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Edward B Breitschwerdt - One of the best experts on this subject based on the ideXlab platform.
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Bartonella henselae in a dog with ear tip vasculitis
Veterinary Dermatology, 2018Co-Authors: Brittany L. Southern, Pradeep Neupane, Marna E. Ericson, Jamie C. Dencklau, Keith E. Linder, Julie M. Bradley, Gabriel P. Mckeon, Charles T. Long, Edward B BreitschwerdtAbstract:BACKGROUND Bartonella henselae, a Gram-negative, zoonotic, alpha-proteobacteria has been previously implicated in association with cutaneous vasoproliferative lesions (bacillary angiomatosis), nodular panniculitis and multifocal erythema (erythema multiforme) in dogs. OBJECTIVE Describe clinical, microbiological and histological lesions in a dog with ear margin vasculitis and B. henselae infection. ANIMALS A 12-month-old, specific pathogen-free intact female beagle dog maintained in a vector-free laboratory animal resource facility. METHODS AND MATERIALS Bartonella and Rickettsia serological evaluation, Bartonella and Rickettsia PCR, Bartonella alpha-proteobacteria growth medium (BAPGM) enrichment blood culture/PCR, histopathological investigation and confocal immunohistochemical evaluation. RESULTS Serological investigation (seroreversion) and PCR testing of aural tissue biopsies failed to support Rickettsia rickettsii as a cause of the aural vasculitis; however, B. henselae, genotype San Antonio 2 DNA was amplified and sequenced from both ear tip margins and from normal-appearing abdominal skin. Seroconversion to B. henselae was documented retrospectively by IFA testing. Bartonella henselae organisms were visualized by confocal immunostaining within all three biopsies. Histopathology revealed small vessel necrotizing vasculitis and dermal necrosis. Bartonella henselae seroreversion and complete resolution of skin lesions occurred in conjunction with administration of oral doxycycline and enrofloxacin for six weeks. CONCLUSIONS AND CLINICAL IMPORTANCE Bartonella henselae is an emerging zoonotic pathogen that has been associated with leucocytoclastic vasculitis in humans and may have had a contributing or causative role in the development of the cutaneous aural margin vasculitis in this beagle.
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Bartonella henselae in a dog with ear tip vasculitis.
Veterinary dermatology, 2018Co-Authors: Brittany L. Southern, Pradeep Neupane, Marna E. Ericson, Jamie C. Dencklau, Keith E. Linder, Julie M. Bradley, Gabriel P. Mckeon, Charles T. Long, Edward B BreitschwerdtAbstract:Bartonella henselae, a Gram-negative, zoonotic, alpha-proteobacteria has been previously implicated in association with cutaneous vasoproliferative lesions (bacillary angiomatosis), nodular panniculitis and multifocal erythema (erythema multiforme) in dogs. Describe clinical, microbiological and histological lesions in a dog with ear margin vasculitis and B. henselae infection. A 12-month-old, specific pathogen-free intact female beagle dog maintained in a vector-free laboratory animal resource facility. Bartonella and Rickettsia serological evaluation, Bartonella and Rickettsia PCR, Bartonella alpha-proteobacteria growth medium (BAPGM) enrichment blood culture/PCR, histopathological investigation and confocal immunohistochemical evaluation. Serological investigation (seroreversion) and PCR testing of aural tissue biopsies failed to support Rickettsia rickettsii as a cause of the aural vasculitis; however, B. henselae, genotype San Antonio 2 DNA was amplified and sequenced from both ear tip margins and from normal-appearing abdominal skin. Seroconversion to B. henselae was documented retrospectively by IFA testing. Bartonella henselae organisms were visualized by confocal immunostaining within all three biopsies. Histopathology revealed small vessel necrotizing vasculitis and dermal necrosis. Bartonella henselae seroreversion and complete resolution of skin lesions occurred in conjunction with administration of oral doxycycline and enrofloxacin for six weeks. Bartonella henselae is an emerging zoonotic pathogen that has been associated with leucocytoclastic vasculitis in humans and may have had a contributing or causative role in the development of the cutaneous aural margin vasculitis in this beagle. © 2018 ESVD and ACVD.
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Splenic vasculitis, thrombosis, and infarction in a febrile dog infected with Bartonella henselae
Journal of veterinary emergency and critical care (San Antonio Tex. : 2001), 2015Co-Authors: Steven G. Friedenberg, Nandhakumar Balakrishnan, Julien Guillaumin, Edward S. Cooper, Kristin Lewis, Duncan S. Russell, Edward B BreitschwerdtAbstract:Objective To describe the clinical course and successful management of a febrile dog with polyarthritis, splenic vasculitis, thrombosis, and infarction that was infected with Bartonella henselae. Case Summary An 8-year-old female spayed Labrador Retriever was referred to The Ohio State University Veterinary Medical Center Emergency Service for evaluation of limping, fever, vomiting, and malaise of 4 days’ duration. Physical examination abnormalities included generalized weakness, diminished conscious proprioception, bilateral temporalis muscle atrophy, and diarrhea. Peripheral lymph nodes were normal, and there were no signs of abdominal organomegaly, joint effusion, or spinal pain. Abdominal ultrasound identified a nonocclusive splenic vein thrombus. Fine-needle aspirates of the spleen revealed pyogranulomatous inflammation, mild reactive lymphoid hyperplasia, and mild extramedullary hematopoiesis. Splenic histopathology found marked, multifocal to coalescing acute coagulation necrosis (splenic infarctions) and fibrinoid necrotizing vasculitis. Bartonella henselae DNA was amplified by polymerase chain reaction and sequenced from the splenic tissue. The dog responded favorably to antimicrobials and was healthy at the time of follow-up evaluation. New and Unique Information Provided Bartonella henselae is an incompletely characterized emerging canine pathogen. This case report establishes a potential role for this bacterium as a cause of vasculitis and thromboembolism, which have not been previously reported in association with B. henselae infection in dogs.
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Infection with Bartonella henselae in a Danish Family
Journal of clinical microbiology, 2015Co-Authors: Ricardo G. Maggi, Nandhakumar Balakrishnan, Julie M. Bradley, Edward B BreitschwerdtAbstract:ABSTRACT Bartonella species constitute emerging, vector-borne, intravascular pathogens that produce long-lasting bacteremia in reservoir-adapted (natural host or passive carrier of a microorganism) and opportunistic hosts. With the advent of more sensitive and specific diagnostic tests, there is evolving microbiological evidence supporting concurrent infection with one or more Bartonella spp. in more than one family member; however, the mode(s) of transmission to or among family members remains unclear. In this study, we provide molecular microbiological evidence of Bartonella henselae genotype San Antonio 2 (SA2) infection in four of six Danish family members, including a child who died of unknown causes at 14 months of age.
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Concurrent Bartonella henselae infection in a dog with panniculitis and owner with ulcerated nodular skin lesions
Veterinary dermatology, 2014Co-Authors: Michael A. Rossi, Nandhakumar Balakrishnan, Keith E. Linder, Jacqueline B. Messa, Edward B BreitschwerdtAbstract:Bartonella henselae, a Gram-negative, zoonotic Alphaproteobacteria that infects erythrocytes, endothelial cells and dendritic cells, has previously been implicated as a cause of panniculitis in dogs and a human. An 8-year-old, spayed female Labrador retriever and its 78-year-old male owner living in the same household. When preliminary and advanced testing failed to identify the cause of near-simultaneous-onset dermatological lesions, Bartonella serology, Bartonella Alphaproteobacteria growth medium (BAPGM) enrichment blood culture/PCR and immunohistochemistry were used to test specimens from the dog and owner. Bartonella henselae, genotype San Antonio 2 DNA was amplified and sequenced from the man's BAPGM enrichment blood culture and the dog's panniculitis lesion. The bacterium was visualized by immunohistochemistry in the dog's panniculitis lesion; however, neither the dog nor the owner was B. henselae seroreactive. Antibiotic therapy elicited dermatological improvement in both dog and owner. Bartonella henselae is an emerging zoonotic pathogen that induces granulomatous inflammatory lesions in various tissues of animals, including humans. We conclude that this bacterium had a contributory or causative role in the development of the dermatological lesions in the dog and owner. © 2014 ESVD and ACVD.
Christoph Dehio - One of the best experts on this subject based on the ideXlab platform.
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Interactions of Bartonella henselae with vascular endothelial cells.
Current opinion in microbiology, 1999Co-Authors: Christoph DehioAbstract:The emerging human pathogen Bartonella henselae has the remarkable capacity to colonise vascular tissues and to stimulate vasoproliferative tumour growth. Although the molecular principle of bacterium-induced neovascularisation (angiogenesis) is still unclear, recent studies have indicated a novel mechanism of endothelial colonisation that involves the formation, engulfment and uptake of a large bacterial aggregate.
Giuseppe Maggiore - One of the best experts on this subject based on the ideXlab platform.
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Bartonella henselae infection associated with Guillain-Barre syndrome.
The Pediatric infectious disease journal, 2006Co-Authors: Francesco Massei, Pierantonio Macchia, Laura Gori, Grazia Taddeucci, Giuseppe MaggioreAbstract:This is the first report of Guillain-Barre syndrome (GBS) related to Bartonella henselae infection. A 10-year-old girl had difficulty walking and marked myalgia. The search for all causes known to trigger GBS was negative. She was treated with intravenous immunoglobulins and recovered. Because she lived in a rural area and had a history of kitten contact, a specific serology for B. henselae infection was performed and confirmed an ongoing infection. She did not show any clinical typical feature of cat-scratch disease. B. henselae infection should be considered in the wide etiologic spectrum of GBS.
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Pseudoinfectious mononucleosis: a presentation of Bartonella henselae infection.
Archives of disease in childhood, 2000Co-Authors: Francesco Massei, M Massimetti, Francesco Messina, Pierantonio Macchia, Giuseppe MaggioreAbstract:Six children presented during one year with clinical features of infectious mononucleosis, but with laboratory findings of leucocytosis with neutrophilia, increased erythrocyte sedimentation rate, and hypergammaglobulinaemia. Serology for Epstein-Barr virus, cytomegalovirus, adenovirus, and Toxoplasma gondii was negative, while anti-Bartonella henselae IgM with high IgG titre (>/=1/1024) was present in all. All children had contact with kittens. No specific treatment was administered and all recovered.
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Bartonella henselae and inflammatory bowel disease.
Lancet (London England), 2000Co-Authors: Francesco Massei, M Massimetti, Francesco Messina, Pierantonio Macchia, Giuseppe MaggioreAbstract:Sustained fever and increased thickness of the distal ileum on ultrasound suggested Crohn's disease in an adolescent boy. Bartonella henselae infection was diagnosed by specific serology and the patient recovered. Ileitis could be related to B. henselae infection.
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Widening of the clinical spectrum of Bartonella henselae infection as recognized through serodiagnostics
European Journal of Pediatrics, 2000Co-Authors: Francesco Massei, Francesco Messina, Pierantonio Macchia, Ilaria Talini, Mauro Massimetti, Gabriella Palla, Giuseppe MaggioreAbstract:The recently improved diagnostics have widened, in children, the spectrum of clinical manifestations recognisable as Bartonella henselae infection. We report here the clinical features of 20 (14 males) consecutive children with serologically proved B. henselae infection observed within 12 months in the Paediatric Department of the University of Pisa. The patients had a mean age of 7 years 4 months (range 1.1–14.1 years). All children but one had a history of contact with kittens. Clinical manifestations included regional lymphadenopathy in 14 patients, representing in five the only clinical manifestation at onset, infectious mononucleosis-like syndrome in six, erythema nodosum in three, and Parinaud oculoglandular syndrome in one. In five patients a severe disorder was first suspected: fever of unknown origin in two with multiple hepatosplenic granulomatosis in one; osteolityc lesion suggesting bone neoplasm, marked inguinal lymph-node enlargement, suggesting Burkitt lymphoma, and an acute encephalopathy in one each. Bartonella henselae IgG antibody was positive in all patients with a titre ranging from 1:128 to 1:8590. IgM antibody was present in all except one child with an IgG titre of 1:2048. All patients recovered, some spontaneously. Conclusion Bartonella henselae infection is frequent in Tuscany and probably underdiagnosed due to the high frequency of atypical onset of the clinical manifestations. An accurate clinical history and a reasonably wide use of the serological test may allow a rapid and accurate diagnosis, reassuring the family of the patient and avoiding invasive and expensive diagnostic procedures.
Hidehiro Tsuneoka - One of the best experts on this subject based on the ideXlab platform.
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Bartonella henselae DNA in Seronegative Patients with Cat-Scratch Disease.
Emerging infectious diseases, 2018Co-Authors: Masashi Yanagihara, Hidehiro Tsuneoka, Ayano Tanimoto, Ken-ichiro Otsuyama, Jun Nishikawa, Tomohiro Matsui, Junzo Nojima, Kiyoshi IchiharaAbstract:We used real-time PCR to detect Bartonella henselae DNA in 7.9% (5/63) of blood specimens from seronegative patients in Japan suspected of having cat-scratch disease. The combined use of serologic tests and real-time PCR to analyze blood specimens is recommended for the prompt, noninvasive laboratory diagnosis of cat-scratch disease.
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Multispacer Typing of Bartonella henselae Isolates from Humans and Cats, Japan
Emerging infectious diseases, 2010Co-Authors: Masashi Yanagihara, Hidehiro Tsuneoka, Junzo Nojima, Motoki Sugasaki, Kiyoshi IchiharaAbstract:To determine genotypic distribution of and relationship between human and cat strains of Bartonella henselae,we characterized 56 specimens using multispacer typing (MST). Of 13 MST genotypes identified, 12 were grouped into cluster 1. In Japan, human infections can be caused by B. henselae strains in cluster 1.
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Bartonella henselae Infection as a Cause of Fever of Unknown Origin
Journal of clinical microbiology, 2000Co-Authors: Masato Tsukahara, Ichiro Murano, Hidehiro Tsuneoka, Hidechika Iino, Hirohiko Takahashi, Masashi UchidaAbstract:Fourteen of 41 patients (34%) with a serological diagnosis of Bartonella henselae infection were found to have prolonged fever or fever of unknown origin, suggesting that generalized systemic B. henselae infection is not rare in immunocompetent healthy individuals.
Didier Raoult - One of the best experts on this subject based on the ideXlab platform.
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Bartonella henselae in skin biopsy specimens of patients with cat scratch disease
Emerging Infectious Diseases, 2010Co-Authors: Emmanouil Angelakis, Sophie Edouard, Bernard La Scola, Didier RaoultAbstract:During the past 2 years, we identified live Bartonella henselae in the primary inoculation sites of 3 patients after a cat scratch. Although our data are preliminary, we report that a cutaneous swab of the skin lesion from a patient in the early stage of cat-scratch disease can be useful for diagnosis of the infection.
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Scalp Eschar and Neck Lymphadenopathy Caused by Bartonella henselae after Tick Bite
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2010Co-Authors: Emmanouil Angelakis, Sophie Edouard, Cristina Socolovschi, Celine Pulcini, Julie Waton, Patrick Imbert, P Dellamonica, Didier RaoultAbstract:Rickettsia slovaca and Rickettsia raoultii have been associated with a syndrome characterized by scalp eschar and neck lymphadenopathy following tick bites. However, in many cases, the causative agent remains undetermined. We report 3 cases of this syndrome caused by Bartonella henselae, and we propose the term "SENLAT" to collectively describe this clinical entity.
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Molecular Characterization of Resistance to Macrolides in Bartonella henselae
Antimicrobial agents and chemotherapy, 2006Co-Authors: Silpak Biswas, Didier Raoult, Jean-marc RolainAbstract:We selected in vitro erythromycin-resistant strains of Bartonella henselae. The mutants obtained had point mutations in domain V of 23S rRNA and/or in ribosomal protein L4. One lymph node of a patient with cat-scratch disease had such a mutation in 23S rRNA, suggesting that natural resistant strains may infect humans.
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Immunofluorescent detection of intraerythrocytic Bartonella henselae in naturally infected cats.
Journal of clinical microbiology, 2001Co-Authors: Jean-marc Rolain, B. La Scola, Zhongxing Liang, Bernard Davoust, Didier RaoultAbstract:To determine the presence of Bartonella henselae bacteremia in six cats, we compared isolation using blood culture with direct immunofluorescence on blood smears. Three cats that were positive by blood culture were also positive by direct immunofluorescence, and laser confocal microscopy confirmed the intraerythrocytic location of B. henselae.
