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Wang Hong - One of the best experts on this subject based on the ideXlab platform.
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Changes of the plasmolemma Ca2+-ATPase activities of winter wheat seedlings under 2°C low temperature.
Chinese Journal of Appplied Environmental Biology, 2020Co-Authors: Wang Hong, Jian Ling-chengAbstract:Cerium Trichloride precipitation method was used in electromicroscopic-cytochemical assay to study the localization of the Ca 2+ ATPase activities on plasmolemma of the winter wheat seedlings ( Tritium aestivum L.) under optimal temperature and 2℃ low temperature. The conclusions were obtained as:1) Partial Ca 2+ ATPase activities were observed on the plasmolemma of winter wheat seedlings under normal temperature. 2) Winter wheat seedlings were try 2℃ for 1 h, Ca 2+ ATPase activities were increased on plasmolemma. Further increase in the enzyme activities was obtained after 3 h. After 12 h, Ca 2+ ATPase activities were very strong and the plasmolemma Ca 2+ ATPase activities were the highest after 24 h under 2℃. Plasmolemma Ca 2+ ATPase activities were slightly decreased under 2℃ for 3 d, but still higher than those of control. The cellular ultrastructures were also intacted. The results infer that plasmolemma Ca 2+ ATPase activities were feedback to Ca 2+ intensity level. Fig 1, Ref 10
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The relationship of the plasma membrane Mg 2+ ATPase activity in wheat seedlings to low temperature injury and recovery
Journal of Chinese Electron Microscopy Society, 1999Co-Authors: Wang HongAbstract:In this paper,the changes of ATPase activities in the cells of wheat ( Triticum aestivum L. ) seedlings right after low temperature stress,during the period of recovery,and after the recovery were studied using cytochemical method of Cerium Trichloride precipitates,the main results are summarized as follows: 1). Wheat seedlings grew at the optimum temperature (20℃):The ATPase in young leaves cells of the wheat seedlings mainly concentrated on plasmolemma,cell wall and intercellular spaces.Among these 3 places,the ATPase in plasmolemma manifested the highest activity. 2). After being treated at the temperature of -2℃ for 16 hrs,wheat seedlings were fixed immediately:In comparison with the non treated seedlings,the ATPase activities in plasmolemma significantly decreased or inactivated and the cells′ ultrastructures were damaged. 3). Wheat seedlings were treated at the low temperature of -2℃ for 16 hrs,then followed by a recovery period of 24 hrs:In comparison with the seedlings without such a recovery period,the ATPase activities in plasmolemma recovered in a large degree. 4). Wheat seedlings were treated at the temperature of -2℃ for 16 hrs,then being treated with 100mmol/L CaCl 2 solute,and followed by a recovery period of 24 hrs:In comparison with the seedlings non treated with CaCl 2 solute,the ATPase activities in plasmolemma increased. 5). Wheat seedlings were treated at the temperature of -2℃ for 16 hrs,then treated with 100 mmol/L Na 3VO 4 solute,and followed by a recovery period of 24 hrs:In comparison with the seedlings non treated with Na 3VO 4 solute,the ATPase activities in plasmolemma were lost completely and the cells ultrastructures were severely damaged. 6). Wheat seedlings were treated at the temperature of -2℃ for 16 hrs,then followed by a recovery period of 72 hrs:The ATPase activities in plasmolemma recovered to the normal level and the growth of the seedlings also recovered to the normal level.
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Stability effects of cold-acclimation on the plasmolemma Ca~(2+)-ATPase of winter wheat seedlings
Acta Botanica Sinica, 1998Co-Authors: Wang Hong, Jian Ling-chengAbstract:The cold-acclimation effects on the Ca~(2+)-ATPase activities in plasmolemma of the winter wheat seedlings (Triticum aestivum L.) were studied with electromicroscopic-cytochemical assay in which Cerium Trichloride precipitation method was adopted. The main conclusions are: (1) The plasmolemma Ca~(2+)-ATPase activities of the wheat seedlings treated at -9℃ for 3h decreased considerably as compared with those of the seedlings grown at the optimal temperature of 20℃. A further impediment of the enzyme activities was observed when the cold-stress was prolonged to 12h at -9℃. And complete enzyme inactivation as well as damage of ultrastructure of cells occurred when the seedlings were subjected to cold-stress at -9℃ for 24h. (2) If seedlings were cold-acclimated at 2℃ for 15d, the plasmolemma Ca~(2+)-ATPase activities were higher than those of the non-acclimated seedlings.
Jim-min Fang - One of the best experts on this subject based on the ideXlab platform.
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An Improved Method for the Addition Reactions of 1,3‐Dichloroacetone with Combined Organolithium‐Cerium Trichloride Reagents
Journal of The Chinese Chemical Society, 2020Co-Authors: Same-ting Chen, Jim-min FangAbstract:Alkyl-, phenyl- and alkynyllithium reagents in combination with anhydrous Cerium(III) chloride underwent addition reactions with 1,3-dichloroacetone in a very efficient manner. The addition products are versatile precursors for 2-substituted epichlorohydrins and glycidols. Fluconazole, a potent antifungal agent, was thus synthesized in 67% yield by addition of 1,3-dichloroacetone to 2,4-difluorophenyllithium in the presence of Cerium(III) chloride, followed by substitution of the chlorine atoms with 1,2,4-triazole.
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an improved method for the addition reactions of 1 3 dichloroacetone with combined organolithium Cerium Trichloride reagents
Journal of The Chinese Chemical Society, 2003Co-Authors: Same-ting Chen, Jim-min FangAbstract:Alkyl-, phenyl- and alkynyllithium reagents in combination with anhydrous Cerium(III) chloride underwent addition reactions with 1,3-dichloroacetone in a very efficient manner. The addition products are versatile precursors for 2-substituted epichlorohydrins and glycidols. Fluconazole, a potent antifungal agent, was thus synthesized in 67% yield by addition of 1,3-dichloroacetone to 2,4-difluorophenyllithium in the presence of Cerium(III) chloride, followed by substitution of the chlorine atoms with 1,2,4-triazole.
Jian Ling-cheng - One of the best experts on this subject based on the ideXlab platform.
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Changes of the plasmolemma Ca2+-ATPase activities of winter wheat seedlings under 2°C low temperature.
Chinese Journal of Appplied Environmental Biology, 2020Co-Authors: Wang Hong, Jian Ling-chengAbstract:Cerium Trichloride precipitation method was used in electromicroscopic-cytochemical assay to study the localization of the Ca 2+ ATPase activities on plasmolemma of the winter wheat seedlings ( Tritium aestivum L.) under optimal temperature and 2℃ low temperature. The conclusions were obtained as:1) Partial Ca 2+ ATPase activities were observed on the plasmolemma of winter wheat seedlings under normal temperature. 2) Winter wheat seedlings were try 2℃ for 1 h, Ca 2+ ATPase activities were increased on plasmolemma. Further increase in the enzyme activities was obtained after 3 h. After 12 h, Ca 2+ ATPase activities were very strong and the plasmolemma Ca 2+ ATPase activities were the highest after 24 h under 2℃. Plasmolemma Ca 2+ ATPase activities were slightly decreased under 2℃ for 3 d, but still higher than those of control. The cellular ultrastructures were also intacted. The results infer that plasmolemma Ca 2+ ATPase activities were feedback to Ca 2+ intensity level. Fig 1, Ref 10
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Stability effects of cold-acclimation on the plasmolemma Ca~(2+)-ATPase of winter wheat seedlings
Acta Botanica Sinica, 1998Co-Authors: Wang Hong, Jian Ling-chengAbstract:The cold-acclimation effects on the Ca~(2+)-ATPase activities in plasmolemma of the winter wheat seedlings (Triticum aestivum L.) were studied with electromicroscopic-cytochemical assay in which Cerium Trichloride precipitation method was adopted. The main conclusions are: (1) The plasmolemma Ca~(2+)-ATPase activities of the wheat seedlings treated at -9℃ for 3h decreased considerably as compared with those of the seedlings grown at the optimal temperature of 20℃. A further impediment of the enzyme activities was observed when the cold-stress was prolonged to 12h at -9℃. And complete enzyme inactivation as well as damage of ultrastructure of cells occurred when the seedlings were subjected to cold-stress at -9℃ for 24h. (2) If seedlings were cold-acclimated at 2℃ for 15d, the plasmolemma Ca~(2+)-ATPase activities were higher than those of the non-acclimated seedlings.
Lynn A. Boatner - One of the best experts on this subject based on the ideXlab platform.
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Single-crystal CeCl3(CH3OH)4: A new metal-organic Cerium chloride methanol adduct for scintillator applications
Applied Physics Letters, 2008Co-Authors: Lynn A. Boatner, Bryan C. Chakoumakos, Joanne Oxendine Ramey, James A. Kolopus, John S. Neal, D. Wisniewski, M. Wisniewska, Radu CustelceanAbstract:A new scintillator material consisting of a methanol adduct of Cerium Trichloride with the composition CeCl3(CH3OH)4 has been discovered and crystallized in the form of large single crystals by solution growth in methanol. The peak emission of the x-ray-excited luminescence spectrum occurs at ∼364 nm. A light yield of up to ∼16 600 photons/MeV and an energy resolution of 11.4% were obtained using 662 keV gamma-ray photons. The scintillator decay time for 662 keV gamma-ray excitation was measured using the time-correlated, single-photon-counting method, and a nominal value of 64.4 ns was obtained. The molecular adduct CeCl3(CH3OH)4 represents the first example of a rare-earth, metal-organic scintillator that is applicable to gamma ray, x ray, and neutron detection.
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Cerium Chloride-methanol Adduct Crystals, CeCl3(CH3OH)4: Preparation, Crystallography, And Scintillation Properties
Crystal Growth & Design, 2008Co-Authors: Bryan C. Chakoumakos, Radu Custelcean, Joanne Oxendine Ramey, James A. Kolopus, John S. Neal, D. Wisniewski, Lynn A. BoatnerAbstract:A new methanol adduct of Cerium Trichloride of composition CeCl3(CH3OH)4 was crystallized from a solution growth in methanol. Large single crystals (more than several cubic centimeters) have been grown in controlled temperature baths. The molecular adduct of composition Ce2Cl6(CH3OH)8 is determined from X-ray crystal structure analysis, and this molecule undoubtedly persists in solution as well. Motivations for studying these compounds are their promising unique scintillation properties for radiation detection applications and the model structural systems they provide for the realization of new low-dimensional quantum magnets. This material apparently represents the first example of a metal-organic scintillator that is applicable to X-ray, γ-ray, and neutron detection.
D. Wisniewski - One of the best experts on this subject based on the ideXlab platform.
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A New Scintillator for Fast Neutron Detection: Single-Crystal ${\rm CeCl}_{3}({\rm CH}_{3}{\rm OH})_{4}$
IEEE Transactions on Nuclear Science, 2010Co-Authors: J. S. Neal, D. Wisniewski, L. A. Boatner, Z. W. Bell, S. M. Mcconchie, J. O. Ramey, J. A. Kolopus, B. C. Chakoumakos, M. Wisniewska, R. CustelceanAbstract:A new scintillator material for the detection of fast neutrons that consists of a methanol adduct of Cerium Trichloride with the composition CeCl3(CH3OH)4 has been characterized using 14.1 MeV neutrons from a deuterium-tritium neutron generator and fast neutrons from a bare instrumented 252Cf source. The timing resolution of the scintillator for fast neutrons was found to be 1~ ns. Neutron interactions in the CeCl3(CH3OH)4 composition were simulated using the MCNP-PoliMi code. These simulations indicate that proton recoils account for most of the deposited energy in CeCl3(CH3OH)4. The crystalline molecular adduct CeCl3(CH3OH)4 represents a rare-earth metal-organic scintillator that can be applied to both fast neutron and gamma-ray detection.
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Single-crystal CeCl3(CH3OH)4: A new metal-organic Cerium chloride methanol adduct for scintillator applications
Applied Physics Letters, 2008Co-Authors: Lynn A. Boatner, Bryan C. Chakoumakos, Joanne Oxendine Ramey, James A. Kolopus, John S. Neal, D. Wisniewski, M. Wisniewska, Radu CustelceanAbstract:A new scintillator material consisting of a methanol adduct of Cerium Trichloride with the composition CeCl3(CH3OH)4 has been discovered and crystallized in the form of large single crystals by solution growth in methanol. The peak emission of the x-ray-excited luminescence spectrum occurs at ∼364 nm. A light yield of up to ∼16 600 photons/MeV and an energy resolution of 11.4% were obtained using 662 keV gamma-ray photons. The scintillator decay time for 662 keV gamma-ray excitation was measured using the time-correlated, single-photon-counting method, and a nominal value of 64.4 ns was obtained. The molecular adduct CeCl3(CH3OH)4 represents the first example of a rare-earth, metal-organic scintillator that is applicable to gamma ray, x ray, and neutron detection.
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Cerium Chloride-methanol Adduct Crystals, CeCl3(CH3OH)4: Preparation, Crystallography, And Scintillation Properties
Crystal Growth & Design, 2008Co-Authors: Bryan C. Chakoumakos, Radu Custelcean, Joanne Oxendine Ramey, James A. Kolopus, John S. Neal, D. Wisniewski, Lynn A. BoatnerAbstract:A new methanol adduct of Cerium Trichloride of composition CeCl3(CH3OH)4 was crystallized from a solution growth in methanol. Large single crystals (more than several cubic centimeters) have been grown in controlled temperature baths. The molecular adduct of composition Ce2Cl6(CH3OH)8 is determined from X-ray crystal structure analysis, and this molecule undoubtedly persists in solution as well. Motivations for studying these compounds are their promising unique scintillation properties for radiation detection applications and the model structural systems they provide for the realization of new low-dimensional quantum magnets. This material apparently represents the first example of a metal-organic scintillator that is applicable to X-ray, γ-ray, and neutron detection.