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C.t. Ingold - One of the best experts on this subject based on the ideXlab platform.
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The Cintractia pattern of teliospore germination
Mycological Research, 1999Co-Authors: C.t. IngoldAbstract:Teliospore germination in Cintractia limitata and C. mitchellii is illustrated and shown to agree closely with that earlier reported in two other species of the genus. Basidial cells conjugate pairwise, and from each united pair a relatively large, and probably dikaryotic, blastoconidium is produced. In C. mitchellii, as a rare event, conjugation does not occur and cells of the Basidium form small basidiospores. It is pointed out the course of teliospore germination in Cintractia spp. departs markedly from the standard Ustilago type.
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Unusual teliospore germination in Ustilago triodiae and Sporisorium loudetiae-pedicellatae
Mycological Research, 1999Co-Authors: C.t. IngoldAbstract:The products of teliospore germination in Ustilago triodiae and Sporisorium loudetiae-pedicellatae are illustrated. In U. triodiae the transversely-septate Basidium is usually three-spored. The sporidia (basidiospores) either bud or form surface hyphae from which branched systems of aerial sporidia arise. A similar haplophase occurs in some other species.
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The Basidium in Ustilaginaceae
Mycologist, 1998Co-Authors: C.t. IngoldAbstract:This essay summarizes many recent papers by the author dealing with the Basidium in smut fungi belonging to Ustilaginaceae. In nearly all species, germination of the teliospore (proBasidium) either leads to a structure that is obviously a 4-celled linear Basidium or can be interpreted as a modification thereof.
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The basidia of Sporisorium formosanum and Ustilago affinis
Mycological Research, 1997Co-Authors: C.t. IngoldAbstract:The production of basidia from individual teliospores of Sporisorium formosanum and Ustilago affinis is described. In S. formosanum the upper portion of the Basidium separates as a conidium-like body the two cells of which either conjugate or produce sporidia, whilst the lower portion remains unicellular and gives rise to sporidia. In U. affinis the Basidium is four-celled and remains intact, its cells either conjugating or producing sporidia, sometimes on the same Basidium. These patterns of teliospore germination are compared with one another and other members of the Ustilaginaceae, notably Ustilago trichophora .
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Teliospore germination in Tilletia opaca and T. sumatii and the nature of the tilletiaceous Basidium
Mycological Research, 1997Co-Authors: C.t. IngoldAbstract:The process is described of teliospore germination in Tilletia opaca and T. sumatii leading to the production of basidiospores on basidia, and later the development of ballistospores and blastospores on agar. In both species the Basidium shows some evidence of being a condensed dichotomously branched structure. The situation in these two smuts is discussed in relation to that in several other species of Tilletia , and some consideration is given to the origirt of the Basidium and evolutionary trends within the genus.
Teruyuki Matsumoto - One of the best experts on this subject based on the ideXlab platform.
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karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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Karyological characterization of meiosis, post-meiotic mitosis and nuclear migration in the ectomycorrhizal fungus Rhizopogon roseolus (= R. rubescens)
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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short title rhizopogon roseolus karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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ultrastructure of developing basidiospores in rhizopogon roseolus r rubescens
Mycoscience, 2008Co-Authors: Norihiro Shimomura, Tadanori Aimi, Teruyuki Matsumoto, Nitaro Maekawa, Hiroshi OtaniAbstract:The ultrastructure of developing basidiospores in Rhizopogon roseolus is described. When viewed in the fruiting body chamber using scanning electron microscopy, basidiospores appear narrowly ellipsoid and have smooth walls. Eight basidiospores are usually produced on the apex of each sterigma on the Basidium. Transmission electron micrographs showed that basidiospores formed by movement of cytoplasm (including the nuclei) via the sterigmata, and then each basidiospore eventually became separated from its sterigma by an electron-lucent septum. The sterigma and Basidium subsequently collapsed, resulting in spore release. Freshly released spores retained the sterigmal appendage connected to the collapsed Basidium. After spore release, the major ultrastructural changes in the spore concerned the lipid bodies and the spore wall. During maturation, lipid bodies formed and then expanded. Before release, the spore wall was homogeneous and electronlucent, but after release the spore wall comprised two distinct layers with electron-dense depositions at the inner wall, and the dense depositions formed an electron-dense third layer. The mature spore wall complex comprised at least four distinct layers: the outer electron-lucent thin double layers, the mottled electron-dense third layer, and the electron-lucent fourth layer in which electron-lucent granular substances were dispersed.
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Ultrastructure of developing basidiospores in Rhizopogon roseolus (= R. rubescens)
Mycoscience, 2008Co-Authors: Norihiro Shimomura, Tadanori Aimi, Teruyuki Matsumoto, Nitaro Maekawa, Hiroshi OtaniAbstract:The ultrastructure of developing basidiospores in Rhizopogon roseolus is described. When viewed in the fruiting body chamber using scanning electron microscopy, basidiospores appear narrowly ellipsoid and have smooth walls. Eight basidiospores are usually produced on the apex of each sterigma on the Basidium. Transmission electron micrographs showed that basidiospores formed by movement of cytoplasm (including the nuclei) via the sterigmata, and then each basidiospore eventually became separated from its sterigma by an electron-lucent septum. The sterigma and Basidium subsequently collapsed, resulting in spore release. Freshly released spores retained the sterigmal appendage connected to the collapsed Basidium. After spore release, the major ultrastructural changes in the spore concerned the lipid bodies and the spore wall. During maturation, lipid bodies formed and then expanded. Before release, the spore wall was homogeneous and electronlucent, but after release the spore wall comprised two distinct layers with electron-dense depositions at the inner wall, and the dense depositions formed an electron-dense third layer. The mature spore wall complex comprised at least four distinct layers: the outer electron-lucent thin double layers, the mottled electron-dense third layer, and the electron-lucent fourth layer in which electron-lucent granular substances were dispersed.
Norihiro Shimomura - One of the best experts on this subject based on the ideXlab platform.
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Spore formation and karyological characterization of basidiosporogenesis, meiosis, post-meiotic and nuclear migration mitosis in Coprinus comatus
Mycoscience, 2020Co-Authors: Fu-chia Chen, Norihiro Shimomura, Takeshi Yamaguchi, Tadanori AimiAbstract:Abstract In Coprinus comatus, we sampled and observed different maturity stages of Basidium to find the possible causes of binucleate basidiospores by microscopy using the HCI-Giemsa and DAPI staining methods. In Basidium, following karyogamy, a single nucleolus was observed. We found evidence suggesting post-meiotic mitosis following nuclear migration in the spores. Post-meiotic mitosis occurred in the basidiospores, resulting in four binucleate basidiospores. This indicates that the basidiospore is a monokaryon, and that C. comatus shows the same pattern of basidiospore formation as Coprinopsis cinerea. This type of nuclear behavior was defined as pattern D, one of six distinct patterns (pattern A–F) of nuclear behavior during basidiosporogenesis have been described for basidiomycetes by Campos and Costa in 2010.
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karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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Karyological characterization of meiosis, post-meiotic mitosis and nuclear migration in the ectomycorrhizal fungus Rhizopogon roseolus (= R. rubescens)
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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short title rhizopogon roseolus karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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ultrastructure of developing basidiospores in rhizopogon roseolus r rubescens
Mycoscience, 2008Co-Authors: Norihiro Shimomura, Tadanori Aimi, Teruyuki Matsumoto, Nitaro Maekawa, Hiroshi OtaniAbstract:The ultrastructure of developing basidiospores in Rhizopogon roseolus is described. When viewed in the fruiting body chamber using scanning electron microscopy, basidiospores appear narrowly ellipsoid and have smooth walls. Eight basidiospores are usually produced on the apex of each sterigma on the Basidium. Transmission electron micrographs showed that basidiospores formed by movement of cytoplasm (including the nuclei) via the sterigmata, and then each basidiospore eventually became separated from its sterigma by an electron-lucent septum. The sterigma and Basidium subsequently collapsed, resulting in spore release. Freshly released spores retained the sterigmal appendage connected to the collapsed Basidium. After spore release, the major ultrastructural changes in the spore concerned the lipid bodies and the spore wall. During maturation, lipid bodies formed and then expanded. Before release, the spore wall was homogeneous and electronlucent, but after release the spore wall comprised two distinct layers with electron-dense depositions at the inner wall, and the dense depositions formed an electron-dense third layer. The mature spore wall complex comprised at least four distinct layers: the outer electron-lucent thin double layers, the mottled electron-dense third layer, and the electron-lucent fourth layer in which electron-lucent granular substances were dispersed.
Nitaro Maekawa - One of the best experts on this subject based on the ideXlab platform.
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karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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Karyological characterization of meiosis, post-meiotic mitosis and nuclear migration in the ectomycorrhizal fungus Rhizopogon roseolus (= R. rubescens)
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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short title rhizopogon roseolus karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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ultrastructure of developing basidiospores in rhizopogon roseolus r rubescens
Mycoscience, 2008Co-Authors: Norihiro Shimomura, Tadanori Aimi, Teruyuki Matsumoto, Nitaro Maekawa, Hiroshi OtaniAbstract:The ultrastructure of developing basidiospores in Rhizopogon roseolus is described. When viewed in the fruiting body chamber using scanning electron microscopy, basidiospores appear narrowly ellipsoid and have smooth walls. Eight basidiospores are usually produced on the apex of each sterigma on the Basidium. Transmission electron micrographs showed that basidiospores formed by movement of cytoplasm (including the nuclei) via the sterigmata, and then each basidiospore eventually became separated from its sterigma by an electron-lucent septum. The sterigma and Basidium subsequently collapsed, resulting in spore release. Freshly released spores retained the sterigmal appendage connected to the collapsed Basidium. After spore release, the major ultrastructural changes in the spore concerned the lipid bodies and the spore wall. During maturation, lipid bodies formed and then expanded. Before release, the spore wall was homogeneous and electronlucent, but after release the spore wall comprised two distinct layers with electron-dense depositions at the inner wall, and the dense depositions formed an electron-dense third layer. The mature spore wall complex comprised at least four distinct layers: the outer electron-lucent thin double layers, the mottled electron-dense third layer, and the electron-lucent fourth layer in which electron-lucent granular substances were dispersed.
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Ultrastructure of developing basidiospores in Rhizopogon roseolus (= R. rubescens)
Mycoscience, 2008Co-Authors: Norihiro Shimomura, Tadanori Aimi, Teruyuki Matsumoto, Nitaro Maekawa, Hiroshi OtaniAbstract:The ultrastructure of developing basidiospores in Rhizopogon roseolus is described. When viewed in the fruiting body chamber using scanning electron microscopy, basidiospores appear narrowly ellipsoid and have smooth walls. Eight basidiospores are usually produced on the apex of each sterigma on the Basidium. Transmission electron micrographs showed that basidiospores formed by movement of cytoplasm (including the nuclei) via the sterigmata, and then each basidiospore eventually became separated from its sterigma by an electron-lucent septum. The sterigma and Basidium subsequently collapsed, resulting in spore release. Freshly released spores retained the sterigmal appendage connected to the collapsed Basidium. After spore release, the major ultrastructural changes in the spore concerned the lipid bodies and the spore wall. During maturation, lipid bodies formed and then expanded. Before release, the spore wall was homogeneous and electronlucent, but after release the spore wall comprised two distinct layers with electron-dense depositions at the inner wall, and the dense depositions formed an electron-dense third layer. The mature spore wall complex comprised at least four distinct layers: the outer electron-lucent thin double layers, the mottled electron-dense third layer, and the electron-lucent fourth layer in which electron-lucent granular substances were dispersed.
Tadanori Aimi - One of the best experts on this subject based on the ideXlab platform.
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Spore formation and karyological characterization of basidiosporogenesis, meiosis, post-meiotic and nuclear migration mitosis in Coprinus comatus
Mycoscience, 2020Co-Authors: Fu-chia Chen, Norihiro Shimomura, Takeshi Yamaguchi, Tadanori AimiAbstract:Abstract In Coprinus comatus, we sampled and observed different maturity stages of Basidium to find the possible causes of binucleate basidiospores by microscopy using the HCI-Giemsa and DAPI staining methods. In Basidium, following karyogamy, a single nucleolus was observed. We found evidence suggesting post-meiotic mitosis following nuclear migration in the spores. Post-meiotic mitosis occurred in the basidiospores, resulting in four binucleate basidiospores. This indicates that the basidiospore is a monokaryon, and that C. comatus shows the same pattern of basidiospore formation as Coprinopsis cinerea. This type of nuclear behavior was defined as pattern D, one of six distinct patterns (pattern A–F) of nuclear behavior during basidiosporogenesis have been described for basidiomycetes by Campos and Costa in 2010.
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karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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Karyological characterization of meiosis, post-meiotic mitosis and nuclear migration in the ectomycorrhizal fungus Rhizopogon roseolus (= R. rubescens)
Mycologia, 2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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short title rhizopogon roseolus karyological characterization of meiosis post meiotic mitosis and nuclear migration in the ectomycorrhizal fungus rhizopogon roseolus r rubescens
2012Co-Authors: Norihiro Shimomura, Kiyomi Sawada, Tadanori Aimi, Nitaro Maekawa, Teruyuki MatsumotoAbstract:Karyological characteristics during basidiosporogenesis of Rhizopogon roseolus, a member of the hypogeous Agaricomycetes, were studied by light and transmission electron microscopy. More than 1000 tissue fragments of young basidiomata were stained with HCl-Giemsa and observed by a light microscopy to evaluate nuclear behavior. Basidium morphology in the hymenium was observed by transmission electron microscopy. Meiosis and post-meiotic mitosis took place in the center of the Basidium. Sterigmata appeared when the first meiotic division occurred, and the center of the Basidium became constricted when the second meiotic division occurred. Asynchronous nuclear migration from the Basidium into the basidiospores occurred after post-meiotic mitosis, producing eight uninucleate basidiospores. The nucleus migrated patchily into basidiospores. The pattern of post-meiotic mitosis of R. roseolus, in which post-meiotic mitosis took place in the center of the Basidium, is reported for the first time.
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ultrastructure of developing basidiospores in rhizopogon roseolus r rubescens
Mycoscience, 2008Co-Authors: Norihiro Shimomura, Tadanori Aimi, Teruyuki Matsumoto, Nitaro Maekawa, Hiroshi OtaniAbstract:The ultrastructure of developing basidiospores in Rhizopogon roseolus is described. When viewed in the fruiting body chamber using scanning electron microscopy, basidiospores appear narrowly ellipsoid and have smooth walls. Eight basidiospores are usually produced on the apex of each sterigma on the Basidium. Transmission electron micrographs showed that basidiospores formed by movement of cytoplasm (including the nuclei) via the sterigmata, and then each basidiospore eventually became separated from its sterigma by an electron-lucent septum. The sterigma and Basidium subsequently collapsed, resulting in spore release. Freshly released spores retained the sterigmal appendage connected to the collapsed Basidium. After spore release, the major ultrastructural changes in the spore concerned the lipid bodies and the spore wall. During maturation, lipid bodies formed and then expanded. Before release, the spore wall was homogeneous and electronlucent, but after release the spore wall comprised two distinct layers with electron-dense depositions at the inner wall, and the dense depositions formed an electron-dense third layer. The mature spore wall complex comprised at least four distinct layers: the outer electron-lucent thin double layers, the mottled electron-dense third layer, and the electron-lucent fourth layer in which electron-lucent granular substances were dispersed.