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Miriam Dupas Hubinger - One of the best experts on this subject based on the ideXlab platform.

  • Quantifying the Responses of Three Bacillus cereus Strains in Isothermal Conditions and During Spray Drying of Different Carrier Agents.
    Frontiers in Microbiology, 2018
    Co-Authors: Verônica O. Alvarenga, Miriam Dupas Hubinger, Fernanda B. Campagnollo, Deborah A. Conceição, Yuri Abud, Celso Sant’anna, Anderson S. Sant'ana
    Abstract:

    Spray drying is a widely used method for producing milk powder. This process is not aimed to cause microbial inactivation, thus sporeforming bacteria may be abundant in the microbiota of milk powder. The first aim of this study was to determine the inactivation kinetics parameters in capillary tubes of three Bacillus cereus strains (436, B63, 540) in three menstrua (whole milk, phosphate buffer and talc suspension) at 90, 100 and 110oC. D-values for B. cereus in the three menstrua were not significantly different at the highest tested temperature (p>0.05). Thus, talc was chosen as a Carrier Agent to allow the recovery of B. cereus from spray dried materials given its low interference on inactivation kinetics. B. cereus spores were also inoculated in whole milk and skim milk following spray drying at 95°C, 105°C and 110°C (outlet temperature). After the spray drying runs, B. cereus spores were counted and the number of decimal reductions (γ) calculated. A correlation between the small diameter of the particles with the survival of spores of three B. cereus strains was found, and B. cereus 436 presented consistently the lowest γ no matter temperature and a Carrier Agent. The highest γ was found when talc powder was used, which suggest that this Carrier Agent does not protect B. cereus spores during spray drying. Spray drying of milk can lead to up to 4 γ (strain 540) of B. cereus spores but depending on the strain less than one γ (strain 436) could be observed. This study contributes to the knowledge on the microbiology of low water activity foods by providing novel findings regarding the fate of three B. cereus strains to different spray drying conditions. Acknowledging the variability of inactivation of B. cereus during spray drying is key in the current context of food safety in which the quantification of effects of unit operations must be known for the validation of processes and development of more robust formulations.

  • Volatile composition and physicochemical characteristics of mussel (Perna perna) protein hydrolysate microencapsulated with maltodextrin and n-OSA modified starch
    Food and Bioproducts Processing, 2017
    Co-Authors: Nirse Ruscheinsky Breternitz, Vanessa Martins Da Silva, Carlos Henrique De Vasconcelos Fidelis, Marcos N. Eberlin, Miriam Dupas Hubinger
    Abstract:

    Abstract The aim of this work was to study the effect of the process conditions on the microencapsulation of mussel (Perna perna) protein hydrolysate, particularly with respect to volatile retention. Thus, the influence of the inlet/outlet drying air temperatures (180/80 °C and 210/100 °C) over the powder volatile composition and physicochemical characteristics was evaluated. Simultaneously, the concentrations of the Carrier Agents, composed by proportions of maltodextrin 10DE (MD) and modified starch HiCap®100 (HC) (0:100, 50:50, 75:25 and 100:0, respectively) in three different additions (3.7, 10.2 and 16.0%) was varied. Increasing the Carrier Agent concentration resulted in an increase in the microparticles diameter and reduction in powder hygroscopicity and Tg values. HC resulted in more breakable microparticles, while MD provided the formation of rougher microparticles with a greater variation in size. Glass transition temperature and concentration of volatile compounds increased with higher drying temperatures and by the combination and concentration (the higher the better) of the Carrier Agents. Mussel hydrolysate powder spray dried at 210 °C as inlet drying temperature, with 16% of combined MD/HC (ratio 50/50) as Carrier Agent, showed a retention of hexanal, heptanal and octanal, respectively, 9.0, 4.6 and 4.2 times higher than in the pure hydrolysate powder. Therefore, this sample resulted in a good volatile concentration and physicochemical characteristics that can promote powder stability and was chosen as the best microencapsulation condition.

  • Influence of Carrier Agents on the Physicochemical Properties of Mussel Protein Hydrolysate Powder
    Drying Technology, 2012
    Co-Authors: Vanessa Martins Da Silva, Louise Emy Kurozawa, K. J. Park, Miriam Dupas Hubinger
    Abstract:

    The objective of this work was to evaluate the influence of Carrier Agents, maltodextrin 10 dextrose equivalent (DE) and gum arabic, on the physicochemical properties of mussel meat protein hydrolysate powder produced by spray drying. Hydrolysate was obtained by enzymatic hydrolysis using Protamex (Novozymes, Bagsvaerd, Denmark) and was carried out at 51°C, 4.5 g enzyme/100 g protein, and pH 6.85. The hydrolysate, without and with 15 and 30% of Carrier Agent, was spray dried at 180°C inlet air temperature and 0.8 L/h feed flow rate. Moisture content, hygroscopicity, particle size, glass transition temperature, morphology, antioxidant capacity, and volatile loss of powders were evaluated. Powder moisture content decreased with the increase in Carrier Agent concentration. Glass transition temperature increased with Carrier Agent addition and consequently powder hygroscopicity was reduced, increasing its physical stability. Higher feed solution viscosity was obtained for 30% of maltodextrin and 15 and 30% of...

  • Water Sorption and Glass Transition Temperature of Spray-Dried Mussel Meat Protein Hydrolysate
    Drying Technology, 2011
    Co-Authors: Vanessa Martins Da Silva, Louise Emy Kurozawa, K. J. Park, Miriam Dupas Hubinger
    Abstract:

    The water sorption behavior at 25°C and glass transition temperature (T g ) of mussel meat protein hydrolysate powder without and with maltodextrin 10 DE or gum arabic at 15 and 30% (w/w) were studied in this work. The sorption isotherms were determined by the gravimetric method, and the glass transition temperature was obtained by differential scanning calorimetry (DSC) after powder conditioning at various water activities. Sorption isotherms data were well fitted by a modified Brunauer-Emmett-Teller (BET) model. Powder without additives showed the highest water adsorption followed by those produced with 15 and 30% of Carrier Agent, respectively. The Gordon-Taylor model was able to predict the effect of water on the glass transition temperature. At 25°C, the critical water content that ensured the glassy state of the mussel hydrolysate powder during storage increased from 0.05 to 0.12 g water/g product and the critical water activity increased from 0.24 to 0.60 when the concentration of Carrier Agents wa...

  • spray drying of acai euterpe oleraceae mart juice effect of inlet air temperature and type of Carrier Agent
    Journal of Food Processing and Preservation, 2011
    Co-Authors: Renata V Tonon, Samuel Scarassatti Freitas, Miriam Dupas Hubinger
    Abstract:

    Physical and morphological characteristics of powdered acai juice were evaluated in this work. Powders produced by spray drying at different temperatures (140, 170 and 200C) using different Carrier Agents (maltodextrin 10DE, maltodextrin 20DE and gum Arabic) were evaluated for moisture content, water activity, hygroscopicity, bulk density, particle size distribution and morphology. Sorption isotherms were also constructed. The samples produced with maltodextrin 10DE showed the highest moisture content, water activity and mean diameter and were the less hygroscopic. The increase in drying temperature resulted in particles with lower moisture content, water activity and bulk density. Moreover, powders produced at higher temperatures were more hygroscopic and exhibited a greater number of particles with smooth surface. Sorption isotherms were of type III and showed a better fit to Guggenheim-Anderson-de Boe and Brunauer-Emmett-Teller models. PRACTICAL APPLICATIONS The present study provides useful information about the production of powdered acai juice by spray drying. In this process, the addition of a Carrier Agent is normally necessary in order to avoid problems like stickiness on the dryer wall, which would be negative to process yield and product quality. Moreover, among the process variables related to spray drying, the inlet air temperature can be considered very important, since it is directly related with powder quality, as well as with energy saving/wasting. Thus, in this work, the effect of three different types of Carrier Agents and three inlet air temperatures on the physicochemical properties of spray-dried acai juice was evaluated. The results can be useful for food and cosmetic companies looking for novel and inexpensive sources of natural pigments, to be used as colorants and flavorings.

Azlan Kamari - One of the best experts on this subject based on the ideXlab platform.

  • N-hexanoyl-O-glycol chitosan as a Carrier Agent for water-insoluble herbicide
    Journal of Physics: Conference Series, 2018
    Co-Authors: S N M Yusoff, Azlan Kamari, S Ishak, A. L. A. Halim
    Abstract:

    The objective of this study was to assess the potential of an amphiphilic chitosan derivative, namely N-hexanoyl-O-glycol chitosan (HGC) as a Carrier Agent for herbicide atrazine. The physicochemical properties of HGC were characterised using several analytical instruments. The critical micelle concentration (CMC) of HGC was determined using a Fluorescence Spectrometer. A High Performance Liquid Chromatography (HPLC) was used to determine encapsulation efficiency and loading capacity of atrazine in the amphiphilic chitosan derivative. Research findings found that the addition of hexanoyl and glycol groups to the chitosan backbone has improved the thermal stability of chitosan. TEM observation confirmed that HGC can form self-aggregation in the solution with spherical shape. The CMC values determined for HGC was 0.008 mg/mL. HGC also exhibited excellent encapsulation efficiency and loading capacity. The release profiles of atrazine loaded in HGC showed that it has different release behaviour than the pure herbicide in solution. In conclusion, the results from the study suggest that the amphiphilic chitosan derivative is applicable to be utilised as Carrier Agent for herbicide atrazine in pesticide formulation.

  • Synthesis, characterisation and potential application of deoxycholic acid carboxymethyl chitosan as a Carrier Agent for rotenone
    Journal of Polymer Research, 2018
    Co-Authors: N. F. A. Aljafree, Azlan Kamari
    Abstract:

    In the present study, deoxycholic acid carboxymethyl chitosan (DACMC) was synthesised via a two-step reaction, namely carboxymethylation and alkylation. Fourier Transform Infrared (FTIR) Spectrometer, Proton Nuclear Magnetic Resonance (^1H NMR) Spectrometer, Transmission Electron Microscope (TEM) and Thermogravimetric Analyser (TGA) were used to characterise DACMC. Spherical self-aggregates of DACMC micelles with the size ranging from 91.3 to 140.0 nm was observed. DACMC was soluble in pH range studied (1–13), except pH 4. DACMC micelles were formed at critical concentration (CMC) value of 0.468 mg/mL. The ability of DACMC to encapsulate and load rotenone was determined at different weight ratios. The highest value of encapsulation efficiency (EE%) (more than 98%) was obtained for weight ratio of 100:1 (DACMC:Rotenone). The in vitro release data of rotenone-loaded DACMC followed Ritger and Peppas Case II transport mechanism. Results from this study highlight the potential of DACMC to reduce organic solvent application in water-insoluble pesticide production.

  • N,N-dimethylhexadecyl carboxymethyl chitosan as a potential Carrier Agent for rotenone.
    International Journal of Biological Macromolecules, 2016
    Co-Authors: Azlan Kamari, N. F. A. Aljafree, S N M Yusoff
    Abstract:

    Abstract In this study, an amphiphilic chitosan derivative namely N,N -dimethylhexadecyl carboxymethyl chitosan (DCMC) was synthesised and applied for the first time as a Carrier Agent for rotenone. The physical and chemical properties of DCMC were characterised by using Fourier Transform Infrared Spectrometer (FTIR), Proton Nuclear Magnetic Resonance Spectrometer ( 1 H NMR), CHN-O Elemental Analyser, Thermogravimetric Analyser (TGA) and Differential Scanning Calorimeter (DSC). DCMC was soluble in acidic (except pH 4), neutral and basic media with percent of transmittance (%T) values ranged from 67.2 to 99.4%. The critical micelle concentration (CMC) was determined as 0.095 mg/mL. Transmission Electron Microscopy (TEM) analysis confirmed that DCMC has formed self-aggregates and exhibited spherical shape with the size of 65.5–137.0 nm. The encapsulation efficiency (EE) and loading capacity (LC) of DCMC micelles with different weight ratios (DCMC:rotenone; 5:1, 50:1 and 100:1) were determined by using High Performance Liquid Chromatography (HPLC). The weight ratio of 100:1 gave the best EE with the value of more than 95.0%. DCMC micelles performed an excellent ability to control the release of rotenone, of which 99.0% of rotenone was released within 48 h. Overall, DCMC has several key features to be an effective Carrier Agent for pesticide formulations.

  • Oleoyl-carboxymethyl chitosan as a new Carrier Agent for the rotenone pesticide
    Environmental Chemistry Letters, 2016
    Co-Authors: Azlan Kamari, N. F. A. Aljafree, S N M Yusoff
    Abstract:

    Rotenone is an active ingredient that has been widely used as pesticide. However, rotenone is poorly soluble in water. Therefore, a water-soluble Carrier is needed. Here, we synthesised, characterised and applied oleoyl-carboxymethyl chitosan for the first time as a Carrier Agent for rotenone. Oleoyl-carboxymethyl chitosan is soluble in acidic, neutral and basic media. The critical micelle concentration of oleoyl-carboxymethyl chitosan is 0.096 mg/mL. Oleoyl-carboxymethyl chitosan formed self-aggregates and exhibited spherical shape with the values of 35.5–66.4 nm. We studied the encapsulation efficiency and loading capacity of oleoyl-carboxymethyl chitosan micelles with different weight ratios of oleoyl-carboxymethyl chitosan to rotenone. We found an encapsulation efficiency of 97 % and a loading capacity of 0.97 % for oleoyl-carboxymethyl chitosan/rotenone 100:1 w/w. Oleoyl-carboxymethyl chitosan released 96.00 % of the rotenone loaded within 50 h. Overall, our findings show the feasibility of oleoyl-carboxymethyl chitosan as a novel water-solubilising Agent for pesticide formulations.

Samuel R Money - One of the best experts on this subject based on the ideXlab platform.

  • orally administered unfractionated heparin with Carrier Agent is therapeutic for deep venous thrombosis
    Circulation, 2000
    Co-Authors: Mark D Gonze, Khashayar Salartash, Charles W Sternbergh, Robert A Baughman, Andrea Leonebay, Samuel R Money
    Abstract:

    Background—Orally administered heparin (OHEP) is unreliable because of poor absorption. Sodium N-(8[2-hydroxybenzoyl]amino) caprylate (SNAC) is an amido acid that facilitates the gastrointestinal absorption of heparin. We evaluated the effectiveness of OHEP combined with SNAC (OHEP/SNAC) in the treatment of deep-vein thrombosis (DVT). Methods and Results—An internal jugular DVT was produced in 54 male Sprague-Dawley rats. Animals were assigned to 6 different groups for 7 days of treatment: untreated control, subcutaneous heparin (SC HEP) (300 U/kg SC TID), SNAC only (300 mg/kg PO TID), OHEP only (30 mg/kg PO TID), low-molecular-weight heparin (LMWH) (enoxaparin 5 mg/kg SC QD), and OHEP/SNAC (30 mg/kg:300 mg/kg PO TID). The activated partial thromboplastin time (aPTT) and anti–factor X (anti-Xa) levels were measured. The incidence of residual DVT after 1 week of treatment was 100% (9 of 9) in the control group versus 10% (1 of 10) in the OHEP/SNAC and 10% (1 of 10) in the LMWH groups (P<0.001). There was a...

  • Orally Administered Unfractionated Heparin With Carrier Agent Is Therapeutic for Deep Venous Thrombosis
    Circulation, 2000
    Co-Authors: Mark D Gonze, Khashayar Salartash, Robert A Baughman, W. Charles Sternbergh, Andrea Leone-bay, Samuel R Money
    Abstract:

    Background—Orally administered heparin (OHEP) is unreliable because of poor absorption. Sodium N-(8[2-hydroxybenzoyl]amino) caprylate (SNAC) is an amido acid that facilitates the gastrointestinal absorption of heparin. We evaluated the effectiveness of OHEP combined with SNAC (OHEP/SNAC) in the treatment of deep-vein thrombosis (DVT). Methods and Results—An internal jugular DVT was produced in 54 male Sprague-Dawley rats. Animals were assigned to 6 different groups for 7 days of treatment: untreated control, subcutaneous heparin (SC HEP) (300 U/kg SC TID), SNAC only (300 mg/kg PO TID), OHEP only (30 mg/kg PO TID), low-molecular-weight heparin (LMWH) (enoxaparin 5 mg/kg SC QD), and OHEP/SNAC (30 mg/kg:300 mg/kg PO TID). The activated partial thromboplastin time (aPTT) and anti–factor X (anti-Xa) levels were measured. The incidence of residual DVT after 1 week of treatment was 100% (9 of 9) in the control group versus 10% (1 of 10) in the OHEP/SNAC and 10% (1 of 10) in the LMWH groups (P

Renata V Tonon - One of the best experts on this subject based on the ideXlab platform.

  • Estabilidade da polpa de morango atomizada utilizando diferentes Agentes carreadores Stability of spray-dryed strawberry pulp produced with different Carrier Agents
    2020
    Co-Authors: Maria Isabel, Renata V Tonon, R. I. Nogueira, Lourdes Maria Corrêa Cabral
    Abstract:

    Summary This study aimed to evaluate the effect of different Carrier Agents (5DE Maltodextrin, 10DE Maltodextrin, gum Arabic and Capsul ® ) on the physicochemical characteristics (moisture content, density, solubility, total anthocyanins and antioxidant activity) of strawberry pulp processed by spray drying and stored at room temperature for 90 days. The use of different Carrier Agents resulted in powders with bulk densities ranging from 0.40 to 0.52 g/cm 3 and moisture contents below 2.5%. All the samples were highly soluble, but the powders obtained with 5DE Maltodextrin and gum Arabic were less hygroscopic. The sorption isotherms were of type III and the GAB model was the model that best fitted the experimental data. The powder produced with gum Arabic had the highest anthocyanin content and consequently the greatest antioxidant activity value, both immediately after the drying process and during the storage period. The results obtained showed that gum Arabic was the most suitable Carrier Agent for the production of strawberry pulp powder.

  • Spray drying of juçara pulp aiming to obtain a “pure” powdered pulp without using Carrier Agents
    Drying Technology, 2019
    Co-Authors: Danielle C. De S. Pereira, Renata V Tonon, Carolina Beres, Flávia Dos Santos Gomes, Lourdes M.c. Cabral
    Abstract:

    The aim of this work was to investigate the possibility of obtaining a jucara pulp powder by spray drying with no Carrier Agent addition. The pulp was filtered and then dried in a lab scale spray d...

  • spray drying of acai euterpe oleraceae mart juice effect of inlet air temperature and type of Carrier Agent
    Journal of Food Processing and Preservation, 2011
    Co-Authors: Renata V Tonon, Samuel Scarassatti Freitas, Miriam Dupas Hubinger
    Abstract:

    Physical and morphological characteristics of powdered acai juice were evaluated in this work. Powders produced by spray drying at different temperatures (140, 170 and 200C) using different Carrier Agents (maltodextrin 10DE, maltodextrin 20DE and gum Arabic) were evaluated for moisture content, water activity, hygroscopicity, bulk density, particle size distribution and morphology. Sorption isotherms were also constructed. The samples produced with maltodextrin 10DE showed the highest moisture content, water activity and mean diameter and were the less hygroscopic. The increase in drying temperature resulted in particles with lower moisture content, water activity and bulk density. Moreover, powders produced at higher temperatures were more hygroscopic and exhibited a greater number of particles with smooth surface. Sorption isotherms were of type III and showed a better fit to Guggenheim-Anderson-de Boe and Brunauer-Emmett-Teller models. PRACTICAL APPLICATIONS The present study provides useful information about the production of powdered acai juice by spray drying. In this process, the addition of a Carrier Agent is normally necessary in order to avoid problems like stickiness on the dryer wall, which would be negative to process yield and product quality. Moreover, among the process variables related to spray drying, the inlet air temperature can be considered very important, since it is directly related with powder quality, as well as with energy saving/wasting. Thus, in this work, the effect of three different types of Carrier Agents and three inlet air temperatures on the physicochemical properties of spray-dried acai juice was evaluated. The results can be useful for food and cosmetic companies looking for novel and inexpensive sources of natural pigments, to be used as colorants and flavorings.

  • anthocyanin stability and antioxidant activity of spray dried acai euterpe oleracea mart juice produced with different Carrier Agents
    Food Research International, 2010
    Co-Authors: Renata V Tonon, Catherine Brabet, Miriam Dupas Hubinger
    Abstract:

    Anthocyanin stability and antioxidant activity of powdered acai juice was evaluated throughout 120 days. Powders were produced by spray drying using four types of Carrier Agents: maltodextrin 10DE, maltodextrin 20DE, gum Arabic and tapioca starch. Samples were stored at different temperatures (25 and 35 °C) and water activities (0.328 and 0.529), in order to verify the effect of these conditions on anthocyanin degradation and antioxidant activity reduction. Anthocyanin degradation exhibited two first-order kinetics: the first one, with higher reaction rate constant, up to 45–60 days of storage, and the second one, after this period, with lower degradation rate. Both temperature and water activity negatively affected anthocyanin stability. Antioxidant activity also decreased with increasing water activity, but was higher for the powders stored at 35 °C. Maltodextrin 10DE was the Carrier Agent that showed the best pigment protection and the highest antioxidant activity, for all the conditions studied.

  • influence of process conditions on the physicochemical properties of acai euterpe oleraceae mart powder produced by spray drying
    Journal of Food Engineering, 2008
    Co-Authors: Renata V Tonon, Catherine Brabet, Miriam Dupas Hubinger
    Abstract:

    Abstract The objective of this work was to study the influence of spray drying conditions on the physicochemical properties of acai powder. The process was carried out on a mini spray dryer and maltodextrin 10DE was used as Carrier Agent. Seventeen tests were made, according to a central composite design. Independent variables were: inlet air temperature (138–202 °C), feed flow rate (5–25 g/min) and maltodextrin concentration (10–30%). Moisture content, hygroscopicity, process yield and anthocyanin retention were analysed as responses. Powder moisture content and process yield were positively affected by inlet air temperature and negatively affected by feed flow rate, which are directly related to heat and mass transfer. Process yield was also negatively influenced by maltodextrin concentration, due to the increase on mixture viscosity. Powders hygroscopicity decreased with increasing maltodextrin concentration, decreasing temperature and increasing feed flow rate. Powders with lower moisture content were more hygroscopic, which is related to the greater water concentration gradient between the product and the surrounding air. Anthocyanin retention was only affected by temperature, due to its high sensitivity. In respect to morphology, the particles produced at higher temperature were larger and a great number of them showed smooth surface.

Matheus B. Braga - One of the best experts on this subject based on the ideXlab platform.

  • Spray-drying of grape skin-whey protein concentrate mixture
    Journal of Food Science and Technology, 2018
    Co-Authors: Beatriz E. Oliveira, Paulo C.g. Junior, Lilian P. Cilli, Luana R. F. Contini, Anna C. Venturini, Cristiana M. P. Yoshida, Matheus B. Braga
    Abstract:

    The aim of this work was to evaluate the effect of process conditions (inlet air temperature, paste flow rate, and whey protein concentration) on the physical and physicochemical characteristics of the powder obtained from spray dried grape skin-whey protein concentrate mixture. The experiments were performed according to the 2^3 factorial design, with repetition of the central point. The highest values of anthocyanin retention (> 92%) were obtained with the highest inlet air temperature (190 °C) and the highest whey protein concentration (15% (w/w)). The powder also showed solubility above 78.3%, and low values of moisture content, hygroscopicity and bulk density. Spray drying of grape residues, using whey protein as Carrier Agent, resulted in a powder with a high level of anthocyanin retention and of good quality, showing a potential application in food products. Therefore, the method indicates a possible alternative to change post-harvesting waste in a natural source of bioactive compounds.