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Krzysztof Marycz - One of the best experts on this subject based on the ideXlab platform.
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Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose derived mesenchymal stromal stem cells affected by metabolic syndrome
Stem Cell Research & Therapy, 2019Co-Authors: Krzysztof Marycz, Izabela Michalak, Lynda Bourebaba, Meriem Baouche, Katarzyna KucharczykAbstract:Chondrogenesis represents a highly dynamic cellular process that leads to the establishment of various types of cartilage. However, when stress-related injuries occur, a rapid and efficient regeneration of the tissues is necessary to maintain cartilage integrity. Mesenchymal stem cells (MSCs) are known to exhibit high capacity for self-renewal and pluripotency effects, and thus play a pivotal role in the repair and regeneration of damaged cartilage. On the other hand, the influence of certain pathological conditions such as metabolic disorders on MSCs can seriously impair their regenerative properties and thus reduce their therapeutic potential. In this investigation, we attempted to improve and potentiate the in vitro chondrogenic ability of adipose-derived mesenchymal stromal stem cells (ASCs) isolated from horses suffering from metabolic syndrome. Cultured cells in chondrogenic-inductive medium supplemented with Cladophora glomerata methanolic extract were experimented for expression of the main genes and microRNAs involved in the differentiation process using RT-PCR, for their morphological changes through confocal and scanning electron microscopy and for their physiological homeostasis. The different added concentrations of C. glomerata extract to the basic chondrogenic inductive culture medium promoted the proliferation of equine metabolic syndrome ASCs (ASCsEMS) and resulted in chondrogenic phenotype differentiation and higher mRNA expression of collagen type II, aggrecan, cartilage oligomeric matrix protein, and Sox9 among others. The results reveal an obvious inhibitory effect of hypertrophy and a strong repression of miR-145-5p, miR-146-3p, and miR-34a and miR-449a largely involved in cartilage degradation. Treated cells additionally exhibited significant reduced apoptosis and oxidative stress, as well as promoted viability and mitochondrial potentiation. Chondrogenesis in EqASCsEMS was found to be prominent after chondrogenic induction in conditions containing C. glomerata extract, suggesting that the macroalgae could be considered for the enhancement of ASC cultures and their reparative properties.
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FTIR description for the natural and enriched Cladophora glomerata.
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:FTIR description for the natural and enriched Cladophora glomerata.
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A multielemental composition of the natural and soaked biomass of Cladophora glomerata (SCA)* and the enriched soaked alga determined by ICP-OES (mg/kg d.m.).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A multielemental composition of the natural and soaked biomass of Cladophora glomerata (SCA)* and the enriched soaked alga determined by ICP-OES (mg/kg d.m.).
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A comparison of a multielemental composition of a natural Cladophora glomerata collected in August (CA) and in October 2016 (CO).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A comparison of a multielemental composition of a natural Cladophora glomerata collected in August (CA) and in October 2016 (CO).
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A multielemental composition of the natural biomass of Cladophora glomerata (CO)* before and after biosorption determined by ICP-OES (mg/kg d.m.).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A multielemental composition of the natural biomass of Cladophora glomerata (CO)* before and after biosorption determined by ICP-OES (mg/kg d.m.).
Izabela Michalak - One of the best experts on this subject based on the ideXlab platform.
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Cladophora glomerata methanolic extract promotes chondrogenic gene expression and cartilage phenotype differentiation in equine adipose derived mesenchymal stromal stem cells affected by metabolic syndrome
Stem Cell Research & Therapy, 2019Co-Authors: Krzysztof Marycz, Izabela Michalak, Lynda Bourebaba, Meriem Baouche, Katarzyna KucharczykAbstract:Chondrogenesis represents a highly dynamic cellular process that leads to the establishment of various types of cartilage. However, when stress-related injuries occur, a rapid and efficient regeneration of the tissues is necessary to maintain cartilage integrity. Mesenchymal stem cells (MSCs) are known to exhibit high capacity for self-renewal and pluripotency effects, and thus play a pivotal role in the repair and regeneration of damaged cartilage. On the other hand, the influence of certain pathological conditions such as metabolic disorders on MSCs can seriously impair their regenerative properties and thus reduce their therapeutic potential. In this investigation, we attempted to improve and potentiate the in vitro chondrogenic ability of adipose-derived mesenchymal stromal stem cells (ASCs) isolated from horses suffering from metabolic syndrome. Cultured cells in chondrogenic-inductive medium supplemented with Cladophora glomerata methanolic extract were experimented for expression of the main genes and microRNAs involved in the differentiation process using RT-PCR, for their morphological changes through confocal and scanning electron microscopy and for their physiological homeostasis. The different added concentrations of C. glomerata extract to the basic chondrogenic inductive culture medium promoted the proliferation of equine metabolic syndrome ASCs (ASCsEMS) and resulted in chondrogenic phenotype differentiation and higher mRNA expression of collagen type II, aggrecan, cartilage oligomeric matrix protein, and Sox9 among others. The results reveal an obvious inhibitory effect of hypertrophy and a strong repression of miR-145-5p, miR-146-3p, and miR-34a and miR-449a largely involved in cartilage degradation. Treated cells additionally exhibited significant reduced apoptosis and oxidative stress, as well as promoted viability and mitochondrial potentiation. Chondrogenesis in EqASCsEMS was found to be prominent after chondrogenic induction in conditions containing C. glomerata extract, suggesting that the macroalgae could be considered for the enhancement of ASC cultures and their reparative properties.
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Influence of the Static Magnetic Field and Algal Extract on the Germination of Soybean Seeds
Open Chemistry, 2019Co-Authors: Sylwia Lewandowska, Izabela Michalak, Katarzyna Niemczyk, Jerzy Detyna, Henryk Bujak, Pelin ArikAbstract:AbstractThis study examines the effect of a separate static magnetic field (SMF) and algal extract and their synergistic effect on soybean seeds germination. To our knowledge, this is the first time these kinds of factors were used for the biostimulation of soybean seeds germination. Soybean – Glycine max (L.) Merrill variety ‘Merlin’ was used in the present study. The exposure of seeds to the magnetic field was applied for 3, 6 and 12 min. The algal extract, produced from a freshwater green macroalga – Cladophora glomerata using an ultrasonic homogenizer, was used directly to the paper substrate at a dose of 10%. The highest germination ability of soybean seeds was observed in a group, where the magnetic field (12 min.) was used together with 10% of algal extract. However, it was very low – only 21%, which may have resulted from the seed dormancy. Future experiments on soybean seeds are required to confirm the stimulation effect of the magnetic field (various induction values) and algal extract on seeds germination.
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FTIR description for the natural and enriched Cladophora glomerata.
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:FTIR description for the natural and enriched Cladophora glomerata.
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A multielemental composition of the natural and soaked biomass of Cladophora glomerata (SCA)* and the enriched soaked alga determined by ICP-OES (mg/kg d.m.).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A multielemental composition of the natural and soaked biomass of Cladophora glomerata (SCA)* and the enriched soaked alga determined by ICP-OES (mg/kg d.m.).
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A comparison of a multielemental composition of a natural Cladophora glomerata collected in August (CA) and in October 2016 (CO).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A comparison of a multielemental composition of a natural Cladophora glomerata collected in August (CA) and in October 2016 (CO).
Małgorzata Mironiuk - One of the best experts on this subject based on the ideXlab platform.
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FTIR description for the natural and enriched Cladophora glomerata.
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:FTIR description for the natural and enriched Cladophora glomerata.
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A multielemental composition of the natural and soaked biomass of Cladophora glomerata (SCA)* and the enriched soaked alga determined by ICP-OES (mg/kg d.m.).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A multielemental composition of the natural and soaked biomass of Cladophora glomerata (SCA)* and the enriched soaked alga determined by ICP-OES (mg/kg d.m.).
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A comparison of a multielemental composition of a natural Cladophora glomerata collected in August (CA) and in October 2016 (CO).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A comparison of a multielemental composition of a natural Cladophora glomerata collected in August (CA) and in October 2016 (CO).
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A multielemental composition of the natural biomass of Cladophora glomerata (CO)* before and after biosorption determined by ICP-OES (mg/kg d.m.).
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:A multielemental composition of the natural biomass of Cladophora glomerata (CO)* before and after biosorption determined by ICP-OES (mg/kg d.m.).
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The weight percentage (wt %, mean±SD) of elements in the natural and soaked biomass of Cladophora glomerata* before and after biosorption determined by SEM-EDX.
2018Co-Authors: Izabela Michalak, Małgorzata Mironiuk, Krzysztof MaryczAbstract:The weight percentage (wt %, mean±SD) of elements in the natural and soaked biomass of Cladophora glomerata* before and after biosorption determined by SEM-EDX.
Ahmad Tavasoli - One of the best experts on this subject based on the ideXlab platform.
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hydrothermal gasification of Cladophora glomerata macroalgae over its hydrochar as a catalyst for hydrogen rich gas production
Bioresource Technology, 2016Co-Authors: Farid Safari, Omid Norouzi, Ahmad TavasoliAbstract:Abstract A tubular batch micro-reactor system was used for hydrothermal gasification (HTG) of Cladophora glomerata ( C. glomerata ) as green macroalgae found in the southern coast of the Caspian Sea, Iran. Non-catalytic tests were performed to determine the optimum condition for hydrogen production. Hydrochar, as a solid residue of non-catalytic HTG was characterized by BET, FESEM, and ICP-OES methods to determine its physiochemical properties. Surface area and pore volume of C. glomerata increased drastically after HTG. Also, the aqueous products were identified and quantified by GC–MS and GC-FID methods. Hydrochar was loaded to the reactor to determine its catalytic effect on HTG. HTG was promoted by inorganic compounds in the hydrochar and its porosity. The maximum hydrogen yield of 9.63 mmol/g was observed in the presence of algal hydrochar with the weight ratio of 0.4 to feedstock. Also, acids production was inhibited while phenol production was promoted in the presence of hydrochar.
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promotion of hydrogen rich gas and phenolic rich bio oil production from green macroalgae Cladophora glomerata via pyrolysis over its bio char
Bioresource Technology, 2016Co-Authors: Omid Norouzi, Farid Safari, Sajedeh Jafarian, Ahmad Tavasoli, Behnam NejatiAbstract:Abstract Conversion of Cladophora glomerata ( C. glomerata ) as a Caspian Sea’s green macroalgae into gaseous, liquid and solid products was carried out via pyrolysis at different temperatures to determine its potential for bio-oil and hydrogen-rich gas production for further industrial utilization. Non-catalytic tests were performed to determine the optimum condition for bio-oil production. The highest portion of bio-oil was retrieved at 500 °C. The catalytic test was performed using the bio-char derived at 500 °C as a catalyst. Effect of the addition of the algal bio-char on the composition of the bio-oil and also gaseous products was investigated. Pyrolysis derived bio-char was characterized by BET, FESEM and ICP method to show its surface area, porosity, and presence of inorganic metals on its surface, respectively. Phenols were increased from 8.5 to 20.76 area% by the addition of bio-char. Moreover, the hydrogen concentration and hydrogen selectivity were also enhanced by the factors of 1.37, 1.59 respectively.
Omid Norouzi - One of the best experts on this subject based on the ideXlab platform.
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Catalytic Effect of Functional and Fe Composite Biochars on Biofuel and Biochemical Derived from the Pyrolysis of Green Marine Biomass
'MDPI AG', 2018Co-Authors: Omid Norouzi, Francesco Di MariaAbstract:This study investigated the behavior of two types of modified biochar (functional and iron composite biochars) as a catalyst regarding their surface chemistry and morphological properties and their effects on bio-product derived from pyrolysis of Cladophora glomerata (C. glomerata) macroalagae. Two catalytic pyrolysis experiments were conducted in 25 mL slow pyrolysis reactor in the presence of biochar-based catalysts at the temperature of 500 °C. For functional biochar, no clear effect on biogas production was observed, whereas iron composite biochar increased the hydrogen content by 7.99 mml/g algae. Iron composite biochar with a 3D network structure demonstrated remarkable catalytic behaviors (especially toward hydrogen production) due to its wonderful surface area, high dispersion of iron particles and particular structures and compositions. The biochar derived marine biomass and treatment process developed here could provide a promising path for the low-cost, efficient, renewable and environmental friendly catalysts
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hydrothermal gasification of Cladophora glomerata macroalgae over its hydrochar as a catalyst for hydrogen rich gas production
Bioresource Technology, 2016Co-Authors: Farid Safari, Omid Norouzi, Ahmad TavasoliAbstract:Abstract A tubular batch micro-reactor system was used for hydrothermal gasification (HTG) of Cladophora glomerata ( C. glomerata ) as green macroalgae found in the southern coast of the Caspian Sea, Iran. Non-catalytic tests were performed to determine the optimum condition for hydrogen production. Hydrochar, as a solid residue of non-catalytic HTG was characterized by BET, FESEM, and ICP-OES methods to determine its physiochemical properties. Surface area and pore volume of C. glomerata increased drastically after HTG. Also, the aqueous products were identified and quantified by GC–MS and GC-FID methods. Hydrochar was loaded to the reactor to determine its catalytic effect on HTG. HTG was promoted by inorganic compounds in the hydrochar and its porosity. The maximum hydrogen yield of 9.63 mmol/g was observed in the presence of algal hydrochar with the weight ratio of 0.4 to feedstock. Also, acids production was inhibited while phenol production was promoted in the presence of hydrochar.
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promotion of hydrogen rich gas and phenolic rich bio oil production from green macroalgae Cladophora glomerata via pyrolysis over its bio char
Bioresource Technology, 2016Co-Authors: Omid Norouzi, Farid Safari, Sajedeh Jafarian, Ahmad Tavasoli, Behnam NejatiAbstract:Abstract Conversion of Cladophora glomerata ( C. glomerata ) as a Caspian Sea’s green macroalgae into gaseous, liquid and solid products was carried out via pyrolysis at different temperatures to determine its potential for bio-oil and hydrogen-rich gas production for further industrial utilization. Non-catalytic tests were performed to determine the optimum condition for bio-oil production. The highest portion of bio-oil was retrieved at 500 °C. The catalytic test was performed using the bio-char derived at 500 °C as a catalyst. Effect of the addition of the algal bio-char on the composition of the bio-oil and also gaseous products was investigated. Pyrolysis derived bio-char was characterized by BET, FESEM and ICP method to show its surface area, porosity, and presence of inorganic metals on its surface, respectively. Phenols were increased from 8.5 to 20.76 area% by the addition of bio-char. Moreover, the hydrogen concentration and hydrogen selectivity were also enhanced by the factors of 1.37, 1.59 respectively.