The Experts below are selected from a list of 702 Experts worldwide ranked by ideXlab platform

Alain Bergel - One of the best experts on this subject based on the ideXlab platform.

  • industrially scalable surface treatments to enhance the current density output from graphite Bioanodes fueled by real domestic wastewater
    iScience, 2021
    Co-Authors: Emma Roubaud, Alain Bergel, Luc Etcheverry, Rémy Lacroix, Serge Da Silva, Régine Basséguy, Jerome Esvan, Benjamin Erable
    Abstract:

    Acid and electrochemical surface treatments of graphite electrode, used individually or in combination, significantly improved the microbial anode current production, by +17% to +56%, in well-regulated and duplicated electroanalytical experimental systems. Of all the consequences induced by surface treatments, the modifications of the surface nano-topography preferentially justify an improvement in the fixation of bacteria, and an increase of the specific surface area and the electrochemically accessible surface of graphite electrodes, which are at the origin of the higher performances of the Bioanodes supplied with domestic wastewater. The evolution of the chemical composition and the appearance of C-O, C=O, and O=C-O groups on the graphite surface created by combining acid and electrochemical treatments was prejudicial to the formation of efficient domestic-wastewater-oxidizing Bioanodes. The comparative discussion, focused on the positioning of the performances, shows the industrial interest of applying the surface treatment method to the world of bioelectrochemical systems.

  • Benchmarking of Industrial Synthetic Graphite Grades, Carbon Felt, and Carbon Cloth as Cost-Efficient Bioanode Materials for Domestic Wastewater Fed Microbial Electrolysis Cells
    Frontiers in Energy Research, 2019
    Co-Authors: Emma Roubaud, Alain Bergel, Luc Etcheverry, Rémy Lacroix, Serge Da Silva, Régine Basséguy, Benjamin Erable
    Abstract:

    Anode material selection is crucial when it comes to building up-scaled microbialelectrolysis cells (MEC), as it has a huge influence on the achievable current density and account for a large part of the MEC total investment cost. Graphite is a material that isperfectly suited to the creation of up-scaled Bioanodes as it is conductive, chemically stable, biocompatible, and relatively cheap but there are a very large number of commercially available grades of industrial graphite. In this study, five grades of industrial synthetic graphite (named G1–G5) were bench tested to select the most suitable gradefor future development of 3D bioanode for domestic wastewater (dWW) fed MEC application. The five grades of graphite have been selected with similar physico-chemicaland surface properties (electrical resistivity, surface roughness, and hydrophobicity) theoretically appropriate for EA biofilm development. Nevertheless, significant current density disparities where observed with the five graphite grades, which can certainlybe explained by the fabrication procedures of the respective material grades. With thegraphite grade giving the most efficient anodes (G3), an average steady state currentdensity of 2.3 A/m²was produced, outperforming the other grades by at least 15%.Even though all graphites had very close physico-chemical characteristics, the gradehad a clear significant influence on the current densities produced. G3 graphite was finally compared to carbon felt (CF) and carbon cloth (CC) both in terms of bio-electrochemicalcurrent production and bacterial communities colonizing electrodes. G3 Bioanodes outperformed CF and CC Bioanodes by 50% in term of steady state current density.Biofilms microbial population analysis showed that theGeobacterspecies was presentat 82% on G3 Bioanodes, 39% on CF Bioanodes, and 61% on CC Bioanodes when it was only present at 0.06% in the activated sludge used as inoculum. This significant difference in bacterial enrichment could come from the huge gap between materials resistivity, as graphite resistivity is 200-fold lower than CF and CC resistivities. The strongly hydrophilic surface of G3 graphite was also certainly beneficial for biofilm development compared tothe hydrophobic surfaces of CF and CC.

  • Separator electrode assembly (SEA) with 3-dimensional bioanode and removable air-cathode boosts microbial fuel cell performance
    Journal of Power Sources, 2017
    Co-Authors: Manon Oliot, Luc Etcheverry, Régine Basséguy, Annette Mosdale, Marie-line Délia-dupuy, Alain Bergel
    Abstract:

    Separator electrode assemblies (SEAs) were designed by associating a microbial anode with an air-cathode on each side of three different kinds of separator: plastic grid, J-cloth and baking paper. The SEA was designed to allow the air-cathode be removed and replaced without disturbing the bioanode. Power densities up to 6.4 W m−2 were produced by the Grid-SEAs (on average 5.9 ± 0.5 W m−2) while JCloth-SEAs and Paper-SEAs produced 4.8 ± 0.3 and 1.8 ± 0.1 W m−2, respectively. Power densities decreased with time mainly because of fast deterioration of the cathode kinetics. They always increased again when the air-cathodes were replaced by new ones; the Grid-SEAs were thus boosted above 4 W m−2 after 7 weeks of operation. The theoretical analysis of SEA functioning suggested that the high performance of the Grid-SEAs was due to the combination of several virtuous phenomena: the efficient pH balance thanks to free diffusion through the large-mesh grid, the likely mitigation of oxygen crossover thanks to the 3-dimensional structure of the bioanode and the possibility of overcoming cathode fouling by replacing it during MFC operation. Finally, the microbial community of all Bioanodes showed stringent selection of Proteiniphilum acetatigenes in proportion with the performance.

  • Increasing the temperature is a relevant strategy to form microbial anodes intended to work at room temperature
    Electrochimica Acta, 2017
    Co-Authors: Manon Oliot, Benjamin Erable, Marie-line De Solan, Alain Bergel
    Abstract:

    Reducing the time required for the formation of microbial anodes from environmental inocula is a great challenge. The possibility of reaching this objective by increasing the temperature during the bioanode preparation was investigated here. Microbial anodes were formed at 25 °C and 40 °C under controlled potential with successive acetate additions. At 25 °C, around 40 days were required to perform three acetate batches, which led to current density of 9.4 ± 2 A.m−2, while at 40 °C, 20 days were sufficient to complete three similar batches, leading to 22.9 ± 4.2 A.m−2. The Bioanodes formed at 40 °C revealed three redox systems and those formed at 25 °C only one. The temperature also impacted the biofilm structure, which was less compact at 40 °C. When the Bioanodes formed at 40 °C were switched to 25 °C, they produced current densities similar to those of Bioanodes formed at 25 °C; they recovered the single redox system that was developed by the Bioanodes formed at 25 °C and the difference in biofilm structures was mitigated. It is consequently fully appropriate to accelerate the formation of microbial anodes by increasing the temperatures to 40 °C even if they are finally intended to operate at room temperature.

  • Halotolerant Bioanodes: The applied potential modulates the electrochemical characteristics, the biofilm structure and the ratio of the two dominant genera.
    Bioelectrochemistry, 2016
    Co-Authors: Raphaël Rousseau, Wafa Achouak, Anaïs Bonnafous, Marie-line Délia, Jean-jacques Godon, Catherine Santaella, Alain Bergel
    Abstract:

    Abstract The development of economically-efficient microbial electrochemical technologies remains hindered by the low ionic conductivity of the culture media used as the electrolyte. To overcome this drawback, halotolerant Bioanodes were designed with salt marsh sediment used as the inoculum in electrolytes containing NaCl at 30 or 45 g/L (ionic conductivity 7.0 or 10.4 S·m− 1). The Bioanodes were formed at four different potentials − 0.4, − 0.2, 0.0 and 0.2 V/SCE to identify the effect on the electrochemical kinetic parameters, the biofilm structures and the composition of the microbial communities. The Bioanodes formed at − 0.4 V/SCE were largely dominated by Marinobacter spp. Voltammetry showed that they provided higher currents than the other Bioanodes in the range of low potentials, but the maximum currents were limited by the poor surface colonization. The Bioanodes formed at − 0.2, 0.0 and 0.2 V/SCE showed similar ratios of Marinobacter and Desulfuromonas spp. and higher values of the maximum current density. The combined analysis of kinetic parameters, biofilm structure and biofilm composition showed that Marinobacter spp., which ensured a higher electron transfer rate, were promising species for the design of halotolerant Bioanodes. The challenge is now to overcome its limited surface colonization in the absence of Desulfuromonas spp.

Shelley D Minteer - One of the best experts on this subject based on the ideXlab platform.

  • developing ethanol Bioanodes using a hydrophobically modified linear polyethylenimine hydrogel for immobilizing an enzyme cascade
    Journal of Electroanalytical Chemistry, 2018
    Co-Authors: Sidney Aquino Neto, Shelley D Minteer, Adalgisa Rodrigues De Andrade
    Abstract:

    Abstract Considering the importance of immobilizing dehydrogenase enzymes for bioelectronics, the focus of this paper is to obtain an enzymes cascade for ethanol bioelectrooxidation onto a carbon paper support using a hydrophobically-modified linear polymer of ethyleneimine (LPEI) as the immobilization matrix along with ethylene glycol diglycidyl ether (EGDGE) as the cross-linking agent. In the first stage of ethanol bioelectrooxidation, the biofuel cell tests show that the amount of the cross-linking agent employed in bioanode preparation directly affects their overall bioelectrocatalytic performance. In fact, much higher performance is observed for the bioanode prepared with an intermediate amount of cross-linking agent, reaching maximum current and power densities of respectively 12.5 ± 1.4 μW cm− 2 and 150 ± 17 μA cm− 2. In both the second and third steps of the ethanol oxidation cascade, several optimizations had to be performed in order to obtain a stable and functioning bioanode, but this work shows that the optimal crosslinking conditions are enzyme dependent. Although immobilization methodologies such as hydrogels have a wide application range in bioelectronics, the proportion between LPEI, EGDGE, and the enzymes being immobilized is quite important to obtain a functioning bioanode, particularly when a cascade of enzymes needs to be immobilized into this matrix.

  • hybrid glucose o2 biobattery and supercapacitor utilizing a pseudocapacitive dimethylferrocene redox polymer at the bioanode
    ACS energy letters, 2016
    Co-Authors: Krysti L Knoche, David P. Hickey, Ross D Milton, Carol Lynn Curchoe, Shelley D Minteer
    Abstract:

    Small implantable electronic devices require biologically compatible energy sources that are capable of delivering quick high-energy pulses. Combining batteries and supercapacitors allows for high power and energy density while providing both small size and biocompatibility. Here, we report a hybrid supercapacitor/biobattery whereby an oxygen-reducing cathode of bilirubin oxidase immobilized with anthracene-modified carbon nanotubes and tetrabutylammonium bromide-modified Nafion is coupled with a glucose bioanode of flavin adenine dinucleotide-dependent glucose dehydrogenase. The redox polymer, dimethylferrocene-modified linear poly(ethylenimine), used at the bioanode simultaneously immobilizes enzyme, mediates electron transfer, and acts as a pseudocapacitor where capacitance of the anode scales with increased polymer loading. Both multiwalled carbon nanotubes and carbon felt incorporated into the anode construction improve polymer conductivity, subsequently resulting in further improved anodic capacitan...

  • High current density PQQ-dependent alcohol and aldehyde dehydrogenase Bioanodes
    Biosensors and Bioelectronics, 2015
    Co-Authors: Sidney Aquino Neto, Adalgisa R. De Andrade, David P. Hickey, Ross D Milton, Shelley D Minteer
    Abstract:

    In this paper, we explore the bioelectrooxidation of ethanol using pyrroloquinoline quinone (PQQ)-dependent alcohol and aldehyde dehydrogenase (ADH and AldDH) enzymes for biofuel cell applications. The bioanode architectures were designed with both direct electron transfer (DET) and mediated electron transfer (MET) mechanisms employing high surface area materials such as multi-walled carbon nanotubes (MWCNTs) and MWCNT-decorated gold nanoparticles, along with different immobilization techniques. Three different polymeric matrices were tested (tetrabutyl ammonium bromide (TBAB)-modified Nafion; octyl-modified linear polyethyleneimine (C8-LPEI); and cellulose) in the DET studies. The modified Nafion membrane provided the best electrical communication between enzymes and the electrode surface, with catalytic currents as high as 16.8±2.1μAcm-2. Then, a series of ferrocene redox polymers were evaluated for MET. The redox polymer 1,1'-dimethylferrocene-modified linear polyethyleneimine (FcMe2-C3-LPEI) provided the best electrochemical response. Using this polymer, the electrochemical assays conducted in the presence of MWCNTs and MWCNTs-Au indicated a Jmax of 781±59μAcm-2 and 925±68μAcm-2, respectively. Overall, from the results obtained here, DET using the PQQ-dependent ADH and AldDH still lacks high current density, while the Bioanodes that operate via MET employing ferrocene-modified LPEI redox polymers show efficient energy conversion capability in ethanol/air biofuel cells.

  • direct electron transfer based Bioanodes for ethanol biofuel cells using pqq dependent alcohol and aldehyde dehydrogenases
    Electrochimica Acta, 2013
    Co-Authors: Sidney Aquino Neto, Emily L Suda, Matthew T Meredith, Adalgisa Rodrigues De Andrade, Shelley D Minteer
    Abstract:

    Abstract This paper compares the performance of a DET (direct electron transfer) bioanode containing both PQQ-ADH (pyrroloquinoline quinone-dependent alcohol dehydrogenase) and PQQ-AldDH (PQQ-dependent aldehyde dehydrogenase) immobilized onto different modified electrode surfaces employing either a tetrabutylammonium (TBAB)-modified Nafion ® membrane polymer or polyamidoamine (PAMAM) dendrimers for the enzyme immobilization. The electrochemical characterization showed that the prepared bioelectrodes were able to undergo DET onto glassy carbon surface in the presence as well as the absence of multi-walled carbon nanotubes (MWCNTs); also, in the latter case a relevant shift in the oxidation peak of about 180 mV vs. saturated calomel electrode (SCE) was observed. A very similar redox potential was achieved with the self-assembled bioelectrode prepared onto modified-gold surfaces with dendrimers, indicating that both methodologies provide an environment that enables the PQQ-enzymes to undergo DET. The biofuel cell tests confirmed the ease of the DET process and the enhanced performance in the presence of the carbon nanotubes. Considering the Bioanodes prepared with PAMAM dendrimers, the power density values vary from 19.4 μW cm −2 without MWCNTs to 25.7 μW cm −2 in the presence of MWCNTs. Similarly, with the Bioanodes prepared with the TBAB-modified-Nafion ® polymer, the results indicate power densities of 27.9 and 38.4 μW cm −2 respectively. These electrode modifications represent effective methods for immobilization and direct electrical connection of quinohemoproteins to electrode surfaces.

  • characterization and stability study of immobilized pqq dependent aldose dehydrogenase Bioanodes
    Electroanalysis, 2012
    Co-Authors: Shelley D Minteer, Saara Tuurala, Carolin Lau, Plamen Atanassov, Maria Smolander
    Abstract:

    In this paper, glucose oxidizing Bioanodes employing immobilized PQQ-dependent aldose dehydrogenase were prepared and characterized. The enzyme was immobilized on carbon paper in two different polymeric systems: tetrabutylammonium bromide (TBAB) modified Nafion and butanal modified chitosan. Characterization of the Bioanodes included electron microscopy, electrochemical evaluation, as well as stability and leaching studies. Results indicate that the operational degradation was the same but the long term storage stability is better in the case of modified Nafion. The performance of the modified Nafion immobilized Bioanodes stayed at 70 % of the initial value after 60 days of storing at 4 °C and 25 °C. Compared to TBAB modified Nafion immobilized Bioanodes, butanal modified chitosan immobilized Bioanodes showed 50 % activity after eight weeks storage at 4°C and one week storage at 25 °C. However, the electrochemical properties of modified chitosan were better.

Benjamin Erable - One of the best experts on this subject based on the ideXlab platform.

  • industrially scalable surface treatments to enhance the current density output from graphite Bioanodes fueled by real domestic wastewater
    iScience, 2021
    Co-Authors: Emma Roubaud, Alain Bergel, Luc Etcheverry, Rémy Lacroix, Serge Da Silva, Régine Basséguy, Jerome Esvan, Benjamin Erable
    Abstract:

    Acid and electrochemical surface treatments of graphite electrode, used individually or in combination, significantly improved the microbial anode current production, by +17% to +56%, in well-regulated and duplicated electroanalytical experimental systems. Of all the consequences induced by surface treatments, the modifications of the surface nano-topography preferentially justify an improvement in the fixation of bacteria, and an increase of the specific surface area and the electrochemically accessible surface of graphite electrodes, which are at the origin of the higher performances of the Bioanodes supplied with domestic wastewater. The evolution of the chemical composition and the appearance of C-O, C=O, and O=C-O groups on the graphite surface created by combining acid and electrochemical treatments was prejudicial to the formation of efficient domestic-wastewater-oxidizing Bioanodes. The comparative discussion, focused on the positioning of the performances, shows the industrial interest of applying the surface treatment method to the world of bioelectrochemical systems.

  • allochthonous and autochthonous halothermotolerant Bioanodes from hypersaline sediment and textile wastewater a promising microbial electrochemical process for energy recovery coupled with real textile wastewater treatment
    Frontiers in Bioengineering and Biotechnology, 2020
    Co-Authors: Benjamin Erable, Refka Askri, Mohamed Neifar, Luc Etcheverry, Ameur Cherif, Sirine Saadaoui, Habib Chouchane
    Abstract:

    The textile and clothing industry is the first manufacture sector in Tunisia in terms of employment and number of enterprises. It generates large volumes of textile dyeing wastewater (TDWW) containing high concentrations of saline, alkaline, and recalcitrant pollutants that could fuel tenacious and resilient electrochemically active microorganisms in Bioanodes of bioelectrochemical systems. In this study, a designed hybrid bacterial halothermotolerant bioanode incorporating indigenous and exogenous bacteria from both hypersaline sediment of Chott El Djerid (HSCE) and TDWW is proposed for simultaneous treatment of real TDWW and anodic current generation under high salinity. For the proposed halothermotolerant Bioanodes, electrical current production, chemical oxygen demand (COD) removal efficiency, and bacterial community dynamics were monitored. All the experiments of halothermotolerant bioanode formation have been conducted on 6 cm2 carbon felt electrodes polarized at −0.1 V/SCE and inoculated with 80% of TDWW and 20% of HSCE for 17 days at 45°C. A reproducible current production of about 12.5 ± 0.2 A/m2 and a total of 91 ± 3% of COD removal efficiency were experimentally validated. Metagenomic analysis demonstrated significant differences in bacterial diversity mainly at species level between anodic biofilms incorporating allochthonous and autochthonous bacteria and anodic biofilm containing only autochthonous bacteria as a control. Therefore, we concluded that these results provide for the first time a new noteworthy alternative for achieving treatment and recover energy, in the form of a high electric current, from real saline TDWW.

  • Benchmarking of Industrial Synthetic Graphite Grades, Carbon Felt, and Carbon Cloth as Cost-Efficient Bioanode Materials for Domestic Wastewater Fed Microbial Electrolysis Cells
    Frontiers in Energy Research, 2019
    Co-Authors: Emma Roubaud, Alain Bergel, Luc Etcheverry, Rémy Lacroix, Serge Da Silva, Régine Basséguy, Benjamin Erable
    Abstract:

    Anode material selection is crucial when it comes to building up-scaled microbialelectrolysis cells (MEC), as it has a huge influence on the achievable current density and account for a large part of the MEC total investment cost. Graphite is a material that isperfectly suited to the creation of up-scaled Bioanodes as it is conductive, chemically stable, biocompatible, and relatively cheap but there are a very large number of commercially available grades of industrial graphite. In this study, five grades of industrial synthetic graphite (named G1–G5) were bench tested to select the most suitable gradefor future development of 3D bioanode for domestic wastewater (dWW) fed MEC application. The five grades of graphite have been selected with similar physico-chemicaland surface properties (electrical resistivity, surface roughness, and hydrophobicity) theoretically appropriate for EA biofilm development. Nevertheless, significant current density disparities where observed with the five graphite grades, which can certainlybe explained by the fabrication procedures of the respective material grades. With thegraphite grade giving the most efficient anodes (G3), an average steady state currentdensity of 2.3 A/m²was produced, outperforming the other grades by at least 15%.Even though all graphites had very close physico-chemical characteristics, the gradehad a clear significant influence on the current densities produced. G3 graphite was finally compared to carbon felt (CF) and carbon cloth (CC) both in terms of bio-electrochemicalcurrent production and bacterial communities colonizing electrodes. G3 Bioanodes outperformed CF and CC Bioanodes by 50% in term of steady state current density.Biofilms microbial population analysis showed that theGeobacterspecies was presentat 82% on G3 Bioanodes, 39% on CF Bioanodes, and 61% on CC Bioanodes when it was only present at 0.06% in the activated sludge used as inoculum. This significant difference in bacterial enrichment could come from the huge gap between materials resistivity, as graphite resistivity is 200-fold lower than CF and CC resistivities. The strongly hydrophilic surface of G3 graphite was also certainly beneficial for biofilm development compared tothe hydrophobic surfaces of CF and CC.

  • Understanding the cumulative effects of salinity, temperature and inoculation size for the design of optimal halothermotolerant Bioanodes from hypersaline sediments
    Bioelectrochemistry, 2019
    Co-Authors: Refka Askri, Benjamin Erable, Mohamed Neifar, Luc Etcheverry, Ahmed Slaheddine Masmoudi, Ameur Cherif, Habib Chouchane
    Abstract:

    The main objective of this study was to understand the interaction between salinity, temperature and inoculum size and how it could lead to the formation of efficient halothermotolerant Bioanodes from the Hypersaline Sediment of Chott El Djerid (HSCE). Sixteen experiments on bioanode formation were designed using a Box-Behnken matrix and response surface methodology to understand synchronous interactions. All bioanode formations were conducted on 6 cm2 carbon felt electrodes polarized at −0.1 V/SCE and fed with lactate (5 g/L) at pH 7.0. Optimum levels for salinity, temperature and inoculum size were predicted by NemrodW software as 165 g/L, 45 °C and 20%, respectively, under which conditions maximum current production of 6.98 ± 0.06 A/m2 was experimentally validated. Metagenomic analysis of selected biofilms indicated a relative abundance of the two phyla Proteobacteria (from 85.96 to 89.47%) and Firmicutes (from 61.90 to 68.27%). At species level, enrichment of Psychrobacter aquaticus, Halanaerobium praevalens, Psychrobacter alimentaris, and Marinobacter hydrocarbonoclasticus on carbon-based electrodes was correlated with high current production, high salinity and high temperature. Members of the halothermophilic bacteria pool from HSCE, individually or in consortia, are candidates for designing halothermotolerant Bioanodes applicable in the bioelectrochemical treatment of industrial wastewater at high salinity and temperature.

  • Increasing the temperature is a relevant strategy to form microbial anodes intended to work at room temperature
    Electrochimica Acta, 2017
    Co-Authors: Manon Oliot, Benjamin Erable, Marie-line De Solan, Alain Bergel
    Abstract:

    Reducing the time required for the formation of microbial anodes from environmental inocula is a great challenge. The possibility of reaching this objective by increasing the temperature during the bioanode preparation was investigated here. Microbial anodes were formed at 25 °C and 40 °C under controlled potential with successive acetate additions. At 25 °C, around 40 days were required to perform three acetate batches, which led to current density of 9.4 ± 2 A.m−2, while at 40 °C, 20 days were sufficient to complete three similar batches, leading to 22.9 ± 4.2 A.m−2. The Bioanodes formed at 40 °C revealed three redox systems and those formed at 25 °C only one. The temperature also impacted the biofilm structure, which was less compact at 40 °C. When the Bioanodes formed at 40 °C were switched to 25 °C, they produced current densities similar to those of Bioanodes formed at 25 °C; they recovered the single redox system that was developed by the Bioanodes formed at 25 °C and the difference in biofilm structures was mitigated. It is consequently fully appropriate to accelerate the formation of microbial anodes by increasing the temperatures to 40 °C even if they are finally intended to operate at room temperature.

Adrian Ruff - One of the best experts on this subject based on the ideXlab platform.

  • redox polymer wired nifese hydrogenase variants with enhanced o2 stability for triple protected high current density h2 oxidation Bioanodes
    Chemsuschem, 2020
    Co-Authors: Adrian Ruff, Julian Szczesny, Sonia Zacarias, Inês A. C. Pereira, Maria Jose Vega, Pedro M Matias, Sebastien Gounel, Nicolas Mano, Wolfgang Schuhmann
    Abstract:

    Variants of the highly active [NiFeSe] hydrogenase from D. vulgaris Hildenborough that exhibit enhanced O2 tolerance were used as H2 -oxidation catalysts in H2 /O2 biofuel cells. Two [NiFeSe] variants were electrically wired by means of low-potential viologen-modified redox polymers and evaluated with respect to H2 -oxidation and stability against O2 in the immobilized state. The two variants showed maximum current densities of (450±84) μA cm-2 for G491A and (476±172) μA cm-2 for variant G941S on glassy carbon electrodes and a higher O2 tolerance than the wild type. In addition, the polymer protected the enzyme from O2 damage and high-potential inactivation, establishing a triple protection for the bioanode. The use of gas-diffusion Bioanodes provided current densities for H2 -oxidation of up to 6.3 mA cm-2 . Combination of the gas-diffusion bioanode with a bilirubin oxidase-based gas-diffusion O2 -reducing biocathode in a membrane-free biofuel cell under anode-limiting conditions showed unprecedented benchmark power densities of 4.4 mW cm-2 at 0.7 V and an open-circuit voltage of 1.14 V even at moderate catalyst loadings, outperforming the previously reported system obtained with the [NiFeSe] wild type and the [NiFe] hydrogenase from D. vulgaris Miyazaki F.

  • redox polymer based high current density gas diffusion h2 oxidation bioanode using fefe hydrogenase from desulfovibrio desulfuricans in a membrane free biofuel cell
    Angewandte Chemie, 2020
    Co-Authors: Julian Szczesny, Felipe Conzuelo, Wolfgang Lubitz, Adrian Ruff, James A Birrell, Wolfgang Schuhmann
    Abstract:

    The incorporation of highly active but also highly sensitive catalysts (e.g. the [FeFe] hydrogenase from Desulfovibrio desulfuricans) in biofuel cells is still one of the major challenges in sustainable energy conversion. We report the fabrication of a dual-gas diffusion electrode H2 /O2 biofuel cell equipped with a [FeFe] hydrogenase/redox polymer-based high-current-density H2 -oxidation bioanode. The Bioanodes show benchmark current densities of around 14 mA cm-2 and the corresponding fuel cell tests exhibit a benchmark for a hydrogenase/redox polymer-based biofuel cell with outstanding power densities of 5.4 mW cm-2 at 0.7 V cell voltage. Furthermore, the highly sensitive [FeFe] hydrogenase is protected against oxygen damage by the redox polymer and can function under 5 % O2 .

  • a gas breathing hydrogen air biofuel cell comprising a redox polymer hydrogenase based bioanode
    Nature Communications, 2018
    Co-Authors: Julian Szczesny, Wolfgang Schuhmann, Felipe Conzuelo, Sonia Zacarias, Inês A. C. Pereira, Wolfgang Lubitz, Nicolas Plumeré, Nikola Markovic, Adrian Ruff
    Abstract:

    Hydrogen is one of the most promising alternatives for fossil fuels. However, the power output of hydrogen/oxygen fuel cells is often restricted by mass transport limitations of the substrate. Here, we present a dual-gas breathing H2/air biofuel cell that overcomes these limitations. The cell is equipped with a hydrogen-oxidizing redox polymer/hydrogenase gas-breathing bioanode and an oxygen-reducing bilirubin oxidase gas-breathing biocathode (operated in a direct electron transfer regime). The bioanode consists of a two layer system with a redox polymer-based adhesion layer and an active, redox polymer/hydrogenase top layer. The redox polymers protect the biocatalyst from high potentials and oxygen damage. The Bioanodes show remarkable current densities of up to 8 mA cm-2. A maximum power density of 3.6 mW cm-2 at 0.7 V and an open circuit voltage of up to 1.13 V were achieved in biofuel cell tests, representing outstanding values for a device that is based on a redox polymer-based hydrogenase bioanode.

  • a fully protected hydrogenase polymer based bioanode for high performance hydrogen glucose biofuel cells
    Nature Communications, 2018
    Co-Authors: Adrian Ruff, Julian Szczesny, Felipe Conzuelo, Sonia Zacarias, Inês A. C. Pereira, Wolfgang Lubitz, Nikola Markovic, Wolfgang Schuhmann
    Abstract:

    Hydrogenases with Ni- and/or Fe-based active sites are highly active hydrogen oxidation catalysts with activities similar to those of noble metal catalysts. However, the activity is connected to a sensitivity towards high-potential deactivation and oxygen damage. Here we report a fully protected polymer multilayer/hydrogenase-based bioanode in which the sensitive hydrogen oxidation catalyst is protected from high-potential deactivation and from oxygen damage by using a polymer multilayer architecture. The active catalyst is embedded in a low-potential polymer (protection from high-potential deactivation) and covered with a polymer-supported bienzymatic oxygen removal system. In contrast to previously reported polymer-based protection systems, the proposed strategy fully decouples the hydrogenase reaction form the protection process. Incorporation of the bioanode into a hydrogen/glucose biofuel cell provides a benchmark open circuit voltage of 1.15 V and power densities of up to 530 µW cm−2 at 0.85 V. Hydrogenases are promising alternatives to noble metal-based catalysts for hydrogen oxidation. Here the authors fully protect air-sensitive hydrogenases from high potential and oxygen damage using a polymer multilayer bioanode in a biofuel cell that delivers a benchmark open circuit voltage.

Lo Gorton - One of the best experts on this subject based on the ideXlab platform.

  • A Glucose/Oxygen Enzymatic Fuel Cell based on Gold Nanoparticles modified Graphene Screen-Printed Electrode. Proof-of-Concept in Human Saliva
    'Elsevier BV', 2018
    Co-Authors: Bollella Paolo, Lo Gorton, Fusco Giovanni, Stevar Daniela, Ludwig Roland, Boer Harry, Koivula Anu, Tortolini Cristina, Favero Gabriele
    Abstract:

    This paper presents a new direct electron transfer based-miniaturized glucose/oxygen enzymatic fuel cell (EFC) whose operating ability has been tested in real saliva samples. The bioanode and biocathode are a graphene working electrode and a graphite counter electrode localized on the same screen printed electrode (SPE) modified with poly(vinyl alcohol) N-methyl-4(4'-formylstyryl)pyridinium methosulfate acetal (PVA-SbQ)/cellobiose dehydrogenase from Corynascus Thermophilus (CtCDH) C291Y/AuNPs and with Trametes Hirsuta laccase (ThLac)/AuNPs, respectively.In order to optimize the bioanode, several CDH immobilization procedures were adopted, such as drop-casting, use of Nafion membrane or PVA-SbQ photopolymer. The photopolymer showed the best performance in terms of stability and reliability. As biocathode a partially optimized laccase electrode was employed with the variant that the used nanomaterials allowed to reduce the overpotential of O2/H2O redox reaction catalyzed by Trametes Hirsuta Laccase (ThLac), drop-casted onto the gold nanoparticles (AuNPs) modified SPE.The performances of bioanode and biocathode were tested separately, initially immobilizing the two enzymes onto separated graphene SPEs. An efficient direct electron transfer was achieved for both elements, obtaining an apparent heterogeneous electron transfer rate constant (ks ) of 0.99±0.05s-1 for CtCDH C291Y and 5.60±0.05s-1 for ThLac. Both electrodes were then assembled in a two compartment EFC obtaining a maximal power output of 5.16±0.15μWcm-2 at a cell voltage of 0.58V and an open circuit voltage (OCV) of 0.74V. Successively, the bioanode and biocathode were assembled in a non-compartmentalized EFC and a remarkable 50% decrease of the maximum power output at the value of 2.15±0.12μWcm-2 at cell voltage of 0.48V and an OCV of 0.62V at pH 6.5 was registered. In order to reduce the cell dimensions in view of its possible integration in biomedical devices, the bioanode and biocaythode were realized by immobilization of both enzymes onto the same SPE. The so miniaturized EFC delivered a maximal power output of 1.57±0.07μWcm2 and 1.10±0.12μWcm-2 with an OCV of 0.58V and 0.41V in a 100μM glucose solution and in human saliva, respectively

  • development of a bioanode for microbial fuel cells based on the combination of a mwcnt au pt hybrid nanomaterial an osmium redox polymer and gluconobacter oxydans dsm 2343 cells
    ChemistrySelect; 2(36) pp 12034-12040 (2017), 2017
    Co-Authors: Sema Aslan, Peter O Conghaile, Donal Leech, Lo Gorton, Suna Timur, Ulku Anik
    Abstract:

    In this work, a carbon felt electrode (CFE) was modified with a multiwalled carbon nanotube-gold-platinum (MWCNT-Au-Pt) hybrid nanomaterial and integrated with an osmium redox polymer (OsRP, [Os(2, 2’-bipyridine)2(poly-vinylimidazole)10Cl]Cl) and Gluconobacter oxydans DSM 2343 (G. oxydans) cells. The developed electrode was used as the bioanode in a 5.0 mM K3Fe(CN)6mediator containing phosphate buffer (pH 6.5) anolyte and combined with a Pt wire cathode in phosphoric acid medium (pH 3.5). As a result, a two chamber microbial fuel cell (MFC) was formed, in which an activated Nafion membrane was used as a proton exchange membrane. The OsRP/G.oxydans/MWCNT-Au-Pt/CFE based bioanode was electrochemically examined in differently deoxygenated bioanode chambers and additionally the amounts of hybrid nanomaterial and OsRP were optimized. In terms of MFC characteristics, it was found that an anaerobic OsRP/G.oxydans/MWCNT-Au-Pt/CFE bioanode based MFC had a maximum power density of 32.1 mW m-2(at 90 mV), a maximum current density of 1032 mA m-2and a charge transfer efficiency (E%) value of 22.30 (open circuit potential 180 mV).

  • development of an osmium redox polymer mediated bioanode and examination of its performance in gluconobacter oxydans based microbial fuel cell
    Electroanalysis, 2017
    Co-Authors: Sema Aslan, Peter O Conghaile, Donal Leech, Lo Gorton, Suna Timur, Ulku Anik
    Abstract:

    Gluconobacter oxydans (G. oxydans) cells together with an osmium redox polymer (ORP) [Osmium (2,2'-bipyridine)2(poly-vinylimidazole)10Cl]Cl were combined with a glassy carbon paste electrode (GCPE) to form a bioanode for a microbial fuel cell (MFC) based on G. oxydans. Although there are G.oxydans/ ORP combined bioanode in the literature, as far as it is known, this system is the first one where G.oxydans/ORP bioanode is combined with a cathode and a MFC is formed. After the optimization of experimental parameters, analytical characteristics of ORP/G. oxydans/GCPE bioanode were investigated. ORP/G. oxydans/GCPE showed two linear ranges for ethanol substrate as 1.0-30mM (R2=0.902) and 30-500mM (R2=0.997) and analytical range as 1.0-1000mM. Limit of detection (3.0s/m) and limit of quantification (10s/m) values were calculated as 1.29mM and 4.30mM respectively where the RSD value was 1.16% for n=5. Combining the developed bioanode in the presence of 5.0mM K3Fe(CN)6 mediator with a Pt wire cathode a double compartment MFC was obtained via a salt bridge. G. oxydans/GCPE bioanode based MFC had maximum power density of 0.133 μW cm-2 (at 33.5 mV), maximum current density as 8.73 μA cm-2 and OCP value of 156 mV. On the other hand, ORP/G. oxydans/GCPE based MFC showed maximum power density as 0.26 μW cm-2 (at 46.8 mV), maximum current density as 15.079 μA cm-2 and OCP value of 176 mV. (Less)

  • supercapacitive photo Bioanodes and biosolar cells a novel approach for solar energy harnessing
    Advanced Energy Materials, 2017
    Co-Authors: Galina Pankratova, Sergey Shleev, Donal Leech, Dmitry Pankratov, Kamrul Hasan, Hanserik Akerlund, Perake Albertsson, Lo Gorton
    Abstract:

    The concept of supercapacitive photo-bioanode and biosolar cell (photo-biosupercapacitor) for simultaneous solar energy conversion and storage is demonstrated for the first time. Exploiting the cap ...

  • Development of an Osmium Redox Polymer Mediated Bioanode and Examination of its Performance in Gluconobacter oxydans Based Microbial Fuel Cell
    'Wiley', 2017
    Co-Authors: Aslan Sema, Lo Gorton, Conghaile, Peter O., Leech Donal, Timur Suna, Anik Ulku
    Abstract:

    WOS: 000405309000020Gluconobacter oxydans (G. oxydans) cells together with an osmium redox polymer (ORP) [Osmium (2,2'-bipyridine) 2(poly-vinylimidazole) 10Cl] Cl were combined with a glassy carbon paste electrode (GCPE) to form a bioanode for a microbial fuel cell (MFC) based on G. oxydans. Although there are G. oxydans/ORP combined bioanode in the literature, as far as it is known, this system is the first one where G. oxydans/ORP bioanode is combined with a cathode and a MFC is formed. After the optimization of experimental parameters, analytical characteristics of ORP/G. oxydans/GCPE bioanode were investigated. ORP/G. oxydans/GCPE showed two linear ranges for ethanol substrate as 1.0-30 mM (R-2 = 0.902) and 30-500 mM (R-2 = 0.997) and analytical range as 1.0-1000 mM. Limit of detection (3.0 s/m) and limit of quantification (10 s/m) values were calculated as 1.29 mM and 4.30 mM respectively where the RSD value was 1.16% for n=5. Combining the developed bioanode in the presence of 5.0 mM K3Fe(CN) 6 mediator with a Pt wire cathode a double compartment MFC was obtained via a salt bridge. G. oxydans/GCPE bioanode based MFC had maximum power density of 0.133 mW cm(-2) (at 33.5 mV), maximum current density as 8.73 mA cm(-2) and OCP value of 156 mV. On the other hand, ORP/G. oxydans/GCPE based MFC showed maximum power density as 0.26 mW cm(-2) (at 46.8 mV), maximum current density as 15.079 mA cm(-2) and OCP value of 176 mV