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Robin B Gasser - One of the best experts on this subject based on the ideXlab platform.
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Results of coprodiagnostic testing.
2013Co-Authors: Florian Roeber, Robin B Gasser, John W. A. Larsen, Norman Anderson, Angus J. D. Campbell, Garry A. Anderson, Aaron R. JexAbstract:Results from the testing of 158 individual faecal samples by conventional faecal egg count and species-specific PCRs using the primer pairs HC-NC2 (Haemonchus contortus), TEL-NC2 (Teladorsagia circumcincta), TRI-NC2 (Trichostrongylus spp.), CHO-NC2 (Chabertia ovina) and OEV-NC2 (Oesophagostomum venulosum). Shown are the number of egg count positive samples, mean eggs per gram, standard deviation, and range of strongylid egg counts recorded for the individual groups of sheep. Also shown are the number of species positive samples as determined by PCR for the different groups of sheep on days 0 and 10 of the experiment.aGroups of sheep assigned as ABC (albendazole-untreated control), AB (albendazole-treated), MPC (monepantel-untreated control), MP (monepantel treated).
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Systematic relationships of some members of the genera Oesophagostomum and Chabertia (Nematoda: Chabertiidae) based on ribosomal DNA sequence data.
International Journal for Parasitology, 1998Co-Authors: Lisa A. Newton, Neil B Chilton, Ian Beveridge, Robin B GasserAbstract:Abstract The present study characterised seven species of the Chabertiidae (Nematoda: Strongyloidea) belonging to either the subfamily Oesophagostominae ( Oesophagostomum radiatum, Oesophagostomum venulosum, Oesophagostomum dentatum, Oesophagostomum quadrispinulatum, Oesophagostomum columbianum, Oesophagostomum bifurcum ) or to the subfamily Chabertiinae ( Chabertia ovina ) by their second internal transcribed spacer rDNA sequence, assessed the extent of intraspecific variation and interspecific differences in the sequence, and inferred the phylogenetic relationship of C. ovina with respect to members of the Oesophagostominae. In both the phenetic and cladistic analyses of the sequence data, Chabertia was nested within Oesophagostomum , suggesting either that the species examined represent members of the same genus, or alternatively, that Oesophagostomum may represent more than one genus.
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Phylogenetic relationships of Australian strongyloid nematodes inferred from ribosomal DNA sequence data.
International Journal for Parasitology, 1997Co-Authors: Neil B Chilton, Robin B Gasser, Ian BeveridgeAbstract:The sequence of the second internal transcribed spacer of the ribosomal DNA was determined for the following strongyloid nematodes: Cylicocyclus insignis, Chabertia ovina, Oesophagostomum venulosum, Cloacina communis, Cloacina hydriformis, Labiostrongylus labiostrongylus, Parazoniolaimus collaris, Macropostrongylus macropostrongylus, Macropostrongylus yorkei, Rugopharynx australis, Rugopharynx rosemariae, Macropostrongyloides baylisi, Oesophagostomoides longispicularis and Paramacropostrongylus toraliformis, and compared with published sequences for species of Strongylus and for Hypodontus macropi. The resultant phylogenetic trees supported current hypotheses based on morphological evidence for the separation of the families Strongylidae and Chabertiidae, but did not support the separation of the endemic Australian genera as a distinctive clade within the Chabertiidae. The implications of this finding for the phylogenetic origins of the Australian strongyloids are discussed.
Ian Beveridge - One of the best experts on this subject based on the ideXlab platform.
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Systematic relationships of some members of the genera Oesophagostomum and Chabertia (Nematoda: Chabertiidae) based on ribosomal DNA sequence data.
International Journal for Parasitology, 1998Co-Authors: Lisa A. Newton, Neil B Chilton, Ian Beveridge, Robin B GasserAbstract:Abstract The present study characterised seven species of the Chabertiidae (Nematoda: Strongyloidea) belonging to either the subfamily Oesophagostominae ( Oesophagostomum radiatum, Oesophagostomum venulosum, Oesophagostomum dentatum, Oesophagostomum quadrispinulatum, Oesophagostomum columbianum, Oesophagostomum bifurcum ) or to the subfamily Chabertiinae ( Chabertia ovina ) by their second internal transcribed spacer rDNA sequence, assessed the extent of intraspecific variation and interspecific differences in the sequence, and inferred the phylogenetic relationship of C. ovina with respect to members of the Oesophagostominae. In both the phenetic and cladistic analyses of the sequence data, Chabertia was nested within Oesophagostomum , suggesting either that the species examined represent members of the same genus, or alternatively, that Oesophagostomum may represent more than one genus.
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Phylogenetic relationships of Australian strongyloid nematodes inferred from ribosomal DNA sequence data.
International Journal for Parasitology, 1997Co-Authors: Neil B Chilton, Robin B Gasser, Ian BeveridgeAbstract:The sequence of the second internal transcribed spacer of the ribosomal DNA was determined for the following strongyloid nematodes: Cylicocyclus insignis, Chabertia ovina, Oesophagostomum venulosum, Cloacina communis, Cloacina hydriformis, Labiostrongylus labiostrongylus, Parazoniolaimus collaris, Macropostrongylus macropostrongylus, Macropostrongylus yorkei, Rugopharynx australis, Rugopharynx rosemariae, Macropostrongyloides baylisi, Oesophagostomoides longispicularis and Paramacropostrongylus toraliformis, and compared with published sequences for species of Strongylus and for Hypodontus macropi. The resultant phylogenetic trees supported current hypotheses based on morphological evidence for the separation of the families Strongylidae and Chabertiidae, but did not support the separation of the endemic Australian genera as a distinctive clade within the Chabertiidae. The implications of this finding for the phylogenetic origins of the Australian strongyloids are discussed.
M. Z. Chishti - One of the best experts on this subject based on the ideXlab platform.
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Bionomics of Helminth Parasites in Goats of Subtropical Jammu Area of
2015Co-Authors: Muzaffar Rasool Mir, M. Z. Chishti, S. A. Dar, Majidah RashidAbstract:A survey was carried out on the prevalence of gastrointestinal (GI) helminthes in 284 goats in different areas of subtropical Jammu region of Jammu and Kashmir State. Necroscopic examination revealed 72.88 % of infection. The gastrointestinal helminthes isolated were Haemonchus contortus (28.87%), Trichuris ovis (14.43%), Paramphistomum cervi (6.69%), Fasciola hepatica (5.28%), and Chabertia ovine (4.57%,) Dicrocillium dendriticum (3.87%), Dictyocaulus filarae (3.52%), Stilesia globipunctata (3.52%) and Monezia expansa (2.11%) The seasonal distribution of infection indicated a higher percentage of infection in summer and winter followed by spring and autumn
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Bionomics of Helminth Parasites in Goats of Subtropical Jammu Area of J&K, India
Agriculture and Biology Journal of North America, 2013Co-Authors: Muzaffar Mir, M. Z. ChishtiAbstract:A survey was carried out on the prevalence of gastrointestinal (GI) helminthes in 284 goats in different areas of subtropical Jammu region of Jammu and Kashmir State. Necroscopic examination revealed 72.88% of infection. The gastrointestinal helminthes isolated were Haemonchus contortus (28.87%), Trichuris ovis (14.43%), Paramphistomum cervi (6.69%), Fasciola hepatica (5.28%), and Chabertia ovine (4.57%,) Dicrocillium dendriticum (3.87%), Dictyocaulus filarae (3.52%), Stilesia globipunctata (3.52%) and Monezia expansa (2.11%) The seasonal distribution of infection indicated a higher percentage of infection in summer and winter followed by spring and autumn.
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RESEARCH ARTICLE Prevalence of gastrointestinal nematodes in goats of jammu region
2013Co-Authors: Muzaffar Rasool Mir, M. Z. Chishti, Majidah Rashid, Shabir Ahmad Dar, Javaid Kuchay, Javid Ahmad DarAbstract:The seasonal prevalence of gastrointestinal nematodes in goat of Jammu region was carried out between June 2007 to July 2008 through faecal examination. Nematode infections were detected in 72. 5% of 680 faecal samples collected from the host in different areas of Jammu including Bisnah, R S Pora, Akhnoor, Negrota and Sidrah. Haemonchous, Ostertagia, Chabertia, Bunostomum, Trichostrongylus, Nematodirus, Oesophagostomum, Marshallagia, Trichuris etc. were nematodes whose eggs were detected in the faecal samples. The Haemonchous and Ostertagia genera were the predominant among all the genera. Higher infection coincided with the raining season in the spring and summer months then progressively declined during the dry season. The gastrointestinal nematode infection was more prevalent in zero to one year age group. Mature animals remain substantially resistant to serious infection, although maintenance of immunity requires regular exposure to parasites.
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Short Communication Prevalence of nematode parasites in Sheep of
2011Co-Authors: Ladakh India, M. Z. Chishti, S. A. Dar, J. A. Kuchai, Manal M. Zaki, Javid Ahmad, Muzaffar Rasool, Hidayatullah TakAbstract:Nematode parasite infections of sheep are a major problem throughout the world and are responsible for economic losses in a variety of ways. In the present study an attempt was made to find out the various nematode parasites and their prevalence infesting the sheep of Ladakh region of Jammu and Kashmir State in India. A total of 313 sheep of either sex and of different age groups belonging to different areas of Ladakh were examined for nematode parasites, out of these 136 (43.45%) were found infected. The study reveals the presence of four species of nematodes viz; Trichuris ovis, Haemonchus contortus, Dictyocaulus filaria and Chabertia ovina. It was also observed that among these T. ovis was most dominant followed by H. contortus, D. filaria and C. ovina, respectively. Furthermore season, sex, age and agro-ecology show an association with prevalence
Neil B Chilton - One of the best experts on this subject based on the ideXlab platform.
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Systematic relationships of some members of the genera Oesophagostomum and Chabertia (Nematoda: Chabertiidae) based on ribosomal DNA sequence data.
International Journal for Parasitology, 1998Co-Authors: Lisa A. Newton, Neil B Chilton, Ian Beveridge, Robin B GasserAbstract:Abstract The present study characterised seven species of the Chabertiidae (Nematoda: Strongyloidea) belonging to either the subfamily Oesophagostominae ( Oesophagostomum radiatum, Oesophagostomum venulosum, Oesophagostomum dentatum, Oesophagostomum quadrispinulatum, Oesophagostomum columbianum, Oesophagostomum bifurcum ) or to the subfamily Chabertiinae ( Chabertia ovina ) by their second internal transcribed spacer rDNA sequence, assessed the extent of intraspecific variation and interspecific differences in the sequence, and inferred the phylogenetic relationship of C. ovina with respect to members of the Oesophagostominae. In both the phenetic and cladistic analyses of the sequence data, Chabertia was nested within Oesophagostomum , suggesting either that the species examined represent members of the same genus, or alternatively, that Oesophagostomum may represent more than one genus.
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Phylogenetic relationships of Australian strongyloid nematodes inferred from ribosomal DNA sequence data.
International Journal for Parasitology, 1997Co-Authors: Neil B Chilton, Robin B Gasser, Ian BeveridgeAbstract:The sequence of the second internal transcribed spacer of the ribosomal DNA was determined for the following strongyloid nematodes: Cylicocyclus insignis, Chabertia ovina, Oesophagostomum venulosum, Cloacina communis, Cloacina hydriformis, Labiostrongylus labiostrongylus, Parazoniolaimus collaris, Macropostrongylus macropostrongylus, Macropostrongylus yorkei, Rugopharynx australis, Rugopharynx rosemariae, Macropostrongyloides baylisi, Oesophagostomoides longispicularis and Paramacropostrongylus toraliformis, and compared with published sequences for species of Strongylus and for Hypodontus macropi. The resultant phylogenetic trees supported current hypotheses based on morphological evidence for the separation of the families Strongylidae and Chabertiidae, but did not support the separation of the endemic Australian genera as a distinctive clade within the Chabertiidae. The implications of this finding for the phylogenetic origins of the Australian strongyloids are discussed.
Xing-quan Zhu - One of the best experts on this subject based on the ideXlab platform.
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Chabertia erschowi (Nematoda) is a distinct species based on nuclear ribosomal DNA sequences and mitochondrial DNA sequences
Parasites & Vectors, 2014Co-Authors: Guo-hua Liu, Lei Zhao, Hui-qun Song, Guang-hui Zhao, Jin-zhong Cai, Quan Zhao, Xing-quan ZhuAbstract:Background: Gastrointestinal nematodes of livestock have major socio-economic importance worldwide. In small ruminants, Chabertia spp. are responsible for economic losses to the livestock industries globally. Although much attention has given us insights into epidemiology, diagnosis, treatment and control of this parasite, over the years, only one species (C. ovina) has been accepted to infect small ruminants, and it is not clear whether C. erschowi is valid as a separate species. Methods: The first and second internal transcribed spacers (ITS-1 and ITS-2) regions of nuclear ribosomal DNA (rDNA) and the complete mitochondrial (mt) genomes of C. ovina and C. erschowi were amplified and then sequenced. Phylogenetic re-construction of 15 Strongylida species (including C. erschowi) was carried out using Bayesian inference (BI) based on concatenated amino acid sequence datasets. Results: The ITS rDNA sequences of C. ovina China isolates and C. erschowi samples were 852–854 bp and 862 -866 bp in length, respectively. The mt genome sequence of C. erschowi was 13,705 bp in length, which is 12 bp shorter than that of C. ovina China isolate. The sequence difference between the entire mt genome of C. ovina China isolate and that of C. erschowi was 15.33%. In addition, sequence comparison of the most conserved mt small subunit ribosomal (rrnS) and the least conserved nad2 genes among multiple individual nematodes revealed substantial nucleotide differences between these two species but limited sequence variation within each species. Conclusions: The mtDNA and rDNA datasets provide robust genetic evidence that C. erschowi is a valid strongylid nematode species. The mtDNA and rDNA datasets presented in the present study provide useful novel markers for further studies of the taxonomy and systematics of the Chabertia species from different hosts and geographical regions.
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Genetic differences between Chabertia ovina and C. erschowi revealed by sequence analysis of four mitochondrial genes.
Mitochondrial DNA, 2013Co-Authors: Lei Zhao, Guo-hua Liu, Guang-hui Zhao, Jin-zhong Cai, Xing-quan Zhu, Ai-dong QianAbstract:AbstractThis study examined sequence differences in mitochondrial cytochrome c oxidase subunit 1 (cox1), large subunit ribosomal RNA (rrnL) and NADH dehydrogenase subunits 1 and 4 (nad1 and nad4) between Chabertia ovina and C. erschowi from yaks in Qinghai and goats in Shaanxi provinces, China. A part of the cox1 (pcox1), rrnL (prrnL), nad1 and nad4 genes (pnad1 and pnad4) were amplified separately from individual nematodes by PCR and sequenced. The length of the sequences of pcox1, prrnL, pnad1 and pnad4 was 441 bp, 450 bp, 526 bp and 914 bp for C. ovina, and 441 bp, 451 bp, 517 bp and 810 bp for C. erschowi, respectively. The intra-specific sequence variations within C. ovina were 0.2–2.9% for pcox1, 0–0.9% for prrnL, 0.6–2.3% for pnad1, and 0.4–2.0% for pnad4, and were 0.5–1.6% for pcox1, 0–1.1% for prrnL, 0.2–1.7% for pnad1, and 0.4–1.1% for pnad4 within C. erschowi. Whereas, the inter-specific sequence differences between the two species were obviously higher, being 11.6–12.9% for pcox1, 9.8–11.1% fo...