang
    Abstract:

    Root colonization by antagonistic bacteria is a prerequisite for successful biological control, and the instability of colonization under varying environmental conditions has accentuated the need to improve the colonization activity. Root colonization by Bacillus spp. is mainly determined by chemotaxis and biofilm formation, and both functions are negatively controlled by the global transcription regulator AbrB. Here, we disrupted the gene abrB in Bacillus Amyloliquefaciens SQR9, which has been proven to be a promising biocontrol agent of cucumber and watermelon wilt disease. Chemotaxis, biofilm formation, and colonization activities as well as biocontrol efficiency were measured and compared between the wild-type strain of SQR9 and the abrB mutant. The data presented in this article demonstrate that the colonization and biocontrol activity of B. Amyloliquefaciens SQR9 could be significantly improved by abrB gene disruption. The results offer a new strategy to enhance the biocontrol efficacy of B. Amyloliquefaciens SQR9.

  • contribution of bacillomycin d in Bacillus Amyloliquefaciens sqr9 to antifungal activity and biofilm formation
    Applied and Environmental Microbiology, 2013
    Co-Authors: Jiahui Shao, Qirong Shen, Xin Yan, Ruifu Zhang
    Abstract:

    Bacillus Amyloliquefaciens strains are capable of suppressing soilborne pathogens through the secretion of an array of lipopeptides and root colonization, and biofilm formation ability is considered a prerequisite for efficient root colonization. In this study, we report that one of the lipopeptide compounds (bacillomycin D) produced by the rhizosphere strain Bacillus Amyloliquefaciens SQR9 not only plays a vital role in the antagonistic activity against Fusarium oxysporum but also affects the expression of the genes involved in biofilm formation. When the bacillomycin D and fengycin synthesis pathways were individually disrupted, mutant SQR9M1, which was deficient in the production of bacillomycin D, only showed minor antagonistic activity against F. oxysporum, but another mutant, SQR9M2, which was deficient in production of fengycin, showed antagonistic activity equivalent to that of the wild-type strain of B. Amyloliquefaciens SQR9. The results from in vitro, root in situ, and quantitative reverse transcription-PCR studies demonstrated that bacillomycin D contributes to the establishment of biofilms. Interestingly, the addition of bacillomycin D could significantly increase the expression levels of kinC gene, but KinC activation is not triggered by leaking of potassium. These findings suggest that bacillomycin D contributes not only to biocontrol activity but also to biofilm formation in strain B. Amyloliquefaciens SQR9.

  • antifungal activity of Bacillus Amyloliquefaciens njn 6 volatile compounds against fusarium oxysporum f sp cubense
    Applied and Environmental Microbiology, 2012
    Co-Authors: Jun Yuan, Waseem Raza, Qirong Shen, Qiwei Huang
    Abstract:

    Bacillus Amyloliquefaciens NJN-6 produces volatile compounds (VOCs) that inhibit the growth and spore germination of Fusarium oxysporum f. sp. cubense. Among the total of 36 volatile compounds detected, 11 compounds completely inhibited fungal growth. The antifungal activity of these compounds suggested that VOCs can play important roles over short and long distances in the suppression of Fusarium oxysporum.

Hajime Unno - One of the best experts on this subject based on the ideXlab platform.

  • functional analysis of antibacterial activity of Bacillus Amyloliquefaciens phage endolysin against gram negative bacteria
    FEBS Letters, 2001
    Co-Authors: Masatomo Morita, Katsunori Mizoguchi, Aya Soejima, Yuji Orito, Yasunori Tanji, Hajime Unno
    Abstract:

    To analyze the antibacterial activity of Bacillus Amyloliquefaciens phage endolysin, nine deletion derivatives of the endolysin were constructed. Each deletion mutant was overexpressed, purified and characterized. The catalytic domain was located on the N-terminal region and the C-terminus had an affinity with the bacterial envelope. The enzymatic activity remained in spite of the deletion of the C-terminal 116-amino acid region; however, the antibacterial activity was lost. These results indicate that antibacterial action requires both the C-terminal cell-binding and the N-terminal enzymatic activities.

  • antibacterial activity of Bacillus Amyloliquefaciens phage endolysin without holin conjugation
    Journal of Bioscience and Bioengineering, 2001
    Co-Authors: Masatomo Morita, Katsunori Mizoguchi, Aya Soejima, Yuji Orito, Yasunori Tanji, Hajime Unno
    Abstract:

    To characterize the enzymatic activity and antibacterial activity of endolysin encoded by a Bacillus Amyloliquefaciens phage, the open reading frame encoding endolysin was amplified by PCR and cloned into the expression plasmid pET21d(+). The resultant plasmid was used to transform Escherichia coli JM109(DE3). Production of endolysin in the cytosol facilitated cell lysis without coproduction of holin, which is considered to degrade or alter the cytoplasmic membrane. The phage endolysin was overexpressed and purified. Although the specific activity of the purified phage endolysin towards lyophilized Micrococcus luteus cells was 111 of the activity of chicken egg white lysozymes, the endolysin showed stronger antibacterial activity towards E. coli W3110, E. coli JM109(DE3) and Pseudomonas aeruginosa PAO1 than chicken egg white lysozymes. The antibacterial activity of the endolysin towards these three bacterial strains was marked when EDTA was added to the endolysin solution.

Qiwei Huang - One of the best experts on this subject based on the ideXlab platform.

Jun Yuan - One of the best experts on this subject based on the ideXlab platform.

Bacillus Amyloliquefaciens - 15 days free trial to Access Experts & Abstracts