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Adil Denizli - One of the best experts on this subject based on the ideXlab platform.
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Cibacron Blue F3ga modified disposable pencil graphite electrode for the investigation of affinity binding to bovine serum albumin
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Filiz Kuralay, Lokman Uzun, Erkut Yilmaz, Adil DenizliAbstract:Abstract In this work, Cibacron Blue F3ga (CB) modified pencil graphite electrodes (PGEs) were prepared and their affinities to bovine serum albumin were investigated. Preparation of the PGEs was performed using cyclic voltammetry (CV) and passive adsorption techniques. Improved electrochemical results were obtained with the PGEs prepared by CV technique compared to the PGEs prepared by passive adsorption technique. In order to obtain more sensitive results number of scans used in CV technique and the effect of concentration of CB were studied. Scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS) were used for the characterization of modified electrodes. The modified PGEs were then used for the electrochemical monitoring of affinity interaction between CB and bovine serum albumin. The effect of BSA concentration and interfering species (tryptophan, glucose and immunoglobulin G) on the response of the electrode were examined. The aim of this study was to prepare an easy, fast, stable and cheap modified electrode for the investigation of the well-known affinity of CB to serum albumin. The electrochemistry can provide alternative routes for dye–protein interaction instead of using classical time-consuming methods.
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evaluation of human interferon adsorption performance of Cibacron Blue F3ga attached cryogels and interferon purification by using fplc system
Journal of Chromatography B, 2012Co-Authors: Ali Dogan, Serpil Ozkara, Mufrettin Murat Sari, Lokman Uzun, Adil DenizliAbstract:Abstract In this study, we have focused our attention on preparing supermacroporous cryogels as a potential dye-affinity adsorbent for interferon purification. For this purpose, 2-hydroxyethyl methacrylate (HEMA) and Cibacron Blue F3ga (CB) were selected as main monomer and dye–ligand. Cibacron Blue F3ga attached supermacroporous poly(2-hydroxyethyl methacrylate) [poly(HEMA)/CB] cryogels were prepared and characterized by swelling test, scanning electron microscopy, elemental analysis, and FTIR. After that, the effecting factors such as pH, concentration, interaction time, and ionic strength on the interferon separation were evaluated. The maximum adsorption capacity of poly(HEMA)/CB cryogels was obtained as 38.2 mg/g at pH 6.0. Fast protein liquid chromatography (FPLC) system was used for interferon purification from human gingival fibroblast extract. The chromatography parameters, capacity and selectivity factors, resolution and theoretical plate number were found as 7.79, 9.62, 4.23 and 554, respectively. Although some decreases in total protein content, from 320 μg to 18 μg, and interferon activity, from 2.6 × 10 3 IU to 2.2 × 10 3 IU, were determined, specific antiviral activity increased from 7.19 IU/μg to 122.2 IU/μg. The purified interferon samples have 97.6% purity determined by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. After repeated ten adsorption–desorption cycles, no significant decrease was determined in adsorption capacity of cryogel. In result, poly(HEMA)/CB cryogels have an application potential for rapid, cheap and specific purification of interferon.
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predicting the binding properties of Cibacron Blue F3ga in affinity separation systems
International Journal of Biological Macromolecules, 2007Co-Authors: Cenk A Andac, Muge Andac, Adil DenizliAbstract:Abstract The binding properties of Cibacron Blue F3ga (CB-F3ga) bound to a model NAD(P)H/FAD(H 2 )-dependent protein system, namely cytosolic quinone reductase (QR), was characterized by AMBER in an attempt to address the binding properties of immobilized CB-F3ga used in the separation of serum albumin. A favorable binding free energy of −4.52 kcal/mol ( K D = 5.09 × 10 −4 kcal/mol) was determined for CB-F3ga binding by MM-PBSA method, which was found to be a ballpark estimate of empirical values reported in literature (Δ G ≈ −6 kcal/mol). We propose that CB-F3ga primarily follows a class III binding motif in presence of FAD in the binding site of QR in solution, while a class II binding motif is observed in the crystal form. It was found that favorable van der Waals/hydrophobic interactions take place in the binding site making a major contribution to a favorably dominating enthalpy of binding (Δ H tot = −25.87 kcal/mol) as compared to a disfavorable binding entropy term ( T Δ S tot = −21.35 kcal/mol). Additional MM-PBSA experiments in the absence of FAD gave rise to a disfavorable binding free energy for CB in complex with QR, suggesting that FAD is an essential determinant of CB-F3ga binding. This is in contrast to an earlier observation of Denizli et al. on separation of human serum albumin (HSA) by immobilized CB-F3ga in the absence of FAD. Therefore, a class I binding model for CB-F3ga is proposed here to account for the efficient separation of HSA in affinity chromatography systems.
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Cibacron Blue F3ga incorporated magnetic poly 2 hydroxyethyl methacrylate beads for lysozyme adsorption
Journal of Applied Polymer Science, 2004Co-Authors: Mehmet Odabasi, Adil DenizliAbstract:Lysozyme adsorption onto Cibacron Blue F3ga incorporated magnetic poly(2-hydroxyethyl methacrylate) (mag-PHEMA) beads was investigated. Mag-PHEMA beads (80–120 μm) were produced by dispersion polymerization. The triazine dye Cibacron Blue F3ga was incorporated covalently as a dye ligand. These dye-incorporated beads, having a swelling ratio of 34% (w/w) and carrying different amounts of Cibacron Blue F3ga (12.2–42.8 μmol/g) were used in lysozyme adsorption studies. The effects of the initial concentration, pH, ionic strength, and temperature on the adsorption efficiency of dye-incorporated beads were studied in a batch reactor. The effects of the Cibacron Blue F3ga loading on the lysozyme adsorption were also studied. The nonspecific adsorption of lysozyme on the mag-PHEMA beads was 0.8 mg/g. Cibacron Blue F3ga attachment significantly increased the lysozyme adsorption up to 295 mg/g. More than 90% of the adsorbed lysozyme was desorbed in 1 h in a desorption medium containing 1.0M KSCN at pH 8.0. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 93: 719–725, 2004
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Cibacron Blue F3ga attached magnetic poly 2 hydroxyethyl methacrylate beads for human serum albumin adsorption
Polymer International, 2004Co-Authors: Mehmet Odabasi, Adil DenizliAbstract:An affinity dye ligand, Cibacron Blue F3ga, was covalently attached onto magnetic poly(2-hydroxyethyl methacrylate) (mPHEMA) beads for human serum albumin (HSA) adsorption from both aqueous solutions and human plasma. The mPHEMA beads, in the size range of 80 to 120 µm, were prepared by a modified suspension technique. Cibacron Blue F3ga molecules were incorporated on to the mPHEMA beads. The maximum amount of Cibacron Blue F3ga attachment was obtained as 68.3 µmol g−1. HSA adsorption onto unmodified and Cibacron Blue F3ga-attached mPHEMA beads was investigated batchwise. The non-specific adsorption of HSA was very low (1.8 mg g−1). Cibacron Blue F3ga attachment onto the beads significantly increased the HSA adsorption (94.5 mg g−1). The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma (138.3 mg HSA g−1). Desorption of HSA from Cibacron Blue F3ga-attached mPHEMA beads was obtained by using 0.1 M Tris/HCl buffer containing 0.5 M NaSCN. High desorption ratios (up to 98% of the adsorbed HSA) were observed. It was possible to re-use Cibacron Blue F3ga-attached mPHEMA beads without any significant decreases in their adsorption capacities. Copyright © 2004 Society of Chemical Industry
Erhan Piskin - One of the best experts on this subject based on the ideXlab platform.
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albumin adsorption from aqueous solutions and human plasma in a packed bed column with Cibacron Blue F3ga zn ii attached poly egdma hema microbeads
Reactive & Functional Polymers, 1999Co-Authors: Adil Denizli, Handan Yavuz, Gulay Kokturk, Erhan PiskinAbstract:Affinity dye-ligand Cibacron Blue F3ga, was covalently coupled with poly(EGDMA-HEMA) microbeads via nucleophilic reaction between the chloride of its triazine ring and the hydroxyl groups of the HEMA under alkaline conditions. The microbeads carrying 16.5 μmol Cibacron Blue F3ga per gram polymer was incorporated with Zn(II) ions. Zn(II) loading was 189.6 μmol/g. Cibacron Blue F3ga-Zn(II) attached affinity sorbent was used for albumin adsorption from aqueous solutions and human plasma in a packed-bed column. BSA adsorption capacity of the microbeads decreased with an increase in the recirculation rate. High adsorption rates were observed at the beginning, then equilibrium was gradually achieved in about 60 min. The BSA concentration in the mobile phase also effected adsorption. BSA adsorption was first increased with BSA concentration, then reached a plateau which was about 128 mg BSA/g. The maximum adsorption was observed at pH 5.0 which is the isoelectric pH of BSA. Higher human serum albumin adsorption was observed from human plasma (215 mg HSA/g). High desorption ratios (over 90% of the adsorbed albumin) were achieved by using 1.0 M NaSCN (pH 8.0) in 30 min.
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cadmium removal from human plasma by Cibacron Blue F3ga and thionein incorporated into polymeric microspheres
Journal of Chromatography B: Biomedical Sciences and Applications, 1998Co-Authors: Elhadi Ibrahim, Adil Denizli, Sema Bektas, Omer Genc, Erhan PiskinAbstract:Abstract Poly(2-hydroxyethylmethacrylate–ethyleneglycoldimethacrylate) [poly(HEMA–EGDMA)] microspheres carrying Cibacron Blue F3ga and/or thionein were prepared and used for the removal of cadmium ions Cd(II) from human plasma. The poly(HEMA–EGDMA) microspheres, in the size range of 150–200 μm in diameter, were produced by a modified suspension copolymerization of HEMA and EGDMA. The reactive triazinyl dye-ligand Cibacron Blue F3ga was then covalently incorporated into the microspheres. The maximum dye incorporation was 16.5 μmol/g. Then, thionein was bound onto the Cibacron Blue F3ga-incorporated microspheres under different conditions. The maximum amount of thionein bound was 14.3 mg/g. The maximum amounts of Cd(II) ions removed from human plasma by poly(HEMA–EGDMA)–Cibacron Blue F3ga and poly(HEMA–EGDMA)–Cibacron Blue F3ga–thionein were of 17.5 mg/g and 38.0 mg/g, respectively. Cd(II) ions could be repeatedly adsorbed and desorbed with both types of microspheres without significant loss in their adsorption capacity.
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adsorption of heavy metal ions on Cibacron Blue F3ga immobilized microporous polyvinylbutyral based affinity membranes
Journal of Membrane Science, 1997Co-Authors: Adil Denizli, Bekir Salih, Deniz Tanyolac, Emel Aydinlar, Ahmet R Ozdural, Erhan PiskinAbstract:Abstract Microporous polyvinylbutyral membranes were prepared by a solvent-casting technique. An affinity dye, i.e. Cibacron Blue F3ga was then immobilized. These affinity membranes with a high water content of 89%, and carrying 3.3 mmol Cibacron Blue F3ga/m 2 membrane were used in the adsorption/stripping of some selected heavy-metal ions (e.g. Cu(II), Zn(II), Cd(II) and Pb(II) from aqueous media containing different amounts of these ions (0.024–4.60 mmol/1) and at different pH values (2.0–9.0). Adsorption rates were very high, and adsorption equilibria were reached in ca. 15 min. The maximum adsorptions of heavy-metal ions onto the Cibacron Blue F3ga-immobilized affinity membranes from their single solutions were 7.0 mmol/m 2 for Cu(II), 16.8 mmol/m 2 for Zn(II), 22.2 mmol/m 2 for Cd(II), and 34.2 mmol/m 2 for Pb(II). Similar behavior was observed when the heavy-metal ions competed (in the case of the adsorption from their mixture), the order of affinity was Pb(II)> Cd(II)> Zn(II)> Cu(II). Heavy-metal ions regeneration was achieved by using 0.1 M HNO 3 in 30 min. It was observed that heavy-metal ions could be repeatedly adsorbed and tripped without significant loss in adsorption capacity.
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Cibacron Blue F3ga and zn ii containing poly ethylene glycol dimethacrylate hydroxyethyl methacrylate microbeads for albumin adsorption
Journal of Macromolecular Science Part A, 1997Co-Authors: Adil Denizli, Bekir Salih, Erhan PiskinAbstract:Abstract Swellable poly (ethylene glycol dimethacrylate-hydroxyethyl methacryate) [poly(EGDMA-HEMA] microbeads, in the size range of 150–200 μm, were produced by a modified suspension copolymerization of EGDMA and HEMA. Cibacron Blue F3ga was attached, then Zn(II) ions were incorporated within the microbeads by chelating with the Cibacron Blue F3ga molecules. The maximum amount of Cibacron Blue F3ga attachment was 16.5 μmol/g polymer. Different amounts of Zn(II) ions (0.4–32.4 mg/g polymer were incorporated on the microbeads by changing the initial concentration of Zn(II) ions and medium pH. Bovine serum albumin (BSA) adsorption onto unmodified, Cibacron Blue F3ga-attached and Cibacron Blue F3ga-Zn(II)-derivatized microbeads was investigated. The non-specific adsorption of BSA was very low (0.051 mg/g polymer). Cibacron Blue F3ga attachment significantly increased the BSA adsorption (60.5 mg/g polymer). The maximum BSA adsorption (122 mg/g polymer) was observed at pH 6.0 when Cibacron Blue F3ga-Zn(II)-der...
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catalase adsorption onto Cibacron Blue F3ga and fe iii derivatized poly hydroxyethyl methacrylate membranes and application to a continuous system
Journal of Membrane Science, 1997Co-Authors: Yakup M Arica, Adil Denizli, Bekir Salih, Erhan Piskin, Vasif HasirciAbstract:Poly(2-hydroxyethyl methacrylate) (poly(HEMA)) membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (a-a′-azobisisobutyronitrile, AIBN). An affinity dye, i.e. Cibacron Blue F3ga (CB) was incorporated covalently and then complexed with Fe(III) ions. The polyHEMA-CB and polyHEMA-CB-Fe(III) derivatized membranes were used in the adsorption of catalase (CAT). The enzyme-loading capability of the Fe(III)-containing membrane (23.6 μg/cm2) was greater than that of the poly(HEMA)-CB derivatized membrane (17.1 μg/cm2). The adsorption phenomena appeared to follow a typical Langmuir isotherm. The Km values for both immobilized catalases (poly(HEMA)-CB-CAT (22.4 mM) and poly(HEMA)-CB-Fe(III)-CAT (19.3 mM)) were higher than that of free enzyme (16.5 mM). Optimum operational temperature was 5°C higher than that of the free enzyme and was significantly broader. A similar observation was made for the optimum pH. Operational, thermal and storage stabilities were found to increase with immobilization, especially in the presence of Fe(III). It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity or enzyme activity.
Yakup M Arica - One of the best experts on this subject based on the ideXlab platform.
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zinc ion promoted adsorption of lysozyme to Cibacron Blue F3ga attached microporous polyamide hollow fiber membranes
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2001Co-Authors: Serap şenel, Yakup M Arica, Adil DenizliAbstract:Abstract Dye-affinity and metal chelate affinity adsorption are increasingly used for protein separation. Synthetic hollow fiber membranes have advantages as support matrices in comparison to conventional bead supports because they are not compressible and they eliminate internal diffusion limitations. The goal of this study was to explore in detail the performance of hollow fibers composed of modified polyamide to which Cibacron Blue F3ga and Zn(II) were attached for adsorption of lysozyme. The polymer matrix was characterized by scanning electron microscopy. These dye-affinity and Zn(II) chelated hollow-fibers were used in the lysozyme adsorption–elution studies. The effects of initial concentration of lysozyme and medium pH on the adsorption efficiency of dye-attached and metal-chelated hollow-fibers were studied in a batch reactor. The effect of Zn(II) loading on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the polyamide hollow-fibers was 1.8 mg g −1 . Cibacron Blue F3ga attachment significantly increased the lysozyme adsorption up to 63.2 mg g −1 . Lysozyme adsorption capacity of the Zn(II) chelated hollow-fibers (144.2 mg g −1 ) was greater than that of the Cibacron Blue F3ga-attached hollow-fibers. A significant amount of the adsorbed lysozyme (up to 97%) was eluted in 1 h in the elution medium containing 1.0 M NaSCN at pH 8.0 and 25 mM EDTA at pH 4.9. In order to examine the effects of separation conditions on possible conformational changes of lysozyme structure, fluorescence spectrophotometry was employed. We conclude that dye- and metal-chelate affinity chromatography with polyamide hollow-fibers can be applied for lysozyme adsorption without causing any significant conformational changes and denaturation. Repeated adsorption/elution processes showed that these novel dye-attached and Zn(II) chelated hollow-fibers are suitable for lysozyme adsorption.
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Removal of cadmium(II) ions from human plasma by thionein modified pHEMA based membranes
Reactive & Functional Polymers, 2000Co-Authors: Fatma Denizli, Yakup M Arica, Adil DenizliAbstract:Abstract Microporous poly(2-hydroxyethylmethacrylate) [pHEMA] membranes carrying Cibacron Blue F3ga and/or thionein were prepared and used for cadmium(II) removal from human plasma. pHEMA membranes were produced by a photopolymerization of HEMA in the presence of azobisisobutyronitrile. The reactive dye-ligand Cibacron Blue F3ga was then covalently attached onto the pHEMA membranes. The maximum dye-loading was 1.07 μmol/cm 2 . Then, thionein was adsorbed onto the Cibacron Blue F3ga-carrying membranes. The maximum amount of thionein adsorbed was 0.92 μmol/cm 2 . The aqueous swelling properties and contact angle (air underwater) of the pHEMA membrane were not observed to change following derivatisation with Cibacron Blue F3ga. These hydrophilic membranes (contact angle value: 45.3°) with a swelling ratio of 58% (w/w), and carrying Cibacron Blue F3ga and/or thionein were used in the Cd(II) removal studies. The maximum amounts of Cd(II) removed from human plasma by Cibacron Blue F3ga-attached and Cibacron Blue F3ga-attached/thionein adsorbed pHEMA membranes were of 1.72 μmol used as pectively. Cd(II) ions could be repeatedly adsorbed and desorbed with both types of these novel metallo–peptide affinity pHEMA membranes without noticeable loss in their Cd(II) adsorption capacity.
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adsorption of heavy metal ions onto ethylene diamine derived and Cibacron Blue F3ga incorporated microporous poly 2 hydroxyethyl methacrylate membranes
Reactive & Functional Polymers, 2000Co-Authors: Adil Denizli, Suleyman Patir, Yakup M AricaAbstract:Abstract Microporous poly(2-hydroxyethylmethacrylate) (PHEMA) membranes carrying ethylene diamine (EDA) and Cibacron Blue F3ga were prepared for the removal of heavy metal ions (i.e. mercury, copper, lead and cadmium) from aqueous solutions containing different amounts of these ions (5–700 μg l −1 ) and at different pH values (2.0–8.0). The non-specific adsorption of heavy metal ions on the underived membranes was very low (3.3 mmol/m 2 for Hg(II), 0.5 mmol/m 2 for Cu(II), 1.2 mmol/m 2 for Pb(II) and 1.1 mmol/m 2 for Cd(II)). Cibacron Blue F3ga attachment significantly increased the heavy metal adsorption (16.3 mmol/m 2 for Hg(II), 19.9 mmol/m 2 for Cu(II), 23.4 mmol/m 2 for Pb(II) and 38.4 mmol/m 2 for Cd(II)). When the heavy metal ions competed (in the case of the adsorption from a mixture) the adsorption capacities were 7.1 mmol/m 2 for Hg(II), 17.9 mmol/m 2 for Cu(II), 6.2 mmol/m 2 for Pb(II) and 9.1 mmol/m 2 for Cd(II). The observed order in adsorption was found to be Cd(II)>Pb(II)>Cu(II)>Hg(II) for non-competitive conditions. The adsorption of heavy metal ions increased with increasing pH and reached a plateau value at around pH 5.0. Desorption of heavy metal ions was achieved using 0.1 M HNO 3 . These membranes are suitable for repeated use for more than five cycles without considerable loss of adsorption capacity.
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affinity microspheres and their application to lysozyme adsorption Cibacron Blue F3ga and cu ii with poly hema egdma
Polymer International, 1999Co-Authors: Fatma Denizli, Adil Denizli, Yakup M AricaAbstract:Lysozyme adsorption onto Cibacron Blue F3ga attached and Cu(II) incorporated poly(2-hydroxyethyl methacrylate-ethylene glycol dimethacrylate) [poly(HEMA-EGDMA)] microspheres was investigated. The microspheres were prepared by suspension polymerization. Various amounts of Cibacron Blue F3ga were attached covalently onto the microspheres by changing the initial concentration of dye in the reaction medium. The microspheres with a swelling ratio of 65%, and carrying different amounts of dye (between 1.4 and 22.5 μmol/g -1 ) were used in the lysozyme adsorption studies. Lysozyme adsorption on these microspheres from aqueous solutions containing different amounts of lysozyme at different pH values was investigated in batch reactors. The lysozyme adsorption capacity of the dye-metal chelated microspheres (238.2 mg g -1 ) was greater than that of the dye-attached microspheres (175.1 mg g -1 ). The maximum lyzozyme adsorption capacities (q m and the dissociation constant (k d ) values were found to be 204.9 mg g -1 and 0.0715 mg ml -1 with dye-attached and 270.7 mg g -1 and 0.0583 mg ml -1 with dye-metal chelated microspheres, respectively. More than 90% of the adsorbed lysozyme were desorbed in 60min in the desorption medium containing 0.5M KSCN at pH 8.0 or 25 mM EDTA at pH 4.9.
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Cibacron Blue F3ga and cu ii derived poly 2 hydroxyethylmethacrylate membranes for lysozyme adsorption
Colloids and Surfaces B: Biointerfaces, 1998Co-Authors: Adil Denizli, Serap şenel, Yakup M AricaAbstract:Abstract Lysozyme adsorption onto Cibacron Blue F3ga and Cu(II) derived poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes was investigated. Microporous poly(HEMA) membranes were prepared by photopolymerization of HEMA. The triazine dye, Cibacron Blue F3ga was attached covalently as a dye-ligand. These dye-membranes with a swelling ratio of 58% (w/w), and carrying different amounts of Cibacron Blue F3ga (between 0.35 and 1.07 μmol cm −2 ) were used in the lysozyme adsorption studies. The effect of initial concentration and pH on the adsorption efficiency of dye-derived and metal-chelated membranes were studied in a batch reactor. The effect of Cu(II) incorporation on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the poly(HEMA) membranes was 0.9 μg cm −2 . Cibacron Blue F3ga attachment significantly increased the lysozyme adsorption up to 133.3 μg cm −2 . Lysozyme adsorption capacity of the Cu(II) incorporated membranes (165.1 μg cm −2 ) was greater than that of the Cibacron Blue F3ga-attached membranes. More than 85% of the adsorbed lysozyme was desorbed in 1 h in the desorption medium containing 0.5 M potassium thiocyanate (KSCN) at pH 8.0.
Bekir Salih - One of the best experts on this subject based on the ideXlab platform.
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metal chelating properties of Cibacron Blue F3ga derived poly egdma hema microbeads
Journal of Applied Polymer Science, 1999Co-Authors: Adil Denizli, Bekir Salih, Kemal Kesenci, Serap EnelAbstract:Metal chelating properties of Cibacron Blue F3ga-derived poly(EGDMA-HEMA) microbeads have been studied. Poly(EGDMA-HEMA) microbeads were prepared by suspension copolymerization of ethylene glycol dimethacrylate (EGDMA) and hydroxy-ethyl methacrylate (HEMA) by using poly(vinyl alcohol), benzoyl peroxide, and toluene as the stabilizer, the initiator, and the pore-former, respectively. Cibacron Blue F3ga was covalently attached to the microbeads via the nucleophilic substitution reaction between the chloride of its triazine ring and the hydroxyl groups of the HEMA, under alkaline conditions. Microbeads (150-200 μm in diameter) with a swelling ratio of 55%, and carrying 16.5 μmol Cibacron Blue F3ga/g polymer were used in the adsorption/desorption studies. Adsorption capacity of the microbeads for the selected metal ions, i.e., Cu(II), Zn(II), Cd(II), Fe(III), and Pb(II) were investigated in aqueous media containing different amounts of these ions (5-200 ppm) and at different pH values (2.0-7.0). The maximum adsorptions of metal ions onto the Cibacron Blue F3ga-derived microbeads were 0.19 mmol/g for Cu(II), 0.34 mmol/g for Zn(II), 0.40 mmol/g for Cd(II), 0.91 mmol/g for Fe(III), and 1.05 mmol/g for Pb(II). Desorption of metal ions were studied by using 0.1 M HNO 3 . High desorption ratios (up to 97%) were observed in all cases. Repeated adsorption/desorption operations showed the feasibility of repeated use of this novel sorbent system.
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Metal chelating properties of Cibacron Blue F3ga‐derived poly(EGDMA‐HEMA) microbeads
Journal of Applied Polymer Science, 1999Co-Authors: Adil Denizli, Bekir Salih, Kemal Kesenci, Serap EnelAbstract:Metal chelating properties of Cibacron Blue F3ga-derived poly(EGDMA-HEMA) microbeads have been studied. Poly(EGDMA-HEMA) microbeads were prepared by suspension copolymerization of ethylene glycol dimethacrylate (EGDMA) and hydroxy-ethyl methacrylate (HEMA) by using poly(vinyl alcohol), benzoyl peroxide, and toluene as the stabilizer, the initiator, and the pore-former, respectively. Cibacron Blue F3ga was covalently attached to the microbeads via the nucleophilic substitution reaction between the chloride of its triazine ring and the hydroxyl groups of the HEMA, under alkaline conditions. Microbeads (150-200 μm in diameter) with a swelling ratio of 55%, and carrying 16.5 μmol Cibacron Blue F3ga/g polymer were used in the adsorption/desorption studies. Adsorption capacity of the microbeads for the selected metal ions, i.e., Cu(II), Zn(II), Cd(II), Fe(III), and Pb(II) were investigated in aqueous media containing different amounts of these ions (5-200 ppm) and at different pH values (2.0-7.0). The maximum adsorptions of metal ions onto the Cibacron Blue F3ga-derived microbeads were 0.19 mmol/g for Cu(II), 0.34 mmol/g for Zn(II), 0.40 mmol/g for Cd(II), 0.91 mmol/g for Fe(III), and 1.05 mmol/g for Pb(II). Desorption of metal ions were studied by using 0.1 M HNO 3 . High desorption ratios (up to 97%) were observed in all cases. Repeated adsorption/desorption operations showed the feasibility of repeated use of this novel sorbent system.
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new metal chelate sorbent for albumin adsorption Cibacron Blue F3ga zn ii attached microporous poly hema membranes
Journal of Applied Polymer Science, 1998Co-Authors: Adil Denizli, Bekir Salih, Serap şenel, Yakup M AricaAbstract:Poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (α-α′-azobis-isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3ga was attached as an affinity ligand to poly(HEMA) membranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3ga/m2 were used in the albumin adsorption studies. After dye-attachment, Zn(II) ions were chelated within the membranes via attached-dye molecules. Different amounts of Zn(II) ions [650–1440 mg Zn(II)/m2] were loaded on the membranes by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these membranes from aqueous solutions containing different amounts of BSA at different pH was investigated in batch reactors. The nonspecific adsorption of BSA on the poly(HEMA) membranes was negligible. Cibacron Blue F3ga attachment significantly increased the BSA adsorption up to 92.1 mg BSA/m2. Adsorption capacity was further increased when Zn(II) ions were attached (up to 144.8 mg BSA m2). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5M NaSCN at pH 8.0 and 0.025M EDTA at pH 4.9. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 68: 657–664, 1998
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New metal chelate sorbent for albumin adsorption: Cibacron Blue F3ga‐Zn(II) attached microporous poly(HEMA) membranes
Journal of Applied Polymer Science, 1998Co-Authors: Adil Denizli, Bekir Salih, Serap şenel, M. Yakup AricaAbstract:Poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes were prepared by UV-initiated photopolymerization of HEMA in the presence of an initiator (α-α′-azobis-isobutyronitrile, AIBN). The triazine dye Cibacron Blue F3ga was attached as an affinity ligand to poly(HEMA) membranes, covalently. These affinity membranes with a swelling ratio of 58% and containing 10.7 mmol Cibacron Blue F3ga/m2 were used in the albumin adsorption studies. After dye-attachment, Zn(II) ions were chelated within the membranes via attached-dye molecules. Different amounts of Zn(II) ions [650–1440 mg Zn(II)/m2] were loaded on the membranes by changing the initial concentration of Zn(II) ions and pH. Bovine serum albumin (BSA) adsorption on these membranes from aqueous solutions containing different amounts of BSA at different pH was investigated in batch reactors. The nonspecific adsorption of BSA on the poly(HEMA) membranes was negligible. Cibacron Blue F3ga attachment significantly increased the BSA adsorption up to 92.1 mg BSA/m2. Adsorption capacity was further increased when Zn(II) ions were attached (up to 144.8 mg BSA m2). More than 90% of the adsorbed BSA was desorbed in 1 h in the desorption medium containing 0.5M NaSCN at pH 8.0 and 0.025M EDTA at pH 4.9. © 1998 John Wiley & Sons, Inc. J Appl Polym Sci 68: 657–664, 1998
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Cibacron Blue F3ga attached polyvinylbutyral microbeads as novel magnetic sorbents for removal of cu ii cd ii and pb ii ions
Journal of Chromatography A, 1998Co-Authors: Adil Denizli, Bekir Salih, Deniz Tanyolac, Ahmet R OzduralAbstract:Abstract Magnetic polyvinylbutyral based microbeads containing 12.4 μmol Cibacron Blue F3ga/g were used in the adsorption–desorption of some selected heavy metal ions, i.e., Cu(II), Cd(II) and Pb(II) from aqueous media containing different amounts of these ions (5–500 ppm) and at different pH values (2.0–8.0). Adsorption rates were high, and adsorption equilibria were reached in about 30 min. The maximum adsorptions of heavy metal ions onto the Cibacron Blue F3ga-attached affinity microbeads from their single solutions were 9.6 mg/g for Cu(II), 38.6 mg/g for Cd(II), 86.2 mg/g for Pb(II). When the heavy metal ions competed (in the case of the adsorption from their mixture) the amounts of adsorption were 6.5 mg/g for Cu(III), 20.3 mg/g for Cd(II) and 41.6 mg/g for Pb(II). Under non-competitive and competitive conditions the affinity order of ions was Pb(II)>Cd(II)>Cu(II). Desorption of heavy metal ions was achieved using 0.1 mol HNO3 in 30 min. It was observed that heavy metal ions could be repeatedly adsorbed and desorbed without significant loss in adsorption capacity.
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Cibacron Blue F3ga incorporated poly methylmethacrylate beads for albumin adsorption in batch system
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2003Co-Authors: Handan Yavuz, Omer Genc, Erol Duru, Adil DenizliAbstract:Abstract A new adsorbent was prepared to obtain high albumin adsorption capacity utilizing Cibacron Blue F3ga as a dye ligand. Poly(methylmethacrylate) (PMMA) beads (100–150 μm in diameter) were produced by suspension polymerization. Ester groups in the PMMA structure were converted to imine groups by reacting with poly(ethylene imine) (PEI) in the presence of NaH. Then, dye ligand, Cibacron Blue F3ga, was covalently attached onto PMMA/PEI beads for human serum albumin (HSA) adsorption from both aqueous solutions and human plasma. Cibacron Blue F3ga loading was found to be 95.8 μmol g −1 . HSA adsorption onto PMMA, PMMA/PEI and Cibacron Blue F3ga-attached PMMA/PEI beads was investigated batchwise. The adsorption of HSA onto PMMA was low (6.0 mg g −1 ). Cibaron Blue F3ga attachment onto the beads increased significantly the HSA adsorption (26.0 mg g −1 ). The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma (64.3 mg HSA g −1 ). Adsorption of other serum proteins were found to be 2.8 mg g −1 for fibrinogen and 5.3 mg g −1 for γ-globulins. The total protein adsorption was determined as 73.1 mg g −1 . Desorption of HSA was obtained using 0.5 M KSCN solution. High desorption ratios (up to 98% of the adsorbed HSA) were observed. It was possible to reuse Cibacron Blue F3ga-attached PMMA/PEI beads without significant decrease in the HSA adsorption capacity.
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glucose oxidase and catalase adsorption onto Cibacron Blue F3ga attached microporous polyamide hollow fibres
Reactive & Functional Polymers, 2003Co-Authors: Sinan Akgol, Serap şenel, Handan Yavuz, Adil DenizliAbstract:Abstract The aim of this study was to explore in detail the performance of polyamide hollow fibers to which Cibacron Blue F3ga was attached for adsorption of proteins. Model proteins were glucose oxidase, as a flavo-enzyme and contains two tightly bound flavine adenine dinucleotide cofactor, and catalase as a heme-containing metallo-enzyme. The hollow fiber structure was characterized by scanning electron microscopy. These dye-carrying hollow-fibers (35.8 μmol/g) were used in the glucose oxidase and catalase adsorption–elution studies. The non-specific adsorption values were 1.25 mg/g for glucose oxidase and 1.97 mg/g for catalase. Cibacron Blue F3ga attachment increased the adsorption capacity up to 248 mg/g. Langmuir adsorption model was found to be applicable in interpreting glucose oxidase and catalase adsorption by Cibacron Blue F3ga-attached hollow fibers. Significant amount of the adsorbed proteins (up to 97%) was eluted in 1 h in the elution medium containing 1.0 M NaSCN at pH 8.0. In order to determine the effects of adsorption and elution conditions on possible conformational changes of studied protein structures, fluorescence spectrophotometry was employed. It was concluded that polyamide dye-affinity hollow-fibers can be applied for glucose oxidase and catalase adsorption without causing any significant conformational changes. Repeated adsorption/elution processes showed that these dye-attached hollow-fibers are suitable for glucose oxidase and catalase separation.
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human serum albumin chromatography by Cibacron Blue F3ga derived microporous polyamide hollow fiber affinity membranes
Journal of Chromatography B: Biomedical Sciences and Applications, 2000Co-Authors: A Kassab, Mehmet Odabasi, Handan Yavuz, Adil DenizliAbstract:Abstract An affinity dye ligand, Cibacron Blue F3ga was covalently attached onto commercially available microporous polyamide hollow-fibre membranes for human serum albumin (HSA) adsorption from both aqueous solutions and human plasma. Different amounts of Cibacron Blue F3ga were incorporated on the polyamide hollow-fibres by changing the dye attachment conditions, i.e. initial dye concentration, addition of sodium carbonate and sodium chloride. The maximum amount of Cibacron Blue F3ga attachment was obtained at 42.5 μmol g −1 when the hollow-fibres were treated with 3 M HCl for 30 min before performing the dye attachment. HSA adsorption onto unmodified and Cibacron Blue F3ga-derived polyamide hollow-fibre membranes was investigated batchwise. The non-specific adsorption of HSA was very low (6.0 mg g −1 hollow-fibre). Cibacron Blue F3ga attachment onto the hollow-fibres significantly increased the HSA adsorption (147 mg g −1 hollow-fibre). The maximum HSA adsorption was observed at pH 5.0. Higher HSA adsorption was observed from human plasma (230 mg HSA g −1 hollow-fibre). Desorption of HSA from Cibacron Blue F3ga derived hollow-fibres was obtained using 0.1 M Tris–HCl buffer containing 0.5 M NaSCN or 1.0 M NaCl. High desorption ratios (up to 98% of the adsorbed HSA) were observed. It was possible to reuse Cibacron Blue F3ga derived polyamide hollow-fibre without significant decreases in the adsorption capacities.
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albumin adsorption from aqueous solutions and human plasma in a packed bed column with Cibacron Blue F3ga zn ii attached poly egdma hema microbeads
Reactive & Functional Polymers, 1999Co-Authors: Adil Denizli, Handan Yavuz, Gulay Kokturk, Erhan PiskinAbstract:Affinity dye-ligand Cibacron Blue F3ga, was covalently coupled with poly(EGDMA-HEMA) microbeads via nucleophilic reaction between the chloride of its triazine ring and the hydroxyl groups of the HEMA under alkaline conditions. The microbeads carrying 16.5 μmol Cibacron Blue F3ga per gram polymer was incorporated with Zn(II) ions. Zn(II) loading was 189.6 μmol/g. Cibacron Blue F3ga-Zn(II) attached affinity sorbent was used for albumin adsorption from aqueous solutions and human plasma in a packed-bed column. BSA adsorption capacity of the microbeads decreased with an increase in the recirculation rate. High adsorption rates were observed at the beginning, then equilibrium was gradually achieved in about 60 min. The BSA concentration in the mobile phase also effected adsorption. BSA adsorption was first increased with BSA concentration, then reached a plateau which was about 128 mg BSA/g. The maximum adsorption was observed at pH 5.0 which is the isoelectric pH of BSA. Higher human serum albumin adsorption was observed from human plasma (215 mg HSA/g). High desorption ratios (over 90% of the adsorbed albumin) were achieved by using 1.0 M NaSCN (pH 8.0) in 30 min.