The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Jongheon Shin - One of the best experts on this subject based on the ideXlab platform.
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Bioactive Lipids from the sponge spirastrella abata
ChemInform, 2012Co-Authors: Kyoung Hwa Jang, Yoonyeong Lee, Chung J Sim, Jongheon ShinAbstract:Three sphingosine 4-sulfates (I) and a lysophosphatidylglycerol (II) are isolated from the Korean sponge Spirastrella abata.
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Bioactive Lipids from the sponge spirastrella abata
Bioorganic & Medicinal Chemistry Letters, 2012Co-Authors: Kyoung Hwa Jang, Yoonyeong Lee, Chung J Sim, Jongheon ShinAbstract:Abstract Three sphingosine 4-sulfates ( 1 − 3 ) and a lysophosphatidylglycerol ( 4 ) were isolated from the Korean sponge Spirastrella abata . The structures of these compounds were determined based on the combined results of spectroscopic analyses. Based on the results of combined synthesis and comparison of specific rotation and circular dichroism, the absolute configurations of 1 − 3 were found to be enantiomeric to the previously isolated metabolites. The configurations of 4 were also partially determined by similar chemical and spectroscopic methods. The compounds exhibited significant cytotoxicity and weak antimicrobial activity ( 1 ), as well as weak-to-moderate inhibitory activity against isocitrate lyase and Na + /K + -ATPase. A structure–activity relationship was found for the sphingosine 4-sulfates.
Mauro Maccarrone - One of the best experts on this subject based on the ideXlab platform.
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Bioactive Lipids inflammation and chronic diseases
Advanced Drug Delivery Reviews, 2020Co-Authors: Alessandro Leuti, Domenico Fazio, Marina Fava, Alessandra Piccoli, Sergio Oddi, Mauro MaccarroneAbstract:Endogenous Bioactive Lipids are part of a complex network that modulates a plethora of cellular and molecular processes involved in health and disease, of which inflammation represents one of the most prominent examples. Inflammation serves as a well-conserved defence mechanism, triggered in the event of chemical, mechanical or microbial damage, that is meant to eradicate the source of damage and restore tissue function. However, excessive inflammatory signals, or impairment of pro-resolving/anti-inflammatory pathways leads to chronic inflammation, which is a hallmark of chronic pathologies. All main classes of endogenous Bioactive Lipids - namely eicosanoids, specialized pro-resolving lipid mediators, lysoglycerophopshoLipids and endocannabinoids - have been consistently involved in the chronic inflammation that characterises pathologies such as cancer, diabetes, atherosclerosis, asthma, as well as autoimmune and neurodegenerative disorders and inflammatory bowel diseases. This review gathers the current knowledge concerning the involvement of endogenous Bioactive Lipids in the pathogenic processes of chronic inflammatory pathologies.
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Bioactive Lipids aliamides differentially modulate inflammatory responses of distinct subsets of primary human t lymphocytes
The FASEB Journal, 2018Co-Authors: Valerio Chiurchiu, Alessandro Leuti, Reem Smoum, Raphael Mechoulam, Mauro MaccarroneAbstract:Autacoid local injury antagonist amides (ALIAmides) are a family of endogenous Bioactive acyl ethanolamides that include the renowned palmitoyl ethanolamide (PEA), oleoyl ethanolamide (OEA), and stearoyl ethanolamide (SEA), and that are involved in several biologic processes such as nociception, lipid metabolism, and inflammation. The role of ALIAmides in the control of inflammatory processes has recently gained much attention and prompted the use of these molecules or their analogs, and the pharmacologic manipulation of their endogenous levels, as plausible therapeutic strategies in the treatment of several chronic inflammatory conditions. Since chronic inflammation is mainly driven by cells of adaptive immunity, particularly T lymphocytes, we aimed at investigating whether such Bioactive Lipids could directly modulate T-cell responses. We found that OEA, PEA, and eicosatrienoyl ethanolamide (ETEA) could directly inhibit both T-cell responses by reducing their production of TNF-α and IFN-γ from CD8 T cells and TNF-α, IFN-γ and IL-17 from CD4 T cells. Furthermore, neither SEA nor docosatrienoyl ethanolamide (DTEA) could affect cytokine production from both T cell subsets. Interestingly, unlike OEA and ETEA, PEA was also able to enhance de novo generation of forkhead box P3 (FoxP3)-expressing regulatory T cells from CD4-naive T cells. Our findings show for the first time that specific ALIAmides can directly affect different T-cell subsets, and provide proof of their anti-inflammatory role in chronic inflammation, ultimately suggesting that these Bioactive Lipids could offer novel tools for the management of T-cell dependent chronic inflammatory diseases.-Chiurchiu, V., Leuti, A., Smoum, R., Mechoulam, R., Maccarrone, M. Bioactive Lipids ALIAmides differentially modulate inflammatory responses of distinct subsets of primary human T lymphocytes.
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Bioactive Lipids and chronic inflammation managing the fire within
Frontiers in Immunology, 2018Co-Authors: Valerio Chiurchiu, Alessandro Leuti, Mauro MaccarroneAbstract:Inflammation is an immune response that works as a contained fire that is pre-emptively sparked as a defensive process during infections or upon any kind of tissue insult, and that is spontaneously extinguished after elimination or termination of the damage. However, persistent and uncontrolled immune reactions act as a wildfire that promote chronic inflammation, unresolved tissue damage and, eventually, chronic diseases. A wide network of soluble mediators, among which endogenous Bioactive Lipids, governs all immune processes. They are secreted by basically all cells involved in inflammatory processes and constitute the crucial infrastructure that triggers, coordinates and confines inflammatory mechanisms. However, these molecules are also deeply involved in the detrimental transition from acute to chronic inflammation, be it for persistent or excessive action of pro-inflammatory Lipids or for the impairment of the functions carried out by resolving ones. As a matter of fact, Bioactive Lipids have been linked, to date, to several chronic diseases, including rheumatoid arthritis, atherosclerosis, diabetes, cancer, inflammatory bowel disease, systemic lupus erythematosus, and multiple sclerosis. This review summarizes current knowledge on the involvement of the main classes of endogenous Bioactive Lipids-namely classical eicosanoids, pro-resolving lipid mediators, lysoglycerophosphoLipids/sphingoLipids, and endocannabinoids-in the cellular and molecular mechanisms that lead to the pathogenesis of chronic disorders.
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Bioactive Lipids as modulators of immunity inflammation and emotions
Current Opinion in Pharmacology, 2016Co-Authors: Valerio Chiurchiu, Mauro MaccarroneAbstract:Lipids are not only constituents of cellular membranes but also key signaling mediators, thus acting as 'Bioactive Lipids'. Among the prominent roles exerted by Bioactive Lipids are immune regulation, inflammation and maintenance of homeostasis. Accumulated evidence indicates the existence of a bidirectional relationship between immune and nervous systems, whereby inflammatory mediators can directly modulate emotions that, in turn, can strongly influence immune responses, thus affecting health. This review summarizes current knowledge on the ability of several families of Bioactive Lipids to regulate immunity and inflammation (through pro-inflammatory or anti-inflammatory effects), as well as to control emotions and mood-related manifestations, advocating these substances as an attractive interface between 'mind' and 'body', and as a potential target to treat inflammatory/immune-mediated mood disorders.
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assay of nape pld activity
Methods of Molecular Biology, 2016Co-Authors: Filomena Fezza, Nicolina Mastrangelo, Mauro MaccarroneAbstract:N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of Bioactive Lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).
Kyoung Hwa Jang - One of the best experts on this subject based on the ideXlab platform.
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Bioactive Lipids from the sponge spirastrella abata
ChemInform, 2012Co-Authors: Kyoung Hwa Jang, Yoonyeong Lee, Chung J Sim, Jongheon ShinAbstract:Three sphingosine 4-sulfates (I) and a lysophosphatidylglycerol (II) are isolated from the Korean sponge Spirastrella abata.
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Bioactive Lipids from the sponge spirastrella abata
Bioorganic & Medicinal Chemistry Letters, 2012Co-Authors: Kyoung Hwa Jang, Yoonyeong Lee, Chung J Sim, Jongheon ShinAbstract:Abstract Three sphingosine 4-sulfates ( 1 − 3 ) and a lysophosphatidylglycerol ( 4 ) were isolated from the Korean sponge Spirastrella abata . The structures of these compounds were determined based on the combined results of spectroscopic analyses. Based on the results of combined synthesis and comparison of specific rotation and circular dichroism, the absolute configurations of 1 − 3 were found to be enantiomeric to the previously isolated metabolites. The configurations of 4 were also partially determined by similar chemical and spectroscopic methods. The compounds exhibited significant cytotoxicity and weak antimicrobial activity ( 1 ), as well as weak-to-moderate inhibitory activity against isocitrate lyase and Na + /K + -ATPase. A structure–activity relationship was found for the sphingosine 4-sulfates.
Lian-wang Guo - One of the best experts on this subject based on the ideXlab platform.
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mass spectrometric imaging reveals temporal and spatial dynamics of Bioactive Lipids in arteries undergoing restenosis
Journal of Proteome Research, 2019Co-Authors: Yatao Shi, Jillian Johnson, Bowen Wang, Bingming Chen, Gregory L. Fisher, Go Urabe, Xudong Shi, Craig K. Kent, Lian-wang GuoAbstract:Restenosis, or renarrowing of the arterial lumen, is a common recurrent disease following balloon angioplasty and stenting treatments for cardiovascular disease. A major technical barrier for deciphering restenotic mechanisms is the dynamic, spatial profiling of Bioactive Lipids in the arterial wall, especially in small animals. Here, applying matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), we conducted the first lipidomic study of temporal-spatial profiling in a small animal model of angioplasty-induced restenosis. Cross sections were collected 3, 7, and 14 days after balloon angioplasty of rat carotid arteries. MALDI-MSI analyses showed that diacylglycerols (DAGs), signaling Lipids associated with restenosis, and lysophosphatidylcholines (LysoPCs), whose function was uncharacterized in restenosis, dramatically increased at postangioplasty day 7 and day 14 in the neointimal layer of balloon-injured arteries compared to uninjured controls. In contrast, sphingomyelins (SMs) did not increase, but rather decreased at day 3, day 7, and day 14 in injured arteries versus the uninjured control arteries. These results revealed previously unexplored distinct temporal-spatial lipid dynamics in the restenotic arterial wall. Additionally, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem MS imaging for both molecular identification and imaging at high spatial resolution. These imaging modalities provide powerful tools for unraveling novel mechanisms of restenosis involving Lipids or small signaling molecules.
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Mass Spectrometric Imaging Reveals Temporal and Spatial Dynamics of Bioactive Lipids in Arteries Undergoing Restenosis
2019Co-Authors: Yatao Shi, Jillian Johnson, Bowen Wang, Bingming Chen, Gregory L. Fisher, Go Urabe, Xudong Shi, Craig K. Kent, Lian-wang GuoAbstract:Restenosis, or renarrowing of the arterial lumen, is a common recurrent disease following balloon angioplasty and stenting treatments for cardiovascular disease. A major technical barrier for deciphering restenotic mechanisms is the dynamic, spatial profiling of Bioactive Lipids in the arterial wall, especially in small animals. Here, applying matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI), we conducted the first lipidomic study of temporal-spatial profiling in a small animal model of angioplasty-induced restenosis. Cross sections were collected 3, 7, and 14 days after balloon angioplasty of rat carotid arteries. MALDI-MSI analyses showed that diacylglycerols (DAGs), signaling Lipids associated with restenosis, and lysophosphatidylcholines (LysoPCs), whose function was uncharacterized in restenosis, dramatically increased at postangioplasty day 7 and day 14 in the neointimal layer of balloon-injured arteries compared to uninjured controls. In contrast, sphingomyelins (SMs) did not increase, but rather decreased at day 3, day 7, and day 14 in injured arteries versus the uninjured control arteries. These results revealed previously unexplored distinct temporal-spatial lipid dynamics in the restenotic arterial wall. Additionally, we employed time-of-flight secondary ion mass spectrometry (TOF-SIMS) tandem MS imaging for both molecular identification and imaging at high spatial resolution. These imaging modalities provide powerful tools for unraveling novel mechanisms of restenosis involving Lipids or small signaling molecules
Oliver Peyruchaud - One of the best experts on this subject based on the ideXlab platform.
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Bioactive Lipids lysophosphatidic acid and sphingosine 1 phosphate mediate breast cancer cell biological functions through distinct mechanisms
Oncology Research, 2009Co-Authors: Ahmed Boucharaba, Benoit Guillet, Farid Menaa, Mohamed Hneino, Andre J Van Wijnen, Philippe Clezardin, Oliver PeyruchaudAbstract:Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are structurally related Bioactive Lipids with growth factor-like activities. LPA and S1P are naturally produced in vivo by blood platelets upon platelet aggregation and at least in vitro by fibroblasts, adipocytes, and multiple types of tumor cells. Breast cancer cells respond to LPA and S1P. However, their specific actions on breast cancer cell biological functions remain unclear. We therefore conducted an in vitro side-by-side study of these two Lipids on breast cancer cells. LPA mediates human breast cancer MDA-BO2 cell proliferation, migration, and invasion through activation of a G(alpha i)/ERK1/2-dependent signaling pathway, whereas activation of G(alpha i)/PI3K predominates upon S1P stimulation. In MDA-BO2 cells, LPA but not S1P activities were dependent on active type 1 insulin-like growth factor and epithelial growth factor receptors. LPA and S1P act directly on endothelial cells to induce angiogenesis. We demonstrate that LPA and S1P have indirect angiogenic properties as judged by induced secretion of angiogenic factors by breast cancer cells primed with these lysophosphoLipids. S1P, but not LPA, controlled the expression of VEGF-A by breast cancer cells, while LPA, but not S1P, controlled the expression of GM-CSF, Gro-alpha, MCP-1, and IL-6. According to the secretion of these paracrine osteoclastic factors, LPA, but not S1P, stimulates breast cancer cell-induced osteoclastogenesis. These findings suggest that, in vivo, LPA and S1P can coordinate their action on tumor and surrounding cells to induce breast cancer progression both at primary and bone metastatic sites.