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Blepharoconjunctivitis

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Hisayuki Ueno – 1st expert on this subject based on the ideXlab platform

  • Detection of Antigen‐Specific T Cells in Experimental Immune‐Mediated Blepharoconjunctivitis in DO11.10 T Cell Receptor Transgenic Mice
    Microbiology and Immunology, 2020
    Co-Authors: Akemi Ozaki, Atsuki Fukushima, Waka Ishida, Kazuyo Fukata, Hisayuki Ueno

    Abstract:

    Antigen (Ag)-specific T cells are thought to play a key role in pathogenesis of chronic allergic con- junctivitis (AC) such as atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC). In order to investigate the trafficking of Ag-specific T cells in experimental immune-mediated Blepharoconjunctivitis (EC), we established a novel AC model in DO11.10 T cell receptor (TcR) transgenic (Tg) mice. DO11.10 TcR-Tg mice were challenged with eye drops of whole OVA protein, OVA peptide 1-15, 321-335, or 323-339. Their eyes were histologically examined. Conventional proliferation assay was performed against each Ag. Phenotypes of infiltrating cells and kinetics of Ag-specific T cells were investigated by immunohistochemistry. Adoptive transfer of CD4 � Ag-specific T cells from DO11.10 TcR-Tg to WT

  • Fyn regulates eosinophil infiltration into the conjunctiva by downregulating the Th2 response
    Graefes Archive for Clinical and Experimental Ophthalmology, 2005
    Co-Authors: Atsuki Fukushima, Tomoko Yamaguchi, Akemi Ozaki, Taketoshi Taniguchi, Keiko Udaka, Hisayuki Ueno

    Abstract:

    Purpose
    Under certain circumstances, fyn may serve to negatively regulate the differentiation of naive helper T (Th) cells into Th2 cells. This study aimed to investigate whether fyn negatively regulates the development of experimental immune-mediated Blepharoconjunctivitis (EC), in which Th2 cells play an important role in C57BL/6 mice.

  • dissection of antigen specific humoral and cellular immune responses for the development of experimental immune mediated Blepharoconjunctivitis in c57bl 6 mice
    Current Eye Research, 2005
    Co-Authors: Atsuki Fukushima, Akemi Ozaki, Waka Ishida, Kazuyo Fukata, Zhang Jian, Hisayuki Ueno

    Abstract:

    Purpose: Allergic conjunctivitis is characterized by allergen-specific IgE in the serum and infiltration of eosinophils into the conjunctiva. However, it remains unclear whether early-phase reaction (EPR) mediated by Ag-specific IgE links to late-phase reaction (LPR) in the conjunctiva. We aimed to investigate whether LPR is mediated by either cellular or humoral immune responses. Methods: Experimental immune-mediated Blepharoconjunctivitis (EC) was induced in C57BL/6 mice by either active immunization or passive immunization by transfer of ragweed (RW)-primed lymphocytes and RW-specific IgE, followed by RW challenge onto the conjunctiva. Transferring RW-primed lymphocytes were prepared from RW-primed splenocytes which were stimulated in vitro with RW for 3 days. Fifteen minutes after RW challenge, clinical findings were evaluated and 24 hr after challenge, the conjunctivas and sera were harvested for histologic analysis and measurement of IgE, respectively. Results: EPR was most prominent when EC was ind…

Atsuki Fukushima – 2nd expert on this subject based on the ideXlab platform

  • Detection of Antigen‐Specific T Cells in Experimental Immune‐Mediated Blepharoconjunctivitis in DO11.10 T Cell Receptor Transgenic Mice
    Microbiology and Immunology, 2020
    Co-Authors: Akemi Ozaki, Atsuki Fukushima, Waka Ishida, Kazuyo Fukata, Hisayuki Ueno

    Abstract:

    Antigen (Ag)-specific T cells are thought to play a key role in pathogenesis of chronic allergic con- junctivitis (AC) such as atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC). In order to investigate the trafficking of Ag-specific T cells in experimental immune-mediated Blepharoconjunctivitis (EC), we established a novel AC model in DO11.10 T cell receptor (TcR) transgenic (Tg) mice. DO11.10 TcR-Tg mice were challenged with eye drops of whole OVA protein, OVA peptide 1-15, 321-335, or 323-339. Their eyes were histologically examined. Conventional proliferation assay was performed against each Ag. Phenotypes of infiltrating cells and kinetics of Ag-specific T cells were investigated by immunohistochemistry. Adoptive transfer of CD4 � Ag-specific T cells from DO11.10 TcR-Tg to WT

  • Fyn regulates eosinophil infiltration into the conjunctiva by downregulating the Th2 response
    Graefes Archive for Clinical and Experimental Ophthalmology, 2005
    Co-Authors: Atsuki Fukushima, Tomoko Yamaguchi, Akemi Ozaki, Taketoshi Taniguchi, Keiko Udaka, Hisayuki Ueno

    Abstract:

    Purpose
    Under certain circumstances, fyn may serve to negatively regulate the differentiation of naive helper T (Th) cells into Th2 cells. This study aimed to investigate whether fyn negatively regulates the development of experimental immune-mediated Blepharoconjunctivitis (EC), in which Th2 cells play an important role in C57BL/6 mice.

  • dissection of antigen specific humoral and cellular immune responses for the development of experimental immune mediated Blepharoconjunctivitis in c57bl 6 mice
    Current Eye Research, 2005
    Co-Authors: Atsuki Fukushima, Akemi Ozaki, Waka Ishida, Kazuyo Fukata, Zhang Jian, Hisayuki Ueno

    Abstract:

    Purpose: Allergic conjunctivitis is characterized by allergen-specific IgE in the serum and infiltration of eosinophils into the conjunctiva. However, it remains unclear whether early-phase reaction (EPR) mediated by Ag-specific IgE links to late-phase reaction (LPR) in the conjunctiva. We aimed to investigate whether LPR is mediated by either cellular or humoral immune responses. Methods: Experimental immune-mediated Blepharoconjunctivitis (EC) was induced in C57BL/6 mice by either active immunization or passive immunization by transfer of ragweed (RW)-primed lymphocytes and RW-specific IgE, followed by RW challenge onto the conjunctiva. Transferring RW-primed lymphocytes were prepared from RW-primed splenocytes which were stimulated in vitro with RW for 3 days. Fifteen minutes after RW challenge, clinical findings were evaluated and 24 hr after challenge, the conjunctivas and sera were harvested for histologic analysis and measurement of IgE, respectively. Results: EPR was most prominent when EC was ind…

Joseph A. Capriotti – 3rd expert on this subject based on the ideXlab platform

  • Rosacea Blepharoconjunctivitis Treated with a Novel Preparation of Dilute Povidone Iodine and Dimethylsulfoxide: a Case Report and Review of the Literature
    Ophthalmology and Therapy, 2015
    Co-Authors: Jesse S. Pelletier, Kevin P. Stewart, Kara Capriotti, Joseph A. Capriotti

    Abstract:

    Introduction Povidone iodine (PVP-I) 10% aqueous solution is a commonly utilized anti-septic employed for sterilization of the ocular surface prior to interventional procedures. Dimethylsulfoxide (DMSO) is a well-known skin penetration agent scarcely utilized in ophthalmic drug formulations. We describe here a low-dose formulation of 1% PVP-I (w/w) in a gel containing DMSO for use in the setting of recalcitrant rosacea Blepharoconjunctivitis. A review of the ocular uses of dimethylsulfoxide is also presented. Case report A 78-year-old male presented with chronic, long-standing blepharitis involving both the anterior and posterior lid margins. Posterior lid and skin inflammatory changes were consistent with ocular rosacea. Previous oral and topical therapies had been largely ineffective at controlling his condition. Conclusion The topical PVP-I/DMSO system was effective in abating the signs and symptoms of rosacea Blepharoconjunctivitis. Further investigation of this novel agent is warranted.

  • rosacea Blepharoconjunctivitis treated with a novel preparation of dilute povidone iodine and dimethylsulfoxide a case report and review of the literature
    Ophthalmology and therapy, 2015
    Co-Authors: Jesse Pelletier, Kara Capriotti, Kevin Stewart, Joseph A. Capriotti

    Abstract:

    Introduction
    Povidone iodine (PVP-I) 10% aqueous solution is a commonly utilized anti-septic employed for sterilization of the ocular surface prior to interventional procedures. Dimethylsulfoxide (DMSO) is a well-known skin penetration agent scarcely utilized in ophthalmic drug formulations. We describe here a low-dose formulation of 1% PVP-I (w/w) in a gel containing DMSO for use in the setting of recalcitrant rosacea Blepharoconjunctivitis. A review of the ocular uses of dimethylsulfoxide is also presented.