The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
Hisayuki Ueno - One of the best experts on this subject based on the ideXlab platform.
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Detection of Antigen‐Specific T Cells in Experimental Immune‐Mediated Blepharoconjunctivitis in DO11.10 T Cell Receptor Transgenic Mice
Microbiology and Immunology, 2020Co-Authors: Akemi Ozaki, Atsuki Fukushima, Waka Ishida, Kazuyo Fukata, Hisayuki UenoAbstract:Antigen (Ag)-specific T cells are thought to play a key role in pathogenesis of chronic allergic con- junctivitis (AC) such as atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC). In order to investigate the trafficking of Ag-specific T cells in experimental immune-mediated Blepharoconjunctivitis (EC), we established a novel AC model in DO11.10 T cell receptor (TcR) transgenic (Tg) mice. DO11.10 TcR-Tg mice were challenged with eye drops of whole OVA protein, OVA peptide 1-15, 321-335, or 323-339. Their eyes were histologically examined. Conventional proliferation assay was performed against each Ag. Phenotypes of infiltrating cells and kinetics of Ag-specific T cells were investigated by immunohistochemistry. Adoptive transfer of CD4 � Ag-specific T cells from DO11.10 TcR-Tg to WT
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Fyn regulates eosinophil infiltration into the conjunctiva by downregulating the Th2 response
Graefes Archive for Clinical and Experimental Ophthalmology, 2005Co-Authors: Atsuki Fukushima, Tomoko Yamaguchi, Akemi Ozaki, Taketoshi Taniguchi, Keiko Udaka, Hisayuki UenoAbstract:Purpose Under certain circumstances, fyn may serve to negatively regulate the differentiation of naive helper T (Th) cells into Th2 cells. This study aimed to investigate whether fyn negatively regulates the development of experimental immune-mediated Blepharoconjunctivitis (EC), in which Th2 cells play an important role in C57BL/6 mice.
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dissection of antigen specific humoral and cellular immune responses for the development of experimental immune mediated Blepharoconjunctivitis in c57bl 6 mice
Current Eye Research, 2005Co-Authors: Atsuki Fukushima, Akemi Ozaki, Waka Ishida, Kazuyo Fukata, Zhang Jian, Hisayuki UenoAbstract:Purpose: Allergic conjunctivitis is characterized by allergen-specific IgE in the serum and infiltration of eosinophils into the conjunctiva. However, it remains unclear whether early-phase reaction (EPR) mediated by Ag-specific IgE links to late-phase reaction (LPR) in the conjunctiva. We aimed to investigate whether LPR is mediated by either cellular or humoral immune responses. Methods: Experimental immune-mediated Blepharoconjunctivitis (EC) was induced in C57BL/6 mice by either active immunization or passive immunization by transfer of ragweed (RW)-primed lymphocytes and RW-specific IgE, followed by RW challenge onto the conjunctiva. Transferring RW-primed lymphocytes were prepared from RW-primed splenocytes which were stimulated in vitro with RW for 3 days. Fifteen minutes after RW challenge, clinical findings were evaluated and 24 hr after challenge, the conjunctivas and sera were harvested for histologic analysis and measurement of IgE, respectively. Results: EPR was most prominent when EC was ind...
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artificial tears inhibit the development of experimental immune mediated Blepharoconjunctivitis
Japanese Journal of Ophthalmology, 2004Co-Authors: Atsuki Fukushima, Olga Zinchuk, Hisayuki UenoAbstract:Purpose To investigate whether treatment with artificial tears inhibits the development of experimental immune-mediated Blepharoconjunctivitis (EC).
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Artificial Tears Inhibit the Development of Experimental Immune-mediated Blepharoconjunctivitis
Japanese Journal of Ophthalmology, 2004Co-Authors: Atsuki Fukushima, Olga Zinchuk, Hisayuki UenoAbstract:Purpose To investigate whether treatment with artificial tears inhibits the development of experimental immune-mediated Blepharoconjunctivitis (EC). Methods Brown Norway rats were immunized with ovalbumin (OVA) or ragweed (RW) emulsified in complete Freund’s adjuvant. Fourteen days after immunization, the rats were challenged with the same antigen (Ag) in eye drops. Treated rats were administered artificial tears by eye drops immediately after, 15 min after, or 30 min after the Ag challenge. Treatment doses of 2, 4, or 8 drops per eye were evaluated. Twenty-four hours after the Ag challenge, the rats were killed and their eyes were harvested for histological studies. Results Treatment with artificial tears immediately after and 15 min after challenge with partially insoluble RW Ag suppressed infiltration of inflammatory cells into the conjunctiva. Inhibition was not observed at any time following challenge with OVA Ag, which is a soluble protein. The treatment dose of artificial tears administered did not affect the extent of inhibition of EC following challenge with either Ag. Conclusions Treatment with artificial tears by eye drops inhibited the development of EC induced by the partially insoluble RW Ag when administered within 15 min of the Ag challenge. Jpn J Ophthalmol 2004;48:530–534 © Japanese Ophthalmological Society 2004
Atsuki Fukushima - One of the best experts on this subject based on the ideXlab platform.
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Detection of Antigen‐Specific T Cells in Experimental Immune‐Mediated Blepharoconjunctivitis in DO11.10 T Cell Receptor Transgenic Mice
Microbiology and Immunology, 2020Co-Authors: Akemi Ozaki, Atsuki Fukushima, Waka Ishida, Kazuyo Fukata, Hisayuki UenoAbstract:Antigen (Ag)-specific T cells are thought to play a key role in pathogenesis of chronic allergic con- junctivitis (AC) such as atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC). In order to investigate the trafficking of Ag-specific T cells in experimental immune-mediated Blepharoconjunctivitis (EC), we established a novel AC model in DO11.10 T cell receptor (TcR) transgenic (Tg) mice. DO11.10 TcR-Tg mice were challenged with eye drops of whole OVA protein, OVA peptide 1-15, 321-335, or 323-339. Their eyes were histologically examined. Conventional proliferation assay was performed against each Ag. Phenotypes of infiltrating cells and kinetics of Ag-specific T cells were investigated by immunohistochemistry. Adoptive transfer of CD4 � Ag-specific T cells from DO11.10 TcR-Tg to WT
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Fyn regulates eosinophil infiltration into the conjunctiva by downregulating the Th2 response
Graefes Archive for Clinical and Experimental Ophthalmology, 2005Co-Authors: Atsuki Fukushima, Tomoko Yamaguchi, Akemi Ozaki, Taketoshi Taniguchi, Keiko Udaka, Hisayuki UenoAbstract:Purpose Under certain circumstances, fyn may serve to negatively regulate the differentiation of naive helper T (Th) cells into Th2 cells. This study aimed to investigate whether fyn negatively regulates the development of experimental immune-mediated Blepharoconjunctivitis (EC), in which Th2 cells play an important role in C57BL/6 mice.
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dissection of antigen specific humoral and cellular immune responses for the development of experimental immune mediated Blepharoconjunctivitis in c57bl 6 mice
Current Eye Research, 2005Co-Authors: Atsuki Fukushima, Akemi Ozaki, Waka Ishida, Kazuyo Fukata, Zhang Jian, Hisayuki UenoAbstract:Purpose: Allergic conjunctivitis is characterized by allergen-specific IgE in the serum and infiltration of eosinophils into the conjunctiva. However, it remains unclear whether early-phase reaction (EPR) mediated by Ag-specific IgE links to late-phase reaction (LPR) in the conjunctiva. We aimed to investigate whether LPR is mediated by either cellular or humoral immune responses. Methods: Experimental immune-mediated Blepharoconjunctivitis (EC) was induced in C57BL/6 mice by either active immunization or passive immunization by transfer of ragweed (RW)-primed lymphocytes and RW-specific IgE, followed by RW challenge onto the conjunctiva. Transferring RW-primed lymphocytes were prepared from RW-primed splenocytes which were stimulated in vitro with RW for 3 days. Fifteen minutes after RW challenge, clinical findings were evaluated and 24 hr after challenge, the conjunctivas and sera were harvested for histologic analysis and measurement of IgE, respectively. Results: EPR was most prominent when EC was ind...
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artificial tears inhibit the development of experimental immune mediated Blepharoconjunctivitis
Japanese Journal of Ophthalmology, 2004Co-Authors: Atsuki Fukushima, Olga Zinchuk, Hisayuki UenoAbstract:Purpose To investigate whether treatment with artificial tears inhibits the development of experimental immune-mediated Blepharoconjunctivitis (EC).
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Artificial Tears Inhibit the Development of Experimental Immune-mediated Blepharoconjunctivitis
Japanese Journal of Ophthalmology, 2004Co-Authors: Atsuki Fukushima, Olga Zinchuk, Hisayuki UenoAbstract:Purpose To investigate whether treatment with artificial tears inhibits the development of experimental immune-mediated Blepharoconjunctivitis (EC). Methods Brown Norway rats were immunized with ovalbumin (OVA) or ragweed (RW) emulsified in complete Freund’s adjuvant. Fourteen days after immunization, the rats were challenged with the same antigen (Ag) in eye drops. Treated rats were administered artificial tears by eye drops immediately after, 15 min after, or 30 min after the Ag challenge. Treatment doses of 2, 4, or 8 drops per eye were evaluated. Twenty-four hours after the Ag challenge, the rats were killed and their eyes were harvested for histological studies. Results Treatment with artificial tears immediately after and 15 min after challenge with partially insoluble RW Ag suppressed infiltration of inflammatory cells into the conjunctiva. Inhibition was not observed at any time following challenge with OVA Ag, which is a soluble protein. The treatment dose of artificial tears administered did not affect the extent of inhibition of EC following challenge with either Ag. Conclusions Treatment with artificial tears by eye drops inhibited the development of EC induced by the partially insoluble RW Ag when administered within 15 min of the Ag challenge. Jpn J Ophthalmol 2004;48:530–534 © Japanese Ophthalmological Society 2004
Joseph A. Capriotti - One of the best experts on this subject based on the ideXlab platform.
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Rosacea Blepharoconjunctivitis Treated with a Novel Preparation of Dilute Povidone Iodine and Dimethylsulfoxide: a Case Report and Review of the Literature
Ophthalmology and Therapy, 2015Co-Authors: Jesse S. Pelletier, Kevin P. Stewart, Kara Capriotti, Joseph A. CapriottiAbstract:Introduction Povidone iodine (PVP-I) 10% aqueous solution is a commonly utilized anti-septic employed for sterilization of the ocular surface prior to interventional procedures. Dimethylsulfoxide (DMSO) is a well-known skin penetration agent scarcely utilized in ophthalmic drug formulations. We describe here a low-dose formulation of 1% PVP-I (w/w) in a gel containing DMSO for use in the setting of recalcitrant rosacea Blepharoconjunctivitis. A review of the ocular uses of dimethylsulfoxide is also presented. Case report A 78-year-old male presented with chronic, long-standing blepharitis involving both the anterior and posterior lid margins. Posterior lid and skin inflammatory changes were consistent with ocular rosacea. Previous oral and topical therapies had been largely ineffective at controlling his condition. Conclusion The topical PVP-I/DMSO system was effective in abating the signs and symptoms of rosacea Blepharoconjunctivitis. Further investigation of this novel agent is warranted.
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rosacea Blepharoconjunctivitis treated with a novel preparation of dilute povidone iodine and dimethylsulfoxide a case report and review of the literature
Ophthalmology and therapy, 2015Co-Authors: Jesse Pelletier, Kara Capriotti, Kevin Stewart, Joseph A. CapriottiAbstract:Introduction Povidone iodine (PVP-I) 10% aqueous solution is a commonly utilized anti-septic employed for sterilization of the ocular surface prior to interventional procedures. Dimethylsulfoxide (DMSO) is a well-known skin penetration agent scarcely utilized in ophthalmic drug formulations. We describe here a low-dose formulation of 1% PVP-I (w/w) in a gel containing DMSO for use in the setting of recalcitrant rosacea Blepharoconjunctivitis. A review of the ocular uses of dimethylsulfoxide is also presented.
B Guillot - One of the best experts on this subject based on the ideXlab platform.
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long lasting allergic contact Blepharoconjunctivitis to phenylephrine eyedrops
Allergy, 2009Co-Authors: N Raisonpeyron, A Du Thanh, P Demoly, B GuillotAbstract:Allergic contact dermatitis due to mydriatic eyedrops is rare despite extensively used by ophthalmologists. Phenylephrine is responsible for most of the cases in the literature. We reported two other cases due to phenylephrine eyedrops with an unusual evolution characterized by chronic debilitating Blepharoconjunctivitis.
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Long‐lasting allergic contact Blepharoconjunctivitis to phenylephrine eyedrops
Allergy, 2009Co-Authors: Nadia Raison-peyron, A Du Thanh, P Demoly, B GuillotAbstract:Allergic contact dermatitis due to mydriatic eyedrops is rare despite extensively used by ophthalmologists. Phenylephrine is responsible for most of the cases in the literature. We reported two other cases due to phenylephrine eyedrops with an unusual evolution characterized by chronic debilitating Blepharoconjunctivitis.
Christopher S. Foster - One of the best experts on this subject based on the ideXlab platform.
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OCULAR SURFACE NEOPLASIA MASQUERADING AS CHRONIC Blepharoconjunctivitis
Cornea, 1999Co-Authors: Esen K. Akpek, Wasee Polcharoen, Roxanne Chan, Christopher S. FosterAbstract:PurposeTo present the clinical characteristics and difficulties in the diagnosis of various ocular surface malignancies mimicking features of chronic Blepharoconjunctivitis and to summarize the current therapeutic approach and prognosis of patients.MethodsSix patients with slowly evolving signs of p
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ocular surface neoplasia masquerading as chronic Blepharoconjunctivitis
Cornea, 1999Co-Authors: Esen K. Akpek, Wasee Polcharoen, Roxanne Chan, Christopher S. FosterAbstract:PURPOSE: To present the clinical characteristics and difficulties in the diagnosis of various ocular surface malignancies mimicking features of chronic Blepharoconjunctivitis and to summarize the current therapeutic approach and prognosis of patients. METHODS: Six patients with slowly evolving signs of persistent inflammation underwent a conjunctival biopsy after a prolonged course of medical treatment. The medical records of the patients were reviewed. RESULTS: Histopathologic examination of the biopsy specimens revealed intraepithelial squamous neoplasia (one patient), invasive squamous cell carcinoma (one patient), sebaceous carcinoma (two patients), and conjunctival lymphoma (two patients). CONCLUSION: Although uncommon, ocular surface malignancies may involve the conjunctiva diffusely and present as chronic conjunctivitis. A high index of suspicion and an early histopathologic examination are essential to not delay diagnosis.