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Maíra De Freitas Pereira - One of the best experts on this subject based on the ideXlab platform.
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The transcriptional landscape of basidiosporogenesis in mature Pisolithus microcarpus basidiocarp
BMC Genomics, 2017Co-Authors: Maíra De Freitas Pereira, Francis Martin, André Narvaes Da Rocha Campos, Thalita Cardoso Anastacio, Emmanuelle Morin, Sérgio Hermínio Brommonschenkel, Annegret Kohler, Maurício Dutra CostaAbstract:Pisolithus microcarpus (Cooke & Massee) G. Cunn is a gasteromycete that produces closed basidiocarps in symbiosis with eucalypts and acacias. The fungus produces a complex basidiocarp composed of peridioles at different developmental stages and an upper layer of Basidiospores free of the hyphae and ready for wind dispersal upon the rupture of the basidiocarp pellis. During basidiosporogenesis, a process that takes place inside the basidiocarp peridioles, a conspicuous reserve of fatty acids is present throughout development. While several previous studies have described basidiosporogenesis inside peridioles, very little is known about gene expression changes that may occur during this part of the fungal life cycle. The objective of this work was to analyze gene transcription during peridiole and Basidiospore development, while focusing specifically on cell cycle progression and lipid metabolism. Throughout different developmental stages of the peridioles we analyzed, 737 genes were regulated between adjacent compartments (>5 fold, FDR-corrected p-value
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the transcriptional landscape of basidiosporogenesis in mature pisolithus microcarpus basidiocarp
BMC Genomics, 2017Co-Authors: Francis Martin, Maíra De Freitas Pereira, André Narvaes Da Rocha Campos, Thalita Cardoso Anastacio, Emmanuelle Morin, Sérgio Hermínio Brommonschenkel, Annegret Kohler, Maurício Dutra CostaAbstract:Pisolithus microcarpus (Cooke & Massee) G. Cunn is a gasteromycete that produces closed basidiocarps in symbiosis with eucalypts and acacias. The fungus produces a complex basidiocarp composed of peridioles at different developmental stages and an upper layer of Basidiospores free of the hyphae and ready for wind dispersal upon the rupture of the basidiocarp pellis. During basidiosporogenesis, a process that takes place inside the basidiocarp peridioles, a conspicuous reserve of fatty acids is present throughout development. While several previous studies have described basidiosporogenesis inside peridioles, very little is known about gene expression changes that may occur during this part of the fungal life cycle. The objective of this work was to analyze gene transcription during peridiole and Basidiospore development, while focusing specifically on cell cycle progression and lipid metabolism. Throughout different developmental stages of the peridioles we analyzed, 737 genes were regulated between adjacent compartments (>5 fold, FDR-corrected p-value < 0.05) corresponding to 3.49% of the genes present in the P. microcarpus genome. We identified three clusters among the regulated genes which showed differential expression between the peridiole developmental stages and the Basidiospores. During peridiole development, transcripts for proteins involved in cellular processes, signaling, and information storage were detected, notably those for coding transcription factors, DNA polymerase subunits, DNA repair proteins, and genes involved in chromatin structure. For both internal embedded Basidiospores (hereto referred to as “Internal spores”, IS) and external free Basidiospores (hereto referred to as “Free spores”, FS), upregulated transcripts were found to involve primary metabolism, particularly fatty acid metabolism (FA). High expression of transcripts related to β-oxidation and the glyoxylate shunt indicated that fatty acids served as a major carbon source for basidiosporogenesis. Our results show that basidiocarp formation in P. microcarpus involves a complex array of genes that are regulated throughout peridiole development. We identified waves of transcripts with coordinated regulation and identified transcription factors which may play a role in this regulation. This is the first work to describe gene expression patterns during basidiocarp formation in an ectomycorrhizal gasteromycete fungus and sheds light on genes that may play important roles in the developmental process.
Maurício Dutra Costa - One of the best experts on this subject based on the ideXlab platform.
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The transcriptional landscape of basidiosporogenesis in mature Pisolithus microcarpus basidiocarp
BMC Genomics, 2017Co-Authors: Maíra De Freitas Pereira, Francis Martin, André Narvaes Da Rocha Campos, Thalita Cardoso Anastacio, Emmanuelle Morin, Sérgio Hermínio Brommonschenkel, Annegret Kohler, Maurício Dutra CostaAbstract:Pisolithus microcarpus (Cooke & Massee) G. Cunn is a gasteromycete that produces closed basidiocarps in symbiosis with eucalypts and acacias. The fungus produces a complex basidiocarp composed of peridioles at different developmental stages and an upper layer of Basidiospores free of the hyphae and ready for wind dispersal upon the rupture of the basidiocarp pellis. During basidiosporogenesis, a process that takes place inside the basidiocarp peridioles, a conspicuous reserve of fatty acids is present throughout development. While several previous studies have described basidiosporogenesis inside peridioles, very little is known about gene expression changes that may occur during this part of the fungal life cycle. The objective of this work was to analyze gene transcription during peridiole and Basidiospore development, while focusing specifically on cell cycle progression and lipid metabolism. Throughout different developmental stages of the peridioles we analyzed, 737 genes were regulated between adjacent compartments (>5 fold, FDR-corrected p-value
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the transcriptional landscape of basidiosporogenesis in mature pisolithus microcarpus basidiocarp
BMC Genomics, 2017Co-Authors: Francis Martin, Maíra De Freitas Pereira, André Narvaes Da Rocha Campos, Thalita Cardoso Anastacio, Emmanuelle Morin, Sérgio Hermínio Brommonschenkel, Annegret Kohler, Maurício Dutra CostaAbstract:Pisolithus microcarpus (Cooke & Massee) G. Cunn is a gasteromycete that produces closed basidiocarps in symbiosis with eucalypts and acacias. The fungus produces a complex basidiocarp composed of peridioles at different developmental stages and an upper layer of Basidiospores free of the hyphae and ready for wind dispersal upon the rupture of the basidiocarp pellis. During basidiosporogenesis, a process that takes place inside the basidiocarp peridioles, a conspicuous reserve of fatty acids is present throughout development. While several previous studies have described basidiosporogenesis inside peridioles, very little is known about gene expression changes that may occur during this part of the fungal life cycle. The objective of this work was to analyze gene transcription during peridiole and Basidiospore development, while focusing specifically on cell cycle progression and lipid metabolism. Throughout different developmental stages of the peridioles we analyzed, 737 genes were regulated between adjacent compartments (>5 fold, FDR-corrected p-value < 0.05) corresponding to 3.49% of the genes present in the P. microcarpus genome. We identified three clusters among the regulated genes which showed differential expression between the peridiole developmental stages and the Basidiospores. During peridiole development, transcripts for proteins involved in cellular processes, signaling, and information storage were detected, notably those for coding transcription factors, DNA polymerase subunits, DNA repair proteins, and genes involved in chromatin structure. For both internal embedded Basidiospores (hereto referred to as “Internal spores”, IS) and external free Basidiospores (hereto referred to as “Free spores”, FS), upregulated transcripts were found to involve primary metabolism, particularly fatty acid metabolism (FA). High expression of transcripts related to β-oxidation and the glyoxylate shunt indicated that fatty acids served as a major carbon source for basidiosporogenesis. Our results show that basidiocarp formation in P. microcarpus involves a complex array of genes that are regulated throughout peridiole development. We identified waves of transcripts with coordinated regulation and identified transcription factors which may play a role in this regulation. This is the first work to describe gene expression patterns during basidiocarp formation in an ectomycorrhizal gasteromycete fungus and sheds light on genes that may play important roles in the developmental process.
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Histochemistry and storage of organic compounds during basidiosporogenesis in the ectomycorrhizal fungus Pisolithus microcarpus
World Journal of Microbiology and Biotechnology, 2010Co-Authors: André Narvaes Da Rocha Campos, Maurício Dutra CostaAbstract:Knowledge on the distribution and storage of different organic compounds during basidiosporogenesis in P. microcarpus is paramount to a better understanding of Basidiospore recalcitrance to germination. The objective of this work was to detect the presence and distribution of phenolics, reducing sugars, starch, glycogen, total polysaccharides, RNA, and proteins during P. microcarpus basidiosporogenesis . Starch and reducing sugars were not detected in the fungal basidiocarps, while other polysaccharides predominated in the extracellular matrix at the base of the basidiocarp containing unconsolidated peridioles. Phenolics were also detected in this region. Glycogen was present inside the hyphae, basidia, and Basidiospores and constitutes an important storage compound in the fungal basidiocarps. In mature Basidiospores, RNA accumulation occurred at discrete locations in the cytoplasmatic periphery, while polysaccharides and proteins were shown to predominate in the cell wall. The presence of glycogen, RNA, and proteins inside the Basidiospores strongly indicates provision for future germination and suggests that other factors may also influence Basidiospore recalcitrance.
Bruce A Auld - One of the best experts on this subject based on the ideXlab platform.
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teliospore germination Basidiospore formation and the infection process of puccinia xanthii on xanthium occidentale
Fungal Biology, 1992Co-Authors: L Morin, J F Brown, Bruce A AuldAbstract:Teliospore germination, Basidiospore formation and the infection process of Puccinia xanthii on Xanthium occidentale were examined using light and scanning electron microscopy. Telisopores germinated within 30 min after hydration. The germ-tube differentiated into a four-celled metabasidium within 4 h of exposure to high relative humidity. A Basidiospore was formed at the tip of a conical sterigma arising from each cell. Mature Basidiospores left on the telial surface often produced secondary Basidiospores. Basidiospores germinated by producing a single unbranched germ-tube of variable length on the leaf surface. The end of the germ-tube sometimes differentiated into an appressorium. Host penetration through the epidermis occurred at 3 to 6 h after inoculation and was associated with the formation of an intraepidermal vesicle. A septum was formed between the intraepidermal vesicle and the primary hypha at 15 h after inoculation. Primary hyphae ramified through the intercellular spaces of the palisade parenchyma cells or penetrated adjacent epidermal cells. The first terminal intracellular hypha (M haustorium) was observed at 24 h after inoculation. The terminal intracellular hypha arose from an unspecialized intercalary or terminal mother cell. Terminal intracellular hyphae showed considerable variation in shape and never grew out of the infected host's mesophyll cells.
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effects of environmental factors on teliospore germination germination Basidiospore formation and infection of xanthium occidentale by puccinia xanthii
Phytopathology, 1992Co-Authors: L Morin, J F Brown, Bruce A AuldAbstract:The effects of temperature, dew, high relative humidity, and light regimes on teliospore germination, Basidiospore production and germination, and infection of Noogoora burr (Xanthium occidentale) by Puccinia xanthii were examined. The optimum temperature for the germination of teliospores was between 20 and 30 C, and the optimum temperature for the production and germination of Basidiospores was 20 C. The total number of Basidiospores produced increased as the period under conditions of high relative humidity was increased. The highest levels of infection were observed at temperatures of 20 and 25 C []
Brandon P Matheny - One of the best experts on this subject based on the ideXlab platform.
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Basidiospore homoplasy and variation in the inocybe chelanensis group in north america
Mycologia, 2004Co-Authors: Bradley R Kropp, Brandon P MathenyAbstract:We present a morphological and phylo- genetic study of Inocybe chelanensis and other North American species of Inocybe that have unusually elon- gated nodulose spores. Taxonomy and illustrations of these species are provided, along with a key to these and similar species found in Europe and North America. The species concept of I. chelanensis is broadened to include the range of variation occur- ring for the species in North America. Despite similar Basidiospore morphologies, I. chelanensis and I. can- didipes are not closely related. Inocybe chelanensis is related more closely to I. stellatospora and I. candi- dipes is related to I. glabrodisca based on RPB1 and nLSU-rDNA nucleotide sequences. Distal enlonga- tion of Inocybe Basidiospores was achieved indepen- dently in at least two separate lineages of Inocybe. In- ocybe candidipes and I. sierraensis are described as new.
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Basidiospore homoplasy and variation in the inocybe chelanensis group in
2004Co-Authors: Bradley R Kropp, Brandon P MathenyAbstract:We present a morphological and phylo- genetic study of Inocybe chelanensis and other North American species of Inocybe that have unusually elon- gated nodulose spores. Taxonomy and illustrations of these species are provided, along with a key to these and similar species found in Europe and North America. The species concept of I. chelanensis is broadened to include the range of variation occur- ring for the species in North America. Despite similar Basidiospore morphologies, I. chelanensis and I. can- didipes are not closely related. Inocybe chelanensis is related more closely to I. stellatospora and I. candi- dipes is related to I. glabrodisca based on RPB1 and nLSU-rDNA nucleotide sequences. Distal enlonga- tion of Inocybe Basidiospores was achieved indepen- dently in at least two separate lineages of Inocybe. In- ocybe candidipes and I. sierraensis are described as new.
Shigeru Kaneko - One of the best experts on this subject based on the ideXlab platform.
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nuclear behavior during Basidiospore germination in cronartium quercuum f sp fusiforme
Mycologia, 1996Co-Authors: P C Spaine, Shigeru KanekoAbstract:Nuclear behavior during Basidiospore ger- mination in Cronartium quercuum f. sp. fusiforme was examined on glass slides and host seedlings using 4, 6-diamidino-2-phenylindole staining. Mononucleate Basidiospores of Cronartium quercuum f. sp. fusiforme normally were produced following meiosis in the te- liospore. However, a subsequent mitotic division of- ten occurred within each Basidiospore resulting in a short-lived binucleate condition. Within 1 h after
