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Narayan C. Mishra - One of the best experts on this subject based on the ideXlab platform.
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fabrication and characterization of pcl gelatin chitosan ternary nanofibrous Composite Scaffold for tissue engineering applications
Journal of Materials Science, 2014Co-Authors: Sneh Gautam, Amit Kumar Dinda, Pravin D. Potdar, Chia-fu Chou, Narayan C. MishraAbstract:In the present study, we have fabricated a ternary Composite nanofibrous Scaffold from PCL/gelatin/chitosan, by electrospinning technique, using a solvent system—chloroform/methanol for polycaprolactone (PCL) and acetic acid for gelatin and chitosan, for tissue engineering applications. Field emission scanning electron microscopy (FE-SEM) was used to investigate the fiber morphology of the Scaffold and it was found that the fiber morphology was influenced by the concentrations of PCL, gelatin, and chitosan in polymer solution during electrospinning. X-ray diffraction, Fourier transform infrared, and thermogravimetric (TG) analysis results showed some interactions among the molecules of PCL, gelatin, and chitosan within the Scaffold. In-vitro cell culture studies were done by seeding L929 mouse fibroblasts on fabricated Composite Scaffold, which confirmed the cell viability, high cell proliferation rate, and cell adhesion on Composite Scaffold as indicated by MTT assay, DNA quantification, and FE-SEM analysis of cell-Scaffold construct. Thus, the ternary Composite Scaffold made from the combination of PCL (synthetic polymer), gelatin, and chitosan (natural polymer) may find potential application in tissue engineering.
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surface modification of nanofibrous polycaprolactone gelatin Composite Scaffold by collagen type i grafting for skin tissue engineering
Materials Science and Engineering: C, 2014Co-Authors: Sneh Gautam, Amit Kumar Dinda, Pravin D. Potdar, Chia-fu Chou, Narayan C. MishraAbstract:Abstract In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I Composite nanofibrous Scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin nanofibrous Scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the nanofibrous PCL/gelatin Scaffold was modified by collagen type I (0.2–1.5 wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin Composite Scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin Scaffold by collagen type I immobilization on the surface of the Scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified Composite Scaffold. FE-SEM analysis of cell-Scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of Scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified Scaffold. Above results suggest that the collagen type I-modified PCL/gelatin Scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I Composite nanofibrous Scaffold might be a potential candidate for wound healing and skin tissue engineering applications.
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surface modification of nanofibrous polycaprolactone gelatin Composite Scaffold by collagen type i grafting for skin tissue engineering
Materials Science and Engineering: C, 2014Co-Authors: Sneh Gautam, Amit Kumar Dinda, Pravin D. Potdar, Chia-fu Chou, Narayan C. MishraAbstract:Abstract In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I Composite nanofibrous Scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin nanofibrous Scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the nanofibrous PCL/gelatin Scaffold was modified by collagen type I (0.2–1.5 wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin Composite Scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin Scaffold by collagen type I immobilization on the surface of the Scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified Composite Scaffold. FE-SEM analysis of cell-Scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of Scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified Scaffold. Above results suggest that the collagen type I-modified PCL/gelatin Scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I Composite nanofibrous Scaffold might be a potential candidate for wound healing and skin tissue engineering applications.
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Surface modification of nanofibrous polycaprolactone/gelatin Composite Scaffold by collagen type I grafting for skin tissue engineering.
Materials Science and Engineering: C, 2013Co-Authors: Sneh Gautam, Amit Kumar Dinda, Pravin D. Potdar, Chia-fu Chou, Narayan C. MishraAbstract:In the present study, a tri-polymer polycaprolactone (PCL)/gelatin/collagen type I Composite nanofibrous Scaffold has been fabricated by electrospinning for skin tissue engineering and wound healing applications. Firstly, PCL/gelatin nanofibrous Scaffold was fabricated by electrospinning using a low cost solvent mixture [chloroform/methanol for PCL and acetic acid (80% v/v) for gelatin], and then the nanofibrous PCL/gelatin Scaffold was modified by collagen type I (0.2-1.5wt.%) grafting. Morphology of the collagen type I-modified PCL/gelatin Composite Scaffold that was analyzed by field emission scanning electron microscopy (FE-SEM), showed that the fiber diameter was increased and pore size was decreased by increasing the concentration of collagen type I. Fourier transform infrared (FT-IR) spectroscopy and thermogravimetric (TG) analysis indicated the surface modification of PCL/gelatin Scaffold by collagen type I immobilization on the surface of the Scaffold. MTT assay demonstrated the viability and high proliferation rate of L929 mouse fibroblast cells on the collagen type I-modified Composite Scaffold. FE-SEM analysis of cell-Scaffold construct illustrated the cell adhesion of L929 mouse fibroblasts on the surface of Scaffold. Characteristic cell morphology of L929 was also observed on the nanofiber mesh of the collagen type I-modified Scaffold. Above results suggest that the collagen type I-modified PCL/gelatin Scaffold was successful in maintaining characteristic shape of fibroblasts, besides good cell proliferation. Therefore, the fibroblast seeded PCL/gelatin/collagen type I Composite nanofibrous Scaffold might be a potential candidate for wound healing and skin tissue engineering applications.
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fabrication and characterization of natural origin chitosan gelatin alginate Composite Scaffold by foaming method without using surfactant
Journal of Applied Polymer Science, 2013Co-Authors: Chhavi Sharma, Amit Kumar Dinda, Narayan C. MishraAbstract:A natural origin tripolymer Scaffold from chitosan, gelatin, and alginate was fabricated by applying foaming method without adding any foam stabilizing surfactant. Previously, in foaming method of Scaffold fabrication, toxic surfactants were used to stabilize the foam, but in this work, the use of surfactant has been avoided strictly, which can provide better environment for cellular response and viability. In foaming method, stable foam is produced simply by agitating the polymer (alginate-gelatin) solution, and the foam is crosslinked with CaCl2, glutaraldehyde, and chitosan to produce tripolymer alginate-gelatin-chitosan Composite Scaffold. Microscopic images of the Composite Scaffold revealed the presence of interconnected pores, mostly spread over the entire surface of the Scaffold. The Scaffold has a porosity of 90% with a mean pore size of 57 μm. Swelling and degradation studies of the Scaffold showed that the Scaffold possesses excellent properties of hydrophilicity and biodegradability. In vitro cell culture studies by seeding L929 mouse fibroblast cells on Scaffold revealed excellent cell viability, proliferation rate and adhesion as indicated by MTT assay, DNA quantification, and phase contrast microscopy of cell-Scaffold construct. The natural origin Composite Scaffold fabricated by the simplest method i.e., foaming method, but without adding any surfactant, is cheap, biocompatible, and it might find potential applications in the field of tissue engineering. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Binghong Luo - One of the best experts on this subject based on the ideXlab platform.
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Fabrication and evaluation of a chitin whisker/poly(L-lactide) Composite Scaffold by the direct trisolvent-ink writing method for bone tissue engineering.
Nanoscale, 2020Co-Authors: Kun Liu, Ling Zhu, Shengyue Tang, Wei Wen, Mingxian Liu, Changren Zhou, Binghong LuoAbstract:Although poly(L-lactide) (PLLA) based porous Scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair. Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix. Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) Composite inks. Then, the CP porous Composite Scaffolds were further fabricated via the direct ink writing method. The as-printed CP Composite Scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%. Compared with the pure PLLA Scaffold, the CP Composite Scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs. Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0–20 wt%. However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP Composite Scaffold decreased, which may be attributed to the different microstructure of the Scaffold from other Composite Scaffolds. Interestingly, compared with these Scaffolds containing a lower mass content of CHWs, only the 40%CP Composite Scaffold exhibited significant anti-inflammatory properties. These robust CP Composite Scaffolds offer a new route for bone tissue engineering application.
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fabrication and evaluation of a chitin whisker poly l lactide Composite Scaffold by the direct trisolvent ink writing method for bone tissue engineering
Nanoscale, 2020Co-Authors: Kun Liu, Ling Zhu, Shengyue Tang, Wei Wen, Mingxian Liu, Changren Zhou, Binghong LuoAbstract:Although poly(L-lactide) (PLLA) based porous Scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair. Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix. Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) Composite inks. Then, the CP porous Composite Scaffolds were further fabricated via the direct ink writing method. The as-printed CP Composite Scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%. Compared with the pure PLLA Scaffold, the CP Composite Scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs. Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0–20 wt%. However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP Composite Scaffold decreased, which may be attributed to the different microstructure of the Scaffold from other Composite Scaffolds. Interestingly, compared with these Scaffolds containing a lower mass content of CHWs, only the 40%CP Composite Scaffold exhibited significant anti-inflammatory properties. These robust CP Composite Scaffolds offer a new route for bone tissue engineering application.
Xinluan Wang - One of the best experts on this subject based on the ideXlab platform.
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plga β tcp Composite Scaffold incorporating salvianolic acid b promotes bone fusion by angiogenesis and osteogenesis in a rat spinal fusion model
Biomaterials, 2019Co-Authors: Sien Lin, Yuxiao Lai, Xinluan Wang, Liao Cui, Guanghua Chen, Jianping Huang, Yanhua Yang, Kaijie Zou, Liyi Zou, Jack C Y ChengAbstract:Abstract Spinal disorders often require surgical treatment called spinal fusion to restore a stabilized spine where bone grafts are implanted for the fusion of adjacent vertebras. In this study, we developed a bioactive Composite Scaffold incorporated with salvianolic acid B (SB), an active component extracted from Danshen. This study aimed to evaluate the effects of SB-incorporated porous Scaffold on spinal fusion models. The Composite Scaffolds composed of poly (lactic-co-glycolic acid) and tricalcium phosphate (PLGA/β-TCP) were fabricated with low-temperature rapid prototyping technique, which incorporated SB at low (SB-L), middle (SB-M), high (SB-H) doses, and pure PLGA/β-TCP as blank control (Con). The release profile of SB from the Scaffolds was determined by high performance liquid chromatography. Osteoconductive and osteoinductive properties of the Scaffolds were reflected by the osteogenic differentiation ability of rat primary mesenchymal stem cells. The angiogenesis was determined by the forming of tube-like structures resembling capillaries using endothelial cell line (EA hy9.26). A well-established spinal fusion model was used to evaluate the in vivo bony fusion. Animals were transplanted with Scaffolds, or autografts from iliac crest as positive controls. Micro-computed tomography (CT) analysis, CT-based angiography, manual palpation test, histomorphometry, and histology were performed after 8 weeks of transplantation. Results revealed that incorporated SB was steadily released from the Scaffolds. The aliquot of released SB promoted osteogenesis and angiogenesis in vitro in a dose-dependent manner. In animal study, a dose-dependent effect of SB on new bone formation, mineral apposition rate, and vessel density within the Scaffold were demonstrated. Manual palpation test showed little numerical improvement in fusion rate when compared with the blank controls. In summary, our results suggested that SB-incorporated PLGA/β-TCP Composite Scaffold could enhance bony fusion through the promotion of osteogenesis and angiogenesis.
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porous Composite Scaffold incorporating osteogenic phytomolecule icariin for promoting skeletal regeneration in challenging osteonecrotic bone in rabbits
Biomaterials, 2018Co-Authors: Yuxiao Lai, Huijuan Cao, Xinluan Wang, Shukui Chen, Ming Zhang, Nan Wang, Zhihong Yao, Yi Dai, Xinhui XieAbstract:Steroid-associated osteonecrosis (SAON) often requires surgical core decompression (CD) in the early stage for removal of necrotic bone to facilitate repair where bone grafts are needed for filling bone defect and avoiding subsequent joint collapse. In this study, we developed a bioactive Composite Scaffold incorporated with icariin, a unique phytomolecule that can provide structural and mechanical support and facilitate bone regeneration to fill into bone defects after surgical CD in established SAON rabbit model. An innovative low-temperature 3D printing technology was used to fabricate the poly (lactic-co-glycolic acid)/β-calcium phosphate/icariin (PLGA/TCP/Icariin, PTI) Scaffold. The cytocompatibility of the PTI Scaffold was tested in vitro, and the osteogenesis properties of PTI Scaffolds were assessed in vivo in the SAON rabbit models. Our results showed that the fabricated PTI Scaffold had a well-designed biomimic structure that was precisely printed to provide increased mechanical support and stable icariin release from the Scaffold for bone regeneration. Furthermore, our in vivo study indicated that the PTI Scaffold could enhanced the mechanical properties of new bone tissues and improved angiogenesis within the implanted region in SAON rabbit model than those of PLGA/TCP (PT) Scaffold. The underlying osteoblastic mechanism was investigated using MC3T3-E1 cells in vitro and revealed that icariin could facilitate MC3T3-E1 cells ingrowth into the PTI Scaffold and regulate osteoblastic differentiation. The PTI Scaffold exhibited superior biodegradability, biocompatibility, and osteogenic capability compared with those of PT Scaffold. In summary, the PTI Composite Scaffold which incorporated bioactive phyto-compounds is a promising potential strategy for bone tissue engineering and regeneration in patients with challenging SAON.
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plga tcp Composite Scaffold incorporating bioactive phytomolecule icaritin for enhancement of bone defect repair in rabbits
Acta Biomaterialia, 2013Co-Authors: S H Chen, Xinluan Wang, Xinhui Xie, Ming Lei, Lizhen Zheng, Dong Yao, Zhihe Zhao, A KongAbstract:Bone defect repair is challenging in orthopaedic clinics. For treatment of large bone defects, bone grafting remains the method of choice for the majority of surgeons, as it fills spaces and provides support to enhance biological bone repair. As therapeutic agents are desirable for enhancing bone healing, this study was designed to develop such a bioactive Composite Scaffold (PLGA/TCP/ICT) made of polylactide-co-glycolide (PLGA) and tricalcium phosphate (TCP) as a basic carrier, incorporating a phytomolecule icaritin (ICT), i.e., a novel osteogenic exogenous growth factor. PLGA/TCP/ICT Scaffolds were fabricated as PLGA/TCP (control group) and PLGA/TCP in tandem with low/mid/high-dose ICT (LICT/MICT/HICT groups, respectively). To evaluate the in vivo osteogenic and angiogenic potentials of these bioactive Scaffolds with slow release of osteogenic ICT, the authors established a 12 mm ulnar bone defect model in rabbits. X-ray and high-resolution peripheral quantitative computed tomography results at weeks 2, 4 and 8 post-surgery showed more newly formed bone within bone defects implanted with PLGA/TCP/ICT Scaffolds, especially PLGA/TCP/MICT Scaffold. Histological results at weeks 4 and 8 also demonstrated more newly mineralized bone in PLGA/TCP/ICT groups, especially in the PLGA/TCP/MICT group, with correspondingly more new vessel ingrowth. These findings may form a good foundation for potential clinical validation of this innovative bioactive Scaffold incorporated with the proper amount of osteopromotive phytomolecule ICT as a ready product for clinical applications.
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comparative study of osteogenic potential of a Composite Scaffold incorporating either endogenous bone morphogenetic protein 2 or exogenous phytomolecule icaritin an in vitro efficacy study
Acta Biomaterialia, 2012Co-Authors: S H Chen, Xinluan Wang, Xinhui Xie, Lizhen Zheng, Dong Yao, D P Wang, Yang Leng, Ge Zhang, Ling QinAbstract:A local delivery system with sustained and efficient release of therapeutic agents from an appropriate carrier is desirable for orthopedic applications. Novel Composite Scaffolds made of poly (lactic-co-glycolic acid) with tricalcium phosphate (PLGA/TCP) were fabricated by an advanced low-temperature rapid prototyping technique, which incorporated either endogenous bone morphogenetic protein-2 (BMP-2) (PLGA/TCP/BMP-2) or phytomolecule icaritin (ICT) (PLGA/TCP/ICT) at low, middle and high doses. PLGA/TCP served as control. In vitro degradation, osteogenesis and release tests showed statistical differences among PLGA/TCP/ICT, PLGA/TCP and PLGA/TCP/BMP-2 groups, where PLGA/TCP/ICT had the desired slow release of bioactive icaritin in a dose-dependent manner, whereas there was almost no BMP-2 release from the PLGA/TCP/BMP-2 Scaffolds. PLGA/TCP/ICT significantly increased more ALP activity, upregulated mRNA expression of osteogenic genes and enhanced calcium deposition and mineralization in rabbit bone marrow stem cells cultured on Scaffolds compared with the other two groups. These results indicate the desired degradation rate, osteogenic capability and release property in PLGA/TCP/ICT Composite Scaffold, as icaritin preserved its bioactivity and structure after incorporation, while PLGA/TCP/BMP-2 did not show an initially expected osteogenic potential, owing to loss of the original bioactivity of BMP-2 during its incorporation and fabrication procedure. The results suggest that PLGA/TCP Composite Scaffolds incorporating osteogenic ICT might be a promising approach for bone tissue bioengineering and regeneration.
Xifu Zheng - One of the best experts on this subject based on the ideXlab platform.
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Fabrication and cell affinity of biomimetic structured PLGA/articular cartilage ECM Composite Scaffold
Journal of materials science. Materials in medicine, 2011Co-Authors: Xifu Zheng, Aiyuan Wang, Fei Yang, Shenguo Wang, Weiguo Zhang, Shuyun Liu, Jingxiang Huang, Baosheng YinAbstract:An ideal Scaffold for cartilage tissue engineering should be biomimetic in not only mechanical property and biochemical composition, but also the morphological structure. In this research, we fabricated a Composite Scaffold with oriented structure to mimic cartilage physiological morphology, where natural nanofibrous articular cartilage extracellular matrix (ACECM) was used to mimic the biochemical composition, and synthetic PLGA was used to enhance the mechanical strength of ACECM. The Composite Scaffold has well oriented structure and more than 89% of porosity as well as about 107 μm of average pore diameter. The Composite Scaffold was compared with ACECM and PLGA Scaffolds. Cell proliferation test showed that the number of MSCs in ACECM and Composite Scaffolds was noticeably bigger than that in PLGA Scaffold, which was coincident with results of SEM observation and cell viability staining. The water absorption of ACECM and Composite Scaffolds were 22.1 and 10.2 times respectively, which was much higher than that of PLGA Scaffolds (3.8 times). The compressive modulus of Composite Scaffold in hydrous status was 1.03 MPa, which was near 10 times higher than that of hydrous ACECM Scaffold. The aforementioned results suggested that the Composite Scaffold has the potential for application in cartilage tissue engineering.
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fabrication and cell affinity of biomimetic structured plga articular cartilage ecm Composite Scaffold
Journal of Materials Science: Materials in Medicine, 2011Co-Authors: Aiyuan Wang, Fei Yang, Shenguo Wang, Xifu Zheng, Weiguo Zhang, Shuyun Liu, Jingxiang Huang, Baosheng Yin, Li ZhangAbstract:An ideal Scaffold for cartilage tissue engineering should be biomimetic in not only mechanical property and biochemical composition, but also the morphological structure. In this research, we fabricated a Composite Scaffold with oriented structure to mimic cartilage physiological morphology, where natural nanofibrous articular cartilage extracellular matrix (ACECM) was used to mimic the biochemical composition, and synthetic PLGA was used to enhance the mechanical strength of ACECM. The Composite Scaffold has well oriented structure and more than 89% of porosity as well as about 107 μm of average pore diameter. The Composite Scaffold was compared with ACECM and PLGA Scaffolds. Cell proliferation test showed that the number of MSCs in ACECM and Composite Scaffolds was noticeably bigger than that in PLGA Scaffold, which was coincident with results of SEM observation and cell viability staining. The water absorption of ACECM and Composite Scaffolds were 22.1 and 10.2 times respectively, which was much higher than that of PLGA Scaffolds (3.8 times). The compressive modulus of Composite Scaffold in hydrous status was 1.03 MPa, which was near 10 times higher than that of hydrous ACECM Scaffold. The aforementioned results suggested that the Composite Scaffold has the potential for application in cartilage tissue engineering.
Jianxun Ding - One of the best experts on this subject based on the ideXlab platform.
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high pressure compression molded porous resorbable polymer hydroxyapatite Composite Scaffold for cranial bone regeneration
ACS Biomaterials Science & Engineering, 2016Co-Authors: Jin Zhang, Jianxun Ding, Jie Wu, Xiuli Zhuang, Xuesi Chen, Jincheng Wang, Zhongming LiAbstract:Fabricating porous Scaffolds with sufficient mechanical properties is a challenge for healing bone defects. High-pressure compression-molded (HPCM) porous Composite Scaffold comprising poly(l-lactide) (PLLA), poly(lactide-co-glycolide) (PLGA), and hydroxyapatite (HA) was prepared and showed upregulated mechanical properties due to a solid network structure and a highly ordered crystalline architecture. The compressive yield strength and modulus of the HPCM Scaffold molded at 1000 MPa and 180 °C were 0.91 and 6.84 MPa, respectively. The HPCM Scaffold also exhibited an interconnected porous architecture with porosity greater than 80%, an appropriate degradation rate, and enhanced cell proliferation. Moreover, the HPCM Scaffold supported the healing of a rat calvarial defect in vivo.
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thermogel coated poly e caprolactone Composite Scaffold for enhanced cartilage tissue engineering
Polymers, 2016Co-Authors: Shaojie Wang, Zhengzheng Zhang, Dong Jiang, Yansong Qi, Haijun Wang, Jiying Zhang, Jianxun Ding, Jiakuo YuAbstract:A three-dimensional (3D) Composite Scaffold was prepared for enhanced cartilage tissue engineering, which was composed of a poly(e-caprolactone) (PCL) backbone network and a poly(lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(lactide-co-glycolide) (PLGA–PEG–PLGA) thermogel surface. The Composite Scaffold not only possessed adequate mechanical strength similar to native osteochondral tissue as a benefit of the PCL backbone, but also maintained cell-friendly microenvironment of the hydrogel. The PCL network with homogeneously-controlled pore size and total pore interconnectivity was fabricated by fused deposition modeling (FDM), and was impregnated into the PLGA–PEG–PLGA solution at low temperature (e.g., 4 °C). The PCL/Gel Composite Scaffold was obtained after gelation induced by incubation at body temperature (i.e., 37 °C). The Composite Scaffold showed a greater number of cell retention and proliferation in comparison to the PCL platform. In addition, the Composite Scaffold promoted the encapsulated mesenchymal stromal cells (MSCs) to differentiate chondrogenically with a greater amount of cartilage-specific matrix production compared to the PCL Scaffold or thermogel. Therefore, the 3D PCL/Gel Composite Scaffold may exhibit great potential for in vivo cartilage regeneration.
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Thermogel-Coated Poly(ε-Caprolactone) Composite Scaffold for Enhanced Cartilage Tissue Engineering
Polymers, 2016Co-Authors: Shaojie Wang, Zhengzheng Zhang, Dong Jiang, Haijun Wang, Jiying Zhang, Jianxun DingAbstract:A three-dimensional (3D) Composite Scaffold was prepared for enhanced cartilage tissue engineering, which was composed of a poly(e-caprolactone) (PCL) backbone network and a poly(lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(lactide-co-glycolide) (PLGA–PEG–PLGA) thermogel surface. The Composite Scaffold not only possessed adequate mechanical strength similar to native osteochondral tissue as a benefit of the PCL backbone, but also maintained cell-friendly microenvironment of the hydrogel. The PCL network with homogeneously-controlled pore size and total pore interconnectivity was fabricated by fused deposition modeling (FDM), and was impregnated into the PLGA–PEG–PLGA solution at low temperature (e.g., 4 °C). The PCL/Gel Composite Scaffold was obtained after gelation induced by incubation at body temperature (i.e., 37 °C). The Composite Scaffold showed a greater number of cell retention and proliferation in comparison to the PCL platform. In addition, the Composite Scaffold promoted the encapsulated mesenchymal stromal cells (MSCs) to differentiate chondrogenically with a greater amount of cartilage-specific matrix production compared to the PCL Scaffold or thermogel. Therefore, the 3D PCL/Gel Composite Scaffold may exhibit great potential for in vivo cartilage regeneration.