The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Fei Li - One of the best experts on this subject based on the ideXlab platform.
-
multiple mirnas jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects Chilo suppressalis
RNA, 2017Co-Authors: Kang He, Huamei Xiao, Chang Ge, Fei LiAbstract:: The accurate rise and fall of active hormones is important for insect development. The ecdysteroids must be cleared in a timely manner. However, the mechanism of suppressing the ecdysteroid biosynthesis at the right time remains unclear. Here, we sequenced a small RNA library of Chilo suppressalis and identified 300 miRNAs in this notorious rice insect pest. Microarray analysis yielded 54 differentially expressed miRNAs during metamorphosis development. Target prediction and in vitro dual-luciferase assays confirmed that seven miRNAs (two conserved and five novel miRNAs) jointly targeted three Halloween genes in the ecdysteroid biosynthesis pathway. Overexpression of these seven miRNAs reduced the titer of 20-hydroxyecdysone (20E), induced mortality, and retarded development, which could be rescued by treatment with 20E. Comparative analysis indicated that the miRNA regulation of metamorphosis development is a conserved process but that the miRNAs involved are highly divergent. In all, we present evidence that both conserved and lineage-specific miRNAs have crucial roles in regulating development in insects by controlling ecdysteroid biosynthesis, which is important for ensuring developmental convergence and evolutionary diversity.
-
large scale identification of differentially expressed genes during pupa development reveals solute carrier gene is essential for pupal pigmentation in Chilo suppressalis
Journal of Insect Physiology, 2017Co-Authors: Shuijin Huang, Fei Li, Huamei Xiao, Chang Ge, Shuping Wang, Qiupu Yang, Xiaolu TengAbstract:Abstract Insects undergo metamorphosis, involving an abrupt change in body structure through cell growth and differentiation. Rice stem stripped borer (SSB), Chilo suppressalis, is one of the most destructive rice pests. However, little is known about the regulation mechanism of metamorphosis development in this notorious insect pest. Here, we studied the expression of 22,197 SSB genes at seven time points during pupa development with a customized microarray, identifying 622 differentially expressed genes (DEG) during pupa development. Gene ontology (GO) analysis of these DEGs indicated that the genes related to substance metabolism were highly expressed in the early pupa, which participate in the physiological processes of larval tissue disintegration at these stages. In comparison, highly expressed genes in the late pupal stages were mainly associated with substance biosynthesis, consistent with adult organ formation at these stages. There were 27 solute carrier (SLC) genes that were highly expressed during pupa development. We knocked down SLC22A3 at the prepupal stage, demonstrating that silencing SLC22A3 induced a deficiency in pupa stiffness and pigmentation. The RNAi-treated individuals had white and soft pupa, suggesting that this gene has an essential role in pupal development.
-
identification of novel odorant binding protein genes and functional characterization of obp8 in Chilo suppressalis walker
Gene, 2016Co-Authors: Guirong Wang, Fei Li, Ke Yang, Shuanglin DongAbstract:Abstract At the peripheral level of the insect olfaction, odorant binding proteins (OBPs) are thought to bind and transport exogenous hydrophobic volatiles to the odorant receptors (ORs) located on the dendrite membrane of the olfactory neurons. In this study, cDNA sequences of 29 OBP genes from Chilo suppressalis, a notorious rice pest, were identified, with 15 of them being newly reported. The tissue and temporal expression patterns of these CsupOBPs were determined by RT-PCR, revealing that CsupOBP8, 10 and 24 were specifically expressed in the heads at larval stage and in antennae of both sexes at adult stage. In addition, CsupOBP 6, 9 and 18 were expressed in much higher levels in heads than abdomen at the larval stage, while CsupPBP4 and CsupOBP1, 3, 8, 11 and 24 were specifically expressed in antennae of both sexes at the adult stage. Such expression profile strongly suggests olfactory roles of these genes. Furthermore, the function of CsupOBP8 was chosen to be investigated by the competitive fluorescence binding assay. Results showed that CsupOBP8 displayed high binding affinities (Ki = 4.9–15.0 μM) with plant volatile β-ionone, nerolidol, farnesol and 2-hexanone, suggesting that CsupOBP8 plays olfactory roles through binding and transporting the plant volatiles. The study provides an important base for understanding the olfactory mechanisms in C. suppressalis.
-
large number of putative chemoreception and pheromone biosynthesis genes revealed by analyzing transcriptome from ovipositor pheromone glands of Chilo suppressalis
Scientific Reports, 2015Co-Authors: Yanan Zhang, Fei Li, Shuanglin DongAbstract:Large number of putative chemoreception and pheromone biosynthesis genes revealed by analyzing transcriptome from ovipositor-pheromone glands of Chilo suppressalis
-
genome wide analysis reveals the expansion of cytochrome p450 genes associated with xenobiotic metabolism in rice striped stem borer Chilo suppressalis
Biochemical and Biophysical Research Communications, 2014Co-Authors: Baoju Wang, Zan Zhang, Muhammad Faisal Shahzad, Fei LiAbstract:Abstract The Cytochrome P450 (CYP) superfamily is a large group of ancient proteins with enzymatic activities involved in various physiological processes. The rice striped stem borer, Chilo suppressalis, is an important insect pest in rice production. Here, we report the identification and characterization of 77 CYP genes from rice striped stem borer (SSB) through genome and transcriptome sequence analyses. All these CYP genes were confirmed by RT-PCR and direct sequencing. Twenty-eight CYP transcripts have full open reading frame (ORF) and four additional transcripts have a nearly full length coding region. The SSB CYP genes were classified into four clans, the mitochondrial, CYP2, CYP3, and CYP4. Phylogenetic analysis indicated that there was an apparent expansion of the CYP3 clan in insects. The CYP6AB subfamily of the CYP3 clan had nine members in SSB. Evolutionary analysis showed that this subfamily was expanded only in lepidopteran insects. In this study, we identified a new P450 subfamily, CYP321F, which is unique to SSB and located in the genome as tandem repeats. Our work provided a foundation for future studies on the functions and mechanism of P450s in the destructive rice pest.
Shunfan Wu - One of the best experts on this subject based on the ideXlab platform.
-
monitoring and mechanisms of insecticide resistance in Chilo suppressalis lepidoptera crambidae with special reference to diamides
Pest Management Science, 2017Co-Authors: Dandan Zhao, Shuai Zhang, Liqi Zhou, Xin Wang, Shunfan WuAbstract:BACKGROUND The rice stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae), is one of the most economically important pests of rice in Asia. Chemical control remains the most efficient primary means for controlling this pest. RESULTS Significant variations among field populations in their resistance to seven insecticides were observed. The populations exhibited LC50 values that ranged between 0.605 and 108.088 mg AI L−1 for chlorantraniliprole and between 0.046 and 3.919 mg AI L−1 for flubendiamide. The YY14 population collected from Yuyao in Zhejiang Province in 2014 showed a moderate resistance level to the two diamides, i.e. up to 77.6-fold and 42.6-fold for chlorantraniliprole and flubendiamide respectively. Synergism tests and biochemical assays showed no obvious correlations between diamide resistance and three detoxifying enzymes. Sequence comparison of the ryanodine receptor gene between the YY14 resistant population and susceptible population revealed that a glycine to glutamic acid substitution (G4910E) was presented in the YY14 population. CONCLUSION G4910E mutation might be involved in the resistance evolution of C. suppressalis to the diamides. An appropriate insecticide resistance management programme should be established to maintain the effectiveness of the insecticides and to ensure sustainable management. © 2016 Society of Chemical Industry
-
identification and expression profiles of neuropeptides and their g protein coupled receptors in the rice stem borer Chilo suppressalis
Scientific Reports, 2016Co-Authors: Gang Xu, Shunfan Wu, Gongyin Ye, Jia Huang, Guixiang Gu, Ziwen Teng, Qisheng Song, Qi FangAbstract:In insects, neuropeptides play important roles in the regulation of multiple physiological processes by binding to their corresponding receptors, which are primarily G protein-coupled receptors (GPCRs). The genes encoding neuropeptides and their associated GPCRs in the rice stem borer Chilo suppressalis were identified by a transcriptomic analysis and were used to identify potential targets for the disruption of physiological processes and the protection of crops. Forty-three candidate genes were found to encode the neuropeptide precursors for all known insect neuropeptides except for arginine-vasopressin-like peptide (AVLP), CNMamide, neuropeptide-like precursors 2-4 (NPLP2-4) and proctolin. In addition, novel alternative splicing variants of three neuropeptide genes (allatostatin CC, CCHamide 1 and short neuropeptide F) are reported for the first time and 51 putative neuropeptide GPCRs were identified. Phylogenetic analyses demonstrated that 44 of these GPCRs belong to the A-family (or rhodopsin-like), 5 belong to the B-family (or secretin-like) and 2 are leucine-rich repeat-containing GPCRs. These GPCRs and their likely ligands were also described. qRT-PCR analyses revealed the expression profiles of the neuropeptide precursors and GPCR genes in various tissues of C. suppressalis. Our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in Lepidoptera and aid in the design of peptidomimetics, pseudopeptides or small molecules capable of disrupting the physiological processes regulated by these signaling molecules and their receptors.
-
dopamine modulates hemocyte phagocytosis via a d1 like receptor in the rice stem borer Chilo suppressalis
Scientific Reports, 2015Co-Authors: Shunfan Wu, David Stanley, Jia Huang, Gang Xu, Gongyin YeAbstract:Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis
-
susceptibility of the rice stem borer Chilo suppressalis lepidoptera crambidae to flubendiamide in china
Journal of Economic Entomology, 2014Co-Authors: Min Wu, Shunfan Wu, Shuai Zhang, Jianya SuAbstract:ABSTRACT The rice stem borer, Chilo suppressalis (Walker), is an important rice pest in China, and has evolved resistance to several classes of insecticides. Flubendiamide is a phthalic diamide insecticide that shows selective insecticidal activity against lepidopterous insects. The susceptibility of 40 field populations of C. suppressalis, collected in 2011 and 2012 in seven provinces of south-eastern China, to flubendiamide was determined through rice seedling dipping bioassay method. Of these 40 populations, seven populations that were seldom exposed to flubendiamide were used to set up the baseline sensitivity, and the LC50 value was 0.092 mg/L. Variation in susceptibility among the 40 field populations was high (34-fold). The range of mean lethal concentration (LC50) values in response to this chemical was between 0.032 mg/L (FS11) and 1.090 mg/L (JH12) across the populations. Substantial variations of the susceptibility to flubendiamide were detected among different geographic populations. There was...
-
parasitization by cotesia Chilonis influences gene expression in fatbody and hemocytes of Chilo suppressalis
PLOS ONE, 2013Co-Authors: Shunfan Wu, David Stanley, Jia Huang, Qi Fang, Yixiang Qi, Gongyin YeAbstract:Background: During oviposition many parasitoid wasps inject various factors, such as polydnaviruses (PDVs), along with eggs that manipulate the physiology and development of their hosts. These manipulations are thought to benefit the parasites. However, the detailed mechanisms of insect host-parasitoid interactions are not fully understood at the molecular level. Based on recent findings that some parasitoids influence gene expression in their hosts, we posed the hypothesis that parasitization by a braconid wasp, Cotesia Chilonis, influences the expression of genes responsible for development, metabolism and immune functions in the fatbody and hemocytes of its host, Chilo suppressalis. Methodology/Principal Findings: We obtained 39,344,452 reads, which were assembled into 146,770 scaffolds, and 76,016 unigenes. Parasitization impacted gene expression in fatbody and hemocytes. Of these, 8096 fatbody or 5743 hemocyte unigenes were down-regulated, and 2572 fatbody or 1452 hemocyte unigenes were up-regulated. Gene ontology data showed that the majority of the differentially expressed genes are involved in enzyme-regulated activity, binding, transcription regulator activity and catalytic activity. qPCR results show that most anti-microbial peptide transcription levels were up-regulated after parasitization. Expression of bracovirus genes was detected in parasitized larvae with 19 unique sequences identified from six PDV gene families including ankyrin, CrV1 protein, cystatin, early-expressed (EP) proteins, lectin, and protein tyrosine phosphatase. Conclusions: The current study supports our hypothesis that parasitization influences the expression of fatbody and hemocyte genes in the host, C. suppressalis. The general view is that manipulation of host metabolism and immunity benefits the development and emergence of the parasitoid offsprings. The accepted beneficial mechanisms include the direct impact of parasitoid-associated virulence factors such as venom and polydnavirus on host tissues (such as cell damage) and, more deeply, the ability of these factors to influence gene expression. We infer that insect parasitoids generally manipulate their environments, the internal milieu of their hosts.
Yufa Peng - One of the best experts on this subject based on the ideXlab platform.
-
rnai in the striped stem borer Chilo suppressalis establishes a functional role for aminopeptidase n in cry1ab intoxication
Journal of Invertebrate Pathology, 2017Co-Authors: X Y Wang, L X Du, Liang Gong, Yufa PengAbstract:Abstract The striped stem borer, Chilo suppressalis , is a major target pest of transgenic rice expressing the Cry1Ab protein from the bacterium Bacillus thuringiensis (Bt) in China. Evolution of resistance in this pest is a major threat to the durability of Bt rice. Since Bt exerts its activity through binding to specific receptors in the midgut of target insects, identification of functional Cry1Ab receptors in the midgut of C. suppressalis larvae is crucial to evaluate potential resistance mechanisms and develop effective strategies for delaying insect resistance. In this work, we identified the putative Cry1Ab toxin-binding protein, aminopeptidase-N (APN), in the midgut of C. suppressalis by ligand blot and mass spectrometry. After cloning the full-length cDNAs encoding APN isoforms from the C. suppressalis larval midgut, we studied their spatiotemporal expression in different gut tissues and developmental stages. Furthermore, RNA interference (RNAi) against C. suppressalis aminopeptidases (CsAPNs) was employed to illustrate a functional role for CsAPNs in Cry1Ab toxicity to C. suppressalis larvae using injection and oral delivery of Stealth™ siRNA. Down-regulating the expression of CsAPNs by RNAi was closely associated with reduced susceptibility of C. suppressalis to Cry1Ab. These data provide the first direct evidence that CsAPNs participate in the mode of Cry1Ab action and may act as the functional receptor of Cry1A in C. suppressalis larvae.
-
comparison of three transgenic bt rice lines for insecticidal protein expression and resistance against a target pest Chilo suppressalis lepidoptera crambidae
Insect Science, 2016Co-Authors: Yanan Wang, Lanzhi Han, Yanmin Liu, Hongxia Hua, Yufa PengAbstract:Two transgenic rice lines (T2A-1 and T1C-19b) expressing cry2A and cry1C genes, respectively, were developed in China, targeting lepidopteran pests including Chilo suppressalis (Walker) (Lepidoptera: Crambidae). The seasonal expression of Cry proteins in different tissues of the rice lines and their resistance to C. suppressalis were assessed in comparison to a Bt rice line expressing a cry1Ab/Ac fusion gene, Huahui 1, which has been granted a biosafety certificate. In general, levels of Cry proteins were T2A-1 > Huahui 1 > T1C-19b among rice lines, and leaf > stem > root among rice tissues. The expression patterns of Cry protein in the rice line plants were similar: higher level at early stages than at later stages with an exception that high Cry1C level in T1C-19b stems at the maturing stage. The bioassay results revealed that the three transgenic rice lines exhibited significantly high resistance against C. suppressalis larvae throughout the rice growing season. According to Cry protein levels in rice tissues, the raw and corrected mortalities of C. suppressalis caused by each Bt rice line were the highest in the seedling and declined through the jointing stage with an exception for T1C-19b providing an excellent performance at the maturing stage. By comparison, T1C-19b exhibited more stable and greater resistance to C. suppressalis larvae than T2A-1, being close to Huahui 1. The results suggest cry1C is an ideal Bt gene for plant transformation for lepidopteran pest control, and T1C-19b is a promising Bt rice line for commercial use for tolerating lepidopteran rice pests.
-
binding site concentration explains the differential susceptibility of Chilo suppressalis and sesamia inferens to cry1a producing rice
Applied and Environmental Microbiology, 2014Co-Authors: Fajun Chen, Juan Luis Juratfuentes, Yufa PengAbstract:ABSTRACT Chilo suppressalis and Sesamia inferens are two important lepidopteran rice pests that occur concurrently during outbreaks in paddy fields in the main rice-growing areas of China. Previous and current field tests demonstrate that the transgenic rice line Huahui 1 (HH1) producing a Cry1Ab-Cry1Ac hybrid toxin from the bacterium Bacillus thuringiensis reduces egg and larval densities of C. suppressalis but not of S. inferens. This differential susceptibility to HH1 rice correlates with the reduced susceptibility to Cry1Ab and Cry1Ac toxins in S. inferens larvae compared to C. suppressalis larvae. The goal of this study was to identify the mechanism responsible for this differential susceptibility. In saturation binding assays, both Cry1Ab and Cry1Ac toxins bound with high affinity and in a saturable manner to midgut brush border membrane vesicles (BBMV) from C. suppressalis and S. inferens larvae. While binding affinities were similar, a dramatically lower concentration of Cry1A toxin binding sites was detected for S. inferens BBMV than for C. suppressalis BBMV. In contrast, no significant differences between species were detected for Cry1Ca toxin binding to BBMV. Ligand blotting detected BBMV proteins binding Cry1Ac or Cry1Ca toxins, some of them unique to C. suppressalis or S. inferens. These data support that reduced Cry1A binding site concentration is associated with a lower susceptibility to Cry1A toxins and HH1 rice in S. inferens larvae than in C. suppressalis larvae. Moreover, our data support Cry1Ca as a candidate for pyramiding efforts with Cry1A-producing rice to extend the activity range and durability of this technology against rice stem borers.
-
expression of cry1ab protein in a marker free transgenic bt rice line and its efficacy in controlling a target pest Chilo suppressalis lepidoptera crambidae
Environmental Entomology, 2014Co-Authors: Yanan Wang, Yunhe Li, Feng Wang, Lei Zhang, Yufa PengAbstract:ABSTRACT A marker-free Bt transgenic rice line, mfb-MH86, was recently developed in China, which contains a cry1Ab gene driven by a ubiquitin promoter. This Bt gene confers resistance to a range of lepidopteran species, including the striped stem borer, Chilo suppressalis (Walker). The expression of Cry1Ab protein in mfb-MH86 leaves, stems and leaf sheaths (hereinafter referred to as stems), and roots was evaluated throughout the rice-growing season using an enzyme-linked immunosorbent assay. In addition, mfb-MH86 resistance to C. suppressalis, a major pest of rice, was evaluated in a laboratory bioassay with field-collected rice stems. Cry1Ab protein levels of mfb-MH86 were highest in leaves (9.71–34.09 µg/g dry weight [DW]), intermediate in stems (7.66–18.51 µg/g DW), and lowest in roots (1.95–13.40 µg/g DW). In all tissues, Cry1Ab levels in mfb-MH86 were higher in seedling and tillering stages than in subsequent growth stages. In the laboratory bioassay, mortality of C. suppressalis after 6 d of feedin...
-
new artificial diet for continuous rearing of Chilo suppressalis lepidoptera crambidae
Annals of The Entomological Society of America, 2012Co-Authors: Shengbing Li, Yufa PengAbstract:The Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), is an important rice, Oryza sativa L., pest in China and difficult to control with conventional pest management. To develop and optimize integrated pest management strategies, efficient and economic artificial diet and rearing protocols are desirable. A new artificial diet based on soybean, Glycine max (L.) Merr., powder and fresh water bamboo, Zizania caduciflora (Turcz.) Hand.-Mazz, was formulated and rearing technique was developed. Fitness parameters including larval development, immature survival, pupal weight, pupation, adult emergence, egg hatchability, and oviposition were measured to evaluate the performance of C. suppressalis fed on the diet over 15 successive generations. C. suppressalis reared on the artificial diet showed better performance with shorter developmental stage, similar larval survival rate and fecundity, and heavier pupae compared with that fed on rice plants and fresh water bamboo. A positive correlation was observed between number of eggs laid per female and number of generations reared on the diet. Larval development time tended to be shortened with successive rearing on the artificial diet. These results indicated that C. suppressalis adapted well to the artificial diet and successive rearing conditions. The diet could serve as a viable alternative to natural host plants for consecutive rearing of the insect. In addition, the diet is inexpensive (US$1.5/1,000 g) and easy to make. The better preserve ability of the diet required only one diet replacement during the rearing process. The successful development of the diet and rearing technique provides a very useful tool for refining stem borer pest management techniques.
Jiaan Cheng - One of the best experts on this subject based on the ideXlab platform.
-
construction and analysis of antennal cdna library from rice striped stem borer Chilo suppressalis walker lepidoptera pyralidae and expression profiles of putative odorant binding protein and chemosensory protein genes
Archives of Insect Biochemistry and Physiology, 2015Co-Authors: Zhongjun Gong, Wenwu Zhou, Su Liu, Yandong Jiang, Qingmei Liang, Jiaan ChengAbstract:In this study, we constructed a high-quality cDNA library from the antennae of the Chilo suppressalis (Walker) (Lepidoptera: Pyralidae). A total of 1,235 colonies with inserts greater than 0.7 kb were sequenced and analyzed. Homology searching coupled with bioinformatics analysis identified 15 and 7 cDNA sequences, respectively, encoding putative odorant-binding proteins (OBPs) and chemosensory proteins (CSPs). A phylogenetic tree of CsupCSPs showed that each CsupCSP has orthologs in Manduca sexta and Bombyx mori with strong bootstrapping support. One CSP was either very specific or more related to the CSPs of another species than to conspecific CSP. The expression profiles of the OBPs and CSPs in different tissues were measured by real-time quantitative PCR. The results revealed that of the 11 OBP genes, the transcript levels of CsupOBP1, CsupOBP5, and CsupOBP7 were higher in both male and female antennae than those in other tissues. And CsupCSP7 was highly expressed in both male and female antennae. Based on these results, the possible physiological functions of CsupOBPs and CsupCSPs were discussed.
-
genome wide transcriptional changes and defence related chemical profiling of rice in response to infestation by the rice striped stem borer Chilo suppressalis
Physiologia Plantarum, 2011Co-Authors: Guoxin Zhou, Xia Wang, Ran Li, Jiaan ChengAbstract:: How rice defends itself against pathogen infection is well documented, but little is known about how it defends itself against herbivore attack. We measured changes in the transcriptome and chemical profile of rice when the plant is infested by the striped stem borer (SSB) Chilo suppressalis. Infestation by SSBs resulted in changes in the expression levels of 4545 rice genes; this number accounts for about 8% of the genome and is made up of 18 functional groups with broad functions. The largest group comprised genes involved in metabolism, followed by cellular transport, transcription and cellular signaling. Infestation by SSBs modulated many genes responsible for the biosynthesis of plant hormones and plant signaling. Jasmonic acid (JA), salicylic acid (SA) and ethylene were the major hormones that shaped the SSB-induced defence responses of rice. Many secondary signal transduction components, such as those involved in Ca²⁺ signaling and G-protein signaling, receptor and non-receptor protein kinases, and transcription factors were involved in the SSB-induced responses of rice. Photosynthesis and ATP synthesis from photophosphorylation were restricted by SSB feeding. In addition, SSB infestation induced the accumulation of defence compounds, including trypsin proteinase inhibitors (TrypPIs) and volatile organic compounds. These results demonstrate that SSB-induced defences required rice to reconfigure a wide variety of its metabolic, physiological and biochemical processes.
-
cloning expression and functional analysis of a general odorant binding protein 2 gene of the rice striped stem borer Chilo suppressalis walker lepidoptera pyralidae
Insect Molecular Biology, 2009Co-Authors: Zhongjun Gong, Wenwu Zhou, Hang Yu, Chuanxi Zhang, Jiaan ChengAbstract:A full-length cDNA encoding a general odorant binding protein 2 (GOBP2) was cloned from the antennae of the rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae), by the combination of reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends PCR (RACE-PCR). The cDNA contains a 489 bp open reading frame, which encodes a 162 amino acid protein, termed as Ch. suppressalis GOBP2 (CsupGOBP2). CsupGOBP2 is similar in the number of amino acids and protein sequence to GOBP2s in other species of Lepidoptera. RT-PCR results showed that CsupGOBP2 mRNA was highly expressed in the adult antennae of both females and males, as was CsupGOBP2 protein as revealed by Western blot analysis. CsupGOBP2 expressed in Escherichia coli was purified by affinity chromatography, refolding and gel filtration from the inclusion body. Fluorescence emission spectra and competitive binding assays by using N-phenyl-1-naphthylamine as first binding ligand and odorants as potential competitors revealed that the CsupGOBP2 protein has significant affinity to cis -11-hexadecenal (Z11‐16:Ald), the main component of Ch. suppressalis pheromone and to laurinaldehyd and benzaldehyde, two general plant volatile aldehydes.
Gongyin Ye - One of the best experts on this subject based on the ideXlab platform.
-
identification and expression profiles of neuropeptides and their g protein coupled receptors in the rice stem borer Chilo suppressalis
Scientific Reports, 2016Co-Authors: Gang Xu, Shunfan Wu, Gongyin Ye, Jia Huang, Guixiang Gu, Ziwen Teng, Qisheng Song, Qi FangAbstract:In insects, neuropeptides play important roles in the regulation of multiple physiological processes by binding to their corresponding receptors, which are primarily G protein-coupled receptors (GPCRs). The genes encoding neuropeptides and their associated GPCRs in the rice stem borer Chilo suppressalis were identified by a transcriptomic analysis and were used to identify potential targets for the disruption of physiological processes and the protection of crops. Forty-three candidate genes were found to encode the neuropeptide precursors for all known insect neuropeptides except for arginine-vasopressin-like peptide (AVLP), CNMamide, neuropeptide-like precursors 2-4 (NPLP2-4) and proctolin. In addition, novel alternative splicing variants of three neuropeptide genes (allatostatin CC, CCHamide 1 and short neuropeptide F) are reported for the first time and 51 putative neuropeptide GPCRs were identified. Phylogenetic analyses demonstrated that 44 of these GPCRs belong to the A-family (or rhodopsin-like), 5 belong to the B-family (or secretin-like) and 2 are leucine-rich repeat-containing GPCRs. These GPCRs and their likely ligands were also described. qRT-PCR analyses revealed the expression profiles of the neuropeptide precursors and GPCR genes in various tissues of C. suppressalis. Our study provides fundamental information that may further our understanding of neuropeptidergic signaling systems in Lepidoptera and aid in the design of peptidomimetics, pseudopeptides or small molecules capable of disrupting the physiological processes regulated by these signaling molecules and their receptors.
-
dopamine modulates hemocyte phagocytosis via a d1 like receptor in the rice stem borer Chilo suppressalis
Scientific Reports, 2015Co-Authors: Shunfan Wu, David Stanley, Jia Huang, Gang Xu, Gongyin YeAbstract:Dopamine modulates hemocyte phagocytosis via a D1-like receptor in the rice stem borer, Chilo suppressalis
-
parasitization by cotesia Chilonis influences gene expression in fatbody and hemocytes of Chilo suppressalis
PLOS ONE, 2013Co-Authors: Shunfan Wu, David Stanley, Jia Huang, Qi Fang, Yixiang Qi, Gongyin YeAbstract:Background: During oviposition many parasitoid wasps inject various factors, such as polydnaviruses (PDVs), along with eggs that manipulate the physiology and development of their hosts. These manipulations are thought to benefit the parasites. However, the detailed mechanisms of insect host-parasitoid interactions are not fully understood at the molecular level. Based on recent findings that some parasitoids influence gene expression in their hosts, we posed the hypothesis that parasitization by a braconid wasp, Cotesia Chilonis, influences the expression of genes responsible for development, metabolism and immune functions in the fatbody and hemocytes of its host, Chilo suppressalis. Methodology/Principal Findings: We obtained 39,344,452 reads, which were assembled into 146,770 scaffolds, and 76,016 unigenes. Parasitization impacted gene expression in fatbody and hemocytes. Of these, 8096 fatbody or 5743 hemocyte unigenes were down-regulated, and 2572 fatbody or 1452 hemocyte unigenes were up-regulated. Gene ontology data showed that the majority of the differentially expressed genes are involved in enzyme-regulated activity, binding, transcription regulator activity and catalytic activity. qPCR results show that most anti-microbial peptide transcription levels were up-regulated after parasitization. Expression of bracovirus genes was detected in parasitized larvae with 19 unique sequences identified from six PDV gene families including ankyrin, CrV1 protein, cystatin, early-expressed (EP) proteins, lectin, and protein tyrosine phosphatase. Conclusions: The current study supports our hypothesis that parasitization influences the expression of fatbody and hemocyte genes in the host, C. suppressalis. The general view is that manipulation of host metabolism and immunity benefits the development and emergence of the parasitoid offsprings. The accepted beneficial mechanisms include the direct impact of parasitoid-associated virulence factors such as venom and polydnavirus on host tissues (such as cell damage) and, more deeply, the ability of these factors to influence gene expression. We infer that insect parasitoids generally manipulate their environments, the internal milieu of their hosts.
-
molecular cloning and pharmacological characterisation of a tyramine receptor from the rice stem borer Chilo suppressalis walker
Pest Management Science, 2013Co-Authors: Shunfan Wu, Jia Huang, Gongyin YeAbstract:BACKGROUND: Tyramine (TA) and octopamine (OA) are considered to be the invertebrate counterparts of the vertebrate adrenergic transmitters. Because these two phenolamines are the only biogenic amines whose physiological significance is presumably restricted to invertebrates, the attention of pharmacologists has been focused on the corresponding receptors, which are believed to represent promising targets for novel insecticides. For example, the formamidine pesticides, such as chlordimeform and amitraz, have been shown to activate OA receptors. RESULTS: A full-length cDNA (designated CsTyR1) from the rice stem borer, Chilo suppressalis (Walker), has been obtained through homology cloning in combination with rapid amplification of cDNA ends/polymerase chain reaction (RACE-PCR). The mRNA of CsTyR1 is present in various tissues, including hemocytes, fat body, midgut, Malpighian tubules, nerve cord and epidermis, and it is found predominantly in the larval nerve cord with 16-80-fold enrichment compared with other tissues. The authors generated a HEK 293 cell line stably expressing CsTyR1 in order to examine functional and pharmacological properties of this receptor. Both TA and OA at 0.01-100 µM can reduce forskolin-stimulated intracellular cAMP levels in a dose-dependent manner (TA, EC(50) = 369 nM; OA, EC(50) = 978 nM). In agonist assays, activation of CsTyR1 by clonidine and amitraz but not by naphazoline and chlordimeform can also significantly inhibit forskolin-stimulated cAMP production. The inhibitory effect of TA at 10 µM is eliminated by coincubation with yohimbine, phentolamine or chlorpromazine (each 10 µM). CONCLUSION: This study represents a comprehensive molecular and pharmacological characterisation of a tyramine receptor in the rice stem borer.
-
characterization of a β adrenergic like octopamine receptor from the rice stem borer Chilo suppressalis
The Journal of Experimental Biology, 2012Co-Authors: Shunfan Wu, Jia Huang, Gongyin YeAbstract:SUMMARY Octopamine, the invertebrate counterpart of adrenaline and noradrenaline, plays a key role in regulation of many physiological and behavioral processes in insects. It modulates these functions through binding to specific octopamine receptors, which are typical rhodopsin-like G-protein coupled receptors. A cDNA encoding a seven-transmembrane receptor was cloned from the nerve cord of the rice stem borer, Chilo suppressalis , viz. CsOA2B2 , which shares high sequence similarity to CG6989, a Drosophila β-adrenergic-like octopamine receptor (DmOctβ2R). We generated an HEK-293 cell line that stably expresses CsOA2B2 in order to examine the functional and pharmacological properties of this receptor. Activation of CsOA2B2 by octopamine increased the production of cAMP in a dose-dependent manner (EC 50 =2.33 nmol l –1 ), with a maximum response at 100 nmol l –1 . Tyramine also activated the receptor but with much less potency than octopamine. Dopamine and serotonin had marginal effects on cAMP production. Using a series of known agonists and antagonists for octopamine receptors, we observed a rather unique pharmacological profile for CsOA2B2 through measurements of cAMP. The rank order of potency of the agonists was naphazoline > clonidine. The activated effect of octopamine is abolished by co-incubation with phentolamine, mianserin or chlorpromazine. Using in vivo pharmacology, CsOA2B2 antagonists mianserin and phentolamine impaired the motor ability of individual rice stem borers. The results of the present study are important for a better functional understanding of this receptor as well as for practical applications in the development of environmentally sustainable pesticides.