The Experts below are selected from a list of 2070 Experts worldwide ranked by ideXlab platform

Luciana Sonne - One of the best experts on this subject based on the ideXlab platform.

  • Virome of crab-eating (Cerdocyon Thous) and pampas foxes (Lycalopex gymnocercus) from southern Brazil and Uruguay.
    Infection genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 2020
    Co-Authors: M. N. Weber, Joao Fabio Soares, Renata Da Fontoura Budaszewski, A. C. S. Mósena, M. S. Da Silva, R. Canova, C. De Lorenzo, J.c. Olegário, L. F. Baumbach, Luciana Sonne
    Abstract:

    Abstract Crab-eating (Cerdocyon Thous) and Pampas foxes (Lycalopex gymnocercus) are wild canids distributed in South America. Domestic dogs (Canis lupus familiaris) and wild canids may share viral pathogens, including rabies virus (RABV), canine distemper virus (CDV), and canine parvovirus 2 (CPV-2). To characterize the virome of these wild canid species, the present work evaluated the spleen and mesenteric lymph node virome of 17 crab-eating and five Pampas foxes using high-throughput sequencing (HTS). Organ samples were pooled and sequenced using an Illumina MiSeq platform. Additional PCR analyses were performed to identify the frequencies and host origin for each virus detected by HTS. Sequences more closely related to the Paramyxoviridae, Parvoviridae and Anelloviridae families were detected, as well as circular Rep-encoding single-stranded (CRESS) DNA viruses. CDV was found only in crab-eating foxes, whereas CPV-2 was found in both canid species; both viruses were closely related to sequences reported in domestic dogs from southern Brazil. Moreover, the present work reported the detection of canine bocavirus (CBoV) strains that were genetically divergent from CBoV-1 and 2 lineages. Finally, we also characterized CRESS DNA viruses and anelloviruses with marked diversity. The results of this study contribute to the body of knowledge regarding wild canid viruses that can potentially be shared with domestic canids or other species.

  • Presentation of hemolytic and hemorrhagic rangeliosis in Cerdocyon Thous
    Ticks and tick-borne diseases, 2019
    Co-Authors: Bruna Copat, Luciana Sonne, Paulo Vinicius Bastiani, Fernanda Castellarin Jaconi, Wanderley Wallyson Damarem, André Felipe Streck, Eduardo Conceição De Oliveira, Raqueli T. França
    Abstract:

    Abstract Rangeliosis, caused by protozoan Rangelia vitalii, is transmitted by the tick Amblyomma aureolatum. The disease is characterized by hemolytic and hemorrhagic disorder and has been described in dogs and other wild canids. The aim of this study was to compare clinicopathological findings and laboratory results of a Rangelia infection in a crab-eating fox (Cerdocyon Thous) with those of canine rangeliosis. The zoo of Universidade de Caxias do Sul, received a crab-eating fox with marked jaundice in mucous membranes, dark-colored stools and neurological signs. The animal underwent an ear tip smear examination and blood collection for complete blood counts, serum biochemistry and PCR. Free-living and intraerythrocytic pyriform structures consistent with R. vitalii were found in the blood smear of the ear tip. The erythrogram revealed normocytic normochromic anemia, moderate macrocytosis, polychromasia and metarubricytosis. The leukogram revealed leukocytosis with neutrophilia and monocytosis, as well as severe thrombocytopenia. Serum biochemistry showed hypoproteinemia, hypoalbuminemia and elevated levels of urea and creatinine. The treatment was performed with imidocarb hydrochloride and dexamethasone, however 24 h after initiation of treatment the animal died. Macroscopic examination revealed jaundice, subcutaneous edema, enlarged superficial lymph nodes, splenomegaly, and hemorrhage of internal organs. Histological sections of the cerebellum, lung, pancreas, intestine and heart were consistent with R. vitalii infection of the vascular endothelium. Pathological and hematological findings were similar to those found in infected dogs, with clinical presentation characterized by hemolytic anemia and hemorrhage. The description of this case showed that C. Thous does not only serve as reservoir of R. vitalii but may also develop disease.

  • Angiostrongylosis in Cerdocyon Thous (crab-eating fox) and Lycalopex gymnocercus (Pampas fox) in Southern Brazil.
    Parasitology, 2018
    Co-Authors: Rafaela Albuquerque Caprioli, Joao Fabio Soares, Caroline Pinto De Andrade, David Driemeier, Fernando Froner Argenta, Luiza Presser Ehlers, Saulo Petinatti Pavarini, Luciana Sonne
    Abstract:

    The objective of this study was to identify species of Angiostrongylus spp. infecting wild carnivores in Southern Brazil, as well as to describe gross and histopathological findings associated with the infection. Necropsy was conducted in 16 wild carnivores parasitized by Angiostrongylus spp. Analysed lungs revealed multifocal dark-red areas of consolidation; in one case, multifocal firm white nodules spread in all pulmonary lobes were observed. In one animal, a focally extensive area of malacia associated with haemorrhage was noted in the encephalon. Histologically, multifocal granulomatous pneumonia or bronchopneumonia, associated with eggs and larvae in blood vessels, lung interstitium, alveoli, and sometimes in bronchi and bronchioles was observed. Adult nematodes were seen within blood vessels. The lesion observed in the brain was characterized as a focally extensive area of malacia associated with gitter cells, haemorrhage, thrombosis and a free intralesional larva. Through molecular techniques, seven positive samples of Angiostrongylus cantonensis were obtained, including the brain sample, and a positive sample of Angiostrongylus vasorum-like, all in Cerdocyon Thous. The positive sample for A. vasorum showed 97% similarity with sequences deposited in GenBank, suggesting a new species or subspecies of Angiostrongylus sp. Infection of Lycalopex gymnocercus by Angiostrongylus spp. was confirmed by histological evaluation.

  • Mamastrovirus 5 detected in a crab-eating fox (Cerdocyon Thous): Expanding wildlife host range of astroviruses.
    Comparative immunology microbiology and infectious diseases, 2018
    Co-Authors: Christian Diniz Beduschi Travassos Alves, Renata Da Fontoura Budaszewski, Samuel Paulo Cibulski, M. N. Weber, Fabiana Quoos Mayer, Matheus Viezzer Bianchi, Bruna Zafalon-silva, Guilherme Konradt, Mônica Slaviero, Luciana Sonne
    Abstract:

    Abstract Astroviruses are a common cause of gastroenteritis in children worldwide and can also cause infection in a range of domestic and wild animal species. Canine astrovirus (formally named as Mamastrovirus 5, MAstV5) has been reported worldwide, and its role as an enteric pathogen is still controversial. Herein, we describe the genomic characterization of a MAstV5 (strain crab-eating fox/2016/BRA) identified in a wild canid (Cerdocyon Thous) diagnosed with canine distemper virus (CDV) as causa mortis. The nearly complete genome comprised 6579 nt in length and displayed the archetypal organization of astroviruses. The present report is the first evidence of MAstV5 infection in an animal species other than the dog and highlights a possible natural astrovirus spillover between domestic and wild canids. Moreover, these results show the first evidence of extra-intestinal MAstV5, suggesting a virus systemic spread. This work is expected to contribute to a better understanding of the astroviruses biology and their interactions with the wildlife health.

  • Natural Infection of Wild Canids (Cerdocyon Thous and Lycalopex gymnocercus) with the Intraendothelial Piroplasm Rangelia vitalii in Southern Brazil.
    Journal of wildlife diseases, 2015
    Co-Authors: Gabriela Fredo, Matheus Viezzer Bianchi, Caroline Pinto De Andrade, Suyene Oltramari De Souza, Ronaldo V. Leite-filho, Marcele Bettim Bandinelli, Derek Blaese De Amorim, David Driemeier, Luciana Sonne
    Abstract:

    Rangelia vitalii is a piroplasm that infects canines, causing lesions typical of a hemolytic disorder. Two wild canids, a crab-eating fox (Cerdocyon Thous) and a Pampas fox (Lycalopex gymnocercus), were presented for necropsy in Setor de Patologia Veterinaria at the Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. On gross examination, both animals had pale mucosae and moderate tick infestation (Amblyomma aureolatum). There was severe splenomegaly, and the liver had a diffusely orange-reddish lobular pattern. The mesenteric lymph nodes were brownish and slightly enlarged. Structures compatible with R. vitalii were observed in the cytoplasm of endothelial cells in the liver, stomach, heart, kidney, lungs, lymph nodes, and bladder. The agent was characterized by PCR and genetic sequencing of liver samples and ticks. We show that parasitism with R. vitalii follows an epidemiologic cycle in which wild canids act as reservoirs.

Carlos Henrique Nery Costa - One of the best experts on this subject based on the ideXlab platform.

  • occurrence of lutzomyia longipalpis lutz neiva 1912 and Cerdocyon Thous linnaeus 1977 in a visceral leishmaniasis endemic area in brazil
    Acta Tropica, 2017
    Co-Authors: Maria Regiane Araujo Soares, Ivete Lopes De Mendonca, Jadson Emanuel Lopes Antunes, Rogério Nora Lima, Carlos Henrique Nery Costa
    Abstract:

    Abstract Cerdocyon Thous presents a wide geographic distribution in Brazil and its role as a possible Leishmania infantum reservoir in a visceral leishmaniasis (VL) transmission cycle regardless of dogs ( Canis familiaris ) has been discussed. From this perspective, this work describes the occurrence and use of the habitat by Cerdocyon Thous in a Lutzomyia longipalpis occurrence area Teresina (Piaui − Brazil), VL endemic region. Three specimens of C. Thous were monitored with the use of radio telemetry and trails and footprints, seeking to find possible natural dens in order to collect the sanflies from the site. Luminous CDC and Damasceno traps were simultaneously installed at the visited sites, where two specimens of L. longipalpis and one L. termitophila were captured. The identification of the dens and trails, allows us to infer that the dens are not used only by the C. Thous . Finding the VL vector in natural C. Thous natural dens, reinforces the hypothesis of transmission of Le. infantum in the outskirts of the large urban centers, in a cycle that independs from dogs.

  • Occurrence of Lutzomyia longipalpis Lutz & Neiva 1912 and Cerdocyon Thous Linnaeus 1977, in a visceral leishmaniasis endemic area in Brazil.
    Acta tropica, 2017
    Co-Authors: Maria Regiane Araujo Soares, Ivete Lopes De Mendonca, Jadson Emanuel Lopes Antunes, Rogério Nora Lima, Carlos Henrique Nery Costa
    Abstract:

    Abstract Cerdocyon Thous presents a wide geographic distribution in Brazil and its role as a possible Leishmania infantum reservoir in a visceral leishmaniasis (VL) transmission cycle regardless of dogs ( Canis familiaris ) has been discussed. From this perspective, this work describes the occurrence and use of the habitat by Cerdocyon Thous in a Lutzomyia longipalpis occurrence area Teresina (Piaui − Brazil), VL endemic region. Three specimens of C. Thous were monitored with the use of radio telemetry and trails and footprints, seeking to find possible natural dens in order to collect the sanflies from the site. Luminous CDC and Damasceno traps were simultaneously installed at the visited sites, where two specimens of L. longipalpis and one L. termitophila were captured. The identification of the dens and trails, allows us to infer that the dens are not used only by the C. Thous . Finding the VL vector in natural C. Thous natural dens, reinforces the hypothesis of transmission of Le. infantum in the outskirts of the large urban centers, in a cycle that independs from dogs.

  • antibodies against lutzomyia longipalpis saliva in the fox Cerdocyon Thous and the sylvatic cycle of leishmania chagasi
    Transactions of The Royal Society of Tropical Medicine and Hygiene, 2007
    Co-Authors: Regis Bernardo Brandim Gomes, Ivete Lopes De Mendonca, Vladimir Costa Silva, Jeronimo Lopes Ruas, Mauro R B Silva, Maria Do Socorro Pires E Cruz, Aldina Barral, Carlos Henrique Nery Costa
    Abstract:

    Summary Sera of 11 wild Cerdocyon Thous foxes from an endemic area for American visceral leishmaniasis were tested for the presence of antibodies against salivary gland homogenates (SGH) of Lutzomyia longipalpis . All foxes had higher levels of anti- Lu. longipalpis SGH antibodies than foxes from non-endemic areas, suggesting contact between foxes and the vector of visceral leishmaniasis. Sera of humans and dogs living in the same area were also tested for reactivity against Lu. longipalpis SGHs and had a lower proportion of reactivity than foxes. Antibodies against Leishmania chagasi were not detected in any of the foxes, but three foxes showed the presence of parasites in the bone marrow by direct examination, PCR or by infecting the vector. Both humans and dogs had higher levels of anti- Le. chagasi IgG antibodies than C. Thous . The finding of an antibody response against saliva of Lu. longipalpis among C. Thous together with the broad distribution of the vector in resting areas of infected foxes suggests that the natural foci of transmission of Le. chagasi exists independently of the transmission among dogs and humans.

Helio Langoni - One of the best experts on this subject based on the ideXlab platform.

  • toxoplasma gondii and leishmania spp infection in captive crab eating foxes Cerdocyon Thous carnivora canidae from brazil
    Veterinary Parasitology, 2010
    Co-Authors: Lilian S Catenacci, Juliana Griese, Rodrigo C Da Silva, Helio Langoni
    Abstract:

    The zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. Thus, the present study aimed to assess the prevalence of Leishmania spp. and Toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (Cerdocyon Thous) kept in 17 zoos in Sao Paulo State, Brazil. Modified agglutination test (MAT, for toxoplasmosis) and indirect fluorescent antibody test (IFAT, for toxoplasmosis and leishmaniasis) were employed with heterologous anti-dog immunoglobulin. Antibodies to T. gondii were found in 19.2% animals, with an almost perfect concordance (κ = 0.86; standard error = 9.31%; CI95% = 68.25–104.76%; P < 0.0001) and a strong correlation coefficient (rs = 0.87; P < 0.0001), which allows the use of heterologous anti-dog immunoglobulin to perform IFAT for toxoplasmosis in crab-eating foxes. No sample was positive for Leishmania spp. Toxoplasmosis infection occurs in wild animals from the studied Brazilian zoos, which indicates a probable environmental contamination, highlighting the importance of appropriate zoo management and the action of the parasite as a sentinel to human infection.

  • Toxoplasma gondii and Leishmania spp. infection in captive crab-eating foxes, Cerdocyon Thous (Carnivora, Canidae) from Brazil.
    Veterinary parasitology, 2009
    Co-Authors: Lilian S Catenacci, Juliana Griese, Rodrigo C Da Silva, Helio Langoni
    Abstract:

    The zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. Thus, the present study aimed to assess the prevalence of Leishmania spp. and Toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (Cerdocyon Thous) kept in 17 zoos in São Paulo State, Brazil. Modified agglutination test (MAT, for toxoplasmosis) and indirect fluorescent antibody test (IFAT, for toxoplasmosis and leishmaniasis) were employed with heterologous anti-dog immunoglobulin. Antibodies to T. gondii were found in 19.2% animals, with an almost perfect concordance (kappa=0.86; standard error=9.31%; CI95%=68.25-104.76%; P

Maria Regiane Araujo Soares - One of the best experts on this subject based on the ideXlab platform.

  • occurrence of lutzomyia longipalpis lutz neiva 1912 and Cerdocyon Thous linnaeus 1977 in a visceral leishmaniasis endemic area in brazil
    Acta Tropica, 2017
    Co-Authors: Maria Regiane Araujo Soares, Ivete Lopes De Mendonca, Jadson Emanuel Lopes Antunes, Rogério Nora Lima, Carlos Henrique Nery Costa
    Abstract:

    Abstract Cerdocyon Thous presents a wide geographic distribution in Brazil and its role as a possible Leishmania infantum reservoir in a visceral leishmaniasis (VL) transmission cycle regardless of dogs ( Canis familiaris ) has been discussed. From this perspective, this work describes the occurrence and use of the habitat by Cerdocyon Thous in a Lutzomyia longipalpis occurrence area Teresina (Piaui − Brazil), VL endemic region. Three specimens of C. Thous were monitored with the use of radio telemetry and trails and footprints, seeking to find possible natural dens in order to collect the sanflies from the site. Luminous CDC and Damasceno traps were simultaneously installed at the visited sites, where two specimens of L. longipalpis and one L. termitophila were captured. The identification of the dens and trails, allows us to infer that the dens are not used only by the C. Thous . Finding the VL vector in natural C. Thous natural dens, reinforces the hypothesis of transmission of Le. infantum in the outskirts of the large urban centers, in a cycle that independs from dogs.

  • Occurrence of Lutzomyia longipalpis Lutz & Neiva 1912 and Cerdocyon Thous Linnaeus 1977, in a visceral leishmaniasis endemic area in Brazil.
    Acta tropica, 2017
    Co-Authors: Maria Regiane Araujo Soares, Ivete Lopes De Mendonca, Jadson Emanuel Lopes Antunes, Rogério Nora Lima, Carlos Henrique Nery Costa
    Abstract:

    Abstract Cerdocyon Thous presents a wide geographic distribution in Brazil and its role as a possible Leishmania infantum reservoir in a visceral leishmaniasis (VL) transmission cycle regardless of dogs ( Canis familiaris ) has been discussed. From this perspective, this work describes the occurrence and use of the habitat by Cerdocyon Thous in a Lutzomyia longipalpis occurrence area Teresina (Piaui − Brazil), VL endemic region. Three specimens of C. Thous were monitored with the use of radio telemetry and trails and footprints, seeking to find possible natural dens in order to collect the sanflies from the site. Luminous CDC and Damasceno traps were simultaneously installed at the visited sites, where two specimens of L. longipalpis and one L. termitophila were captured. The identification of the dens and trails, allows us to infer that the dens are not used only by the C. Thous . Finding the VL vector in natural C. Thous natural dens, reinforces the hypothesis of transmission of Le. infantum in the outskirts of the large urban centers, in a cycle that independs from dogs.

Lilian S Catenacci - One of the best experts on this subject based on the ideXlab platform.

  • toxoplasma gondii and leishmania spp infection in captive crab eating foxes Cerdocyon Thous carnivora canidae from brazil
    Veterinary Parasitology, 2010
    Co-Authors: Lilian S Catenacci, Juliana Griese, Rodrigo C Da Silva, Helio Langoni
    Abstract:

    The zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. Thus, the present study aimed to assess the prevalence of Leishmania spp. and Toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (Cerdocyon Thous) kept in 17 zoos in Sao Paulo State, Brazil. Modified agglutination test (MAT, for toxoplasmosis) and indirect fluorescent antibody test (IFAT, for toxoplasmosis and leishmaniasis) were employed with heterologous anti-dog immunoglobulin. Antibodies to T. gondii were found in 19.2% animals, with an almost perfect concordance (κ = 0.86; standard error = 9.31%; CI95% = 68.25–104.76%; P < 0.0001) and a strong correlation coefficient (rs = 0.87; P < 0.0001), which allows the use of heterologous anti-dog immunoglobulin to perform IFAT for toxoplasmosis in crab-eating foxes. No sample was positive for Leishmania spp. Toxoplasmosis infection occurs in wild animals from the studied Brazilian zoos, which indicates a probable environmental contamination, highlighting the importance of appropriate zoo management and the action of the parasite as a sentinel to human infection.

  • Toxoplasma gondii and Leishmania spp. infection in captive crab-eating foxes, Cerdocyon Thous (Carnivora, Canidae) from Brazil.
    Veterinary parasitology, 2009
    Co-Authors: Lilian S Catenacci, Juliana Griese, Rodrigo C Da Silva, Helio Langoni
    Abstract:

    The zoonoses toxoplasmosis and leishmaniasis are important worldwide and also affect wild animals. Thus, the present study aimed to assess the prevalence of Leishmania spp. and Toxoplasma gondii antibodies in 52 serum samples from captive crab-eating foxes (Cerdocyon Thous) kept in 17 zoos in São Paulo State, Brazil. Modified agglutination test (MAT, for toxoplasmosis) and indirect fluorescent antibody test (IFAT, for toxoplasmosis and leishmaniasis) were employed with heterologous anti-dog immunoglobulin. Antibodies to T. gondii were found in 19.2% animals, with an almost perfect concordance (kappa=0.86; standard error=9.31%; CI95%=68.25-104.76%; P