The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform

Hong-yuan Chen - One of the best experts on this subject based on the ideXlab platform.

  • ECl Color Devices Based on Bipolar Electrode
    2018
    Co-Authors: Hong-yuan Chen
    Abstract:

    ECL is a powerful tool for the study of electron transfer pathways and development of luminescent devices. Ru(II) and Ir(III) luminophores are the most commonly used concomitant electrochemiluminophores, owing to their high ECL efficiencies with TPrA as the co-reactant. Although the selective excitation of electrochemiluminophores was highly expected to open new avenues for multianalyte detection, over the years its application in this area was still limited. The major drawback is the weak solubility of Ir complexes in water, which limits the biological application of multi-color ECL. We adopted closed Bipolar Electrode (BPE), which solved the challenge by separating organic ECL emitters from the analyte. Selective excitation of the electrochemiluminophores (Ru(II) and Ir(III) luminophores) could be achieved by tuning the interfacial potential at the poles of BPE. Along with the increasing potential, the ECL intensities of the two emitters varied, and the emission color changed, which enabled us to detect biomarkers on a BPE array with different strategies. With a single DC power supply, prostate-specific antigen (PSA), circulating microRNA-141 (mRNA-141) and small molecular marker, sarcosine were simultaneously detected by the array.

  • Visual electrochemiluminescence ratiometry on Bipolar Electrode for bioanalysis.
    Biosensors & bioelectronics, 2017
    Co-Authors: Wei Zhao, Hong-yuan Chen
    Abstract:

    Abstract In this work, we developed a visual ECL ratiometry on a closed Bipolar Electrode (BPE) for the detection of prostate specific antigen (PSA), prostate cancer biomarker. High efficient CdTe QDs was synthesized which emitted visualized red light at BPE cathode. Integrating with the anodic ECL emitters, luminol, visual emission of red-blue ratiometric ECL was achieved in BPE array chips. As a sensing probe, Au NRs nanocomposite was assembled on the surface of the cathode and acted as both the quencher of the CdTe QDs ECL and the promoter of the luminol ECL. After incubated with PSA, the Au NRs nanocomposite was peeled off from the Electrode surface due to the specific recognition between PSA and aptamer. Consequently, the cathode ECL partly recovered and the anode ECL turned off. By measuring the ratio of visual ECL intensity at two poles of BPE, sensitive detection of PSA was achieved with a linear range from 1.0 ng/mL to 1.0 μg/mL and detection limit of 0.5 ng/mL (S/N=3). This strategy combining the BPE-ECL and visual ratiometry provided an accurate and intrinsic way for the sensing of PSA and showed good perspective in the clinical diagnosis.

  • Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor.
    Analytical chemistry, 2017
    Co-Authors: Yin-zhu Wang, Wei Zhao, Qi-yuan Guan, Hong-yuan Chen
    Abstract:

    We report a multicolor ECL device based on closed Bipolar Electrode (BPE) for the visualized sensing of prostate-specific antigen (PSA) in human blood serum. As the emission color of concomitant electrochemiluminophores is potential resolved, similar to a three-Electrode system, selective excitation of ECL could be achieved by tuning the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, multicolor ECL emission of [Ru(bpy)3]2+ and [Ir(ppy)3] mixture using tripropylamine (TPrA) as the co-reactant was observed at the anode and the principle was elaborated. The concept was utilized to the quantification of clinical biomarkers with the color variation. A PSA concentration dependent silver bridge was constructed in the gap of the BPEs as an electric conductivity modulator. On the basis of a multicolor BPE-ECL device, the cutoff values (4.0 and 10.0 ng/mL) of human PSA could be recognized with naked eyes by the green–yellow–red ECL emission changing. As the first multicolor ECL dev...

  • temporal sensing platform based on Bipolar Electrode for the ultrasensitive detection of cancer cells
    Analytical Chemistry, 2016
    Co-Authors: Haiwei Shi, Zhen Liu, Wei Zhao, Xicheng Liu, Hong-yuan Chen
    Abstract:

    We report a Bipolar Electrode (BPE) sensing platform for the temporal detection of cancer cells. Combining the advantages of anodic dissolution and electrochemiluminescence (ECL), this strategy shows an ultralow detection limit down to 5 cells/cm2. At the anode working as the reporting pole, Au NPs were assembled through DNA double strand, which served as both catalyzer for the ECL reaction of luminol/H2O2 and seeds for the chemical reduction of Ag, the anodic dissolution probe. The duration of Ag layer dissolution was positively correlated with the amount of Ag but negatively related to the controlled potential and the conductivity of the circuit. Therefore, it was possible to amplify a slight conductivity change through tuning the other two factors. As the formation of Ag@Au completely quenched the ECL emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Through monitoring the ECL recovery time before and after the incubation of cells on the cathode, a few number of...

  • visual color switch electrochemiluminescence biosensing of cancer cell based on multichannel Bipolar Electrode chip
    Analytical Chemistry, 2016
    Co-Authors: Huai-rong Zhang, Yin-zhu Wang, Wei Zhao, Hong-yuan Chen
    Abstract:

    In this work, we developed a visual electrochemiluminescence (ECL) sensing platform based on a dual-Bipolar Electrode (D-BPE) array chip. The chip was composed of two arrays of BPEs and three separated arrays of reservoirs filled with buffer, Ru(bpy)3(2+)-TPrA and luminol solutions, respectively. Both BPEs served as ECL reporting platforms. By applying 6.0 V voltage, an array of orange electrochemiluminescence (ECL) signals belonging to Ru(bpy)3(2+) turned on. After adding DNAzyme and H2O2 in Ru(bpy)3(2+) and luminol reservoirs, the orange Ru(bpy)3(2+) signals decreased until vanished due to the quenching effect; meanwhile, a new array of blue ECL signals turned on because of the luminol-H2O2 ECL reaction. The designed D-BPE owns superior properties compared with the three-Electrode system benefiting from the quantitative relation of Bipolar systems, which greatly enhanced the ECL detection sensitivity. Meanwhile, the visual color-switch ECL and the ratiometric detecting principle made the results easier to evaluate and more accurate. Quantitative detection of HL-60 cancer cells from 320 cells/mL to 2.5 × 10(5) cells/mL with two linear ranges was achieved. The detection limit was down to 80 cells/mL. Finally, this D-BPE chip could distinguish the tumor cells from normal cells and provide a prospect for cancer diagnosis in a high-throughput, visual way.

Taek Dong Chung - One of the best experts on this subject based on the ideXlab platform.

  • Nanoporous ITO implemented Bipolar Electrode sensor for enhanced electrochemiluminescence
    Electrochimica Acta, 2019
    Co-Authors: Minjee Seo, Song Yi Yeon, Jeongse Yun, Taek Dong Chung
    Abstract:

    Abstract We herein achieved a novel indium-tin oxide (ITO) Bipolar Electrode (BPE) based sensing system by the introduction of nanoporous ITO on the cathode. The implementation of the nanoporous ITO layer in the BPE greatly boosted the ECL signal compared to the planar ITO BPE, enabling efficient detection of H2O2 even under mild operating voltage. The calibration curves of ECLs in H2O2 sensing with BPEs of various nanopore thicknesses exhibited enhancement of LODs and sensitivities with a thicker nanoporous ITO layer. We speculate that the morphology of the nanopores led to confinement of the analytes, increasing the interaction with the Electrode surface, resulting in amplified ECL signals. We believe that the nanoporous BPEs are adequately suited for the extended application as a compartment in portable, in situ sensing systems, due to their low power requirements and ease of detection through the naked eye.

  • miniaturized reverse electrodialysis powered biosensor using electrochemiluminescence on Bipolar Electrode
    Analytical Chemistry, 2018
    Co-Authors: Seol Hee Baek, Seung-ryong Kwon, Song Yi Yeon, Sunheui Yoon, Chung Mu Kang, Taek Dong Chung
    Abstract:

    We suggest an electrochemiluminescence (ECL)-sensing platform driven by ecofriendly, disposable, and miniaturized reverse electrodialysis (RED) patches as an electric power source. The flexible RED patches composed of ion-exchange membranes (IEMs) can produce voltage required for ECL sensing by simply choosing the appropriate number of IEMs and the ratio of salt concentrations. We integrate the RED patch with a Bipolar Electrode on the microfluidic chip to demonstrate the proof-of-concept, i.e., glucose detection in the range of 0.5–10 mM by observing ECL emissions with naked eyes. The miniaturized RED-powered biosensing system is widely applicable for electrochemical-sensing platforms. This is expected to be a solution for practical availability of battery-free electrochemical sensors for disease diagnosis in developing countries.

  • miniaturized reverse electrodialysis powered biosensor using electrochemiluminescence on Bipolar Electrode
    Analytical Chemistry, 2018
    Co-Authors: Seol Hee Baek, Seung-ryong Kwon, Song Yi Yeon, Sunheui Yoon, Chung Mu Kang, Taek Dong Chung
    Abstract:

    We suggest an electrochemiluminescence (ECL)-sensing platform driven by ecofriendly, disposable, and miniaturized reverse electrodialysis (RED) patches as an electric power source. The flexible RED patches composed of ion-exchange membranes (IEMs) can produce voltage required for ECL sensing by simply choosing the appropriate number of IEMs and the ratio of salt concentrations. We integrate the RED patch with a Bipolar Electrode on the microfluidic chip to demonstrate the proof-of-concept, i.e., glucose detection in the range of 0.5–10 mM by observing ECL emissions with naked eyes. The miniaturized RED-powered biosensing system is widely applicable for electrochemical-sensing platforms. This is expected to be a solution for practical availability of battery-free electrochemical sensors for disease diagnosis in developing countries.

  • Miniaturized Reverse Electrodialysis-Powered Biosensor Using Electrochemiluminescence on Bipolar Electrode
    2018
    Co-Authors: Seol Baek, Seung-ryong Kwon, Song Yi Yeon, Sunheui Yoon, Chung Mu Kang, Seok Hee Han, Dahye Lee, Taek Dong Chung
    Abstract:

    We suggest an electrochemiluminescence (ECL)-sensing platform driven by ecofriendly, disposable, and miniaturized reverse electrodialysis (RED) patches as an electric power source. The flexible RED patches composed of ion-exchange membranes (IEMs) can produce voltage required for ECL sensing by simply choosing the appropriate number of IEMs and the ratio of salt concentrations. We integrate the RED patch with a Bipolar Electrode on the microfluidic chip to demonstrate the proof-of-concept, i.e., glucose detection in the range of 0.5–10 mM by observing ECL emissions with naked eyes. The miniaturized RED-powered biosensing system is widely applicable for electrochemical-sensing platforms. This is expected to be a solution for practical availability of battery-free electrochemical sensors for disease diagnosis in developing countries

Richard M Crooks - One of the best experts on this subject based on the ideXlab platform.

  • Dual-channel Bipolar Electrode focusing: simultaneous separation and enrichment of both anions and cations.
    Lab on a chip, 2012
    Co-Authors: Kyle N Knust, Robbyn K. Anand, Eoin Sheridan, Richard M Crooks
    Abstract:

    In this paper we show that a microelectrochemical cell comprising two parallel microchannels spanned by a single Bipolar Electrode can be used to simultaneously enrich and separate both anions and cations within a single microchannel. This is possible because reduction and oxidation of water at the cathodic and anodic poles of the Bipolar Electrode, respectively, lead to ion depletion zones. Specifically, TrisH+ is neutralized by OH− at the cathodic pole, while acetate buffer is neutralized by H+ at the anodic pole. This action creates a local electric field gradient having both positive and negative components, and hence positive and negative ions follow their respective field gradients leading to separation. In the presence of an opposing counter-flow (pressure driven flow in this case), enrichment also occurs. In addition to separation and enrichment in a single channel, it is also possible to simultaneously enrich cations in one microchannel and anions in the other. Enrichment is achieved by controlling experimental parameters, including the type of buffer and the direction and magnitude of the opposing counter-flow.

  • Enrichment of cations via Bipolar Electrode focusing.
    Analytical chemistry, 2012
    Co-Authors: Eoin Sheridan, Dzmitry Hlushkou, Ulrich Tallarek, Kyle N Knust, Richard M Crooks
    Abstract:

    We have previously demonstrated up to 5 × 105-fold enrichment of anionic analytes in a microchannel using a technique called Bipolar Electrode focusing (BEF). Here, we demonstrate that BEF can also be used to enrich a cationic fluorescent tracer. The important point is that chemical modification of the microchannel walls enables reversal of the electroosmotic flow (EOF), enabling cations, instead of anions, to be enriched via an electric field gradient focusing mechanism. Reversal of the EOF has significant consequences on the formation and shape of the region of the buffer solution depleted of charge carriers (depletion zone). Electric field measurements and numerical simulations are used to elucidate the factors influencing the depletion zone. This information is used to understand and control the location and shape of the depletion zone, which in turn influences the stability and concentration of the enriched band.

  • two channel microelectrochemical Bipolar Electrode sensor array
    Analyst, 2012
    Co-Authors: Young Yong Chang, Francois Mavre, Kwokfa Chow, Joh A Crooks, Richard M Crooks
    Abstract:

    We report a two-channel microelectrochemical sensor that communicates between separate sensing and reporting microchannels via one or more Bipolar Electrodes (BPEs). Depending on the contents of each microchannel and the voltage applied across the BPE, faradaic reactions may be activated simultaneously in both channels. As presently configured, one end of the BPE is designated as the sensing pole and the other as the reporting pole. When the sensing pole is activated by a target, electrogenerated chemiluminescence (ECL) is emitted at the reporting pole. Compared to previously reported single-channel BPE sensors, the key advantage of the multichannel architecture reported here is physical separation of the ECL reporting cocktail and the solution containing the target. This prevents chemical interference between the two channels.

  • Bipolar Electrode depletion membraneless filtration of charged species using an electrogenerated electric field gradient
    Analyst, 2011
    Co-Authors: Eoin Sheridan, Kyle N Knust, Richard M Crooks
    Abstract:

    We report a method for removing ions from aqueous solutions without the use of a membrane. The approach, which we call Bipolar Electrode depletion (BED), is based on the formation of an asymmetric electric field profile in a microchannel containing a Bipolar Electrode (BPE). The asymmetric field arises from local increases in conductivity caused by faradaic reactions at the BPE. We show how the asymmetric field can be used to deplete anions from a microchannel via a combination of electrophoresis and electroosmosis. We also apply this approach to filter an anionic species from a mixture of charged and neutral species being transported through a microchannel via electroosmosis. This technique could be utilized for desalination or filtration of any species possessing a net charge (e.g. heavy-metals, bacteria, proteins, or functionalized-nanoparticles).

  • Label-free electrochemical monitoring of concentration enrichment during Bipolar Electrode focusing.
    Analytical chemistry, 2011
    Co-Authors: Eoin Sheridan, Robbyn K. Anand, Dzmitry Hlushkou, Ulrich Tallarek, Derek R. Laws, Richard M Crooks
    Abstract:

    We show that a label-free electrochemical method can be used to monitor the position of an enriched analyte band during Bipolar Electrode focusing in a microfluidic device. The method relies on formation of a depleted buffer cation region, which is responsible for concentration enrichment of the charged analyte. However, this depletion region also leads to an increase in the local electric field in the solution near a Bipolar Electrode (BPE), and this in turn results in enhanced faradaic reactions (oxidation and reduction of water) at the BPE. Therefore, it is possible to detect the presence of the concentrated analyte band by measuring the current passing through the BPE used for concentration enrichment, or the concentrated band can be detected at a secondary BPE dedicated to that purpose. Both experiments and simulations are presented that fully elucidate the underlying phenomenon responsible for these observations.

Erkang Wang - One of the best experts on this subject based on the ideXlab platform.

  • Dual-electrochromic Bipolar Electrode-based universal platform for the construction of various visual advanced logic devices
    NPG Asia Materials, 2017
    Co-Authors: Qingfeng Zhai, Daoqing Fan, Xiaowei Zhang, Erkang Wang
    Abstract:

    A universal logic platform based on dual-electrochromic Bipolar Electrodes (BPEs) for the operation of various visual advanced logic devices was designed and constructed for the first time. Two BPEs separated by three reaction channels filled with different reaction solutions constituted the closed BPE system and were used as the initial state. Under different input combinations, the electrochemical oxidation of 2, 2′ -azinobis (3-ethylbenzthiazoline-6-sulfonic acid) and the Electrodeposition of Prussian blue occurred at different poles simultaneously and triggered rapid color changes that could be easily visualized by the naked eye. By defining the color changes at the two specific poles as outputs, visual advanced logic devices, including an encoder, a decoder, a demultiplexer, a keypad lock and a three-input concatenated logic circuit with two outputs, were successfully constructed. A simple electrochemical device that performs advanced logic operations has been developed by scientists in China. A Bipolar Electrode is a conducting material in an ionic liquid. A voltage applied across the liquid initiates electrochemical reactions that create positive and negative charges at the opposite ends of the Electrode. Thus, electrical current flows through the Bipolar Electrode even without a current in the surrounding liquid. Now, Erkang Wang from the Changchun Institute of Applied Chemistry and colleagues have created a Bipolar Electrode that, unlike previous devices, can produce an optical output visible to the naked eye. Their platform comprises two Bipolar Electrodes separated by three electrochemical reaction channels. The researchers demonstrated its potential in various logic devices including an encoder, a decoder, a demultiplexer and a keypad lock. A universal dual-electrochromic platform that could operate various advanced logic devices whose outputs visualized by the naked eye was constructed for the first time by introducing the electrochemical oxidation of 2, 2′ -azinobis (3-ethylbenzthiazoline-6-sulfonic acid) and Electrodeposition of Prussian blue into one closed Bipolar Electrode system.

  • Bipolar Electrode based reversible fluorescence switch using prussian blue au nanoclusters nanocomposite film
    Analytical Chemistry, 2017
    Co-Authors: Huanhuan Xing, Qingfeng Zhai, Xiaowei Zhang, Erkang Wang
    Abstract:

    A highly efficient fluorescence switch system based on a closed Bipolar Electrode (C-BPE) system was proposed for the first time. Here, Au nanoclusters (Au NCs) were premodified on one pole of the BPE and acted as the fluorescent donor. On the basis of the spectral overlap between the absorbance of electrochromic material-Prussian blue (PB) and the fluorescence spectrum of Au NCs, fluorescence quenching (“off” state) induced by the inner filter effect was observed. Due to the electrochemical reversible redox reaction between PB and Prussian white, switching the polarity of driving voltage could easily achieve the fluorescence recovery of the Au NCs, corresponding to the “on” state. Through the reasonable design of C-BPE and optimization of driving voltage, the on–off ratio of the integrated fluorescence switch was up to 2.7 and a good fatigue resistance while performing 10 on–off cycles was obtained owing to the good stability of Au NCs and the reversible redox feature of PB. The introduction of BPE made ...

  • a nanoscale multichannel closed Bipolar Electrode array for electrochemiluminescence sensing platform
    Analytical Chemistry, 2016
    Co-Authors: Qingfeng Zhai, Xiaowei Zhang, Yanchao Han, Junfeng Zhai, Erkang Wang
    Abstract:

    In this work, we report a nanoscale multichannel closed Bipolar Electrode (BPE) array based on the poly(ethylene terephthalate) (PET) membrane for the first time. With our design, oxidants, coreactants, quenchers, and even biomarkers can be detected in a Ru(bpy)32+/TPA (tripropylamine) electrochemiluminescence (ECL) system. The multichannel PET membrane was etched according to our desire by NaOH, and then Au nanofibers were decorated in the inner region of the channel as a BPE array. Using ECL as a signal readout, a series of targets including TPA, Ru(bpy)32+, dopamine, H2O2, alpha-fetoprotein (AFP), and carcino-embryonic antigen (CEA) can be detected with this device. The practical application of the proposed multichannel closed BPE array was verified in the detection of AFP and CEA in human serum with satisfying results. This kind of nanoscale device holds promising potential for multianalysis. More importantly, as the PET membrane used in this device can be etched with a desirable diameter (nano- to mi...

Wei Zhao - One of the best experts on this subject based on the ideXlab platform.

  • Visual electrochemiluminescence ratiometry on Bipolar Electrode for bioanalysis.
    Biosensors & bioelectronics, 2017
    Co-Authors: Wei Zhao, Hong-yuan Chen
    Abstract:

    Abstract In this work, we developed a visual ECL ratiometry on a closed Bipolar Electrode (BPE) for the detection of prostate specific antigen (PSA), prostate cancer biomarker. High efficient CdTe QDs was synthesized which emitted visualized red light at BPE cathode. Integrating with the anodic ECL emitters, luminol, visual emission of red-blue ratiometric ECL was achieved in BPE array chips. As a sensing probe, Au NRs nanocomposite was assembled on the surface of the cathode and acted as both the quencher of the CdTe QDs ECL and the promoter of the luminol ECL. After incubated with PSA, the Au NRs nanocomposite was peeled off from the Electrode surface due to the specific recognition between PSA and aptamer. Consequently, the cathode ECL partly recovered and the anode ECL turned off. By measuring the ratio of visual ECL intensity at two poles of BPE, sensitive detection of PSA was achieved with a linear range from 1.0 ng/mL to 1.0 μg/mL and detection limit of 0.5 ng/mL (S/N=3). This strategy combining the BPE-ECL and visual ratiometry provided an accurate and intrinsic way for the sensing of PSA and showed good perspective in the clinical diagnosis.

  • Bipolar Electrode Based Multicolor Electrochemiluminescence Biosensor.
    Analytical chemistry, 2017
    Co-Authors: Yin-zhu Wang, Wei Zhao, Qi-yuan Guan, Hong-yuan Chen
    Abstract:

    We report a multicolor ECL device based on closed Bipolar Electrode (BPE) for the visualized sensing of prostate-specific antigen (PSA) in human blood serum. As the emission color of concomitant electrochemiluminophores is potential resolved, similar to a three-Electrode system, selective excitation of ECL could be achieved by tuning the interfacial potential at the poles of BPE. Via modulating the resistance of BPE, multicolor ECL emission of [Ru(bpy)3]2+ and [Ir(ppy)3] mixture using tripropylamine (TPrA) as the co-reactant was observed at the anode and the principle was elaborated. The concept was utilized to the quantification of clinical biomarkers with the color variation. A PSA concentration dependent silver bridge was constructed in the gap of the BPEs as an electric conductivity modulator. On the basis of a multicolor BPE-ECL device, the cutoff values (4.0 and 10.0 ng/mL) of human PSA could be recognized with naked eyes by the green–yellow–red ECL emission changing. As the first multicolor ECL dev...

  • temporal sensing platform based on Bipolar Electrode for the ultrasensitive detection of cancer cells
    Analytical Chemistry, 2016
    Co-Authors: Haiwei Shi, Zhen Liu, Wei Zhao, Xicheng Liu, Hong-yuan Chen
    Abstract:

    We report a Bipolar Electrode (BPE) sensing platform for the temporal detection of cancer cells. Combining the advantages of anodic dissolution and electrochemiluminescence (ECL), this strategy shows an ultralow detection limit down to 5 cells/cm2. At the anode working as the reporting pole, Au NPs were assembled through DNA double strand, which served as both catalyzer for the ECL reaction of luminol/H2O2 and seeds for the chemical reduction of Ag, the anodic dissolution probe. The duration of Ag layer dissolution was positively correlated with the amount of Ag but negatively related to the controlled potential and the conductivity of the circuit. Therefore, it was possible to amplify a slight conductivity change through tuning the other two factors. As the formation of Ag@Au completely quenched the ECL emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Through monitoring the ECL recovery time before and after the incubation of cells on the cathode, a few number of...

  • visual color switch electrochemiluminescence biosensing of cancer cell based on multichannel Bipolar Electrode chip
    Analytical Chemistry, 2016
    Co-Authors: Huai-rong Zhang, Yin-zhu Wang, Wei Zhao, Hong-yuan Chen
    Abstract:

    In this work, we developed a visual electrochemiluminescence (ECL) sensing platform based on a dual-Bipolar Electrode (D-BPE) array chip. The chip was composed of two arrays of BPEs and three separated arrays of reservoirs filled with buffer, Ru(bpy)3(2+)-TPrA and luminol solutions, respectively. Both BPEs served as ECL reporting platforms. By applying 6.0 V voltage, an array of orange electrochemiluminescence (ECL) signals belonging to Ru(bpy)3(2+) turned on. After adding DNAzyme and H2O2 in Ru(bpy)3(2+) and luminol reservoirs, the orange Ru(bpy)3(2+) signals decreased until vanished due to the quenching effect; meanwhile, a new array of blue ECL signals turned on because of the luminol-H2O2 ECL reaction. The designed D-BPE owns superior properties compared with the three-Electrode system benefiting from the quantitative relation of Bipolar systems, which greatly enhanced the ECL detection sensitivity. Meanwhile, the visual color-switch ECL and the ratiometric detecting principle made the results easier to evaluate and more accurate. Quantitative detection of HL-60 cancer cells from 320 cells/mL to 2.5 × 10(5) cells/mL with two linear ranges was achieved. The detection limit was down to 80 cells/mL. Finally, this D-BPE chip could distinguish the tumor cells from normal cells and provide a prospect for cancer diagnosis in a high-throughput, visual way.