The Experts below are selected from a list of 1533 Experts worldwide ranked by ideXlab platform
Kebede Deribe - One of the best experts on this subject based on the ideXlab platform.
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validation of loop mediated isothermal amplification for the detection of loa loa infection in Chrysops spp in experimental and natural field conditions
Parasites & Vectors, 2021Co-Authors: Glory Ngongeh Amambo, Mathias E Esum, Abdel Jelil Njouendou, Fanny F. Fombad, Raphael Awah Abong, Franck Noel Nietcho, Amuam Andrew Beng, Ritter Manuel, Kebede DeribeAbstract:The mass drug administration of ivermectin for onchocerciasis control has contributed to a significant drop in Loa loa microfilaria loads in humans that has, in turn, led to reduction of infection levels in Chrysops vectors. Accurate parasite detection is essential for assessing loiasis transmission as it provides a potential alternative or indirect strategy for addressing the problem of co-endemic loiasis and lymphatic filariasis through the Onchocerciasis Elimination Programme and it further reflects the true magnitude of the loiasis problem as excess human mortality has been reported to be associated with the disease. Although microscopy is the gold standard for detecting the infection, the sensitivity of this method is compromised when the intensity of infection is low. The loop-mediated isothermal amplification (LAMP) assay of parasite DNA is an alternative method for detecting infection which offers operational simplicity, rapidity and versatility of visual readout options. The aim of this study was to validate the Loa loa LAMP assay for the detection of infected Chrysops spp. under experimental and natural field conditions. Two sets of 18 flies were fed on volunteers with either a low ( 30,000mf/ml) microfilarial load. The fed flies were maintained under laboratory conditions for 14 days and then analysed using LAMP for the detection of L. loa infection. In addition, a total of 9270 flies were collected from the north-west, east, and south-west regions (SW 1 and 2) of Cameroon using sweep nets and subjected to microscopy (7841 flies) and LAMP (1291 flies plus 138 nulliparous flies) analyses. The LAMP assay successfully detected parasites in Chrysops fed on volunteers with both low and high microfilariaemic loads. Field validation and surveillance studies revealed LAMP-based infection rates ranging from 0.5 to 31.6%, with the lowest levels in SW 2 and the highest infection rates in SW 1. The LAMP assay detected significantly higher infection rates than microscopy in four of the five study sites. This study demonstrated the potential of LAMP as a simple surveillance tool. It was found to be more sensitive than microscopy for the detection of experimental and natural L. loa infections in Chrysops vectors.
Waessle, Juan A. - One of the best experts on this subject based on the ideXlab platform.
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Extensión del límite austral de distribución de tres especies de peces óseos tropicales en la costa de la Provincia de Buenos Aires, Argentina
Pontificia Universidad Católica de Valparaíso, 2012Co-Authors: Milessi, Andres Conrado, Colonello, Jorge H., Cortés Federico, Lasta, Carlos Ángel, Waessle, Juan A., Allega LucreciaAbstract:Se presentan tres nuevos registros de especies de peces óseos cuya distribución corresponde a aguas tropicales, capturados por buques pesqueros en la costa de la Provincia de Buenos Aires, Argentina (CPBA, 36º-38ºS). Los ejemplares corresponden a las especies: Rachycentrum canadum, Elops saurus y Caulolatilus Chrysops. Estos registros amplían significativamente el límite austral de su distribución. La presencia ocasional de estas especies está asociada al transporte de agua subtropical cálida proveniente de la plataforma continental brasilera. Esta hipótesis es probada mediante el análisis de imágenes satelitales de temperatura superficial del mar.We document the southern records of three species of tropical bony fishes along the coast of Buenos Aires Province, Argentina (CBAP, 36-38ºS). Rachycentrum canadum, Elops saurus and Caulolatilus Chrysops were caught by bottom-trawl commercial vessels. Records presented here significantly extend southward their latitudinal distribution from those previously reported. Using sea surface temperature satellite images we show a southward warm circulation event from Brazil as a putative factor to explain the occurrence of these bony fishes in the CBAP
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Extensión del límite austral de distribución de tres especies de peces óseos tropicales en la costa de la Provincia de Buenos Aires, Argentina
Latin american journal of aquatic research, 2012Co-Authors: Milessi,andrés C, Colonello, Jorge H., Lasta,carlos A, Waessle, Juan A.Abstract:Se presentan tres nuevos registros de especies de peces óseos cuya distribución corresponde a aguas tropicales, capturados por buques pesqueros en la costa de la Provincia de Buenos Aires, Argentina (CPBA, 36°-38°S). Los ejemplares corresponden a las especies: Rachycentrum canadum, Elops saurus y Caulolatilus Chrysops. Estos registros amplían significativamente el límite austral de su distribución. La presencia ocasional de estas especies está asociada al transporte de agua subtropical cálida proveniente de la plataforma continental brasilera. Esta hipótesis es probada mediante el análisis de imágenes satelitales de temperatura superficial del mar
M. E. Westerman - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterization of 149 novel microsatellite DNA markers for striped bass, Morone saxatilis, and cross-species amplification in white bass, Morone Chrysops, and their hybrid
Molecular Ecology Notes, 2006Co-Authors: Charlene R. Couch, Amber F. Garber, Caird E. Rexroad, J. M. Abrams, J. A. Stannard, M. E. WestermanAbstract:To support detailed genetic analysis of striped bass ( Morone saxatilis ) and white bass ( Morone Chrysops ), we isolated 153 microsatellite loci from repeat-enriched striped bass DNA libraries. Of these, 147 markers amplified in striped bass (average 4.7 alleles per locus) and 133 in white bass (average 2.2 alleles per locus). One hundred twenty-two markers amplified in their hybrid. Development of new microsatellite markers will facilitate evaluations of genetic structure in wild populations and will support pedigree analysis and linkage mapping for selective breeding.
Carl D Webster - One of the best experts on this subject based on the ideXlab platform.
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differential susceptibility of white bass morone Chrysops striped bass morone saxatilis and hybrid striped bass m Chrysops m saxatilis to flavobacterium columnare and effects of mucus on bacterial growth and biofilm development
Journal of Fish Diseases, 2021Co-Authors: Bradley D Farmer, Sidney Adam Fuller, Benjamin H Beck, Jason Abernathy, Miles D Lange, Carl D WebsterAbstract:Columnaris disease generates substantial losses of many freshwater fish species; one is the hybrid striped bass. The ubiquitous aquatic bacterium Flavobacterium columnare can be highly effective in biofilm formation on fish skin and gills. Previous research showed a difference between columnaris disease susceptibility of hybrid striped bass (Morone saxatilis × M. Chrysops) and white bass (M. Chrysops). To understand these differential susceptibilities and possible mucosal relationship, we assessed total bacterial growth and biofilm formation with mucus derived from each moronid parental species: white bass and striped bass (M. saxatilis). Differential susceptibility was confirmed of the other parent species, the striped bass (M. saxatilis). In addition to intraspecies investigations, individual hybrid striped bass mucosal affects were also studied for deferential responses to bacterial growth and biofilm formation. Species- and concentration-dependent differences were detected in the total growth of the bacteria to host mucus. Our data suggest that bass mucus can significantly affect biofilm formation with the F. columnare isolate tested. There appears to be a correlation between the bacteria's response of growth and biofilms and bass species susceptibility. This study provides insight into our understanding of the host-pathogen interaction between F. columnare and moronids.
John R Gold - One of the best experts on this subject based on the ideXlab platform.
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microsatellite dna markers for parental assignment in hybrid striped bass morone saxatilis morone Chrysops
Molecular Ecology Notes, 2004Co-Authors: Kirstin Ross, Xiaoxue Wang, Kathleen G Omalley, John R GoldAbstract:Development of nine polymorphic microsatellites from a genomic library of hybrid striped bass (female Morone Chrysops × × × male Morone saxatilus ) DNA is described. Breeding of hybrid striped bass for aquaculture is based largely on breeding wild fish. Molecular markers such as microsatellites will be useful tools for developing broodstock, estimating heritability for production traits, and selective breeding via marker-assisted selection. The nine polymorphic microsatellites include six dinucleotide and three complex repeat motifs. The number of alleles detected among a sample of 10 individuals of each species was relatively low. All polymerase chain reaction primer pairs also amplified products in the sea bass Dicentrarchus labrax .