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Norman B. Barr - One of the best experts on this subject based on the ideXlab platform.
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molecular identification of Ceratitis capitata diptera tephritidae using dna sequences of the coi barcode region
Annals of The Entomological Society of America, 2012Co-Authors: Norman B. Barr, M. Meyer, Mdsajedul Islam, Bruce A McpheronAbstract:ABSTRACT The utility of the cytochrome oxidase I gene barcode region for diagnosis of the Mediterranean fruit fly, Ceratitis capitata (Weidemann), is evaluated using African fruit fly collections. The method fails to discern C. capitata from its close relative Ceratitis caetrata Munro, based on genetic distances, parsimony networks, or nucleotide diagnostic characters observed in the DNA barcode sequences. When treated as a single taxon, it is possible to discern the C. capitata + C. caetrata lineage from other Ceratitis species. Levels of intraspecific diversity vary within the genus Ceratitis and multiple copies of the mitochondrial gene are reported for Ceratitis cosyra (Walker). The DNA barcoding method based on genetic distance is compared with a molecular identification method using restriction fragment length polymorphism. The DNA barcode and restriction fragment-length polymorphism methods provide similar identification results, but the DNA sequence information is more suitable for quantitative an...
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phylogenetic relationships of Ceratitis fruit flies inferred from nuclear cad and tango arnt gene fragments testing monophyly of the subgenera Ceratitis Ceratitis and c pterandrus
Molecular Phylogenetics and Evolution, 2009Co-Authors: Norman B. Barr, Brian M WiegmannAbstract:Systematic studies of Ceratitis (Tephritidae) fruit flies using molecular (i.e., COI, ND6, and period genes) and morphological (plus host-use characters) data have recently challenged the monophyly of the subgenera Ceratitis (Ceratitis) and Ceratitis (Pterandrus). In this paper, we report on the phylogenetic utility of three single-copy nuclear gene regions (two non-overlapping fragments of the carbamoylphosphate synthetase, CPS, locus of CAD, and a fragment of tango) within these taxa and investigate evolutionary relationships based on a concatenated ca. 3.4 kb data set that includes the six protein encoding gene regions. Results indicate that the CAD and tango genes provide useful phylogenetic signal within the taxa and are compatible with the previously studied genes. The two subgenera, as currently classified, are not monophyletic. Our molecular phylogenetic analyses support a revised classification in which (1) the subgenus C. (Pterandrus) comprises two lineages called A and B, (2) the C. (Pterandrus) B species should be included in C. (Ceratitis), and (3) the newly defined subgenera C. (Pterandrus) (=Pterandrus section A) and C. (Ceratitis) [=C. (Ceratitis) + C. (Pterandrus) section B] are reciprocally monophyletic.
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Phylogenetic relationships of Ceratitis fruit flies inferred from nuclear CAD and tango/ARNT gene fragments: Testing monophyly of the subgenera Ceratitis (Ceratitis) and C. (Pterandrus)
Molecular phylogenetics and evolution, 2009Co-Authors: Norman B. Barr, Brian M WiegmannAbstract:Systematic studies of Ceratitis (Tephritidae) fruit flies using molecular (i.e., COI, ND6, and period genes) and morphological (plus host-use characters) data have recently challenged the monophyly of the subgenera Ceratitis (Ceratitis) and Ceratitis (Pterandrus). In this paper, we report on the phylogenetic utility of three single-copy nuclear gene regions (two non-overlapping fragments of the carbamoylphosphate synthetase, CPS, locus of CAD, and a fragment of tango) within these taxa and investigate evolutionary relationships based on a concatenated ca. 3.4 kb data set that includes the six protein encoding gene regions. Results indicate that the CAD and tango genes provide useful phylogenetic signal within the taxa and are compatible with the previously studied genes. The two subgenera, as currently classified, are not monophyletic. Our molecular phylogenetic analyses support a revised classification in which (1) the subgenus C. (Pterandrus) comprises two lineages called A and B, (2) the C. (Pterandrus) B species should be included in C. (Ceratitis), and (3) the newly defined subgenera C. (Pterandrus) (=Pterandrus section A) and C. (Ceratitis) [=C. (Ceratitis) + C. (Pterandrus) section B] are reciprocally monophyletic.
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Molecular diagnostics of economically important Ceratitis fruit fly species (Diptera: Tephritidae) in Africa using PCR and RFLP analyses
Bulletin of Entomological Research, 2006Co-Authors: Norman B. Barr, H.g. Kibogo, M.k. Billah, Robert S. Copeland, Robert A Wharton, Daniel K Masiga, Ellie O. Osir, M. Meyer, Bruce A McpheronAbstract:The predominantly Afrotropical fruit fly genus Ceratitis contains many species of agricultural importance. Consequently, quarantine of Ceratitis species is a major concern for governmental regulatory agencies. Although diagnostic keys exist for identification of all described Ceratitis species, these tools are based on adult characters. Flies intercepted at ports of entry are usually immatures, and Ceratitis species cannot be diagnosed based on larval morphology. To facilitate identification of Ceratitis pests at ports of entry, this study explores the utility of DNA-based diagnostic tools for a select group of Ceratitis species and related tephritids, some of which infest agriculturally important crops in Africa. The application of the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) method to analyse three mitochondrial genes (12S ribosomal RNA, 16S ribosomal RNA, and NADH-dehydrogenase subunit 6) is sufficient to diagnose 25 species and two species clusters. PCR analysis of the internal transcribed spacerregion1(ITS-1) is able to distinguish three of the five species left unresolved by mitochondrial DNA analysis.
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Molecular phylogenetics of the genus Ceratitis (Diptera: Tephritidae)
Molecular phylogenetics and evolution, 2006Co-Authors: Norman B. Barr, Bruce A McpheronAbstract:The Afrotropical fruit fly genus Ceratitis MacLeay is an economically important group that comprises over 89 species, subdivided into six subgenera. Cladistic analyses of morphological and host use characters have produced several phylogenetic hypotheses for the genus. Only monophyly of the subgenera Pardalaspis and Ceratitis (sensu stricto) and polyphyly of the subgenus Ceratalaspis are common to all of these phylogenies. In this study, the hypotheses developed from morphological and host use characters are tested using gene trees produced from DNA sequence data of two mitochondrial genes (cytochrome oxidase I and NADH-dehydrogenase subunit 6) and a nuclear gene (period). Comparison of gene trees indicates the following relationships: the subgenus Pardalaspis is monophyletic, subsection A of the subgenus Pterandrus is monophyletic, the subgenus Pterandrus may be either paraphyletic or polyphyletic, the subgenus Ceratalaspis is polyphyletic, and the subgenus Ceratitis s. s. might not be monophyletic. In addition, the genera Ceratitis and Trirhithrum do not form reciprocally monophyletic clades in the gene trees. Although the data statistically reject monophyly for Trirhithrum under the Shimodaira-Hasegawa test, they do not reject monophyly of Ceratitis.
M. Meyer - One of the best experts on this subject based on the ideXlab platform.
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molecular identification of Ceratitis capitata diptera tephritidae using dna sequences of the coi barcode region
Annals of The Entomological Society of America, 2012Co-Authors: Norman B. Barr, M. Meyer, Mdsajedul Islam, Bruce A McpheronAbstract:ABSTRACT The utility of the cytochrome oxidase I gene barcode region for diagnosis of the Mediterranean fruit fly, Ceratitis capitata (Weidemann), is evaluated using African fruit fly collections. The method fails to discern C. capitata from its close relative Ceratitis caetrata Munro, based on genetic distances, parsimony networks, or nucleotide diagnostic characters observed in the DNA barcode sequences. When treated as a single taxon, it is possible to discern the C. capitata + C. caetrata lineage from other Ceratitis species. Levels of intraspecific diversity vary within the genus Ceratitis and multiple copies of the mitochondrial gene are reported for Ceratitis cosyra (Walker). The DNA barcoding method based on genetic distance is compared with a molecular identification method using restriction fragment length polymorphism. The DNA barcode and restriction fragment-length polymorphism methods provide similar identification results, but the DNA sequence information is more suitable for quantitative an...
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Molecular diagnostics of economically important Ceratitis fruit fly species (Diptera: Tephritidae) in Africa using PCR and RFLP analyses
Bulletin of Entomological Research, 2006Co-Authors: Norman B. Barr, H.g. Kibogo, M.k. Billah, Robert S. Copeland, Robert A Wharton, Daniel K Masiga, Ellie O. Osir, M. Meyer, Bruce A McpheronAbstract:The predominantly Afrotropical fruit fly genus Ceratitis contains many species of agricultural importance. Consequently, quarantine of Ceratitis species is a major concern for governmental regulatory agencies. Although diagnostic keys exist for identification of all described Ceratitis species, these tools are based on adult characters. Flies intercepted at ports of entry are usually immatures, and Ceratitis species cannot be diagnosed based on larval morphology. To facilitate identification of Ceratitis pests at ports of entry, this study explores the utility of DNA-based diagnostic tools for a select group of Ceratitis species and related tephritids, some of which infest agriculturally important crops in Africa. The application of the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) method to analyse three mitochondrial genes (12S ribosomal RNA, 16S ribosomal RNA, and NADH-dehydrogenase subunit 6) is sufficient to diagnose 25 species and two species clusters. PCR analysis of the internal transcribed spacerregion1(ITS-1) is able to distinguish three of the five species left unresolved by mitochondrial DNA analysis.
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Regular ArticleSystematic revision of the subgenus Ceratitis MacLeay s.s. (Diptera, Tephritidae)☆
Zoological Journal of the Linnean Society, 2000Co-Authors: M. MeyerAbstract:The fruit fly genus Ceratitis MacLeay (Diptera, Tephritidae) comprises several important pest species attacking a wide range of unrelated fruits. This is the third paper in a series revising the genus, and deals with representatives of the subgenus Ceratitis s.s. Eight species are recognized Ceratitis brachychaeta Freidberg,C. caetrata Munro, C. capitata (Wiedemann), C. catoirii Guerin-Meneville, C. cornuta (Bezzi), C. malgassa Munro, C. manjakatompo Hancock and C. pinax Munro. Lectotypes are designated for C. catoirii and C. pinax. A key for both sexes is provided, with illustrations of cephalic bristles, mesonotal and wing patterns, and shape of aculeus. All species are Afrotropical, except for C. capitata, which has become widespread. The phylogeny and biogeography of Ceratitis s.s. are discussed, based on a cladistic analysis, and showing indications that the mediterranean fruit fly, C. capitata, may have its origins in eastern Africa. The relationship of this subgenus within the genus Ceratitis s.l. is also outlined.
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Systematic revision of the subgenus Ceratitis MacLeay s.s. (Diptera, Tephritidae)
Zoological Journal of the Linnean Society, 2000Co-Authors: M. MeyerAbstract:Abstract The fruit fly genus Ceratitis MacLeay (Diptera, Tephritidae) comprises several important pest species attacking a wide range of unrelated fruits. This is the third paper in a series revising the genus, and deals with representatives of the subgenus Ceratitis s.s. Eight species are recognized Ceratitis brachychaeta Freidberg, C. caetrata Munro, C. capitata (Wiedemann), C. catoirii Guerin-Meneville, C. cornuta (Bezzi), C. malgassa Munro, C. manjakatompo Hancock and C. pinax Munro. Lectotypes are designated for C. catoirii and C. pinax . A key for both sexes is provided, with illustrations of cephalic bristles, mesonotal and wing patterns, and shape of aculeus. All species are Afrotropical, except for C. capitata , which has become widespread. The phylogeny and biogeography of Ceratitis s.s. are discussed, based on a cladistic analysis, and showing indications that the mediterranean fruit fly, C. capitata , may have its origins in eastern Africa. The relationship of this subgenus within the genus Ceratitis s.l. is also outlined.
Brian M Wiegmann - One of the best experts on this subject based on the ideXlab platform.
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phylogenetic relationships of Ceratitis fruit flies inferred from nuclear cad and tango arnt gene fragments testing monophyly of the subgenera Ceratitis Ceratitis and c pterandrus
Molecular Phylogenetics and Evolution, 2009Co-Authors: Norman B. Barr, Brian M WiegmannAbstract:Systematic studies of Ceratitis (Tephritidae) fruit flies using molecular (i.e., COI, ND6, and period genes) and morphological (plus host-use characters) data have recently challenged the monophyly of the subgenera Ceratitis (Ceratitis) and Ceratitis (Pterandrus). In this paper, we report on the phylogenetic utility of three single-copy nuclear gene regions (two non-overlapping fragments of the carbamoylphosphate synthetase, CPS, locus of CAD, and a fragment of tango) within these taxa and investigate evolutionary relationships based on a concatenated ca. 3.4 kb data set that includes the six protein encoding gene regions. Results indicate that the CAD and tango genes provide useful phylogenetic signal within the taxa and are compatible with the previously studied genes. The two subgenera, as currently classified, are not monophyletic. Our molecular phylogenetic analyses support a revised classification in which (1) the subgenus C. (Pterandrus) comprises two lineages called A and B, (2) the C. (Pterandrus) B species should be included in C. (Ceratitis), and (3) the newly defined subgenera C. (Pterandrus) (=Pterandrus section A) and C. (Ceratitis) [=C. (Ceratitis) + C. (Pterandrus) section B] are reciprocally monophyletic.
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Phylogenetic relationships of Ceratitis fruit flies inferred from nuclear CAD and tango/ARNT gene fragments: Testing monophyly of the subgenera Ceratitis (Ceratitis) and C. (Pterandrus)
Molecular phylogenetics and evolution, 2009Co-Authors: Norman B. Barr, Brian M WiegmannAbstract:Systematic studies of Ceratitis (Tephritidae) fruit flies using molecular (i.e., COI, ND6, and period genes) and morphological (plus host-use characters) data have recently challenged the monophyly of the subgenera Ceratitis (Ceratitis) and Ceratitis (Pterandrus). In this paper, we report on the phylogenetic utility of three single-copy nuclear gene regions (two non-overlapping fragments of the carbamoylphosphate synthetase, CPS, locus of CAD, and a fragment of tango) within these taxa and investigate evolutionary relationships based on a concatenated ca. 3.4 kb data set that includes the six protein encoding gene regions. Results indicate that the CAD and tango genes provide useful phylogenetic signal within the taxa and are compatible with the previously studied genes. The two subgenera, as currently classified, are not monophyletic. Our molecular phylogenetic analyses support a revised classification in which (1) the subgenus C. (Pterandrus) comprises two lineages called A and B, (2) the C. (Pterandrus) B species should be included in C. (Ceratitis), and (3) the newly defined subgenera C. (Pterandrus) (=Pterandrus section A) and C. (Ceratitis) [=C. (Ceratitis) + C. (Pterandrus) section B] are reciprocally monophyletic.
Bruce A Mcpheron - One of the best experts on this subject based on the ideXlab platform.
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molecular identification of Ceratitis capitata diptera tephritidae using dna sequences of the coi barcode region
Annals of The Entomological Society of America, 2012Co-Authors: Norman B. Barr, M. Meyer, Mdsajedul Islam, Bruce A McpheronAbstract:ABSTRACT The utility of the cytochrome oxidase I gene barcode region for diagnosis of the Mediterranean fruit fly, Ceratitis capitata (Weidemann), is evaluated using African fruit fly collections. The method fails to discern C. capitata from its close relative Ceratitis caetrata Munro, based on genetic distances, parsimony networks, or nucleotide diagnostic characters observed in the DNA barcode sequences. When treated as a single taxon, it is possible to discern the C. capitata + C. caetrata lineage from other Ceratitis species. Levels of intraspecific diversity vary within the genus Ceratitis and multiple copies of the mitochondrial gene are reported for Ceratitis cosyra (Walker). The DNA barcoding method based on genetic distance is compared with a molecular identification method using restriction fragment length polymorphism. The DNA barcode and restriction fragment-length polymorphism methods provide similar identification results, but the DNA sequence information is more suitable for quantitative an...
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Molecular diagnostics of economically important Ceratitis fruit fly species (Diptera: Tephritidae) in Africa using PCR and RFLP analyses
Bulletin of Entomological Research, 2006Co-Authors: Norman B. Barr, H.g. Kibogo, M.k. Billah, Robert S. Copeland, Robert A Wharton, Daniel K Masiga, Ellie O. Osir, M. Meyer, Bruce A McpheronAbstract:The predominantly Afrotropical fruit fly genus Ceratitis contains many species of agricultural importance. Consequently, quarantine of Ceratitis species is a major concern for governmental regulatory agencies. Although diagnostic keys exist for identification of all described Ceratitis species, these tools are based on adult characters. Flies intercepted at ports of entry are usually immatures, and Ceratitis species cannot be diagnosed based on larval morphology. To facilitate identification of Ceratitis pests at ports of entry, this study explores the utility of DNA-based diagnostic tools for a select group of Ceratitis species and related tephritids, some of which infest agriculturally important crops in Africa. The application of the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) method to analyse three mitochondrial genes (12S ribosomal RNA, 16S ribosomal RNA, and NADH-dehydrogenase subunit 6) is sufficient to diagnose 25 species and two species clusters. PCR analysis of the internal transcribed spacerregion1(ITS-1) is able to distinguish three of the five species left unresolved by mitochondrial DNA analysis.
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Molecular phylogenetics of the genus Ceratitis (Diptera: Tephritidae)
Molecular phylogenetics and evolution, 2006Co-Authors: Norman B. Barr, Bruce A McpheronAbstract:The Afrotropical fruit fly genus Ceratitis MacLeay is an economically important group that comprises over 89 species, subdivided into six subgenera. Cladistic analyses of morphological and host use characters have produced several phylogenetic hypotheses for the genus. Only monophyly of the subgenera Pardalaspis and Ceratitis (sensu stricto) and polyphyly of the subgenus Ceratalaspis are common to all of these phylogenies. In this study, the hypotheses developed from morphological and host use characters are tested using gene trees produced from DNA sequence data of two mitochondrial genes (cytochrome oxidase I and NADH-dehydrogenase subunit 6) and a nuclear gene (period). Comparison of gene trees indicates the following relationships: the subgenus Pardalaspis is monophyletic, subsection A of the subgenus Pterandrus is monophyletic, the subgenus Pterandrus may be either paraphyletic or polyphyletic, the subgenus Ceratalaspis is polyphyletic, and the subgenus Ceratitis s. s. might not be monophyletic. In addition, the genera Ceratitis and Trirhithrum do not form reciprocally monophyletic clades in the gene trees. Although the data statistically reject monophyly for Trirhithrum under the Shimodaira-Hasegawa test, they do not reject monophyly of Ceratitis.
Romano Dallai - One of the best experts on this subject based on the ideXlab platform.
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The ceratotoxin gene family in the medfly Ceratitis capitata and the Natal fruit fly Ceratitis rosa (Diptera: Tephritidae).
Heredity, 2003Co-Authors: Marco Rosetto, Silvia Ciolfi, Tiziana De Filippis, Daniela Marchini, Francesco Frati, Serge Quilici, Romano DallaiAbstract:The ceratotoxin gene family in the medfly Ceratitis capitata and the Natal fruit fly Ceratitis rosa (Diptera: Tephritidae)