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Jingou Tong - One of the best experts on this subject based on the ideXlab platform.
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qtl fine mapping for sex determination region in Bighead Carp hypophthalmichthys nobilis and comparison with silver Carp hypophthalmichthys molitrix
Marine Biotechnology, 2020Co-Authors: Ying Zhou, Haiyang Liu, Xinhua Wang, Jingou TongAbstract:Bighead Carp (Hypophthalmichthys nobilis) and silver Carp (Hypophthalmichthys molitrix) are genetically close aquaculture fish in the Cyprinidae, which have been confirmed to hold XX/XY sex determination. However, genomic locations of potential sex-related loci in these two fishes are still unknown. In this study, a high-resolution genetic linkage map was constructed by using 2976 SNP and 924 microsatellite markers in a F1 full-sib family of Bighead Carp, the length of which spanned 2022.34 cM with an average inter-marker distance of 0.52 cM. Comparative genomics revealed a high level of genomic synteny between Bighead Carp and zebrafish as well as grass Carp. QTL fine mapping for sex trait was performed based on this linkage map of Bighead Carp and an unpublished linkage map of silver Carp. A map distance of 3.863 cM (69.787–73.650 cM) on LG19 of Bighead Carp and 4.705 cM (79.096–83.801 cM) on LG21 of silver Carp was significantly associated with sex phenotypes, and these two LGs are homologous between two fish species. Fourteen markers harboring in these regions were in strong linkage disequilibrium with the sex phenotype variance explained (PVE) varying from 89 to 100%. Two common markers were mapped on the QTL regions of Bighead Carp and silver Carp, suggesting that these two Carp species may have similar genetic bases for sex determination. Eleven potentially sex-related genes were identified within or near the sex QTL markers in two species. This study provided insights into elucidating mechanisms and evolution of sex determination in cyprinid fishes.
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molecular cloning expression pattern of follistatin gene and association analysis with growth traits in Bighead Carp hypophthalmichthys nobilis
Comparative Biochemistry and Physiology B, 2018Co-Authors: Meixia Pang, Jingou Tong, Ying ZhouAbstract:Abstract Follistatin (FST) is a single-chain gonadal protein involving in various biological effects. FST plays important roles in not only ovary development but also body growth, whereas myostatin (MSTN) negatively regulates muscle growth. In this study, FST gene in Bighead Carp (HynFST) was cloned and characterized. A 5797 bp genomic sequence of HynFST, consisting six exons and five introns were cloned. The full-length cDNA of HynFST (2134 bp) has an open reading fragment encoding a polypeptide of 349 amino acids. Sequence comparison and phylogenetic analysis confirmed that FSTs are conserved throughout the vertebrates and HynFST belongs to FST-1 isoform. Nine single nucleotide polymorphisms (SNPs) of the HynFST were identified and three of them (g.2443 T > C, g.2852 T > C and g.5483A > G) were significantly associated with four growth-related traits. The average body weight of those fish with the combined genotype (CC CC GG) was 12.15–22.63% higher than that of triplotype (TT TT AA) in two Bighead Carp populations. HynFST was expressed in most of the development stages and various tissues with highest level in ovary. The co-expression results for FST and MSTN in brain and muscle of divergent weight groups showed that FST may inhibit MSTN expression, thus enhancing growth in Bighead Carp. Our results suggest that FST has significant genetic effects on the regulation of early growth in Bighead Carp. This study would facilitate the elucidation of multiple functions of FST gene in fish and exploration of the potentials as a gene marker in selective breeding programs for growth of Bighead Carp.
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Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in Bighead Carp (Hypophthalmichehys nobilis) and silver Carp (Hypophthalmichthys molitrix).
DNA research : an international journal for rapid publication of reports on genes and genomes, 2018Co-Authors: Haiyang Liu, Meixia Pang, Ying Zhou, Jingou TongAbstract:Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead Carp (Hypophthalmichehys nobilis) and silver Carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in Bighead Carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in Bighead Carp and silver Carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in Bighead Carp and silver Carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large Carp species.
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comparative transcriptomic analyses of two Bighead Carp hypophthalmichthys nobilis groups with different growth rates
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2016Co-Authors: Xinhua Wang, Xiu Feng, Jingou TongAbstract:Growth is one of the most important and desired economic traits for aquaculture species, and identification of loci controlling growth is a difficult task without genomic sequences. In this study, the liver transcriptomes of two groups (F and S) of Bighead Carp (Hypophthalmichthys nobilis) within a full-sib family with significant differences in growth rate were sequenced. Following de novo assembly of the combined reads from the two groups, a total of 410 differentially expressed genes were identified. Functional annotation and analysis of these genes indicated that some of these were involved in regulation of glucose levels and lipid metabolism, particularly fatty acid oxidation and transport. In addition to the differences on expression levels between the two groups, we also identified many non-synonymous coding single-nucleotide polymorphisms (SNPs) that were specific to each group, including SNPs from 4 genes involved in the lipid metabolism process (GO: 0006629). These differences in gene expression and DNA sequences may in part comprise the genetic background for the regulation of early growth rate in Bighead Carp.
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a high density genetic map and growth related qtl mapping in Bighead Carp hypophthalmichthys nobilis
Scientific Reports, 2016Co-Authors: Haiyang Liu, Jingou TongAbstract:Growth related traits in fish are controlled by quantitative trait loci (QTL), but no QTL for growth have been detected in Bighead Carp (Hypophthalmichthys nobilis) due to the lack of high-density genetic map. In this study, an ultra-high density genetic map was constructed with 3,121 SNP markers by sequencing 117 individuals in a F1 family using 2b-RAD technology. The total length of the map was 2341.27 cM, with an average marker interval of 0.75 cM. A high level of genomic synteny between our map and zebrafish was detected. Based on this genetic map, one genome-wide significant and 37 suggestive QTL for five growth-related traits were identified in 6 linkage groups (i.e. LG3, LG11, LG15, LG18, LG19, LG22). The phenotypic variance explained (PVE) by these QTL varied from 15.4% to 38.2%. Marker within the significant QTL region was surrounded by CRP1 and CRP2, which played an important role in muscle cell division. These high-density map and QTL information provided a solid base for QTL fine mapping and comparative genomics in Bighead Carp.
Haiyang Liu - One of the best experts on this subject based on the ideXlab platform.
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qtl fine mapping for sex determination region in Bighead Carp hypophthalmichthys nobilis and comparison with silver Carp hypophthalmichthys molitrix
Marine Biotechnology, 2020Co-Authors: Ying Zhou, Haiyang Liu, Xinhua Wang, Jingou TongAbstract:Bighead Carp (Hypophthalmichthys nobilis) and silver Carp (Hypophthalmichthys molitrix) are genetically close aquaculture fish in the Cyprinidae, which have been confirmed to hold XX/XY sex determination. However, genomic locations of potential sex-related loci in these two fishes are still unknown. In this study, a high-resolution genetic linkage map was constructed by using 2976 SNP and 924 microsatellite markers in a F1 full-sib family of Bighead Carp, the length of which spanned 2022.34 cM with an average inter-marker distance of 0.52 cM. Comparative genomics revealed a high level of genomic synteny between Bighead Carp and zebrafish as well as grass Carp. QTL fine mapping for sex trait was performed based on this linkage map of Bighead Carp and an unpublished linkage map of silver Carp. A map distance of 3.863 cM (69.787–73.650 cM) on LG19 of Bighead Carp and 4.705 cM (79.096–83.801 cM) on LG21 of silver Carp was significantly associated with sex phenotypes, and these two LGs are homologous between two fish species. Fourteen markers harboring in these regions were in strong linkage disequilibrium with the sex phenotype variance explained (PVE) varying from 89 to 100%. Two common markers were mapped on the QTL regions of Bighead Carp and silver Carp, suggesting that these two Carp species may have similar genetic bases for sex determination. Eleven potentially sex-related genes were identified within or near the sex QTL markers in two species. This study provided insights into elucidating mechanisms and evolution of sex determination in cyprinid fishes.
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Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in Bighead Carp (Hypophthalmichehys nobilis) and silver Carp (Hypophthalmichthys molitrix).
DNA research : an international journal for rapid publication of reports on genes and genomes, 2018Co-Authors: Haiyang Liu, Meixia Pang, Ying Zhou, Jingou TongAbstract:Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead Carp (Hypophthalmichehys nobilis) and silver Carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in Bighead Carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in Bighead Carp and silver Carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in Bighead Carp and silver Carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large Carp species.
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a high density genetic map and growth related qtl mapping in Bighead Carp hypophthalmichthys nobilis
Scientific Reports, 2016Co-Authors: Haiyang Liu, Jingou TongAbstract:Growth related traits in fish are controlled by quantitative trait loci (QTL), but no QTL for growth have been detected in Bighead Carp (Hypophthalmichthys nobilis) due to the lack of high-density genetic map. In this study, an ultra-high density genetic map was constructed with 3,121 SNP markers by sequencing 117 individuals in a F1 family using 2b-RAD technology. The total length of the map was 2341.27 cM, with an average marker interval of 0.75 cM. A high level of genomic synteny between our map and zebrafish was detected. Based on this genetic map, one genome-wide significant and 37 suggestive QTL for five growth-related traits were identified in 6 linkage groups (i.e. LG3, LG11, LG15, LG18, LG19, LG22). The phenotypic variance explained (PVE) by these QTL varied from 15.4% to 38.2%. Marker within the significant QTL region was surrounded by CRP1 and CRP2, which played an important role in muscle cell division. These high-density map and QTL information provided a solid base for QTL fine mapping and comparative genomics in Bighead Carp.
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QTL fine mapping and identification of candidate genes for growth-related traits in Bighead Carp (Hypophthalmichehys nobilis)
Aquaculture, 2016Co-Authors: Haiyang Liu, Meixia Pang, Xiu Feng, Xinhua Wang, Jingou TongAbstract:Abstract Bighead Carp (Hypophthalmichthys nobilis) is a popular Asian aquaculture species, but its genetic improvement is still in infancy. Marker-assisted selection (MAS) can improve the selection efficiency of breeding programs in fish. In this study, we constructed a genetic linkage map in a F1 family of Bighead Carp using microsatellite markers. A total of 905 microsatellites were assigned onto 24 linkage groups (LGs) of a consensus map, which spanned 1631.7 cM of Bighead Carp genome with an average interval of 1.8 cM. Comparative genomics revealed a high level of genomic synteny between Bighead Carp and zebrafish. QTL mapping for growth traits was performed based on this linkage map, and three significant and 8 suggestive QTL associated with four growth traits (body length, body height, head length, body weight) were detected on LG9 and LG17, with 18.6–25.5% of phenotypic variance explained. Three candidate genes for growth were identified in or near the QTL intervals, and a SNP in one of the three genes, TP53BP2, was significantly associated with growth traits in different populations of Bighead Carp. These results of the high-density SSR genetic map and genome scan for QTL provide a basis for MAS and breeding programs for growth in Bighead Carp.
Xinhua Wang - One of the best experts on this subject based on the ideXlab platform.
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qtl fine mapping for sex determination region in Bighead Carp hypophthalmichthys nobilis and comparison with silver Carp hypophthalmichthys molitrix
Marine Biotechnology, 2020Co-Authors: Ying Zhou, Haiyang Liu, Xinhua Wang, Jingou TongAbstract:Bighead Carp (Hypophthalmichthys nobilis) and silver Carp (Hypophthalmichthys molitrix) are genetically close aquaculture fish in the Cyprinidae, which have been confirmed to hold XX/XY sex determination. However, genomic locations of potential sex-related loci in these two fishes are still unknown. In this study, a high-resolution genetic linkage map was constructed by using 2976 SNP and 924 microsatellite markers in a F1 full-sib family of Bighead Carp, the length of which spanned 2022.34 cM with an average inter-marker distance of 0.52 cM. Comparative genomics revealed a high level of genomic synteny between Bighead Carp and zebrafish as well as grass Carp. QTL fine mapping for sex trait was performed based on this linkage map of Bighead Carp and an unpublished linkage map of silver Carp. A map distance of 3.863 cM (69.787–73.650 cM) on LG19 of Bighead Carp and 4.705 cM (79.096–83.801 cM) on LG21 of silver Carp was significantly associated with sex phenotypes, and these two LGs are homologous between two fish species. Fourteen markers harboring in these regions were in strong linkage disequilibrium with the sex phenotype variance explained (PVE) varying from 89 to 100%. Two common markers were mapped on the QTL regions of Bighead Carp and silver Carp, suggesting that these two Carp species may have similar genetic bases for sex determination. Eleven potentially sex-related genes were identified within or near the sex QTL markers in two species. This study provided insights into elucidating mechanisms and evolution of sex determination in cyprinid fishes.
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comparative transcriptomic analyses of two Bighead Carp hypophthalmichthys nobilis groups with different growth rates
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2016Co-Authors: Xinhua Wang, Xiu Feng, Jingou TongAbstract:Growth is one of the most important and desired economic traits for aquaculture species, and identification of loci controlling growth is a difficult task without genomic sequences. In this study, the liver transcriptomes of two groups (F and S) of Bighead Carp (Hypophthalmichthys nobilis) within a full-sib family with significant differences in growth rate were sequenced. Following de novo assembly of the combined reads from the two groups, a total of 410 differentially expressed genes were identified. Functional annotation and analysis of these genes indicated that some of these were involved in regulation of glucose levels and lipid metabolism, particularly fatty acid oxidation and transport. In addition to the differences on expression levels between the two groups, we also identified many non-synonymous coding single-nucleotide polymorphisms (SNPs) that were specific to each group, including SNPs from 4 genes involved in the lipid metabolism process (GO: 0006629). These differences in gene expression and DNA sequences may in part comprise the genetic background for the regulation of early growth rate in Bighead Carp.
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QTL fine mapping and identification of candidate genes for growth-related traits in Bighead Carp (Hypophthalmichehys nobilis)
Aquaculture, 2016Co-Authors: Haiyang Liu, Meixia Pang, Xiu Feng, Xinhua Wang, Jingou TongAbstract:Abstract Bighead Carp (Hypophthalmichthys nobilis) is a popular Asian aquaculture species, but its genetic improvement is still in infancy. Marker-assisted selection (MAS) can improve the selection efficiency of breeding programs in fish. In this study, we constructed a genetic linkage map in a F1 family of Bighead Carp using microsatellite markers. A total of 905 microsatellites were assigned onto 24 linkage groups (LGs) of a consensus map, which spanned 1631.7 cM of Bighead Carp genome with an average interval of 1.8 cM. Comparative genomics revealed a high level of genomic synteny between Bighead Carp and zebrafish. QTL mapping for growth traits was performed based on this linkage map, and three significant and 8 suggestive QTL associated with four growth traits (body length, body height, head length, body weight) were detected on LG9 and LG17, with 18.6–25.5% of phenotypic variance explained. Three candidate genes for growth were identified in or near the QTL intervals, and a SNP in one of the three genes, TP53BP2, was significantly associated with growth traits in different populations of Bighead Carp. These results of the high-density SSR genetic map and genome scan for QTL provide a basis for MAS and breeding programs for growth in Bighead Carp.
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A second-generation genetic linkage map for Bighead Carp (Aristichthys nobilis) based on microsatellite markers.
Animal genetics, 2014Co-Authors: Chuankun Zhu, Jingou Tong, Xiu Feng, Xinhua Wang, Wei Guo, Huanzhang Liu, Yonghua Sun, Lusha LiuAbstract:Bighead Carp (Aristichthys nobilis) is an important aquaculture fish worldwide. Genetic linkage maps for the species were previously reported, but map resolution remained to be improved. In this study, a second-generation genetic linkage map was constructed for Bighead Carp through a pseudo-testcross strategy using interspecific hybrids between Bighead Carp and silver Carp. Of the 754 microsatellites genotyped in two interspecific mapping families (with 77 progenies for each family), 659 markers were assigned to 24 linkage groups, which were equal to the chromosome numbers of the haploid genome. The consensus map spanned 1917.3cM covering 92.8% of the estimated Bighead Carp genome with an average marker interval of 2.9cM. The length of linkage groups ranged from 52.2 to 133.5cM with an average of 79.9cM. The number of markers per linkage group varied from 11 to 55 with an average of 27.5 per linkage group. Normality tests on interval distances of the map showed a non-normal marker distribution; however, significant correlation was found between the length of linkage group and the number of markers below the 0.01 significance level (two-tailed). The length of the female map was 1.12 times that of the male map, and the average recombination ratio of female to male was 1.10:1. Visual inspection showed that distorted markers gathered in some linkage groups and in certain regions of the male and female maps. This well-defined genetic linkage map will provide a basic framework for further genome mapping of quantitative traits, comparative mapping and marker-assisted breeding in Bighead Carp.
Ying Zhou - One of the best experts on this subject based on the ideXlab platform.
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qtl fine mapping for sex determination region in Bighead Carp hypophthalmichthys nobilis and comparison with silver Carp hypophthalmichthys molitrix
Marine Biotechnology, 2020Co-Authors: Ying Zhou, Haiyang Liu, Xinhua Wang, Jingou TongAbstract:Bighead Carp (Hypophthalmichthys nobilis) and silver Carp (Hypophthalmichthys molitrix) are genetically close aquaculture fish in the Cyprinidae, which have been confirmed to hold XX/XY sex determination. However, genomic locations of potential sex-related loci in these two fishes are still unknown. In this study, a high-resolution genetic linkage map was constructed by using 2976 SNP and 924 microsatellite markers in a F1 full-sib family of Bighead Carp, the length of which spanned 2022.34 cM with an average inter-marker distance of 0.52 cM. Comparative genomics revealed a high level of genomic synteny between Bighead Carp and zebrafish as well as grass Carp. QTL fine mapping for sex trait was performed based on this linkage map of Bighead Carp and an unpublished linkage map of silver Carp. A map distance of 3.863 cM (69.787–73.650 cM) on LG19 of Bighead Carp and 4.705 cM (79.096–83.801 cM) on LG21 of silver Carp was significantly associated with sex phenotypes, and these two LGs are homologous between two fish species. Fourteen markers harboring in these regions were in strong linkage disequilibrium with the sex phenotype variance explained (PVE) varying from 89 to 100%. Two common markers were mapped on the QTL regions of Bighead Carp and silver Carp, suggesting that these two Carp species may have similar genetic bases for sex determination. Eleven potentially sex-related genes were identified within or near the sex QTL markers in two species. This study provided insights into elucidating mechanisms and evolution of sex determination in cyprinid fishes.
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molecular cloning expression pattern of follistatin gene and association analysis with growth traits in Bighead Carp hypophthalmichthys nobilis
Comparative Biochemistry and Physiology B, 2018Co-Authors: Meixia Pang, Jingou Tong, Ying ZhouAbstract:Abstract Follistatin (FST) is a single-chain gonadal protein involving in various biological effects. FST plays important roles in not only ovary development but also body growth, whereas myostatin (MSTN) negatively regulates muscle growth. In this study, FST gene in Bighead Carp (HynFST) was cloned and characterized. A 5797 bp genomic sequence of HynFST, consisting six exons and five introns were cloned. The full-length cDNA of HynFST (2134 bp) has an open reading fragment encoding a polypeptide of 349 amino acids. Sequence comparison and phylogenetic analysis confirmed that FSTs are conserved throughout the vertebrates and HynFST belongs to FST-1 isoform. Nine single nucleotide polymorphisms (SNPs) of the HynFST were identified and three of them (g.2443 T > C, g.2852 T > C and g.5483A > G) were significantly associated with four growth-related traits. The average body weight of those fish with the combined genotype (CC CC GG) was 12.15–22.63% higher than that of triplotype (TT TT AA) in two Bighead Carp populations. HynFST was expressed in most of the development stages and various tissues with highest level in ovary. The co-expression results for FST and MSTN in brain and muscle of divergent weight groups showed that FST may inhibit MSTN expression, thus enhancing growth in Bighead Carp. Our results suggest that FST has significant genetic effects on the regulation of early growth in Bighead Carp. This study would facilitate the elucidation of multiple functions of FST gene in fish and exploration of the potentials as a gene marker in selective breeding programs for growth of Bighead Carp.
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Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in Bighead Carp (Hypophthalmichehys nobilis) and silver Carp (Hypophthalmichthys molitrix).
DNA research : an international journal for rapid publication of reports on genes and genomes, 2018Co-Authors: Haiyang Liu, Meixia Pang, Ying Zhou, Jingou TongAbstract:Sex-specific markers are powerful tools for identifying sex-determination system in various animals. Bighead Carp (Hypophthalmichehys nobilis) and silver Carp (Hypophthalmichthys molitrix) are two of the most important edible fish in Asia, which have a long juvenility period that can lasts for 4-5 years. In this study, we found one sex-specific marker by next-generation sequencing together with bioinformatics analysis in Bighead Carp. The male-specific markers were used to perform molecular sexing in the progenies of artificial gynogenetic diploids and found all progenies (n = 160) were females. Meanwhile, around 1 : 1 sex ratio was observed in a total of 579 juvenile offspring from three other families. To further extend the male-specific region, we performed genome walking and got a male-specific sequence of 8,661 bp. Five pairs of primers were designed and could be used to efficiently distinguish males from females in Bighead Carp and silver Carp. The development of these male-specific markers and results of their molecular sexing in different populations provide strong evidence for a sex determination system of female homogametry or male heterogametry (XX/XY) in Bighead Carp and silver Carp. To the best of our knowledge, this is the first report of effective sex-specific markers in these two large Carp species.
Yongkang Luo - One of the best experts on this subject based on the ideXlab platform.
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characterization of the microbiota in lightly salted Bighead Carp aristichthys nobilis fillets stored at 4 c
Food Microbiology, 2017Co-Authors: Xiaochang Liu, Yuemei Zhang, Yongkang LuoAbstract:The microbiota of unsalted and salted (dry-cured with 2% salt) Bighead Carp (Aristichthys nobilis) fillets during storage at 4 °C were identified by 16S rRNA gene analysis. Eleven genera were present in the initial microbiota of Bighead Carp fillets, where Acinetobacter, Aeromonas and Kocuria were the dominant bacteria. As storage time progressed, the microbial composition of both unsalted and salted fillets became less diverse. Additionally, differences in microbiota were observed between these two treatments. For unsalted Bighead Carp fillets, Aeromonas became the dominant genus at the end of storage and Pseudomonas was found less commonly. For salted fillets, Pseudomonas was the only bacteria identified at the end of storage.
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Characterization of the microbiota in lightly salted Bighead Carp (Aristichthys nobilis) fillets stored at 4 °C
Food microbiology, 2016Co-Authors: Xiaochang Liu, Yuemei Zhang, Yongkang LuoAbstract:The microbiota of unsalted and salted (dry-cured with 2% salt) Bighead Carp (Aristichthys nobilis) fillets during storage at 4 °C were identified by 16S rRNA gene analysis. Eleven genera were present in the initial microbiota of Bighead Carp fillets, where Acinetobacter, Aeromonas and Kocuria were the dominant bacteria. As storage time progressed, the microbial composition of both unsalted and salted fillets became less diverse. Additionally, differences in microbiota were observed between these two treatments. For unsalted Bighead Carp fillets, Aeromonas became the dominant genus at the end of storage and Pseudomonas was found less commonly. For salted fillets, Pseudomonas was the only bacteria identified at the end of storage.
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Seasonal variations of fatty acid profile in different tissues of farmed Bighead Carp (Aristichthys nobilis).
Journal of food science and technology, 2013Co-Authors: Hui Hong, Hongbing Fan, Hang Wang, Yongkang Luo, Huixing ShenAbstract:Bighead Carp (Aristichthys nobilis) is one of the major farmed species of freshwater fish in China. Byproduct volume of Bighead Carp is significant at up to 60 % of whole fish weight. A better understanding of the nutritional composition is needed to optimize the use of these raw materials. The objective of this research was to characterize seasonal variations of fatty acid profile in different tissues (heads, bones, skin, scales, viscera, muscle and fins) of farmed Bighead Carp. The fatty acid composition of farmed Bighead Carp varied significantly with seasons and tissues. The highest lipid content was determined in viscera while the highest EPA and DHA composition were observed in muscle compared to the other tissues. Significantly higher ΣEPA+DHA (%) was recorded in all tissues in summer (June) when compared with those of the other three seasons (p < 0.05). The n-3/n-6 fatty acid ratios in summer ranged from 3.38 to 3.69, nearly three times the ratios of the other three seasons. The results indicated that farmed Bighead Carp caught in summer could better balance the n-3 PUFA needs of consumers. The byproducts of Bighead Carp can be utilized for the production of fish oil.