The Experts below are selected from a list of 90 Experts worldwide ranked by ideXlab platform
Viktor Chelomin - One of the best experts on this subject based on the ideXlab platform.
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The role of the Cadmium-Binding Protein response of the digestive gland of the Yesso scallop Mizuhopecten yessoensis (Jay, 1857) for marine environmental assessments
Environmental Science and Pollution Research, 2021Co-Authors: Avianna Zhukovskaya, Vladimir Goryachev, Maksim Zakhartsev, Viktor ChelominAbstract:The ability of Pectinidae to accumulate heavy metals and store them in their tissues allows the use of scallops for biomonitoring marine pollution. High molecular weight metallothionein (MT)-like Proteins (MTlps) play a central role in this process. Two major MTlps (72 and 43 kDa) have been identified in the digestive glands of Mizuhopecten yessoensis (Yesso scallop). These Proteins have a very high affinity for the heavy metals Cadmium, cobalt, and caesium. Additionally, these Proteins can be deposited in large quantities in the digestive glands of this mollusc. It has been shown that 72 kDa MTlp is the main stress-response Protein in areas polluted with Cadmium or radioactive metals. Monitoring the amounts of MTlps in the digestive glands of the scallop M. yessoensis in areas with different anthropogenic pollutants has shown that these Proteins are reliable biological markers of heavy-metal pollution in the marine environment.
Hisayoshi Ohta - One of the best experts on this subject based on the ideXlab platform.
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role of metallothionein like Cadmium Binding Protein mtlcdbp in the protective mechanism against Cadmium toxicity in the testis
Industrial Health, 2019Co-Authors: Hisayoshi Ohta, Kenichi Ohba, Tatsushi Toyooka, Ruisheng WangAbstract:The role of metallothionein (MT)-like Cadmium (Cd) Binding Protein (MTLCdBP) in protecting the testes against Cd toxicity was examined. In the acute Cd exposure treatment, Cadmium chloride was intraperitoneally injected at 2 mg Cd/kg to Wistar male rats. In the chronic Cd toxicity treatments, 20 mg Cd/kg/d was orally administered for 5 d a week for 5, 10, and 15 wk. MT (-I,-II) and MTLCdBP were measured using ELISA and Cd-Hem methods, respectively. Testicular tissues were immunostained with antibodies of MT-I,-II, MT-III, and MTLCdBP. Expression of HO1, OGG, iNOS, COX2, and p53 was measured by RT-PCR. Cd concentration in the testis increased dose-dependently in response to Cd exposure. MTLCdBP concentration increased markedly with increasing Cd accumulation. Significant increases in expression of iNOS, HO1, COX2, and OGG1 were observed in the acute exposure treatment. In the chronic oral administration group, expression of MT-I, MT-II, MT-III, iNOS, HO1, and COX2 did not change. Positive immunostaining of MTLCdBP was observed in testicular interstitial tissue. In the testis protected from Cd toxicity, MTLCdBP induction increased significantly with increasing Cd accumulation. Our results suggest that MTLCdBP plays an important role in protecting the testis against Cd toxicity.
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induction of metallothionein like Cadmium Binding Protein in testis and its protective role against Cadmium toxicity
1999Co-Authors: Hisayoshi Ohta, Yukio Seki, Hideyuki Tanaka, Satoshi Asami, Hiroshi YoshikawaAbstract:Despite the numerous studies conducted on the testicular Cadmium (Cd) Binding Protein (Cd-BP) or metallothionein (MT), there is still no consensus on whether the testicular Cd-BP is the same as MT. Moreover, it is unclear whether the testicular Cd-BP is inducible. Therefore, the possible induction of MT-like Cd-BP was investigated in rat testis after oral Cd administration and Cd injection.
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induction of metallothionein like Cadmium Binding Protein in the testis by oral Cadmium administration in rats
Industrial Health, 1997Co-Authors: Hisayoshi Ohta, Yukio Seki, Minoru Nakakita, Hideyuki Tanaka, Hiroshi YoshikawaAbstract:The possible induction of a metallothionein (MT)-like Cadmium (Cd) Binding Protein (MT-like Cd-BP) was investigated in rat testis after oral Cd administration. Male Wistar rats were given Cd by oral administration (20mgCd/kg, for 10 weeks), while the experimental controls were given Cd by intraperitoneal (ip) injection (2mgCd/kg). Cd concentration increased in the testes after both administrations. However, much more Cd (about 4 times) accumulated in the testes of rats receiving oral Cd administration than in rats receiving Cd ip injection (experimental control). Meanwhile, MT-like Cd-BP decreased dramatically in the testes after Cd ip injection compared to that in the testes of untreated control rats. However, this testicular MT-like Cd-BP after oral Cd administration increased significantly up to about 1.4 times of the amount found in the testes of untreated control rats. Inhibition of glutathione S-transferase (GST) activity and decreased glutathione (GSH) in the testes was not observed in rats after oral Cd administration. However, enzyme activity and GSH concentration were inhibited and decreased significantly in the testis by Cd toxicity after Cd ip injection. These results indicate that testicular MT-like Cd-BP, assumed to be MT and to be hardly inducible by Cd, is an inducible Protein corresponding to increased Cd accumulation in the testis without damage by Cd toxicity after oral Cd administration.
Dieter Ammermann - One of the best experts on this subject based on the ideXlab platform.
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effects of Cadmium in stylonychia lemnae stylonychia notophora and oxytricha granulifera isolation of a Cadmium Binding Protein
Journal of Eukaryotic Microbiology, 1992Co-Authors: Ester Piccinni, Paola Irato, L Cavallini, Dieter AmmermannAbstract:The effects of Cadmium on three ciliates are reported here. Cultures of Stylonychia lemnae, Stylonychia notophora and Oxytricha granulifera were treated with different doses of Cd according to tolerance. The two species of Stylonychia are very sensitive to the metal, white O. granulifera tolerates higher doses. Adding 50 μM of Cd to the medium did not damage cells. The accumulated metal is almost totally present in the particulate fraction after day 3. Two Cd-Zn linking fractions were separated from the soluble fraction of culture treated on day 1. The first Protein linking 17 μg Cd/mg showed an ultraviolet absorption spectrum similar to that of Cd-thioneins. Preliminary amino acid analyses indicated that it contained 13% cysteine. The second Protein, linking 60 μg Cd/mg, was a glycoProtein. Its ultraviolet absorption spectrum and amino acid analysis showed that this Binding Protein was far from being a metallothionein: its cysteine content was very low and aromatic and cyclic residues were present. This Cd-linking compound seems to be unique, since it was very different both from metallothioneins and chelatins isolated by other protozoa. The protective role of these chelating Proteins is discussed.
Nicole Dhainaut-courtois - One of the best experts on this subject based on the ideXlab platform.
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Amino acid sequence of the small Cadmium-Binding Protein (MP II) from Nereis diversicolor (annelida, polychaeta) : evidence for a myohemerythrin structure
European journal of biochemistry, 1993Co-Authors: Sylvain Demuynck, Roel C. Van Der Schors, Nicole Dhainaut-courtoisAbstract:The primary sequence of the low-molecular-mass Cadmium-Binding Protein metalloProtein II of Nereis diversicolor (Hediste diversicolor, recent denomination) has been determined. This Protein is composed of 119 amino acids and has 80.8% identity with the N. diversicolor myohemerythrin [Takagi, T. & Cox, J. A. (1991) FEBS Lett. 285, 25–27]. The fact that iron, which normally binds to myohemerythrin, is not found to be associated with the Cadmium-Binding Protein metalloProtein II in Cadmium-exposed animals could be the result of the complete abolition of the iron-Binding capacity of the Protein due to the Binding of Cadmium.
Avianna Zhukovskaya - One of the best experts on this subject based on the ideXlab platform.
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The role of the Cadmium-Binding Protein response of the digestive gland of the Yesso scallop Mizuhopecten yessoensis (Jay, 1857) for marine environmental assessments
Environmental Science and Pollution Research, 2021Co-Authors: Avianna Zhukovskaya, Vladimir Goryachev, Maksim Zakhartsev, Viktor ChelominAbstract:The ability of Pectinidae to accumulate heavy metals and store them in their tissues allows the use of scallops for biomonitoring marine pollution. High molecular weight metallothionein (MT)-like Proteins (MTlps) play a central role in this process. Two major MTlps (72 and 43 kDa) have been identified in the digestive glands of Mizuhopecten yessoensis (Yesso scallop). These Proteins have a very high affinity for the heavy metals Cadmium, cobalt, and caesium. Additionally, these Proteins can be deposited in large quantities in the digestive glands of this mollusc. It has been shown that 72 kDa MTlp is the main stress-response Protein in areas polluted with Cadmium or radioactive metals. Monitoring the amounts of MTlps in the digestive glands of the scallop M. yessoensis in areas with different anthropogenic pollutants has shown that these Proteins are reliable biological markers of heavy-metal pollution in the marine environment.
