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Dosun Lim - One of the best experts on this subject based on the ideXlab platform.
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effects of Black Raspberry on lipid profiles and vascular endothelial function in patients with metabolic syndrome
Phytotherapy Research, 2014Co-Authors: Han Saem Jeong, Ji-wung Kwon, Soon Jun Hong, Tae Bum Lee, Jong Tae Jeong, Hyung Joon Joo, Jae Hyoung Park, Chul Min Ahn, Dosun LimAbstract:Black Raspberry (Rubus occidentalis) has been known for its anti-inflammatory and anti-oxidant effects. However, short-term effects of Black Raspberry on lipid profiles and vascular endothelial function have not been investigated in patients with metabolic syndrome. Patients with metabolic syndrome (n = 77) were prospectively randomized into a group with Black Raspberry (n = 39, 750 mg/day) and a placebo group (n = 38) during a 12-week follow-up. Lipid profiles, brachial artery flow-mediated dilatation (baFMD), and inflammatory cytokines such as IL-6, TNF-α, C-reactive protein, adiponectin, sICAM-1, and sVCAM-1 were measured at the baseline and at the 12-week follow-up. Decreases from the baseline in the total cholesterol level (-22.8 ± 30.4 mg/dL vs. -1.9 ± 31.8 mg/dL, p < 0.05, respectively) and total cholesterol/HDL ratio (-0.31 ± 0.64 vs. 0.07 ± 0.58, p < 0.05, respectively) were significantly greater in the group with Black Raspberry than in the placebo group. Increases in baFMD at the 12-week follow-up were significantly greater in the group with Black Raspberry than in the placebo group (0.33 ± 0.44 mm vs. 0.10 ± 0.35 mm, p < 0.05, respectively). Decreases from the baseline in IL-6 (-0.4 ± 1.5 pg/mL vs. -0.1 ± 1.0 pg/mL, p < 0.05, respectively) and TNF-α (-2.9 ± 4.7 pg/mL vs. 0.1 ± 3.6 pg/mL, p < 0.05, respectively) were significantly greater in the group with Black Raspberry. The use of Black Raspberry significantly decreased serum total cholesterol level and inflammatory cytokines, thereby improving vascular endothelial function in patients with metabolic syndrome during the 12-week follow-up.
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Effects of Black Raspberry on lipid profiles and vascular endothelial function in patients with metabolic syndrome.
Phytotherapy research : PTR, 2014Co-Authors: Han Saem Jeong, Ji-wung Kwon, Soon Jun Hong, Tae Bum Lee, Jong Tae Jeong, Hyung Joon Joo, Jae Hyoung Park, Chul Min Ahn, Dosun LimAbstract:Black Raspberry (Rubus occidentalis) has been known for its anti-inflammatory and anti-oxidant effects. However, short-term effects of Black Raspberry on lipid profiles and vascular endothelial function have not been investigated in patients with metabolic syndrome. Patients with metabolic syndrome (n = 77) were prospectively randomized into a group with Black Raspberry (n = 39, 750 mg/day) and a placebo group (n = 38) during a 12-week follow-up. Lipid profiles, brachial artery flow-mediated dilatation (baFMD), and inflammatory cytokines such as IL-6, TNF-α, C-reactive protein, adiponectin, sICAM-1, and sVCAM-1 were measured at the baseline and at the 12-week follow-up. Decreases from the baseline in the total cholesterol level (-22.8 ± 30.4 mg/dL vs. -1.9 ± 31.8 mg/dL, p
Chad E. Finn - One of the best experts on this subject based on the ideXlab platform.
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A near complete, chromosome-scale assembly of the Black Raspberry (Rubus occidentalis) genome
GigaScience, 2018Co-Authors: Robert Vanburen, Jill M. Bushakra, Kelly J. Vining, Michael Dossett, Ching Man Wai, Marivi Colle, Jie Wang, Shawn Sullivan, Ivan Liachko, Chad E. FinnAbstract:Background The fragmented nature of most draft plant genomes has hindered downstream gene discovery, trait mapping for breeding, and other functional genomics applications. There is a pressing need to improve or finish draft plant genome assemblies. Findings Here, we present a chromosome-scale assembly of the Black Raspberry genome using single-molecule real-time Pacific Biosciences sequencing and high-throughput chromatin conformation capture (Hi-C) genome scaffolding. The updated V3 assembly has a contig N50 of 5.1 Mb, representing an ∼200-fold improvement over the previous Illumina-based version. Each of the 235 contigs was anchored and oriented into seven chromosomes, correcting several major misassemblies. Black Raspberry V3 contains 47 Mb of new sequences including large pericentromeric regions and thousands of previously unannotated protein-coding genes. Among the new genes are hundreds of expanded tandem gene arrays that were collapsed in the Illumina-based assembly. Detailed comparative genomics with the high-quality V4 woodland strawberry genome (Fragaria vesca) revealed near-perfect 1:1 synteny with dramatic divergence in tandem gene array composition. Lineage-specific tandem gene arrays in Black Raspberry are related to agronomic traits such as disease resistance and secondary metabolite biosynthesis. Conclusions The improved resolution of tandem gene arrays highlights the need to reassemble these highly complex and biologically important regions in draft plant genomes. The updated, high-quality Black Raspberry reference genome will be useful for comparative genomics across the horticulturally important Rosaceae family and enable the development of marker assisted breeding in Rubus.
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Characterization of aphid resistance loci in Black Raspberry (Rubus occidentalis L.)
Molecular Breeding, 2018Co-Authors: Jill M. Bushakra, Kelly J. Vining, Robert Vanburen, Michael Dossett, Todd C. Mockler, Chad E. Finn, Katherine A. Carter, Douglas W. Bryant, Nahla V. BassilAbstract:Viruses vectored by the aphid Amphorophora agathonica cause decline in Black Raspberry plant health resulting in a shortened life and poor fruit quality of the infected plantings. New aphid resistant cultivars could increase the longevity of plantings providing growers and processors with consistent fruit production. Recent exploration of the native range of Black Raspberry identified three sources of aphid resistance: Ag _ 4 from Ontario (ON), Canada, Ag _ 5 from Maine (ME), and a third source from Michigan (MI) with no formal designation. The objectives of this study were to assess segregation of these three sources of aphid resistance in populations with single and combined sources and develop markers that can identify each source of resistance. A genetic linkage map constructed for ORUS 4305 placed the ON aphid resistance locus on Rubus linkage group (RLG) 6. Segregation ratios in populations with single and combined sources, and linkage mapping in two populations (ORUS 4304 and ORUS 4812) segregating for the Ag _ 5 and MI sources, respectively, indicated that these three sources of resistance are each conferred by single dominant genes/alleles that are linked on RLG6. Confirmation of marker association in 16 validation populations identified four markers that could be used to predict resistance; however, none could distinguish between the ON and MI sources. These four markers may be useful for screening populations to enrich the field-planted progeny for aphid resistance. Fine mapping of the resistance loci is needed to develop functional markers at each of the resistance loci to enable pyramiding and durable aphid resistance.
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Chromosome-scale scaffolding of the Black Raspberry ( Rubus occidentalis L.) genome based on chromatin interaction data
Horticulture research, 2018Co-Authors: Rubina Jibran, Jill M. Bushakra, Kelly J. Vining, Patrick P. Edger, Nahla V. Bassil, Michael Dossett, Chad E. Finn, Shawn Sullivan, Helge Dzierzon, Robert VanburenAbstract:Black Raspberry (Rubus occidentalis L.) is a niche fruit crop valued for its flavor and potential health benefits. The improvement of fruit and cane characteristics via molecular breeding technologies has been hindered by the lack of a high-quality reference genome. The recently released draft genome for Black Raspberry (ORUS 4115-3) lacks assembly of scaffolds to chromosome scale. We used high-throughput chromatin conformation capture (Hi-C) and Proximity-Guided Assembly (PGA) to cluster and order 9650 out of 11,936 contigs of this draft genome assembly into seven pseudo-chromosomes. The seven pseudo-chromosomes cover ~97.2% of the total contig length (~223.8 Mb). Locating existing genetic markers on the physical map resolved multiple discrepancies in marker order on the genetic map. Centromeric regions were inferred from recombination frequencies of genetic markers, alignment of 303 bp centromeric sequence with the PGA, and heat map showing the physical contact matrix over the entire genome. We demonstrate a high degree of synteny between each of the seven chromosomes of Black Raspberry and a high-quality reference genome for strawberry (Fragaria vesca L.) assembled using only PacBio long-read sequences. We conclude that PGA is a cost-effective and rapid method of generating chromosome-scale assemblies from Illumina short-read sequencing data. Assembly of a high-quality reference genome for the Black Raspberry plant will inform future crop improvements. Sequencing the genomes of widely-grown, profitable fruit crops can help researchers identify the DNA markers linked to desirable traits, potentially improving crop health and yields. David Chagne at the New Zealand Institute for Plant and Food Research Limited and co-workers combined new techniques to significantly improve on an existing genome for the Black Raspberry (Rubus occidentalis L.). The researchers used Hi-C analysis to create a map identifying the interactions between chromatin fragments – macromolecules made from DNA, proteins and RNA – and the three-dimensional structure of chromosomes inside the cell nucleus. Then, they used a bioinformatics assembly method to construct the genome from this data. The new genome showed high accuracy when compared to another genome from the same family.
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Chromosome-scale scaffolding of the Black Raspberry (Rubus occidentalis L.) genome based on chromatin interaction data
Nature Publishing Group, 2018Co-Authors: Rubina Jibran, Jill M. Bushakra, Kelly J. Vining, Patrick P. Edger, Michael Dossett, Chad E. Finn, Shawn Sullivan, Helge Dzierzon, Nahla Bassil, Robert VanburenAbstract:Plant genomics: Improved genome for the Black Raspberry Assembly of a high-quality reference genome for the Black Raspberry plant will inform future crop improvements. Sequencing the genomes of widely-grown, profitable fruit crops can help researchers identify the DNA markers linked to desirable traits, potentially improving crop health and yields. David Chagné at the New Zealand Institute for Plant and Food Research Limited and co-workers combined new techniques to significantly improve on an existing genome for the Black Raspberry (Rubus occidentalis L.). The researchers used Hi-C analysis to create a map identifying the interactions between chromatin fragments – macromolecules made from DNA, proteins and RNA – and the three-dimensional structure of chromosomes inside the cell nucleus. Then, they used a bioinformatics assembly method to construct the genome from this data. The new genome showed high accuracy when compared to another genome from the same family
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Performance and phenology of wild Black Raspberry (Rubus occidentalis L.) germplasm in a common garden
Genetic Resources and Crop Evolution, 2016Co-Authors: Michael Dossett, Chad E. FinnAbstract:A lack of genetic diversity in cultivated Black Raspberry ( Rubus occidentalis L.) germplasm has been widely recognized as a major factor limiting progress towards breeding improved cultivars. Despite this, little effort has been made since the early twentieth century to systematically collect and evaluate wild Black Raspberry for germplasm improvement. In recent years, there has been renewed interest in Black Raspberry breeding to replace existing cultivars that lack durability and disease resistance. We planted seedlings from 109 wild Black Raspberry populations, representing 24 US states and two Canadian provinces, in the field in replicated trial plots in Corvallis, Oregon (USA), to evaluate their performance. These populations showed wide variation in morphology, architecture, fruiting season, vigor, and apparent field tolerance to Verticillium wilt. For nearly every trait examined, wild Black Raspberry germplasm exhibited a range of variation beyond existing cultivars, and showed great potential for use in future breeding. While most populations were fairly uniform phenotypically, segregation for fruit gloss and possible tolerance to Verticillium wilt was noted in a few, indicating the possibility of simple inheritance of these traits. A few populations with unusual morphology, such as spinelessness or flower abnormalities, were identified, as were populations that flowered on first year canes and produced fall fruit. Populations from the southern edge of the range appear to be particularly well adapted to western Oregon, with vigorous upright growth, strong canes, and some with a low incidence of Verticillium wilt. This germplasm will be of great value to breeders interested in developing improved Black Raspberry cultivars.
Ji-wung Kwon - One of the best experts on this subject based on the ideXlab platform.
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Immune Enhancing Effects of Aqueous Extracted Black Raspberry in Diet-induced Hyperlipidemia Mice
Journal of Immunology, 2016Co-Authors: Youngjoo Lee, Jiyeon Kim, Sungwon Lee, Heetae Lee, Hyunseok Kong, Youngcheon Song, Hye Ran Choi, Daekeun Shin, Ji-wung KwonAbstract:As obese population has increased today, metabolic diseases accompanied with obesity have been issued as well. Hyperlipidemia, one of those metabolic abnormalities, is commonly diagnosed with obesity. It is also associated with inflammation and immune dysfunctions, which can be led to global immune decline as the host ages. In this study, the potential effects of Black Raspberry on the diet-induced hyperlipidemia were examined in terms of immunology. We first induced hyperlipidemia in mice by feeding with 45kcal high fat diet (HFD) for 23 weeks and the progress of disease was traced by blood lipid profiling. Oral administration of Black Raspberry then proceeded for 16 weeks. As a result, the lymphocyte subset populations such as CD4 + , CD8 + and CD11c + were quantitatively restored by Black Raspberry treatment. The proliferation and cytokine production of lymphocytes were also normalized in Black Raspberry treated mice. Regarding specific immunity, oral administration of Black Raspberry significantly restored both cellular and humoral immune function as determined by in vivo cytotoxic T lymphocytes (CTLs) assay and specific immunoglobulin G (IgG) production level. Lastly the lipid indices including total cholesterol and HDL were considerably improved by Black Raspberry treatment, indicating the therapeutic effect of Black Raspberry on hyperlipidemia. That is, not only practical improvement in hyperlipidemia, but also the immune-enhancing effect of Black Raspberry was newly elucidated with quantitative and qualitative evidences.
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Black Raspberry extract increased circulating endothelial progenitor cells and improved arterial stiffness in patients with metabolic syndrome a randomized controlled trial
Journal of Medicinal Food, 2016Co-Authors: Han Saem Jeong, Soon Jun Hong, Tae Bum Lee, Sohyeon Kim, Seung Cheol Choi, Ji Hyun Choi, Jong Ho Kim, Chi Yeon Park, Jae Young Cho, Ji-wung KwonAbstract:Administration of Black Raspberry (Rubus occidentalis) is known to improve vascular endothelial function in patients at a high risk for cardiovascular (CV) disease. We investigated short-term effects of Black Raspberry on circulating endothelial progenitor cells (EPCs) and arterial stiffness in patients with metabolic syndrome. Patients with metabolic syndrome (n = 51) were prospectively randomized into the Black Raspberry group (n = 26, 750 mg/day) and placebo group (n = 25) during the 12-week follow-up. Central blood pressure, augmentation index, and EPCs, such as CD34/KDR(+), CD34/CD117(+), and CD34/CD133(+), were measured at baseline and at 12-week follow-up. Radial augmentation indexes were significantly decreased in the Black Raspberry group compared to the placebo group (-5% ± 10% vs. 3% ± 14%, P < .05). CD34/CD133(+) cells at 12-week follow-up were significantly higher in the Black Raspberry group compared to the placebo group (19 ± 109/μL vs. -28 ± 57/μL, P < .05). Decreases from the baseline in interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were significantly greater in the Black Raspberry group compared to the placebo group (-0.5 ± 1.4 pg/mL vs. -0.1 ± 1.1 pg/mL, P < .05 and -5.4 ± 4.5 pg/mL vs. -0.8 ± 4.0 pg/mL, P < .05, respectively). Increases from the baseline in adiponectin levels (2.9 ± 2.1 μg/mL vs. -0.2 ± 2.5 μg/mL, P < .05) were significant in the Black Raspberry group. The use of Black Raspberry significantly lowered the augmentation index and increased circulating EPCs, thereby improving CV risks in patients with metabolic syndrome during the 12-week follow-up.
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Black Raspberry Extract Increased Circulating Endothelial Progenitor Cells and Improved Arterial Stiffness in Patients with Metabolic Syndrome: A Randomized Controlled Trial
Journal of medicinal food, 2016Co-Authors: Han Saem Jeong, Soon Jun Hong, Tae Bum Lee, Sohyeon Kim, Seung Cheol Choi, Ji Hyun Choi, Jong Ho Kim, Chi Yeon Park, Jae Young Cho, Ji-wung KwonAbstract:Administration of Black Raspberry (Rubus occidentalis) is known to improve vascular endothelial function in patients at a high risk for cardiovascular (CV) disease. We investigated short-term effects of Black Raspberry on circulating endothelial progenitor cells (EPCs) and arterial stiffness in patients with metabolic syndrome. Patients with metabolic syndrome (n = 51) were prospectively randomized into the Black Raspberry group (n = 26, 750 mg/day) and placebo group (n = 25) during the 12-week follow-up. Central blood pressure, augmentation index, and EPCs, such as CD34/KDR(+), CD34/CD117(+), and CD34/CD133(+), were measured at baseline and at 12-week follow-up. Radial augmentation indexes were significantly decreased in the Black Raspberry group compared to the placebo group (-5% ± 10% vs. 3% ± 14%, P
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effects of Black Raspberry on lipid profiles and vascular endothelial function in patients with metabolic syndrome
Phytotherapy Research, 2014Co-Authors: Han Saem Jeong, Ji-wung Kwon, Soon Jun Hong, Tae Bum Lee, Jong Tae Jeong, Hyung Joon Joo, Jae Hyoung Park, Chul Min Ahn, Dosun LimAbstract:Black Raspberry (Rubus occidentalis) has been known for its anti-inflammatory and anti-oxidant effects. However, short-term effects of Black Raspberry on lipid profiles and vascular endothelial function have not been investigated in patients with metabolic syndrome. Patients with metabolic syndrome (n = 77) were prospectively randomized into a group with Black Raspberry (n = 39, 750 mg/day) and a placebo group (n = 38) during a 12-week follow-up. Lipid profiles, brachial artery flow-mediated dilatation (baFMD), and inflammatory cytokines such as IL-6, TNF-α, C-reactive protein, adiponectin, sICAM-1, and sVCAM-1 were measured at the baseline and at the 12-week follow-up. Decreases from the baseline in the total cholesterol level (-22.8 ± 30.4 mg/dL vs. -1.9 ± 31.8 mg/dL, p < 0.05, respectively) and total cholesterol/HDL ratio (-0.31 ± 0.64 vs. 0.07 ± 0.58, p < 0.05, respectively) were significantly greater in the group with Black Raspberry than in the placebo group. Increases in baFMD at the 12-week follow-up were significantly greater in the group with Black Raspberry than in the placebo group (0.33 ± 0.44 mm vs. 0.10 ± 0.35 mm, p < 0.05, respectively). Decreases from the baseline in IL-6 (-0.4 ± 1.5 pg/mL vs. -0.1 ± 1.0 pg/mL, p < 0.05, respectively) and TNF-α (-2.9 ± 4.7 pg/mL vs. 0.1 ± 3.6 pg/mL, p < 0.05, respectively) were significantly greater in the group with Black Raspberry. The use of Black Raspberry significantly decreased serum total cholesterol level and inflammatory cytokines, thereby improving vascular endothelial function in patients with metabolic syndrome during the 12-week follow-up.
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improvement of cholesterol and blood pressure in fruit leaf and stem extracts from Black Raspberry in vitro
The Korean Journal of Medicinal Crop Science, 2014Co-Authors: Min Jung Lee, Hye Ran Choi, Ji-wung Kwon, Jong Tae Jeong, Su Jung Lee, Junghyun Lee, Kyu Seo Chae, Tae Bum LeeAbstract:We compared effects of fruit, leaf, and stem extracts from Black Raspberry on improvement of cholesterol and blood pressure in HepG2 and HUVEC cells, respectively. Cholesterol secretion was inhibited by water extracts of unripe fruit and stem, but not leaf of Black Raspberry in HepG2 cells. Also, water extracts of unripe fruit, leaf, and stem reduced HMG-CoA reductase activity. Furthermore, nitric oxide production and expression of angiotensin-converting enzyme (ACE) protein were regulated by extracts of fruit, leaf, and stem of Black Raspberry in HUVEC cells. Overall, the rank order according to the improving level of cholesterol and hypertension is as follows: stem > fruit > leaf. In addition, various polyphenol compounds displayed inhibitory effects of HMG-CoA reductase activity and ACE expression. Thus, these data suggested that leaf and stem as wells as fruit of Black Raspberry can be used as useful food resources for reduction of cholesterol and blood pressure.
Jong Tae Jeong - One of the best experts on this subject based on the ideXlab platform.
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effects of Black Raspberry on lipid profiles and vascular endothelial function in patients with metabolic syndrome
Phytotherapy Research, 2014Co-Authors: Han Saem Jeong, Ji-wung Kwon, Soon Jun Hong, Tae Bum Lee, Jong Tae Jeong, Hyung Joon Joo, Jae Hyoung Park, Chul Min Ahn, Dosun LimAbstract:Black Raspberry (Rubus occidentalis) has been known for its anti-inflammatory and anti-oxidant effects. However, short-term effects of Black Raspberry on lipid profiles and vascular endothelial function have not been investigated in patients with metabolic syndrome. Patients with metabolic syndrome (n = 77) were prospectively randomized into a group with Black Raspberry (n = 39, 750 mg/day) and a placebo group (n = 38) during a 12-week follow-up. Lipid profiles, brachial artery flow-mediated dilatation (baFMD), and inflammatory cytokines such as IL-6, TNF-α, C-reactive protein, adiponectin, sICAM-1, and sVCAM-1 were measured at the baseline and at the 12-week follow-up. Decreases from the baseline in the total cholesterol level (-22.8 ± 30.4 mg/dL vs. -1.9 ± 31.8 mg/dL, p < 0.05, respectively) and total cholesterol/HDL ratio (-0.31 ± 0.64 vs. 0.07 ± 0.58, p < 0.05, respectively) were significantly greater in the group with Black Raspberry than in the placebo group. Increases in baFMD at the 12-week follow-up were significantly greater in the group with Black Raspberry than in the placebo group (0.33 ± 0.44 mm vs. 0.10 ± 0.35 mm, p < 0.05, respectively). Decreases from the baseline in IL-6 (-0.4 ± 1.5 pg/mL vs. -0.1 ± 1.0 pg/mL, p < 0.05, respectively) and TNF-α (-2.9 ± 4.7 pg/mL vs. 0.1 ± 3.6 pg/mL, p < 0.05, respectively) were significantly greater in the group with Black Raspberry. The use of Black Raspberry significantly decreased serum total cholesterol level and inflammatory cytokines, thereby improving vascular endothelial function in patients with metabolic syndrome during the 12-week follow-up.
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Effects of Unripe Black Raspberry Water Extract on Lipid Metabolism and Oxidative Stress in Mice
Korean Journal of Food Science and Technology, 2014Co-Authors: Hye Ran Choi, Jong Tae Jeong, Min Jung Lee, Su Jung Lee, Junghyun Lee, Lee Tae BumAbstract:Hye Ran Choi, Jung-Hyun Lee, Su Jung Lee, Min Jung Lee, Jong Tae Jeong, and Tae-Bum Lee*Gochang Black Raspberry Research InstituteAbstract We examined the effects of unripe Black Raspberry water extract (UBR-W) on lipid metabolism and oxidativestress in mice. C57BL/6J mice were divided into 4 groups: those administered a control diet (CTL), high-fat diet (HFD),UBR-W and simvastatin for 12 weeks. In the HFD group, LDL cholesterol were significantly higher than in the CTLgroup. However, the UBR-W treated group showed dose-dependent reduction of plasma LDL levels. Hepatic total lipid,TC, and malondialdehyde were significantly increased in hyperlipidemic mice. However, supplementation with either UBR-W or simvastatin effectively reduced these lipid profiles and lipid peroxidation. UBR-W increased mRNA expression ofthe LDL receptor, sterol regulatory element binding protein 2 (SREBP2), 3-hydroxy-3-methylglutaryl (HMG)-CoAreductase and ATP-binding cassette transporter A1 (ABCA1) compared to that observed in the HFD group. In addition,UBR-W and simvastatin showed significantly reduced oxidized LDL uptake by the scavenger receptor CD36. These resultssuggest that UBR-W is useful for treatment and prevention of hyperlipidemia and lipid peroxidation.Keywords: Unripe Black Raspberry, High-fat diet, Hyperlipidemia, Lipid metabolism, Oxidative stress
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improvement of cholesterol and blood pressure in fruit leaf and stem extracts from Black Raspberry in vitro
The Korean Journal of Medicinal Crop Science, 2014Co-Authors: Min Jung Lee, Hye Ran Choi, Ji-wung Kwon, Jong Tae Jeong, Su Jung Lee, Junghyun Lee, Kyu Seo Chae, Tae Bum LeeAbstract:We compared effects of fruit, leaf, and stem extracts from Black Raspberry on improvement of cholesterol and blood pressure in HepG2 and HUVEC cells, respectively. Cholesterol secretion was inhibited by water extracts of unripe fruit and stem, but not leaf of Black Raspberry in HepG2 cells. Also, water extracts of unripe fruit, leaf, and stem reduced HMG-CoA reductase activity. Furthermore, nitric oxide production and expression of angiotensin-converting enzyme (ACE) protein were regulated by extracts of fruit, leaf, and stem of Black Raspberry in HUVEC cells. Overall, the rank order according to the improving level of cholesterol and hypertension is as follows: stem > fruit > leaf. In addition, various polyphenol compounds displayed inhibitory effects of HMG-CoA reductase activity and ACE expression. Thus, these data suggested that leaf and stem as wells as fruit of Black Raspberry can be used as useful food resources for reduction of cholesterol and blood pressure.
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Effects of Black Raspberry on lipid profiles and vascular endothelial function in patients with metabolic syndrome.
Phytotherapy research : PTR, 2014Co-Authors: Han Saem Jeong, Ji-wung Kwon, Soon Jun Hong, Tae Bum Lee, Jong Tae Jeong, Hyung Joon Joo, Jae Hyoung Park, Chul Min Ahn, Dosun LimAbstract:Black Raspberry (Rubus occidentalis) has been known for its anti-inflammatory and anti-oxidant effects. However, short-term effects of Black Raspberry on lipid profiles and vascular endothelial function have not been investigated in patients with metabolic syndrome. Patients with metabolic syndrome (n = 77) were prospectively randomized into a group with Black Raspberry (n = 39, 750 mg/day) and a placebo group (n = 38) during a 12-week follow-up. Lipid profiles, brachial artery flow-mediated dilatation (baFMD), and inflammatory cytokines such as IL-6, TNF-α, C-reactive protein, adiponectin, sICAM-1, and sVCAM-1 were measured at the baseline and at the 12-week follow-up. Decreases from the baseline in the total cholesterol level (-22.8 ± 30.4 mg/dL vs. -1.9 ± 31.8 mg/dL, p
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effects of extracts of unripe Black Raspberry and red ginseng on cholesterol synthesis
Korean Journal of Food Science and Technology, 2013Co-Authors: Su Jung Lee, Hye Ran Choi, Jong Tae Jeong, Min Jung Lee, Kyungmin Choi, Jeongdan Cha, Seungmi Hwang, Hoo Kil Jung, Jonghyuk Park, Tae Bum LeeAbstract:We investigated the effects of water extracts of unripe Black Raspberry (UBR) and red ginseng (RG) on cholesterol synthesis, 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase activity, and expression of low-density lipoprotein (LDL) or high-density lipoprotein (HDL)-related genes in HepG2 and Caco-2 (human hepatoma and intestinal cell lines, respectively). Our results showed that cholesterol synthesis and HMG-CoA reductase activity in HepG2 cells were inhibited by UBR and RG. Further, co-treatment with UBR and RG had a greater effect than did treatment with either UBR or RG. In Caco-2 cells, treatment with UBR and RG increased the expression of LDL-regulated genes, such as LDL receptor and SREBP-2, and also upregulated the level of HDL-associated ABCA1. Moreover, co-treatment with UBR and RG appeared to be more effective than treatment with either UBR or RG. Taken together, our results indicate that UBR and RG regulate the level of HDL-associated ABCA1 via signaling pathway, thereby preventing cholesterol synthesis.
Han Saem Jeong - One of the best experts on this subject based on the ideXlab platform.
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Black Raspberry extract increased circulating endothelial progenitor cells and improved arterial stiffness in patients with metabolic syndrome a randomized controlled trial
Journal of Medicinal Food, 2016Co-Authors: Han Saem Jeong, Soon Jun Hong, Tae Bum Lee, Sohyeon Kim, Seung Cheol Choi, Ji Hyun Choi, Jong Ho Kim, Chi Yeon Park, Jae Young Cho, Ji-wung KwonAbstract:Administration of Black Raspberry (Rubus occidentalis) is known to improve vascular endothelial function in patients at a high risk for cardiovascular (CV) disease. We investigated short-term effects of Black Raspberry on circulating endothelial progenitor cells (EPCs) and arterial stiffness in patients with metabolic syndrome. Patients with metabolic syndrome (n = 51) were prospectively randomized into the Black Raspberry group (n = 26, 750 mg/day) and placebo group (n = 25) during the 12-week follow-up. Central blood pressure, augmentation index, and EPCs, such as CD34/KDR(+), CD34/CD117(+), and CD34/CD133(+), were measured at baseline and at 12-week follow-up. Radial augmentation indexes were significantly decreased in the Black Raspberry group compared to the placebo group (-5% ± 10% vs. 3% ± 14%, P < .05). CD34/CD133(+) cells at 12-week follow-up were significantly higher in the Black Raspberry group compared to the placebo group (19 ± 109/μL vs. -28 ± 57/μL, P < .05). Decreases from the baseline in interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were significantly greater in the Black Raspberry group compared to the placebo group (-0.5 ± 1.4 pg/mL vs. -0.1 ± 1.1 pg/mL, P < .05 and -5.4 ± 4.5 pg/mL vs. -0.8 ± 4.0 pg/mL, P < .05, respectively). Increases from the baseline in adiponectin levels (2.9 ± 2.1 μg/mL vs. -0.2 ± 2.5 μg/mL, P < .05) were significant in the Black Raspberry group. The use of Black Raspberry significantly lowered the augmentation index and increased circulating EPCs, thereby improving CV risks in patients with metabolic syndrome during the 12-week follow-up.
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Black Raspberry Extract Increased Circulating Endothelial Progenitor Cells and Improved Arterial Stiffness in Patients with Metabolic Syndrome: A Randomized Controlled Trial
Journal of medicinal food, 2016Co-Authors: Han Saem Jeong, Soon Jun Hong, Tae Bum Lee, Sohyeon Kim, Seung Cheol Choi, Ji Hyun Choi, Jong Ho Kim, Chi Yeon Park, Jae Young Cho, Ji-wung KwonAbstract:Administration of Black Raspberry (Rubus occidentalis) is known to improve vascular endothelial function in patients at a high risk for cardiovascular (CV) disease. We investigated short-term effects of Black Raspberry on circulating endothelial progenitor cells (EPCs) and arterial stiffness in patients with metabolic syndrome. Patients with metabolic syndrome (n = 51) were prospectively randomized into the Black Raspberry group (n = 26, 750 mg/day) and placebo group (n = 25) during the 12-week follow-up. Central blood pressure, augmentation index, and EPCs, such as CD34/KDR(+), CD34/CD117(+), and CD34/CD133(+), were measured at baseline and at 12-week follow-up. Radial augmentation indexes were significantly decreased in the Black Raspberry group compared to the placebo group (-5% ± 10% vs. 3% ± 14%, P
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effects of Black Raspberry on lipid profiles and vascular endothelial function in patients with metabolic syndrome
Phytotherapy Research, 2014Co-Authors: Han Saem Jeong, Ji-wung Kwon, Soon Jun Hong, Tae Bum Lee, Jong Tae Jeong, Hyung Joon Joo, Jae Hyoung Park, Chul Min Ahn, Dosun LimAbstract:Black Raspberry (Rubus occidentalis) has been known for its anti-inflammatory and anti-oxidant effects. However, short-term effects of Black Raspberry on lipid profiles and vascular endothelial function have not been investigated in patients with metabolic syndrome. Patients with metabolic syndrome (n = 77) were prospectively randomized into a group with Black Raspberry (n = 39, 750 mg/day) and a placebo group (n = 38) during a 12-week follow-up. Lipid profiles, brachial artery flow-mediated dilatation (baFMD), and inflammatory cytokines such as IL-6, TNF-α, C-reactive protein, adiponectin, sICAM-1, and sVCAM-1 were measured at the baseline and at the 12-week follow-up. Decreases from the baseline in the total cholesterol level (-22.8 ± 30.4 mg/dL vs. -1.9 ± 31.8 mg/dL, p < 0.05, respectively) and total cholesterol/HDL ratio (-0.31 ± 0.64 vs. 0.07 ± 0.58, p < 0.05, respectively) were significantly greater in the group with Black Raspberry than in the placebo group. Increases in baFMD at the 12-week follow-up were significantly greater in the group with Black Raspberry than in the placebo group (0.33 ± 0.44 mm vs. 0.10 ± 0.35 mm, p < 0.05, respectively). Decreases from the baseline in IL-6 (-0.4 ± 1.5 pg/mL vs. -0.1 ± 1.0 pg/mL, p < 0.05, respectively) and TNF-α (-2.9 ± 4.7 pg/mL vs. 0.1 ± 3.6 pg/mL, p < 0.05, respectively) were significantly greater in the group with Black Raspberry. The use of Black Raspberry significantly decreased serum total cholesterol level and inflammatory cytokines, thereby improving vascular endothelial function in patients with metabolic syndrome during the 12-week follow-up.
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Effects of Black Raspberry on lipid profiles and vascular endothelial function in patients with metabolic syndrome.
Phytotherapy research : PTR, 2014Co-Authors: Han Saem Jeong, Ji-wung Kwon, Soon Jun Hong, Tae Bum Lee, Jong Tae Jeong, Hyung Joon Joo, Jae Hyoung Park, Chul Min Ahn, Dosun LimAbstract:Black Raspberry (Rubus occidentalis) has been known for its anti-inflammatory and anti-oxidant effects. However, short-term effects of Black Raspberry on lipid profiles and vascular endothelial function have not been investigated in patients with metabolic syndrome. Patients with metabolic syndrome (n = 77) were prospectively randomized into a group with Black Raspberry (n = 39, 750 mg/day) and a placebo group (n = 38) during a 12-week follow-up. Lipid profiles, brachial artery flow-mediated dilatation (baFMD), and inflammatory cytokines such as IL-6, TNF-α, C-reactive protein, adiponectin, sICAM-1, and sVCAM-1 were measured at the baseline and at the 12-week follow-up. Decreases from the baseline in the total cholesterol level (-22.8 ± 30.4 mg/dL vs. -1.9 ± 31.8 mg/dL, p