The Experts below are selected from a list of 1035 Experts worldwide ranked by ideXlab platform
Vitaly J. Vodyanoy - One of the best experts on this subject based on the ideXlab platform.
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The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor
Sensing for Agriculture and Food Quality and Safety III, 2011Co-Authors: Yating Chai, Vitaly J. Vodyanoy, Shin Horikawa, Wen Shen, Mi-kyung Park, Bryan A. ChinAbstract:This paper presents the direct detection of Salmonella typhimurium on shell eggs using a phage-based magnetoelastic (ME) biosensor. The ME biosensor consists of a ME resonator as the sensor platform and E2 phage as the Biorecognition Element that is genetically engineered to specifically bind with Salmonella typhimurium . The ME biosensor, which is a wireless sensor, vibrates with a characteristic resonant frequency under an externally applied magnetic field. Multiple sensors can easily be remotely monitored. Multiple measurement and control sensors were placed on the shell eggs contaminated by Salmonella typhimurium solutions with different known concentrations. The resonant frequency of sensors before and after the exposure to the spiked shell eggs was measured. The frequency shift of the measurement sensors was significantly different than the control sensors indicating Salmonella contamination. Scanning electron microscopy was used to confirm binding of Salmonella to the sensor surface and the resulting frequency shift results.
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lytic phage as a specific and selective probe for detection of staphylococcus aureus a surface plasmon resonance spectroscopic study
Biosensors and Bioelectronics, 2007Co-Authors: Shankar Balasubramanian, Vitaly J. Vodyanoy, Iryna Sorokulova, Aleksandr L SimonianAbstract:Abstract Rapid and reliable detection of harmful pathogens at low levels are vital due to the related environmental and economical impact. While antibodies (monoclonal or polyclonal) are successfully employed in many immunoanalysis procedures as a Biorecognition Element, many of them remain costly with a comparatively short shelf life and uncertain manufacturability. Additionally, they suffer from several limitations, such as susceptibility to hostile environmental stresses such as temperature, pH, ionic strength, and cross-reactivity. The development of easy available, sensitive, and robust alternative molecular recognition Elements, capable of providing a very high level of selectivity are very attractive to industry and may benefit in multiple areas. Several attempts have been made to utilize fluorescent-tagged bacteriophages and phage-displayed peptides for bacterial detection. However, involvement of complex labeling and detecting procedures make these approaches time-consuming and complicated. Here, we are reporting for the first time, the label-free detection of Staphylococcus aureus using lytic phage as highly specific and selective Biorecognition Element and surface plasmon resonance-based SPREETA™ sensor as a detection platform. Lytic phage was immobilized on the gold surface of SPREETA sensor via trouble-free direct physical adsorption. The detection limit was found to be 10 4 cfu/ml. Detection specificity was investigated by an inhibition assay while selectivity was examined with Salmonella typhimurium . The preliminary results using lytic phage as a probe for bacterial detection, in combination with SPR platform are promising and hence can be employed for rapid and label-free detection of different bacterial pathogens.
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Lytic phage as a specific and selective probe for detection of Staphylococcus aureus—A surface plasmon resonance spectroscopic study
Biosensors and Bioelectronics, 2006Co-Authors: Shankar Balasubramanian, Iryna Sorokulova, Vitaly J. VodyanoyAbstract:Abstract Rapid and reliable detection of harmful pathogens at low levels are vital due to the related environmental and economical impact. While antibodies (monoclonal or polyclonal) are successfully employed in many immunoanalysis procedures as a Biorecognition Element, many of them remain costly with a comparatively short shelf life and uncertain manufacturability. Additionally, they suffer from several limitations, such as susceptibility to hostile environmental stresses such as temperature, pH, ionic strength, and cross-reactivity. The development of easy available, sensitive, and robust alternative molecular recognition Elements, capable of providing a very high level of selectivity are very attractive to industry and may benefit in multiple areas. Several attempts have been made to utilize fluorescent-tagged bacteriophages and phage-displayed peptides for bacterial detection. However, involvement of complex labeling and detecting procedures make these approaches time-consuming and complicated. Here, we are reporting for the first time, the label-free detection of Staphylococcus aureus using lytic phage as highly specific and selective Biorecognition Element and surface plasmon resonance-based SPREETA™ sensor as a detection platform. Lytic phage was immobilized on the gold surface of SPREETA sensor via trouble-free direct physical adsorption. The detection limit was found to be 10 4 cfu/ml. Detection specificity was investigated by an inhibition assay while selectivity was examined with Salmonella typhimurium . The preliminary results using lytic phage as a probe for bacterial detection, in combination with SPR platform are promising and hence can be employed for rapid and label-free detection of different bacterial pathogens.
Shankar Balasubramanian - One of the best experts on this subject based on the ideXlab platform.
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lytic phage as a specific and selective probe for detection of staphylococcus aureus a surface plasmon resonance spectroscopic study
Biosensors and Bioelectronics, 2007Co-Authors: Shankar Balasubramanian, Vitaly J. Vodyanoy, Iryna Sorokulova, Aleksandr L SimonianAbstract:Abstract Rapid and reliable detection of harmful pathogens at low levels are vital due to the related environmental and economical impact. While antibodies (monoclonal or polyclonal) are successfully employed in many immunoanalysis procedures as a Biorecognition Element, many of them remain costly with a comparatively short shelf life and uncertain manufacturability. Additionally, they suffer from several limitations, such as susceptibility to hostile environmental stresses such as temperature, pH, ionic strength, and cross-reactivity. The development of easy available, sensitive, and robust alternative molecular recognition Elements, capable of providing a very high level of selectivity are very attractive to industry and may benefit in multiple areas. Several attempts have been made to utilize fluorescent-tagged bacteriophages and phage-displayed peptides for bacterial detection. However, involvement of complex labeling and detecting procedures make these approaches time-consuming and complicated. Here, we are reporting for the first time, the label-free detection of Staphylococcus aureus using lytic phage as highly specific and selective Biorecognition Element and surface plasmon resonance-based SPREETA™ sensor as a detection platform. Lytic phage was immobilized on the gold surface of SPREETA sensor via trouble-free direct physical adsorption. The detection limit was found to be 10 4 cfu/ml. Detection specificity was investigated by an inhibition assay while selectivity was examined with Salmonella typhimurium . The preliminary results using lytic phage as a probe for bacterial detection, in combination with SPR platform are promising and hence can be employed for rapid and label-free detection of different bacterial pathogens.
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Lytic phage as a specific and selective probe for detection of Staphylococcus aureus—A surface plasmon resonance spectroscopic study
Biosensors and Bioelectronics, 2006Co-Authors: Shankar Balasubramanian, Iryna Sorokulova, Vitaly J. VodyanoyAbstract:Abstract Rapid and reliable detection of harmful pathogens at low levels are vital due to the related environmental and economical impact. While antibodies (monoclonal or polyclonal) are successfully employed in many immunoanalysis procedures as a Biorecognition Element, many of them remain costly with a comparatively short shelf life and uncertain manufacturability. Additionally, they suffer from several limitations, such as susceptibility to hostile environmental stresses such as temperature, pH, ionic strength, and cross-reactivity. The development of easy available, sensitive, and robust alternative molecular recognition Elements, capable of providing a very high level of selectivity are very attractive to industry and may benefit in multiple areas. Several attempts have been made to utilize fluorescent-tagged bacteriophages and phage-displayed peptides for bacterial detection. However, involvement of complex labeling and detecting procedures make these approaches time-consuming and complicated. Here, we are reporting for the first time, the label-free detection of Staphylococcus aureus using lytic phage as highly specific and selective Biorecognition Element and surface plasmon resonance-based SPREETA™ sensor as a detection platform. Lytic phage was immobilized on the gold surface of SPREETA sensor via trouble-free direct physical adsorption. The detection limit was found to be 10 4 cfu/ml. Detection specificity was investigated by an inhibition assay while selectivity was examined with Salmonella typhimurium . The preliminary results using lytic phage as a probe for bacterial detection, in combination with SPR platform are promising and hence can be employed for rapid and label-free detection of different bacterial pathogens.
Adelio Rigo - One of the best experts on this subject based on the ideXlab platform.
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A high sensitivity amperometric biosensor using laccase as Biorecognition Element
Biosensors & bioelectronics, 2005Co-Authors: Fabio Vianello, Santa Ragusa, Maria Teresa Cambria, Adelio RigoAbstract:An amperometric flow biosensor, using laccase from Rigidoporus lignosus as bioElement was developed. The laccase was kinetically characterized towards various phenolics both in solution and immobilized to a hydrophilic matrix by carbodiimide chemistry. A bioreactor connected to an amperometric flow cell by a FIA system was filled with the immobilized enzyme and the operational conditions of this biosensor were optimized as regards pH. Under the adopted experimental conditions, the immobilized enzyme oxidizes all the substrate molecules avoiding the need of cumbersome calibration procedures. The biosensor sensitivity, which was found to be 100 nA/microM for some of the tested substrates, resulted to be constant for more than 100 working days. This biosensor permits the detection of phenolics in aqueous solutions at concentrations in the nanomolar range and was successfully used to detect phenolics in wastewaters from olive oil mill without sample preparation.
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A high sensitivity amperometric biosensor using a monomolecular layer of laccase as Biorecognition Element
Biosensors & bioelectronics, 2004Co-Authors: Fabio Vianello, Santa Ragusa, Maria Teresa Cambria, Antonio Cambria, Lucio Zennaro, Adelio RigoAbstract:Abstract Laccases from various sources were tested, and laccase from Rigidoporus lignosus was found to be the most active towards syringaldazine and ABTS, which are typical substrates of this class of enzymes, and towards the phenols found in olive oil mill wastewaters. This laccase was covalently immobilised by carbodiimide chemistry, on a self-assembled monolayer of 3-mercaptopropionic acid deposited on a gold surface. A flow biosensor, using the monolayer of laccase as bioElement and a glassy carbon electrode as amperometric transduction system, was developed. Although the amount of the immobilised enzyme (about 140 ng/cm 2 effective surface area) was tiny, the biosensor showed a sensitivity of 3 nA/μM when 1,4-hydroquinone was used as substrate, and a half-life of 35 days. The proposed device permits detection of phenols in aqueous solutions at concentrations in the low micromolar range, i.e. below European Community limits. The biosensor was successfully used to detect phenols in wastewaters from an olive oil mill after minimal sample preparation (incubation of the aqueous sample with sodium borohydride for a few minutes) to suppress the current due to oxidised compounds present in the wastewaters.
Nicole Jaffrezic-renault - One of the best experts on this subject based on the ideXlab platform.
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A High sensitivity impedimetric biosensor using the tannin from quercusmacrolepis as Biorecognition Element for heavy metals detection
2015Co-Authors: Nawal Khedimallah, Ali Zazoua, Amel Sbartai, Nicole Jaffrezic-renaultAbstract:A new poly(vinylchloride) (PVC) membrane electrode based on tannin from the bark of Quercusmacrolepis (acorn) as the ionophore was prepared and modified onto the surface of a gold electrode. The electrochemical impedance spectroscopy (EIS) technique was used to study the sensitivity of the electrode that was modified with a thin layer of polymeric biomembrane, in order to detect heavy metals ions in solution. The device shows a good sensitivity for Zn2+, Ni2+ ions and a little less for Cd2+. The electrode indicates a good linear response for the three metals over a wide concentration range from 1.0 x 10(-19) to 1.0 x 10(-4) M, with a detection limit of 1.0 x 10(-9) M.
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A High Sensitivity Impedimetric Biosensor Using the Tannin From Quercusmacrolepis as Biorecognition Element for Heavy Metals Detection
IEEE transactions on nanobioscience, 2015Co-Authors: Nawal Khedimallah, Ali Zazoua, Amel Sbartai, Nicole Jaffrezic-renaultAbstract:A new poly(vinylchloride) (PVC) membrane electrode based on tannin from the bark of Quercusmacrolepis (acorn) as the ionophore was prepared and modified onto the surface of a gold electrode. The electrochemical impedance spectroscopy (EIS) technique was used to study the sensitivity of the electrode that was modified with a thin layer of polymeric biomembrane, in order to detect heavy metals ions in solution. The device shows a good sensitivity for ${\rm Zn}^{2+}$ , ${\rm Ni}^{2+}$ ions and a little less for ${\rm Cd}^{2+}$ . The electrode indicates a good linear response for the three metals over a wide concentration range from $1.0\times 10^{-9}$ to $1.0\times 10^{-4}\ {\rm M}$ , with a detection limit of $1.0\times 10^{-9}\ {\rm M}$ .
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Enzyme biosensor for tomatine detection in tomatoes
Analytical Letters, 2004Co-Authors: Sergei Dzyadevych, Valentyna Arkhypova, Alexey Soldatkin, Anna El'skaya, Claude Martelet, Nicole Jaffrezic-renaultAbstract:A biosensor for detection of tomatine in tomatoes has been developed using pH-sensitive field effect transistor as transducer and immobilised enzyme butyryl cholinesterase (BuChE) as a Biorecognition Element. The main analytical characteristics of the biosensor developed were studied for different conditions. The possibility to optimise these working parameters was investigated. By using this biosensor and enzyme inhibition effect, the tomatine can be measured in the concentration range of 0.5-50 muM with a detection limit of 0.2 muM. Tomatine concentrations in different tomato juice samples were determined by such a biosensor, and a good correlation with the known real content was revealed. A high reproducibility and operational stability of the biosensor developed were shown.
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A novel enzyme biosensor for steroidal glycoalkaloids detection based on pH-sensitive field effect transistors.
Bioelectrochemistry (Amsterdam Netherlands), 2002Co-Authors: Y I Korpan, Anna El'skaya, Claude Martelet, Nicole Jaffrezic-renault, V V Volotovsky, E A Nazarenko, Alexey SoldatkinAbstract:For the design of a biosensor sensitive to steroidal glycoalkaloids, pH-Sensitive Field Effect Transistors as transducers and immobilised butyrylcholinesterase as a Biorecognition Element have been used. The total potato glycoalcaloids can be measured by this biosensor in the concentration range 0.5-100 microM with detection limits of 0.5 microM for alpha-chaconine and of 2.0 microM for alpha-solanine and solanidine, respectively. The responses of the developed biosensors were reproducible with a relative standard deviation of about 1.5% and 5% for intra- and inter-sensor responses (both cases, n=10, for an alkaloid concentration of 5 microM), respectively. Moreover, due to the reversibility of the enzyme inhibition, the same sensor chip with immobilised butyrylcholinesterase can be used several times (for at least 100 measurements) after a simple washing by a buffer solution and can be stored at 4 degrees C for at least 3 months without any significant loss of the enzymatic activity.
Aleksandr L Simonian - One of the best experts on this subject based on the ideXlab platform.
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lytic phage as a specific and selective probe for detection of staphylococcus aureus a surface plasmon resonance spectroscopic study
Biosensors and Bioelectronics, 2007Co-Authors: Shankar Balasubramanian, Vitaly J. Vodyanoy, Iryna Sorokulova, Aleksandr L SimonianAbstract:Abstract Rapid and reliable detection of harmful pathogens at low levels are vital due to the related environmental and economical impact. While antibodies (monoclonal or polyclonal) are successfully employed in many immunoanalysis procedures as a Biorecognition Element, many of them remain costly with a comparatively short shelf life and uncertain manufacturability. Additionally, they suffer from several limitations, such as susceptibility to hostile environmental stresses such as temperature, pH, ionic strength, and cross-reactivity. The development of easy available, sensitive, and robust alternative molecular recognition Elements, capable of providing a very high level of selectivity are very attractive to industry and may benefit in multiple areas. Several attempts have been made to utilize fluorescent-tagged bacteriophages and phage-displayed peptides for bacterial detection. However, involvement of complex labeling and detecting procedures make these approaches time-consuming and complicated. Here, we are reporting for the first time, the label-free detection of Staphylococcus aureus using lytic phage as highly specific and selective Biorecognition Element and surface plasmon resonance-based SPREETA™ sensor as a detection platform. Lytic phage was immobilized on the gold surface of SPREETA sensor via trouble-free direct physical adsorption. The detection limit was found to be 10 4 cfu/ml. Detection specificity was investigated by an inhibition assay while selectivity was examined with Salmonella typhimurium . The preliminary results using lytic phage as a probe for bacterial detection, in combination with SPR platform are promising and hence can be employed for rapid and label-free detection of different bacterial pathogens.