The Experts below are selected from a list of 12120 Experts worldwide ranked by ideXlab platform
Katherine Anne Comtois - One of the best experts on this subject based on the ideXlab platform.
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collaborative assessment and management of suicidality Cams feasibility trial for next day appointment services
Depression and Anxiety, 2011Co-Authors: David A Jobes, Katherine Anne Comtois, Stephen S Oconnor, David C Atkins, Karin Janis, Chloe E Chessen, Sara J Landes, Anna HolenAbstract:BACKGROUND: Despite the ubiquity of suicidality in behavioral health settings, empirically supported interventions for suicidality are surprisingly rare. Given the importance of resolving suicidality and therapists' anxieties about treating suicidal patients, there is a clear need for innovative services and clinical approaches. The purpose of the current study was an attempt to address some of these needs by examining the feasibility and use of a new intervention called the "Collaborative Assessment and Management of Suicidality" (Cams) within a "Next-Day Appointment" (NDA) outpatient treatment setting. METHODS: As part of a larger feasibility study, n = 32 suicidal patients were randomly assigned to Cams care versus Enhanced Care as Usual (E-CAU) in an outpatient crisis intervention setting attached to a safety net hospital. Intent to treat suicidal patients were seen and assessed before, during, and after treatment (with follow-up assessments conducted at 2, 4, 6, and 12 months). RESULTS: The feasibility of using Cams in the NDA setting was clear; both groups appeared to initially benefit from their respective treatments in terms of decreased suicidal ideation and overall symptom distress. Although patients rated both treatments favorably, the Cams group had significantly higher satisfaction and better treatment retention than E-CAU. At 12 months post-treatment, Cams patients showed significantly better and sustained reductions in suicidal ideation, overall symptom distress, and increased hope in comparison to E-CAU patients. CONCLUSIONS: Cams was both feasible in this NDA setting and effective in treating suicidal ideation, distress, and hopelessness (particularly at 12 months followup).
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collaborative assessment and management of suicidality Cams feasibility trial for next day appointment services
Depression and Anxiety, 2011Co-Authors: David A Jobes, Katherine Anne Comtois, Stephen S Oconnor, David C Atkins, Karin Janis, Chloe E Chessen, Sara J Landes, Anna HolenAbstract:Background: Despite the ubiquity of suicidality in behavioral health settings, empirically supported interventions for suicidality are surprisingly rare. Given the importance of resolving suicidality and therapists’ anxieties about treating suicidal patients, there is a clear need for innovative services and clinical approaches. The purpose of the current study was an attempt to address some of these needs by examining the feasibility and use of a new intervention called the ‘‘Collaborative Assessment and Management of Suicidality’’ (Cams) within a ‘‘Next-Day Appointment’’ (NDA) outpatient treatment setting. Methods: As part of a larger feasibility study, n 532 suicidal patients were randomly assigned to Cams care versus Enhanced Care as Usual (E-CAU) in an outpatient crisis intervention setting attached to a safety net hospital. Intent to treat suicidal patients were seen and assessed before, during, and after treatment (with follow-up assessments conducted at 2, 4, 6, and 12 months). Results: The feasibility of using Cams in the NDA setting was clear; both groups appeared to initially benefit from their respective treatments in terms of decreased suicidal ideation and overall symptom distress. Although patients rated both treatments favorably, the Cams group had significantly higher satisfaction and better treatment retention than E-CAU. At 12 months posttreatment, Cams patients showed significantly better and sustained reductions in suicidal ideation, overall symptom distress, and increased hope in comparison to ECAU patients. Conclusions: Cams was both feasible in this NDA setting and effective in treating suicidal ideation, distress, and hopelessness (particularly at 12 months followup). Depression and Anxiety 28:963–972, 2011. r 2011 Wiley
Anna Holen - One of the best experts on this subject based on the ideXlab platform.
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collaborative assessment and management of suicidality Cams feasibility trial for next day appointment services
Depression and Anxiety, 2011Co-Authors: David A Jobes, Katherine Anne Comtois, Stephen S Oconnor, David C Atkins, Karin Janis, Chloe E Chessen, Sara J Landes, Anna HolenAbstract:BACKGROUND: Despite the ubiquity of suicidality in behavioral health settings, empirically supported interventions for suicidality are surprisingly rare. Given the importance of resolving suicidality and therapists' anxieties about treating suicidal patients, there is a clear need for innovative services and clinical approaches. The purpose of the current study was an attempt to address some of these needs by examining the feasibility and use of a new intervention called the "Collaborative Assessment and Management of Suicidality" (Cams) within a "Next-Day Appointment" (NDA) outpatient treatment setting. METHODS: As part of a larger feasibility study, n = 32 suicidal patients were randomly assigned to Cams care versus Enhanced Care as Usual (E-CAU) in an outpatient crisis intervention setting attached to a safety net hospital. Intent to treat suicidal patients were seen and assessed before, during, and after treatment (with follow-up assessments conducted at 2, 4, 6, and 12 months). RESULTS: The feasibility of using Cams in the NDA setting was clear; both groups appeared to initially benefit from their respective treatments in terms of decreased suicidal ideation and overall symptom distress. Although patients rated both treatments favorably, the Cams group had significantly higher satisfaction and better treatment retention than E-CAU. At 12 months post-treatment, Cams patients showed significantly better and sustained reductions in suicidal ideation, overall symptom distress, and increased hope in comparison to E-CAU patients. CONCLUSIONS: Cams was both feasible in this NDA setting and effective in treating suicidal ideation, distress, and hopelessness (particularly at 12 months followup).
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collaborative assessment and management of suicidality Cams feasibility trial for next day appointment services
Depression and Anxiety, 2011Co-Authors: David A Jobes, Katherine Anne Comtois, Stephen S Oconnor, David C Atkins, Karin Janis, Chloe E Chessen, Sara J Landes, Anna HolenAbstract:Background: Despite the ubiquity of suicidality in behavioral health settings, empirically supported interventions for suicidality are surprisingly rare. Given the importance of resolving suicidality and therapists’ anxieties about treating suicidal patients, there is a clear need for innovative services and clinical approaches. The purpose of the current study was an attempt to address some of these needs by examining the feasibility and use of a new intervention called the ‘‘Collaborative Assessment and Management of Suicidality’’ (Cams) within a ‘‘Next-Day Appointment’’ (NDA) outpatient treatment setting. Methods: As part of a larger feasibility study, n 532 suicidal patients were randomly assigned to Cams care versus Enhanced Care as Usual (E-CAU) in an outpatient crisis intervention setting attached to a safety net hospital. Intent to treat suicidal patients were seen and assessed before, during, and after treatment (with follow-up assessments conducted at 2, 4, 6, and 12 months). Results: The feasibility of using Cams in the NDA setting was clear; both groups appeared to initially benefit from their respective treatments in terms of decreased suicidal ideation and overall symptom distress. Although patients rated both treatments favorably, the Cams group had significantly higher satisfaction and better treatment retention than E-CAU. At 12 months posttreatment, Cams patients showed significantly better and sustained reductions in suicidal ideation, overall symptom distress, and increased hope in comparison to ECAU patients. Conclusions: Cams was both feasible in this NDA setting and effective in treating suicidal ideation, distress, and hopelessness (particularly at 12 months followup). Depression and Anxiety 28:963–972, 2011. r 2011 Wiley
Kathryn E Uhrich - One of the best experts on this subject based on the ideXlab platform.
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cationic amphiphiles against gardnerella vaginalis resistant strains and bacterial vaginosis associated pathogens
Pathogens and Disease, 2019Co-Authors: Richard Weeks, Alysha Moretti, Shuang Song, Kathryn E Uhrich, Andrey V Karlyshev, Michael L ChikindasAbstract:Antibiotic resistance and infection recurrence are critical issues in treating bacterial vaginosis, the most common vaginal disorder in women of reproductive age. Novel alternatives to traditional antibiotics, such as peptidomimetics, have the potential to address this challenge. Previously, two series of cationic amphiphiles (Cams) were developed with both hydrophilic head groups and non-polar domains, giving them the ability to self-assemble into supramolecular nanostructures with membrane-lytic properties. Those Cams were shown to be effective against biofilms of Gardnerella vaginalis while preserving the commensal microbiota. Two new series of Cams were designed with varying levels of flexibility between the hydrophilic head groups and the hydrophobic domains. Activities against the vaginal pathogen G. vaginalis ranged from 1.3 to 18.5 µM, while the tested vaginal lactobacilli were significantly more tolerant of Cams, with minimal inhibitory concentration values as high as 208 µM. Minimal biofilm bactericidal concentrations of the tested Cams ranged from 21.47 to <388.3 µM, and were lowest against resistant forms of G. vaginalis, while Lactobacillus biofilms were tolerant of concentrations ≥687 µM. Safety aspects of the Cams were also investigated, and they were found to be safe for use against vaginal ectocervical tissue.
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cationic amphiphiles with specificity against gram positive and gram negative bacteria chemical composition and architecture combat bacterial membranes
Langmuir, 2019Co-Authors: Alysha Moretti, Richard Weeks, Kathryn E Uhrich, Michael L ChikindasAbstract:Small-molecule cationic amphiphiles (Cams) were designed to combat the rapid rise in drug-resistant bacteria. Cams were designed to target and compromise the structural integrity of bacteria membranes, leading to cell rupture and death. Discrete structural features of Cams were varied, and structure–activity relationship studies were performed to guide the rational design of potent antimicrobials with desirable selectivity and cytocompatibility profiles. In particular, the effects of cationic conformational flexibility, hydrophobic domain flexibility, and hydrophobic domain architecture were evaluated. Their influence on antimicrobial efficacy in Gram-positive and Gram-negative bacteria was determined, and their safety profiles were established by assessing their impact on mammalian cells. All Cams have a potent activity against bacteria, and hydrophobic domain rigidity and branched architecture contribute to specificity. The insights gained from this project will aid in the optimization of CAm structures.
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self assembled cationic amphiphiles as antimicrobial peptides mimics role of hydrophobicity linkage type and assembly state
Nanomedicine: Nanotechnology Biology and Medicine, 2017Co-Authors: Yingyue Zhang, Michael L Chikindas, Ammar Algburi, Ning Wang, Vladyslav Kholodovych, Drym O Oh, Kathryn E UhrichAbstract:Abstract Inspired by high promise using naturally occurring antimicrobial peptides (AMPs) to treat infections caused by antimicrobial-resistant bacteria, cationic amphiphiles (Cams) were strategically designed as synthetic mimics to overcome associated limitations, including high manufacture cost and low metabolic stability. Cams with facially amphiphilic conformation were expected to demonstrate membrane-lytic properties and thus reduce tendency of resistance development. By systematically tuning the hydrophobicity, Cams with optimized compositions exhibited potent broad-spectrum antimicrobial activity (with minimum inhibitory concentrations in low μg/mL range) as well as negligible hemolytic activity. Electron microscope images revealed the morphological and ultrastructure changes of bacterial membranes induced by CAm treatment and validated their membrane-disrupting mechanism. Additionally, an all-atom molecular dynamics simulation was employed to understand the CAm-membrane interaction on molecular level. This study shows that these Cams can serve as viable scaffolds for designing next generation of AMP mimics as antimicrobial alternatives to combat drug-resistant pathogens.
Sara J Landes - One of the best experts on this subject based on the ideXlab platform.
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collaborative assessment and management of suicidality Cams feasibility trial for next day appointment services
Depression and Anxiety, 2011Co-Authors: David A Jobes, Katherine Anne Comtois, Stephen S Oconnor, David C Atkins, Karin Janis, Chloe E Chessen, Sara J Landes, Anna HolenAbstract:BACKGROUND: Despite the ubiquity of suicidality in behavioral health settings, empirically supported interventions for suicidality are surprisingly rare. Given the importance of resolving suicidality and therapists' anxieties about treating suicidal patients, there is a clear need for innovative services and clinical approaches. The purpose of the current study was an attempt to address some of these needs by examining the feasibility and use of a new intervention called the "Collaborative Assessment and Management of Suicidality" (Cams) within a "Next-Day Appointment" (NDA) outpatient treatment setting. METHODS: As part of a larger feasibility study, n = 32 suicidal patients were randomly assigned to Cams care versus Enhanced Care as Usual (E-CAU) in an outpatient crisis intervention setting attached to a safety net hospital. Intent to treat suicidal patients were seen and assessed before, during, and after treatment (with follow-up assessments conducted at 2, 4, 6, and 12 months). RESULTS: The feasibility of using Cams in the NDA setting was clear; both groups appeared to initially benefit from their respective treatments in terms of decreased suicidal ideation and overall symptom distress. Although patients rated both treatments favorably, the Cams group had significantly higher satisfaction and better treatment retention than E-CAU. At 12 months post-treatment, Cams patients showed significantly better and sustained reductions in suicidal ideation, overall symptom distress, and increased hope in comparison to E-CAU patients. CONCLUSIONS: Cams was both feasible in this NDA setting and effective in treating suicidal ideation, distress, and hopelessness (particularly at 12 months followup).
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collaborative assessment and management of suicidality Cams feasibility trial for next day appointment services
Depression and Anxiety, 2011Co-Authors: David A Jobes, Katherine Anne Comtois, Stephen S Oconnor, David C Atkins, Karin Janis, Chloe E Chessen, Sara J Landes, Anna HolenAbstract:Background: Despite the ubiquity of suicidality in behavioral health settings, empirically supported interventions for suicidality are surprisingly rare. Given the importance of resolving suicidality and therapists’ anxieties about treating suicidal patients, there is a clear need for innovative services and clinical approaches. The purpose of the current study was an attempt to address some of these needs by examining the feasibility and use of a new intervention called the ‘‘Collaborative Assessment and Management of Suicidality’’ (Cams) within a ‘‘Next-Day Appointment’’ (NDA) outpatient treatment setting. Methods: As part of a larger feasibility study, n 532 suicidal patients were randomly assigned to Cams care versus Enhanced Care as Usual (E-CAU) in an outpatient crisis intervention setting attached to a safety net hospital. Intent to treat suicidal patients were seen and assessed before, during, and after treatment (with follow-up assessments conducted at 2, 4, 6, and 12 months). Results: The feasibility of using Cams in the NDA setting was clear; both groups appeared to initially benefit from their respective treatments in terms of decreased suicidal ideation and overall symptom distress. Although patients rated both treatments favorably, the Cams group had significantly higher satisfaction and better treatment retention than E-CAU. At 12 months posttreatment, Cams patients showed significantly better and sustained reductions in suicidal ideation, overall symptom distress, and increased hope in comparison to ECAU patients. Conclusions: Cams was both feasible in this NDA setting and effective in treating suicidal ideation, distress, and hopelessness (particularly at 12 months followup). Depression and Anxiety 28:963–972, 2011. r 2011 Wiley
Alfred L George - One of the best experts on this subject based on the ideXlab platform.
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divergent regulation of ryanodine receptor 2 calcium release channels by arrhythmogenic human calmodulin missense mutantsnovelty and significance
Circulation Research, 2014Co-Authors: Hyun Seok Hwang, Kafa Walweel, Michela Faggioni, Laetitia Pereira, Florentin R Nitu, Derek R. Laver, Walter J. Chazin, Christopher N. Johnson, Yi Yang, Alfred L GeorgeAbstract:Rationale: Calmodulin (CaM) mutations are associated with an autosomal dominant syndrome of ventricular arrhythmia and sudden death that can present with divergent clinical features of catecholaminergic polymorphic ventricular tachycardia (CPVT) or long QT syndrome (LQTS). CaM binds to and inhibits ryanodine receptor (RyR2) Ca release channels in the heart, but whether arrhythmogenic CaM mutants alter RyR2 function is not known. Objective: To gain mechanistic insight into how human CaM mutations affect RyR2 Ca channels. Methods and Results: We studied recombinant CaM mutants associated with CPVT (N54I and N98S) or LQTS (D96V, D130G, and F142L). As a group, all LQTS-associated CaM mutants (LQTS-Cams) exhibited reduced Ca affinity, whereas CPVT-associated CaM mutants (CPVT-Cams) had either normal or modestly lower Ca affinity. In permeabilized ventricular myocytes, CPVT-Cams at a physiological intracellular concentration (100 nmol/L) promoted significantly higher spontaneous Ca wave and spark activity, a typical cellular phenotype of CPVT. Compared with wild-type CaM, CPVT-Cams caused greater RyR2 single-channel open probability and showed enhanced binding affinity to RyR2. Even a 1:8 mixture of CPVT-CaM:wild-type-CaM activated Ca waves, demonstrating functional dominance. In contrast, LQTS-Cams did not promote Ca waves and exhibited either normal regulation of RyR2 single channels (D96V) or lower RyR2-binding affinity (D130G and F142L). None of the CaM mutants altered Ca/CaM binding to CaM-kinase II. Conclusions: A small proportion of CPVT-CaM is sufficient to evoke arrhythmogenic Ca disturbances, whereas LQTS-Cams do not. Our findings explain the clinical presentation and autosomal dominant inheritance of CPVT-CaM mutations and suggest that RyR2 interactions are unlikely to explain arrhythmogenicity of LQTS-CaM mutations. # Novelty and Significance {#article-title-50}
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divergent regulation of ryanodine receptor 2 calcium release channels by arrhythmogenic human calmodulin missense mutants
Circulation Research, 2014Co-Authors: Hyun Seok Hwang, Kafa Walweel, Michela Faggioni, Laetitia Pereira, Florentin R Nitu, Derek R. Laver, Walter J. Chazin, Christopher N. Johnson, Yi Yang, Alfred L GeorgeAbstract:Rationale: Calmodulin (CaM) mutations are associated with an autosomal dominant syndrome of ventricular arrhythmia and sudden death that can present with divergent clinical features of catecholaminergic polymorphic ventricular tachycardia (CPVT) or long QT syndrome (LQTS). CaM binds to and inhibits ryanodine receptor (RyR2) Ca release channels in the heart, but whether arrhythmogenic CaM mutants alter RyR2 function is not known. Objective: To gain mechanistic insight into how human CaM mutations affect RyR2 Ca channels. Methods and Results: We studied recombinant CaM mutants associated with CPVT (N54I and N98S) or LQTS (D96V, D130G, and F142L). As a group, all LQTS-associated CaM mutants (LQTS-Cams) exhibited reduced Ca affinity, whereas CPVT-associated CaM mutants (CPVT-Cams) had either normal or modestly lower Ca affinity. In permeabilized ventricular myocytes, CPVT-Cams at a physiological intracellular concentration (100 nmol/L) promoted significantly higher spontaneous Ca wave and spark activity, a typical cellular phenotype of CPVT. Compared with wild-type CaM, CPVT-Cams caused greater RyR2 single-channel open probability and showed enhanced binding affinity to RyR2. Even a 1:8 mixture of CPVT-CaM:wild-type-CaM activated Ca waves, demonstrating functional dominance. In contrast, LQTS-Cams did not promote Ca waves and exhibited either normal regulation of RyR2 single channels (D96V) or lower RyR2-binding affinity (D130G and F142L). None of the CaM mutants altered Ca/CaM binding to CaM-kinase II. Conclusions: A small proportion of CPVT-CaM is sufficient to evoke arrhythmogenic Ca disturbances, whereas LQTS-Cams do not. Our findings explain the clinical presentation and autosomal dominant inheritance of CPVT-CaM mutations and suggest that RyR2 interactions are unlikely to explain arrhythmogenicity of LQTS-CaM mutations.
