The Experts below are selected from a list of 1761 Experts worldwide ranked by ideXlab platform
Anuchai Niwetpathomwat - One of the best experts on this subject based on the ideXlab platform.
-
A complicated case of concurrent Canine babesiosis and Canine Ehrlichiosis
Comparative Haematology International, 2007Co-Authors: Sukullaya Assarasakorn, Anuchai NiwetpathomwatAbstract:Canine babesiosis and Canine Ehrlichiosis are the major tick-borne diseases throughout the world. The concurrent infection between Canine babesiosis and Canine Ehrlichiosis can occur in endemic regions. This report showed the clinical pathology and treatment outcome of concurrent infection in a dog admitted to a veterinary hospital. Based on the history, physical examination, and laboratory finding, the dog was diagnosed with neurological disorders induced by babesiosis and Ehrlichiosis concurrent infection. The dog presented with moderate normocytic normochromic anemia, severe thrombocytopenia and moderately increased alkaline phosphatase. The symptoms were more complicated with high fever, seizure, and cardiopulmonary arrest. The condition was specifically treated with doxycycline and diminazene aceturate, but the dog died 2 days after admission. In conclusion, concurrent infection of Canine babesiosis and Canine Ehrlichiosis can cause complicated clinical neurological compromise and result in mortality in dogs.
-
A retrospective study of the therapeutic efficacy of doxycycline on concurrent Canine Ehrlichiosis and babesiosis in a veterinary hospital population
Comparative Clinical Pathology, 2006Co-Authors: Anuchai NiwetpathomwatAbstract:Doxycycline has a well known broad spectrum activity against bacteria and rickettsia, as well as Ehrlichia spp. However, the use of doxycycline for the treatment of concurrent Ehrlichiosis and babesiosis has rarely been evaluated, especially in veterinary hospital populations. A retrospective study of 70 Canine Ehrlichiosis and 12 Canine babesiosis concurrent infections from Out Patient Department patients at Chulalongkorn Small Animal Teaching Hospital, admitted during 2001–2003, were studied. The results showed a complete curative effect of doxycycline on both Canine Ehrlichiosis and Canine babesiosis concurrent infections. The red blood cell indices after treatment were significantly higher in Canine Ehrlichiosis ( P
-
A retrospective study of the therapeutic efficacy of doxycycline on concurrent Canine Ehrlichiosis and babesiosis in a veterinary hospital population
Comparative Clinical Pathology, 2006Co-Authors: Anuchai NiwetpathomwatAbstract:Doxycycline has a well known broad spectrum activity against bacteria and rickettsia, as well as Ehrlichia spp. However, the use of doxycycline for the treatment of concurrent Ehrlichiosis and babesiosis has rarely been evaluated, especially in veterinary hospital populations. A retrospective study of 70 Canine Ehrlichiosis and 12 Canine babesiosis concurrent infections from Out Patient Department patients at Chulalongkorn Small Animal Teaching Hospital, admitted during 2001–2003, were studied. The results showed a complete curative effect of doxycycline on both Canine Ehrlichiosis and Canine babesiosis concurrent infections. The red blood cell indices after treatment were significantly higher in Canine Ehrlichiosis (P < 0.05). The platelet cell counts after treatment were significantly higher in concurrent Canine Ehrlichiosis and babesiosis infections (P < 0.05). Doxycycline can be recommended as the drug of choice for both Canine Ehrlichiosis and Canine babesiosis and concurrent infections of both conditions in veterinary hospitals.
-
A retrospective study of the clinical hematology and biochemistry of Canine Ehrlichiosis in an animal hospital population in Bangkok, Thailand
Comparative Clinical Pathology, 2005Co-Authors: Anuchai Niwetpathomwat, Somporn Techangamsuwan, Siram SuvarnavibhajaAbstract:A retrospective study on 687 Canine Ehrlichiosis cases was carried out during January 2001–December 2003 at the Chulalongkorn Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University. Data on rectal temperature, clinical hematology, and biochemistry were analyzed on the first day of admission. The highest incidences each year were observed in December 2001 (24 cases), June and July 2002 (31 cases each), and October 2003 (38 cases). The clinical findings varied, although most cases showed a high fever (33%), severe anemia (18%), leukocytosis (18%) or leukopenia (19%), thrombocytopenia (93%), and hepatic enzyme elevation. It followed that the therapeutic plan should comprise both specific and clinically supportive treatments, including hematotonics and hepatotonics.
Shimon Harrus - One of the best experts on this subject based on the ideXlab platform.
-
Guideline for veterinary practitioners on Canine Ehrlichiosis and anaplasmosis in Europe
Parasites & Vectors, 2015Co-Authors: Ángel Sainz, Shimon Harrus, Xavier Roura, Guadalupe Miró, Agustín Estrada-peña, Barbara Kohn, Laia Solano-gallegoAbstract:Canine Ehrlichiosis and anaplasmosis are important tick-borne diseases with a worldwide distribution. Information has been continuously collected on these infections in Europe, and publications have increased in recent years. Prevalence rates are high for Ehrlichia and Anaplasma spp. infections in dogs from different European countries. The goal of this article was to provide a practical guideline for veterinary practitioners on the diagnosis, treatment, and prevention of Ehrlichiosis and anaplasmosis in dogs from Europe. This guideline is intended to answer the most common questions on these diseases from a practical point of view.
-
Platelet dysfunction associated withexperimental acute Canine Ehrlichiosis
2013Co-Authors: Shimon Harrus, T Waner, A Eldor, E Zwang, Hylton BarkAbstract:the Practicinig Veterinarian 7, 569TROY,G. C. (1988) Veterinary Clinics ofNorthAmerica, SmallAnimnalPractice 18, 5WANER, T., HARRUS, S., WEISS, D. J., BARK, H. & KEYSARY, A. (1995)Veterinars ImmunologyandImmunopathology48, 177WOODY, B. J. & HOSKINS, J. D. (1991) Veterinary Clinics ofNorth America,SmnallAnimalPractice 21, 75ZUCKER, M. B. (1989) Methods in Enzymology. Vol 169. Platelets: Receptors,Adhesion, Secretion. EdJ. Hawiger. SanDiego, AcademicPress.
-
A serosurvey of Hepatozoon canis and Ehrlichia canis antibodies in wild red foxes (Vulpes vulpes) from Israel
Veterinary Parasitology, 2004Co-Authors: Zohar Fishman, Shimon Harrus, Liat Gonen, Dalit Strauss-ayali, Roni King, Gad BanethAbstract:A seroepidemiological survey was conducted to investigate the prevalence of antibodies reactive with Ehrlichia canis and Hepatozoon canis antigens in free-ranging red foxes (Vulpes vulpes) in Israel. Of 84 fox sera assayed, 36% were seropositive for E. canis by the indirect fluorescent antibody (IFA) test and 24% were positive for H. canis using an enzyme-linked immunosrbent assay (ELISA). Canine Ehrlichiosis and hepatozoonosis appear to be endemic in the wild red fox populations in Israel, and foxes may serve as a reservoir for infection of domestic dogs and other wild Canine species.
-
Characterization of the subclinical phase of Canine Ehrlichiosis in experimentally infected beagle dogs.
Veterinary Parasitology, 1997Co-Authors: T Waner, Shimon Harrus, Hylton Bark, E. Bogin, Y. Avidar, Avi KeysaryAbstract:Abstract Beagle dogs were examined during the subclinical phase of Canine Ehrlichiosis under controlled conditions. Emphasis was placed on gathering data before artificial inoculation with Ehrlichia canis , and comparing these data with those of the subclinical phase of the disease. In this study all dogs were clinically healthy throughout the 6 month examination period. All subclinically infected dogs had IFA antibody titers to E. canis at a dilution varying from 1:2560 to 1:20 480. The most prominent haematological finding was mild thrombocytopenia with a concomitant increase in platelet size, seen in eight of the nine dogs examined. Leukocyte counts were statistically significantly reduced in 78% of the dogs, compared with their preinfection values, with 71% of dogs having significantly reduced absolute neutrophil counts. None of the dogs were either leukopenic nor neutropenic. Six of the nine dogs had increased serum gamma-globulin concentrations. No dogs were overtly anemic, although declines in packed cell volume, haemoglobin concentration and total erythrocyte count were detected in an inconsistent manner among the dogs. It was concluded that, the most reliable parameters for judging possible subclinical ehrlichial infection in beagle dogs was mild thrombocytopenia, together with a persistently high antibody titer to E. canis . Hypergammaglobulinemia would increase the suspicion further. Based on the results presented, routine testing of dogs in E. canis endemic areas is recommended in order to identify and treat dogs in the subclinical phase of the disease.
-
platelet dysfunction associated with experimental acute Canine Ehrlichiosis
Veterinary Record, 1996Co-Authors: Shimon Harrus, T Waner, A Eldor, E Zwang, Hylton BarkAbstract:To determine whether platelet dysfunction occurs in Canine Ehrlichiosis, platelet aggregation studies in response to collagen/ epinephrine, thrombin and adenosine diphosphate (ADP) were carried out by the indirect method, using sera from six dogs experimentally infected with Ehrlichia canis . Samples of serum taken before infection and four and 20 days after infection were tested by incubation with platelet-rich plasma from a seronegative healthy dog. Platelet aggregation was significantly inhibited in five of six infected dogs in response to at least one of the agonists used. A significant increase in preaggregation lag time was recorded in response to collagen/ epinephrine in sera taken 20 days after infection from three of five dogs (P<0.05). When compared with the preinfection values, a significant increase of 45 per cent in the mean preaggregation lag time was detected (P<0.05). Maximal relative aggregation responses to ADP decreased significantly in one serum sample taken four days and one taken 20 days after infection (P<0.01) and there was a significantly lower relative slope for one serum sample 20 days after infection (P<0.05). Maximal relative aggregation responses to thrombin were significantly decreased together with their relative slopes in serum samples from two of four dogs four days after infection (P<0.05). The results suggest that platelet dysfunction may occur in the acute stage of Canine Ehrlichiosis, and may be a contributing factor to the tendency to bleed commonly observed in this disease. Antiplatelet antibodies directed against platelet glycoproteins may play a role in the inhibition of platelet aggregation.
Cristiane Divan Baldani - One of the best experts on this subject based on the ideXlab platform.
-
Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of Canine Ehrlichiosis
Revista Brasileira de Parasitologia Veterinária, 2010Co-Authors: Andréa Cristina Higa Nakaghi, Rosangela Zacarias Machado, Marcos Rogério André, Jesus Aparecido Ferro, Marcelo B. Labruna, Andreas Lazaros Chryssafidis, Cristiane Divan BaldaniAbstract:The aim of this study was to optimize a PCR assay that amplifies an 843 pb fragment from the p28 gene of Ehrlichia canis and compare it with two other PCR methods used to amplify portions of the 16S rRNA and dsb genes of Ehrlichia. Blood samples were collected from dogs suspected of having a positive diagnosis for Canine Ehrlichiosis. Amplification of the p28 gene by PCR produced an 843-bp fragment and this assay could detect DNA from one gene copy among 1 billion cells. All positive samples detected by the p28-based PCR were also positive by the 16S rRNA nested-PCR and also by the dsb-based PCR. Among the p28-based PCR negative samples, 55.3% were co-negatives, but 27.6% were positive in 16S rRNA and dsb based PCR assays. The p28-based PCR seems to be a useful test for the molecular detection of E. canis, however improvements in this PCR sensitivity are desired, so that it can become an important alternative in the diagnosis of Canine Ehrlichiosis.O objetivo deste estudo foi aperfeiçoar um ensaio de PCR que amplificasse um fragmento de 843 pares de bases do gene p28 da Ehrlichia canis e compará-lo com outros dois métodos de PCR utilizados para amplificar partes do gene 16S rRNA e dsb do gênero Ehrlichia. Amostras sanguíneas foram colhidas de cães com diagnóstico clínico de erliquiose. A amplificação do gene p28 pela PCR produziu um fragmento de 843pb e esse ensaio permitiu a detecção do DNA de um parasita dentre 1 bilhão de células. Todas as amostras positivas detectadas pela PCR baseada no gene p28 foram também positivas pela nested PCR para detecção do gene 16S rRNA e também pela PCR dsb. Dentre as amostras negativas para a PCR p28, 55,3% foram co-negativas, mas 27,6% foram positivas pela PCR baseada nos genes 16S rRNA e dsb. A PCR p28 parece ser um teste útil para detecção molecular de E. canis, entretanto otimizações na sensibilidade nesta PCR são necessárias, para que esta técnica se torne uma importante alternativa no diagnóstico da erliquiose canina
-
Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of Canine Ehrlichiosis
Revista Brasileira de Parasitologia Veterinária, 2010Co-Authors: Andréa Cristina Higa Nakaghi, Rosangela Zacarias Machado, Marcos Rogério André, Jesus Aparecido Ferro, Marcelo B. Labruna, Andreas Lazaros Chryssafidis, Cristiane Divan BaldaniAbstract:The aim of this study was to optimize a PCR assay that amplifies an 843 pb fragment from the p28 gene of Ehrlichia canis and compare it with two other PCR methods used to amplify portions of the 16S rRNA and dsb genes of Ehrlichia. Blood samples were collected from dogs suspected of having a positive diagnosis for Canine Ehrlichiosis. Amplification of the p28 gene by PCR produced an 843-bp fragment and this assay could detect DNA from one gene copy among 1 billion cells. All positive samples detected by the p28-based PCR were also positive by the 16S rRNA nested-PCR and also by the dsb-based PCR. Among the p28-based PCR negative samples, 55.3% were co-negatives, but 27.6% were positive in 16S rRNA and dsb based PCR assays. The p28-based PCR seems to be a useful test for the molecular detection of E. canis, however improvements in this PCR sensitivity are desired, so that it can become an important alternative in the diagnosis of Canine Ehrlichiosis.
-
Canine Ehrlichiosis: clinical, hematological, serological and molecular aspects
Ciência Rural, 2008Co-Authors: Andréa Cristina Higa Nakaghi, Rosangela Zacarias Machado, Mirela Tinucci Costa, Marcos Rogério André, Cristiane Divan BaldaniAbstract:The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques it was concluded that serology and nPCR are the most suitable tests to confirm the diagnosis of Canine Ehrlichiosis, however it should be always treated as a complementary data to clinical and hematological evaluation. Serology has an important role in the subclinical and in the chronic phase, nPCR is recommended in the acute stage, and, especially, to identify the ehrlichia specie.
Rosangela Zacarias Machado - One of the best experts on this subject based on the ideXlab platform.
-
Influence of experimental Canine Ehrlichiosis on the E-ADA activity and purine levels in serum and possible functional correlations with pathogenesis
Veterinary Microbiology, 2013Co-Authors: Aleksandro S. Da Silva, Rosangela Zacarias Machado, Nathieli B. Bottari, Thiago Demarchi Munhoz, Joice Lara Maia Faria, Giovanni Vargas-hernandez, Nathalia Carvalho Da Luz, Cesar Eduardo Jacintho Moritz, Emerson André Casali, Lenita M. StefaniAbstract:The aim of this study was to evaluate adenosine deaminase activity and purines levels in serum of dogs experimentally infected by Ehrlichia canis. Banked serum samples of dogs divided into two groups with five animals each: healthy animals and animals infected by E. canis. The concentration of purines (adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP), adenosine, inosine, hypoxanthine, xanthine and uric acid), and adenosine deaminase (E-ADA) activity in sera were evaluated. Samples were collected on days 12 and 30 post-infection (PI). The E-ADA activity showed a significant reduction on day 12 PI, and increased on day 30 PI in dogs infected with E. canis. On day 12, an increase in seric concentration of ATP, ADP and adenosine was verified, and different levels of hypoxanthine, xanthine and uric acid had a drastic reduction in infected compared healthy dogs (P < 0.05). However, on day 30 PI, the levels of seric ADP and AMP decreased, unlike the concentration of xanthine and uric acid that increased significantly in infected dogs (P < 0.05). Therefore, the activity of E-ADA and purine levels are altered in experimental Canine Ehrlichiosis, probably with the purpose of modulating the pathogenesis of the disease related to immune response, oxidative stress and coagulation disorders in acute phase.
-
Experimental Ehrlichia canis infection changes acute-phase proteins.
Revista Brasileira de Parasitologia Veterinária, 2012Co-Authors: Thiago Demarchi Munhoz, Rosangela Zacarias Machado, Joice Lara Maia Faria, Giovanni Vargas-hernandez, José Jurandir Fagliari, Aureo Evangelista Santana, Mirela Tinucci-costaAbstract:Early diagnosis of Canine Ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of Canine Ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.
-
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-α in leukocytes and splenocytes of experimentally infected dogs
Revista Brasileira de Parasitologia Veterinária, 2011Co-Authors: Joice Lara Maia Faria, Rosangela Zacarias Machado, Marcos Rogério André, Thiago Demarchi Munhoz, C. F. João, Giovanny Vargas-hernández, Wanderson Adriano Biscola Pereira, Mirela Tinucci-costaAbstract:Canine Ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-α, IL-10, IFN-γ, in splenic cells and blood leukocytes during the acute phase of Ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-α 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-γ 18 and 30 days post-inoculation, respectively. This study suggests that TNF-α plays a role in the pathogenesis of the acute phase of Canine Ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia.
-
Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of Canine Ehrlichiosis
Revista Brasileira de Parasitologia Veterinária, 2010Co-Authors: Andréa Cristina Higa Nakaghi, Rosangela Zacarias Machado, Marcos Rogério André, Jesus Aparecido Ferro, Marcelo B. Labruna, Andreas Lazaros Chryssafidis, Cristiane Divan BaldaniAbstract:The aim of this study was to optimize a PCR assay that amplifies an 843 pb fragment from the p28 gene of Ehrlichia canis and compare it with two other PCR methods used to amplify portions of the 16S rRNA and dsb genes of Ehrlichia. Blood samples were collected from dogs suspected of having a positive diagnosis for Canine Ehrlichiosis. Amplification of the p28 gene by PCR produced an 843-bp fragment and this assay could detect DNA from one gene copy among 1 billion cells. All positive samples detected by the p28-based PCR were also positive by the 16S rRNA nested-PCR and also by the dsb-based PCR. Among the p28-based PCR negative samples, 55.3% were co-negatives, but 27.6% were positive in 16S rRNA and dsb based PCR assays. The p28-based PCR seems to be a useful test for the molecular detection of E. canis, however improvements in this PCR sensitivity are desired, so that it can become an important alternative in the diagnosis of Canine Ehrlichiosis.
-
Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of Canine Ehrlichiosis
Revista Brasileira de Parasitologia Veterinária, 2010Co-Authors: Andréa Cristina Higa Nakaghi, Rosangela Zacarias Machado, Marcos Rogério André, Jesus Aparecido Ferro, Marcelo B. Labruna, Andreas Lazaros Chryssafidis, Cristiane Divan BaldaniAbstract:The aim of this study was to optimize a PCR assay that amplifies an 843 pb fragment from the p28 gene of Ehrlichia canis and compare it with two other PCR methods used to amplify portions of the 16S rRNA and dsb genes of Ehrlichia. Blood samples were collected from dogs suspected of having a positive diagnosis for Canine Ehrlichiosis. Amplification of the p28 gene by PCR produced an 843-bp fragment and this assay could detect DNA from one gene copy among 1 billion cells. All positive samples detected by the p28-based PCR were also positive by the 16S rRNA nested-PCR and also by the dsb-based PCR. Among the p28-based PCR negative samples, 55.3% were co-negatives, but 27.6% were positive in 16S rRNA and dsb based PCR assays. The p28-based PCR seems to be a useful test for the molecular detection of E. canis, however improvements in this PCR sensitivity are desired, so that it can become an important alternative in the diagnosis of Canine Ehrlichiosis.O objetivo deste estudo foi aperfeiçoar um ensaio de PCR que amplificasse um fragmento de 843 pares de bases do gene p28 da Ehrlichia canis e compará-lo com outros dois métodos de PCR utilizados para amplificar partes do gene 16S rRNA e dsb do gênero Ehrlichia. Amostras sanguíneas foram colhidas de cães com diagnóstico clínico de erliquiose. A amplificação do gene p28 pela PCR produziu um fragmento de 843pb e esse ensaio permitiu a detecção do DNA de um parasita dentre 1 bilhão de células. Todas as amostras positivas detectadas pela PCR baseada no gene p28 foram também positivas pela nested PCR para detecção do gene 16S rRNA e também pela PCR dsb. Dentre as amostras negativas para a PCR p28, 55,3% foram co-negativas, mas 27,6% foram positivas pela PCR baseada nos genes 16S rRNA e dsb. A PCR p28 parece ser um teste útil para detecção molecular de E. canis, entretanto otimizações na sensibilidade nesta PCR são necessárias, para que esta técnica se torne uma importante alternativa no diagnóstico da erliquiose canina
Laia Solano-gallego - One of the best experts on this subject based on the ideXlab platform.
-
Guideline for veterinary practitioners on Canine Ehrlichiosis and anaplasmosis in Europe
Parasites & Vectors, 2015Co-Authors: Ángel Sainz, Shimon Harrus, Xavier Roura, Guadalupe Miró, Agustín Estrada-peña, Barbara Kohn, Laia Solano-gallegoAbstract:Canine Ehrlichiosis and anaplasmosis are important tick-borne diseases with a worldwide distribution. Information has been continuously collected on these infections in Europe, and publications have increased in recent years. Prevalence rates are high for Ehrlichia and Anaplasma spp. infections in dogs from different European countries. The goal of this article was to provide a practical guideline for veterinary practitioners on the diagnosis, treatment, and prevention of Ehrlichiosis and anaplasmosis in dogs from Europe. This guideline is intended to answer the most common questions on these diseases from a practical point of view.
