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Thomas C. Harrington - One of the best experts on this subject based on the ideXlab platform.
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Genetic Bottlenecks for Two Populations of Ceratocystis fimbriata on Sweet Potato and Pomegranate in China
Plant disease, 2016Co-Authors: Thomas C. Harrington, Q. Huang, Douglas Mcnew, Y. M. Somasekhara, Acelino C AlfenasAbstract:Chinese isolates of Ceratocystis fimbriata from sweet potato (Ipomoea batatas) and pomegranate (Punica granatum) were genetically compared with a worldwide collection of isolates from a variety of hosts. Isolates from black-rotted storage roots of sweet potato in China, Japan, Australasia, and the United States had identical internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequences and only minor variation in microsatellite alleles. Sequences of their mating type genes were most similar to those of isolates from various hosts in Ecuador, a center of diversity for sweet potato. Isolates from Colocasia esculenta (taro) and pomegranate from Yunnan and Sichuan had only one ITS rDNA sequence (haplotype ITS5). This haplotype, sequences of mating type genes, and microsatellite alleles linked these isolates to isolates from Eucalyptus stumps in South China and diseased Eucalyptus trees in Brazil, supporting the hypothesis that the pomegranate population originated from Brazil via cuttings of Eucalyptus. Is...
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Genetic variation, morphology and pathogenicity of Ceratocystis fimbriata on Hevea brasiliensis in Brazil
Tropical Plant Pathology, 2015Co-Authors: Denise C. O. F. Valdetaro, Thomas C. Harrington, Maria A. Ferreira, Leonardo S. S. Oliveira, Lúcio M. S. Guimarães, Rodrigo G. Freitas, Acelino C AlfenasAbstract:Ceratocystis fimbriata causes diseases on a wide variety of plants in Brazil, including rubber tree ( Hevea brasiliensis ), on which it causes gray mold or moldy rot on tapping panels affecting latex yield. However, C. fimbriata isolated from rubber tree have not been critically studied. Phylogenetic analyses of sequences of ITS rDNA and a mating type gene placed rubber tree isolates from Acre and Bahia among Brazilian isolates of C. fimbriata from other hosts. In the analyses of 14 microsatellite loci, the rubber tree isolates from Bahia were identical to each other and had alleles similar to those of Brazilian isolates from mango and eucalyptus. The microsatellite alleles of the Acre rubber tree isolates were identical to each other but distinct from other Brazilian isolates. The rubber tree isolates were morphologically indistinguishable from each other and very similar to the isolates of C. fimbriata on Ipomoea batatas , on which the species was originally described. Based on inoculation experiments results, the Bahia and Acre rubber tree isolates do not appear to be host specialized, which is typical for Brazilian isolates of C. fimbriata sensu stricto.
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Annona squamosa, a New Host of Ceratocystis fimbriata
2015Co-Authors: Silvaldo Felipe Da Silveira, Thomas C. Harrington, Acelino C Alfenas, Vicente Mussi-dias, Christine J. B. Engelbrecht, Carolina R SilvaAbstract:Annona squamosa, a new host of Ceratocystis fimbriata. Fitopatologia Brasileira 31:394-397. 2006. Mango branch blight disease, caused by Ceratocystis fimbriata, is endemic to the municipality of São Fidelis in northern Rio de Janeiro State. In addition to mango, C. fimbriata was found associated with sugar apple trees (Annona squamosa) showing symptoms of branch blight in São Fidelis. Sugar apple and mango isolates from the same region had the same morphology and showed similar ITS-rDNA sequences. These sequences were also similar to other Brazilian isolates of C. fimbriata sensu stricto. Cross inoculation of such isolates obtained from diseased sugar apple and mango resulted in diseased symptoms on both plant species. This is the first record of A. squamosa as a host for C. fimbriata
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Short title: Ceratocystis fimbriata ITS Intraspecific and intragenomic variability of ITS rDNA sequences reveals taxonomic problems in Ceratocystis fimbriata sensu stricto
2014Co-Authors: Thomas C. Harrington, M. R. Kazmi, Abdullah M. Al-sadiAbstract:50011 Abstract: Fourteen new species in the Latin American Clade (LAC) of the Ceratocystis fimbriata complex recently were distinguished from C. fimbriata sensu stricto largely based on variation in ITS rDNA sequences. Among the 116 isolates representing the LAC, there were 41 ITS haplotypes. Maximum parsimony (MP) analysis of ITS sequences produced poorly resolved trees. In contrast, analyses of mating-type genes (MAT1-1-2 and MAT1-2-1) resolved a single MP tree with branches of high bootstrap and posterior probability support. Four isolates showed intragenomic variation in ITS sequences. Cloning and sequencing of PCR products from a single haploid strain
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Movement of genotypes of Ceratocystis fimbriata within and among Eucalyptus plantations in Brazil.
Phytopathology, 2011Co-Authors: Maria A. Ferreira, Thomas C. Harrington, Acelino C Alfenas, Eduardo S. G. MizubutiAbstract:Ferreira, M. A., Harrington, T. C., Alfenas, A. C., and Mizubuti, E. S. G. 2011. Movement of genotypes of Ceratocystis fimbriata within and among Eucalyptus plantations in Brazil. Phytopathology 101:1005-1012. Ceratocystis wilt on eucalyptus, caused by Ceratocystis fimbriata, was first recognized in 1997 in the state of Bahia, Brazil, but is now known in five other states and in four other countries. C. fimbriata is a native, soilborne pathogen in some parts of Brazil but we hypothesized that genotypes of the pathogen have been moved among plantations in rooted cuttings collected from diseased trees and within plantations on cutting tools. We used six microsatellite markers to identify 78 genotypes of C. fimbriata among 177 isolates from individual trees in 20 eucalyptus plantations. The highest gene and genotypic diversity values were found in plantations on formerly wild Cerrado forest in Minas Gerais, suggesting that the fungus was in the soil prior to planting eucalyptus. In contrast, one or only a few genotypes were found in plantations on previous pastureland (with no woody hosts) in Bahia and Sao Paulo, and most of these genotypes were found in a Bahian nursery or in one of two Bahian plantations that were sources for rooted cuttings. Sources of cuttings tended to be dominated by one or a few genotypes that may have been spread within the plantation on cutting tools. Additional keywords: Atlantic rainforest.
Acelino C Alfenas - One of the best experts on this subject based on the ideXlab platform.
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Genetic Bottlenecks for Two Populations of Ceratocystis fimbriata on Sweet Potato and Pomegranate in China
Plant disease, 2016Co-Authors: Thomas C. Harrington, Q. Huang, Douglas Mcnew, Y. M. Somasekhara, Acelino C AlfenasAbstract:Chinese isolates of Ceratocystis fimbriata from sweet potato (Ipomoea batatas) and pomegranate (Punica granatum) were genetically compared with a worldwide collection of isolates from a variety of hosts. Isolates from black-rotted storage roots of sweet potato in China, Japan, Australasia, and the United States had identical internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequences and only minor variation in microsatellite alleles. Sequences of their mating type genes were most similar to those of isolates from various hosts in Ecuador, a center of diversity for sweet potato. Isolates from Colocasia esculenta (taro) and pomegranate from Yunnan and Sichuan had only one ITS rDNA sequence (haplotype ITS5). This haplotype, sequences of mating type genes, and microsatellite alleles linked these isolates to isolates from Eucalyptus stumps in South China and diseased Eucalyptus trees in Brazil, supporting the hypothesis that the pomegranate population originated from Brazil via cuttings of Eucalyptus. Is...
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Genetic variation, morphology and pathogenicity of Ceratocystis fimbriata on Hevea brasiliensis in Brazil
Tropical Plant Pathology, 2015Co-Authors: Denise C. O. F. Valdetaro, Thomas C. Harrington, Maria A. Ferreira, Leonardo S. S. Oliveira, Lúcio M. S. Guimarães, Rodrigo G. Freitas, Acelino C AlfenasAbstract:Ceratocystis fimbriata causes diseases on a wide variety of plants in Brazil, including rubber tree ( Hevea brasiliensis ), on which it causes gray mold or moldy rot on tapping panels affecting latex yield. However, C. fimbriata isolated from rubber tree have not been critically studied. Phylogenetic analyses of sequences of ITS rDNA and a mating type gene placed rubber tree isolates from Acre and Bahia among Brazilian isolates of C. fimbriata from other hosts. In the analyses of 14 microsatellite loci, the rubber tree isolates from Bahia were identical to each other and had alleles similar to those of Brazilian isolates from mango and eucalyptus. The microsatellite alleles of the Acre rubber tree isolates were identical to each other but distinct from other Brazilian isolates. The rubber tree isolates were morphologically indistinguishable from each other and very similar to the isolates of C. fimbriata on Ipomoea batatas , on which the species was originally described. Based on inoculation experiments results, the Bahia and Acre rubber tree isolates do not appear to be host specialized, which is typical for Brazilian isolates of C. fimbriata sensu stricto.
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Annona squamosa, a New Host of Ceratocystis fimbriata
2015Co-Authors: Silvaldo Felipe Da Silveira, Thomas C. Harrington, Acelino C Alfenas, Vicente Mussi-dias, Christine J. B. Engelbrecht, Carolina R SilvaAbstract:Annona squamosa, a new host of Ceratocystis fimbriata. Fitopatologia Brasileira 31:394-397. 2006. Mango branch blight disease, caused by Ceratocystis fimbriata, is endemic to the municipality of São Fidelis in northern Rio de Janeiro State. In addition to mango, C. fimbriata was found associated with sugar apple trees (Annona squamosa) showing symptoms of branch blight in São Fidelis. Sugar apple and mango isolates from the same region had the same morphology and showed similar ITS-rDNA sequences. These sequences were also similar to other Brazilian isolates of C. fimbriata sensu stricto. Cross inoculation of such isolates obtained from diseased sugar apple and mango resulted in diseased symptoms on both plant species. This is the first record of A. squamosa as a host for C. fimbriata
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Movement of genotypes of Ceratocystis fimbriata within and among Eucalyptus plantations in Brazil.
Phytopathology, 2011Co-Authors: Maria A. Ferreira, Thomas C. Harrington, Acelino C Alfenas, Eduardo S. G. MizubutiAbstract:Ferreira, M. A., Harrington, T. C., Alfenas, A. C., and Mizubuti, E. S. G. 2011. Movement of genotypes of Ceratocystis fimbriata within and among Eucalyptus plantations in Brazil. Phytopathology 101:1005-1012. Ceratocystis wilt on eucalyptus, caused by Ceratocystis fimbriata, was first recognized in 1997 in the state of Bahia, Brazil, but is now known in five other states and in four other countries. C. fimbriata is a native, soilborne pathogen in some parts of Brazil but we hypothesized that genotypes of the pathogen have been moved among plantations in rooted cuttings collected from diseased trees and within plantations on cutting tools. We used six microsatellite markers to identify 78 genotypes of C. fimbriata among 177 isolates from individual trees in 20 eucalyptus plantations. The highest gene and genotypic diversity values were found in plantations on formerly wild Cerrado forest in Minas Gerais, suggesting that the fungus was in the soil prior to planting eucalyptus. In contrast, one or only a few genotypes were found in plantations on previous pastureland (with no woody hosts) in Bahia and Sao Paulo, and most of these genotypes were found in a Bahian nursery or in one of two Bahian plantations that were sources for rooted cuttings. Sources of cuttings tended to be dominated by one or a few genotypes that may have been spread within the plantation on cutting tools. Additional keywords: Atlantic rainforest.
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annona squamosa a new host of Ceratocystis fimbriata
Fitopatologia Brasileira, 2006Co-Authors: Silvaldo Felipe Da Silveira, Thomas C. Harrington, Vicente Mussidias, Christine Engelbrecht, Acelino C Alfenas, Carolina R SilvaAbstract:Mango branch blight disease, caused by Ceratocystis fimbriata , is endemic to the municipality of Sao Fidelis in northern Rio de Janeiro State. In addition to mango, C. fimbriata was found associated with sugar apple trees (Annona squamosa) showing symptoms of branch blight in Sao Fidelis. Sugar apple and mango isolates from the same region had the same morphology and showed similar ITS-rDNA sequences. These sequences were also similar to other Brazilian isolates of C. fimbriata sensu stricto. Cross inoculation of such isolates obtained from diseased sugar apple and mango resulted in diseased symptoms on both plant species. This is the first record of A. squamosa as a host for C. fimbriata . Additional keywords: Host range, sugar apple, Mangifera indica, pathogenic variability. RESUMO Annona squamosa, nova hospedeira de Ceratocystis fimbriata . A seca-da-mangueira, causada pelo fungo Ceratocystis fimbriata , e uma doenca endemica no municipio de Sao Fidelis, no norte do Estado do Rio de Janeiro. Ceratocystis fimbriata foi encontrado associado a arvores de pinha (Annona squamosa) apresentando sintomas de seca dos ramos. Isolados obtidos de pinha e manga, originarios da mesma regiao, apresentaram a mesma morfologia e sequencias espacadoras ITS do rDNA similares. Estas sequencias foram tambem similares a de outros isolados brasileiros de C. fimbriata sensu stricto. Inoculacoes cruzadas dos isolados de pinha e manga resultaram no desenvolvimento de sintomas em ambas especies de plantas. Este e o primeiro relato de A. squamosa como hospedeira de C. fimbriata . Palavras-chave adicionais: Gama de hospedeiros, pinha, Mangifera indica, variabilidade patogenica.
Brenda D. Wingfield - One of the best experts on this subject based on the ideXlab platform.
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ima genome f 11 draft genome sequences of fusarium xylarioides teratosphaeria gauchensis and t zuluensis and genome annotation for Ceratocystis fimbriata
IMA Fungus, 2019Co-Authors: Brenda D. Wingfield, Irene Barnes, Arista Fourie, Melissa Simpson, Vuyiswa Bushulanjah, Janneke Aylward, Martin Petrus Albertus Coetzee, L L Dreyer, Tuan A Duong, David M GeiserAbstract:Draft genomes of the fungal species Fusarium xylarioides, Teratosphaeria gauchensis and T. zuluensis are presented. In addition an annotation of the genome of Ceratocystis fimbriata is presented. Overall these genomes provide a valuable resource for understanding the molecular processes underlying pathogenicity and potential management strategies of these economically important fungi.
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IMA Genome-F 1: Ceratocystis fimbriata: Draft nuclear genome sequence for the plant pathogen, Ceratocystis fimbriata.
IMA fungus, 2013Co-Authors: P. Markus Wilken, Emma Theodora Steenkamp, Michael J. Wingfield, Z. Wilhelm De Beer, Brenda D. WingfieldAbstract:The draft nuclear genome of Ceratocystis fimbriata, the type species of Ceratocystis, is comprised of 29 410 862 bp. De novo gene prediction produced 7 266 genes, which is low for an ascomycete fungus. The availability of the genome provides opportunities to study aspects of the biology of this and other Ceratocystis species.
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Analysis of microsatellite markers in the genome of the plant pathogen Ceratocystis fimbriata.
Fungal Biology, 2013Co-Authors: Melissa C Simpson, P. Markus Wilken, Michael J. Wingfield, Martin P A Coetzee, Brenda D. WingfieldAbstract:Ceratocystis fimbriata sensu lato represents a complex of cryptic and commonly plant pathogenic species that are morphologically similar. Species in this complex have been described using morphological characteristics, intersterility tests and phylogenetics. Microsatellite markers have been useful to study the population structure and origin of some species in the complex. In this study we sequenced the genome of C. fimbriata. This provided an opportunity to mine the genome for microsatellites, to develop new microsatellite markers, and map previously developed markers onto the genome. Over 6000 microsatellites were identified in the genome and their abundance and distribution was determined. Ceratocystis fimbriata has a medium level of microsatellite density and slightly smaller genome when compared with other fungi for which similar microsatellite analyses have been performed. This is the first report of a microsatellite analysis conducted on a genome sequence of a fungal species in the order Microascales. Forty-seven microsatellite markers have been published for population genetic studies, of which 35 could be mapped onto the C. fimbriata genome sequence. We developed an additional ten microsatellite markers within putative genes to differentiate between species in the C. fimbriata s.l. complex. These markers were used to distinguish between 12 species in the complex.
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DNA based characterization of Ceratocystis fimbriata isolates associated with mango decline in Oman
Australasian Plant Pathology, 2005Co-Authors: M. Van Wyk, Brenda D. Wingfield, Ali O. Al-adawi, A. M. Al-subhi, M. L. Deadman, Michael J. WingfieldAbstract:A serious mango decline disease has recently been reported from the Sultanate of Oman. Based on morphological characteristics, the pathogen responsible for the disease was previously identified as Ceratocystis fimbriata. C. fimbriata is one of the most important pathogens of mango in Brazil and its appearance in Oman is of great concern. Recent phylogenetic studies have shown that C. fimbriata most likely represents a species complex. The aim of this study was to confirm the identity of the mango pathogen in Oman based on DNA sequences. Sequence data were obtained for the internal transcribed spacer 1 and 2 regions and the 5.8S rRNA gene regions and these were compared with sequence data of C. fimbriata from several hosts and geographic areas. The isolates from Oman where shown to represent C. fimbriata sensu lato and also to be most closely related to an isolate from mango in Brazil. This provides some evidence that the mango pathogen in Oman might have originated in Brazil.
David M Geiser - One of the best experts on this subject based on the ideXlab platform.
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ima genome f 11 draft genome sequences of fusarium xylarioides teratosphaeria gauchensis and t zuluensis and genome annotation for Ceratocystis fimbriata
IMA Fungus, 2019Co-Authors: Brenda D. Wingfield, Irene Barnes, Arista Fourie, Melissa Simpson, Vuyiswa Bushulanjah, Janneke Aylward, Martin Petrus Albertus Coetzee, L L Dreyer, Tuan A Duong, David M GeiserAbstract:Draft genomes of the fungal species Fusarium xylarioides, Teratosphaeria gauchensis and T. zuluensis are presented. In addition an annotation of the genome of Ceratocystis fimbriata is presented. Overall these genomes provide a valuable resource for understanding the molecular processes underlying pathogenicity and potential management strategies of these economically important fungi.
Chengling Zhang - One of the best experts on this subject based on the ideXlab platform.
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Characterization of a novel chitinase from sweet potato and its fungicidal effect against Ceratocystis fimbriata
Journal of agricultural and food chemistry, 2020Co-Authors: Meiyan Liu, Gong Ying, Houjun Sun, Jian Zhang, Liming Zhang, Jian Sun, Yonghua Han, Huang Jinjin, Chengling ZhangAbstract:Black rot, caused by Ceratocystis fimbriata, is a destructive disease of sweet potato (Ipomoea batatas). In this study, a novel chitinase (IbChiA) was screened from sweet potato, which showed a rem...