The Experts below are selected from a list of 474 Experts worldwide ranked by ideXlab platform
Neus Teixidó - One of the best experts on this subject based on the ideXlab platform.
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verifying the biocontrol activity of novel film forming formulations of Candida Sake cpa 1 resilience in relation to environmental factors rainfall episodes and control of botrytis cinerea on different hosts
Journal of the Science of Food and Agriculture, 2019Co-Authors: Adria Carbo, Neus Teixidó, J Usall, R TorresAbstract:BACKGROUND The efficacy of Candida Sake CPA-1 as a biocontrol agent against several diseases has been studied since it was isolated 20 years ago. However, it was only recently that two suitable and effective film-forming formulations based on potato starch and maltodextrins were developed using the fluidized-bed spray-drying system. The present work aimed to confirm the capability of both novel formulations by testing their resilience on grapes at different temperatures (0, 22, and 30 °C), relative humidities (40% and 85%), and simulated rainfall levels. Another objective was to examine the control of Botrytis cinerea in different hosts. RESULTS The CPA-1 cells from both dried formulations survived better than the liquid formulation on grapes stored at 0 and 22 °C regardless of the relative humidity. After simulated rainfall, potato starch formulation achieved significantly higher populations than maltodextrin formulation, although the highest reduction was -1.6 log N N0-1 . A positive effect of cell establishment prior to the simulated rainfall was shown, and recovered cells from the potato starch formulation were significantly higher after 72 h of cell establishment. Finally, both formulations reduced the incidence and severity of B. cinerea on pears, apples, and tomatoes. CONCLUSION The potential of these novel film-forming formulations of C. Sake CPA-1 was verified. The resilience of formulated C. Sake was better than the commercialized liquid formulation, the adherence of the formulations to the grapes improved after an establishment period prior to rain exposure, and the control of B. cinerea was verified in a wider range of hosts. © 2019 Society of Chemical Industry.
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Shelf life improvement of the biocontrol agent Candida Sake CPA-1 by suitable package and storage conditions
BioControl, 2019Co-Authors: Adria Carbo, Josep Usall, Neus Teixidó, Cristina Solsona, Rosario TorresAbstract:Agrochemical companies are increasing their interest in the production of biopesticides based on biocontrol agents (BCAs), mainly due to the need to reduce the synthetic fungicide application and the consequent benefits for the environment and for human health. The efficacy of the BCA Candida Sake CPA-1 has been demonstrated against the main postharvest diseases on pome fruit, and B. cinerea and sour rot in grapes control on field. Recently, two effective fluidised-bed spray-dried formulations based on C. Sake CPA-1 and biodegradable compounds were described as an effective biocontrol product. However, the maintenance of the effectiveness and survival of these formulations during a long shelf life is a crucial aspect to the success of its marketing. For this reason, the aim of this study was to select the best packaging and storage conditions to maintain a long shelf life of two fluidised-bed spray-dried C. Sake CPA-1 formulations. Viability and a _w (water activity) of CPA-1 formulations stored in different packages (bottles and bags), temperatures (− 20 °C and 4 °C) and atmospheric conditions (vacuum and air) were tested for 22 months. Both formulations maintained their initial viability (around 4 × 10^9 CFU ml^−1) when they were stored at − 20 °C regardless of the packages or atmospheric conditions. In contrast, the viability of CPA-1 formulations stored at 4 °C differed between bags and bottles. Both formulations could be stored for at least 21 months at − 20 °C, and only one formulation could achieve a one-year shelf life stored in bottles at 4 °C. C. Sake CPA-1 formulations stored for 12 months at the most suitable conditions significantly reduced B. cinerea on grapes by 25 to 56%.
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novel film forming formulations of the biocontrol agent Candida Sake cpa 1 biocontrol efficacy and performance at field conditions in organic wine grapes
Pest Management Science, 2019Co-Authors: Adria Carbo, Josep Usall, R Torres, A Marin, Amparo Chiralt, Neus TeixidóAbstract:This is the peer reviewed version of the following article: Carbo, A., Torres, R., Usall, J., Marin, A., Chiralt, A. and Teixido, N. (2019), Novel film?forming formulations of the biocontrol agent Candida Sake CPA?1: biocontrol efficacy and performance at field conditions in organic wine grapes. Pest. Manag. Sci., 75: 959-968, which has been published in final form at https://doi.org/10.1002/ps.5200. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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impact of climate change environmental conditions on the resilience of different formulations of the biocontrol agent Candida Sake cpa 1 on grapes
Letters in Applied Microbiology, 2018Co-Authors: Adria Carbo, Josep Usall, Neus Teixidó, R Torres, Angel Medina, Naresh MaganAbstract:Biocontrol agents have become components of integrated crop protection systems for controlling economically important fungal pathogens. Candida Sake CPA-1 is a biocontrol agent of fungal pathogens of fruits, both pre- and post-harvest. While the efficacy of different formulations have been examined previously, few studies have considered the resilience of different formulations under changing climatic conditions of elevated temperature, drought stress and increased atmospheric CO2 . This study examined the effect of (a) temperature × RH × elevated CO2 (400 vs 1000 ppm) on the temporal establishment and viability of two dry and one liquid C. Sake CPA-1 formulations on grape berry surfaces; (b) temperature stress (25 vs 35°C); and (c) elevated CO2 levels. Results indicated that temperature, RH and CO2 concentration influenced the establishment and viability of the formulations but there was no significant difference between formulations. For the combined three-component factors, increased temperature (35°C) and lower RH (40%) reduced the viable populations on grapes. The interaction with elevated CO2 improved the establishment of viable populations of the formulations tested. Viable populations greater than Log 4 CFUs per g were recovered from the grape surfaces suggesting that these had conserved resilience for control of Botrytis rot in grapes. SIGNIFICANCE AND IMPACT OF THE STUDY The interaction between environmental factors that are expected to occur in response to climate change (CC) will have a significant impact on food security and availability. Little information exists on how elevated temperature, drought stress and increased CO2 will have on the efficacy of biocontrol agents. The impact of these factors on the viability of different formulations of the biocontrol yeast Candida Sake on the surface of grapes berries was evaluated for the first time. Such knowledge is critical for projecting the efficacy of biocontrol under climate change conditions and to identify formulations that have the necessary resilience to perform under CC conditions.
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Fluidised-bed spray-drying formulations of Candida Sake CPA-1 by adding biodegradable coatings to enhance their survival under stress conditions
Applied Microbiology and Biotechnology, 2017Co-Authors: Adria Carbo, Carles Solsona, Rosario Torres, Neus TeixidóAbstract:The biocontrol agent Candida Sake CPA-1 has demonstrated to be effective against several diseases on fruit. However, for application of CPA-1 under field conditions, it was necessary to mix it with a food coating to improve survival under stress conditions, as well as adherence and distribution on fruit surfaces. The objective of this study was to obtain a more competitive formulation under field conditions to be applied independently of any product. To achieve this purpose, the drying process of CPA-1 by a fluidised-bed spray-drying system together with biodegradable coatings was optimised. This approach is novel for the drying system used and the formulation obtained which was able to form a film or coating on fruit surfaces. Several substances were tested as carriers and binders, and drying temperature was optimised. The addition of protective compounds was also tested to improve survival of CPA-1 during the dehydration process. Product shelf life, biocontrol efficacy on grapes against Botrytis cinerea, and the improvement of C. Sake behaviour under stress conditions were tested. The optimal temperature of drying was 55 °C and two formulations that were able to develop a coating on fruit surfaces were obtained. One of the formulations was created by using a combination of native and pregelatinised potato starch; the other formulation was obtained using maltodextrin and by adding skimmed milk and sucrose as protectant compounds. The formulated products reduced the incidence and severity of B. cinerea, and CPA-1 survival rate was increased under stress conditions of temperature and humidity.
Josep Usall - One of the best experts on this subject based on the ideXlab platform.
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Shelf life improvement of the biocontrol agent Candida Sake CPA-1 by suitable package and storage conditions
BioControl, 2019Co-Authors: Adria Carbo, Josep Usall, Neus Teixidó, Cristina Solsona, Rosario TorresAbstract:Agrochemical companies are increasing their interest in the production of biopesticides based on biocontrol agents (BCAs), mainly due to the need to reduce the synthetic fungicide application and the consequent benefits for the environment and for human health. The efficacy of the BCA Candida Sake CPA-1 has been demonstrated against the main postharvest diseases on pome fruit, and B. cinerea and sour rot in grapes control on field. Recently, two effective fluidised-bed spray-dried formulations based on C. Sake CPA-1 and biodegradable compounds were described as an effective biocontrol product. However, the maintenance of the effectiveness and survival of these formulations during a long shelf life is a crucial aspect to the success of its marketing. For this reason, the aim of this study was to select the best packaging and storage conditions to maintain a long shelf life of two fluidised-bed spray-dried C. Sake CPA-1 formulations. Viability and a _w (water activity) of CPA-1 formulations stored in different packages (bottles and bags), temperatures (− 20 °C and 4 °C) and atmospheric conditions (vacuum and air) were tested for 22 months. Both formulations maintained their initial viability (around 4 × 10^9 CFU ml^−1) when they were stored at − 20 °C regardless of the packages or atmospheric conditions. In contrast, the viability of CPA-1 formulations stored at 4 °C differed between bags and bottles. Both formulations could be stored for at least 21 months at − 20 °C, and only one formulation could achieve a one-year shelf life stored in bottles at 4 °C. C. Sake CPA-1 formulations stored for 12 months at the most suitable conditions significantly reduced B. cinerea on grapes by 25 to 56%.
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novel film forming formulations of the biocontrol agent Candida Sake cpa 1 biocontrol efficacy and performance at field conditions in organic wine grapes
Pest Management Science, 2019Co-Authors: Adria Carbo, Josep Usall, R Torres, A Marin, Amparo Chiralt, Neus TeixidóAbstract:This is the peer reviewed version of the following article: Carbo, A., Torres, R., Usall, J., Marin, A., Chiralt, A. and Teixido, N. (2019), Novel film?forming formulations of the biocontrol agent Candida Sake CPA?1: biocontrol efficacy and performance at field conditions in organic wine grapes. Pest. Manag. Sci., 75: 959-968, which has been published in final form at https://doi.org/10.1002/ps.5200. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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impact of climate change environmental conditions on the resilience of different formulations of the biocontrol agent Candida Sake cpa 1 on grapes
Letters in Applied Microbiology, 2018Co-Authors: Adria Carbo, Josep Usall, Neus Teixidó, R Torres, Angel Medina, Naresh MaganAbstract:Biocontrol agents have become components of integrated crop protection systems for controlling economically important fungal pathogens. Candida Sake CPA-1 is a biocontrol agent of fungal pathogens of fruits, both pre- and post-harvest. While the efficacy of different formulations have been examined previously, few studies have considered the resilience of different formulations under changing climatic conditions of elevated temperature, drought stress and increased atmospheric CO2 . This study examined the effect of (a) temperature × RH × elevated CO2 (400 vs 1000 ppm) on the temporal establishment and viability of two dry and one liquid C. Sake CPA-1 formulations on grape berry surfaces; (b) temperature stress (25 vs 35°C); and (c) elevated CO2 levels. Results indicated that temperature, RH and CO2 concentration influenced the establishment and viability of the formulations but there was no significant difference between formulations. For the combined three-component factors, increased temperature (35°C) and lower RH (40%) reduced the viable populations on grapes. The interaction with elevated CO2 improved the establishment of viable populations of the formulations tested. Viable populations greater than Log 4 CFUs per g were recovered from the grape surfaces suggesting that these had conserved resilience for control of Botrytis rot in grapes. SIGNIFICANCE AND IMPACT OF THE STUDY The interaction between environmental factors that are expected to occur in response to climate change (CC) will have a significant impact on food security and availability. Little information exists on how elevated temperature, drought stress and increased CO2 will have on the efficacy of biocontrol agents. The impact of these factors on the viability of different formulations of the biocontrol yeast Candida Sake on the surface of grapes berries was evaluated for the first time. Such knowledge is critical for projecting the efficacy of biocontrol under climate change conditions and to identify formulations that have the necessary resilience to perform under CC conditions.
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effect of different coating forming agents on the efficacy of the biocontrol agent Candida Sake cpa 1 for control of botrytis cinerea on grapes
Biological Control, 2016Co-Authors: A Marin, Josep Usall, R Torres, Amparo Chiralt, Lorena Atares, Maite Chafer, Neus TeixidóAbstract:Multiple formulations of known biocontrol agent (BCA) Candida Sake, containing different coating-forming polymers and surfactants were tested at different polymer:BCA ratios, in order to improve control of Botrytis cinerea on grapes. The BCA cell viability on the grape surface was analyzed and reduction in disease incidence and severity was determined. Coating-forming solids improved the survival and efficacy of C. Sake as a BCA against B. cinerea, depending on the polymer type and ratio. The incorporation of surfactants did not improve survival or disease control, although they promoted a better cell dispersion on the grape surface. Cell growth of the antagonist during incubation led to the formation of aggregates, even when surfactants were present. Sodium caseinate and starch were the most suitable polymers to formulate C. Sake preparations to obtain coating-forming systems with this BCA and to increase its survival and efficacy at the minimum economic cost of the ingredients.
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survival of the biological control agent Candida Sake cpa 1 on grapes under the influence of abiotic factors
Journal of Applied Microbiology, 2014Co-Authors: C Calvogarrido, Josep Usall, I Vinas, M Rodriguezromera, M C Ramos, Neus TeixidóAbstract:Aims As reliability of preharvest applications of biological control agents (BCAs) to control fruit pathogens is highly dependent on the survival of the selected organism, this study aimed to describe the population dynamics of the yeast-BCA Candida Sake (Saito & Ota) CPA-1 on grape berries under the effect of abiotic factors such as temperature, relative humidity, sunlight and rainfall. Methods and Results Candida Sake (5 × 107 CFU ml−1), combined with different concentrations of the food additive Fungicover®, was applied on grape berry clusters. Treated clusters were then exposed to abiotic factors in field or laboratory conditions, recovering populations to describe C. Sake population dynamics. The addition of Fungicover significantly increased C. Sake multiplication under optimal growth conditions and improved survival under fluctuating abiotic factors. After field applications, significant differences in populations on grape bunches exposed or covered by fine foliage were detected. Simulated rainfall washed off C. Sake populations by 0·6–0·9 log units after 20 mm of rain volume. Allowing populations to establish for 24 h or more, prior to a rain event, persistence on grape berries significantly increased and the effect of rain intensity was not observable. Conclusions Candida Sake demonstrated high survival ability under unfavourable environmental conditions and persistence under intense rain. The study evidenced the importance of the first period just after application for C. Sake survival on grape tissues and also the protective effect of the additive Fungicover. Significance and Impact of the Study This research provides new information on the survival of C. Sake under field conditions and its practical implications for recommending timing of spray with this antagonist. Our results could be useful for other yeast antagonists applied before harvest. This work, for the first time, defines population dynamics of a yeast BCA using simulated rainfall.
I Vinas - One of the best experts on this subject based on the ideXlab platform.
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biological control of botrytis bunch rot in atlantic climate vineyards with Candida Sake cpa 1 and its survival under limiting conditions of temperature and humidity
Biological Control, 2014Co-Authors: C Calvogarrido, Neus Teixidó, I Vinas, J Usall, J Roudet, M FermaudAbstract:Abstract Candida Sake CPA-1 is an antagonistic yeast that has previously been shown to effectively control Botrytis bunch rot in grapes. The efficacy of biological control agents is dependent on their survival, which may also depend on climatic conditions. However, few studies have evaluated the effect of abiotic factors affecting the survival of biological control agents, such as temperature (T) or relative humidity (RH). In this study, efficacy of C. Sake (5 × 10 7 CFU mL − 1 ), which was applied with the additive Fungicover (FC; 50 g L − 1 ), was tested against BBR in the laboratory and in field trials under the Atlantic climate conditions of the Bordeaux region (France). The study also evaluated the survival of C. Sake under T and RH regimes simulated in climatic chambers. Two or five applications of C. Sake plus FC during the growing season significantly reduced BBR severity at harvest by 48% and 82%, respectively, when compared to the control. Similar reductions were achieved after inoculation with selected virulent Botrytis cinerea strains (75% compared to control) in laboratory experiments. C. Sake populations showed minimal decreases between field applications and were favored by simulated Atlantic climate conditions. The survival pattern of C. Sake exposed to 40 and 45 °C combined with 30% and 100% of RH was described, demonstrating a sharp decrease during the first 24 h. Allowing 48 h for C. Sake to incubate and become established on fruits prior to the exposure to 40 °C and 30% RH increased survival ( P C. Sake plus FC under favorable climatic conditions for BBR development, while survival studies may help to improve the survival and efficacy of yeast BCAs, such as C. Sake CPA-1.
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survival of the biological control agent Candida Sake cpa 1 on grapes under the influence of abiotic factors
Journal of Applied Microbiology, 2014Co-Authors: C Calvogarrido, Josep Usall, I Vinas, M Rodriguezromera, M C Ramos, Neus TeixidóAbstract:Aims As reliability of preharvest applications of biological control agents (BCAs) to control fruit pathogens is highly dependent on the survival of the selected organism, this study aimed to describe the population dynamics of the yeast-BCA Candida Sake (Saito & Ota) CPA-1 on grape berries under the effect of abiotic factors such as temperature, relative humidity, sunlight and rainfall. Methods and Results Candida Sake (5 × 107 CFU ml−1), combined with different concentrations of the food additive Fungicover®, was applied on grape berry clusters. Treated clusters were then exposed to abiotic factors in field or laboratory conditions, recovering populations to describe C. Sake population dynamics. The addition of Fungicover significantly increased C. Sake multiplication under optimal growth conditions and improved survival under fluctuating abiotic factors. After field applications, significant differences in populations on grape bunches exposed or covered by fine foliage were detected. Simulated rainfall washed off C. Sake populations by 0·6–0·9 log units after 20 mm of rain volume. Allowing populations to establish for 24 h or more, prior to a rain event, persistence on grape berries significantly increased and the effect of rain intensity was not observable. Conclusions Candida Sake demonstrated high survival ability under unfavourable environmental conditions and persistence under intense rain. The study evidenced the importance of the first period just after application for C. Sake survival on grape tissues and also the protective effect of the additive Fungicover. Significance and Impact of the Study This research provides new information on the survival of C. Sake under field conditions and its practical implications for recommending timing of spray with this antagonist. Our results could be useful for other yeast antagonists applied before harvest. This work, for the first time, defines population dynamics of a yeast BCA using simulated rainfall.
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Candida Sake cpa 1 and other biologically based products as potential control strategies to reduce sour rot of grapes
Letters in Applied Microbiology, 2013Co-Authors: C Calvogarrido, Josep Usall, I Vinas, P A G Elmer, Neus TeixidóAbstract:Sour rot of grapes is becoming increasingly important disease in many winegrowing regions, while consistent chemical or biological control has not been reported. Authors evaluated relative incidence and severity of sour rot in untreated grapevines and the effect of different biologically based treatments on sour rot at harvest. Applications of Candida Sake CPA-1 plus Fungicover , Ulocladium oudemansii and chitosan were carried out in an organic vineyard in Lleida area, Spain, during the 2009 and 2010 growing seasons. At harvest, incidence and severity of sour rot were assessed. Significantly higher incidence and severity of sour rot were observed in untreated plots in 2009, when meteorological conditions after veraison were warmer. All treatments including C. Sake CPA-1 significantly reduced (P < 0 05) severity of sour rot in both seasons, ranging from 40 to 67% compared with the untreated control. Incidence of sour rot was not significantly reduced by any treatment. This study helps to characterize development of sour rot in the dry Mediterranean climate conditions of the experiment, whereas also represents the first report of biological control of sour rot. Treatments with the tested biologically based products are a promising strategy to control sour rot.
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field applications of improved formulations of Candida Sake cpa 1 for control of botrytis cinerea in grapes
Biological Control, 2011Co-Authors: T P Canamas, Josep Usall, I Vinas, C Solsona, R Torres, Neus TeixidóAbstract:Abstract There is increasing interest in the use of biological control agents (BCAs) to replace synthetic fungicides. The aim of this study was to evaluate the effectiveness of combining different formulation strategies on the establishment, survival, persistence and efficacy of Candida Sake CPA-1 applied in the field in order to control Botrytis cinerea in grapes. Different formulations of C. Sake cells were applied at flowering, pea sized berries, veraison and before harvest. In 2005, heat adapted or non-adapted fresh cells of C. Sake were tested. In 2006, these same treatments were tested but, in this case, the C. Sake cells were also formulated in an isotonic solution. The compound Fungicover® (FC) an edible coating was evaluated as a potential additive for C. Sake treatments. Spray applications of different formulations of C. Sake resulted in colonisation of bunches under field conditions and when combined with FC, C. Sake had significantly higher survival rates (up to 50% higher) compared with C. Sake without additive FC. Formulation of C. Sake cells in an isotonic solution combined with FC resulted in Botrytis spp. control similarly than fungicide treatment. The potential of C. Sake for biocontrol of Botrytis bunch rot of grapevine has been demonstrated. FC has a beneficial effect on the BCA, improving the persistence of C. Sake cells on the host and its efficacy to levels comparable to fungicide treatment. It is possible to broaden the spectrum of use of BCAs using different formulation strategies and to thereby develop practical uses under field conditions.
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impact of mild heat treatments on induction of thermotolerance in the biocontrol yeast Candida Sake cpa 1 and viability after spray drying
Journal of Applied Microbiology, 2008Co-Authors: T P Canamas, I Vinas, C Solsona, Naresh Magan, J Usall, Neus TeixidóAbstract:Aims: The objective of this study was to examine the induction of thermotolerance in the biocontrol agent Candida Sake CPA-1 cells by mild heat treatments to enhanced survival of formulations using spray-drying. The possible role of heat-shock proteins (HSPs) biosynthesis in induced thermotolerance and the role of sugars and sugar alcohols were also determined. Methods and Results: Studies were conducted on C. Sake cells grown in molasses medium and exposed to mild temperatures of 30 and 33°C during mid- (16 h), late-exponential (24 h), early- (30 h) and mid-stationary (36 h) growth phases. The effect on viability was determined both before and after spray-drying. Cycloheximide and chloramphenicol were used to examine the role of HSPs and HPLC was used to analyse the accumulation of sugar and sugar alcohols. The results indicate that both temperatures induced thermotolerance in cells of C. Sake. Mild heat-adapted cells at 33°C in the early- or mid-stationary phases had survival values after spray-drying significantly higher (P ≤ 0·05) than nonadapted cells. However, viabilities were not high enough to be considered for commercial use with values up to 17%. HSPs were not implicated in thermotolerance acquired by mild heat-adapted cells as similar viabilities were obtained in the presence of protein inhibitors. Little change was observed in sugar and sugar alcohols with an increase in glucose and arabitol in some treatments. Conclusions: This study suggests that it is possible to induce thermotolerance in biocontrol yeasts such as C. Sake. However, this does not improve survival of cells exposed to spray-drying sufficiently to consider this a suitable formulation method for this biocontrol agent. HSPs, sugars and sugar polyols were not directly responsible for induced thermotolerance in yeast cells. Significance and Impact of the Study: This type of information can be effectively applied to improve the viability of cells in the process of formulation.
R Torres - One of the best experts on this subject based on the ideXlab platform.
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verifying the biocontrol activity of novel film forming formulations of Candida Sake cpa 1 resilience in relation to environmental factors rainfall episodes and control of botrytis cinerea on different hosts
Journal of the Science of Food and Agriculture, 2019Co-Authors: Adria Carbo, Neus Teixidó, J Usall, R TorresAbstract:BACKGROUND The efficacy of Candida Sake CPA-1 as a biocontrol agent against several diseases has been studied since it was isolated 20 years ago. However, it was only recently that two suitable and effective film-forming formulations based on potato starch and maltodextrins were developed using the fluidized-bed spray-drying system. The present work aimed to confirm the capability of both novel formulations by testing their resilience on grapes at different temperatures (0, 22, and 30 °C), relative humidities (40% and 85%), and simulated rainfall levels. Another objective was to examine the control of Botrytis cinerea in different hosts. RESULTS The CPA-1 cells from both dried formulations survived better than the liquid formulation on grapes stored at 0 and 22 °C regardless of the relative humidity. After simulated rainfall, potato starch formulation achieved significantly higher populations than maltodextrin formulation, although the highest reduction was -1.6 log N N0-1 . A positive effect of cell establishment prior to the simulated rainfall was shown, and recovered cells from the potato starch formulation were significantly higher after 72 h of cell establishment. Finally, both formulations reduced the incidence and severity of B. cinerea on pears, apples, and tomatoes. CONCLUSION The potential of these novel film-forming formulations of C. Sake CPA-1 was verified. The resilience of formulated C. Sake was better than the commercialized liquid formulation, the adherence of the formulations to the grapes improved after an establishment period prior to rain exposure, and the control of B. cinerea was verified in a wider range of hosts. © 2019 Society of Chemical Industry.
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novel film forming formulations of the biocontrol agent Candida Sake cpa 1 biocontrol efficacy and performance at field conditions in organic wine grapes
Pest Management Science, 2019Co-Authors: Adria Carbo, Josep Usall, R Torres, A Marin, Amparo Chiralt, Neus TeixidóAbstract:This is the peer reviewed version of the following article: Carbo, A., Torres, R., Usall, J., Marin, A., Chiralt, A. and Teixido, N. (2019), Novel film?forming formulations of the biocontrol agent Candida Sake CPA?1: biocontrol efficacy and performance at field conditions in organic wine grapes. Pest. Manag. Sci., 75: 959-968, which has been published in final form at https://doi.org/10.1002/ps.5200. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
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Predicted ecological niches and environmental resilience of different formulations of the biocontrol yeast Candida Sake CPA-1 using the Bioscreen C
BioControl, 2018Co-Authors: Adria Carbo, R Torres, J Usall, N. Teixidó, N. Magan, Angel MedinaAbstract:Environmental resilience of biocontrol microorganisms has been a major bottleneck in the development of effective formulations. Candida Sake is an effective biocontrol agent (BCA) against Penicillium expansum, Botrytis cinerea or Rhizopus stolonifer , and different formulations of the BCA have been optimised recently. The objective of this study was to compare the relative tolerance of different dry and liquid formulations of the biocontrol yeast C. Sake CPA-1 to interacting environmental conditions using the Bioscreen C. Initially, the use of this automated turbidimetric method was optimised for use with different formulations of the biocontrol yeast. The best growth curves were obtained for the C. Sake CPA-1 strain when grown in a synthetic grape juice medium under continuous shaking and with an initial concentration of 10^5 CFUs ml^−1. All the formulations showed a direct relationship between optical density values and yeast concentrations. Temperature (15–30 °C) and water activity (a_w; 0.94–0.99) influenced the yeast resilience most profoundly, whereas the effect of pH (3–7) was minimal. In general, the liquid formulation grew faster in more interacting environmental conditions but only the yeast cells in the dry potato starch formulation could grow in some stress conditions. This rapid screening method can be used for effective identification of the resilience of different biocontrol formulations under interacting ecological abiotic conditions.
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impact of climate change environmental conditions on the resilience of different formulations of the biocontrol agent Candida Sake cpa 1 on grapes
Letters in Applied Microbiology, 2018Co-Authors: Adria Carbo, Josep Usall, Neus Teixidó, R Torres, Angel Medina, Naresh MaganAbstract:Biocontrol agents have become components of integrated crop protection systems for controlling economically important fungal pathogens. Candida Sake CPA-1 is a biocontrol agent of fungal pathogens of fruits, both pre- and post-harvest. While the efficacy of different formulations have been examined previously, few studies have considered the resilience of different formulations under changing climatic conditions of elevated temperature, drought stress and increased atmospheric CO2 . This study examined the effect of (a) temperature × RH × elevated CO2 (400 vs 1000 ppm) on the temporal establishment and viability of two dry and one liquid C. Sake CPA-1 formulations on grape berry surfaces; (b) temperature stress (25 vs 35°C); and (c) elevated CO2 levels. Results indicated that temperature, RH and CO2 concentration influenced the establishment and viability of the formulations but there was no significant difference between formulations. For the combined three-component factors, increased temperature (35°C) and lower RH (40%) reduced the viable populations on grapes. The interaction with elevated CO2 improved the establishment of viable populations of the formulations tested. Viable populations greater than Log 4 CFUs per g were recovered from the grape surfaces suggesting that these had conserved resilience for control of Botrytis rot in grapes. SIGNIFICANCE AND IMPACT OF THE STUDY The interaction between environmental factors that are expected to occur in response to climate change (CC) will have a significant impact on food security and availability. Little information exists on how elevated temperature, drought stress and increased CO2 will have on the efficacy of biocontrol agents. The impact of these factors on the viability of different formulations of the biocontrol yeast Candida Sake on the surface of grapes berries was evaluated for the first time. Such knowledge is critical for projecting the efficacy of biocontrol under climate change conditions and to identify formulations that have the necessary resilience to perform under CC conditions.
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dry formulations of the biocontrol agent Candida Sake cpa 1 using fluidised bed drying to control the main postharvest diseases on fruits
Journal of the Science of Food and Agriculture, 2017Co-Authors: Adria Carbo, R Torres, J Usall, Estanislau Fons, N. TeixidóAbstract:The biocontrol agent Candida Sake CPA-1 is effective against several diseases. Consequently, the optimisation of a dry formulation of C. Sake to improve its shelf life and manipulability is essential for increasing its potential with respect to future commercial applications. The present study aimed to optimise the conditions for making a dry formulation of C. Sake using a fluidised bed drying system and then to determine the shelf life of the optimised formulation and its efficacy against Penicillium expansum on apples.; Results: The optimal conditions for the drying process were found to be 40 °C for 45 min and the use of potato starch as the carrier significantly enhanced the viability. However, none of the protective compounds tested increased the viability of the dried cells. A temperature of 25 °C for 10 min in phosphate buffer was considered as the optimum condition to recover the dried formulations. The dried formulations should be stored at 4 °C and air-packaged; moreover, shelf life assays indicated good results after 12 months of storage. The formulated products maintained their biocontrol efficacy.; Conclusion: A fluidised bed drying system is a suitable process for dehydrating C. Sake cells; moreover, the C. Sake formulation is easy to pack, store and transport, and is a cost-effective process. © 2017 Society of Chemical Industry.; © 2017 Society of Chemical Industry.
Maribel Abadias - One of the best experts on this subject based on the ideXlab platform.
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fate of escherichia coli in apple and reduction of its growth using the postharvest biocontrol agent Candida Sake cpa 1
Journal of the Science of Food and Agriculture, 2009Co-Authors: Maribel Abadias, R Torres, J Usall, Isabel Alegre, Inmaculada VinasAbstract:BACKGROUND: Generally, acidic fruits and fruit juices are considered ‘safe’ from a microbiological point of view. However, some outbreaks of foodborne illnesses have been linked to the consumption of unpasteurised cider. The aim of this work was to study the survival of Escherichia coli in apple juice, wounds and flesh and on apple surfaces at different temperatures and to determine the effect of the fungal biocontrol agent Candida Sake CPA-1 against the colonisation of apple by E. coli. RESULTS: Trials were conducted with a mixture of five strains of E. coli isolated from apples. E. coli was unable to grow in apple juice at 5, 15 and 25 °C but survived. At 10 °C and above, E. coli thrived in fresh-cut apple and wounds. At 5 °C it survived in apple wounds after 27 days of storage and after 21 days in fresh-cut apples. When E. coli was inoculated in apple wounds together with the yeast antagonist C. Sake, its growth was reduced by approximately 1 log cfu wound−1 at 25 °C. At 5 °C no effect of the biocontrol agent was observed. CONCLUSION: Despite the low pH of apple, a rapid increase in the bacterial population is possible if the temperature is not kept low enough. The biocontrol agent C. Sake, developed to prevent fruit decay during storage, could also reduce E. coli growth in wounded apples at abusive temperatures. This would represent an additional benefit of using this biocontrol agent when applied to control postharvest diseases. Copyright © 2009 Society of Chemical Industry
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desarrollo produccion y formulacion comercial de la cepa cpa 1 de Candida Sake para el control de las principales enfermedades en postcosecha de fruta
Phytoma España: La revista profesional de sanidad vegetal, 2006Co-Authors: Inmaculada Vinas Almenar, Neus Teixidó, Maribel Abadias, Josep Usall Rodie, R TorresAbstract:El desarrollo de resistencias a los fungicidas por parte de muchos patogenos, conjuntamente con un creciente interes social sobre los riesgos medioambientales y para la salud que tienen estos pesticidas, han generado un gran interes en el desarrollo de metodos alternativos a los productos quimicos de sintesis. El control biologico de las enfermedades de poscosecha en fruta de pepita se ha mostrado como una de las alternativas mas prometedoras al control quimico. El esfuerzo que la investigacion dedica a esta area se ha incrementado de manera espectacular, y se refleja por la existencia de un elevado numero de patentes. Entre las patentes se encuentra la patente de la cepa CPA-1 de la levadura Candida Sake aislada de la superficie de manzanas y desarrollada en su totalidad por la unidad de patologia del area de postcosecha del Centro UdL-IRTA. Esta cepa ha demostrado tener gran actividad antagonica contra los principales patogenos de fruta de pepita y ya se han finalizado las etapas de produccion y formulacion, pasos claves que nos permiten optimizar la eficacia, seguridad , facilidad en la aplicacion y estabilidad con el fin de utilizarlos comercialmente como alternativa a los fungicidas quimicos.
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survival of the postharvest biocontrol yeast Candida Sake cpa 1 after dehydration by spray drying
Biocontrol Science and Technology, 2005Co-Authors: Maribel Abadias, Josep Usall, Neus Teixidó, C Solsona, I VinasAbstract:Abstract Spray drying was evaluated as a dehydrating method to preserve the postharvest biocontrol agent Candida Sake CPA-1. The effect of drying temperature, carrier, growth and rehydrating medium on the survival of the yeast was studied. Outlet temperature had more influence on the death of the cells than inlet temperature, and survival decreased with increasing temperature. Spray drying at an inlet temperature of 150°C was optimum in terms of viability, powder recovery and moisture content of the product. Use of 10% (v/v) skimmed milk as a carrier gave the highest survival and percentage of powder recovery (34–47%). Rich rehydration media were found to be better than water or phosphate buffer, with slight differences on survival. Spray-dried cells were less effective than fresh ones in controlling Penicillium expansum rot on apples. Spray drying of C. Sake was not a good dehydration method as it gave low cell survival, poor recovery of product, and low efficacy.
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optimization of growth conditions of the postharvest biocontrol agent Candida Sake cpa 1 in a lab scale fermenter
Journal of Applied Microbiology, 2003Co-Authors: Maribel Abadias, Josep Usall, Neus Teixidó, I VinasAbstract:Aim: To maximize the growth (expressed as number of viable cells per millilitre) of the postharvest biocontrol agent Candida Sake CPA-1 at laboratory scale conditions. Methods and Results: Growth conditons (aeration, agitation speed and inoculum size) were studied in batch conditions in a 5 l fermenter using molasses and urea as growth medium. Consumption of sugars and urea were analysed. Fed-batch studies were also carried out. Glucose and fructose were consumed during the exponential growth phase and were depleted after 18 h of growth. On the contrary, C. Sake cells assimilated sucrose during the stationary phase. There was not growth improvement when fed-batch technology was used. Addition of an extra amount of glucose or molasses after 18 h of growth did not contribute to increase final population. Conclusions: Maximum growth (about 8 × 108 CFU ml−1) was obtained at batch fermentation after 30 h growth at 400 rev min−1, 150 l h−1 of air and initial concentration of 106 CFU ml−1. Significance and Impact of the Study: The results obtained in this study are an approach for further upscaling of C. Sake production.
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Liquid Formulation of the Postharvest Biocontrol Agent Candida Sake CPA-1 in Isotonic Solutions.
Phytopathology, 2003Co-Authors: Maribel Abadias, Josep Usall, Neus Teixidó, Immaculada ViñasAbstract:ABSTRACT Viability of the postharvest biocontrol agent Candida Sake CPA-1 stored as liquid formulation was evaluated by studying the effect of growth, preservation medium, and temperature. C. Sake was grown in molasses medium with unmodified water activity (aw) and in the same with aw modified to 0.98 with the addition of several solutes. Cells were preserved with isotonic solutions of different substances. Efficacy of liquid formulations stored for different periods was tested against infection by Penicillium expansum on apples. The best growth media were the (unmodified one and those modified to 0.98 aw with the addition of glycerol or sorbitol. For all growth media, the best preservation medium was the isotonic solution prepared with trehalose. When the effect of trehalose concentration in the preservation medium was studied, generally, at trehalose concentrations below the isotonic one, C. Sake viabilities increased with increased trehalose. However, the best results were obtained when cells were pres...
