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Daniel M.p. Ardisson-araújo - One of the best experts on this subject based on the ideXlab platform.

  • The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog
    Virology, 2019
    Co-Authors: Luana Beló Trentin, Bergmann Morais Ribeiro, Ethiane Rozo Dos Santos, Admilton G. Oliveira Junior, Daniel R. Sosa-gómez, Daniel M.p. Ardisson-araújo
    Abstract:

    Abstract We described a novel baculovirus isolated from the polyphagous insect pest Rachiplusia nu. The virus presented pyramidal-shaped occlusion bodies (OBs) with singly-embed nucleocapsids and a dose mortality response of 6.9 × 103 OBs/ml to third-instar larvae of R. nu. The virus genome is 128,587 bp long with a G + C content of 37.9% and 134 predicted ORFs. The virus is an alphabaculovirus closely related to Trichoplusia ni single nucleopolyhedrovirus, Chrysodeixis chalcites nucleopolyhedrovirus, and Chrysodeixis includens single nucleopolyhedrovirus and may constitute a new species. Surprisingly, we found co-evolution among the related viruses and their hosts at species level. Besides, auxiliary genes with homologs in other baculoviruses were found, e.g. a CPD-photolyase. The gene seemed to be result of a single event of horizontal transfer from lepidopterans to alphabaculovirus, followed by a transference from alpha to Betabaculovirus. The predicted protein appears to be an active enzyme that ensures likely DNA protection from sunlight.

  • Genome-wide diversity in temporal and regional populations of the Betabaculovirus Erinnyis ello granulovirus (ErelGV)
    BMC Genomics, 2018
    Co-Authors: A. F. Brito, Daniel M.p. Ardisson-araújo, W. Sihler, F. L. Melo, M. L. Souza, B. M. Ribeiro
    Abstract:

    Background Erinnyis ello granulovirus (ErelGV) is a Betabaculovirus infecting caterpillars of the sphingid moth E. ello ello (cassava hornworm), an important pest of cassava crops ( Manihot esculenta ). In this study, the genome of seven field isolates of the virus ErelGV were deep sequenced and their inter- and intrapopulational sequence diversity were analyzed. Results No events of gene gain/loss or translocations were observed, and indels were mainly found within highly repetitive regions (direct repeats, drs ). A naturally occurring isolate from Northern Brazil (Acre State, an Amazonian region) has shown to be the most diverse population, with a unique pattern of polymorphisms. Overall, non-synonymous substitutions were found all over the seven genomes, with no specific gathering of mutations on hotspot regions. Independently of their sizes, some ORFs have shown higher levels of non-synonymous changes than others. Non-core genes of known functions and structural genes were among the most diverse ones; and as expected, core genes were the least variable genes. We observed remarkable differences on diversity of paralogous genes, as in multiple copies of p10 , fgf , and pep . Another important contrast on sequence diversity was found on genes encoding complex subunits and/or involved in the same biological processes, as late expression factors ( lefs ) and per os infectivity factors ( pifs ). Interestingly, several polymorphisms in coding regions lie on sequences encoding specific protein domains. Conclusions By comparing and integrating information about inter- and intrapopulational diversity of viral isolates, we provide a detailed description on how evolution operates on field isolates of a Betabaculovirus. Our results revealed that 35–41% of the SNPs of ErelGV lead to amino acid changes (non-synonymous substitutions). Some genes, especially non-core genes of unknown functions, tend to accumulate more mutations, while core genes evolve slowly and are more conserved. Additional studies would be necessary to understand the actual effects of such gene variations on viral infection and fitness.

  • Genome-wide diversity in temporal and regional populations of the Betabaculovirus Erinnyis ello granulovirus (ErelGV)
    2018
    Co-Authors: A. F. Brito, Marlinda Lobo De Souza, Daniel M.p. Ardisson-araújo, Fernando L. Melo, W. Sihler, Bergmann Morais Ribeiro
    Abstract:

    Erinnyis ello granulovirus (ErelGV) is a Betabaculovirus infecting caterpillars of the sphingid moth E. ello ello (cassava hornworm), an important pest of cassava crops (Manihot esculenta). In this study, the genome of seven field isolates of the virus ErelGV were deep sequenced and their inter- and intrapopulational sequence diversity were analyzed. No events of gene gain/loss or translocations were observed, and indels were mainly found within highly repetitive regions (direct repeats, drs). A naturally occurring isolate from Northern Brazil (Acre State, an Amazonian region) has shown to be the most diverse population, with a unique pattern of polymorphisms. Overall, non-synonymous substitutions were found all over the seven genomes, with no specific gathering of mutations on hotspot regions. Independently of their sizes, some ORFs have shown higher levels of non-synonymous changes than others. Non-core genes of known functions and structural genes were among the most diverse ones; and as expected, core genes were the least variable genes. We observed remarkable differences on diversity of paralogous genes, as in multiple copies of p10, fgf, and pep. Another important contrast on sequence diversity was found on genes encoding complex subunits and/or involved in the same biological processes, as late expression factors (lefs) and per os infectivity factors (pifs). Interestingly, several polymorphisms in coding regions lie on sequences encoding specific protein domains. By comparing and integrating information about inter- and intrapopulational diversity of viral isolates, we provide a detailed description on how evolution operates on field isolates of a Betabaculovirus. Our results revealed that 35-41% of the SNPs of ErelGV lead to amino acid changes (non-synonymous substitutions). Some genes, especially non-core genes of unknown functions, tend to accumulate more mutations, while core genes evolve slowly and are more conserved. Additional studies would be necessary to understand the actual effects of such gene variations on viral infection and fitness.

  • A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera: Noctuidae) and the Evolution of Multiple-Copy Genes
    MDPI AG, 2018
    Co-Authors: Daniel M.p. Ardisson-araújo, Ethiane Rozo Dos Santos, Daniel R. Sosa-gómez, Fernando L. Melo, Ana Maria Rodrigues Da Silva, Bergmann M. Ribeiro
    Abstract:

    In this report, we described the genome of a novel baculovirus isolated from the monocot insect pest Mocis latipes, the striped grass looper. The genome has 134,272 bp in length with a G + C content of 38.3%. Based on the concatenated sequence of the 38 baculovirus core genes, we found that the virus is a Betabaculovirus closely related to the noctuid-infecting Betabaculoviruses including Pseudaletia unipuncta granulovirus (PsunGV), Trichoplusia ni granulovirus (TnGV), Helicoverpa armigera granulovirus (HearGV), and Xestia c-nigrum granulovirus (XecnGV). The virus may constitute a new Betabaculovirus species tentatively named Mocis latipes granulovirus (MolaGV). After gene content analysis, five open reading frames (ORFs) were found to be unique to MolaGV and several auxiliary genes were found including iap-3, iap-5, bro-a, bro-b, and three enhancins. The virus genome lacked both chitinase and cathepsin. We then looked at the evolutionary history of the enhancin gene and found that Betabaculovirus acquired this gene from an alphabaculovirus followed by several duplication events. Gene duplication also happened to an endonuclease-like gene. Genomic and gene content analyses revealed both a strict collinearity and gene expansion into the genome of the MolaGV-related species. We also characterized the granulin gene using a recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and found that occlusion bodies were produced into the nucleus of infected cells and presented a polyhedral shape and no occluded virions within. Overall, Betabaculovirus genome sequencing is of importance to the field as few genomes are publicly accessible. Mocis latipes is a secondary pest of maize, rice, and wheat crops in Brazil. Certainly, both the discovery and description of novel baculoviruses may lead to development of greener and safer pesticides in order to counteract and effectively control crop damage-causing insect population

  • A Betabaculovirus encoding a gp64 homolog
    BMC Genomics, 2016
    Co-Authors: Daniel M.p. Ardisson-araújo, Daniel R. Sosa-gómez, Fernando L. Melo, Bergmann M. Ribeiro, Elliot W. Kitajima, Bruna T. Pereira, Paolo M. De A. Zanotto, Flávio Moscardi, José L. C. Wolff
    Abstract:

    Background A Betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. Results The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 Betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister group to a cluster formed by ChocGV, ErelGV, PiraGV isolates, ClanGV, CaLGV, CpGV, CrleGV, AdorGV, PhopGV and EpapGV. Surprisingly, we found in the DisaGV genome a G protein-coupled receptor related to lepidopteran and other insect virus genes and a gp64 homolog, which is likely a product of horizontal gene transfer from Group 1 alphabaculoviruses. Conclusion DisaGV represents a distinct lineage of the genus Betabaculovirus . It is closely related to the CpGV-related group and presents the smallest genome in size so far. Remarkably, we found a homolog of gp64 , which was reported solely in group 1 alphabaculovirus genomes so far.

Bergmann Morais Ribeiro - One of the best experts on this subject based on the ideXlab platform.

  • a novel cypovirus found in a Betabaculovirus co infection context contains a poxvirus immune nuclease poxin related gene
    Journal of General Virology, 2020
    Co-Authors: Leonardo Assis Da Silva, Daniel M P Ardissonaraujo, Marlinda Lobo De Souza, Brenda R. De Camargo, Bergmann Morais Ribeiro
    Abstract:

    The cassava hornworm Erinnyis ello ello (Lepidoptera: Sphingidae) is an important pest in Brazil. This insect feeds on host plants of several species, especially Manihot esculenta (cassava) and Hevia brasiliensis (rubber tree). Cassava hornworm outbreaks are quite common in Brazil and can cause great impact over crop production. Granulare and polyhedral-shaped occlusion bodies (OBs) were observed in extracts of dead E. ello larvae from rubber-tree plantations by light and scanning electron microscopy (SEM), suggesting a mixed infection. The polyhedral-shaped OB surface revealed indentations that resemble those found in cypovirus polyhedra. After OB nucleic acid extraction followed by cDNA production and Illumina deep-sequencing analysis, the results confirmed for the presence of a putative novel cypovirus that carries ten segments and also a Betabaculovirus (Erinnyis ello granulovirus, ErelGV). Phylogenetic analysis of the predicted segment 1-enconded RdRP showed that the new cypovirus isolate is closely related to a member of species Cypovirus 2, which was isolated from Inachis io (Lepidoptera: Nymphalidae). Therefore, we named this new isolate Erinnyis ello cypovirus 2 (ErelCPV-2). Genome in silico analyses showed that ErelCPV-2 segment 8 (S8) has a predicted amino acid identity of 35.82 % to a hypothetical protein of Betabaculoviruses. This putative protein has a cGAMP-specific nuclease domain related to the poxvirus immune nucleases (poxins) from the 2′,3′-cGAMP-degrading enzyme family.

  • The complete genome of Rachiplusia nu nucleopolyhedrovirus (RanuNPV) and the identification of a baculoviral CPD-photolyase homolog
    Virology, 2019
    Co-Authors: Luana Beló Trentin, Bergmann Morais Ribeiro, Ethiane Rozo Dos Santos, Admilton G. Oliveira Junior, Daniel R. Sosa-gómez, Daniel M.p. Ardisson-araújo
    Abstract:

    Abstract We described a novel baculovirus isolated from the polyphagous insect pest Rachiplusia nu. The virus presented pyramidal-shaped occlusion bodies (OBs) with singly-embed nucleocapsids and a dose mortality response of 6.9 × 103 OBs/ml to third-instar larvae of R. nu. The virus genome is 128,587 bp long with a G + C content of 37.9% and 134 predicted ORFs. The virus is an alphabaculovirus closely related to Trichoplusia ni single nucleopolyhedrovirus, Chrysodeixis chalcites nucleopolyhedrovirus, and Chrysodeixis includens single nucleopolyhedrovirus and may constitute a new species. Surprisingly, we found co-evolution among the related viruses and their hosts at species level. Besides, auxiliary genes with homologs in other baculoviruses were found, e.g. a CPD-photolyase. The gene seemed to be result of a single event of horizontal transfer from lepidopterans to alphabaculovirus, followed by a transference from alpha to Betabaculovirus. The predicted protein appears to be an active enzyme that ensures likely DNA protection from sunlight.

  • Genome-wide diversity in temporal and regional populations of the Betabaculovirus Erinnyis ello granulovirus (ErelGV)
    2018
    Co-Authors: A. F. Brito, Marlinda Lobo De Souza, Daniel M.p. Ardisson-araújo, Fernando L. Melo, W. Sihler, Bergmann Morais Ribeiro
    Abstract:

    Erinnyis ello granulovirus (ErelGV) is a Betabaculovirus infecting caterpillars of the sphingid moth E. ello ello (cassava hornworm), an important pest of cassava crops (Manihot esculenta). In this study, the genome of seven field isolates of the virus ErelGV were deep sequenced and their inter- and intrapopulational sequence diversity were analyzed. No events of gene gain/loss or translocations were observed, and indels were mainly found within highly repetitive regions (direct repeats, drs). A naturally occurring isolate from Northern Brazil (Acre State, an Amazonian region) has shown to be the most diverse population, with a unique pattern of polymorphisms. Overall, non-synonymous substitutions were found all over the seven genomes, with no specific gathering of mutations on hotspot regions. Independently of their sizes, some ORFs have shown higher levels of non-synonymous changes than others. Non-core genes of known functions and structural genes were among the most diverse ones; and as expected, core genes were the least variable genes. We observed remarkable differences on diversity of paralogous genes, as in multiple copies of p10, fgf, and pep. Another important contrast on sequence diversity was found on genes encoding complex subunits and/or involved in the same biological processes, as late expression factors (lefs) and per os infectivity factors (pifs). Interestingly, several polymorphisms in coding regions lie on sequences encoding specific protein domains. By comparing and integrating information about inter- and intrapopulational diversity of viral isolates, we provide a detailed description on how evolution operates on field isolates of a Betabaculovirus. Our results revealed that 35-41% of the SNPs of ErelGV lead to amino acid changes (non-synonymous substitutions). Some genes, especially non-core genes of unknown functions, tend to accumulate more mutations, while core genes evolve slowly and are more conserved. Additional studies would be necessary to understand the actual effects of such gene variations on viral infection and fitness.

  • A Betabaculovirus encoding a gp64 homolog.
    BMC genomics, 2016
    Co-Authors: Daniel M.p. Ardisson-araújo, Paolo Marinho De Andrade Zanotto, Bergmann Morais Ribeiro, Daniel R. Sosa-gómez, Fernando L. Melo, Sônia Nair Báo, Elliot W. Kitajima, Bruna T. Pereira, Flávio Moscardi, José L. C. Wolff
    Abstract:

    A Betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil. The complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 Betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister group to a cluster formed by ChocGV, ErelGV, PiraGV isolates, ClanGV, CaLGV, CpGV, CrleGV, AdorGV, PhopGV and EpapGV. Surprisingly, we found in the DisaGV genome a G protein-coupled receptor related to lepidopteran and other insect virus genes and a gp64 homolog, which is likely a product of horizontal gene transfer from Group 1 alphabaculoviruses. DisaGV represents a distinct lineage of the genus Betabaculovirus. It is closely related to the CpGV-related group and presents the smallest genome in size so far. Remarkably, we found a homolog of gp64, which was reported solely in group 1 alphabaculovirus genomes so far.

  • A Betabaculovirus-Encoded gp64 Homolog Codes for a Functional Envelope Fusion Protein
    Journal of virology, 2015
    Co-Authors: Daniel M.p. Ardisson-araújo, Fernando L. Melo, Rollie J. Clem, José Luiz Caldas Wolff, Bergmann Morais Ribeiro
    Abstract:

    The GP64 envelope fusion protein is a hallmark of group I alphabaculoviruses. However, the Diatraea saccharalis granulovirus genome sequence revealed the first Betabaculovirus species harboring a gp64 homolog (disa118). In this work, we have shown that this homolog encodes a functional envelope fusion protein and could enable the infection and fusogenic abilities of a gp64-null prototype baculovirus. Therefore, GP64 may complement or may be in the process of replacing F protein activity in this virus lineage.

Marlinda Lobo De Souza - One of the best experts on this subject based on the ideXlab platform.

  • a novel cypovirus found in a Betabaculovirus co infection context contains a poxvirus immune nuclease poxin related gene
    Journal of General Virology, 2020
    Co-Authors: Leonardo Assis Da Silva, Daniel M P Ardissonaraujo, Marlinda Lobo De Souza, Brenda R. De Camargo, Bergmann Morais Ribeiro
    Abstract:

    The cassava hornworm Erinnyis ello ello (Lepidoptera: Sphingidae) is an important pest in Brazil. This insect feeds on host plants of several species, especially Manihot esculenta (cassava) and Hevia brasiliensis (rubber tree). Cassava hornworm outbreaks are quite common in Brazil and can cause great impact over crop production. Granulare and polyhedral-shaped occlusion bodies (OBs) were observed in extracts of dead E. ello larvae from rubber-tree plantations by light and scanning electron microscopy (SEM), suggesting a mixed infection. The polyhedral-shaped OB surface revealed indentations that resemble those found in cypovirus polyhedra. After OB nucleic acid extraction followed by cDNA production and Illumina deep-sequencing analysis, the results confirmed for the presence of a putative novel cypovirus that carries ten segments and also a Betabaculovirus (Erinnyis ello granulovirus, ErelGV). Phylogenetic analysis of the predicted segment 1-enconded RdRP showed that the new cypovirus isolate is closely related to a member of species Cypovirus 2, which was isolated from Inachis io (Lepidoptera: Nymphalidae). Therefore, we named this new isolate Erinnyis ello cypovirus 2 (ErelCPV-2). Genome in silico analyses showed that ErelCPV-2 segment 8 (S8) has a predicted amino acid identity of 35.82 % to a hypothetical protein of Betabaculoviruses. This putative protein has a cGAMP-specific nuclease domain related to the poxvirus immune nucleases (poxins) from the 2′,3′-cGAMP-degrading enzyme family.

  • Genome-wide diversity in temporal and regional populations of the Betabaculovirus Erinnyis ello granulovirus (ErelGV)
    2018
    Co-Authors: A. F. Brito, Marlinda Lobo De Souza, Daniel M.p. Ardisson-araújo, Fernando L. Melo, W. Sihler, Bergmann Morais Ribeiro
    Abstract:

    Erinnyis ello granulovirus (ErelGV) is a Betabaculovirus infecting caterpillars of the sphingid moth E. ello ello (cassava hornworm), an important pest of cassava crops (Manihot esculenta). In this study, the genome of seven field isolates of the virus ErelGV were deep sequenced and their inter- and intrapopulational sequence diversity were analyzed. No events of gene gain/loss or translocations were observed, and indels were mainly found within highly repetitive regions (direct repeats, drs). A naturally occurring isolate from Northern Brazil (Acre State, an Amazonian region) has shown to be the most diverse population, with a unique pattern of polymorphisms. Overall, non-synonymous substitutions were found all over the seven genomes, with no specific gathering of mutations on hotspot regions. Independently of their sizes, some ORFs have shown higher levels of non-synonymous changes than others. Non-core genes of known functions and structural genes were among the most diverse ones; and as expected, core genes were the least variable genes. We observed remarkable differences on diversity of paralogous genes, as in multiple copies of p10, fgf, and pep. Another important contrast on sequence diversity was found on genes encoding complex subunits and/or involved in the same biological processes, as late expression factors (lefs) and per os infectivity factors (pifs). Interestingly, several polymorphisms in coding regions lie on sequences encoding specific protein domains. By comparing and integrating information about inter- and intrapopulational diversity of viral isolates, we provide a detailed description on how evolution operates on field isolates of a Betabaculovirus. Our results revealed that 35-41% of the SNPs of ErelGV lead to amino acid changes (non-synonymous substitutions). Some genes, especially non-core genes of unknown functions, tend to accumulate more mutations, while core genes evolve slowly and are more conserved. Additional studies would be necessary to understand the actual effects of such gene variations on viral infection and fitness.

  • Genome sequence of Erinnyis ello granulovirus (ErelGV), a natural cassava hornworm pesticide and the first sequenced sphingid-infecting Betabaculovirus
    BMC Genomics, 2014
    Co-Authors: Daniel M.p. Ardisson-araújo, Bergmann Morais Ribeiro, Fernando Lucas De Melo, Miguel De Souza Andrade, William Sihler, Marlinda Lobo De Souza
    Abstract:

    Background Cassava ( Manihot esculenta ) is the basic source for dietary energy of 500 million people in the world. In Brazil, Erinnyis ello ello (Lepidoptera: Sphingidae) is a major pest of cassava crops and a bottleneck for its production. In the 1980s, a naturally occurring baculovirus was isolated from E. ello larva and successfully applied as a bio-pesticide in the field. Here, we described the structure, the complete genome sequence, and the phylogenetic relationships of the first sphingid-infecting Betabaculovirus. Results The baculovirus isolated from the cassava hornworm cadavers is a Betabaculovirus designated Erinnyis ello granulovirus (ErelGV). The 102,759 bp long genome has a G + C content of 38.7%. We found 130 putative ORFs coding for polypeptides of at least 50 amino acid residues. Only eight genes were found to be unique. ErelGV is closely related to ChocGV and PiraGV isolates. We did not find typical homologous regions and cathepsin and chitinase homologous genes are lacked. The presence of he65 and p43 homologous genes suggests horizontal gene transfer from Alphabaculovirus . Moreover, we found a nucleotide metabolism-related gene and two genes that could be acquired probably from Densovirus . Conclusions The ErelGV represents a new virus species from the genus Betabaculovirus and is the closest relative of ChocGV. It contains a dUTPase -like, a he65 -like, p43 -like genes, which are also found in several other alpha- and Betabaculovirus genomes, and two Densovirus -related genes. Importantly, recombination events between insect viruses from unrelated families and genera might drive baculovirus genomic evolution.

  • Genome sequence of Erinnyis ello granulovirus (ErelGV), a natural cassava hornworm pesticide and the first sequenced sphingid-infecting Betabaculovirus
    BMC genomics, 2014
    Co-Authors: Daniel M.p. Ardisson-araújo, Bergmann Morais Ribeiro, Fernando L. Melo, Sônia Nair Báo, W. Sihler, Miguel De Souza Andrade, Marlinda Lobo De Souza
    Abstract:

    Background Cassava (Manihot esculenta) is the basic source for dietary energy of 500 million people in the world. In Brazil, Erinnyis ello ello (Lepidoptera: Sphingidae) is a major pest of cassava crops and a bottleneck for its production. In the 1980s, a naturally occurring baculovirus was isolated from E. ello larva and successfully applied as a bio-pesticide in the field. Here, we described the structure, the complete genome sequence, and the phylogenetic relationships of the first sphingid-infecting Betabaculovirus.

Laura Villamizar - One of the best experts on this subject based on the ideXlab platform.

  • Novel biopesticide based on Erinnyis ello Betabaculovirus: characterization and preliminary field evaluation to control Erinnyis ello in rubber plantations.
    Pest management science, 2018
    Co-Authors: Paola Cuartas, Gloria Barrera, Laura Villamizar, Jenny Carolina Ruiz, Juan C Campos, Guillermo León‐martínez, Juliana Gómez-valderrama
    Abstract:

    BACKGROUND The hornworn Erinnyis ello is the major pest of natural rubber crops in Colombia, mainly controlled using toxic chemical insecticides. The use of E. ello Betabaculovirus is an environmentally sustainable alternative for its control. The aim of the present work was to characterize a prototype biopesticide formulation and evaluate its efficacy under different conditions. RESULTS Quality control evaluations of formulated biopesticide revealed that all the parameters evaluated were under the permissible level. The lethal concentrations LC50 and LC90 of the biopesticide were 4.3 × 103 and 5.5 × 104 occlusion bodies (OBs) mL-1 , respectively. Biopesticide efficacies against second and fourth instar larvae under greenhouse conditions were higher than 80%. Evaluation of two application rates in a clonal garden resulted in 84% and 88% efficacy, comparable to that obtained with the chemical. The biopesticide in a commercial plantation showed efficacies between 74% and 82%. Biopesticide post-application persistence was estimated at least in 1 week under field natural conditions. Results allowed selection of the lowest evaluated dose (1 × 1011  OBs ha-1 ) as the basis for further field evaluations. CONCLUSION Formulated ErelGV showed high efficacy to control the hornworm in rubber crops and high potential to be included in integrated pest management programs, thus it could be an interesting alternative to replace agrochemicals. © 2018 Society of Chemical Industry.

  • The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus
    Viruses, 2015
    Co-Authors: Paola Cuartas, Mariano Nicolás Belaich, Pablo Daniel Ghiringhelli, Gloria Barrera, Emiliano Barreto, Laura Villamizar
    Abstract:

    Spodoptera frugiperda (Lepidoptera: Noctuidae) is a major pest in maize crops in Colombia, and affects several regions in America. A granulovirus isolated from S. frugiperda (SfGV VG008) has potential as an enhancer of insecticidal activity of previously described nucleopolyhedrovirus from the same insect species (SfMNPV). The SfGV VG008 genome was sequenced and analyzed showing circular double stranded DNA of 140,913 bp encoding 146 putative ORFs that include 37 Baculoviridae core genes, 88 shared with Betabaculoviruses, two shared only with Betabaculoviruses from Noctuide insects, two shared with alphabaculoviruses, three copies of own genes (paralogs) and the other 14 corresponding to unique genes without representation in the other baculovirus species. Particularly, the genome encodes for important virulence factors such as 4 chitinases and 2 enhancins. The sequence analysis revealed the existence of eight homologous regions (hrs) and also suggests processes of gene acquisition by horizontal transfer including the SfGV VG008 ORFs 046/047 (paralogs), 059, 089 and 099. The bioinformatics evidence indicates that the genome donors of mentioned genes could be alpha- and/or Betabaculovirus species. The previous reported ability of SfGV VG008 to naturally co-infect the same host with other virus show a possible mechanism to capture genes and thus improve its fitness.

  • Characterisation of a Colombian granulovirus (Baculoviridae: Betabaculovirus) isolated from Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae
    Biocontrol Science and Technology, 2014
    Co-Authors: Paola Cuartas, Gloria Barrera, Emiliano Barreto, Laura Villamizar
    Abstract:

    A strategy for biological control of the fall armyworm, Spodoptera frugiperda, has included the use of baculoviruses principally the nucleopolyhedrovirus SfMNPV, which have been extensively characterised. In contrast, the granulovirus of S. frugiperda (SfGV) has been poorly studied even though it is able to enhance the infectivity and virulence of NPVs. In this work, a Colombian SfGV isolate (VG008) was characterised in comparison with a reference isolate from Brazil (VG014). The viral morphology was characterised by ovoidal-shaped occlusion bodies (OB) that contained one single internal virion. Median lethal concentrations (LC50) and mean times to death (MTD) were 4.5 × 105 OBs/mL and 29 days for VG008 and 1.6 × 105 OBs/mL and 33 days for VG014. Both isolates reduced their insecticidal activity by 94%, after one hour of direct irradiation with ultraviolet light type B. The most prominent protein had an apparent molecular mass of 27 kDa and corresponded with the Granulin. Genomic comparison among isolates...

  • experimental mixtures of phthorimaea operculella granulovirus isolates provide high biological efficacy on both phthorimaea operculella and tecia solanivora lepidoptera gelechiidae
    Journal of Invertebrate Pathology, 2012
    Co-Authors: Carlos Espinelcorreal, Laura Villamizar, M Lopezferber, Jeanlouis Zeddam, Juliana Gomez, Alba Marina Cotes, Xavier Léry
    Abstract:

    Abstract The Guatemalan potato moth Tecia solanivora (Povolny) recently invaded part of South America, colonizing zones where Phthorimaea operculella (Zeller), another potato moth species belonging to the same group, was previously established. T. solanivora is now the major insect pest of potato in this area encompassing Venezuela, Colombia and Ecuador. P. operculella granulovirus (PhopGV) (Betabaculovirus) is a biocontrol agent to be considered for the simultaneous management of these two potato pests, instead of classical chemical insecticides. In a previous work, five PhopGV isolates were isolated in Colombia from T. solanivora and were tested against larvae of the same species showing variable efficacies. Infections with mixtures of different genotypes of Baculoviruses had been carried out in a wide range of species and several showed interesting results. In the present study, the effect of sequential passages of PhopGV in P. operculella and T. solanivora larvae was analyzed through biological assays. Three different mixtures containing a Peruvian PhopGV isolate (Peru) adapted to P. operculella and a Colombian PhopGV isolate (VG003) adapted to T. solanivora were tested. A preliminary analysis of the correlation between the genotypic marker egt gene and the level of pathogenicity after a variable number of replication cycles was made. Mixtures of virus isolates showed a higher efficacy in both hosts compared to individual PhopGV isolates. This higher pathogenicity was maintained through passages. In P. operculella the mixtures were between 2.8 and 23.6-fold (from 7.15 OB/mm2 to 0.10 OB/mm2) more pathogenic than isolate Peru applied alone. In T. solanivora they were between 2.3 and 4.9-fold (from 12.29 OB/mm2 to 1.25 OB/mm2) more pathogenic than isolate VG003 alone. Viral biopesticide containing a mixture of selected genotypes active against each hosts seemed suitable for the development of a biopesticide aimed to simultaneously control P. operculella and T. solanivora.

Johannes A. Jehle - One of the best experts on this subject based on the ideXlab platform.

  • Agrotis segetum nucleopolyhedrovirus but not Agrotis segetum granulovirus replicate in AiE1611T cell line of Agrotisipsilon.
    Journal of invertebrate pathology, 2017
    Co-Authors: Gianpiero Gueli Alletti, Eric B. Carstens, Birgit Weihrauch, Johannes A. Jehle
    Abstract:

    Abstract Both Agrotis segetum nucleopolyhedrovirus B (AgseNPV-B) and Agrotis segetum granulovirus (AgseGV) belong to a cluster of four baculoviruses that are infective for different Agrotis species. Belonging further to different baculovirus genera, namely Alphabaculovirus and Betabaculovirus, respectively, AgseNPV-B and AgseGV are candidates to investigate virus interactions in co-infections. However, for the investigation of virus interactions on a cellular level, permissive insect cell-lines are needed. The cell line AiE1611T deriving from Agrotis ipsilon eggs has been shown to be permissive for several Alphabaculovirus isolates. In this study, virus replication was followed based on microscopic analysis of infected and transfected cells, as well as on a molecular level by PCR of DNA and cDNA of selected baculovirus transcripts. While the permissivity was not verified for AgseGV, AgseNPV-B produced occlusion bodies in both infection with hemolymph of infected larvae and Lipofectamin transfection with AgseNPV-B genomic DNA. In addition to the possibility to investigate virus interaction of AgseNPV-B with other alphabaculoviruses, the permissivity of AiE1611T for AgseNPV-B further offers the possibility a biological selection to separate AgseNPV-B from AgseGV.

  • the genome sequence of agrotis segetum granulovirus isolate agsegv da reveals a new Betabaculovirus species of a slow killing granulovirus
    Journal of Invertebrate Pathology, 2017
    Co-Authors: Gianpiero Gueli Alletti, Eric B. Carstens, Marina Eigenbrod, Regina G Kleespies, Johannes A. Jehle
    Abstract:

    Abstract The European isolate Agrotis segetum granulovirus DA (AgseGV-DA) is a slow killing, type I granulovirus due to low dose-mortality responses within seven days post infection and a tissue tropism of infection restricted solely to the fat body of infected Agrotis segetum host larvae. The genome of AgseGV-DA was completely sequenced and compared to the whole genome sequences of the Chinese isolates AgseGV-XJ and AgseGV-L1. All three isolates share highly conserved genomes. The AgseGV-DA genome is 131,557 bp in length and encodes for 149 putative open reading frames, including 37 baculovirus core genes and the per os infectivity factor ac110. Comprehensive investigations of repeat regions identified one putative non-hr like origin of replication in AgseGV-DA. Phylogenetic analysis based on concatenated amino acid alignments of 37 baculovirus core genes as well as pairwise distances based on the nucleotide alignments of partial granulin, lef-8 and lef-9 sequences with deposited Betabaculoviruses confirmed AgseGV-DA, AgseGV-XJ and AgseGV-L1 as representative isolates of the same Betabaculovirus species. AgseGV encodes for a distinct putative enhancin, distantly related to enhancins from other granuloviruses.

  • Transformation Associated Recombination of an AgseNPV-B bacmid
    2014
    Co-Authors: Laurin R. Monnheimer, Gianpiero Gueli Alletti, Johannes A. Jehle
    Abstract:

    Four baculoviruses, namely Agrotis segetum nucleopolyhedrovirus A (AgseNPV-A), A. segetum (Agse) NPV-B, A.ipsilon (Agip) NPV and A. segetum granulovirus (AgseGV) from the genera Alphaand Betabaculovirus, respectively, are known to infect larvae of the lepidopteran pests A. segetum and A. ipsilon. All four baculoviruses have a potential for being used as biological control agent against Agrotis pests. Despite genomic sequence information, studies about the infection cycle on a molecular and cellular level are still in favor. These studies require bacmids, recombinant baculoviruses, for their genomic manipulation and successive functional studies of genes in larvae and cell culture assays. In the past Bacmid construction was performed either in insect cell culture or in recombination’s with E. coli. These methods were either time-consuming or lacked in the recombination quality of large and highly repetitive genomes like for E. coli.

  • The Agrotis baculovirus complex: multiple viruses for multiple pests
    2011
    Co-Authors: Jörg T. Wennmann, Gianpiero Gueli Alletti, Wael El-menofy, Waly Essam, Naglaa A. Abdallah, Johannes A. Jehle
    Abstract:

    Larvae of the genus Agrotis (Lepidoptera: Noctuidae) are known to be severe soil pests on a wide range of field crops and vegetables in Europe, Asia and Africa. Agrotis spec. are highly susceptible for a broad number of baculoviruses and in the past, two Alphabaculoviruses (AgseNPV-A (Polish strain), AgseNPV-B (Oxford strain)) and one Betabaculovirus (AgseGV) were isolated from the common cutworm A. segetum. From larvae of the black cutworm A. ipsilon another Alphabaculovirus, Agrotis ipsilon nucleopolyhedrovirus (AgipNPV, Illinois strain), was isolated. Bioassay analysis demonstrated the cross-infectivity of all four baculoviruses for both hosts, which made them potential biocontrol agents for the control of cutworms. Especially in terms of resistance management the usage of a combination of different baculoviruses is regarded to be useful. In order to develop methods for identification of the different viruses we developed a multiplex polymerase chain reaction (PCR) and quantitative PCR (qPCR) based method. The genome of AgseNPV-B was completely sequenced and a comparative genome analysis of AgseNPV-B, AgseNPV-A and AgipNPV was conducted. Phylogenetic analysis confirmed the close relationship of AgseNPV-B and AgipNPV by a high sequence similarity, although the genome length and number of open reading frames (ORF) of AgseNPV-B and AgseNPV-A were more alike. For biological characterization bioassays and the determination of the median lethal dose (LC50) of AgipNPV and AgseNPV for their common host A. segetum, were performed. This work is the basis to analyze the molecular and cellular interaction of these viruses in mixed infections and to optimize the application of these viruses for Agrotis