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Domenico Ribatti - One of the best experts on this subject based on the ideXlab platform.

  • the chick embryo Chorioallantoic Membrane as an in vivo experimental model to study multiple myeloma
    The Enzymes, 2019
    Co-Authors: Domenico Ribatti, Roberto Tamma
    Abstract:

    Abstract The chick embryo Chorioallantoic Membrane (CAM) has long been used as an in vivo assay for the study of tumor angiogenesis because when tumor grafts are placed at day 6–10 days of incubation the chick's immunocompetent system is not fully developed and the conditions for rejection have not been yet established. All studies for mammalian neoplasms, including multiple myeloma, have utilized tumor cell lines, tumor bioptic specimens, cell suspensions derived from tumors, mouse tumor xenografts bioptic specimens. CAM can also be used to study the effects of anti-angiogenic molecules on tumor cell suspensions of tumor bioptic specimens. This review article summarizes and discussed our experience concerning the use of the CAM to study multiple myeloma.

  • the chick embryo Chorioallantoic Membrane as an in vivo experimental model to study human neuroblastoma
    Journal of Cellular Physiology, 2019
    Co-Authors: Domenico Ribatti, Roberto Tamma
    Abstract:

    : The chick embryo Chorioallantoic Membrane (CAM) has long been a favored system for the study of tumor angiogenesis because at the stage of development when generally tumor grafts are placed (6-10 days of incubation), the chick's immunocompetent system is not fully developed and the conditions for rejection have not yet been established. All studies for mammalian neoplasms, including neuroblastoma, have used tumor cell lines, tumor bioptic specimens, cell suspensions derived from tumors, and mouse tumor xenografts bioptic specimens. CAM can also be used to study the effects of antiangiogenic molecules on tumor cell suspensions of tumor bioptic specimens. This review article summarizes and discusses the literature data on the use of the CAM as an in vivo experimental model to study human neuroblastoma.

  • the chick embryo Chorioallantoic Membrane
    In Vivo Models to Study Angiogenesis, 2018
    Co-Authors: Domenico Ribatti
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of proangiogenic and antiangiogenic natural and synthetic molecules. The CAM is a favored system for the study of tumor angiogenesis and metastasis, because at this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established. Tumors remain avascular for 72 h, after which they are penetrated by new blood vessels and begin a phase of rapid growth. The rate of growth during this vascular phase is greater for implants on days 5–6 and decreases for later days of implantation. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.

  • the chick embryo Chorioallantoic Membrane cam assay
    Reproductive Toxicology, 2017
    Co-Authors: Domenico Ribatti
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of pro-angiogenic and anti-angiogenic natural molecules. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because a this stage the chick immunocompetence system is not fully developed and the conditions for rejection have not been established.

  • The chick embryo Chorioallantoic Membrane (CAM) assay
    Reproductive Toxicology, 2017
    Co-Authors: Domenico Ribatti
    Abstract:

    During avian development the mesodermal layers of the allantois and chorion fuse to form the Chorioallantoic Membrane (CAM). This structure rapidly expands generating a rich vascular network that provides an interface for gas and waste exchange. The CAM allows to study tissue grafts, tumor growth and metastasis, drugs delivery and toxicologic analysis, and angiogenic and anti-angiogenic molecules. The CAM is relatively simple, quick, and low-cost model that allows screening of a large number of pharmacological samples in a short time; does not require administrative procedures for obtaining ethics committee approval for animal experimentation. Moreover, being naturally immunodeficient, the chick embryo may receive transplantations from different tissues and species, without immune responses.

F Dammacco - One of the best experts on this subject based on the ideXlab platform.

  • the chick embryo Chorioallantoic Membrane as a model for in vivo research on angiogenesis
    The International Journal of Developmental Biology, 2004
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane that is commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Quantitative or semiquantitative methods may be used to evaluate the amount of angiogenesis and anti-angiogenesis. Thanks to the CAM system, angiogenesis could be investigated in association with normal, inflammatory and tumor tissues, and soluble factors inducing angiogenic or anti-angiogenic effects could be identified. Rabbit cornea provides an alternative in vivo system, but CAM appears to be easier to handle and less expensive. Moreover, CAM can be used with very few limitations.

  • mast cells and their secretory granules are angiogenic in the chick embryo Chorioallantoic Membrane
    Clinical & Experimental Allergy, 2001
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Luisa Roncali, Enrico Crivellato, Luigi Candussio, F Dammacco
    Abstract:

    Background Many data suggest that the density of mast cells is highly correlated with the extent of both normal and pathological angiogenesis. Objective In this study we have compared in an in vivo assay, the chick embryo Chorioallantoic Membrane, the angiogenic potential of mast cell suspensions isolated from rats, degranulated mast cells and their secretory granules. Methods Gelatin sponges adsorbed with cell suspensions of rat mast cells, degranulated mast cells and their secretory granules were implanted on the top of the Chorioallantoic Membrane at day 8 of incubation. At day 12 the angiogenic response was evaluated macroscopically, microscopically and by a morphometric method of ‘point counting’. Results Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the Chorioallantoic Membrane. The addition of antifibroblast growth factor-2 or antivascular endothelial growth factor antibodies reduced the angiogenic response of both mast cells and their secretory granules by 50% and 30%, respectively. Conclusion These data support the evidence that the angiogenic properties of mast cells depend on the angiogenic molecules contained in their secretory granules and indicate that fibroblast growth factor-2 and vascular endothelial growth factor are the angiogenic cytokines primarily and perhaps synergistically responsible for this vasoproliferative activity.

  • the chick embryo Chorioallantoic Membrane as a model for in vivo research on anti angiogenesis
    Current Pharmaceutical Biotechnology, 2000
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    Anti-angiogenesis, i.e.inhibition of blood vessel growth, is being investigated as a way to prevent the growth of tumors and other angiogenesis-dependent diseases. Pharmacological inhibition interferes with the angiogenic cascade or the immature neovasculature with synthetic or semi-synthetic substances, endogenous inhibitors or biological antagonists. The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Angiogenesis or anti-angiogenesis is evaluated quantitatively or semiquantitatively. The fields of application of CAM in the study of anti-angiogenesis, including our personal experience, are illustrated in this paper.

Angelo Vacca - One of the best experts on this subject based on the ideXlab platform.

  • the gelatin sponge Chorioallantoic Membrane assay
    Nature Protocols, 2006
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Marco Presta
    Abstract:

    Here we present a method for the quantification of angiogenesis and antiangiogenesis in the chick embryo Chorioallantoic Membrane (CAM) based on the implantation of a gelatin sponge on the top of the growing CAM on day 8 of development. After implantation, the sponge is treated with a stimulator of blood vessel formation in the absence or presence of an angiogenesis inhibitor. On day 12, blood vessels that are growing into the sponge are counted at macroscopic and microscopic levels. The estimated timeline for carrying out this protocol is 10 d. The presence of a vascular network in the CAM requires a careful analysis to distinguish new capillaries from pre-existing ones. This limitation does not occur in the avascular cornea assay, which may also take advantage of different genetic backgrounds when carried out in transgenic or knockout mice. Nevertheless, the gelatin sponge–CAM assay is simple, inexpensive and suitable for large-scale screening. *Note: In the version of the article initially published online, references 6 and 7 were incorrect. The correct references are: 6. Serbedzija, G.N., Flynn, E. & Willet, C.E. Zebrafish angiogenesis: a new model for drug screening. Angiogenesis 3, 519–528 (2000). 7. Ribatti, D., Vacca, A., Roncali, L. & Dammacco, F. The chick embryo Chorioallantoic Membrane as a model for in vivo research on angiogenesis. Int. J. Dev. Biol. 40, 1189–1897 (1996). The error has been corrected in all versions of the article.

  • the chick embryo Chorioallantoic Membrane as a model for in vivo research on angiogenesis
    The International Journal of Developmental Biology, 2004
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane that is commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Quantitative or semiquantitative methods may be used to evaluate the amount of angiogenesis and anti-angiogenesis. Thanks to the CAM system, angiogenesis could be investigated in association with normal, inflammatory and tumor tissues, and soluble factors inducing angiogenic or anti-angiogenic effects could be identified. Rabbit cornea provides an alternative in vivo system, but CAM appears to be easier to handle and less expensive. Moreover, CAM can be used with very few limitations.

  • Chorioallantoic Membrane capillary bed: a useful target for studying angiogenesis and anti-angiogenesis in vivo.
    The Anatomical record, 2001
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Luisa Roncali, Peter H. Burri, Valentin Djonov
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane that is commonly used in vivo to study both angiogenesis and anti-angiogenesis. This review 1) summarizes the current knowledge about the structure of the CAM's capillary bed; 2) discusses the controversy about the existence of a single blood sinus or a capillary plexus underlying the chorionic epithelium; 3) describes a new model of the CAM vascular growth, namely the intussusceptive mode; 4) reports findings regarding the role played by endogenous fibroblast growth factor-2 in CAM vascularization; and 5) addresses the use and limitations of the CAM as a model for studying angiogenesis and anti-angiogenesis.

  • mast cells and their secretory granules are angiogenic in the chick embryo Chorioallantoic Membrane
    Clinical & Experimental Allergy, 2001
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Luisa Roncali, Enrico Crivellato, Luigi Candussio, F Dammacco
    Abstract:

    Background Many data suggest that the density of mast cells is highly correlated with the extent of both normal and pathological angiogenesis. Objective In this study we have compared in an in vivo assay, the chick embryo Chorioallantoic Membrane, the angiogenic potential of mast cell suspensions isolated from rats, degranulated mast cells and their secretory granules. Methods Gelatin sponges adsorbed with cell suspensions of rat mast cells, degranulated mast cells and their secretory granules were implanted on the top of the Chorioallantoic Membrane at day 8 of incubation. At day 12 the angiogenic response was evaluated macroscopically, microscopically and by a morphometric method of ‘point counting’. Results Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the Chorioallantoic Membrane. The addition of antifibroblast growth factor-2 or antivascular endothelial growth factor antibodies reduced the angiogenic response of both mast cells and their secretory granules by 50% and 30%, respectively. Conclusion These data support the evidence that the angiogenic properties of mast cells depend on the angiogenic molecules contained in their secretory granules and indicate that fibroblast growth factor-2 and vascular endothelial growth factor are the angiogenic cytokines primarily and perhaps synergistically responsible for this vasoproliferative activity.

  • the chick embryo Chorioallantoic Membrane as a model for in vivo research on anti angiogenesis
    Current Pharmaceutical Biotechnology, 2000
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    Anti-angiogenesis, i.e.inhibition of blood vessel growth, is being investigated as a way to prevent the growth of tumors and other angiogenesis-dependent diseases. Pharmacological inhibition interferes with the angiogenic cascade or the immature neovasculature with synthetic or semi-synthetic substances, endogenous inhibitors or biological antagonists. The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Angiogenesis or anti-angiogenesis is evaluated quantitatively or semiquantitatively. The fields of application of CAM in the study of anti-angiogenesis, including our personal experience, are illustrated in this paper.

Luisa Roncali - One of the best experts on this subject based on the ideXlab platform.

  • the chick embryo Chorioallantoic Membrane as a model for in vivo research on angiogenesis
    The International Journal of Developmental Biology, 2004
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane that is commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Quantitative or semiquantitative methods may be used to evaluate the amount of angiogenesis and anti-angiogenesis. Thanks to the CAM system, angiogenesis could be investigated in association with normal, inflammatory and tumor tissues, and soluble factors inducing angiogenic or anti-angiogenic effects could be identified. Rabbit cornea provides an alternative in vivo system, but CAM appears to be easier to handle and less expensive. Moreover, CAM can be used with very few limitations.

  • aquaporin 1 expression in the chick embryo Chorioallantoic Membrane
    Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology, 2002
    Co-Authors: Domenico Ribatti, Beatrice Nico, Luisa Roncali, Antonio Frigeri, Grazia Paola Nicchia, Michela De Giorgis, Maria Svelto
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is commonly used in vivo to study both angiogenesis and anti-angiogenesis. Rapid Membrane water transport is mediated by a family of molecular water channels, called aquaporins (AQPs), which have been identified in the epithelial and endothelial cells of higher vertebrates. AQP1, expressed in adsorptive and secretory epithelia, is also expressed in endothelial cells of capillaries and arteries. Its mRNA has been found in vascular smooth muscle cells (VSMCs) of arteries and capillaries, as well as in a subset of VSMCs of human atherosclerotic plaques. This study investigated the developmental expression of AQP1 in the chick CAM by Western blot and immunohistochemistry. Western blot results show that a major nonglycosylated band was observed with electrophoretic mobility of approximately 28 kDa in the three developmental stages examined. Immunohistochemistry data demonstrate that AQP1 was clearly expressed in the ectodermal and endodermal epithelia, the vascular endothelium, and the VSMCs. Because little information is available on the behavior of microvessel AQP1 during angiogenesis in normal and pathological conditions, our data relative to the pattern of expression of AQP1 in CAM blood vessels in normal conditions may be considered a useful tool to further investigate its modifications in several experimental conditions implying a stimulation or an inhibition of angiogenesis in the CAM assay. Anat Rec 268:85–89, 2002. © 2002 Wiley-Liss, Inc.

  • Chorioallantoic Membrane capillary bed: a useful target for studying angiogenesis and anti-angiogenesis in vivo.
    The Anatomical record, 2001
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Luisa Roncali, Peter H. Burri, Valentin Djonov
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane that is commonly used in vivo to study both angiogenesis and anti-angiogenesis. This review 1) summarizes the current knowledge about the structure of the CAM's capillary bed; 2) discusses the controversy about the existence of a single blood sinus or a capillary plexus underlying the chorionic epithelium; 3) describes a new model of the CAM vascular growth, namely the intussusceptive mode; 4) reports findings regarding the role played by endogenous fibroblast growth factor-2 in CAM vascularization; and 5) addresses the use and limitations of the CAM as a model for studying angiogenesis and anti-angiogenesis.

  • mast cells and their secretory granules are angiogenic in the chick embryo Chorioallantoic Membrane
    Clinical & Experimental Allergy, 2001
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Luisa Roncali, Enrico Crivellato, Luigi Candussio, F Dammacco
    Abstract:

    Background Many data suggest that the density of mast cells is highly correlated with the extent of both normal and pathological angiogenesis. Objective In this study we have compared in an in vivo assay, the chick embryo Chorioallantoic Membrane, the angiogenic potential of mast cell suspensions isolated from rats, degranulated mast cells and their secretory granules. Methods Gelatin sponges adsorbed with cell suspensions of rat mast cells, degranulated mast cells and their secretory granules were implanted on the top of the Chorioallantoic Membrane at day 8 of incubation. At day 12 the angiogenic response was evaluated macroscopically, microscopically and by a morphometric method of ‘point counting’. Results Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the Chorioallantoic Membrane. The addition of antifibroblast growth factor-2 or antivascular endothelial growth factor antibodies reduced the angiogenic response of both mast cells and their secretory granules by 50% and 30%, respectively. Conclusion These data support the evidence that the angiogenic properties of mast cells depend on the angiogenic molecules contained in their secretory granules and indicate that fibroblast growth factor-2 and vascular endothelial growth factor are the angiogenic cytokines primarily and perhaps synergistically responsible for this vasoproliferative activity.

  • the chick embryo Chorioallantoic Membrane as a model for in vivo research on anti angiogenesis
    Current Pharmaceutical Biotechnology, 2000
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    Anti-angiogenesis, i.e.inhibition of blood vessel growth, is being investigated as a way to prevent the growth of tumors and other angiogenesis-dependent diseases. Pharmacological inhibition interferes with the angiogenic cascade or the immature neovasculature with synthetic or semi-synthetic substances, endogenous inhibitors or biological antagonists. The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Angiogenesis or anti-angiogenesis is evaluated quantitatively or semiquantitatively. The fields of application of CAM in the study of anti-angiogenesis, including our personal experience, are illustrated in this paper.

Beatrice Nico - One of the best experts on this subject based on the ideXlab platform.

  • tryptase and chymase are angiogenic in vivo in the Chorioallantoic Membrane assay
    The International Journal of Developmental Biology, 2011
    Co-Authors: Domenico Ribatti, Beatrice Nico, Girolamo Ranieri, Vincenzo Benagiano, Enrico Crivellato
    Abstract:

    Human mast cells (MCs) are divided in two types depending on the expression of tryptase and chymase in their granules. Literature data indicate that both tryptase and chymase are angiogenic, but there is currently no evidence of their direct angiogenic activity in vivo. In this study, we have investigated the capacity of tryptase and chymase to promote vasoproliferation in chick embryo Chorioallantoic Membrane (CAM), a well established in vivo assay to study angiogenesis and anti-angiogenesis. The results showed that both tryptase and chymase stimulate angiogenesis and that the response is similar to that obtained with vascular endothelial growth factor (VEGF), a well-known angiogenic cytokine, and confirm the angiogenic activity of these two proteases stored in MC granules.

  • the gelatin sponge Chorioallantoic Membrane assay
    Nature Protocols, 2006
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Marco Presta
    Abstract:

    Here we present a method for the quantification of angiogenesis and antiangiogenesis in the chick embryo Chorioallantoic Membrane (CAM) based on the implantation of a gelatin sponge on the top of the growing CAM on day 8 of development. After implantation, the sponge is treated with a stimulator of blood vessel formation in the absence or presence of an angiogenesis inhibitor. On day 12, blood vessels that are growing into the sponge are counted at macroscopic and microscopic levels. The estimated timeline for carrying out this protocol is 10 d. The presence of a vascular network in the CAM requires a careful analysis to distinguish new capillaries from pre-existing ones. This limitation does not occur in the avascular cornea assay, which may also take advantage of different genetic backgrounds when carried out in transgenic or knockout mice. Nevertheless, the gelatin sponge–CAM assay is simple, inexpensive and suitable for large-scale screening. *Note: In the version of the article initially published online, references 6 and 7 were incorrect. The correct references are: 6. Serbedzija, G.N., Flynn, E. & Willet, C.E. Zebrafish angiogenesis: a new model for drug screening. Angiogenesis 3, 519–528 (2000). 7. Ribatti, D., Vacca, A., Roncali, L. & Dammacco, F. The chick embryo Chorioallantoic Membrane as a model for in vivo research on angiogenesis. Int. J. Dev. Biol. 40, 1189–1897 (1996). The error has been corrected in all versions of the article.

  • aquaporin 1 expression in the chick embryo Chorioallantoic Membrane
    Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology, 2002
    Co-Authors: Domenico Ribatti, Beatrice Nico, Luisa Roncali, Antonio Frigeri, Grazia Paola Nicchia, Michela De Giorgis, Maria Svelto
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is commonly used in vivo to study both angiogenesis and anti-angiogenesis. Rapid Membrane water transport is mediated by a family of molecular water channels, called aquaporins (AQPs), which have been identified in the epithelial and endothelial cells of higher vertebrates. AQP1, expressed in adsorptive and secretory epithelia, is also expressed in endothelial cells of capillaries and arteries. Its mRNA has been found in vascular smooth muscle cells (VSMCs) of arteries and capillaries, as well as in a subset of VSMCs of human atherosclerotic plaques. This study investigated the developmental expression of AQP1 in the chick CAM by Western blot and immunohistochemistry. Western blot results show that a major nonglycosylated band was observed with electrophoretic mobility of approximately 28 kDa in the three developmental stages examined. Immunohistochemistry data demonstrate that AQP1 was clearly expressed in the ectodermal and endodermal epithelia, the vascular endothelium, and the VSMCs. Because little information is available on the behavior of microvessel AQP1 during angiogenesis in normal and pathological conditions, our data relative to the pattern of expression of AQP1 in CAM blood vessels in normal conditions may be considered a useful tool to further investigate its modifications in several experimental conditions implying a stimulation or an inhibition of angiogenesis in the CAM assay. Anat Rec 268:85–89, 2002. © 2002 Wiley-Liss, Inc.

  • Chorioallantoic Membrane capillary bed: a useful target for studying angiogenesis and anti-angiogenesis in vivo.
    The Anatomical record, 2001
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Luisa Roncali, Peter H. Burri, Valentin Djonov
    Abstract:

    The chick embryo Chorioallantoic Membrane (CAM) is an extraembryonic Membrane that is commonly used in vivo to study both angiogenesis and anti-angiogenesis. This review 1) summarizes the current knowledge about the structure of the CAM's capillary bed; 2) discusses the controversy about the existence of a single blood sinus or a capillary plexus underlying the chorionic epithelium; 3) describes a new model of the CAM vascular growth, namely the intussusceptive mode; 4) reports findings regarding the role played by endogenous fibroblast growth factor-2 in CAM vascularization; and 5) addresses the use and limitations of the CAM as a model for studying angiogenesis and anti-angiogenesis.

  • mast cells and their secretory granules are angiogenic in the chick embryo Chorioallantoic Membrane
    Clinical & Experimental Allergy, 2001
    Co-Authors: Domenico Ribatti, Beatrice Nico, Angelo Vacca, Luisa Roncali, Enrico Crivellato, Luigi Candussio, F Dammacco
    Abstract:

    Background Many data suggest that the density of mast cells is highly correlated with the extent of both normal and pathological angiogenesis. Objective In this study we have compared in an in vivo assay, the chick embryo Chorioallantoic Membrane, the angiogenic potential of mast cell suspensions isolated from rats, degranulated mast cells and their secretory granules. Methods Gelatin sponges adsorbed with cell suspensions of rat mast cells, degranulated mast cells and their secretory granules were implanted on the top of the Chorioallantoic Membrane at day 8 of incubation. At day 12 the angiogenic response was evaluated macroscopically, microscopically and by a morphometric method of ‘point counting’. Results Isolated mast cells and their secretory granules, but not degranulated mast cells, induced an angiogenic response in the Chorioallantoic Membrane. The addition of antifibroblast growth factor-2 or antivascular endothelial growth factor antibodies reduced the angiogenic response of both mast cells and their secretory granules by 50% and 30%, respectively. Conclusion These data support the evidence that the angiogenic properties of mast cells depend on the angiogenic molecules contained in their secretory granules and indicate that fibroblast growth factor-2 and vascular endothelial growth factor are the angiogenic cytokines primarily and perhaps synergistically responsible for this vasoproliferative activity.