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M H Boskabadi - One of the best experts on this subject based on the ideXlab platform.
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Effects of hydro-ethanolic extract of Berberis vulgaris fruit on rabbit isolated heart
DARU, 2006Co-Authors: H Parsaee, M N Shafiei, M H BoskabadiAbstract:Several therapeutic effects including antimicrobial, antidiarrhea, hepatoprotection and cardiotonic for Berberis vulgaris have been described. In the present study, the effects of hydro-ethanolic extract of Berberis vulgaris on the rate and contractility of isolated heart were examined. The heart mounted on a modified Langendorff apparatus and circulation was perfused through aorta. Heart rate and contractility were determined in the presence of four concentrations of hydro-ethanolic extract (0.5, 1.0, 2.0 and 5.0 mg/100ml) and diltiazem, a calcium channel blocker (0.1, 1,10 and 100 µM) in comparison with baseline values in two different groups of experiments as follows: 1) Perfused heart with normal Krebs solution (group1 experiments, n=10). 2) Perfused heart with calcium free Krebs solution (group 2 experiments, n=9). In group 1 only 3 highest concentrations of diltiazem showed significant reduction in heart rate (p
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effects of hydro ethanolic extract of Berberis vulgaris fruit on rabbit isolated heart
DARU, 2006Co-Authors: H Parsaee, M N Shafiei, M H BoskabadiAbstract:Several therapeutic effects including antimicrobial, antidiarrhea, hepatoprotection and cardiotonic for Berberis vulgaris have been described. In the present study, the effects of hydro-ethanolic extract of Berberis vulgaris on the rate and contractility of isolated heart were examined. The heart mounted on a modified Langendorff apparatus and circulation was perfused through aorta. Heart rate and contractility were determined in the presence of four concentrations of hydro-ethanolic extract (0.5, 1.0, 2.0 and 5.0 mg/100ml) and diltiazem, a calcium channel blocker (0.1, 1,10 and 100 µM) in comparison with baseline values in two different groups of experiments as follows: 1) Perfused heart with normal Krebs solution (group1 experiments, n=10). 2) Perfused heart with calcium free Krebs solution (group 2 experiments, n=9). In group 1 only 3 highest concentrations of diltiazem showed significant reduction in heart rate (p<0.05 to P<0.001). However, 3 highest concentrations of diltiazem showed significant decrease and the last 2 concentrations of hydro-ethanolic extract increased heart contractility significantly (p<0.01 to P<0.001). In group 2 only the last concentration of diltiazem showed significant reduction in heart rate and contractility (p<0.05). The relationship between concentrations of hydro-ethanolic extract and heart rate in both group were negative (p<0.01 to p<0.001). However, there was positive correlation between concentrations of hydro-ethanolic extract and heart contractility. These results showed that of hydro-ethanolic extract of Berberis vulgaris has strong effect on heart contractility. The results of the present study may also indicate an activatoion of the calcium channel of isolated heart by the extract.
H Parsaee - One of the best experts on this subject based on the ideXlab platform.
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Effects of hydro-ethanolic extract of Berberis vulgaris fruit on rabbit isolated heart
DARU, 2006Co-Authors: H Parsaee, M N Shafiei, M H BoskabadiAbstract:Several therapeutic effects including antimicrobial, antidiarrhea, hepatoprotection and cardiotonic for Berberis vulgaris have been described. In the present study, the effects of hydro-ethanolic extract of Berberis vulgaris on the rate and contractility of isolated heart were examined. The heart mounted on a modified Langendorff apparatus and circulation was perfused through aorta. Heart rate and contractility were determined in the presence of four concentrations of hydro-ethanolic extract (0.5, 1.0, 2.0 and 5.0 mg/100ml) and diltiazem, a calcium channel blocker (0.1, 1,10 and 100 µM) in comparison with baseline values in two different groups of experiments as follows: 1) Perfused heart with normal Krebs solution (group1 experiments, n=10). 2) Perfused heart with calcium free Krebs solution (group 2 experiments, n=9). In group 1 only 3 highest concentrations of diltiazem showed significant reduction in heart rate (p
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effects of hydro ethanolic extract of Berberis vulgaris fruit on rabbit isolated heart
DARU, 2006Co-Authors: H Parsaee, M N Shafiei, M H BoskabadiAbstract:Several therapeutic effects including antimicrobial, antidiarrhea, hepatoprotection and cardiotonic for Berberis vulgaris have been described. In the present study, the effects of hydro-ethanolic extract of Berberis vulgaris on the rate and contractility of isolated heart were examined. The heart mounted on a modified Langendorff apparatus and circulation was perfused through aorta. Heart rate and contractility were determined in the presence of four concentrations of hydro-ethanolic extract (0.5, 1.0, 2.0 and 5.0 mg/100ml) and diltiazem, a calcium channel blocker (0.1, 1,10 and 100 µM) in comparison with baseline values in two different groups of experiments as follows: 1) Perfused heart with normal Krebs solution (group1 experiments, n=10). 2) Perfused heart with calcium free Krebs solution (group 2 experiments, n=9). In group 1 only 3 highest concentrations of diltiazem showed significant reduction in heart rate (p<0.05 to P<0.001). However, 3 highest concentrations of diltiazem showed significant decrease and the last 2 concentrations of hydro-ethanolic extract increased heart contractility significantly (p<0.01 to P<0.001). In group 2 only the last concentration of diltiazem showed significant reduction in heart rate and contractility (p<0.05). The relationship between concentrations of hydro-ethanolic extract and heart rate in both group were negative (p<0.01 to p<0.001). However, there was positive correlation between concentrations of hydro-ethanolic extract and heart contractility. These results showed that of hydro-ethanolic extract of Berberis vulgaris has strong effect on heart contractility. The results of the present study may also indicate an activatoion of the calcium channel of isolated heart by the extract.
Anca Dinischiotu - One of the best experts on this subject based on the ideXlab platform.
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Hepatoprotective Effects of Berberis vulgaris L. Extract/β Cyclodextrin on Carbon Tetrachloride–Induced Acute Toxicity in Mice
International Journal of Molecular Sciences, 2012Co-Authors: Anca Hermenean, Cristina Popescu, Aurel Ardelean, Miruna Silvia Stan, Nicoleta Hadaruga, Ciprian-valentin Mihali, Marieta Costache, Anca DinischiotuAbstract:The present study investigated the capacity of formulated Berberis vulgaris extract/β-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/β-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies.
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hepatoprotective effects of Berberis vulgaris l extract β cyclodextrin on carbon tetrachloride induced acute toxicity in mice
International Journal of Molecular Sciences, 2012Co-Authors: Anca Hermenean, Cristina Popescu, Aurel Ardelean, Miruna Silvia Stan, Nicoleta Hadaruga, Ciprian-valentin Mihali, Marieta Costache, Anca DinischiotuAbstract:The present study investigated the capacity of formulated Berberis vulgaris extract/β-cyclodextrin to protect liver against CCl4-induced hepatotoxicity in mice. Formulated and non-formulated extracts were given orally (50 mg/kg/day) to mice for 7 days and were then intra-peritoneally injected with 1.0 mL/kg CCl4 on the 8th day. After 24 h of CCl4 administration, an increase in the levels of apartate-amino-transferase (AST), alanine-amino-transferase (ALT) and malondialdehyde (MDA) was found and a significant decrease in superoxide-dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione-peroxidase (GPx) levels could be detected. This was accompanied by extended centrilobular necrosis, steatosis, fibrosis and an altered ultrastructure of hepatocytes. Pre-treatment with formulated or non-formulated extract suppressed the increase in ALT, AST and MDA levels and restored the level of antioxidant enzymes at normal values. Histopathological and electron-microscopic examination showed milder liver damage in both pre-treated groups and the protective effect was more pronounced after the formulated extract was administered. Internucleosomal DNA fragmentation induced by CCl4 was reduced in the group which received non-formulated extract and absent in the group which received formulated extract. Taken together, our results suggest that Berberis vulgaris/β-cyclodextrin treatment prevents hepatic injury induced by CCl4 and can be considered for further nutraceutical studies.
Z. Boucherit-otmani - One of the best experts on this subject based on the ideXlab platform.
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Évaluation de l’activité antioxydante de Berberis vulgaris L.
Phytothérapie, 2014Co-Authors: D. Mezouar, F.b. Lahfa, R. Djaziri, Z. Boucherit-otmaniAbstract:Natural substances from plants have multiple interests exploited in biotechnology industry both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites that are illustrated in many areas and even in therapy. In this context, we investigated the antioxidant activity of Berberis vulgaris , a plant used in Algeria, and it is particularly used in the Tlemcen region. After preparation of extracts from the root bark of Berberis vulgaris , total alkaloids were analyzed by thin layer chromatography, indicating the presence of six tasks. A quantitative analysis of polyphenols and flavonoids in methanolic extract was carried out, showing a content of 10.48 mg GAE/g of total polyphenols and 2.05 mg CEQ/g of flavonoids. The antioxidant activity of hydromethanolic extracts under reflux and total alkaloids was evaluated using two methods: reducing power and DPPH free radical scavenging. The results showed a high activity of methanolic extract than the total alkaloids, with an IC_50 of 1.40 mg/ml. Les substances naturelles issues des végétaux présentent des intérêts multiples mis à profit dans la biotechnologie tant dans l’industrie alimentaire, cosmétique que pharmaceutique. Parmi ces composés, on retrouve une grande partie des métabolites secondaires qui se sont illustrés dans beaucoup de domaines et même en thérapie. Dans ce contexte, nous avons recherché l’activité antioxydante de Berberis vulgaris , une plante utilisée en Algérie et plus particulièrement dans la région de Tlemcen. Après préparation des extraits à partir des écorces de racines de Berberis vulgaris , les alcaloïdes totaux ont été analysés par chromatographie sur couche mince, révélant la présence de six taches. Aussi, une analyse quantitative des polyphénols et des flavonoïdes de l’extrait hydrométhanolique a été réalisée, montrant une teneur de 10,48 mg GAE/g en polyphénols totaux et 2,05 mg CEQ/g en flavonoïdes. L’activité antioxydante des extraits hydrométhanolique et alcaloïdes totaux a été évaluée par deux méthodes: la réduction du fer et le piégeage du radical libre DPPH. Les résultats obtenus ont montré une activité élevée de l’extrait hydrométhanolique par rapport aux alcaloïdes totaux, avec une CI_50 de 1,40 mg/ml.
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Évaluation de l’activité antioxydante de Berberis vulgaris L.
Sozial-und Praventivmedizin, 2014Co-Authors: D. Mezouar, Farid Lahfa, R. Djaziri, Z. Boucherit-otmaniAbstract:Les substances naturelles issues des vegetaux presentent des interets multiples mis a profit dans la biotechnologie tant dans l’industrie alimentaire, cosmetique que pharmaceutique. Parmi ces composes, on retrouve une grande partie des metabolites secondaires qui se sont illustres dans beaucoup de domaines et meme en therapie. Dans ce contexte, nous avons recherche l’activite antioxydante de Berberis vulgaris, une plante utilisee en Algerie et plus particulierement dans la region de Tlemcen. Apres preparation des extraits a partir des ecorces de racines de Berberis vulgaris, les alcaloides totaux ont ete analyses par chromatographie sur couche mince, revelant la presence de six taches. Aussi, une analyse quantitative des polyphenols et des flavonoides de l’extrait hydromethanolique a ete realisee, montrant une teneur de 10,48 mg GAE/g en polyphenols totaux et 2,05 mg CEQ/g en flavonoides. L’activite antioxydante des extraits hydromethanolique et alcaloides totaux a ete evaluee par deux methodes: la reduction du fer et le piegeage du radical libre DPPH. Les resultats obtenus ont montre une activite elevee de l’extrait hydromethanolique par rapport aux alcaloides totaux, avec une CI50 de 1,40 mg/ml.
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Antimicrobial activity of Berberis vulgaris root bark extracts
Sozial-und Praventivmedizin, 2014Co-Authors: D. Mezouar, Farid Lahfa, D. E. Abdelouahid, H. Adida, N. M. Rahmoun, Z. Boucherit-otmaniAbstract:Natural substances from plants have multiple interests exploited in the biotechnology industries both in food, cosmetic and pharmaceutical. These compounds include a large proportion of secondary metabolites which are illustrated in many areas and even in therapy. We investigated the antimicrobial effects of Berberis vulgaris, a plant used in Algeria, and particularly in Tlemcen region. The qualitative phytochemical examination performed on Berberis vulgaris root bark showed the presence of alkaloids, tannins, sterols, triterpenes and reducing compounds in large quantities, and it also revealed lower amounts of coumarins, terpenoids, saponins and mucilage. The detection of these chemical classes is followed by extraction using solvents with increasing polarity and extraction of total alkaloids. Then, the different extracts were tested for their antimicrobial potency on different reference strains of bacteria and yeasts, using the agar diffusion technique and the determination of minimum inhibitory concentration MIC. The methanolic macerated extract had the best antibacterial activity with inhibition diameters from 12.5 to 21 mm for some strains. Also, extracts of Berberis vulgaris and more specifically alkaloids showed a strong antifungal activity on Candida albicans yeasts (diameters inhibition 19 to 29 mm).
Hossein Hosseinzadeh - One of the best experts on this subject based on the ideXlab platform.
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Berberine and barberry (Berberis vulgaris): A clinical review.
Phytotherapy Research, 2019Co-Authors: Mohsen Imenshahidi, Hossein HosseinzadehAbstract:Barberry (Berberis vulgaris L.) has different medicinal applications in folk medicine of Iran. Berberine, an alkaloid constituent of this plant, is present in the roots, rhizomes, stem, and bark of B. vulgaris and many other plants. There have been many clinical trials conducted that suggested a wide range of therapeutic applications. Here, we investigated the clinical uses of berberine and B. vulgaris in the treatment of different diseases in humans. An extensive search in electronic databases (PubMed, Scopus, Embase, Web of Sciences and Science Direct) was used to identify the clinical trials on B. vulgaris and berberine. Lipid-lowering and insulin-resistance improving actions are the most studied properties of berberine in numerous randomized clinical trials. There are also clinical trials regarding cardiovascular, anticancer, gastrointestinal, CNS, endocrine, and so on. Berberine has very low toxicity in usual doses and reveals clinical benefits without major side effects. Only mild gastrointestinal reactions may occur in some patients. The purpose of this review is to provide a summary concerning the clinical trials conducted on berberine to improve the clinical application of this nutraceutical in different diseases. In this review article, we used 77 clinical studies on human subjects.
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Berberis vulgaris and its constituent berberine as antidotes and protective agents against natural or chemical toxicities.
Iranian Journal of Basic Medical Sciences, 2017Co-Authors: Nooshin Mohammadzadeh, Soghra Mehri, Hossein HosseinzadehAbstract:Berberis vulgaris L (B. vulgaris) and its main constituent berberine have been used in traditional medicine for a long time. This medicinal plant and berberine have many properties that have attracted the attention of researchers over the time. According to several studies, B. vulgaris and berberine exhibited anti-inflammatory, antioxidant, anticonvulsant, antidepressant, anti-Alzheimer, anti-cancer, anti-arrhythmic, antiviral, antibacterial and anti-diabetic effects in both in vitro and in vivo experiments. In regard to many reports on protective effects of B. vulgaris and berberine on natural and chemical toxins, in the current review article, the inhibitory effects of these compounds against natural, industrial, environmental and chemical toxicities with focus on cellular mechanism have been categorized. It has been mentioned that berberine could ameliorate toxicity of chemical toxins in brain, heart, kidney, liver and lung in part through antioxidant, anti-inflammatory, anti-apoptotic, modulation of mitogen-activated protein kinase (MAPK) and nuclear factor-ĸB (NF-ĸB) signaling pathways.
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Toxicology effects of Berberis vulgaris (barberry) and its active constituent, berberine: a review
Iranian Journal of Basic Medical Sciences, 2017Co-Authors: Seyede Zohre Kamrani Rad, Maryam Rameshrad, Hossein HosseinzadehAbstract:Berberis vulgaris and berberine, its main component, traditionally have been used for treatment of various disorders. The pharmacological properties of them have been investigated using different in vivo and in vitro models. In spite of beneficial effects of B. vulgaris on different cell lines, there are documents have revealed negative impacts of it on animal and human. In this regards, the determination of its toxicity in a scientific view is necessary. In current report, we provide classified information about the toxicity of B. vulgaris and berberine in different conditions consist of acute, sub-acute, sub-chronic and chronic state. Besides, it discusses the cytotoxicity, genotoxicity, mutagenicity, and carcinogenicity of B. vulgaris and berberine as well as developmental toxicity and clinical studies. Data from the present study indicate that their toxicity is depending on the route and duration of administration. According to present study, they could induce GI upset and ulceration, immunotoxicity, phototoxicity, neurotoxicity, cardiotoxicity and jaundice in a dose dependent manner. They should be used with caution in pregnancy, neonatal and G6PD deficiency. Besides, consideration should be taken in co-administration of berberine with drugs that are metabolized with CYP enzymes due their inhibitory effects on these enzymes. Furthermore, they evoke cytotoxicity on both normal and cancer cell line which is time and concentration dependent.
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Berberis vulgaris and Berberine: An Update Review
Phytotherapy Research, 2016Co-Authors: Mohsen Imenshahidi, Hossein HosseinzadehAbstract:Berberine is an isoquinoline alkaloid present in several plants, including Coptis sp. and Berberis sp. Berberine is a customary component in Chinese medicine, and is characterized by a diversity of pharmacological effects. An extensive search in electronic databases (PubMed, Scopus, Ovid, Wiley, ProQuest, ISI, and Science Direct) were used to identify the pharmacological and clinical studies on Berberis vulgaris and berberine, during 2008 to 2015, using 'berberine' and 'Berberis vulgaris' as search words. We found more than 1200 new article studying the properties and clinical uses of berberine and B. vulgaris, for treating tumor, diabetes, cardiovascular disease, hyperlipidemia, inflammation, bacterial and viral infections, cerebral ischemia trauma, mental disease, Alzheimer disease, osteoporosis, and so on. In this article, we have updated the pharmacological effects of B. vulgaris and its active constituent, berberine. Copyright © 2016 John Wiley & Sons, Ltd.
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Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine.
Phytotherapy Research, 2008Co-Authors: Mohsen Imanshahidi, Hossein HosseinzadehAbstract:Barberry (Berberis vulgaris L. family Berberidaceae) is well known in Iran and various parts of this plant including its root, bark, leaf and fruit have been used as folk medicine. The two decades of research has demonstrated different pharmacological and therapeutic effects of B. vulgaris and its isoquinoline alkaloids (particularly berberine). Studies carried out on the chemical composition of the plant show that the most important constituents of this plant are isoquinoline alkaloids such as berberine, berbamine and palmatine. Berberine represents one of the most studied among the naturally occurring protoberberine alkaloids. In addition to B. vulgaris (barberry), berberine is present in many other plants and is used for the treatment of different diseases. This article reviews the traditional uses and pharmacological effects of total extract and the most active ingredient of B. vulgaris (berberine).
