The Experts below are selected from a list of 159 Experts worldwide ranked by ideXlab platform

Rui L. Reis - One of the best experts on this subject based on the ideXlab platform.

  • immobilization of Bioactive Factor loaded liposomes on the surface of electrospun nanofibers targeting tissue engineering
    Biomaterials Science, 2014
    Co-Authors: Nelson Monteiro, Rui L. Reis, Albino Martins, Ricardo A Pires, Susana Faria, Nuno A Fonseca, Joao Nuno Moreira, Nuno M Neves
    Abstract:

    Electrospun nanofiber meshes (NFM), due to their morphology and fibrous structure, are extensively proposed as biomedical devices, for tissue engineering on scaffolds and also as drug delivery systems. Liposomes are nanoparticles prepared from a biologically derived material (phospholipid), which are already in clinical use as a drug release device. Liposomes may be combined with biomaterial scaffolds to promote a local and sustained delivery of loaded Bioactive agents. The main objective of the present study is to evaluate the efficacy of dexamethasone (Dex)-loaded liposomes immobilized on the surface of electrospun polycaprolactone (PCL) NFM for promoting the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). The in vitro release profile demonstrates a sustained release of Dex over 21 days, after an initial burst release over 12 h. Biological assays show that Dex-loaded liposomes immobilized on the surface of electrospun PCL NFMs do not exhibit any cytotoxic effect, being able to successfully promote the osteogenic differentiation of hBMSCs. We herein validate the concept of using liposomes immobilized on the surface of a nanostructured fibrous system to be used as an advanced cell carrier device with autonomous release of growth/differentiation Factors relevant for tissue engineering and regenerative medicine strategies.

  • Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery
    Tissue engineering. Part B Reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.

  • controlled release strategies for bone cartilage and osteochondral engineering part ii challenges on the evolution from single to multiple Bioactive Factor delivery
    Tissue Engineering Part B-reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.

Eben Alsberg - One of the best experts on this subject based on the ideXlab platform.

  • Spatial regulation of controlled Bioactive Factor delivery for bone tissue engineering
    Advanced Drug Delivery Reviews, 2015
    Co-Authors: Julia E. Samorezov, Eben Alsberg
    Abstract:

    Limitations of current treatment options for critical size bone defects create a significant clinical need for tissue engineered bone strategies. This review describes how control over the spatiotemporal delivery of growth Factors, nucleic acids, and drugs and small molecules may aid in recapitulating signals present in bone development and healing, regenerating interfaces of bone with other connective tissues, and enhancing vascularization of tissue engineered bone. State-of-the-art technologies used to create spatially controlled patterns of Bioactive Factors on the surfaces of materials, to build up 3D materials with patterns of signal presentation within their bulk, and to pattern Bioactive Factor delivery after scaffold fabrication are presented, highlighting their applications in bone tissue engineering. As these techniques improve in areas such as spatial resolution and speed of patterning, they will continue to grow in value as model systems for understanding cell responses to spatially regulated Bioactive Factor signal presentation in vitro, and as strategies to investigate the capacity of the defined spatial arrangement of these signals to drive bone regeneration in vivo.

  • Bioactive Factor delivery strategies from engineered polymer hydrogels for therapeutic medicine.
    Progress in polymer science, 2014
    Co-Authors: Minh Khanh Nguyen, Eben Alsberg
    Abstract:

    Polymer hydrogels have been widely explored as therapeutic delivery matrices because of their ability to present sustained, localized and controlled release of Bioactive Factors. Bioactive Factor delivery from injectable biopolymer hydrogels provides a versatile approach to treat a wide variety of diseases, to direct cell function and to enhance tissue regeneration. The innovative development and modification of both natural-(e.g., alginate (ALG), chitosan, hyaluronic acid (HA), gelatin, heparin (HEP), etc.) and synthetic-(e.g., polyesters, polyethyleneimine (PEI), etc.) based polymers has resulted in a variety of approaches to design drug delivery hydrogel systems from which loaded therapeutics are released. This review presents the state-of-the-art in a wide range of hydrogels that are formed though self-assembly of polymers and peptides, chemical crosslinking, ionic crosslinking and biomolecule recognition. Hydrogel design for Bioactive Factor delivery is the focus of the first section. The second section then thoroughly discusses release strategies of payloads from hydrogels for therapeutic medicine, such as physical incorporation, covalent tethering, affinity interactions, on demand release and/or use of hybrid polymer scaffolds, with an emphasis on the last 5 years.

  • photocrosslinked alginate hydrogels with tunable biodegradation rates and mechanical properties
    Biomaterials, 2009
    Co-Authors: Oju Jeon, Kamal H Bouhadir, Joseph M Mansour, Eben Alsberg
    Abstract:

    Photocrosslinked and biodegradable alginate hydrogels were engineered for biomedical applications. Photocrosslinkable alginate macromers were prepared by reacting sodium alginate and 2-aminoethyl methacrylate in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride and N-hydroxysuccinimide. Methacrylated alginates were photocrosslinked using ultraviolet light with 0.05% photoinitiator. The swelling behavior, elastic moduli, and degradation rates of photocrosslinked alginate hydrogels were quantified and could be controlled by varying the degree of alginate methacrylation. The methacrylated alginate macromer and photocrosslinked alginate hydrogels exhibited low cytotoxicity when cultured with primary bovine chondrocytes. In addition, chondrocytes encapsulated in these hydrogels remained viable and metabolically active as demonstrated by Live/Dead cell staining and MTS assay. These photocrosslinked alginate hydrogels, with tailorable mechanical properties and degradation rates, may find great utility as therapeutic materials in regenerative medicine and Bioactive Factor delivery.

Vítor E. Santo - One of the best experts on this subject based on the ideXlab platform.

  • Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery
    Tissue engineering. Part B Reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.

  • controlled release strategies for bone cartilage and osteochondral engineering part ii challenges on the evolution from single to multiple Bioactive Factor delivery
    Tissue Engineering Part B-reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.

Manuela E. Gomes - One of the best experts on this subject based on the ideXlab platform.

  • Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery
    Tissue engineering. Part B Reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.

  • controlled release strategies for bone cartilage and osteochondral engineering part ii challenges on the evolution from single to multiple Bioactive Factor delivery
    Tissue Engineering Part B-reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.

João F. Mano - One of the best experts on this subject based on the ideXlab platform.

  • Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery
    Tissue engineering. Part B Reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.

  • controlled release strategies for bone cartilage and osteochondral engineering part ii challenges on the evolution from single to multiple Bioactive Factor delivery
    Tissue Engineering Part B-reviews, 2013
    Co-Authors: Vítor E. Santo, Manuela E. Gomes, João F. Mano, Rui L. Reis
    Abstract:

    The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth Factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of Bioactive Factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth Factors and cytokines involved in each biological response, it is expected that an appropriate combination of Bioactive Factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple Bioactive Factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of Bioactive Factors. A comprehensive collection of studies focused on the delivery of Bioactive Factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth Factors.