The Experts below are selected from a list of 108564 Experts worldwide ranked by ideXlab platform
Luke P Lee - One of the best experts on this subject based on the ideXlab platform.
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single Cell enzyme concentrations kinetics and inhibition analysis using high density hydrodynamic Cell Isolation arrays
Analytical Chemistry, 2006Co-Authors: Dino Di Carlo, Nima Aghdam, Luke P LeeAbstract:High-quality single-Cell data are required for a quantitative systems biology description of Cellular function. However, data of this type are difficult and time-consuming to collect using traditional techniques. We present a robust and simple microfluidic method for trapping single Cells in large arrays to address this problem. Ordered single-Cell Isolation arrays allow for high-density microscopic analysis with simplified image processing. Moreover, for fluorescent assays, on-chip sample preparation (e.g., fluorescent labeling, washing) can be performed, as opposed to manual intensive operations of incubation, centrifugation, and resuspension in previous techniquessaving time and reagents. This technology was applied to determine novel single-Cell enzyme kinetics for three different Cell types (HeLa, 293T, Jurkat). A kinetic model of this process predicted this varied response was due to variation in the concentration of carboxylesterase between Cell types. Nordihydroguaiaretic acid (NDGA) was also char...
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single Cell enzyme concentrations kinetics and inhibition analysis using high density hydrodynamic Cell Isolation arrays
Analytical Chemistry, 2006Co-Authors: Dino Di Carlo, Nima Aghdam, Luke P LeeAbstract:High-quality single-Cell data are required for a quantitative systems biology description of Cellular function. However, data of this type are difficult and time-consuming to collect using traditional techniques. We present a robust and simple microfluidic method for trapping single Cells in large arrays to address this problem. Ordered single-Cell Isolation arrays allow for high-density microscopic analysis with simplified image processing. Moreover, for fluorescent assays, on-chip sample preparation (e.g., fluorescent labeling, washing) can be performed, as opposed to manual intensive operations of incubation, centrifugation, and resuspension in previous techniques-saving time and reagents. This technology was applied to determine novel single-Cell enzyme kinetics for three different Cell types (HeLa, 293T, Jurkat). A kinetic model of this process predicted this varied response was due to variation in the concentration of carboxylesterase between Cell types. Nordihydroguaiaretic acid (NDGA) was also characterized as an inhibitor of carboxylesterases. For HeLa Cells, 20 nM of the 50 nM total carboxylesterases was unaffected by NDGA. This type of analysis could be directly applied to quantify a variety of intraCellular enzymes with available fluorogenic substrates.
Heinz Redl - One of the best experts on this subject based on the ideXlab platform.
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Extracorporeal shockwave treatment: A novel tool to improve Schwann Cell Isolation and culture
Cytotherapy, 2016Co-Authors: Christina M.a.p. Schuh, David Hercher, Michaela Stainer, Andreas H. Teuschl, Robert Schmidhammer, Raoul Hopf, Heinz RedlAbstract:Abstract Background aims As new approaches for peripheral nerve regeneration are sought, there is an increasing demand for native Schwann Cells for in vitro testing and/or reimplantation. Extracorporeal shockwave treatment (ESWT) is an emergent technology in the field of regenerative medicine that has also recently been shown to improve peripheral nerve regeneration. Methods In this study, we elucidate the effects of ESWT on Schwann Cell Isolation and culture. Rat sciatic nerves were dissected and treated with ESWT, and Schwann Cells were isolated and cultured for 15 passages. Results Single treatment of the whole nerve ex vivo led to significantly increased extraCellular adenosinetriphosphate as an immediate consequence, and subsequently a number of effects on the culture were observed, starting with a significantly increased Schwann Cell yield after Isolation. In the ESWT group, the quality of culture, reflected in consistently higher purity (S100b, morphology), proliferation rate (5-bromo-2-deoxyuridine, population doublings per passage) and expression of regenerative phenotype-associated markers (P75, glial fibrillary acidic protein, c-Jun), was significantly improved. In contrast, the control group exhibited progressively senescent behavior, reflected in a decrease of proliferation, loss of specific markers and increase in P16INK4A expression. Conclusions ESWT has beneficial effects on Schwann Cell Isolation and culture.
Dino Di Carlo - One of the best experts on this subject based on the ideXlab platform.
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HIGH-THROUGHPUT SIZE BASED RARE Cell Isolation USING MICROSCALE VORTICES
2010Co-Authors: Soojung Claire Hur, Albert J. Mach, Dino Di CarloAbstract:Detection and Isolation of rare Cells from heterogeneous samples is in high demand in the fields of biology, immunology, tissue engineering and medicine. We developed a novel microfluidic device, allowing high-throughput and label-free rare Cell Isolation and enrichment from heterogeneous solution based on Cell size. Utilizing microscale vortices, larger cancer Cells spiked in blood were separated from the sample without the aid of mechanical structures or external forces. Highly viable samples collected off-chip suggest that the proposed technique would be useful for clinical and research application in which in vitro culture is often desired.
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single Cell enzyme concentrations kinetics and inhibition analysis using high density hydrodynamic Cell Isolation arrays
Analytical Chemistry, 2006Co-Authors: Dino Di Carlo, Nima Aghdam, Luke P LeeAbstract:High-quality single-Cell data are required for a quantitative systems biology description of Cellular function. However, data of this type are difficult and time-consuming to collect using traditional techniques. We present a robust and simple microfluidic method for trapping single Cells in large arrays to address this problem. Ordered single-Cell Isolation arrays allow for high-density microscopic analysis with simplified image processing. Moreover, for fluorescent assays, on-chip sample preparation (e.g., fluorescent labeling, washing) can be performed, as opposed to manual intensive operations of incubation, centrifugation, and resuspension in previous techniquessaving time and reagents. This technology was applied to determine novel single-Cell enzyme kinetics for three different Cell types (HeLa, 293T, Jurkat). A kinetic model of this process predicted this varied response was due to variation in the concentration of carboxylesterase between Cell types. Nordihydroguaiaretic acid (NDGA) was also char...
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single Cell enzyme concentrations kinetics and inhibition analysis using high density hydrodynamic Cell Isolation arrays
Analytical Chemistry, 2006Co-Authors: Dino Di Carlo, Nima Aghdam, Luke P LeeAbstract:High-quality single-Cell data are required for a quantitative systems biology description of Cellular function. However, data of this type are difficult and time-consuming to collect using traditional techniques. We present a robust and simple microfluidic method for trapping single Cells in large arrays to address this problem. Ordered single-Cell Isolation arrays allow for high-density microscopic analysis with simplified image processing. Moreover, for fluorescent assays, on-chip sample preparation (e.g., fluorescent labeling, washing) can be performed, as opposed to manual intensive operations of incubation, centrifugation, and resuspension in previous techniques-saving time and reagents. This technology was applied to determine novel single-Cell enzyme kinetics for three different Cell types (HeLa, 293T, Jurkat). A kinetic model of this process predicted this varied response was due to variation in the concentration of carboxylesterase between Cell types. Nordihydroguaiaretic acid (NDGA) was also characterized as an inhibitor of carboxylesterases. For HeLa Cells, 20 nM of the 50 nM total carboxylesterases was unaffected by NDGA. This type of analysis could be directly applied to quantify a variety of intraCellular enzymes with available fluorogenic substrates.
Ki-ho Han - One of the best experts on this subject based on the ideXlab platform.
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Microfluidic technologies for circulating tumor Cell Isolation
The Analyst, 2018Co-Authors: Hyungseok Cho, Jinho Kim, Hanjung Song, Keun Yong Sohn, Minhyon Jeon, Ki-ho HanAbstract:Metastasis is the main cause of tumor-related death, and the dispersal of tumor Cells through the circulatory system is a critical step in the metastatic process. Early detection and analysis of circulating tumor Cells (CTCs) is therefore important for early diagnosis, prognosis, and effective treatment of cancer, enabling favorable clinical outcomes in cancer patients. Accurate and reliable methods for isolating and detecting CTCs are necessary to obtain this clinical information. Over the past two decades, microfluidic technologies have demonstrated great potential for isolating and detecting CTCs from blood. The present paper reviews current advanced microfluidic technologies for isolating CTCs based on various biological and physical principles, and discusses their fundamental advantages and drawbacks for subsequent Cellular and molecular assays. Owing to significant genetic heterogeneity among CTCs, microfluidic technologies for isolating individual CTCs have recently been developed. We discuss these single-Cell Isolation methods, as well as approaches to overcoming the limitations of current microfluidic CTC Isolation technologies. Finally, we provide an overview of future innovative microfluidic platforms.
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Single-Cell Isolation of Circulating Tumor Cells from Whole Blood by Lateral Magnetophoretic Microseparation and Microfluidic Dispensing.
Analytical chemistry, 2016Co-Authors: Jinho Kim, Hyungseok Cho, Song-i Han, Ki-ho HanAbstract:This paper introduces a single-Cell Isolation technology for circulating tumor Cells (CTCs) using a microfluidic device (the “SIM-Chip”). The SIM-Chip comprises a lateral magnetophoretic microseparator and a microdispenser as a two-step cascade platform. First, CTCs were enriched from whole blood by the lateral magnetophoretic microseparator based on immunomagnetic nanobeads. Next, the enriched CTCs were electrically identified by single-Cell impedance cytometer and isolated as single Cells using the microshooter. Using 200 μL of whole blood spiked with 50 MCF7 breast cancer Cells, the analysis demonstrated that the single-Cell Isolation efficiency of the SIM-Chip was 82.4%, and the purity of the isolated MCF7 Cells with respect to WBCs was 92.45%. The data also showed that the WBC depletion rate of the SIM-Chip was 2.5 × 105 (5.4-log). The recovery rates were around 99.78% for spiked MCF7 Cells ranging in number from 10 to 90. The isolated single MCF7 Cells were intact and could be used for subsequent do...
Christina M.a.p. Schuh - One of the best experts on this subject based on the ideXlab platform.
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Extracorporeal shockwave treatment: A novel tool to improve Schwann Cell Isolation and culture
Cytotherapy, 2016Co-Authors: Christina M.a.p. Schuh, David Hercher, Michaela Stainer, Andreas H. Teuschl, Robert Schmidhammer, Raoul Hopf, Heinz RedlAbstract:Abstract Background aims As new approaches for peripheral nerve regeneration are sought, there is an increasing demand for native Schwann Cells for in vitro testing and/or reimplantation. Extracorporeal shockwave treatment (ESWT) is an emergent technology in the field of regenerative medicine that has also recently been shown to improve peripheral nerve regeneration. Methods In this study, we elucidate the effects of ESWT on Schwann Cell Isolation and culture. Rat sciatic nerves were dissected and treated with ESWT, and Schwann Cells were isolated and cultured for 15 passages. Results Single treatment of the whole nerve ex vivo led to significantly increased extraCellular adenosinetriphosphate as an immediate consequence, and subsequently a number of effects on the culture were observed, starting with a significantly increased Schwann Cell yield after Isolation. In the ESWT group, the quality of culture, reflected in consistently higher purity (S100b, morphology), proliferation rate (5-bromo-2-deoxyuridine, population doublings per passage) and expression of regenerative phenotype-associated markers (P75, glial fibrillary acidic protein, c-Jun), was significantly improved. In contrast, the control group exhibited progressively senescent behavior, reflected in a decrease of proliferation, loss of specific markers and increase in P16INK4A expression. Conclusions ESWT has beneficial effects on Schwann Cell Isolation and culture.