The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform
Diethard Tautz - One of the best experts on this subject based on the ideXlab platform.
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a genetic map of Cottus gobio pisces teleostei based on microsatellites can be linked to the physical map of tetraodon nigroviridis
Journal of Evolutionary Biology, 2005Co-Authors: Kathryn Stemshorn, Arne W. Nolte, Diethard TautzAbstract:To initiate QTL studies in the nonmodel fish Cottus gobio we constructed a genetic map based on 171 microsatellite markers. The mapping panel consisted of F1 intercrosses between two divergent Cottus lineages from the River Rhine System. Basic local alignment search tool (BLAST) searches with the flanking sequences of the microsatellite markers yielded a significant (e < 10−5) hit with the Tetraodon nigroviridis genomic sequence for 45% of the Cottus loci. Remarkably, most of these hits were due to short highly conserved noncoding stretches. These have an average length of 40 bp and are on average 92% conserved. Comparison of the map locations between the two genomes revealed extensive conserved synteny, suggesting that the Tetraodon genomic sequence will serve as an excellent genomic reference for at least the Acanthopterygii, which include evolutionarily interesting fish groups such as guppies (Poecilia), cichlids (Tilapia) or Xiphophorus (Platy). The apparent high density of short conserved noncoding stretches in these fish genomes will highly facilitate the identification of genes that have been identified in QTL mapping strategies of evolutionary relevant traits.
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A genetic map of Cottus gobio (Pisces, Teleostei) based on microsatellites can be linked to the physical map of Tetraodon nigroviridis
Journal of evolutionary biology, 2005Co-Authors: Kathryn Stemshorn, Arne W. Nolte, Diethard TautzAbstract:To initiate QTL studies in the nonmodel fish Cottus gobio we constructed a genetic map based on 171 microsatellite markers. The mapping panel consisted of F1 intercrosses between two divergent Cottus lineages from the River Rhine System. Basic local alignment search tool (BLAST) searches with the flanking sequences of the microsatellite markers yielded a significant (e
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Direct cloning of microsatellite loci from Cottus gobio through a simplified enrichment procedure
Molecular Ecology Notes, 2005Co-Authors: Arne W. Nolte, Kathryn Stemshorn, Diethard TautzAbstract:Applying a simplified enrichment procedure, we have isolated and characterized 177 microsatellite markers for Cottus gobio L., 1758 (Cottidae, Scorpaeniformes, Teleostei). In contrast to using specific probes for the enrichment, we use genomic DNAs of unrelated organisms for cross-hybridization. This takes advantage of the fact that simple sequences are the only repetitive elements that are abundantly found in all eukaryotic genomes and that any genome usually contains all permutations of microsatellite motifs. This cross-hybridization principle was employed to enrich genomic libraries of Cottus DNA to obtain a large number of nonredundant microsatellite markers without further screening procedures.
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phylogeography of the bullhead Cottus gobio pisces teleostei cottidae suggests a pre pleistocene origin of the major central european populations
Molecular Ecology, 2000Co-Authors: Claudia C. Englbrecht, Arne W. Nolte, Jörg Freyhof, K. Rassmann, Ulrich K. Schliewen, Diethard TautzAbstract:The bullhead Cottus gobio is a small, bottom-dwelling fish consisting of populations that have not been subject to transplantations or artificial stocking. It is therefore an ideal model species for studying the colonization history of central European freshwater systems, in particular with respect to the possible influences of the Pleistocene glaciation cycles. We sampled Cottus populations across most of its distribution range, with a special emphasis on southern Germany where the major European drainage systems are in closest contact. Mitochondrial D-loop sequencing of more than 400 specimens and phylogenetic network analysis allowed us to draw a detailed picture of the colonization of Europe by C. gobio. Moreover, the molecular distances between the haplotypes enabled us to infer an approximate time frame for the origin of the various populations. The founder population of C. gobio stems apparently from the Paratethys and invaded Europe in the Pliocene. From there, the first colonization into central Europe occurred via the ancient lower Danube, with a separate colonization of the eastern European territories. During the late Pliocene, one of the central European populations must have reached the North Sea in a second step after which it then started to colonize the Atlantic drainages via coastal lines. Accordingly, we found very distinct populations in the upper and lower Rhine, which can be explained by the fact that the lower Rhine was disconnected from the upper Rhine until approximately 1 million years ago (Ma). More closely related, but still distinct, populations were found in the Elbe, the Main and the upper Danube, all presumably of Pleistocene origin. Intriguingly, they have largely maintained their population identity, despite the strong disturbance caused by the glaciation cycles in these areas. On the other hand, a mixing of populations during postglacial recolonization could be detected in the lower Rhine and its tributaries. However, the general pattern that emerges from our analysis suggests that the glaciation cycles did not have a major impact on the general population structure of C. gobio in central Europe.
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Phylogeography of the bullhead Cottus gobio (Pisces: Teleostei: Cottidae) suggests a pre‐Pleistocene origin of the major central European populations
Molecular ecology, 2000Co-Authors: Claudia C. Englbrecht, Arne W. Nolte, Jörg Freyhof, K. Rassmann, Ulrich K. Schliewen, Diethard TautzAbstract:The bullhead Cottus gobio is a small, bottom-dwelling fish consisting of populations that have not been subject to transplantations or artificial stocking. It is therefore an ideal model species for studying the colonization history of central European freshwater systems, in particular with respect to the possible influences of the Pleistocene glaciation cycles. We sampled Cottus populations across most of its distribution range, with a special emphasis on southern Germany where the major European drainage systems are in closest contact. Mitochondrial D-loop sequencing of more than 400 specimens and phylogenetic network analysis allowed us to draw a detailed picture of the colonization of Europe by C. gobio. Moreover, the molecular distances between the haplotypes enabled us to infer an approximate time frame for the origin of the various populations. The founder population of C. gobio stems apparently from the Paratethys and invaded Europe in the Pliocene. From there, the first colonization into central Europe occurred via the ancient lower Danube, with a separate colonization of the eastern European territories. During the late Pliocene, one of the central European populations must have reached the North Sea in a second step after which it then started to colonize the Atlantic drainages via coastal lines. Accordingly, we found very distinct populations in the upper and lower Rhine, which can be explained by the fact that the lower Rhine was disconnected from the upper Rhine until approximately 1 million years ago (Ma). More closely related, but still distinct, populations were found in the Elbe, the Main and the upper Danube, all presumably of Pleistocene origin. Intriguingly, they have largely maintained their population identity, despite the strong disturbance caused by the glaciation cycles in these areas. On the other hand, a mixing of populations during postglacial recolonization could be detected in the lower Rhine and its tributaries. However, the general pattern that emerges from our analysis suggests that the glaciation cycles did not have a major impact on the general population structure of C. gobio in central Europe.
Frédéric Silvestre - One of the best experts on this subject based on the ideXlab platform.
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Effects of cadmium exposure on the gill proteome of Cottus gobio: modulatory effects of prior thermal acclimation.
Aquatic toxicology (Amsterdam Netherlands), 2014Co-Authors: Jennifer Dorts, Patrick Kestemont, Martine Raes, Marie-laetitia Thézenas, Frédéric SilvestreAbstract:Abstract Temperature and trace metals are common environmental stressors, and their importance is increasing due to global climate change and anthropogenic pollution. The aim of the present study was to investigate whether acclimation to elevated temperature affects the response of the European bullhead ( Cottus gobio ) to subsequent cadmium (Cd) exposure by using enzymatic and proteomic approaches. Fish acclimated to 15 (standard temperature), 18 or 21 °C for 28 days were exposed to 1 mg Cd/L for 4 days at the respective acclimation temperature. First, exposure to Cd significantly decreased the activity of the lactate dehydrogenase (LDH) in gills of fish acclimated to 15 or 18 °C. However, an acclimation to 21 °C suppressed the inhibitory effect of Cd. Second, using a proteomic analysis by 2D-DIGE, we observed that thermal acclimation was the first parameter affecting the protein expression profile in gills of C. gobio , while subsequent Cd exposure seemed to attenuate this temperature effect. Moreover, our results showed opposite effects of these two environmental stressors at protein expression level. From the 52 protein spots displaying significant interaction effects of temperature and Cd exposure, a total of 28 different proteins were identified using nano LC–MS/MS and the Peptide and Protein Prophet algorithms of Scaffold software. The identified differentially expressed proteins can be categorized into diverse functional classes, related to protein turnover, folding and chaperoning, metabolic process, ion transport, cell signaling and cytoskeleton. Within a same functional class, we further reported that several proteins displayed reverse responses following sequential exposure to heat and Cd. This work provides insights into the molecular pathways potentially involved in heat acclimation process and the interactive effects of temperature and Cd stress in ectothermic vertebrates.
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Proteasome and antioxidant responses in Cottus gobio during a combined exposure to heat stress and cadmium
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2011Co-Authors: Jennifer Dorts, Patrick Kestemont, Aurélie Bauwin, Sabrina Jolly, Wilfried Sanchez, Frédéric SilvestreAbstract:Abstract Temperature and trace metals are common environmental stressors, and their importance is increasing due to global climate change and anthropogenic pollution. Oxidative damage and antioxidant properties have been studied in liver and gills of the European bullhead ( Cottus gobio ) subjected to cadmium (CdCl 2 at nominal concentrations of 0.01 and 1 mg/L) for 4 days at either 15 °C or 21 °C. First, exposure to 1 mg Cd/L induced a high mortality rate (67%) in fish held at 21 °C. Regarding the antioxidant enzymes, exposure to 0.01 mg Cd/L significantly increased the activity of superoxide dismutase (SOD) and decreased the activity of glutathione reductase (GR) in liver, independently of heat stress. In gills, exposure to 21 °C resulted in a significantly increased activity of glutathione peroxidase (GPx), whereas the activity of glutathione S-transferase (GST) was significantly reduced as compared to fish exposed to 15 °C. Furthermore, regardless of Cd stress, exposure to elevated temperature resulted in a significant decrease of lipid peroxidation (LPO) level in liver and in a significant increase in the activity of chymotrypsin-like 20S proteasome in both studied tissues of C. gobio . Overall, the present results indicated that elevated temperature and cadmium exposure independently influenced the antioxidant defense system in bullhead with clear tissue-specific and stress-specific antioxidant responses. Further, elevated temperature affected the hepatic lipid peroxidation and the activity of 20S proteasome in both tissues.
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Evidence that elevated water temperature affects the reproductive physiology of the European bullhead Cottus gobio.
Fish physiology and biochemistry, 2011Co-Authors: Jennifer Dorts, Frédéric Silvestre, Gaël Grenouillet, Jessica Douxfils, Syaghalirwa N.m. Mandiki, Sylvain Milla, Patrick KestemontAbstract:Climate change is predicted to increase the average water temperature and alter the ecology and physiology of several organisms including fish species. To examine the effects of increased water temperature on freshwater fish reproduction, adult European bullhead Cottus gobio of both genders were maintained under three temperature regimes (T1: 6–10, T2: 10–14 and T3: 14–18°C) and assessed for gonad development (gonadosomatic index—GSI and gonad histology), sex steroids (testosterone—T, 17β-estradiol—E2 and 11-ketotestosterone—11-KT) and vitellogenin (alkali-labile phosphoprotein phosphorus—ALP) dynamics in December, January, February and March. The results indicate that a 8°C rise in water temperature (T3) deeply disrupted the gonadal maturation in both genders. This observation was associated with the absence of GSI peak from January to March, and low levels of plasma sex steroids compared with T1-exposed fish. Nevertheless, exposure to an increasing temperature of 4°C (T2) appeared to accelerate oogenesis with an early peak value in GSI and level of plasma T recorded in January relative to T1-exposed females. In males, the low GSI, reduced level of plasma 11-KT and the absence of GSI increase from January to March support the deleterious effects of increasing water temperature on spermatogenesis. The findings of the present study suggest that exposure to elevated temperatures within the context of climate warming might affect the reproductive success of C. gobio. Specifically, a 4°C rise in water temperature affects gametogenesis by advancing the spawning, and a complete reproductive failure is observed at an elevated temperature of 8°C.
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Ecotoxicoproteomics in gills of the sentinel fish species, Cottus gobio, exposed to perfluorooctane sulfonate (PFOS).
Aquatic toxicology (Amsterdam Netherlands), 2011Co-Authors: Jennifer Dorts, Patrick Kestemont, Martine Raes, Pierre-andré Marchand, Wendy D'hollander, Marie-laetitia Thézenas, Frédéric SilvestreAbstract:The environmental persistence, bioaccumulative tendency and potential toxicity of perfluorooctane sulfonate (PFOS) have generated great concern. This study aimed at evaluating the toxicity of short-term PFOS exposure in gills of the European bullhead Cottus gobio, a candidate sentinel species, by monitoring the response of some enzymes (citrate synthase CS, cytochrome c oxidase CCO, and lactate dehydrogenase LDH), and by undertaking a proteomic analysis using 2D-DIGE. First, a 96-h exposure to 1mg PFOS/L significantly altered the activity of mitochondrial CS and CCO. Second, 2D-DIGE gels were used to compare gills from the control fish group with tissues from fish exposed for 96h to either 0.1 or 1mg PFOS/L. From the 27 protein spots displaying significant changes in abundance following PFOS exposure, a total of 20 different proteins were identified using nano LC-MS/MS and the Peptide and Protein Prophet of Scaffold software. The differentially expressed proteins that were identified are involved in the general stress response, ubiquitin-proteasome system, energy metabolism, and actin cytoskeleton, which provide clues on the cellular pathways and components mainly affected by PFOS. Moreover, our results showed that most proteins were differentially expressed at the low but not at the high PFOS concentration. This work provides insights into the biochemical and molecular events in PFOS-induced toxicity in gill tissue, and suggests that further studies on the identified proteins could provide crucial information to better understand the mechanisms of PFOS toxicity in fish.
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Proteomic response to sublethal cadmium exposure in a sentinel fish species, Cottus gobio.
Journal of proteome research, 2010Co-Authors: Jennifer Dorts, Patrick Kestemont, Marc Dieu, Martine Raes, Frédéric SilvestreAbstract:The present study aimed at evaluating the toxicity of short-term cadmium (Cd) exposure in the European bullhead Cottus gobio, a candidate sentinel species. Several enzymatic activity assays (citrate synthase, cytochrome c oxidase, and lactate dehydrogenase) were carried out in liver and gills of fish exposed to 0.01, 0.05, 0.25, and 1 mg Cd/L for 4 days. Exposure to high Cd concentrations significantly altered the activity of these enzymes either in liver and/or in gills. Second, 2D-DIGE technique was used to identify proteins differentially expressed in tissues of fish exposed to either 0.01 or 1 mg Cd/L. Fifty-four hepatic protein spots and 37 branchial protein spots displayed significant changes in abundance in response to Cd exposure. A total of 26 and 12 different proteins were identified using nano LC-MS/MS in liver and gills, respectively. The identified differentially expressed proteins can be categorized into diverse functional classes, related to metabolic process, general stress response, protein fate, and cell structure for instance. This work provides new insights into the biochemical and molecular events in Cd-induced toxicity in fish and suggests that further studies on the identified proteins could provide crucial information to better understand the mechanisms of Cd toxicity in fish.
Jennifer Dorts - One of the best experts on this subject based on the ideXlab platform.
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Effects of cadmium exposure on the gill proteome of Cottus gobio: modulatory effects of prior thermal acclimation.
Aquatic toxicology (Amsterdam Netherlands), 2014Co-Authors: Jennifer Dorts, Patrick Kestemont, Martine Raes, Marie-laetitia Thézenas, Frédéric SilvestreAbstract:Abstract Temperature and trace metals are common environmental stressors, and their importance is increasing due to global climate change and anthropogenic pollution. The aim of the present study was to investigate whether acclimation to elevated temperature affects the response of the European bullhead ( Cottus gobio ) to subsequent cadmium (Cd) exposure by using enzymatic and proteomic approaches. Fish acclimated to 15 (standard temperature), 18 or 21 °C for 28 days were exposed to 1 mg Cd/L for 4 days at the respective acclimation temperature. First, exposure to Cd significantly decreased the activity of the lactate dehydrogenase (LDH) in gills of fish acclimated to 15 or 18 °C. However, an acclimation to 21 °C suppressed the inhibitory effect of Cd. Second, using a proteomic analysis by 2D-DIGE, we observed that thermal acclimation was the first parameter affecting the protein expression profile in gills of C. gobio , while subsequent Cd exposure seemed to attenuate this temperature effect. Moreover, our results showed opposite effects of these two environmental stressors at protein expression level. From the 52 protein spots displaying significant interaction effects of temperature and Cd exposure, a total of 28 different proteins were identified using nano LC–MS/MS and the Peptide and Protein Prophet algorithms of Scaffold software. The identified differentially expressed proteins can be categorized into diverse functional classes, related to protein turnover, folding and chaperoning, metabolic process, ion transport, cell signaling and cytoskeleton. Within a same functional class, we further reported that several proteins displayed reverse responses following sequential exposure to heat and Cd. This work provides insights into the molecular pathways potentially involved in heat acclimation process and the interactive effects of temperature and Cd stress in ectothermic vertebrates.
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Proteasome and antioxidant responses in Cottus gobio during a combined exposure to heat stress and cadmium
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 2011Co-Authors: Jennifer Dorts, Patrick Kestemont, Aurélie Bauwin, Sabrina Jolly, Wilfried Sanchez, Frédéric SilvestreAbstract:Abstract Temperature and trace metals are common environmental stressors, and their importance is increasing due to global climate change and anthropogenic pollution. Oxidative damage and antioxidant properties have been studied in liver and gills of the European bullhead ( Cottus gobio ) subjected to cadmium (CdCl 2 at nominal concentrations of 0.01 and 1 mg/L) for 4 days at either 15 °C or 21 °C. First, exposure to 1 mg Cd/L induced a high mortality rate (67%) in fish held at 21 °C. Regarding the antioxidant enzymes, exposure to 0.01 mg Cd/L significantly increased the activity of superoxide dismutase (SOD) and decreased the activity of glutathione reductase (GR) in liver, independently of heat stress. In gills, exposure to 21 °C resulted in a significantly increased activity of glutathione peroxidase (GPx), whereas the activity of glutathione S-transferase (GST) was significantly reduced as compared to fish exposed to 15 °C. Furthermore, regardless of Cd stress, exposure to elevated temperature resulted in a significant decrease of lipid peroxidation (LPO) level in liver and in a significant increase in the activity of chymotrypsin-like 20S proteasome in both studied tissues of C. gobio . Overall, the present results indicated that elevated temperature and cadmium exposure independently influenced the antioxidant defense system in bullhead with clear tissue-specific and stress-specific antioxidant responses. Further, elevated temperature affected the hepatic lipid peroxidation and the activity of 20S proteasome in both tissues.
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Evidence that elevated water temperature affects the reproductive physiology of the European bullhead Cottus gobio.
Fish physiology and biochemistry, 2011Co-Authors: Jennifer Dorts, Frédéric Silvestre, Gaël Grenouillet, Jessica Douxfils, Syaghalirwa N.m. Mandiki, Sylvain Milla, Patrick KestemontAbstract:Climate change is predicted to increase the average water temperature and alter the ecology and physiology of several organisms including fish species. To examine the effects of increased water temperature on freshwater fish reproduction, adult European bullhead Cottus gobio of both genders were maintained under three temperature regimes (T1: 6–10, T2: 10–14 and T3: 14–18°C) and assessed for gonad development (gonadosomatic index—GSI and gonad histology), sex steroids (testosterone—T, 17β-estradiol—E2 and 11-ketotestosterone—11-KT) and vitellogenin (alkali-labile phosphoprotein phosphorus—ALP) dynamics in December, January, February and March. The results indicate that a 8°C rise in water temperature (T3) deeply disrupted the gonadal maturation in both genders. This observation was associated with the absence of GSI peak from January to March, and low levels of plasma sex steroids compared with T1-exposed fish. Nevertheless, exposure to an increasing temperature of 4°C (T2) appeared to accelerate oogenesis with an early peak value in GSI and level of plasma T recorded in January relative to T1-exposed females. In males, the low GSI, reduced level of plasma 11-KT and the absence of GSI increase from January to March support the deleterious effects of increasing water temperature on spermatogenesis. The findings of the present study suggest that exposure to elevated temperatures within the context of climate warming might affect the reproductive success of C. gobio. Specifically, a 4°C rise in water temperature affects gametogenesis by advancing the spawning, and a complete reproductive failure is observed at an elevated temperature of 8°C.
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Ecotoxicoproteomics in gills of the sentinel fish species, Cottus gobio, exposed to perfluorooctane sulfonate (PFOS).
Aquatic toxicology (Amsterdam Netherlands), 2011Co-Authors: Jennifer Dorts, Patrick Kestemont, Martine Raes, Pierre-andré Marchand, Wendy D'hollander, Marie-laetitia Thézenas, Frédéric SilvestreAbstract:The environmental persistence, bioaccumulative tendency and potential toxicity of perfluorooctane sulfonate (PFOS) have generated great concern. This study aimed at evaluating the toxicity of short-term PFOS exposure in gills of the European bullhead Cottus gobio, a candidate sentinel species, by monitoring the response of some enzymes (citrate synthase CS, cytochrome c oxidase CCO, and lactate dehydrogenase LDH), and by undertaking a proteomic analysis using 2D-DIGE. First, a 96-h exposure to 1mg PFOS/L significantly altered the activity of mitochondrial CS and CCO. Second, 2D-DIGE gels were used to compare gills from the control fish group with tissues from fish exposed for 96h to either 0.1 or 1mg PFOS/L. From the 27 protein spots displaying significant changes in abundance following PFOS exposure, a total of 20 different proteins were identified using nano LC-MS/MS and the Peptide and Protein Prophet of Scaffold software. The differentially expressed proteins that were identified are involved in the general stress response, ubiquitin-proteasome system, energy metabolism, and actin cytoskeleton, which provide clues on the cellular pathways and components mainly affected by PFOS. Moreover, our results showed that most proteins were differentially expressed at the low but not at the high PFOS concentration. This work provides insights into the biochemical and molecular events in PFOS-induced toxicity in gill tissue, and suggests that further studies on the identified proteins could provide crucial information to better understand the mechanisms of PFOS toxicity in fish.
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Combined effects of heat stress and xenobiotic exposure in the European bullhead Cottus gobio. Clues provided by enzymatic and proteomic approaches
2011Co-Authors: Jennifer DortsAbstract:Combined exposure to elevated temperature and pollutants is an environmentally relevant scenario that is expected to become more widespread in the future with global warming. Accordingly, the general objective of the present study was to investigate the interactive effects of temperature stress and xenobiotic exposure in the European bullhead, Cottus gobio, a candidate sentinel species. Before studying the combined stresses, we have firstly characterized the toxicity of two ubiquitous contaminants in aquatic environments, i.e., cadmium (Cd) and perfluorooctane sulfonate (PFOS), in C. gobio. Exposure to high pollutants concentrations significantly altered the activity of several enzymes involved in energy metabolism, namely, citrate synthase, cytochrome c oxidase and lactate dehydrogenase, either in liver and/or in gills. Additionally, a proteomic approach was used to further our understanding of toxicity of the selected pollutants in C. gobio. The results showed that most of the identified differentially expressed proteins are associated with central aspects of the evolutionarily conserved cellular stress response and are part of the stress proteome. Secondly, as temperature and Cd are known to induce oxidative stress in aquatic organisms, their combined effects on the oxidative stress status of bullhead were assessed. Overall, we have observed that elevated temperature and Cd exposure independently influenced the antioxidant defence system with clear tissue-specific and stress-specific antioxidant responses. Further, the 20S proteasome activity was increased in response to heat stress in liver and gills. Finally, we have evaluated the effects of temperature and Cd exposure in sequential combination, i.e., fish acclimated to enhanced temperature and subsequently exposed to Cd. A combination of enzyme activity assays and protein expression patterns clearly indicated that increases in water temperature consistent with climate change predictions may modulate the ability of C. gobio to tolerate subsequent exposure to another stressor, such as Cd. Furthermore, the proteomic analysis brings clues to the potential molecular pathways involved in the acclimation process and by which a heat acclimation could interfere with an additional stress to Cd thereby highlighting shared mechanisms of action. As a corollary, the present study demonstrated that an integrative approach using enzymatic and proteomic analyses is worthwhile to better characterize the interactive effects of multiple stressors, such as temperature and metal pollution on fish species, and suggests that additional studies to link the cellular phenotype as observed here to changes at higher levels of biological organization could provide essential information to inform on organisms’ health in their natural environments.
Arne W. Nolte - One of the best experts on this subject based on the ideXlab platform.
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a genetic map of Cottus gobio pisces teleostei based on microsatellites can be linked to the physical map of tetraodon nigroviridis
Journal of Evolutionary Biology, 2005Co-Authors: Kathryn Stemshorn, Arne W. Nolte, Diethard TautzAbstract:To initiate QTL studies in the nonmodel fish Cottus gobio we constructed a genetic map based on 171 microsatellite markers. The mapping panel consisted of F1 intercrosses between two divergent Cottus lineages from the River Rhine System. Basic local alignment search tool (BLAST) searches with the flanking sequences of the microsatellite markers yielded a significant (e < 10−5) hit with the Tetraodon nigroviridis genomic sequence for 45% of the Cottus loci. Remarkably, most of these hits were due to short highly conserved noncoding stretches. These have an average length of 40 bp and are on average 92% conserved. Comparison of the map locations between the two genomes revealed extensive conserved synteny, suggesting that the Tetraodon genomic sequence will serve as an excellent genomic reference for at least the Acanthopterygii, which include evolutionarily interesting fish groups such as guppies (Poecilia), cichlids (Tilapia) or Xiphophorus (Platy). The apparent high density of short conserved noncoding stretches in these fish genomes will highly facilitate the identification of genes that have been identified in QTL mapping strategies of evolutionary relevant traits.
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A genetic map of Cottus gobio (Pisces, Teleostei) based on microsatellites can be linked to the physical map of Tetraodon nigroviridis
Journal of evolutionary biology, 2005Co-Authors: Kathryn Stemshorn, Arne W. Nolte, Diethard TautzAbstract:To initiate QTL studies in the nonmodel fish Cottus gobio we constructed a genetic map based on 171 microsatellite markers. The mapping panel consisted of F1 intercrosses between two divergent Cottus lineages from the River Rhine System. Basic local alignment search tool (BLAST) searches with the flanking sequences of the microsatellite markers yielded a significant (e
-
Direct cloning of microsatellite loci from Cottus gobio through a simplified enrichment procedure
Molecular Ecology Notes, 2005Co-Authors: Arne W. Nolte, Kathryn Stemshorn, Diethard TautzAbstract:Applying a simplified enrichment procedure, we have isolated and characterized 177 microsatellite markers for Cottus gobio L., 1758 (Cottidae, Scorpaeniformes, Teleostei). In contrast to using specific probes for the enrichment, we use genomic DNAs of unrelated organisms for cross-hybridization. This takes advantage of the fact that simple sequences are the only repetitive elements that are abundantly found in all eukaryotic genomes and that any genome usually contains all permutations of microsatellite motifs. This cross-hybridization principle was employed to enrich genomic libraries of Cottus DNA to obtain a large number of nonredundant microsatellite markers without further screening procedures.
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phylogeography of the bullhead Cottus gobio pisces teleostei cottidae suggests a pre pleistocene origin of the major central european populations
Molecular Ecology, 2000Co-Authors: Claudia C. Englbrecht, Arne W. Nolte, Jörg Freyhof, K. Rassmann, Ulrich K. Schliewen, Diethard TautzAbstract:The bullhead Cottus gobio is a small, bottom-dwelling fish consisting of populations that have not been subject to transplantations or artificial stocking. It is therefore an ideal model species for studying the colonization history of central European freshwater systems, in particular with respect to the possible influences of the Pleistocene glaciation cycles. We sampled Cottus populations across most of its distribution range, with a special emphasis on southern Germany where the major European drainage systems are in closest contact. Mitochondrial D-loop sequencing of more than 400 specimens and phylogenetic network analysis allowed us to draw a detailed picture of the colonization of Europe by C. gobio. Moreover, the molecular distances between the haplotypes enabled us to infer an approximate time frame for the origin of the various populations. The founder population of C. gobio stems apparently from the Paratethys and invaded Europe in the Pliocene. From there, the first colonization into central Europe occurred via the ancient lower Danube, with a separate colonization of the eastern European territories. During the late Pliocene, one of the central European populations must have reached the North Sea in a second step after which it then started to colonize the Atlantic drainages via coastal lines. Accordingly, we found very distinct populations in the upper and lower Rhine, which can be explained by the fact that the lower Rhine was disconnected from the upper Rhine until approximately 1 million years ago (Ma). More closely related, but still distinct, populations were found in the Elbe, the Main and the upper Danube, all presumably of Pleistocene origin. Intriguingly, they have largely maintained their population identity, despite the strong disturbance caused by the glaciation cycles in these areas. On the other hand, a mixing of populations during postglacial recolonization could be detected in the lower Rhine and its tributaries. However, the general pattern that emerges from our analysis suggests that the glaciation cycles did not have a major impact on the general population structure of C. gobio in central Europe.
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Phylogeography of the bullhead Cottus gobio (Pisces: Teleostei: Cottidae) suggests a pre‐Pleistocene origin of the major central European populations
Molecular ecology, 2000Co-Authors: Claudia C. Englbrecht, Arne W. Nolte, Jörg Freyhof, K. Rassmann, Ulrich K. Schliewen, Diethard TautzAbstract:The bullhead Cottus gobio is a small, bottom-dwelling fish consisting of populations that have not been subject to transplantations or artificial stocking. It is therefore an ideal model species for studying the colonization history of central European freshwater systems, in particular with respect to the possible influences of the Pleistocene glaciation cycles. We sampled Cottus populations across most of its distribution range, with a special emphasis on southern Germany where the major European drainage systems are in closest contact. Mitochondrial D-loop sequencing of more than 400 specimens and phylogenetic network analysis allowed us to draw a detailed picture of the colonization of Europe by C. gobio. Moreover, the molecular distances between the haplotypes enabled us to infer an approximate time frame for the origin of the various populations. The founder population of C. gobio stems apparently from the Paratethys and invaded Europe in the Pliocene. From there, the first colonization into central Europe occurred via the ancient lower Danube, with a separate colonization of the eastern European territories. During the late Pliocene, one of the central European populations must have reached the North Sea in a second step after which it then started to colonize the Atlantic drainages via coastal lines. Accordingly, we found very distinct populations in the upper and lower Rhine, which can be explained by the fact that the lower Rhine was disconnected from the upper Rhine until approximately 1 million years ago (Ma). More closely related, but still distinct, populations were found in the Elbe, the Main and the upper Danube, all presumably of Pleistocene origin. Intriguingly, they have largely maintained their population identity, despite the strong disturbance caused by the glaciation cycles in these areas. On the other hand, a mixing of populations during postglacial recolonization could be detected in the lower Rhine and its tributaries. However, the general pattern that emerges from our analysis suggests that the glaciation cycles did not have a major impact on the general population structure of C. gobio in central Europe.
Jean-nicolas Beisel - One of the best experts on this subject based on the ideXlab platform.
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glochidial infestation by the endangered mollusc unio crassus in rivers of north eastern france phoxinus phoxinus and Cottus gobio as primary fish hosts
Aquatic Conservation-marine and Freshwater Ecosystems, 2016Co-Authors: Florent Lamand, Kevin Roche, Jean-nicolas BeiselAbstract:The thick-shelled river mussel Unio crassus is an endangered freshwater bivalve species with a life cycle that includes an obligate glochidial larval stage that attaches to the gills of freshwater fish for several days to months. Fish hosts were identified in nine north-eastern French rivers in order to improve unionid conservation management. Eurasian minnow Phoxinus phoxinus was the most important host at all study sites, with prevalence ranging from 15–100% (122 of 238 individuals infested), and bullhead Cottus gobio usually the second most important (prevalence 0–73%; 37 of 121). Other host species had a relatively low rate of infestation: chub Squalius cephalus (5 of 41), roach Rutilus rutilus (1 of 23), gudgeon gobio gobio (2 of 50), stone loach Barbatula barbatula (2 of 58) and dace Leuciscus leuciscus (1 of 19). No glochidia were found on spirlin Alburnoides bipunctatus (0 of 24). Parasitic load usually ranged between one and three per fish (60% of cases), and rarely exceeded 10. First results suggest that U. crassus glochidia influence the weight:length ratio of P. phoxinus but not C. gobio, with infested fish being heavier. To date, the ecological function of P. phoxinus (and C. gobio) in small streams has been largely underestimated or ignored as the species is small, common and of no interest to anglers. There is increasing evidence, however, that P. phoxinus deserves conservation management owing to the essential role it plays in the reproduction, development and distribution of U. crassus. Copyright © 2016 John Wiley & Sons, Ltd.
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Glochidial infestation by the endangered mollusc Unio crassus in rivers of north‐eastern France: Phoxinus phoxinus and Cottus gobio as primary fish hosts
Aquatic Conservation: Marine and Freshwater Ecosystems, 2016Co-Authors: Florent Lamand, Kevin Roche, Jean-nicolas BeiselAbstract:The thick-shelled river mussel Unio crassus is an endangered freshwater bivalve species with a life cycle that includes an obligate glochidial larval stage that attaches to the gills of freshwater fish for several days to months. Fish hosts were identified in nine north-eastern French rivers in order to improve unionid conservation management. Eurasian minnow Phoxinus phoxinus was the most important host at all study sites, with prevalence ranging from 15–100% (122 of 238 individuals infested), and bullhead Cottus gobio usually the second most important (prevalence 0–73%; 37 of 121). Other host species had a relatively low rate of infestation: chub Squalius cephalus (5 of 41), roach Rutilus rutilus (1 of 23), gudgeon gobio gobio (2 of 50), stone loach Barbatula barbatula (2 of 58) and dace Leuciscus leuciscus (1 of 19). No glochidia were found on spirlin Alburnoides bipunctatus (0 of 24). Parasitic load usually ranged between one and three per fish (60% of cases), and rarely exceeded 10. First results suggest that U. crassus glochidia influence the weight:length ratio of P. phoxinus but not C. gobio, with infested fish being heavier. To date, the ecological function of P. phoxinus (and C. gobio) in small streams has been largely underestimated or ignored as the species is small, common and of no interest to anglers. There is increasing evidence, however, that P. phoxinus deserves conservation management owing to the essential role it plays in the reproduction, development and distribution of U. crassus. Copyright © 2016 John Wiley & Sons, Ltd.
