The Experts below are selected from a list of 5637 Experts worldwide ranked by ideXlab platform
Maricelis Acevedo - One of the best experts on this subject based on the ideXlab platform.
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inheritance and Bulked Segregant Analysis of leaf rust and stem rust resistance in durum wheat genotypes
Phytopathology, 2017Co-Authors: Meriem Aoun, J A Kolmer, Matthew N Rouse, Shiaoman Chao, Worku Denbel Bulbula, Elias M Elias, Maricelis AcevedoAbstract:Leaf rust, caused by Puccinia triticina, and stem rust, caused by P. graminis f. sp. tritici, are important diseases of durum wheat. This study determined the inheritance and genomic locations of leaf rust resistance (Lr) genes to P. triticina race BBBQJ and stem rust resistance (Sr) genes to P. graminis f. sp. tritici race TTKSK in durum accessions. Eight leaf-rust-resistant genotypes were used to develop biparental populations. Accessions PI 192051 and PI 534304 were also resistant to P. graminis f. sp. tritici race TTKSK. The resulting progenies were phenotyped for leaf rust and stem rust response at seedling stage. The Lr and Sr genes were mapped in five populations using single-nucleotide polymorphisms and Bulked Segregant Analysis. Five leaf-rust-resistant genotypes carried single dominant Lr genes whereas, in the remaining accessions, there was deviation from the expected segregation ratio of a single dominant Lr gene. Seven genotypes carried Lr genes different from those previously characterized i...
Lining Zhao - One of the best experts on this subject based on the ideXlab platform.
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identification and validation of single nucleotide polymorphism markers linked to first flower node in kenaf by using combined specific locus amplified fragment sequencing and Bulked Segregant Analysis
Industrial Crops and Products, 2019Co-Authors: Hui Li, Defang Li, Lining ZhaoAbstract:Abstract It is important to develop DNA markers closely linked to the first flower node for molecular marker-assisted selection in kenaf breeding. Kenaf (Hibiscus cannabinus L.) is an annual, multipurpose industrial crop with a worldwide distribution. The higher the node of the first flower, the greater is the fiber yield. In this study, a population of 130 F2 individuals was constructed through the cross of K215(♀)×K89(♂). Twenty-five individuals with higher first flower node and twenty-five individuals with lower first flower node were chosen and their DNA were pooled to construct two Bulked DNA pools according to the phenotype. Specific-locus amplified fragment sequencing (SLAF-seq) combined with Bulked Segregant Analysis (BSA) was used to identify candidate DNA markers closely linked to the first flower node. Sixteen single-nucleotide polymorphism (SNP) loci were obtained from 11 related SLAF markers associated with the first flower node. SNP locus validation was performed using Sanger sequencing method. The SNP locus S961-2 in Sanger sequencing by using the primer M41961 was consistent with the SNP locus in the related SLAF-seq. The accuracy rate of the genotypes consistent with the first flower node was 91.2% (31/34). To our knowledge, S961-2 is the first SNP locus to be identified that is closely linked to the first flower node. This SNP locus may be useful for marker-assisted selection in breeding of high fiber yielding varieties of kenaf.
Lei Wang - One of the best experts on this subject based on the ideXlab platform.
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quantitative trait loci detection of edwardsiella tarda resistance in japanese flounder paralichthys olivaceus using Bulked Segregant Analysis
Chinese Journal of Oceanology and Limnology, 2016Co-Authors: Xiaoxia Wang, Wenteng Xu, Lei Wang, Songlin ChenAbstract:In recent years, Edwardsiella tarda has become one of the most deadly pathogens of Japanese flounder (Paralichthys olivaceus), causing serious annual losses in commercial production. In contrast to the rapid advances in the aquaculture of P. olivaceus, the study of E. tarda resistance-related markers has lagged behind, hindering the development of a disease-resistant strain. Thus, a marker-trait association Analysis was initiated, combining Bulked Segregant Analysis (BSA) and quantitative trait loci (QTL) mapping. Based on 180 microsatellite loci across all chromosomes, 106 individuals from the F1333 (♀: F0768 ×♂: F0915) (Nomenclature rule: F+year+family number) were used to detect simple sequence repeats (SSRs) and QTLs associated with E. tarda resistance. After a genomic scan, three markers (Scaffold 404-21589, Scaffold 404-21594 and Scaffold 270-13812) from the same linkage group (LG)-1 exhibited a significant difference between DNA, pooled/Bulked from the resistant and susceptible groups (P <0.001). Therefore, 106 individuals were genotyped using all the SSR markers in LG1 by single marker Analysis. Two different analytical models were then employed to detect SSR markers with different levels of significance in LG1, where 17 and 18 SSR markers were identified, respectively. Each model found three resistance-related QTLs by composite interval mapping (CIM). These six QTLs, designated qE1–6, explained 16.0%–89.5% of the phenotypic variance. Two of the QTLs, qE-2 and qE-4, were located at the 66.7 cM region, which was considered a major candidate region for E. tarda resistance. This study will provide valuable data for further investigations of E. tarda resistance genes and facilitate the selective breeding of disease-resistant Japanese flounder in the future.
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a genome scan for quantitative trait loci associated with vibrio anguillarum infection resistance in japanese flounder paralichthys olivaceus by Bulked Segregant Analysis
Marine Biotechnology, 2014Co-Authors: Lei Wang, Yingping Zhang, Han Deng, Ying Xu, Yongsheng Tian, Xiaolin Liao, Wenlong Li, Songlin ChenAbstract:A recent genetic linkage map was employed to detect quantitative trait loci (QTLs) associated with Vibrio anguillarum resistance in Japanese flounder. An F1 family established and challenged with V. anguillarum in 2009 was used for QTL mapping. Of the 221 simple sequence repeat (SSR) markers used to detect polymorphisms in the parents of F1, 170 were confirmed to be polymorphic. The average distance between the markers was 10.6 cM. Equal amounts of genomic DNA from 15 fry that died early and from 15 survivors were pooled separately to constitute susceptible bulk and resistance bulk DNA. Bulked Segregant Analysis and QTL mapping were combined to detect candidate SSR markers and regions associated with the disease. A genome scan identified four polymorphic SSR markers, two of which were significantly different between susceptible and resistance bulk (P = 0.008). These two markers were located in linkage group (LG) 7; therefore, all the SSR markers in LG7 were genotyped in all the challenged fry by single marker Analysis. Using two different models, 11–17 SSR markers were detected with different levels of significance. To confirm the associations of these markers with the disease, composite interval mapping was employed to genotype all the challenged individuals. One and three QTLs, which explained more than 60 % of the phenotypic variance, were detected by the two models. Two of the QTLs were located at 48.6 cM. The common QTL may therefore be a major candidate region for disease resistance against V. anguillarum infection.
F Vedel - One of the best experts on this subject based on the ideXlab platform.
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identification of rapd markers linked to a locus involved in quantitative resistance to tylcv in tomato by Bulked Segregant Analysis
Theoretical and Applied Genetics, 1997Co-Authors: V Chague, J C Mercier, M Guenard, A G L De Courcel, F VedelAbstract:In tomato, Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to a quantitative trait locus (QTL) involved in the resistance to the Tomato Yellow Leaf Curl Virus. F4 lines were distributed into two pools, each consisting of the most resistant and of the most susceptible individuals, respectively. Both pools were screened using 600 random primers. Four RAPD markers were found to be linked to a QTL responsible for up to 27.7% of the resistance. These markers, localized in the same linkage group within a distance of 17.3 cM, were mapped to chromosome 6 on the tomato RFLP map.
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identification and mapping on chromosome 9 of rapd markers linked to sw 5 in tomato by Bulked Segregant Analysis
Theoretical and Applied Genetics, 1996Co-Authors: V Chague, J C Mercier, M Guenard, A G L De Courcel, F VedelAbstract:Bulked Segregant Analysis was used to identify random amplified polymorphic DNA (RAPD) markers linked to the Sw-5 gene for resistance to tomato spotted wilt virus (TSWV) in tomato. Using two pools of phenotyped individuals from one segregating population, we identified four RAPD markers linked to the gene of interest. Two of these appeared tightly linked to Sw-5, whereas another, linked in repulsion phase, enabled the identification of heterozygous and susceptible plants. After linkage Analysis of an F2 population, the RAPD markers were shown to be linked to Sw-5 within a distance of 10.5 cM. One of the RAPD markers close to Sw-5 was used to develop a SCAR (sequence characterized amplified region) marker. Another RAPD marker was stabilized into a pseudo-SCAR marker by enhancing the specificity of its primer sequence without cloning and sequencing. RAPD markers were mapped to chromosome 9 on the RFLP tomato map developed by Tanksley et al. (1992). The Analysis of 13 F3 families and eight BC2 populations segregating for resistance to TSWV confirmed the linkage of the RAPD markers found. These markers are presently being used in marker-assisted plant breeding.
Ruiheng Du - One of the best experts on this subject based on the ideXlab platform.
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combining next generation sequencing with Bulked Segregant Analysis to fine map a stem moisture locus in sorghum sorghum bicolor l moench
PLOS ONE, 2015Co-Authors: Peng Lv, Suying Li, Guisu Ji, Ruiheng DuAbstract:Sorghum is one of the most promising bioenergy crops. Stem juice yield, together with stem sugar concentration, determines sugar yield in sweet sorghum. Bulked Segregant Analysis (BSA) is a gene mapping technique for identifying genomic regions containing genetic loci affecting a trait of interest that when combined with deep sequencing could effectively accelerate the gene mapping process. In this study, a dry stem sorghum landrace was characterized and the stem water controlling locus, qSW6, was fine mapped using QTL Analysis and the combined BSA and deep sequencing technologies. Results showed that: (i) In sorghum variety Jiliang 2, stem water content was around 80% before flowering stage. It dropped to 75% during grain filling with little difference between different internodes. In landrace G21, stem water content keeps dropping after the flag leaf stage. The drop from 71% at flowering time progressed to 60% at grain filling time. Large differences exist between different internodes with the lowest (51%) at the 7th and 8th internodes at dough stage. (ii) A quantitative trait locus (QTL) controlling stem water content mapped on chromosome 6 between SSR markers Ch6-2 and gpsb069 explained about 34.7-56.9% of the phenotypic variation for the 5th to 10th internodes, respectively. (iii) BSA and deep sequencing Analysis narrowed the associated region to 339 kb containing 38 putative genes. The results could help reveal molecular mechanisms underlying juice yield of sorghum and thus to improve total sugar yield.
