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Jason P Londo - One of the best experts on this subject based on the ideXlab platform.
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deconstructing cold hardiness variation in supercooling ability and Chilling Requirements in the wild grapevine vitis riparia
Australian Journal of Grape and Wine Research, 2019Co-Authors: Jason P Londo, Alisson P KovaleskiAbstract:Background and Aims Grapevine production in cool climates is limited by aspects of winter survival and frost risk. Cold hardiness‐related traits are key to future viticultural sustainability as climate variations, including acute cold events and frost, are predicted to increase even in traditional cultivation regions. This study examines the variation in dormant bud cold hardiness (supercooling) and dormancy (Chilling Requirement) in 43 different genotypes of the wild grapevine species Vitis riparia, the dominant wild species used to incorporate cold hardiness traits into new hybrid grapevine cultivars. Methods and Results Cold hardiness was evaluated bi‐weekly in 2 years using measures of low temperature exotherms. Whole winter responses were modelled to determine significant factors affecting cold hardiness and determine genotypic differences. Results demonstrate significant differences in supercooling ability and deacclimation rate (loss of cold hardiness) between genotypes. Conclusions This study determined that genotypic differences contribute to initial differences in cold hardiness. However, data modelling suggests that midwinter cold hardiness changes are driven by environment as all Vitis riparia tested in this study respond to temperature in the same manner during the endodormant period of winter. In contrast, responses to warming temperature during ecodormancy are significantly different by genotype. Significance of the Study This study has demonstrated that these two traits interact to determine differences in early versus late winter cold hardiness and help identify breeding germplasm with delayed loss of cold hardiness.
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deacclimation kinetics as a quantitative phenotype for delineating the dormancy transition and thermal efficiency for budbreak in vitis species
Aob Plants, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species. In grapevine, acquisition of cold hardiness requires dormancy induction in the early winter and careful maintenance of dormancy state throughout winter. With sufficient exposure to low, non-freezing temperatures (Chilling Requirement), grapevine buds transition between early (endodormant) and late winter (ecodormant) states. The objective of this study was to uncover the relationship between fulfilment of the Chilling Requirement and the effects of various temperatures on loss of cold hardiness (deacclimation). The relationship between Chilling Requirement and temperature as it affects the rate of deacclimation (kdeacc) was examined for dormant cuttings of Vitis vinifera, V. aestivalis, V. amurensis and V. riparia. The effect of temperature on kdeacc was exponential at low and logarithmic at high temperatures. Deacclimation rates also increased in magnitude as Chilling accumulated demonstrating a change in deacclimation potential (Ψdeacc), following a logarithmic response. The combination of Ψdeacc and kdeacc indicates genotype-specific thermal efficiency for deacclimation and growth in Vitis that may be overlooked by simple growing degree-day computations. The Ψdeacc and kdeacc parameters are genotype-specific and will greatly increase the refinement of models predicting effects of climate change on phenology. Deacclimation rates represent a quantitative determinant of dormancy transition and budbreak in grapevine and will assist researchers in selecting germplasm for differences in Chilling Requirement and thermal efficiency.
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kinetics of winter deacclimation in response to temperature determines dormancy status and explains budbreak in different vitis species
bioRxiv, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species, with shifting temperature-based responses during the winter. The objective of this study was to uncover the relationship between dormancy transition (Chilling Requirement) and temperature on the loss of cold hardiness and budbreak. Dormant cuttings of Vitis vinifera , V. aestivalis , V. amurensis , and V. riparia were examined to determine the relationship between Chilling Requirement and temperature on rate of deacclimation ( k deacc ). Differential thermal analysis was used to determine k deacc using mean low temperature exotherms. Effect of chill was evaluated as the deacclimation potential (Ψ deacc ), which was the change in k deacc due to chill accumulation. Budbreak was also evaluated in fully chilled buds at different temperatures. Results indicate that Ψ deacc varies dependent on dormancy state, following a logarithmic response to chill accumulation. The effect of temperature on k deacc was exponential at low and logarithmic at high temperatures. The combination of Ψ deacc and k deacc resulted in good prediction of deacclimation. Budbreak phenology was also explained by differences in k deacc . Deacclimation rates can be used as a quantitative determinant of dormancy transition and budbreak, and to refine models predicting effects of climate change.
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kinetics of winter deacclimation in response to temperature determines dormancy status in grapevines vitis spp
bioRxiv, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Abstract Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species, with shifting temperature-based responses during the winter. The objective of this study was to uncover the relationship between dormancy transition (Chilling Requirement) and temperature on the loss of cold hardiness. Dormant cuttings of European grapevine cultivars (Vitis vinifera; ‘Cabernet Franc’, ‘Cabernet Sauvignon’, ‘Riesling’, and ‘Sauvignon blanc’) and wild grapevine species (V. aestivalis, V. amurensis, and V. riparia) were examined to determine the relationship between Chilling Requirement and temperature on rate of deacclimation (kdeacc). Differential thermal analysis was used to determine kdeacc using mean low temperature exotherms. Effect of chill was evaluated as the deacclimation potential (Ψdeacc), which was the change in kdeacc due to chill accumulation. Results indicate that Ψdeacc varies dependent on dormancy state, following a logarithmic response to chill accumulation. The effect of temperature on kdeacc was exponential at low and logarithmic at high temperatures. The combination of Ψdeacc and kdeacc resulted in good prediction of deacclimation, demonstrating that kdeacc can be used as a quantitative determinant of dormancy transition. This information can be used to refine models predicting effects of climate change on dormancy and cold hardiness in grapevine. Highlight Deacclimation rates in Vitis species appear to follow enzyme kinetics; and using deacclimation rates, dormancy transitions can be studied in a quantitative manner. Abbreviations DTA: Differential thermal analysis HTE: High temperature exotherm LTE: Low temperature exotherm kdeacc: Deacclimation rate Ψdeacc: Deacclimation potential Chill: Chill accumulation t: time T: temperature
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kinetics of winter deacclimation in response to temperature determines dormancy status and quantifies Chilling Requirement in grapevines vitis spp
bioRxiv, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species, with shifting temperature-based responses during the winter. The objective of this study was to uncover the relationship between dormancy transition (Chilling Requirement) and temperature on the loss of cold hardiness. Dormant cuttings of European grapevine cultivars ( Vitis vinifera ; 9Cabernet Franc9, 9Cabernet Sauvignon9, 9Riesling9, and 9Sauvignon blanc9) and wild grapevine species ( V. aestivalis , V. amurensis , and V. riparia ) were examined to determine the relationship between Chilling Requirement and temperature on rate of deacclimation ( k deacc ). Differential thermal analysis was used to determine k deacc using mean low temperature exotherms. Effect of chill was evaluated as the deacclimation potential (Ψ deacc ), which was the change in k deacc due to chill accumulation. Results indicate that Ψ deacc varies dependent on dormancy state, following a logarithmic response to chill accumulation. The effect of temperature on kdeacc was exponential at low and logarithmic at high temperatures. The combination of Ψ deacc and kdeacc resulted in good prediction of deacclimation, demonstrating that k deacc can be used as a quantitative determinant of dormancy transition. This information can be used to refine models predicting effects of climate change on dormancy and cold hardiness in grapevine.
Douglas G Bielenberg - One of the best experts on this subject based on the ideXlab platform.
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Genotyping by Sequencing for SNP-Based Linkage Map Construction and QTL Analysis of Chilling Requirement and Bloom Date in Peach [Prunus persica (L.) Batsch]
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted breeding efforts, but have not been available for many ‘specialty crops’ such as fruit and nut trees. Here we apply the Genotyping-By-Sequencing (GBS) method developed for cereals to the discovery of single nucleotide polymorphisms (SNPs) in a peach F2 mapping population. Peach is a genetic and genomic model within the Rosaceae and will provide a template for the use of this method with other members of this family. Our F2 mapping population of 57 genotypes segregates for bloom time (BD) and Chilling Requirement (CR) and we have extensively phenotyped this population. The population derives from a selfed F1 progeny of a cross between ‘Hakuho’ (high CR) and ‘UFGold’ (low CR). We were able to successfully employ GBS and the TASSEL GBS pipeline without modification of the original methodology using the ApeKI restriction enzyme and multiplexing at an equivalent of 96 samples per Illumina HiSeq 2000 lane. We obtained hundreds of SNP markers which were then used to construct a genetic linkage map and identify quantitative trait loci (QTL) for BD and CR.
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Chilling Requirement QTL detected in 2008/2009 and 2009/2010.
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:A QTL is named as qXXYa—ZZZZ, with ‘XX’ being the trait abbreviation, ‘Y’ the number of the linkage group, ‘a’ the letter to specify different QTLs for the same trait in one linkage group (G), and ‘ZZZZ’ the year in which the trait was phenotyped.Chilling Requirement QTL detected in 2008/2009 and 2009/2010.
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Spearman’s rank order correlation coefficients for observed Chilling Requirement (CR) and bloom date (BD).
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:Coefficients are all significant at the p
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Linkage map of F2 mapping population segregating for Chilling Requirement (CR) and bloom date (BD).
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:Linkage group numbering corresponds to the peach reference genetic map. Left side of bars are marker distances in cM. Labels on the right side of bars are the marker name. Markers with names followed by an asterix have significantly distorted genotypic ratios (*, p
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mapping quantitative trait loci associated with Chilling Requirement heat Requirement and bloom date in peach prunus persica
New Phytologist, 2010Co-Authors: Douglas G Bielenberg, Tetyana Zhebentyayeva, Gregory L. Reighard, William R. Okie, Doron Holland, A G AbbottAbstract:Summary •Chilling Requirement, together with heat Requirement, determines the bloom date, which has an impact on the climatic distribution of the genotypes of tree species. The molecular basis of floral bud Chilling Requirement is poorly understood, despite its importance to the adaptation and production of fruit trees. In addition, the genetic nature of heat Requirement and the genetic interrelationships among Chilling Requirement, heat Requirement and bloom date remain unclear. •A peach (Prunus persica) F2 population of 378 genotypes developed from two genotypes with contrasting Chilling Requirements was used for linkage map construction and quantitative trait loci (QTL) mapping. The floral bud Chilling and heat Requirements of each genotype were evaluated over 2 yr and the bloom date was scored over 4 yr. •Twenty QTLs with additive effects were identified for three traits, including one major QTL for Chilling Requirement and two major QTLs for bloom date. The majority of QTLs colocalized with QTLs for other trait(s). In particular, one genomic region of 2 cM, pleiotropic for the three traits, overlapped with the sequenced peach EVG region. •This first report on the QTL mapping of floral bud Chilling Requirement will facilitate marker-assisted breeding for low Chilling Requirement cultivars and the map-based cloning of genes controlling Chilling Requirement. The extensive colocalization of QTLs suggests that there may be one unified temperature sensing and action system regulating Chilling Requirement, heat Requirement and bloom date together
A Wunsch - One of the best experts on this subject based on the ideXlab platform.
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high density linkage maps constructed in sweet cherry prunus avium l using cross and self pollination populations reveal chromosomal homozygosity in inbred families and non syntenic regions with the peach genome
Tree Genetics & Genomes, 2018Co-Authors: Alejandro Calle, Lichun Cai, Amy F Iezzoni, A WunschAbstract:The landrace sweet cherry (Prunus avium L.) cultivar ‘Cristobalina’ is a useful resource for sweet cherry breeding due to several important traits, including low Chilling Requirement, early maturity date, and self-compatibility. In this work, three families (N = 325), derived from ‘Cristobalina’, were used to develop high-density genetic maps using the RosBREED 6K Illumina Infinium® cherry SNP array. Two of the families were derived from self-pollination, which allowed construction of the first F2 genetic maps in the species. The other map developed was from an interspecific cross of cultivars ‘Vic’ × ‘Cristobalina’. The maps developed include 511 to 816 mapped SNPs covering 622.4 to 726.0 cM. Mapped SNP marker order and position were compared to the sweet cherry and peach genome sequences, and a high degree of synteny was observed. However, inverted and small translocated regions between peach and sweet cherry genomes were observed with the most noticeable inversion at the top of LG5. The progeny resulting from self-pollination also revealed a high level of homozygosity, as large presumably homozygous regions as well as entire homozygous LGs were observed. These maps will be used for genetic analysis of relevant traits in sweet cherry breeding by QTL analysis, and self-pollination populations will be useful for investigating inbreeding depression in a naturally outbreeding species.
Gregory L. Reighard - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Genotyping by Sequencing for SNP-Based Linkage Map Construction and QTL Analysis of Chilling Requirement and Bloom Date in
2016Co-Authors: Glenn Abbott, Gregory L. Reighard, William R. Okie, Christina Elizabeth WellsAbstract:Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted breeding efforts, but have not been available for many ‘specialty crops ’ such as fruit and nut trees. Here we apply the Genotyping-By-Sequencing (GBS) method developed for cereals to the discovery of single nucleotide polymorphisms (SNPs) in a peach F2 map-ping population. Peach is a genetic and genomic model within the Rosaceae and will pro-vide a template for the use of this method with other members of this family. Our F2 mapping population of 57 genotypes segregates for bloom time (BD) and Chilling Requirement (CR) and we have extensively phenotyped this population. The population derives from a selfed F1 progeny of a cross between ‘Hakuho ’ (high CR) and ‘UFGold ’ (low CR). We were able to successfully employ GBS and the TASSEL GBS pipeline without modification of the original methodology using the ApeKI restriction enzyme and multiplexing at an equivalent of 96 samples per Illumina HiSeq 2000 lane. We obtained hundreds of SNP markers which were then used to construct a genetic linkage map and identify quantitative trait loci (QTL) for BD and CR
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Genotyping by Sequencing for SNP-Based Linkage Map Construction and QTL Analysis of Chilling Requirement and Bloom Date in Peach [Prunus persica (L.) Batsch]
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted breeding efforts, but have not been available for many ‘specialty crops’ such as fruit and nut trees. Here we apply the Genotyping-By-Sequencing (GBS) method developed for cereals to the discovery of single nucleotide polymorphisms (SNPs) in a peach F2 mapping population. Peach is a genetic and genomic model within the Rosaceae and will provide a template for the use of this method with other members of this family. Our F2 mapping population of 57 genotypes segregates for bloom time (BD) and Chilling Requirement (CR) and we have extensively phenotyped this population. The population derives from a selfed F1 progeny of a cross between ‘Hakuho’ (high CR) and ‘UFGold’ (low CR). We were able to successfully employ GBS and the TASSEL GBS pipeline without modification of the original methodology using the ApeKI restriction enzyme and multiplexing at an equivalent of 96 samples per Illumina HiSeq 2000 lane. We obtained hundreds of SNP markers which were then used to construct a genetic linkage map and identify quantitative trait loci (QTL) for BD and CR.
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Chilling Requirement QTL detected in 2008/2009 and 2009/2010.
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:A QTL is named as qXXYa—ZZZZ, with ‘XX’ being the trait abbreviation, ‘Y’ the number of the linkage group, ‘a’ the letter to specify different QTLs for the same trait in one linkage group (G), and ‘ZZZZ’ the year in which the trait was phenotyped.Chilling Requirement QTL detected in 2008/2009 and 2009/2010.
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Spearman’s rank order correlation coefficients for observed Chilling Requirement (CR) and bloom date (BD).
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:Coefficients are all significant at the p
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Linkage map of F2 mapping population segregating for Chilling Requirement (CR) and bloom date (BD).
2015Co-Authors: Douglas G Bielenberg, Gregory L. Reighard, William R. Okie, Bradley Rauh, Shenghua Fan, Ksenija Gasic, Albert Glenn Abbott, Christina Elizabeth WellsAbstract:Linkage group numbering corresponds to the peach reference genetic map. Left side of bars are marker distances in cM. Labels on the right side of bars are the marker name. Markers with names followed by an asterix have significantly distorted genotypic ratios (*, p
Alisson P Kovaleski - One of the best experts on this subject based on the ideXlab platform.
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deconstructing cold hardiness variation in supercooling ability and Chilling Requirements in the wild grapevine vitis riparia
Australian Journal of Grape and Wine Research, 2019Co-Authors: Jason P Londo, Alisson P KovaleskiAbstract:Background and Aims Grapevine production in cool climates is limited by aspects of winter survival and frost risk. Cold hardiness‐related traits are key to future viticultural sustainability as climate variations, including acute cold events and frost, are predicted to increase even in traditional cultivation regions. This study examines the variation in dormant bud cold hardiness (supercooling) and dormancy (Chilling Requirement) in 43 different genotypes of the wild grapevine species Vitis riparia, the dominant wild species used to incorporate cold hardiness traits into new hybrid grapevine cultivars. Methods and Results Cold hardiness was evaluated bi‐weekly in 2 years using measures of low temperature exotherms. Whole winter responses were modelled to determine significant factors affecting cold hardiness and determine genotypic differences. Results demonstrate significant differences in supercooling ability and deacclimation rate (loss of cold hardiness) between genotypes. Conclusions This study determined that genotypic differences contribute to initial differences in cold hardiness. However, data modelling suggests that midwinter cold hardiness changes are driven by environment as all Vitis riparia tested in this study respond to temperature in the same manner during the endodormant period of winter. In contrast, responses to warming temperature during ecodormancy are significantly different by genotype. Significance of the Study This study has demonstrated that these two traits interact to determine differences in early versus late winter cold hardiness and help identify breeding germplasm with delayed loss of cold hardiness.
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deacclimation kinetics as a quantitative phenotype for delineating the dormancy transition and thermal efficiency for budbreak in vitis species
Aob Plants, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species. In grapevine, acquisition of cold hardiness requires dormancy induction in the early winter and careful maintenance of dormancy state throughout winter. With sufficient exposure to low, non-freezing temperatures (Chilling Requirement), grapevine buds transition between early (endodormant) and late winter (ecodormant) states. The objective of this study was to uncover the relationship between fulfilment of the Chilling Requirement and the effects of various temperatures on loss of cold hardiness (deacclimation). The relationship between Chilling Requirement and temperature as it affects the rate of deacclimation (kdeacc) was examined for dormant cuttings of Vitis vinifera, V. aestivalis, V. amurensis and V. riparia. The effect of temperature on kdeacc was exponential at low and logarithmic at high temperatures. Deacclimation rates also increased in magnitude as Chilling accumulated demonstrating a change in deacclimation potential (Ψdeacc), following a logarithmic response. The combination of Ψdeacc and kdeacc indicates genotype-specific thermal efficiency for deacclimation and growth in Vitis that may be overlooked by simple growing degree-day computations. The Ψdeacc and kdeacc parameters are genotype-specific and will greatly increase the refinement of models predicting effects of climate change on phenology. Deacclimation rates represent a quantitative determinant of dormancy transition and budbreak in grapevine and will assist researchers in selecting germplasm for differences in Chilling Requirement and thermal efficiency.
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kinetics of winter deacclimation in response to temperature determines dormancy status and explains budbreak in different vitis species
bioRxiv, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species, with shifting temperature-based responses during the winter. The objective of this study was to uncover the relationship between dormancy transition (Chilling Requirement) and temperature on the loss of cold hardiness and budbreak. Dormant cuttings of Vitis vinifera , V. aestivalis , V. amurensis , and V. riparia were examined to determine the relationship between Chilling Requirement and temperature on rate of deacclimation ( k deacc ). Differential thermal analysis was used to determine k deacc using mean low temperature exotherms. Effect of chill was evaluated as the deacclimation potential (Ψ deacc ), which was the change in k deacc due to chill accumulation. Budbreak was also evaluated in fully chilled buds at different temperatures. Results indicate that Ψ deacc varies dependent on dormancy state, following a logarithmic response to chill accumulation. The effect of temperature on k deacc was exponential at low and logarithmic at high temperatures. The combination of Ψ deacc and k deacc resulted in good prediction of deacclimation. Budbreak phenology was also explained by differences in k deacc . Deacclimation rates can be used as a quantitative determinant of dormancy transition and budbreak, and to refine models predicting effects of climate change.
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kinetics of winter deacclimation in response to temperature determines dormancy status in grapevines vitis spp
bioRxiv, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Abstract Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species, with shifting temperature-based responses during the winter. The objective of this study was to uncover the relationship between dormancy transition (Chilling Requirement) and temperature on the loss of cold hardiness. Dormant cuttings of European grapevine cultivars (Vitis vinifera; ‘Cabernet Franc’, ‘Cabernet Sauvignon’, ‘Riesling’, and ‘Sauvignon blanc’) and wild grapevine species (V. aestivalis, V. amurensis, and V. riparia) were examined to determine the relationship between Chilling Requirement and temperature on rate of deacclimation (kdeacc). Differential thermal analysis was used to determine kdeacc using mean low temperature exotherms. Effect of chill was evaluated as the deacclimation potential (Ψdeacc), which was the change in kdeacc due to chill accumulation. Results indicate that Ψdeacc varies dependent on dormancy state, following a logarithmic response to chill accumulation. The effect of temperature on kdeacc was exponential at low and logarithmic at high temperatures. The combination of Ψdeacc and kdeacc resulted in good prediction of deacclimation, demonstrating that kdeacc can be used as a quantitative determinant of dormancy transition. This information can be used to refine models predicting effects of climate change on dormancy and cold hardiness in grapevine. Highlight Deacclimation rates in Vitis species appear to follow enzyme kinetics; and using deacclimation rates, dormancy transitions can be studied in a quantitative manner. Abbreviations DTA: Differential thermal analysis HTE: High temperature exotherm LTE: Low temperature exotherm kdeacc: Deacclimation rate Ψdeacc: Deacclimation potential Chill: Chill accumulation t: time T: temperature
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kinetics of winter deacclimation in response to temperature determines dormancy status and quantifies Chilling Requirement in grapevines vitis spp
bioRxiv, 2018Co-Authors: Alisson P Kovaleski, Bruce I Reisch, Jason P LondoAbstract:Bud dormancy and cold hardiness are critical adaptations for surviving winter cold stress for temperate perennial plant species, with shifting temperature-based responses during the winter. The objective of this study was to uncover the relationship between dormancy transition (Chilling Requirement) and temperature on the loss of cold hardiness. Dormant cuttings of European grapevine cultivars ( Vitis vinifera ; 9Cabernet Franc9, 9Cabernet Sauvignon9, 9Riesling9, and 9Sauvignon blanc9) and wild grapevine species ( V. aestivalis , V. amurensis , and V. riparia ) were examined to determine the relationship between Chilling Requirement and temperature on rate of deacclimation ( k deacc ). Differential thermal analysis was used to determine k deacc using mean low temperature exotherms. Effect of chill was evaluated as the deacclimation potential (Ψ deacc ), which was the change in k deacc due to chill accumulation. Results indicate that Ψ deacc varies dependent on dormancy state, following a logarithmic response to chill accumulation. The effect of temperature on kdeacc was exponential at low and logarithmic at high temperatures. The combination of Ψ deacc and kdeacc resulted in good prediction of deacclimation, demonstrating that k deacc can be used as a quantitative determinant of dormancy transition. This information can be used to refine models predicting effects of climate change on dormancy and cold hardiness in grapevine.