Bradyrhizobium japonicum - Explore the Science & Experts | ideXlab

Scan Science and Technology

Contact Leading Edge Experts & Companies

Bradyrhizobium japonicum

The Experts below are selected from a list of 7935 Experts worldwide ranked by ideXlab platform

Manabu Itakura – 1st expert on this subject based on the ideXlab platform

  • Linked Expressions of nap and nos Genes in a Bradyrhizobium japonicum Mutant with Increased N2O Reductase Activity
    Applied and Environmental Microbiology, 2013
    Co-Authors: Cristina Sánchez, Manabu Itakura, Hisayuki Mitsui, Kiwamu Minamisawa


    ABSTRACT To understand the mechanisms underlying the increased N2O reductase activity in the Bradyrhizobium japonicum 5M09 mutant from enrichment culture under N2O respiration, we analyzed the expression of genes encoding denitrification reductases and regulators. Our results suggest a common regulation of nap (encoding periplasmic nitrate reductase) and nos (encoding N2O reductase).

  • mitigation of nitrous oxide emissions from soils by Bradyrhizobium japonicum inoculation
    Nature Climate Change, 2013
    Co-Authors: Manabu Itakura, Yoshitaka Uchida, Hiroko Akiyama, Yuko Takada Hoshino, Yumi Shimomura, Sho Morimoto, Kanako Tago, Yong Wang, Chihiro Hayakawa, Yusuke Uetake


    Soybean hosts the symbiotic nitrogen-fixing soil bacterium Bradyrhizobium japonicum, that can produce the greenhouse gas nitrous oxide. This study shows that nitrous oxide emissions from soybean ecosystems can be biologically mitigated at a field scale by inoculation with strains of B. japonicum that have increased nitrous oxide reductase activity.

  • the type iii secretion system of Bradyrhizobium japonicum usda122 mediates symbiotic incompatibility with rj2 soybean plants
    Applied and Environmental Microbiology, 2013
    Co-Authors: Takahiro Tsukui, Manabu Itakura, Takakazu Kaneko, Hisayuki Mitsui, Shusei Sato, Shin Okazaki, Kaori Kakizakichiba, Akifumi Yamashita, Kimihiro Terasawa, Kiwamu Minamisawa


    ABSTRACT The rhcJ and ttsI mutants of Bradyrhizobium japonicum USDA122 for the type III protein secretion system (T3SS) failed to secrete typical effector proteins and gained the ability to nodulate Rj2 soybean plants (Hardee), which are symbiotically incompatible with wild-type USDA122. This suggests that effectors secreted via the T3SS trigger incompatibility between these two partners.

Mary Lou Guerinot – 2nd expert on this subject based on the ideXlab platform

  • Succinate dehydrogenase (Sdh) from Bradyrhizobium japonicum is closely related to mitochondrial Sdh.
    Journal of Bacteriology, 1999
    Co-Authors: David J. Westenberg, Mary Lou Guerinot


    The sdhCDAB operon, encoding succinate dehydrogenase, was cloned from the soybean symbiont Bradyrhizobium japonicum. Sdh from B. japonicum is phylogenetically related to Sdh from mitochondria. This is the first example of a mitochondrion-like Sdh functionally expressed in Escherichia coli.

  • Effect ofIronAvailability on Expression ofthe Bradyrhizobium japonicum hemAGene
    , 1994
    Co-Authors: Mary Lou Guerinot


    bacterial cytochromes andoxidases whichoperate inthemicroaerophilic environment foundin nodules (20, 28)andFixL, abacterial hemoprotein (15) which isbelieved tofunction asanoxygen sensor toregulate expression ofthenitrogen fixation (nif) genes (10). Theuniversal first step inthesynthesis ofall tetrapyrroles, including heme, isthe formation ofA-aminolevulinic acid(ALA). Thesoybean symbiont Bradyrhizobium japonicum, like other members ofthe ao-subgroup ofpurple bacteria (2), produces ALA byaonestep condensation ofglycine andsuccinyl-coenzyme A toform

  • Effect of iron availability on expression of the Bradyrhizobium japonicum hemA gene.
    Journal of Bacteriology, 1994
    Co-Authors: K M Page, Erin L. Connolly, Mary Lou Guerinot


    Bradyrhizobium japonicum produces delta-aminolevulinic acid, the universal precursor of tetrapyrroles, in a reaction catalyzed by the product of the hemA gene. Expression of the B. japonicum hemA gene is affected by iron availability. Activity of a hemA-lacZ fusion is increased approximately threefold by iron, and RNA analysis indicates that iron regulation is at the level of mRNA accumulation. To our knowledge, this is the first example of an iron-regulated heme biosynthetic gene in prokaryotes.

Michael Göttfert – 3rd expert on this subject based on the ideXlab platform

  • The genistein stimulon of Bradyrhizobium japonicum
    Molecular Genetics and Genomics, 2008
    Co-Authors: Kathrin Lang, Andrea Lindemann, Felix Hauser, Michael Göttfert


    An initializing step in the rhizobia–legume symbiosis is the secretion of flavonoids by plants that leads to the expression of nodulation genes in rhizobia. Here we report the genome-wide transcriptional response of Bradyrhizobium japonicum to genistein, an isoflavone secreted by soybean. About 100 genes were induced in the wild type. This included all nod box-associated genes, the flagellar cluster and several genes that are likely to be involved in transport processes. To elucidate the role of known regulators, we analysed mutant strains. This revealed that the two-component response regulator NodW is essential for induction of almost all genistein-inducible genes, with the exception of 8 genes. The phenotype of the nodW mutant could be partially suppressed by overexpression of NwsB, which is also a two-component response regulator. These data indicate that genistein has a much broader function than mere induction of nod genes.

  • A single rRNA gene region in Bradyrhizobium japonicum.
    Journal of Bacteriology, 1995
    Co-Authors: C Kündig, Hauke Hennecke, Christoph F. Beck, Michael Göttfert


    Bradyrhizobium japonicum contains only a single rRNA (rrn) gene region, despite its comparatively large genome size of 8,700 kb. The nucleotide sequence revealed an organization of rRNA and tRNA genes that is frequently found in bacteria: 5′-rrs (16S rRNA)-ileT (tRNA(Ile))-alaT (tRNA(Ala))-rrl (23S rRNA)-rrf (5S rRNA)-3′. The 5′ end of the primary transcript, one of the 16S rRNA processing sites, and the 5′ end of the mature 16S rRNA were determined by primer extension. DNA hybridization experiments showed that the slowly growing Bradyrhizobium strains generally have only a single copy of the 16S rRNA gene, whereas the faster-growing Rhizobium species contain three rrs copies.