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Christophe Gadenne - One of the best experts on this subject based on the ideXlab platform.
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Sublethal Exposure Effects of the Neonicotinoid Clothianidin Strongly Modify the Brain Transcriptome and Proteome in the Male Moth Agrotis ipsilon
Insects, 2021Co-Authors: Camille Meslin, Christophe Gadenne, Sylvia Anton, Françoise Bozzolan, Virginie Braman, Solenne Chardonnet, Cédric Pionneau, Marie-christine François, Dany Severac, Martine MaibècheAbstract:Insect pest management relies mainly on neurotoxic insecticides, including neonicotinoids such as Clothianidin. The residual accumulation of low concentrations of these insecticides can have positive effects on target pest insects by enhancing various life traits. Because pest insects often rely on sex pheromones for reproduction and olfactory synaptic transmission is cholinergic, neonicotinoid residues could indeed modify chemical communication. We recently showed that treatments with low doses of Clothianidin could induce hormetic effects on behavioral and neuronal sex pheromone responses in the male moth, Agrotis ipsilon. In this study, we used high-throughput RNAseq and proteomic analyses from brains of A. ipsilon males that were intoxicated with a low dose of Clothianidin to investigate the molecular mechanisms leading to the observed hormetic effect. Our results showed that Clothianidin induced significant changes in transcript levels and protein quantity in the brain of treated moths: 1229 genes and 49 proteins were differentially expressed upon Clothianidin exposure. In particular, our analyses highlighted a regulation in numerous enzymes as a possible detoxification response to the insecticide and also numerous changes in neuronal processes, which could act as a form of acclimatization to the insecticide-contaminated environment, both leading to enhanced neuronal and behavioral responses to sex pheromone.
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Low doses of a neonicotinoid insecticide modify pheromone response thresholds of central but not peripheral olfactory neurons in a pest insect
Proceedings of the Royal Society B: Biological Sciences, 2016Co-Authors: Kaouther Rabhi, Nina Deisig, Elodie Demondion, Julie Le Corre, Guillaume Robert, Hélène Tricoire-leignel, Philippe Lucas, Christophe Gadenne, Sylvia AntonAbstract:Insect pest management relies mainly on neurotoxic insecticides, including neonicotinoids, leaving residues in the environment. There is now evidence that low doses of insecticides can have positive effects on pest insects by enhancing various life traits. Because pest insects often rely on sex pheromones for reproduction, and olfactory synaptic transmission is cholinergic, neonicotinoid residues could modify chemical communication. We recently showed that treatments with different sublethal doses of Clothianidin could either enhance or decrease behavioural sex pheromone responses in the male moth, Agrotis ipsilon. We investigated now effects of the behaviourally active Clothianidin doses on the sensitivity of the peripheral and central olfactory system. We show with extracellular recordings that both tested Clothianidin doses do not influence pheromone responses in olfactory receptor neurons. Similarly, in vivo optical imaging does not reveal any changes in glomerular response intensities to the sex pheromone after Clothianidin treatments. The sensitivity of intracellularly recorded antennal lobe output neurons, however, is upregulated by a lethal dose 20 times and downregulated by a dose 10 times lower than the lethal dose 0. This correlates with the changes of behavioural responses after Clothianidin treatment and suggests the antennal lobe as neural substrate involved in Clothianidin-induced behavioural changes.
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An insecticide further enhances experience-dependent increased behavioural responses to sex pheromone in a pest insect
PLoS ONE, 2016Co-Authors: Antoine Abrieux, Kaouther Rabhi, Hélène Tricoire-leignel, Sylvia Anton, Amel Mhamdi, Julie Egon, Stéphane Debernard, Line Duportets, Christophe GadenneAbstract:Neonicotinoid insecticides are widely used to protect plants against pest insects, and insecticide residues remaining in the environment affect both target and non-target organisms. Whereas low doses of neonicotinoids have been shown to disturb the behaviour of pollinating insects, recent studies have revealed that a low dose of the neonicotinoid Clothianidin can improve behavioural and neuronal sex pheromone responses in a pest insect, the male moth Agrotis ipsilon, and thus potentially improve reproduction. As male moth behaviour depends also on its physiological state and previous experience with sensory signals, we wondered if insecticide effects would be dependent on plasticity of olfactory-guided behaviour. We investigated, using wind tunnel experiments, whether a brief pre-exposure to the sex pheromone could enhance the behavioural response to this important signal in the moth A. ipsilon at different ages (sexually immature and mature males) and after different delays (2 h and 24 h), and if the insecticide Clothianidin would interfere with age effects or the potential pre-exposure-effects. Brief pre-exposure to the pheromone induced an age-independent significant increase of sex pheromone responses 24 h later, whereas sex pheromone responses did not increase significantly 2 h after exposure. However, response delays were significantly shorter compared to naive males already two hours after exposure. Oral treatment with Clothianidin increased sex pheromone responses in sexually mature males, confirming previous results, but did not influence responses in young immature males. Males treated with Clothianidin after pre-exposure at day 4 responded significantly more to the sex pheromone at day 5 than males treated with Clothianidin only and than males pre-exposed only, revealing an additive effect of experience and the insecticide. Plasticity of sensory systems has thus to be taken into account when investigating the effects of sublethal doses of insecticides on behaviour.
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Unexpected Effects of Low Doses of a Neonicotinoid Insecticide on Behavioral Responses to Sex Pheromone in a Pest Insect
PLoS ONE, 2014Co-Authors: Kaouther Rabhi, Julie Le Corre, Hélène Tricoire-leignel, Sylvia Anton, Kali Esancy, Anouk Voisin, Lucille Crespin, Christophe GadenneAbstract:In moths, which include many agricultural pest species, males are attracted by female-emitted sex pheromones. Although integrated pest management strategies are increasingly developed, most insect pest treatments rely on widespread use of neurotoxic chemicals, including neonicotinoid insecticides. Residual accumulation of low concentrations of these insecticides in the environment is known to be harmful to beneficial insects such as honey bees. This environmental stress probably acts as an “info-disruptor” by modifying the chemical communication system, and therefore decreases chances of reproduction in target insects that largely rely on olfactory communication. However, low doses of pollutants could on the contrary induce adaptive processes in the olfactory pathway, thus enhancing reproduction. Here we tested the effects of acute oral treatments with different low doses of the neonicotinoid Clothianidin on the behavioral responses to sex pheromone in the moth Agrotis ipsilon using wind tunnel experiments. We show that low doses of Clothianidin induce a biphasic effect on pheromone-guided behavior. Surprisingly, we found a hormetic-like effect, improving orientation behavior at the LD20 dose corresponding to 10 ng Clothianidin. On the contrary, a negative effect, disturbing orientation behavior, was elicited by a treatment with a dose below the LD0 dose corresponding to 0.25 ng Clothianidin. No Clothianidin effect was observed on behavioral responses to plant odor. Our results indicate that risk assessment has to include unexpected effects of residues on the life history traits of pest insects, which could then lead to their adaptation to environmental stress
Sylvia Anton - One of the best experts on this subject based on the ideXlab platform.
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Sublethal Exposure Effects of the Neonicotinoid Clothianidin Strongly Modify the Brain Transcriptome and Proteome in the Male Moth Agrotis ipsilon
Insects, 2021Co-Authors: Camille Meslin, Christophe Gadenne, Sylvia Anton, Françoise Bozzolan, Virginie Braman, Solenne Chardonnet, Cédric Pionneau, Marie-christine François, Dany Severac, Martine MaibècheAbstract:Insect pest management relies mainly on neurotoxic insecticides, including neonicotinoids such as Clothianidin. The residual accumulation of low concentrations of these insecticides can have positive effects on target pest insects by enhancing various life traits. Because pest insects often rely on sex pheromones for reproduction and olfactory synaptic transmission is cholinergic, neonicotinoid residues could indeed modify chemical communication. We recently showed that treatments with low doses of Clothianidin could induce hormetic effects on behavioral and neuronal sex pheromone responses in the male moth, Agrotis ipsilon. In this study, we used high-throughput RNAseq and proteomic analyses from brains of A. ipsilon males that were intoxicated with a low dose of Clothianidin to investigate the molecular mechanisms leading to the observed hormetic effect. Our results showed that Clothianidin induced significant changes in transcript levels and protein quantity in the brain of treated moths: 1229 genes and 49 proteins were differentially expressed upon Clothianidin exposure. In particular, our analyses highlighted a regulation in numerous enzymes as a possible detoxification response to the insecticide and also numerous changes in neuronal processes, which could act as a form of acclimatization to the insecticide-contaminated environment, both leading to enhanced neuronal and behavioral responses to sex pheromone.
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Low doses of a neonicotinoid insecticide modify pheromone response thresholds of central but not peripheral olfactory neurons in a pest insect
Proceedings of the Royal Society B: Biological Sciences, 2016Co-Authors: Kaouther Rabhi, Nina Deisig, Elodie Demondion, Julie Le Corre, Guillaume Robert, Hélène Tricoire-leignel, Philippe Lucas, Christophe Gadenne, Sylvia AntonAbstract:Insect pest management relies mainly on neurotoxic insecticides, including neonicotinoids, leaving residues in the environment. There is now evidence that low doses of insecticides can have positive effects on pest insects by enhancing various life traits. Because pest insects often rely on sex pheromones for reproduction, and olfactory synaptic transmission is cholinergic, neonicotinoid residues could modify chemical communication. We recently showed that treatments with different sublethal doses of Clothianidin could either enhance or decrease behavioural sex pheromone responses in the male moth, Agrotis ipsilon. We investigated now effects of the behaviourally active Clothianidin doses on the sensitivity of the peripheral and central olfactory system. We show with extracellular recordings that both tested Clothianidin doses do not influence pheromone responses in olfactory receptor neurons. Similarly, in vivo optical imaging does not reveal any changes in glomerular response intensities to the sex pheromone after Clothianidin treatments. The sensitivity of intracellularly recorded antennal lobe output neurons, however, is upregulated by a lethal dose 20 times and downregulated by a dose 10 times lower than the lethal dose 0. This correlates with the changes of behavioural responses after Clothianidin treatment and suggests the antennal lobe as neural substrate involved in Clothianidin-induced behavioural changes.
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An insecticide further enhances experience-dependent increased behavioural responses to sex pheromone in a pest insect
PLoS ONE, 2016Co-Authors: Antoine Abrieux, Kaouther Rabhi, Hélène Tricoire-leignel, Sylvia Anton, Amel Mhamdi, Julie Egon, Stéphane Debernard, Line Duportets, Christophe GadenneAbstract:Neonicotinoid insecticides are widely used to protect plants against pest insects, and insecticide residues remaining in the environment affect both target and non-target organisms. Whereas low doses of neonicotinoids have been shown to disturb the behaviour of pollinating insects, recent studies have revealed that a low dose of the neonicotinoid Clothianidin can improve behavioural and neuronal sex pheromone responses in a pest insect, the male moth Agrotis ipsilon, and thus potentially improve reproduction. As male moth behaviour depends also on its physiological state and previous experience with sensory signals, we wondered if insecticide effects would be dependent on plasticity of olfactory-guided behaviour. We investigated, using wind tunnel experiments, whether a brief pre-exposure to the sex pheromone could enhance the behavioural response to this important signal in the moth A. ipsilon at different ages (sexually immature and mature males) and after different delays (2 h and 24 h), and if the insecticide Clothianidin would interfere with age effects or the potential pre-exposure-effects. Brief pre-exposure to the pheromone induced an age-independent significant increase of sex pheromone responses 24 h later, whereas sex pheromone responses did not increase significantly 2 h after exposure. However, response delays were significantly shorter compared to naive males already two hours after exposure. Oral treatment with Clothianidin increased sex pheromone responses in sexually mature males, confirming previous results, but did not influence responses in young immature males. Males treated with Clothianidin after pre-exposure at day 4 responded significantly more to the sex pheromone at day 5 than males treated with Clothianidin only and than males pre-exposed only, revealing an additive effect of experience and the insecticide. Plasticity of sensory systems has thus to be taken into account when investigating the effects of sublethal doses of insecticides on behaviour.
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Unexpected Effects of Low Doses of a Neonicotinoid Insecticide on Behavioral Responses to Sex Pheromone in a Pest Insect
PLoS ONE, 2014Co-Authors: Kaouther Rabhi, Julie Le Corre, Hélène Tricoire-leignel, Sylvia Anton, Kali Esancy, Anouk Voisin, Lucille Crespin, Christophe GadenneAbstract:In moths, which include many agricultural pest species, males are attracted by female-emitted sex pheromones. Although integrated pest management strategies are increasingly developed, most insect pest treatments rely on widespread use of neurotoxic chemicals, including neonicotinoid insecticides. Residual accumulation of low concentrations of these insecticides in the environment is known to be harmful to beneficial insects such as honey bees. This environmental stress probably acts as an “info-disruptor” by modifying the chemical communication system, and therefore decreases chances of reproduction in target insects that largely rely on olfactory communication. However, low doses of pollutants could on the contrary induce adaptive processes in the olfactory pathway, thus enhancing reproduction. Here we tested the effects of acute oral treatments with different low doses of the neonicotinoid Clothianidin on the behavioral responses to sex pheromone in the moth Agrotis ipsilon using wind tunnel experiments. We show that low doses of Clothianidin induce a biphasic effect on pheromone-guided behavior. Surprisingly, we found a hormetic-like effect, improving orientation behavior at the LD20 dose corresponding to 10 ng Clothianidin. On the contrary, a negative effect, disturbing orientation behavior, was elicited by a treatment with a dose below the LD0 dose corresponding to 0.25 ng Clothianidin. No Clothianidin effect was observed on behavioral responses to plant odor. Our results indicate that risk assessment has to include unexpected effects of residues on the life history traits of pest insects, which could then lead to their adaptation to environmental stress
Minghua Wang - One of the best experts on this subject based on the ideXlab platform.
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dual labeled time resolved fluoroimmunoassay for simultaneous detection of Clothianidin and diniconazole in agricultural samples
Food Chemistry, 2016Co-Authors: Enze Sheng, Liangliang Zhou, Lu Feng, Tong Yu, Minghua WangAbstract:Abstract Europium (Eu 3+ ) and samarium (Sm 3+ ) were used as fluorescent labels to develop a highly sensitive dual-labeled time-resolved fluoroimmunoassay (TRFIA) for detect Clothianidin and diniconazole in food samples. Under the optimized assay conditions, 50% inhibition concentration (IC 50 ) and the limit of detection (LOD, IC 10 ) of Clothianidin were 5.08 and 0.021 μg/L, and 13.14 and 0.029 μg/L for diniconazole. The cross-reactivities (CRs) were negligible except dinotefuran (9.4%) and uniconazole (4.28%). The recoveries of Clothianidin and diniconazole ranged from 79.3% to 108.7% in food samples. The results of TRFIA for the authentic samples were validated by gas chromatography (GC) analyses, and a satisfactory correlations were obtained. These results indicated that the method was an alternative tool for simultaneous detection of Clothianidin and diniconazole in food samples.
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quantum dots based fluoroimmunoassay for the simultaneous detection of Clothianidin and thiacloprid in environmental and agricultural samples
RSC Advances, 2015Co-Authors: Ming Li, Minghua WangAbstract:Quantum dots (QDs), luminescent semiconductor nanocrystals, have the potential to simplify the performance of multi-analyte analysis. In this work, a novel indirect competitive fluorescence-linked immunosorbent assay (FLISA) based on QDs for the detection of Clothianidin and thiacloprid simultaneously in environmental and agricultural samples were performed in a single well of the microtiter plate. The QDs-labeled antibody conjugates, which consists of CdSe/ZnS core–shell QDs and polyclonal antibodies, were prepared through the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) coupling method. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of the FLISA were 12.5 and 0.3 ng mL−1 for Clothianidin, and 9.27 and 0.4 ng mL−1 for thiacloprid, respectively. The cross-reactivities of the FLISA with the analogues of Clothianidin and thiacloprid were negligible except for dinotefuran (10.4% for Clothianidin). The spiked recoveries were 73.3% to 113.1% with relative standard deviations (RSDs) of 2.7 to 13.3% for the detection of Clothianidin and thiacloprid in river water, soil, cabbage, rice and tomato. Furthermore, the results of FLISA for the authentic samples correlated well with those obtained by HPLC. The proposed FLISA is a satisfactory tool for rapid, sensitive, and quantitative detection of two pesticides simultaneously in agricultural and environmental samples. This study provides a strategy for the development of QDs-based fluoroimmunoassay for the quantitative analysis of two pesticides.
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detecting Clothianidin residues in environmental and agricultural samples using rapid sensitive enzyme linked immunosorbent assay and gold immunochromatographic assay
Science of The Total Environment, 2014Co-Authors: Xiude Hua, Liangliang Zhou, Jisong Liu, Minghua WangAbstract:Two rapid, sensitive immunoassays based on monoclonal antibody for detecting Clothianidin were developed and applied in agricultural samples: a quantitative enzyme-linked immunosorbent assay (ELISA) and a semiquantitative gold immunochromatographic assay (GICA). Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of Clothianidin were 25.6 and 3.8 ng mL(-1) for ELISA. GICA using colloidal gold-MAb probe had a visual detection limit of 8 ng mL(-1), and the results can be judged by the naked eye within 10 min. The cross-reactivities of the immunoassays with its analogues were negligible except for that with dinotefuran. For the spiked agricultural samples, recoveries of 78.0 to 114.5% with relative standard deviations (RSDs) of 3.2 to 12.8% were achieved for ELISA and further evaluated by GICA. Furthermore, the results of ELISA and GICA for the authentic samples correlated well with those obtained by HPLC. Overall, the proposed ELISA and GICA are satisfactory for rapid, sensitive, and quantitative/semiquantitative detection of Clothianidin residues in agricultural samples.
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fluorescence polarization immunoassay for highly efficient detection of Clothianidin in agricultural samples
Analytical Methods, 2014Co-Authors: Xiaofeng Liu, Xiude Hua, Wei Yin, Qingkui Fang, Minghua WangAbstract:A homogeneous fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody was successfully developed and applied in the determination of Clothianidin in agricultural samples. Three fluorescein-labeled Clothianidin tracers containing different bridge lengths (2-, 4- and 6-carbon bridges) were synthesized and employed to investigate the effect of tracer structures on the FPIA. Under optimal conditions, the fluoresceinthiocarbamyl hexamethylenediamine-labeled Clothianidin conjugate (HMDF-labeled Clothianidin), which contains a 6-carbon bridge, performed most sensitively in the FPIA for detecting Clothianidin, and the detection was achieved by one step in 12 min with a limit of detection of 5.53 ng mL−1 and an IC50 of 0.0873 μg mL−1. There were no obvious cross-reactivities of the antibodies with the analogues except for dinotefuran. Recoveries, measured in spiked water, soil, rice, apple, and cabbage samples, ranged from 78.7% to 108.2% with relative standard deviations less than 12.9%. Furthermore, the FPIA for authentic samples showed reliability and high correlations with ELISA of 0.9845 and HPLC of 0.9653. The developed FPIA demonstrated high sensitivity and accuracy, and could be ideally suitable for rapid, simple, and high-throughput screening for Clothianidin contamination in agricultural samples.
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development of immunoassays for detecting Clothianidin residue in agricultural products
Journal of Agricultural and Food Chemistry, 2013Co-Authors: Enze Sheng, Lujing Cong, Minghua WangAbstract:Two enzyme-linked immunosorbent assays (ELISAs) based on polyclonal antibodies (PcAbs) for Clothianidin are described: colorimetric detection format (ELISA) and pattern of chemiluminescent assay (CLEIA). Clothianidin hapten was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to produce immunogen and coating antigen. AntiClothianidin PcAbs were obtained from immunized New Zealand white rabbits. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC20) of Clothianidin were 0.046 and 0.0028 mg/L for the ELISA and 0.015 and 0.0014 mg/L for the CLEIA, respectively. There were no obvious cross-reactivities of the antibodies with its analogues except for dinotefuran. Recoveries of 76.4–116.4% for the immunoassays were achieved from spiked samples. The results of immunoassays for the spiked and authentic samples were largely consistent with gas chromatography. Therefore, the proposed immunoassays would be convenient and satisfact...
Feng Liu - One of the best experts on this subject based on the ideXlab platform.
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influence of lethal and sublethal exposure to Clothianidin on the seven spotted lady beetle coccinella septempunctata l coleoptera coccinellidae
Ecotoxicology and Environmental Safety, 2018Co-Authors: Jiangong Jiang, Zhengqun Zhang, Feng LiuAbstract:Abstract The seven-spotted ladybird beetle, Coccinella septempunctata L., as a dominant predator of aphids, has played a crucial role in integrated pest management (IPM) strategies in agricultural ecosystems. To study the risk of insecticides to C. septempunctata, the neonicotinoid Clothianidin was selected for evaluation of its influence on C. septempunctata at lethal and sublethal doses. The LR50 (application rate causing 50% mortality) in the exposed larvae decreased from 19.94 to 5.91 g a.i. ha−1, and the daily HQ (hazard quotient) values increased from 3.00 to 10.15, indicating potential intoxication risks. We also determined NOERs (No Observed Effect application Rates) of Clothianidin on the total developmental time (10 g a.i. ha−1), survival (2.5 g a.i. ha−1) and pupation (5 g a.i. ha−1). Moreover, Clothianidin at a NOER of 2.5 g a.i. ha−1 did not profoundly affect adult emergence, fecundity or egg hatchability. The total effect (E) assessment also showed that Clothianidin at 2.5 g a.i. ha−1 was slightly harmful to C. septempunctata. These results suggested that Clothianidin would impair C. septempunctata when applied at over 2.5 g a.i. ha−1 in the field. Conservation of this biological control agent in agricultural ecosystems thus requires further measures to decrease the applied dosages of Clothianidin.
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dissipation dynamics of Clothianidin and its control efficacy against bradysia odoriphaga yang and zhang in chinese chive ecosystems
Pest Management Science, 2016Co-Authors: Peng Zhang, Yunhe Zhao, Yupeng Ren, Yan Wei, Feng LiuAbstract:BACKGROUND Clothianidin is a second-generation neonicotinoid insecticide that is quite effective against Bradysia odoriphaga Yang and Zhang, the major insect pest affecting Chinese chive in northern China. In this study, the dissipation of Clothianidin in soil and its residue in leaves and pseudostems/bulbs as well as its control efficacy against B. odoriphaga and two other secondary pests were investigated in Chinese chive fields after soil application of Clothianidin by the directional spray-washing method. RESULTS The half-life of Clothianidin was 35.73-36.10 days, and it could be detected in Chinese chive plants in both treatment plots up to 240 days after a single soil application. Clothianidin applied at 3.0 and 6.0 kg AI ha(-1) could suppress B. odoriphaga population growth, achieve satisfactory levels of pest control for almost 10 months and reduce the losses of the yield in winter. Moreover, the treatments also significantly reduced Thrips alliorum and Acrolepia alliella populations up to nearly 180 days after one application. CONCLUSION Clothianidin can be considered to show long-lasting efficacy against B. odoriphaga and to be safe for use in Chinese chive at 3.0 and 6.0 kg AI ha(-1) once in the early root-rearing period to control B. odoriphaga in these cultivation ecosystems. © 2015 Society of Chemical Industry.
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effects of imidacloprid and Clothianidin seed treatments on wheat aphids and their natural enemies on winter wheat
Pest Management Science, 2016Co-Authors: Peng Zhang, Yunhe Zhao, Yan Wei, Xuefeng Zhang, Feng LiuAbstract:BACKGROUND Wheat aphid (Hemiptera: Aphididae) is one of the major pests of winter wheat and has posed a significant threat to winter wheat production in China. Although neonicotinoid insecticidal seed treatments have been suggested to be a control method, the season-long efficacy on pests and the impact on their natural enemies are still uncertain. Experiments were conducted to determine the efficacy of imidacloprid and Clothianidin on the control of aphids, the number of their natural enemies and the emergence rate and yield of wheat during 2011–2014. RESULTS Imidacloprid and Clothianidin seed treatments had no effect on the emergence rate of winter wheat and could prevent yield losses and wheat aphid infestations throughout the winter wheat growing season. Furthermore, their active ingredients were detected in winter wheat leaves up to 200 days after sowing. Imidacloprid and Clothianidin seed treatments had no adverse effects on ladybirds, hoverflies or parasitoids, and instead increased the spider–aphid ratios. CONCLUSION Wheat seeds treated with imidacloprid and Clothianidin were effective against wheat aphids throughout the winter wheat growing season and reduced the yield loss under field conditions. Imidacloprid and Clothianidin seed treatments may be an important component of the integrated management of wheat aphids on winter wheat. © 2015 Society of Chemical Industry
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efficacy of granular applications of Clothianidin and nitenpyram against aphis gossypii glover and apolygus lucorum meyer dur in cotton fields in china
Crop Protection, 2015Co-Authors: Peng Zhang, Yunhe Zhao, Yupeng Ren, Xuefeng Zhang, Feng LiuAbstract:Abstract With the widespread use of transgenic Bt-cotton in China and the reduction in the amount of broad-spectrum insecticides used in cotton production, piercing-sucking insects, including mirids, aphids, thrips and whiteflies, have become major pests and have led to significant yield losses. Although neonicotinoid insecticidal seed or granular treatments at sowing have been suggested as a control method, the season-long efficacy of these treatments against pests and their natural enemies remains unknown. This study, conducted during 2013–2014 in Shandong, demonstrates that granular treatments of both Clothianidin and nitenpyram at sowing can prevent yield losses and Aphis gossypii (Glover) and Apolygus lucorum (Meyer-Dur) infestations during the seedling to blooming stages of Bt-cotton. Clothianidin and nitenpyram granular treatments maintained A. lucorum at densities that were below the average threshold for economic relevance. Clothianidin (applied at a rate of 3.6 kg a.i./ha) provided the longest and best control within 115 days after planting and improved the yield. These granular treatments also reduced the population densities of Frankliniella intonsa (Trybom) and Bemisia tabaci (Gennadius) but decreased the population densities of Harmonia axyridis (Pallas) and Propylea japonica (Thunberg), an effect that may be due more to a lack of prey than to insecticide toxicity. Granular treatments with Clothianidin and nitenpyram produced systemic insecticidal effects on A. gossypii and A. lucorum , with the former being slightly more effective than the latter. The use of granular treatments of these insecticides at sowing may represent an important component of integrated management practices against piercing-sucking insects in Bt-cotton.
Louisa A Messenger - One of the best experts on this subject based on the ideXlab platform.
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evaluation of toxicity of Clothianidin neonicotinoid and chlorfenapyr pyrrole insecticides and cross resistance to other public health insecticides in anopheles arabiensis from ethiopia
Malaria Journal, 2019Co-Authors: Kendra Dagg, Seth R Irish, Ryan E Wiegand, Josephat Shililu, Delenasaw Yewhalaw, Louisa A MessengerAbstract:Insecticide-based interventions play an integral role in malaria vector control. However, the continued spread of insecticide resistance threatens to undermine progress made thus far and may ultimately lead to operational failure of current control measures. Clothianidin and chlorfenapyr both have unique modes of action and have expanded the number of insecticide classes available to vector control programmes. Prior to field use, it is imperative to establish their toxicity against local mosquito populations and evaluate potential cross-resistance with other chemicals used contemporarily or historically. The aim of this study was to determine the diagnostic doses of Clothianidin and chlorfenapyr and their efficacies against Anopheles arabiensis, the predominant Ethiopian malaria vector species. A range of doses of Clothianidin and chlorfenapyr were tested, using modified WHO susceptibility tests and CDC bottle bioassays, respectively, against an Ethiopian susceptible laboratory strain and a wild population of An. arabiensis collected from Oromia Region, Ethiopia. Cross-resistance to other public health insecticides: carbamates (bendiocarb and propoxur), organophosphate (malathion) and pyrethroids (deltamethrin and permethrin), was assessed in the same mosquito populations using CDC bottle bioassays. Complete mosquito mortality was observed with the laboratory strain using the recommended diagnostic doses for Clothianidin (2%/filter paper) and chlorfenapyr (100 µg/bottle). The field population was resistant to malathion (83% mortality), capable of surviving 2×, 5× and 10× the diagnostic dose of both deltamethrin and permethrin, but susceptible to bendiocarb and propoxur. The field population of An. arabiensis was significantly more susceptible to Clothianidin, reaching 100% mortality by day 2 compared to the laboratory strain (100% mortality by day 3). In contrast, the wild population was less susceptible to chlorfenapyr, with the highest mortality of 99% at 72 h using 200 µg/bottle compared to the laboratory colony, which reached complete mortality at 50 µg/bottle by 24 h. The putative diagnostic doses of Clothianidin and chlorfenapyr are appropriate for monitoring resistance in An. arabiensis from Ethiopia. The unique modes of action and an absence of cross-resistance render Clothianidin and chlorfenapyr potential candidates for inclusion in the National Malaria Control Programme vector control efforts, particularly in areas with high pre-existing or emergent resistance to other insecticide classes.